128,428 results match your criteria expressed wild

Attenuated β-adrenergic response in calcium/calmodulin-dependent protein kinase IV-knockout mice.

PLoS One 2021 15;16(4):e0249932. Epub 2021 Apr 15.

Department of Anatomy, Kitasato University School of Medicine, Sagamihara, Japan.

In the present study, we examined the importance of Ca2+/calmodulin-dependent protein kinase IV (CaMKIV) in the regulation of cardiac function using genetically modified CaMKIV-null mice. RT-PCR analysis revealed decreased expression of voltage-dependent calcium channels in the cardiac myocytes of CaMKIV-null mice compared with wild-type mice. CaMKIV-null mice showed shortened QT time on electrocardiograms. Read More

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Novel missense mutations in PTCHD1 alter its plasma membrane subcellular localization and cause intellectual disability and autism spectrum disorder.

Hum Mutat 2021 Apr 15. Epub 2021 Apr 15.

UMR1253, iBrain, University of Tours, Inserm, Tours, France.

The X-linked PTCHD1 gene, encoding a synaptic membrane protein, has been involved in neurodevelopmental disorders with the description of deleterious genomic microdeletions or truncating coding mutations. Missense variants were also identified, however without any functional evidence supporting their pathogenicity level. We investigated 13 missense variants of PTCHD1, including eight previously described (c. Read More

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Growth and fecundity of colonizing hybrid Raphanus populations are environmentally dependent.

Am J Bot 2021 Apr 14. Epub 2021 Apr 14.

Department of Chemistry and Biology, Ryerson University, Toronto, Ontario, M5B 2K3, Canada.

Premise: Hybrid gene pools harbor more genetic variation than progenitor populations. Thus, we expect hybrid populations to exhibit more dynamic evolutionary responses to environmental variation. We ask how environmental variation experienced by adapted and transplanted populations influence the success of late-generation hybrid populations during invasion. Read More

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Silencing reduces wax accumulation in cotton leaves and leads to increased susceptibility to drought stress.

Plant Direct 2021 Apr 7;5(4):e00313. Epub 2021 Apr 7.

Key Laboratory of Oasis Eco-agriculture College of Agriculture Shihezi University Shihezi Xinjiang China.

Fatty acyl-CoA reductases (FAR) are involved in plant wax synthesis and play important roles in plant growth and development. However, little information is available for cotton genes. In this study, we carried out a genome-wide identification of genes in Upland cotton ( L. Read More

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Integrative analysis identifies an immune-relevant epigenetic signature for prognostication of non-G-CIMP glioblastomas.

Oncoimmunology 2021 Mar 29;10(1):1902071. Epub 2021 Mar 29.

Department of Stomatology, Xi'an No.3 Hospital, the Affiliated Hospital of Northwest University, Xi'an, Shaanxi Province, The People's Republic of China.

The clinical and molecular implications of DNA methylation alterations remain unclear among the majority of glioblastomas (GBMs) without glioma-CpGs island methylator phenotype (G-CIMP); integrative multi-level molecular profiling may provide useful information. Independent cohorts of non-G-CIMP GBMs or wild type (wt) lower-grade gliomas (LGGs) from local and public databases with DNA methylation and gene expression microarray data were included for discovery and validation of a multimarker signature, combined using a RISK score model. Bioinformatic and functional analyses were employed for biological validation. Read More

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Identification of amino acid residues required for inhibition of host gene expression by influenza A/Viet Nam/1203/2004 H5N1 PA-X.

J Virol 2021 Apr 14. Epub 2021 Apr 14.

Center for Animal Health Research, INIA-CISA, 28130 Valdeolmos, Madrid, Spain.

PA-X is a non-structural protein of influenza A virus (IAV), which is encoded by the polymerase acidic (PA) N-terminal region that contains a C-terminal +1 frameshifted sequence. IAV PA-X protein modulates virus-induced host innate immune responses and viral pathogenicity via suppression of host gene expression or cellular shutoff, through cellular mRNA cleavage. Highly pathogenic avian influenza viruses (HPAIV) of the H5N1 subtype naturally infect different avian species, they have an enormous economic impact in the poultry farming, and they also have zoonotic and pandemic potential, representing a risk to human public health. Read More

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Novel targetable FGFR2 and FGFR3 alterations in glioblastoma associate with aggressive phenotype and distinct gene expression programs.

