1,902 results match your criteria expression lh-beta

Sequential preovulatory expression of a gonadotropin-releasing hormone-inducible gene, Nr4a3, and its suppressor Anxa5 in the pituitary gland of female rats.

J Reprod Dev 2021 Apr 10. Epub 2021 Apr 10.

Laboratory of Veterinary Physiology, School of Veterinary Medicine, Kitasato University, Aomori 034-8628, Japan.

Functional relationship between nuclear receptor subfamily 4 group A member 3 (Nr4a3) and annexin A5 (Anxa5), which are two gonadotropin-releasing hormone (GnRH)-inducible genes, has been established while evaluating pituitary gonadotropes in relation to follicle-stimulating hormone beta (Fshb) expression. However, the physiological variations that arise due to the differential expression of these genes in the pituitary gland during rat estrous cycle remain unknown. This study aimed to evaluate the Nr4a3 and Anxa5 mRNA expression during the estrous cycle in rats in comparison with the expression of the gonadotropin subunit genes, luteinizing hormone beta (Lhb) and Fshb. Read More

View Article and Full-Text PDF

Granulosa cell genes that regulate ovarian follicle development beyond the antral stage: The role of estrogen receptor β.

Mol Cell Endocrinol 2021 Mar 4;528:111212. Epub 2021 Mar 4.

Department of Pathology and Laboratory Medicine, Kansas City, KS, USA; Institute for Reproduction and Perinatal Research, University of Kansas Medical Center, Kansas City, KS, USA. Electronic address:

Follicle development beyond the preantral stage is dependent on gonadotropins. FSH signaling is crucial for the advancement of preantral follicles to the antral stage, and LH signaling is essential for further maturation of preovulatory follicles. Estrogen is intricately tied to gonadotropin signaling during the advanced stages of folliculogenesis. Read More

View Article and Full-Text PDF

Early exposure to paracetamol reduces level of testicular testosterone and changes gonadal expression of genes relevant for steroidogenesis in rats offspring.

Drug Chem Toxicol 2021 Mar 3:1-8. Epub 2021 Mar 3.

Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Centre for Preclinical Research and Technology CePT, Warsaw, Poland.

In this study, we investigated the effects of early paracetamol treatment on the testicular level of testosterone and expression of genes important for steroid biosynthesis and reproduction in male rats offspring. Rats were continuously exposed to paracetamol at doses of 5 or 15 mg/kg b.w. Read More

View Article and Full-Text PDF

Di-n-butyl phthalate diminishes testicular steroidogenesis by blocking the hypothalamic-pituitary-testicular axis: relationship with germ cell apoptosis in Japanese quail.

Reprod Fertil Dev 2021 Mar;33(5):319-327

Laboratory of Veterinary Anatomy, Department of Veterinary Medicine, Okayama University of Science, Imabari, Ehime 794-8555, Japan; and Department of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

Although di-n-butyl phthalate (DBP) induces germ cell apoptosis, the underlying mechanism is not yet clear in quail. In this study, prepubertal quails were given a single dose of 500mg kg-1 DBP by gavage and were then killed 3, 6 and 24h after treatment. There was a significant reduction in intratesticular testosterone (ITT) concentrations and testicular steroidogenic enzyme mRNA expression and a significant increase in germ cell apoptosis in DBP-treated compared with control quails at all time points. Read More

View Article and Full-Text PDF

Low dose of fire retardant, 2,2',4,4'-tetrabromodiphenyl ether (BDE47), stimulates the proliferation and differentiation of progenitor Leydig cells of male rats during prepuberty.

Toxicol Lett 2021 May 10;342:6-19. Epub 2021 Feb 10.

Department of Anesthesiology, Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, China. Electronic address:

2,2',4,4'-Tetrabromodiphenyl ether (BDE47), a flame retardant, is extensively distributed in the food chain. However, whether BDE47 affects Leydig cell development during prepuberty remains unclear. BDE47 was daily gavaged to 21-day-old Sprague-Dawley male rats with 0 (corn oil), 0. Read More

View Article and Full-Text PDF

The relationship between abnormal Core binding factor-β expression in human cartilage and osteoarthritis.

