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Ketogenic diet-mediated steroid metabolism reprogramming improves the immune microenvironment and myelin growth in spinal cord injury rats according to gene and co-expression network analyses.

Aging (Albany NY) 2021 May 6;13. Epub 2021 May 6.

Department of Rehabilitation Medicine, Peking University Third Hospital, Beijing 100191, China.

The ketogenic diet has been widely used in the treatment of various nervous system and metabolic-related diseases. Our previous research found that a ketogenic diet exerts a protective effect and promotes functional recovery after spinal cord injury. However, the mechanism of action is still unclear. Read More

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Early time-point cell morphology classifiers successfully predict human bone marrow stromal cell differentiation modulated by fiber density in nanofiber scaffolds.

Biomaterials 2021 Apr 26;274:120812. Epub 2021 Apr 26.

National Institute of Standards & Technology, Biosystems & Biomaterials Division, 100 Bureau Dr. Stop 8543, Gaithersburg, MD, 20899, USA. Electronic address:

Nanofiber scaffolds can induce osteogenic differentiation and cell morphology alterations of human bone marrow stromal cells (hBMSCs) without introduction of chemical cues. In this study, we investigate the predictive power of day 1 cell morphology, quantified by a machine learning based method, as an indicator of osteogenic differentiation modulated by nanofiber density. Nanofiber scaffolds are fabricated via electrospinning. Read More

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Quercetin inhibits invasion and angiogenesis of esophageal cancer cells.

Pathol Res Pract 2021 Apr 28;222:153455. Epub 2021 Apr 28.

Department of Gastroenterology, Renmin Hospital, Hubei University of Medicine, Shiyan 442000, Hubei Province, China.

Background: Esophageal carcinoma has poor prognosis and novel therapies for esophageal carcinoma are urgently needed. Quercetin is a natural flavonoid compound that can be found in many foods. In this study, we investigated the effects of quercetin on invasion and angiogenesis of esophageal cancer cells. Read More

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Redox sensor QSOX1 regulates plant immunity by targeting GSNOR to modulate ROS generation.

Mol Plant 2021 May 4. Epub 2021 May 4.

Division of Applied Life Sciences (BK21) and PMBBRC, Gyeongsang National University, Jinju 52828, Korea; College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, P.R.China. Electronic address:

Reactive oxygen signaling regulates numerous biological processes, including stress responses in plants. Redox sensors transduce reactive oxygen signals into cellular responses. We present biochemical evidence that a plant quiescin sulfhydryl oxidase homolog (QSOX1) is a redox sensor that negatively regulates immunity against a bacterial pathogen. Read More

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A survey of the mouse hindbrain in the fed and fasted state using single-nucleus RNA sequencing.

Mol Metab 2021 May 4:101240. Epub 2021 May 4.

MRC Metabolic Diseases Unit, University of Cambridge Metabolic Research Laboratories, Institute of Metabolic Science, Addenbrooke's Hospital, Cambridge, UK. Electronic address:

Objective: The area postrema (AP) and the nucleus tractus solitaris (NTS), located in the hindbrain, are key nuclei that sense and integrate peripheral nutritional signals and, consequently, regulate feeding behaviour. While single cell transcriptomics have been used in mice to reveal the gene expression profile and heterogeneity of key hypothalamic populations, similar in-depth studies have not yet been performed in the hindbrain.

Methods: Using single-nucleus RNA sequencing, we provide a detailed survey of 16,034 cells within the AP and NTS of the mouse, in the fed and fasted state. Read More

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mA demethylase ALKBH5 suppresses proliferation and migration of enteric neural crest cells by regulating TAGLN in Hirschsprung's disease.

Life Sci 2021 May 4:119577. Epub 2021 May 4.

Department of Pediatric Surgery, Children's Hospital of Nanjing Medical University, Nanjing, Jiangsu, China. Electronic address:

Objectives: This study aims to investigate the role of demethylase ALKBH5 mediated demethylation of TAGLN mRNA in the occurrence of Hirschsprung's disease (HSCR), and to clarify how ALKBH5 reduces the mA level of TAGLN mRNA and inhibits its degradation, thereby inhibiting the proliferation and migration of neural crest cells, and potentially contributing to the occurrence of HSCR.

