1,117,313 results match your criteria expressed human

iNetModels 2.0: an interactive visualization and database of multi-omics data.

Nucleic Acids Res 2021 Apr 13. Epub 2021 Apr 13.

Science for Life Laboratory, KTH - Royal Institute of Technology, Stockholm SE-171 21, Sweden.

It is essential to reveal the associations between various omics data for a comprehensive understanding of the altered biological process in human wellness and disease. To date, very few studies have focused on collecting and exhibiting multi-omics associations in a single database. Here, we present iNetModels, an interactive database and visualization platform of Multi-Omics Biological Networks (MOBNs). Read More

View Article and Full-Text PDF

Genome-wide characterization of human minisatellite VNTRs: population-specific alleles and gene expression differences.

Nucleic Acids Res 2021 Apr 13. Epub 2021 Apr 13.

Graduate Program in Bioinformatics, Boston University, Boston, MA 02215, USA.

Variable Number Tandem Repeats (VNTRs) are tandem repeat (TR) loci that vary in copy number across a population. Using our program, VNTRseek, we analyzed human whole genome sequencing datasets from 2770 individuals in order to detect minisatellite VNTRs, i.e. Read More

View Article and Full-Text PDF

Differential DNA Methylation of the IMMP2L Gene in Families with Maternally Inherited 7q31.1 Microdeletions is Associated with Intellectual Disability and Developmental Delay.

Cytogenet Genome Res 2021 Apr 13:1-15. Epub 2021 Apr 13.

Research Institute of Medical Genetics, Tomsk National Research Medical Center, Tomsk, Russian Federation.

Most copy number variations (CNVs) in the human genome display incomplete penetrance with unknown underlying mechanisms. One such mechanism may be epigenetic modification, particularly DNA methylation. The IMMP2L gene is located in a critical region for autism susceptibility on chromosome 7q (AUTS1). Read More

View Article and Full-Text PDF

Dual activation of Hedgehog and Wnt/β-catenin signaling pathway caused by downregulation of SUFU targeted by miRNA-150 in human gastric cancer.

Aging (Albany NY) 2021 Apr 12;13. Epub 2021 Apr 12.

Guangdong Key Laboratory for Genome Stability and Disease Prevention, Department of Pathology, Shenzhen University School of Medicine, Shenzhen 518060, Guangdong, P.R. China.

Mounting evidence has shown that miRNA-150 expression is upregulated in gastric cancer (GC) and is associated with gastric carcinogenesis, but the underlying oncogenic mechanism remains elusive. Here, we discovered that miRNA-150 targets the tumor suppressor SUFU to promote cell proliferation, migration, and the epithelial-mesenchymal transition (EMT) via the dual activation of Hedgehog (Hh) and Wnt signaling. MiRNA-150 was highly expressed in GC tissues and cell lines, and the level of this miRNA was negatively related to that of SUFU. Read More

View Article and Full-Text PDF

Direct conversion of adult human fibroblasts into functional endothelial cells using defined factors.

Biomaterials 2021 Mar 24;272:120781. Epub 2021 Mar 24.

Division of Cardiology, Department of Internal Medicine, Seoul National University Hospital, Seoul, South Korea. Electronic address:

We aimed to directly convert adult human dermal fibroblasts (aHDFs) into functional endothelial cells (ECs). Lentiviral vectors encoding endothelial transcription factors (TFs) were constructed. We examined whether five TFs (FOXO1, ER71, KLF2, TAL1, and LMO2) used for the generation of mouse induced ECs (iECs) could convert the aHDFs into human iECs. Read More

View Article and Full-Text PDF

CD24 gene inhibition and TIMP-4 gene upregulation by Imperata cylindrica's root extract prevents metastasis of CaSki cells via inhibiting PI3K/Akt/snail signaling pathway and blocking EMT.

J Ethnopharmacol 2021 Apr 10:114111. Epub 2021 Apr 10.

National Centre for Biological Science - Tata Institute for Biological Research, Bangalore, India. Electronic address:

Ethnopharmacological Relevance: Imperata cylindrica (L.) Raeusch (Gramineae) is a medicinal spice traditionally used in the treatment of hypertension and cancer.

Aim Of The Study: To assess the anti-metastatic potential of the methanol extract of I. Read More

View Article and Full-Text PDF

Clinico-pathological and transcriptomic analysis of radiation-induced lung injury in non-human primates.

Int J Radiat Oncol Biol Phys 2021 Apr 10. Epub 2021 Apr 10.