BMC Musculoskelet Disord 2021 Feb 11;22(1):174. Epub 2021 Feb 11.

Department of Orthopaedics, The Affiliated Hospital of Guizhou Medical University, Guiyang, China.

Background: This study aimed to investigate the effect of abnormal Core binding factor-β expression on proliferation, differentiation and apoptosis of chondrocytes, and elucidate the relationship between Core binding factor-β and osteoarthritis-related markers and degenerative joint disease.

Methods: Cartilage tissues, from healthy subjects and patients with osteoarthritis, were collected for histology and expression of Core binding factor-β, MMP-13, IL-1β, COMP, and YKL-40. Human articular chondrocytes were cultured in vitro, and a viral vector was constructed to regulate cellular Core binding factor-β expression. Read More

View Article and Full-Text PDF
February 2021

Nonsteroidal anti-inflammatory drugs act as endocrine disruptors in Astyanax lacustris (Teleostei: Characidae) reproduction: An ex vivo approach.

Aquat Toxicol 2021 Jan 29;232:105767. Epub 2021 Jan 29.

Escola de Ciências da Saúde, Universidade Anhembi Morumbi - R. Dr. Almeida Lima, 1134 - Parque da Mooca, 03164-000, São Paulo, SP, Brazil. Electronic address:

Pharmaceutical products can act as endocrine-disrupting compounds (EDCs), affecting the physiological processes of animals, such as development or reproduction. This study aimed to investigate the influence of different concentrations of nonsteroidal anti-inflammatory drugs (NSAIDs) diclofenac (DCF) and ibuprofen (IBU) alone and mixed (MIX) on gonadotropin gene expression and gonadal steroid release using Astyanax lacustris pituitary and testes explant systems, respectively. The explant organs were maintained for 12 h in Leibovitz (L-15) medium supplemented with 0, 0. Read More

View Article and Full-Text PDF
January 2021

Tribbles Pseudokinase 2 (TRIB2) Regulates Expression of Binding Partners in Bovine Granulosa Cells.

Int J Mol Sci 2021 Feb 3;22(4). Epub 2021 Feb 3.

Département de Biomédecine Vétérinaire, Centre de Recherche en Reproduction et Fertilité (CRRF), Faculté de Médecine Vétérinaire, Université de Montréal, 3200 Rue Sicotte, St-Hyacinthe, QC J2S 2M2, Canada.

Members of the Tribbles (TRIB) family of pseudokinases are critical components of intracellular signal transduction pathways in physiological and pathological processes. TRIBs, including TRIB2, have been previously shown as signaling mediators and scaffolding proteins regulating numerous cellular events such as proliferation, differentiation and cell death through protein stability and activity. However, the signaling network associated with TRIB2 and its binding partners in granulosa cells during ovarian follicular development is not fully defined. Read More

View Article and Full-Text PDF
February 2021

Characterization of stem cells from human ovarian follicular fluid; a potential source of autologous stem cell for cell-based therapy.

Hum Cell 2021 Mar 4;34(2):300-309. Epub 2021 Feb 4.

Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

Human ovarian follicular fluid (HOFF) contains proteins, extracellular matrixes necessary for growth and maturation of oocytes as well as granulosa cells. Epithelial cells and stem cells can be isolated from HOFF. However, information regarding stem cells derived from HOFF is still lacking. Read More

View Article and Full-Text PDF

Molecular economy of nature with two thyrotropins from different parts of the pituitary: pars tuberalis thyroid-stimulating hormone and pars distalis thyroid-stimulating hormone.

Sibel Ertek

Arch Med Sci 2021 5;17(1):189-195. Epub 2021 Jan 5.

Department of Endocrinology and Metabolic Diseases, Memorial Ankara Hospital, Ankara, Turkey.

Thyrotropin (TSH) is classically known to be regulated by negative feedback from thyroid hormones and stimulated by thyrotropin-releasing hormone (TRH) from the hypothalamus. At the end of the 1990s, studies showed that thyrotroph cells from the pars tuberalis (PT) did not have TRH receptors and their TSH regulation was independent from TRH stimulation. Instead, PT-thyrotroph cells were shown to have melatonin-1 (MT-1) receptors and melatonin secretion from the pineal gland stimulates TSH-β subunit formation in PT. Read More

View Article and Full-Text PDF
January 2021

LH upregulates connexin 43 expression in granulosa cells by activating the Wnt/β-catenin signalling pathway.