Acta Neuropathol Commun 2021 Apr 14;9(1):69. Epub 2021 Apr 14.

Department of Neurosurgery, Rutgers-Robert Wood Johnson Medical School & University Hospital, Rutgers-New Jersey Medical School, New Brunswick, NJ, 08901, USA.

Prognostic molecular subgrouping of glioblastoma is an ongoing effort and the current classification includes IDH-wild-type and IDH-mutant entities, the latter showing significantly better prognosis. We performed a comparative integrated analysis of the FGFR glioblastoma subgroup consisting of 5 cases from a prospective 101-patient-cohort. FGFR alterations included FGFR2-TACC2 and FGFR2 amplifications arising in a multifocal IDH-mutant glioblastoma with unexpected 2. Read More

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A naturally occurring mutation in ATP synthase subunit c is associated with increased damage following hypoxia/reoxygenation in STEMI patients.

Cell Rep 2021 Apr;35(2):108983

Maria Cecilia Hospital, GVM Care & Research, 48033 Cotignola, Ravenna, Italy; Department of Medical Sciences, Section of Experimental Medicine, Laboratory for Technologies of Advanced Therapies (LTTA), University of Ferrara, 44121 Ferrara, Italy. Electronic address:

Preclinical models of ischemia/reperfusion injury (RI) demonstrate the deleterious effects of permeability transition pore complex (PTPC) opening in the first minutes upon revascularization of the occluded vessel. The ATP synthase c subunit (Csub) influences PTPC activity in cells, thus impacting tissue injury. A conserved glycine-rich domain in Csub is classified as critical because, when mutated, it modifies ATP synthase properties, protein interaction with the mitochondrial calcium (Ca) uniporter complex, and the conductance of the PTPC. Read More

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Engrailed-2 is a cell autonomous regulator of neurogenesis in cultured hippocampal neural stem cells.

Dev Neurobiol 2021 Apr 14. Epub 2021 Apr 14.

School of Graduate Studies, Rutgers -The State University of New Jersey, Piscataway, NJ, USA.

Abnormalities in genes that regulate early brain development are known risk factors for neurodevelopmental disorders. Engrailed-2 (En2) is a homeodomain transcription factor with established roles in cerebellar patterning. En2 is highly expressed in the developing mid-hindbrain region and En2 knockout (KO) mice exhibit major deficits in mid-hindbrain structures. Read More

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Osteoblast lineage cells and periodontal ligament fibroblasts regulate orthodontic tooth movement that is dependent on Nuclear Factor-kappa B (NF-kB) activation.

Angle Orthod 2021 Apr 14. Epub 2021 Apr 14.

Objectives: To investigate the role of NF-κB in osteoblast lineage cells and periodontal ligament (PDL) fibroblasts during orthodontic tooth movement (OTM).

Materials And Methods: Transgenic mice that expressed a dominant negative mutant of the inhibitor of kB kinase (IKK-DN) with lineage specific expression in osteoblastic cells and PDL fibroblasts driven by a response element in the collagen1α1 promoter and matched wild-type (WT) mice were examined. A 10-12 g force was applied by a NiTi coil and maintained for 5 or 12 days. Read More

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Effects of CcpA against salt stress in Lactiplantibacillus plantarum as assessed by comparative transcriptional analysis.

Appl Microbiol Biotechnol 2021 Apr 14. Epub 2021 Apr 14.

School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai, People's Republic of China.

Lactiplantibacillus plantarum is frequently exposed to salt stress during industrial applications. Catabolite control protein (CcpA) controls the transcription of many genes, but its role in the response to salt stress remains unclear. In this study, we used transcriptome analyses to investigate differences in the logarithmic growth phases of Lactiplantibacillus plantarum ST-III and its ccpA-knockout mutant when grown with or without salt and glycine betaine (GB). Read More

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Hepatic fibroblast growth factor 21 is involved in mediating functions of liraglutide in mice with dietary challenge.

Hepatology 2021 Apr 13. Epub 2021 Apr 13.

Div. of Advanced Diagnostics, Toronto General Hospital Research Institute, University Health Network, Canada.

Background And Aims: Several studies have shown that expression of hepatic fibroblast growth factor 21 (FGF21) can be stimulated by GLP-1-based diabetes drugs. As GLP-1 receptor (GLP-1R) is unlikely expressed in hepatocytes, we aimed to compare such stimulation in mice and in mouse-hepatocytes; determine the involvement of GLP-1R, and clarify whether FGF21 mediates certain functions of the GLP-1R agonist liraglutide.