Material And Methods: Quantitative real-time PCR (qRT-PCR) and Western-Blot (WB) were conducted to test the expression level of ALKBH5 and TAGLN genes. Cell function assays were adopted to detect cell phenotypes. Read More

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ADORA1 promotes nasopharyngeal carcinoma cell progression through regulation of PI3K/AKT/GSK-3β/β-catenin signaling.

Life Sci 2021 May 4:119581. Epub 2021 May 4.

Department of Radiation Oncology, Yue Bei People's Hospital, Shaoguan, Guangdong, China.

Aims: For most human cancers, the expression pattern and biological function of ADORA1 (Adenosine A1 Receptor) are largely unknown. This study has been designed to explore the clinical significance and the mechanism of ADORA1 in nasopharyngeal carcinoma (NPC) cells.

Materials And Methods: The level of ADORA1 in NPC and its adjacent tissues was analyzed by IHC, real-time PCR and western blotting. Read More

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Irisin attenuates lipopolysaccharide-induced acute lung injury by downregulating inflammatory cytokine expression through miR-199a-mediated Rad23b overexpression.

Exp Cell Res 2021 May 4:112593. Epub 2021 May 4.

Department of Emergency Surgery, Shaanxi Provincial People's Hospital, Xi'an, 710068, China. Electronic address:

Aims: Acute lung injury (ALI) is a leading cause of mortality as a result of inflammatory cytokine overexpression and increased rates of apoptosis. Therapies for ALI are yet to be thoroughly investigated. Recent evidence has shown that irisin exerts protective effects against many types of pathologies. Read More

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Trastuzumab deruxtecan (DS-8201) in patients with HER2-expressing metastatic colorectal cancer (DESTINY-CRC01): a multicentre, open-label, phase 2 trial.

Lancet Oncol 2021 May 4. Epub 2021 May 4.

National Cancer Center Hospital East, Kashiwa, Japan. Electronic address:

Background: HER2 amplification has been identified in 2-3% of patients with colorectal cancer, although there are currently no approved HER2-targeted therapies for colorectal cancer. We aimed to study the antitumour activity and safety of trastuzumab deruxtecan (an antibody-drug conjugate of humanised anti-HER2 antibody with topoisomerase I inhibitor payloads) in patients with HER2-expressing metastatic colorectal cancer.

Methods: DESTINY-CRC01 is an open-label, phase 2 study that recruited patients from 25 clinics and hospitals in Italy, Japan, Spain, the UK, and the USA. Read More

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SmKFB5 Protein Regulate Phenolic Acids Biosynthesis via Controlling the Degradation of Phenylalanine Ammonia-Lyase in Salvia miltiorrhiza.

J Exp Bot 2021 May 7. Epub 2021 May 7.

Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China.

Phenolic acids are the major secondary metabolites and significant bioactive constituents of medicinal plant Salvia miltiorrhiza. Many enzyme-encoding genes and transcription factors involved in phenolic acids biosynthesis have been identified, but the underlying posttranslational regulatory mechanisms are poorly understood. Here, we demonstrated that the S. Read More

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Evaluation of a marker independent isolation method for circulating tumor cells in esophageal adenocarcinoma.

PLoS One 2021 7;16(5):e0251052. Epub 2021 May 7.

Department of Gastro-Intestinal Surgery, University Hospital Ghent, Ghent, Belgium.

Objective: The enrichment of circulating tumor cells (CTCs) from blood provides a minimally invasive method for biomarker discovery in cancer. Longitudinal interrogation allows monitoring or prediction of therapy response, detection of minimal residual disease or progression, and determination of prognosis. Despite inherent phenotypic heterogeneity and differences in cell surface marker expression, most CTC isolation technologies typically use positive selection. Read More

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Dual-Fluorescence Labeling Pseudovirus for Real-Time Imaging of Single SARS-CoV-2 Entry in Respiratory Epithelial Cells.

ACS Appl Mater Interfaces 2021 May 7. Epub 2021 May 7.

CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.

