19,323 results match your criteria expressed nicotiana

RNA-dependent RNA polymerase 1 delays the accumulation of viroids in infected plants.

Mol Plant Pathol 2021 Jul 23. Epub 2021 Jul 23.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

RNA-dependent RNA polymerase 1 (RDR1) is essential for plant antiviral defence, but its role in plant defence against viroid infection remains unknown. The present study aimed to identify the function and mechanism of RDR1 in plant resistance to viroid infection. Overexpression of Nicotiana tabacum RDR1 (NtRDR1) delayed the accumulation of potato spindle tuber viroid (PSTVd) genomic RNA and PSTVd-derived small RNA (sRNA) in Nicotiana benthamiana plants at the early invasion stage, but not in the late stage of infection. Read More

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Molecular characterization of a catalase gene from the green peach aphid (Myzus persicae).

Arch Insect Biochem Physiol 2021 Jul 26:e21835. Epub 2021 Jul 26.

Anhui Provincial Key Laboratory of Integrated Pest Management on Crops, School of Plant Protection, Anhui Agricultural University, Hefei, China.

The green peach aphid, Myzus persicae (Sulzer), is a serious agricultural pest with a worldwide distribution. Catalase (CAT), which is encoded by the catalase (Cat) gene, is an extremely important antioxidant enzyme that plays a pivotal role in protecting cells against the toxic effects of hydrogen peroxide. The Cat gene has not been characterized in M. Read More

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The Instability of Dimeric Fc-Fusions Expressed in Plants Can Be Solved by Monomeric Fc Technology.

Front Plant Sci 2021 9;12:671728. Epub 2021 Jul 9.

Department of Applied Genetics and Cell Biology, Institute for Plant Biotechnology and Cell Biology, University of Natural Resources and Life Sciences Vienna, Vienna, Austria.

The potential therapeutic value of many proteins is ultimately limited by their rapid clearance. One strategy to limit clearance by metabolism and excretion, and improving the stability of therapeutic proteins, is their fusion to the immunoglobulin fragment crystallizable region (Fc). The Fc region plays multiple roles in (i) dimerization for the formation of "Y"-shaped structure of Ig, (ii) Fc-mediated effector functions, (iii) extension of serum half-life, and (iv) a cost-effective purification tag. Read More

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Comparative Transcriptome Analysis of Salt Stress-Induced Leaf Senescence in .

Front Plant Sci 2021 9;12:666660. Epub 2021 Jul 9.

Grassland Agri-Husbandry Research Center, College of Grassland Science, Qingdao Agricultural University, Qingdao, China.

Leaves are the most critical portion of forage crops such as alfalfa (). Leaf senescence caused by environmental stresses significantly impacts the biomass and quality of forages. To understand the molecular mechanisms and identify the key regulator of the salt stress-induced leaf senescence process, we conducted a simple and effective salt stress-induced leaf senescence assay in , which was followed by RNA-Seq analysis coupled with physiological and biochemical characterization. Read More

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A cell wall-localized NLR confers resistance to Soybean mosaic virus by recognizing viral-encoded cylindrical inclusion protein.

Mol Plant 2021 Jul 21. Epub 2021 Jul 21.

National Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Soybean-Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China. Electronic address:

Soybean mosaic virus (SMV) causes severe yield losses and seed quality reduction in soybean (Glycine max) production worldwide. Rsc4 from cultivar Dabaima is a dominant genetic locus for SMV resistance, and its mapping interval contains three Nucleotide-binding domain Leucine-rich Repeat containing (NLR) candidates (Rsc4-1, Rsc4-2, and Rsc4-3). The NLR-type resistant proteins were considered as important intracellular pathogen sensors in the previous studies. Read More

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Transcriptomic analysis provides insights into the AUXIN RESPONSE FACTOR 6-mediated repression of nicotine biosynthesis in tobacco (Nicotiana tabacum L.).