Approaches And Results: Liver FGF21 expression was assessed in mice with daily liraglutide injection for 3 days, or in mouse primary hepatocytes (MPH) with direct liraglutide treatment. Read More

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Deletion of ER-retention Motif on SARS-CoV-2 Spike Protein Reduces Cell Hybrid During Cell-cell Fusion.

Res Sq 2021 Apr 9. Epub 2021 Apr 9.

The novel SARS-CoV-2 has quickly become a global pandemic since the first reported case in December 2019, with the virus infecting millions of people to date. The spike (S) protein of the SARS-CoV-2 virus plays a key role in binding to angiotensin-converting enzyme 2 (ACE2), a host cell receptor for SARS-CoV-2. S proteins that are expressed on the cell membrane can initiate receptor-dependent syncytia formation that is associated with extensive tissue damage. Read More

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Voluntary wheel running complements microdystrophin gene therapy to improve muscle function in mdx mice.

Mol Ther Methods Clin Dev 2021 Jun 3;21:144-160. Epub 2021 Mar 3.

Department of Human Nutrition, Foods, and Exercise and Metabolism Core, Virginia Tech, Blacksburg, VA 24060, USA.

We tested the hypothesis that voluntary wheel running would complement microdystrophin gene therapy to improve muscle function in young mdx mice, a model of Duchenne muscular dystrophy. mdx mice injected with a single dose of AAV9-CK8-microdystrophin or vehicle at age 7 weeks were assigned to three groups: mdxRGT (run, gene therapy), mdxGT (no run, gene therapy), or mdx (no run, no gene therapy). Wild-type (WT) mice were assigned to WTR (run) and WT (no run) groups. Read More

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miR-143 is implicated in growth plate injury by targeting IHH in precartilaginous stem cells.

Int J Med Sci 2021 3;18(9):1999-2007. Epub 2021 Mar 3.

Department of Orthopaedics, Children's Hospital of Soochow University, Suzhou, 215000, China.

Precartilaginous stem cells (PCSCs) are able to initiate chondrocyte and bone development. The present study aimed to investigate the role of and the underlying mechanisms involved in PCSC proliferation. In a rat growth plate injury model, tissue from the injury site was collected and the expression of and its potential targets was determined. Read More

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Mutation of cysteine residues increases heterologous expression of peach expansin in the methylotrophic yeast .

Plant Biotechnol (Tokyo) 2020 Dec;37(4):397-403

Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

The study of Carbohydrate-Active enZymes (CAZymes) associated with plant cell wall metabolism is important for elucidating the developmental mechanisms of plants and also for the utilization of plants as a biomass resource. The use of recombinant proteins is common in this context, but heterologous expression of plant proteins is particularly difficult, in part because the presence of many cysteine residues promotes denaturation, aggregation and/or protein misfolding. In this study, we evaluated two phenotypes of methylotrophic yeast as expression hosts for expansin from peach ( (L. Read More

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December 2020

Non-functional pancreatic neuroendocrine tumours: ATRX/DAXX and alternative lengthening of telomeres (ALT) are prognostically independent from ARX/PDX1 expression and tumour size.

Gut 2021 Apr 13. Epub 2021 Apr 13.

Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA, USA

Objective: Recent studies have found aristaless-related homeobox gene (ARX)/pancreatic and duodenal homeobox 1 (PDX1), alpha-thalassemia/mental retardation X-linked (ATRX)/death domain-associated protein (DAXX) and alternative lengthening of telomeres (ALT) to be promising prognostic biomarkers for non-functional pancreatic neuroendocrine tumours (NF-PanNETs). However, they have not been comprehensively evaluated, especially among small NF-PanNETs (≤2.0 cm). Read More

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[LIM-domain binding protein 2 regulated by mA modification inhibits lung adenocarcinoma cell proliferation ].

Nan Fang Yi Ke Da Xue Xue Bao 2021 Mar;41(3):329-335

Cancer Research Institute, Affiliated Cancer Hospital of Guangzhou Medical University//Guangzhou Key Laboratory of Translational Medicine on Cancer Treatment, Guangzhou 510095, China.

Objective: To investigate the role and expression pattern of LIM-domain binding protein 2 (LDB2) in lung adenocarcinoma.