Reprod Fertil Dev 2021 Feb;33(3):239-244

Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, School of Basic Medical Sciences, Department II of Surgical Oncology, Tumor Hospital of General Hospital of Ningxia Medical University, Ningxia Medical University, Shengli Street 1160#, Yinchuan 750004, China; and Corresponding authors. Email:

Connexin (Cx) 43 is the most widely expressed gap junction protein in follicle granulosa cells and plays an important role in follicle development and growth. The aims of this study were to investigate the effects of LH on the expression of Cx43 and key proteins in the downstream Wnt-β/catenin signalling pathway and to explore the mechanism underlying the regulation of Cx43 expression in granulosa cells. Primary culture granulosa cells were obtained from 21-day-old Sprague-Dawley rats, and were treated with different concentrations of LH (150, 300 and 600 IU L-1). Read More

View Article and Full-Text PDF
February 2021

TGFBR3L-An Uncharacterised Pituitary Specific Membrane Protein Detected in the Gonadotroph Cells in Non-Neoplastic and Tumour Tissue.

Cancers (Basel) 2020 Dec 31;13(1). Epub 2020 Dec 31.

Department of Immunology, Genetics and Pathology, Uppsala University, Dag Hammarskjöldsväg 20, 752 37 Uppsala, Sweden.

Here, we report the investigation of transforming growth factor beta-receptor 3 like (TGFBR3L), an uncharacterised pituitary specific membrane protein, in non-neoplastic anterior pituitary gland and pituitary neuroendocrine tumours. A polyclonal antibody produced within the Human Protein Atlas project (HPA074356) was used for TGFBR3L staining and combined with SF1 and FSH for a 3-plex fluorescent protocol, providing more details about the cell lineage specificity of TGFBR3L expression. A cohort of 230 pituitary neuroendocrine tumours were analysed. Read More

View Article and Full-Text PDF
December 2020

In vitro differentiation of theca cells from ovarian cells isolated from postmenopausal women.

Hum Reprod 2020 Dec;35(12):2793-2807

Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium.

Study Question: Can human theca cells (TCs) be differentiated in vitro?

Summary Answer: It is possible to differentiate human TCs in vitro using a medium supplemented with growth factors and hormones.

What Is Known Already: There are very few studies on the origin of TCs in mammalian ovaries. Precursor TCs have been described in neonatal mice ovaries, which can differentiate into TCs under the influence of factors from oocytes and granulosa cells (GCs). Read More

View Article and Full-Text PDF
December 2020

CITED4 mediates proliferation, apoptosis and steroidogenesis of Hu sheep granulosa cells in vitro.

Reproduction 2021 Mar;161(3):255-267

Jiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing, China.

Being a novel target of luteinizing hormone (LH), the effect of CREB-binding protein/P300-interacting trans-activator with ED-rich tail member 4 (CITED4) gene on the proliferation, apoptosis, and steroidogenesis of ovarian granulosa cells (GCs) in Hu sheep was investigated. The presence of CITED4, CREB-binding protein (CBP), CCAAT/enhancer-binding protein alpha (C/EBPα) and -beta (C/EBPβ) proteins was demonstrated in GCs and luteal cells. CITED4 protein in GCs was induced by LH, and CITED4 overexpression moderately increased GC responses to LH. Read More

View Article and Full-Text PDF

Fermented Maillard reaction products attenuate stress-induced testicular dysfunction in mice.

J Dairy Sci 2021 Feb 30;104(2):1384-1393. Epub 2020 Nov 30.

Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Korea. Electronic address:

Chronic stress can cause psychological diseases and affect male fertility and the reproductive system. Maillard reaction of milk proteins improves their functional and nutritional properties through modification of proteins. Previously, we determined that Maillard reaction product (MRP) from milk casein and MRP fermented (FMRP) with Lactobacillus rhamnosus 4B15 (4B15) had anti-anxiolytic effects in mice under chronic stress. Read More

View Article and Full-Text PDF
February 2021

Analysis of the immune response to sciatic nerve injury identifies efferocytosis as a key mechanism of nerve debridement.