Department of Pathology, Section on Comparative Medicine, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157-1040, USA. Electronic address:

Purpose: Radiation-induced lung injury (RILI) is a progressive condition, with an early phase, radiation pneumonitis (RP), and a late phase, lung fibrosis (LF). RILI may occur after partial-body ionizing radiation exposures or by internal radioisotope exposure, with wide individual variability in timing and extent of lung injury. This study aims to provide new insights into the pathogenesis and progression of RILI in the non-human primate (NHP) rhesus macaque model. Read More

View Article and Full-Text PDF

In-silico design of a multivalent epitope-based vaccine against Candida auris.

Microb Pathog 2021 Apr 10:104879. Epub 2021 Apr 10.

Department of Molecular Biology and Genetic Engineering, India. Electronic address:

Candida auris is a rapidly emerging human pathogenic fungus with a high mortality rate. A recent report suggests that the new clinical isolates are showing resistance to the major classes of antifungal drugs. Due to the emergence of drug resistance, it becomes imperative to seek novel therapies for the treatment of C. Read More

View Article and Full-Text PDF

The brominated flame retardants TBECH and DPTE alter prostate growth, histology and gene expression patterns in the mouse.

Reprod Toxicol 2021 Apr 10. Epub 2021 Apr 10.

Biology, The Life Science Center, School of Science and Technology, Örebro University, SE-701 82, Örebro, Sweden. Electronic address:

The brominated flame retardants (BFRs), 1,2-dibromo-4-(1,2 dibromoethyl)cyclohexane (TBECH) and 2,3-dibromopropyl-2,4,6-tribromophenyl ether (DPTE) bind to the androgen receptor (AR). In vitro bioassays have shown that TBECH is a potent androgen agonist while DPTE is a potent AR antagonist. Both TBECH and DPTE alter gene expression associated with AR regulation. Read More

View Article and Full-Text PDF

Synergistic effects of green tea extract and Paclitaxel in the induction of mitochondrial apoptosis in ovarian cancer cell lines.

Gene 2021 Apr 10:145638. Epub 2021 Apr 10.

Department of Medical Radiation, Faculty of Engineering, Islamic Azad University, Science and Research Branch, Tehran, Iran. Electronic address:

Background: Green tea is a natural compound with anti-neoplastic properties. Paclitaxel (PTX) is a natural anti-tumor medication used to manage patients with advanced ovarian cancer. This manuscript evaluated the cytotoxic effects of green tea extract combined with PTX drug in two human ovarian cancer cell lines (p53-negative cell line, SKOV-3; and mutant type p53 cell line, OVCAR-3) and underlying mechanisms. Read More

View Article and Full-Text PDF

Triacylglycerol lipidome from human meibomian gland epithelial cells: Description, response to culture conditions, and perspective on function.

Exp Eye Res 2021 Apr 10:108573. Epub 2021 Apr 10.

University of Alabama at Birmingham, School of Optometry, Department of Optometry and Vision Science, Birmingham, AL, USA.

Preliminary work has shown that select triacylglycerols (TAGs) are upregulated in a preclinical model of MGD, suggesting that TAGs may be an important outcome variable in research involving human meibomian gland epithelial cells (HMGECs). The purpose of this study was to explore the HMGEC TAG lipidome in culture conditions known to influence differentiation. HMGECs were differentiated in DMEM/F12 with 10 ng/ml EGF, FBS (2% or 10%), and rosiglitazone (0, 20, or 50 μM) for two or five days. Read More

View Article and Full-Text PDF

Secreted Extracellular Cyclophilin A is a Novel Mediator of Ventilator Induced Lung Injury.

Am J Respir Crit Care Med 2021 Apr 13. Epub 2021 Apr 13.

Imperial College London, Anaesthetics & Intensive Care, London, United Kingdom of Great Britain and Northern Ireland;

Rationale: Mechanical ventilation is a mainstay of intensive care but contributes to the mortality of patients through ventilator induced lung injury. Extracellular Cyclophilin A is an emerging inflammatory mediator and metalloproteinase inducer, and the gene responsible for its expression has recently been linked to COVID-19 infection.

Objectives: Here we explore the involvement of extracellular Cyclophilin A in the pathophysiology of ventilator-induced lung injury. Read More

View Article and Full-Text PDF

Lack of collagen α6(IV) chain in mice does not cause severe-to-profound hearing loss or cochlear malformation, a distinct phenotype from nonsyndromic hearing loss with COL4A6 missense mutation.

PLoS One 2021 13;16(4):e0249909. Epub 2021 Apr 13.

Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.

Congenital hearing loss affects 1 in every 1000 births, with genetic mutations contributing to more than 50% of all cases. X-linked nonsyndromic hereditary hearing loss is associated with six loci (DFNX1-6) and five genes. Recently, the missense mutation (c. Read More

View Article and Full-Text PDF

Coordinated changes in the expression of Wnt pathway genes following human and rat peripheral nerve injury.

PLoS One 2021 13;16(4):e0249748. Epub 2021 Apr 13.