Objective: We studied the expression pattern of LDB2 in lung adenocarcinoma based on data from the online databases TCGA, GEO and CPTAC, and the results were verified in lung adenocarcinoma tissues and cells using immunohistochemistry, qRT-PCR and Western blotting. The relationship between LDB2 and the prognosis of patients with lung adenocarcinoma was analyzed using GEPIA and GEO databases. Read More

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mRNA-Seq reveals the quorum sensing system luxS gene contributes to the environmental fitness of Streptococcus suis type 2.

BMC Microbiol 2021 Apr 13;21(1):111. Epub 2021 Apr 13.

College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China.

Background: Streptococcus suis type 2 (SS2) is an important zoonotic pathogen. We have previously reported the structure of LuxS protein and found that the luxS gene is closely related to biofilm, virulence gene expression and drug resistance of SS2. However, the mechanism of luxS mediated SS2 stress response is unclear. Read More

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Ageing genetic signature of hypersomatotropism.

Abdalla Elbialy

Open Biol 2021 Apr 14;11(4):200265. Epub 2021 Apr 14.

Laboratory of Fish Diseases, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Egypt.

Acromegaly is a pathological condition that is caused by over-secretion of growth hormone (GH) and develops primarily from a pituitary adenoma. Excess GH exposure over a prolonged period of time leads to a wide range of systemic manifestations and comorbidities. Studying the effect of excess GH on the cellular level could help to understand the underlying causes of acromegaly health complications and comorbidities. Read More

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Lack of collagen α6(IV) chain in mice does not cause severe-to-profound hearing loss or cochlear malformation, a distinct phenotype from nonsyndromic hearing loss with COL4A6 missense mutation.

PLoS One 2021 13;16(4):e0249909. Epub 2021 Apr 13.

Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.

Congenital hearing loss affects 1 in every 1000 births, with genetic mutations contributing to more than 50% of all cases. X-linked nonsyndromic hereditary hearing loss is associated with six loci (DFNX1-6) and five genes. Recently, the missense mutation (c. Read More

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Is YouTube promoting the exotic pet trade? Analysis of the global public perception of popular YouTube videos featuring threatened exotic animals.

PLoS One 2021 13;16(4):e0235451. Epub 2021 Apr 13.

School of Animal and Veterinary Sciences, The University of Adelaide, Adelaide, South Australia, Australia.

The exploitation of threatened exotic species via social media challenges efforts to regulate the exotic pet trade and consequently threatens species conservation. To investigate how such content is perceived by the global community, mixed model sentiment analysis techniques were employed to explore variations in attitudes expressed through text and emoji usage in public comments associated with 346 popular YouTube® videos starring exotic wild cats or primates in 'free handling' situations. Negative interactions between wild cats and primates with other species were found to be associated with both text and emoji median sentiment reduction, however were still accompanied by a median emoji sentiment above zero. Read More

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Genomic and transcriptomic insights into the habitat adaptation of the diazotrophic paddy-field cyanobacterium Nostoc sphaeroides.

Environ Microbiol 2021 Apr 13. Epub 2021 Apr 13.

School of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan, Hubei, 430079, China.

Nitrogen-fixing cyanobacteria are common in paddy fields, one of the most productive wetland ecosystems. Here, we present the complete genome of Nostoc sphaeroides, a paddy-field diazotroph used for food and medicine for more than 1700 years and deciphered the transcriptional regulation during the developmental transition from hormogonia to vegetative filaments with heterocysts. The genome of N. Read More

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Selective Suppression of Endogenous Gene Expression Using RNAi in Drosophila Schneider S2 Cells.

Methods Mol Biol 2021 ;2281:303-312

Department of Clinical Chemistry and Laboratory Medicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.

RNA interference (RNAi) is a posttranscriptional gene silencing method that is triggered by double-stranded RNA (dsRNA). RNAi is used to inactivate genes of interest and provides a genetic tool for loss-of-function studies in a variety of organisms.I have used this method to reveal the physiological roles of a number of endogenous proteins involved in mitochondrial DNA metabolism in Schneider cells, including the mitochondrial single-stranded DNA-binding protein. Read More

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January 2021

Molecular Insights into Myocilin and Its Glaucoma-Causing Misfolded Olfactomedin Domain Variants.

Acc Chem Res 2021 Apr 13. Epub 2021 Apr 13.

School of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Dr. NW, Atlanta, Georgia 30332-0400, United States.