Plant Mol Biol 2021 Jul 24. Epub 2021 Jul 24.

Yunnan Academy of Tobacco Agricultural Sciences, Kunming, 650021, Yunnan, China.

Key Message: NtARF6 overexpression represses nicotine biosynthesis in tobacco. Transcriptome analysis suggests that NtARF6 acts as a regulatory hub that connect different phytohormone signaling pathways to antagonize the jasmonic acid-induced nicotine biosynthesis. Plant specialized metabolic pathways are regulated by a plethora of molecular regulators that form complex networks. Read More

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The -Specific Truncated NLR Protein CbCN Enhances the Innate Immunity against .

Int J Mol Sci 2021 Jul 18;22(14). Epub 2021 Jul 18.

National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Korea.

Chili pepper () is an important fruit and spice used globally, but its yield is seriously threatened by anthracnose. is particularly valuable as it carries advantageous disease resistance genes. However, most of the genes remain to be identified. Read More

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Action of Multiple Rice β-Glucosidases on Abscisic Acid Glucose Ester.

Int J Mol Sci 2021 Jul 15;22(14). Epub 2021 Jul 15.

School of Chemistry, Institute of Science, Center for Biomolecular Structure, Function and Application, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.

Conjugation of phytohormones with glucose is a means of modulating their activities, which can be rapidly reversed by the action of β-glucosidases. Evaluation of previously characterized recombinant rice β-glucosidases found that nearly all could hydrolyze abscisic acid glucose ester (ABA-GE). Os4BGlu12 and Os4BGlu13, which are known to act on other phytohormones, had the highest activity. Read More

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Enhancement of Resistance Against Dehydration-Induced Leaf Senescence via Metabolite/Phytohormone-Gene Regulatory Networks Modulated by Melatonin.

Front Plant Sci 2021 6;12:686062. Epub 2021 Jul 6.

College of Tobacco Science, Henan Agricultural University, Zhengzhou, China.

Melatonin (MEL) is a pleiotropic agent with crucial functions reported in a variety of stress responses and developmental processes. Although MEL involvement in plant defense against natural leaf senescence has been widely reported, the precise regulatory mechanisms by which it delays stress-induced senescence remain unclear. In this study, we found that foliar spraying of melatonin markedly ameliorated dehydration-induced leaf senescence in , accompanied by attenuated oxidative damage, expression of senescence-related genes, and reduced endogenous ABA production. Read More

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The Pepper Mitogen-Activated Protein Kinase CaMAPK7 Acts as a Positive Regulator in Response to Infection.

Front Microbiol 2021 6;12:664926. Epub 2021 Jul 6.

Fujian Provincial Key Laboratory of Applied Genetics, Fujian Agriculture and Forestry University, Fuzhou, China.

Mitogen-activated protein kinase (MAPK) pathways play a vital role in multiple plant processes, including growth, development, and stress signaling, but their involvement in response to is poorly understood, particularly in pepper plants. Herein, was identified from the pepper genome and functionally analyzed. The accumulations of transcripts and promoter activities were both significantly induced in response to strain FJC100301 infection, and exogenously applied phytohormones, including methyl jasmonate (MeJA), brassinolide (BR), salicylic acid (SA), and ethephon (ETN), were decreased by abscisic acid (ABA) treatment. Read More

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Tobacco Smoking Increases Methylation of Polypyrimidine Tract Binding Protein 1 Promoter in Intracranial Aneurysms.

Front Aging Neurosci 2021 6;13:688179. Epub 2021 Jul 6.

Department of Neurosurgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

DNA methylation at the gene promoter region is reportedly involved in the development of intracranial aneurysm (IA). This study aims to investigate the methylation levels of polypyrimidine tract-binding protein 1 () in IA, as well as its potential to predict IA. Forty-eight patients with IA and 48 age- and sex-matched healthy controls were recruited into this study. Read More

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Genome-wide survey of the F-box/Kelch (FBK) members and molecular identification of a novel FBK gene TaAFR in wheat.