Elife 2020 12 2;9. Epub 2020 Dec 2.

Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, United States.

Sciatic nerve crush injury triggers sterile inflammation within the distal nerve and axotomized dorsal root ganglia (DRGs). Granulocytes and pro-inflammatory Ly6C monocytes infiltrate the nerve first and rapidly give way to Ly6C inflammation-resolving macrophages. In axotomized DRGs, few hematogenous leukocytes are detected and resident macrophages acquire a ramified morphology. Read More

View Article and Full-Text PDF
December 2020

Expression of lysophosphatidic acid receptor 1 in the adult female mouse pituitary gland.

Neurosci Lett 2021 01 19;741:135506. Epub 2020 Nov 19.

Department of Life Science, Kindai University, 3-4-1 Kowakae, Higashiosaka, Osaka 577-8502, Japan.

Lysophosphatidic acid receptor 1 (LPA) is a receptor of lysophosphatidic acid (LPA). The present study investigated Lpar mRNA expression in the mouse pituitary gland by RT-PCR, in situ hybridization, and immunohistochemistry. Lpar mRNA was abundantly expressed in the pituitary gland. Read More

View Article and Full-Text PDF
January 2021

Expression of genes that regulate follicle development and maturation during ovarian stimulation in poor responders.

Reprod Biomed Online 2021 Jan 25;42(1):248-259. Epub 2020 May 25.

Unit for Human Reproduction, Laboratory of Genetics, 1st Department of Obstetrics and Gynaecology, School of Medicine, Aristotle University of Thessaloniki, Papageorgiou General Hospital, Ring Road, Nea Efkarpia Thessaloniki 56403, Greece.

Research Question: Sex hormone-binding globulin (SHBG), androgen receptor (AR), LH beta polypeptide (LHB), progesterone receptor membrane component 1 (PGRMC1) and progesterone receptor membrane component 2 (PGRMC2) regulate follicle development and maturation. Their mRNA expression was assessed in peripheral blood mononuclear cells (PBMC) of normal and poor responders, during ovarian stimulation.

Design: Fifty-two normal responders and 15 poor responders according to the Bologna criteria were enrolled for IVF and intracytoplasmic sperm injection and stimulated with 200 IU of follitrophin alpha and gonadotrophin-releasing hormone antagonist. Read More

View Article and Full-Text PDF
January 2021

Rapamycin Ameliorates Cognitive Impairments and Alzheimer's Disease-Like Pathology with Restoring Mitochondrial Abnormality in the Hippocampus of Streptozotocin-Induced Diabetic Mice.

Neurochem Res 2021 Feb 2;46(2):265-275. Epub 2020 Nov 2.

Department of Clinical Research Center, The First Affliated Hospital of Guizhou University of Traditional Chinese Medicine, Baoshan Road No.71, Guiyang, 550001, Guizhou, China.

Alzheimer's disease (AD) and diabetes mellitus (DM) share common pathophysiological findings, in particular, the mammalian target of rapamycin (mTOR) has been strongly implied to link to AD, while it also plays a key role in the insulin signaling pathway. However, the mechanism of how DM and AD is coupled remains elusive. In the present study, we found that streptozotocin (STZ)-induced DM mice significantly increased the levels P-mTOR Ser2448, P-p70S6K Thr389, P-tau Ser356 and Aβ levels (Aβ oligomer/monomer), as well as the levels of Drp1 and p-Drp1 S616 (mitochondrial fission proteins) are increased, whereas no change was found in the expression of Opa1, Mfn1 and Mfn2 (mitochondrial fusion proteins) compared with control mice. Read More

View Article and Full-Text PDF
February 2021

Changes in nitric oxide, carbon monoxide, hydrogen sulfide and male reproductive hormones in response to chronic restraint stress in rats.

Amira Moustafa

Free Radic Biol Med 2021 Jan 29;162:353-366. Epub 2020 Oct 29.

Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, 44519, Zagazig, Egypt. Electronic address:

Gasotrasmitters are endogenously synthesized gaseous molecules that are engaged in cellular physiological and pathological processes. Stress influences various physiological aspects of an organism and amends a normal system's functions, including those of the reproductive system. This study aims to investigate the effect of long-term exposure to restraint stress on the male reproductive system as well as the possible impact of stress on the levels of nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (HS), and the expression of their producing-enzymes. Read More

View Article and Full-Text PDF
January 2021

Effects of Kiss2 on the Expression of Gonadotropin Genes in the Pituitary of Nile Tilapia ().

Dev Reprod 2020 Sep 30;24(3):149-158. Epub 2020 Sep 30.

Dept. of Aquatic Life Medical Sciences, Sunmoon University, Asan 31460, Korea.

Kisspeptin, expressed mainly in the hypothalamus, stimulates gonadotropin-releasing hormone neurons to facilitate reproduction. In some model animals, the kisspeptin is also expressed in the pituitary. Recently, a pathway has been suggested in which kisspeptin acts directly on the pituitary to secretion of gonadotropin in mammals. Read More

View Article and Full-Text PDF
September 2020

Exposure to di-n-octyl phthalate during puberty induces hypergonadotropic hypogonadism caused by Leydig cell hyperplasia but reduced steroidogenic function in male rats.

Ecotoxicol Environ Saf 2021 Jan 16;208:111432. Epub 2020 Oct 16.

Department of Obstetrics and Gynecology, the Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China; Department of Anesthesiology, the Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China. Electronic address:

Humans are exposed to phthalates ubiquitously, which may threaten health. However, whether di-n-octyl phthalate can prevent pubertal sexual maturity is still elusive. In this study, male Sprague Dawley rats (age 35 days) were treated daily by gavage with 0, 10, 100, and 1000 mg/kg body weight of di-n-octyl phthalate from day 35 to day 49 after birth. Read More

View Article and Full-Text PDF
January 2021

Transcriptome characterization of BPG axis and expression profiles of ovarian steroidogenesis-related genes in the Japanese sardine.

BMC Genomics 2020 Sep 29;21(1):668. Epub 2020 Sep 29.

Hakatajima Field Station, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, Kinoura, Imabari, Ehime, 794-2305, Japan.

Background: The clupeoid fishes are ecologically and commercially important fish species worldwide that exhibit a high level of population fluctuation, accompanied by alteration of reproductive traits. However, knowledge about their reproductive physiology in order to understand mechanisms underlying such population dynamics is limited. The endocrine system along with the brain-pituitary-gonadal (BPG) axis is critical for regulating reproduction. Read More

View Article and Full-Text PDF
September 2020

The Impact of Controlled Ovarian Stimulation Hormones on the Metabolic State and Endocannabinoid System of Human Cumulus Cells.

Int J Mol Sci 2020 Sep 27;21(19). Epub 2020 Sep 27.

Department of Life and Environmental Sciences, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.

Different Follicle Stimulating Hormone (FSH) formulation and Luteinizing Hormone (LH) are used in Assisted Reproductive Technology (ART) to induce follicles development and oocytes maturation, but it is still under debate which protocol is to be preferred. In the present study, the different effects on cumulus cells (CCs) of three controlled ovarian stimulation (COS) protocols, based on urinary FSH, recombinant FSH, or human Menopausal Gonadotropin (hMG) administration, were assessed. CCs were obtained from 42 normal-responders women undergoing COS, randomly divided into three groups according to the used gonadotropin formulation. Read More

View Article and Full-Text PDF
September 2020

Effect of Acute and Prolonged Inflammation on the Gene Expression of Proinflammatory Cytokines and Their Receptors in the Anterior Pituitary Gland of Ewes.

Int J Mol Sci 2020 Sep 21;21(18). Epub 2020 Sep 21.

The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 03-105 Jabłonna, Poland.

An acute and prolonged inflammation inhibits the reproduction process by the disruption of the neurohormonal activity of the hypothalamic-pituitary-gonadal axis. It is thought that these changes may be caused by proinflammatory cytokines, i.e. Read More

View Article and Full-Text PDF
September 2020

Effects of allopregnanolone on central reproductive functions in sheep under natural and stressful conditions.