Laboratory for Neuroregeneration, Netherlands Institute for Neuroscience, An Institute of the Royal Academy of Arts and Sciences, Amsterdam, The Netherlands.

A human neuroma-in continuity (NIC), formed following a peripheral nerve lesion, impedes functional recovery. The molecular mechanisms that underlie the formation of a NIC are poorly understood. Here we show that the expression of multiple genes of the Wnt family, including Wnt5a, is changed in NIC tissue from patients that underwent reconstructive surgery. Read More

View Article and Full-Text PDF

Base editing repairs an SGCA mutation in human primary muscle stem cells.

JCI Insight 2021 Apr 13. Epub 2021 Apr 13.

Muscle Research Unit, Charité University Hospital Berlin, Berlin, Germany.

Skeletal muscle can regenerate from muscle stem cells and their myogenic precursor cell progeny, myoblasts. However, precise gene editing in human muscle stem cells for autologous cell replacement therapies of untreatable genetic muscle diseases has not yet been reported. Loss-of-function mutations in SGCA, encoding α-sarcoglycan, cause limb-girdle muscular dystrophy 2D/R3, an early onset, severe and rapidly progressive form of muscular dystrophy affecting equally girls and boys. Read More

View Article and Full-Text PDF

Challenges and Considerations for Reducing Diabetes Distress and Fear of Hypoglycemia in Parents of Youth with Type 1 Diabetes During COVID-19.

JMIR Pediatr Parent 2021 Apr 9. Epub 2021 Apr 9.

Center for Healthcare Delivery Science, Nemours Children's Health System, 807 Children's Way, Jacksonville, US.

Unstructured: Type 1 diabetes (T1D) management can be challenging for children and their families. To address psychosocial concerns for parents of youth with T1D, we developed two parent-focused interventions to reduce their diabetes distress and fear of hypoglycemia. Our team conducted several of these interventions during the early spread of COVID-19 and recognized a need to make timely adjustments to our interventions. Read More

View Article and Full-Text PDF

MAPLE: A Microbiome Analysis Pipeline Enabling Optimal Peptide Search and Comparative Taxonomic and Functional Analysis.

J Proteome Res 2021 Apr 13. Epub 2021 Apr 13.

Department of Pharmaceutical Sciences, University of Maryland, School of Pharmacy, Baltimore, Maryland 21201, United States.

Metaproteomics by mass spectrometry (MS) is a powerful approach to profile a large number of proteins expressed by all organisms in a highly complex biological or ecological sample, which is able to provide a direct and quantitative assessment of the functional makeup of a microbiota. The human gastrointestinal microbiota has been found playing important roles in human physiology and health, and metaproteomics has been shown to shed light on multiple novel associations between microbiota and diseases. MS-powered proteomics generally relies on genome data to define search space. Read More

View Article and Full-Text PDF

Human telomerase RNA component (hTERC) gene expression and chromosome 7 ploidy correlate positively with histological grade of cervical intraepithelial neoplasia.

Cytopathology 2021 Apr 13. Epub 2021 Apr 13.

Department of Pathology, University College of Medical Sciences & Guru Teg Bahadur Hospital, University of Delhi, Delhi, India.

Objective: Cervical cancer screening by primary human papilloma virus detection and cytology is fraught with low specificity and variable sensitivity, respectively. Cytology-histology correlation remains modest. Biomarkers associated with early genetic events in cervical squamous carcinogenesis and detectable in cytology material are likely to be relevant. Read More

View Article and Full-Text PDF

Making Biological Sense of Genetic Studies of Age-Related Macular Degeneration.

Adv Exp Med Biol 2021 ;1256:201-219

Neurobiology-Neurodegeneration & Repair Laboratory, National Eye Institute, National Institutes of Health, Bethesda, MD, USA.

Age-related macular degeneration (AMD) is a major cause of blindness in older individuals worldwide. The disease is characterized by deposition of drusen between the retinal pigment epithelium (RPE) and Bruch's membrane, RPE atrophy and death of photoreceptors. AMD is a complex disease with multiple genetic and non-genetic risk factors. Read More

View Article and Full-Text PDF
January 2021

SKI activates the Hippo pathway via LIMD1 to inhibit cardiac fibroblast activation.

Basic Res Cardiol 2021 Apr 13;116(1):25. Epub 2021 Apr 13.

Institute of Cardiovascular Sciences, St. Boniface Hospital Albrechtsen Research Centre, 351 Taché Avenue, Winnipeg, MB, Canada.