PLoS One 2021 22;16(7):e0250479. Epub 2021 Jul 22.

College of Life Sciences/Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, Hebei Agricultural University, Baoding, Hebei, China.

F-box proteins play critical roles in plant responses to biotic/abiotic stresses. In the present study, a total of 68 wheat F-box/Kelch (TaFBK) genes, unevenly distributed across 21 chromosomes and encoding 74 proteins, were identified in EnsemblPlants. Protein sequences were compared with those of Arabidopsis and three cereal species by phylogenetic and domain analyses, where the wheat sequences were resolved into 6 clades. Read More

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Purification and characterization of an amyloidogenic repeat domain from the functional amyloid Pmel17.

Protein Expr Purif 2021 Jul 20;187:105944. Epub 2021 Jul 20.

Laboratory of Protein Conformation and Dynamics, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, United States. Electronic address:

The pre-melanosomal protein (Pmel17) is a human functional amyloid that supports melanin biosynthesis within melanocytes. This occurs in the melanosome, a membrane-bound organelle with an acidic intraluminal pH. The repeat region of Pmel17 (RPT, residues 315-444) has been previously shown to form amyloid aggregates under acidic melanosomal conditions, but not under neutral cytosolic conditions, when expressed and purified using a C-terminal hexa-histidine tag (RPT-His). Read More

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Downregulation of Light-Harvesting Complex II Induces ROS-Mediated Defense Against Turnip Mosaic Virus Infection in .

Front Microbiol 2021 5;12:690988. Epub 2021 Jul 5.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, China.

The light-harvesting chlorophyll / complex protein 3 (LHCB3) of photosystem II plays important roles distributing the excitation energy and modulating the rate of state transition and stomatal response to abscisic acid. However, the functions of LHCB3 in plant immunity have not been well investigated. Here, we show that the expression of in () was down-regulated by turnip mosaic virus (TuMV) infection. Read More

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Roles for B[a]P and FICZ in subchondral bone metabolism and experimental temporomandibular joint osteoarthritis via the AhR/Cyp1a1 signaling axis.

Sci Rep 2021 Jul 21;11(1):14927. Epub 2021 Jul 21.

Department of Orthodontics, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8525, Japan.

Bone loss due to smoking represents a major risk factor for fractures and bone osteoporosis. Signaling through the aryl hydrocarbon receptor (AhR) and its ligands contributes to both bone homeostasis and inflammatory diseases. It remains unclear whether the same AhR signaling axis affects the temporomandibular joint (TMJ). Read More

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Transient Expression Assay in Plants Using .

Bio Protoc 2018 Jun 20;8(12):e2894. Epub 2018 Jun 20.

Shanghai Center for Plant Stress Biology/CAS Center for Excellence in Molecular Plant Science, Chinese Academy of Sciences, Shanghai, China.

-mediated transient expression has greatly contributed to research in molecular plant biology but has low efficiency and inconsistency in ). Here, we describe a simple, efficient and fast protocol to make transient gene expression in Arabidopsis plants using . This protocol has been successfully used to assess protein sub-cellular localization and accumulation, enzyme activity, and protein-protein interaction. Read More

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Impacts of continued exposure to a susceptible host genotype on aggressiveness of isolates adapted to multiple sources of partial resistance.

Plant Dis 2021 Jul 20. Epub 2021 Jul 20.

North Carolina State University, 6798, Entomology and Plant Pathology, Raleigh, North Carolina, United States;

Pathogen adaptation can threaten the durability of partial resistance. Mixed plantings of susceptible and partially resistant varieties may prolong the effectiveness of partial resistance, but little is known about how continued exposure to a susceptible genotype can change the aggressiveness of pathogen isolates adapted to a source of partial resistance. The objective of this study was to examine the effects of continued exposure to a highly susceptible tobacco genotype on isolates of that had been adapted to partial resistance. Read More

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Enantioselective bioaccumulation and detoxification mechanisms of earthworms (Eisenia fetida) exposed to mandipropamid.