Theriogenology 2020 Dec 11;158:138-147. Epub 2020 Sep 11.

Department of Animal Physiology, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3 St, 05-110, Jablonna, Poland. Electronic address:

Reproductive functions may be affected by internal and external factors that are integrated in the central nervous system (CNS). Stressful stimuli induce the neuroendocrine response of the hypothalamic-pituitary-adrenal axis, as well as the synthesis of the neurosteroid allopregnanolone (AL) in the brain. This study tested the hypothesis that centrally administered AL could affect the expression of certain genes involved in reproductive functions at the hypothalamus and pituitary levels, as well as pulsatile gonadotropin secretion in sheep under both natural and stressful conditions. Read More

View Article and Full-Text PDF
December 2020

Pituitary Actions of EGF on Gonadotropins, Growth Hormone, Prolactin and Somatolactins in Grass Carp.

Biology (Basel) 2020 Sep 8;9(9). Epub 2020 Sep 8.

College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.

In mammals, epidermal growth factor (EGF) plays a vital role in both pituitary physiology and pathology. However, the functional role of EGF in the regulation of pituitary hormones has rarely reported in teleost. In our study, using primary cultured grass carp pituitary cells as an in vitro model, we examined the effects of EGF on pituitary hormone secretion and gene expression as well as the post-receptor signaling mechanisms involved. Read More

View Article and Full-Text PDF
September 2020

β-Cyclodextrin-modified hyaluronic acid-based supramolecular self-assemblies for pH- and esterase- dual-responsive drug delivery.

Carbohydr Polym 2020 Oct 18;246:116654. Epub 2020 Jun 18.

Shaanxi Key Laboratory of Polymer Science & Technology, OME Key Laboratory of Supernormal Material Physics & Chemistry, School of Chemistry and Chemical Engineering, Northwestern Polytechnical University, Xi'an, 710072, China. Electronic address:

Although some drug-based supramolecular systems have been constructed to overcome multidrug resistance and enhance the bioavailability of chemical drugs, strengthening the specific stimuli-responsive and active targeting ability of these systems is still a major challenge. In this paper, the synthesis and self-assembly behaviour of supramolecular self-assemblies with active targeting β-cyclodextrin-modified hyaluronic acid (HA-CD) and drug-drug conjugates (curcumin-oxoplatin, Cur-Pt) as building moieties were carefully investigated. Notably, the curcumin was chosen not only as the chemical anti-cancer drug, but also acted as the guest molecule which could be included into CD cavity to form host-guest interaction-based supramolecular assemblies. Read More

View Article and Full-Text PDF
October 2020

Bisphenol-A analogue (bisphenol-S) exposure alters female reproductive tract and apoptosis/oxidative gene expression in blastocyst-derived cells.

Iran J Basic Med Sci 2020 May;23(5):576-585

Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

Objectives: One of the major endocrine-disrupting chemicals, bisphenol-S (BPS) has replaced bisphenol-A due to public health anxiety. The present study evaluated low dosage BPS effect on female reproductive potential, hormonal disruption, and gene expression pathways of blastocyst-derived cells.

Materials And Methods: NMRI female mice (5-6 weeks) in the estrous stage were chosen following vaginal smear examination for estrus cycle detection and BPS (0, 1, 5, 10, 50 and 100 µg/kg) was administrated subcutaneously for twenty-one consecutive days. Read More

View Article and Full-Text PDF

The microRNA-200 cluster on chromosome 23 is required for oocyte maturation and ovulation in zebrafish†.

Biol Reprod 2020 10;103(4):769-778

College of Fisheries, Huazhong Agricultural University, Wuhan, China.

The reproductive process is usually controlled by the hypothalamic-pituitary-gonad axis in vertebrates, while Kiss/gonadotropin-releasing hormone (GnRH) system in the hypothalamus is required for mammalian reproduction but dispensable for fish reproduction. The regulation of follicle stimulating hormone/luteinizing hormone (LH) expression in fish species is still unknown. Here, we identified miR-200s on chromosome 23 (chr23-miR-200s) as important regulators for female zebrafish reproduction. Read More

View Article and Full-Text PDF
October 2020