ConspectusNumerous human disorders arise due to the inability of a particular protein to adopt its correct three-dimensional structure in the context of the cell, leading to aggregation. A new addition to the list of such protein conformational disorders is the inherited subtype of glaucoma. Different and rare coding mutations in myocilin, found in families throughout the world, are causal for early onset ocular hypertension, a key glaucoma risk factor. Read More

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Generation of a tissue-specific transgenic model for K8 phosphomutants: A tool to investigate the role of K8 phosphorylation during skin carcinogenesis in vivo.

Cell Biol Int 2021 Apr 13. Epub 2021 Apr 13.

Advanced Centre for Treatment, Research, and Education in Cancer, Navi Mumbai, 410210, India.

Keratin 8/18, the predominant keratin pair of simple epithelia, is known to be aberrantly expressed in several squamous cell carcinomas, where its expression is often correlated with increased invasion, neoplastic progression, and poor prognosis. The majority of keratin 8/18 structural and regulatory functions are governed by post-translational modifications, particularly phosphorylation. Apart from filament reorganization, cellular processes including cell cycle, cell growth, cellular stress, and apoptosis are known to be orchestrated by K8 phosphorylation at specific residues in the head and tail domains. Read More

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SARS‑CoV-2 RBD219-N1C1: A yeast-expressed SARS-CoV-2 recombinant receptor-binding domain candidate vaccine stimulates virus neutralizing antibodies and T-cell immunity in mice.

Hum Vaccin Immunother 2021 Apr 13:1-11. Epub 2021 Apr 13.

Texas Children's Hospital Center for Vaccine Development, Houston, TX, USA.

There is an urgent need for an accessible and low-cost COVID-19 vaccine suitable for low- and middle-income countries. Here, we report on the development of a SARS-CoV-2 receptor-binding domain (RBD) protein, expressed at high levels in yeast (), as a suitable vaccine candidate against COVID-19. After introducing two modifications into the wild-type RBD gene to reduce yeast-derived hyperglycosylation and improve stability during protein expression, we show that the recombinant protein, RBD219-N1C1, is equivalent to the wild-type RBD recombinant protein (RBD219-WT) in an ACE-2 binding assay. Read More

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Identification and functional characterization of two bamboo FD gene homologs having contrasting effects on shoot growth and flowering.

Sci Rep 2021 Apr 12;11(1):7849. Epub 2021 Apr 12.

Department of Life Sciences, Presidency University, Kolkata, India.

Bamboos, member of the family Poaceae, represent many interesting features with respect to their fast and extended vegetative growth, unusual, yet divergent flowering time across species, and impact of sudden, large scale flowering on forest ecology. However, not many studies have been conducted at the molecular level to characterize important genes that regulate vegetative and flowering habit in bamboo. In this study, two bamboo FD genes, BtFD1 and BtFD2, which are members of the florigen activation complex (FAC) have been identified by sequence and phylogenetic analyses. Read More

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High-salt diet downregulates TREM2 expression and blunts efferocytosis of macrophages after acute ischemic stroke.

J Neuroinflammation 2021 Apr 12;18(1):90. Epub 2021 Apr 12.

Department of Neurology, Mental and Neurological Disease Research Center, The Third Affiliated Hospital of Sun Yat-sen University, 600 Tianhe Road, Guangzhou, Guangdong, 510630, People's Republic of China.

Background: A high-salt diet (HSD) is one of the major risk factors for acute ischemic stroke (AIS). As a potential mechanism, surplus salt intake primes macrophages towards a proinflammatory phenotype. In this study, whether HSD could blunt the efferocytic capability of macrophages after ischemic stroke, thus exacerbating post-stroke neural inflammation, was investigated. Read More

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Characterization and transcriptomic analysis of a novel yellow-green leaf wucai (Brassica campestris L.) germplasm.

BMC Genomics 2021 Apr 12;22(1):258. Epub 2021 Apr 12.

College of Horticulture, Vegetable Genetics and Breeding Laboratory, Anhui Agricultural University, 130 West Changjiang Road, Hefei, 230036, Anhui, China.

Background: Leaf color mutants are the ideal materials to explore the pathways of chlorophyll (Chl) metabolism, chloroplast development, and photosynthesis system. In this study, a spontaneous yellow-green leaf wucai (Brassica campestris L.) mutant "WY16-13" was identified, which exhibited yellow-green leaf color during its entire growth period. Read More

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