Sci Total Environ 2021 Jul 15;796:149051. Epub 2021 Jul 15.

Tobacco Research Institute of Chinese Academy of Agricultural Sciences (CAAS), Qingdao 266101, PR China. Electronic address:

As a novel chiral amide fungicide, the enantioselective behaviors of mandipropamid in the soil environment are unclear. Furthermore, there is a need to understand the stress response mechanisms of soil organisms exposed to mandipropamid isomers. Therefore, the selective bioaccumulation of mandipropamid isomers and detoxification mechanisms of earthworms (Eisenia fetida) were investigated in this study. Read More

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Transcriptional regulation and functional analysis of Nicotiana tabacum under salt and ABA stress.

Biochem Biophys Res Commun 2021 Jul 16;570:110-116. Epub 2021 Jul 16.

Agricultural Big-Data Research Center and College of Plant Protection, Shandong Agricultural University, Taian, 271018, China. Electronic address:

Soil salinization is an important factor that restricts crop quality and yield and causes an enormous toll to human beings. Salt stress and abscisic acid (ABA) stress will occur in the process of soil salinization. In this study, transcriptome sequencing of tobacco leaves under salt and ABA stress in order to further study the resistance mechanism of tobacco. Read More

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Melatonin and Its Homologs Induce Immune Responses Receptors trP47363-trP13076 in .

Front Plant Sci 2021 30;12:691835. Epub 2021 Jun 30.

Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Department of Plant Pathology, College of Plant Protection, Ministry of Education, Nanjing Agricultural University, Nanjing, China.

Melatonin (N-acetyl-5-methoxytryptamine), a naturally occurring small molecule, can protect plants against abiotic stress after exogenous treatmenting with it. It is not known if melatonin homologs, such as 5-methoxytryptamine and 5-methoxyindole, that are easy and more cost-effective to synthesize can stimulate the plant immune system in the same manner as melatonin. In the present study, we assessed the biological activity of the melatonin homologs, 5-methoxytryptamin and 5-methoxyindole. Read More

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The GB4.0 Platform, an All-In-One Tool for CRISPR/Cas-Based Multiplex Genome Engineering in Plants.

Front Plant Sci 2021 1;12:689937. Epub 2021 Jul 1.

Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València, Valencia, Spain.

CRISPR/Cas ability to target several loci simultaneously (multiplexing) is a game-changer in plant breeding. Multiplexing not only accelerates trait pyramiding but also can unveil traits hidden by functional redundancy. Furthermore, multiplexing enhances dCas-based programmable gene expression and enables cascade-like gene regulation. Read More

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Endomembrane-Targeting Effectors Modulate PAMP Triggered Immune Responses in Plants.

Front Microbiol 2021 1;12:651279. Epub 2021 Jul 1.

Department of Biology, University of Saskatchewan, Saskatoon, SK, Canada.

is a devastating obligate, intracellular, biotrophic pathogen that causes clubroot disease in crucifer plants. Disease progression is regulated by effector proteins secreted by . Twelve putative effectors (PEs), expressed at various stages of disease development [0, 2, 5, 7, 14, 21, and 28 days post inoculation (DPI)] in Arabidopsis and localizing to the plant endomembrane system, were studied for their roles in pathogenesis. Read More

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Impact of 6-Month Exposure to Aerosols From Potential Modified Risk Tobacco Products Relative to Cigarette Smoke on the Rodent Gastrointestinal Tract.

Front Microbiol 2021 2;12:587745. Epub 2021 Jul 2.

PMI R&D, Philip Morris Products S.A., Neuchâtel, Switzerland.