The pseudovirus strategy makes studies of highly pathogenic viruses feasible without the restriction of high-level biosafety facility, thus greatly contributing to virology and is used in the research studies of SARS-CoV-2. Here, we generated a dual-color pseudo-SARS-CoV-2 virus using a human immunodeficiency virus-1 pseudovirus production system and the SARS-CoV-2 spike (S) glycoprotein, of which the membrane was labeled with a lipophilic dye (DiO) and the genomic RNA-related viral protein R (Vpr) of the viral core was fused with mCherry. With this dual-color labeling strategy, not only the movement of the whole virus but also the fate of the labeled components can be traced. Read More

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Anti-hypersensitivity effect of betanin (red beetroot extract) via modulation of microglial activation in a mouse model of neuropathic pain.

Eur J Pain 2021 May 7. Epub 2021 May 7.

Department of Functional Anatomy, Graduate School of Medical Science, Kanazawa University, Japan.

Background: Neuropathic pain (NeP) medications have several side effects that affect NeP patients' quality of life. Betanin, the most common betacyanin pigment, has been shown to have potent antioxidant and anti-inflammatory properties in vivo; thus, it has potential as a healthcare treatment. In this study, we focused on betanin (red beetroot extract) as a potential therapy for NeP. Read More

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CD9-positive cells in the intermediate lobe of the pituitary gland are important supplier for prolactin-producing cells in the anterior lobe.

Cell Tissue Res 2021 May 7. Epub 2021 May 7.

Laboratory of Anatomy and Cell Biology, Department of Health Sciences, Kyorin University, 5-4-1 Shimorenjaku, Mitaka, Tokyo, 181-8612, Japan.

A supply of hormone-producing cells from stem/progenitor cells is critical to sustain the endocrine activity of the pituitary gland. In the adenohypophysis composing the anterior and intermediate lobe (AL and IL, respectively), stem/progenitor cells expressing sex-determining region Y-box 2 (SOX2) and S100β are located in the marginal cell layer (MCL) facing Rathke's cleft (primary niche) and the parenchyma of the AL (secondary niche). Our previous studies using mice and rats indicated that the tetraspanin superfamily CD9 and CD81 are expressed in S100β/SOX2-positive cells of primary and secondary niches (named CD9/CD81/S100β/SOX2-positive cell), and the cells located in the AL-side niches exhibit plasticity and multipotency. Read More

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IL-21 Is Associated With Virological Relapse of HBeAg Positive Chronic Hepatitis B After Discontinuance of Entecavir.

Turk J Gastroenterol 2021 Feb;32(2):178-186

Liver Disease Department, The Wuxi Fifth Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu Province, China.

Background: To investigate the association between interleukin-21 (IL-21) expression level and virological relapse (VR) of HBeAg positive chronic hepatitis B (CHB) after discontinuance of entecavir (ETV).

Methods: The serum IL-21 level of 112 CHB patients was measured at 0, 12, 24, 52, and 104 weeks after ETV discontinuance. ELISA was used for the measurement of serum IL-21 level. Read More

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February 2021

Persistent effects of pair bonding in lung cancer cell growth in monogamous .

Elife 2021 May 7;10. Epub 2021 May 7.

Department of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South Carolina, Columbia, United States.

Epidemiological evidence suggests that social interactions and especially bonding between couples influence tumorigenesis, yet whether this is due to lifestyle changes, homogamy (likelihood of individuals to marry people of similar health), or directly associated with host-induced effects in tumors remains debatable. In the present study, we explored if tumorigenesis is associated with the bonding experience in monogamous rodents at which disruption of pair bonds is linked to anxiety and stress. Comparison of lung cancer cell spheroids that formed in the presence of sera from bonded and bond-disrupted deer mice showed that in monogamous and , but not in polygamous , the disruption of pair bonds altered the size and morphology of spheroids in a manner that is consistent with the acquisition of increased oncogenic potential. Read More

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Integrated microenvironment-associated genomic profiles identify LRRC15 mediating recurrent glioblastoma-associated macrophages infiltration.

J Cell Mol Med 2021 May 7. Epub 2021 May 7.

Department of Pharmacology, School of Pharmacy, China Medical University, Shenyang, China.

Glioblastoma (GBM) is the most common malignant intracranial tumour with intrinsic infiltrative characteristics, which could lead to most patients eventually relapse. The prognosis of recurrent GBM patients remains unsatisfactory. Cancer cell infiltration and their interaction with the tumour microenvironment (TME) could promote tumour recurrence and treatment resistance. Read More

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RAS-association domain family 1A regulates the abnormal cell proliferation in psoriasis via inhibition of Yes-associated protein.

J Cell Mol Med 2021 May 7. Epub 2021 May 7.

State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.

Psoriasis is a chronic, inflammatory skin disease with a high incidence and recurrence; however, its exact pathogenesis and aetiology remain unclear. This study aimed to analyse the effect of the upstream negative regulator RAS-association domain family 1A (RASSF1A) on Yes-associated protein (YAP) in psoriasis. Skin lesions of 22 patients with psoriasis and 19 healthy controls were used. Read More

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Meflin defines mesenchymal stem cells and/or their early progenitors with multilineage differentiation capacity.

Genes Cells 2021 May 7. Epub 2021 May 7.

Department of Pathology, Nagoya University Graduate School of Medicine, Nagoya, 466-8550, Japan.

Mesenchymal stem cells (MSCs) are the likely precursors of multiple lines of mesenchymal cells. The existence of bona fide MSCs with self-renewal capacity and differentiation potential into all mesenchymal lineages, however, has been unclear because of the lack of MSC-specific marker(s) that are not expressed by the terminally differentiated progeny. Meflin, a glycosylphosphatidylinositol-anchored protein, is an MSC marker candidate that is specifically expressed in rare stromal cells in all tissues. Read More

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Transcriptomic insights into the zinc homeostasis of MCF-7 breast cancer cells via next-generation RNA sequencing.

Metallomics 2021 May 7. Epub 2021 May 7.

School of Science, Western Sydney University, Locked Bag 1797, Penrith, NSW 2751, Australia.

A significant gap in the knowledge of zinc homeostasis exists for breast cancer cells. In this study, we investigated the transcriptomic response of the luminal breast cancer cells (MCF-7) to the exposure of extracellular zinc using next-generation RNA sequencing (RNA-seq). The dataset was collected for three time points (T0, T30 and T120) in the time course of zinc treatment, which revealed the dramatic increase, up to 869-fold, of the gene expression for metallothioneins (MT1B, MT1F, MT1X and MT2A) and the zinc exporter ZnT1 (SLC30A1) at T30, continuingly through to T120. Read More

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Simple, efficient and open-source CRISPR/Cas9 strategy for multi-site genome editing in Populus tremula × alba.

Tree Physiol 2021 May 7. Epub 2021 May 7.

School of Forest Resources and Conservation, University of Florida, Gainesville, FL 32611, USA.

Although the CRISPR/Cas9 system has been successfully used for crop breeding, its application remains limited in forest trees. Here we describe an efficient gene editing strategy for hybrid poplar, based on the Golden Gate MoClo cloning. To test the system efficiency for generating single gene mutants, two sgRNAs were designed and incorporated into the MoClo Tool Kit level 2 binary vector with the Cas9 expression cassette to mutate SHORT ROOT (SHR) gene. Read More

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Relationship between hepatocellular carcinoma and depression via online database analysis.

Bioengineered 2021 Dec;12(1):1689-1697

Clinical laboratory, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.

There may be a mutually reinforcing relationship between hepatocellular carcinoma (HCC) and depression, but the mechanism is unknown. This study used bioinformatics to evaluate the relationship between HCC and depression at the genetic level. Genes associated with HCC and depression were obtained from pubmed2ensemble. Read More

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December 2021

Up-regulation of KLF17 expression increases the sensitivity of gastric cancer to 5-fluorouracil.

Int J Immunopathol Pharmacol 2021 Jan-Dec;35:20587384211010925

Department of General Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.

It has been reported that the expression of Krüppel-like factor 17 (KLF17) was associated with the occurrence, development, invasion, metastasis and chemotherapy resistance of various tumors. However, the detailed mechanisms by which KLF17 promotes chemotherapy resistance in gastric cancer (GC) have not been fully investigated. In the present study, we collected the GC tissues and non-tumor tissues (matched adjacent normal tissues with corresponding GC tissues) of 60 GC patients, used qRT-PCR, Western blot and immunohistochemistry assay to analyze the relationship between the expression of KLF17 and the clinical pathological data of the patients. Read More

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Potential biomarkers of infertility associated with microbiome imbalances.