Cigarette smoking causes adverse health effects that might occur shortly after smoking initiation and lead to the development of inflammation and cardiorespiratory disease. Emerging studies have demonstrated the role of the intestinal microbiome in disease pathogenesis. The intestinal microbiome is susceptible to the influence of environmental factors such as smoking, and recent studies have indicated microbiome changes in smokers. Read More

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Antibodies induced by oral immunization of mice with a recombinant protein produced in tobacco plants harboring epitopes.

Plant Cell Tissue Organ Cult 2021 Jul 10:1-12. Epub 2021 Jul 10.

División de Biología Molecular, IPICYT, Camino a la Presa San José 2055, 78216 San Luis Potosí, S.L.P. México.

causes whooping cough or pertussis, disease that has not been eradicated and is reemerging despite the availability and massive application for decades of vaccines, such as Boostrix® which is an acellular vaccine harboring two regions of S1 subunit of the pertussis toxin, one region of filamentous hemagglutinin and one region of pertactin. In 2008, the World Health Organization estimated 16 million new cases and 95% occurred in developing countries with 195,000 children's deaths. We attempt to improve the vaccine against whooping cough and reduce its production costs by obtaining plants and bacteria expressing a heterologous protein harboring pertactin, pertussis toxin, and filamentous hemagglutinin epitopes from and assessing its immunogenicity after oral administration to mice. Read More

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Effects of smoking on oral health: Awareness among dental patients and their attitude towards its cessation.

Indian J Dent Res 2021 Jan-Feb;32(1):23-26

Department of Public Health Dentistry, Coorg Institute of Dental Sciences, Karnataka, India.

Context: Tobacco smoking is the leading cause of preventable premature mortality in the world. Smoking is also detrimental to oral health, causing unaesthetic tooth staining, bad breath, periodontal diseases, impaired healing of wounds, increased risk of dental implant failure, precancerous conditions and oral cancer.

Aims: To assess the awareness of the effects of smoking on oral health among smokers in Mangalore city and to assess the smoker's willingness to quit smoking. Read More

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Haplotype-resolved genome assembly provides insights into evolutionary history of the tea plant Camellia sinensis.

Nat Genet 2021 Jul 15. Epub 2021 Jul 15.

Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Tea is an important global beverage crop and is largely clonally propagated. Despite previous studies on the species, its genetic and evolutionary history deserves further research. Here, we present a haplotype-resolved assembly of an Oolong tea cultivar, Tieguanyin. Read More

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Universal stress protein-like gene from mulberry enhances abiotic stress tolerance in Escherichia coli and transgenic tobacco cells.

Plant Biol (Stuttg) 2021 Jul 15. Epub 2021 Jul 15.

Department of Crop Physiology, University of Agricultural Sciences Bangalore, GKVK Campus, Bengaluru, India.

Universal stress proteins (USPs) are a conserved group of proteins initially identified and characterized in bacteria. USPs are induced under multiple stresses, and are important for stress acclimation. We cloned a USP-like gene designated as MaUSP1-like from mulberry and expressed in bacteria and tobacco to examine its relevance in abiotic stress tolerance. Read More

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Insights into heat response mechanisms in Clematis species: physiological analysis, expression profiles and function verification.

Plant Mol Biol 2021 Jul 14. Epub 2021 Jul 14.

Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China.

Key Message: Our results provide insights into heat response mechanisms among Clematis species. Overexpressing CvHSFA2 enhanced the heat resistance of yeast and silencing NbHSFA2 reduced the heat resistance of tobacco. Clematis species are commonly grown in western and Japanese gardens. Read More

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Geminiviruses encode additional small proteins with specific subcellular localizations and virulence function.

Nat Commun 2021 07 13;12(1):4278. Epub 2021 Jul 13.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

Geminiviruses are plant viruses with limited coding capacity. Geminivirus-encoded proteins are traditionally identified by applying a 10-kDa arbitrary threshold; however, it is increasingly clear that small proteins play relevant roles in biological systems, which calls for the reconsideration of this criterion. Here, we show that geminiviral genomes contain additional ORFs. Read More

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