Am J Reprod Immunol 2021 May 6:e13438. Epub 2021 May 6.

Microgenesis Co, US.

Problem: The aim of this study was to investigate the possible relationship between vaginal/rectal microbiome disbalances and miRNA expression with infertility.

Method Of Study: Observational, exploratory, preliminary study. A total of 287 multiple IVF failure infertile patients were recruited. Read More

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Xenotransplantation of neonatal porcine bone marrow-derived mesenchymal stem cells improves murine hind limb ischemia through lymphangiogenesis and angiogenesis.

Xenotransplantation 2021 May 6:e12693. Epub 2021 May 6.

Department of Regenerative Medicine & Transplantation, Faculty of Medicine, Fukuoka University, Fukuoka, Japan.

Background: The clinical utility of stem cell therapy for peripheral artery disease has not been fully discussed, and one obstacle is limited donor supplies. In this study, we attempted to rescue mouse ischemic hind limb by xenotransplantation of neonatal porcine bone marrow-derived mesenchymal stem cells (npBM-MSCs).

Methods: Neonatal porcine bone marrow-derived mesenchymal stem cells were transplanted to ischemic hind limbs of male C57BL/6J mice (npBM-MSCs group). Read More

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Preservation of endoplasmic reticulum (ER) Ca stores by deletion of inositol-1,4,5-trisphosphate receptor type 1 promotes ER retrotranslocation, proteostasis, and protein outer segment localization in cyclic nucleotide-gated channel-deficient cone photoreceptors.

FASEB J 2021 Jun;35(6):e21579

Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.

Endoplasmic reticulum (ER) Ca homeostasis relies on an appropriate balance between efflux- and influx-channel activity responding to dynamic changes of intracellular Ca levels. Dysregulation of this complex signaling network has been shown to contribute to neuronal and photoreceptor death in neuro- and retinal degenerative diseases, respectively. In mice with cone cyclic nucleotide-gated (CNG) channel deficiency, a model of achromatopsia/cone dystrophy, cones display early-onset ER stress-associated apoptosis and protein mislocalization. Read More

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Mycobacterium tuberculosis effector PPE36 attenuates host cytokine storm damage via inhibiting macrophage M1 polarization.

J Cell Physiol 2021 May 7. Epub 2021 May 7.

State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Institute of Modern Biopharmaceuticals, Southwest University, Chongqing, China.

Tuberculosis caused by Mycobacterium tuberculosis remains a serious global public health threat. Macrophage polarization is crucial for the innate immunity against M. tuberculosis. Read More

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BRET analysis reveals interaction between the lysophosphatidic acid receptor LPA2 and the lysophosphatidylinositol receptor GPR55 in live cells.

FEBS Lett 2021 May 6. Epub 2021 May 6.

Department of Life Science, Kyonggi University, Suwon, 16227, Republic of Korea.

Lysophosphatidic acid (LPA) and lysophosphatidylinositol bind to the G protein-coupled receptors (GPCRs) LPA and GPR55, respectively. LPA , a type 2 LPA receptor, and GPR55 are highly expressed in colon cancer and involved in cancer progression. However, crosstalk between the two receptors and potential effects on cellular physiology are not fully understood. Read More

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Transcriptional profiling of Microtus fortis responses to S. japonicum: New sight into Mf-Hsp90α resistance mechanism.

Parasite Immunol 2021 May 7:e12842. Epub 2021 May 7.

Molecular Biology Research Center, School of Life Science, Central South University, 110 Xiangya Road, Changsha, Hunan, 410078, People' s Republic of China.

Aims: Schistosomiasis is a parasitic disease with a chronic debilitating character caused by parasitic flatworms of the genus Schistosoma. The main disease-causing species of Schistosoma in China is S. japonicum. Read More

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Helios serves as a suppression marker to reduce regulatory T cell function in pancreatic cancer patients.

Immunol Res 2021 May 6. Epub 2021 May 6.

Department of Gastrointestinal Surgery, Shanghai Tenth People's Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.

Destabilizing and reprogramming regulatory T (Treg) cells have become a potential strategy to treat tumor. Mounting evidence indicates that the transcription factor Helios is required for the stable differentiation of Treg lineage. Hence, we investigated whether Helios suppression could be a potential treatment option for pancreatic cancer patients. Read More

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