We have previously shown that overexpression of SKI, an endogenous TGF-β repressor, deactivates the pro-fibrotic myofibroblast phenotype in the heart. We now show that SKI also functions independently of SMAD/TGF-β signaling, by activating the Hippo tumor-suppressor pathway and inhibiting the Transcriptional co-Activator with PDZ-binding motif (TAZ or WWTR1). The mechanism(s) by which SKI targets TAZ to inhibit cardiac fibroblast activation and fibrogenesis remain undefined. Read More

View Article and Full-Text PDF

Identification of differentially expressed miRNAs in human cells infected with different Zika virus strains.

Arch Virol 2021 Apr 13. Epub 2021 Apr 13.

Department of Biology, IBILCE, Institute of Biosciences, Humanities and Exact Sciences, UNESP, São Paulo State University, Rua Cristóvão Colombo, 2265, Bairro Jardim Nazareth, São José do Rio Preto, SP, 15054-010, Brazil.

Infection with distinct Zika virus (ZIKV) strains in in vitro and in vivo models has demonstrated that the host's response to infection is strain-dependent. There has been no analysis of the impact of infection with different ZIKV strains on miRNA expression in human cells. We investigated miRNA expression in PNT1A cells upon infection with an African ZIKV strain (MR766) and a Brazilian ZIKV strain (ZIKV) using PCR array. Read More

View Article and Full-Text PDF

Identification and analysis of key genes associated with acute myocardial infarction by integrated bioinformatics methods.

Medicine (Baltimore) 2021 Apr;100(15):e25553

Department of Clinical Chinese Pharmacy, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.

Background: Acute myocardial infarction (AMI) is a common disease leading threat to human health around the world. Here we aimed to explore new biomarkers and potential therapeutic targets in AMI through adopting integrated bioinformatics tools.

Methods: The gene expression Omnibus (GEO) database was used to obtain genes data of AMI and no-AMI whole blood. Read More

View Article and Full-Text PDF

Exploring risk factors at the molecular level.

Elife 2021 Apr 13;10. Epub 2021 Apr 13.

Faculty of Health, York University, Toronto, Canada.

Risk factors for cardiovascular diseases trigger molecular changes that harm the endothelial cells in the heart, but exercise can suppress these effects. Read More

View Article and Full-Text PDF

Molecular Insights into Myocilin and Its Glaucoma-Causing Misfolded Olfactomedin Domain Variants.

Acc Chem Res 2021 Apr 13. Epub 2021 Apr 13.

School of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Dr. NW, Atlanta, Georgia 30332-0400, United States.

ConspectusNumerous human disorders arise due to the inability of a particular protein to adopt its correct three-dimensional structure in the context of the cell, leading to aggregation. A new addition to the list of such protein conformational disorders is the inherited subtype of glaucoma. Different and rare coding mutations in myocilin, found in families throughout the world, are causal for early onset ocular hypertension, a key glaucoma risk factor. Read More

View Article and Full-Text PDF

High glucose promotes the aging of human dental pulp cells through Wnt/beta-catenin signaling.

Dent Med Probl 2021 Jan-Mar;58(1):39-46

Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Iran.

Background: Diabetes is one of the most common metabolic diseases that disrupt the functioning of different body organs, including oral tissue. Some diabetic complications remain even after the control of the hyperglycemic condition. The adverse effect of hyperglycemia on the pulp structure and function has been shown previously. Read More

View Article and Full-Text PDF

Hspa8 and ICAM-1 as damage-induced mediators of γδ T cell activation.

J Leukoc Biol 2021 Apr 13. Epub 2021 Apr 13.

Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, California, USA.

Tissue-resident γδ T cells form the first line of defense at barrier surfaces where they survey host tissue for signs of stress or damage. Following recognition of injury, γδ T cells play a crucial role in the wound-healing response through the production of growth factors and cytokines that promote proliferation in surrounding epithelial cells. To initiate this response, γδ T cells require interactions with a variety of epithelial-expressed costimulatory molecules in addition to primary signaling through their TCR. Read More

View Article and Full-Text PDF

Effects of metformin on experimentally induced acne on rabbit ear.

Exp Dermatol 2021 Apr 13. Epub 2021 Apr 13.

Department of Dermatology, Venereology and Leprology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

Evidence for the effectiveness of metformin in the treatment of acne is limited. To assess its efficacy, comedones were experimentally induced in young New Zealand rabbit ear using Isopropyl Myristate (IM) followed by metformin treatment (30 mg/kg bodyweight) for 60 days with continued IM application. In another group, to check whether metformin pre-treatment affects subsequent comedone development by IM, metformin was given for 14 days and then withdrawn (14 days) followed by comedone development with IM and metformin treatment. Read More

View Article and Full-Text PDF

GPER mediates estrogen cardioprotection against epinephrine-induced stress.

J Endocrinol 2021 Apr 1. Epub 2021 Apr 1.

H Sun, physiology department, Xuzhou Medical University, Xuzhou, China.

Currently, there are no conventional treatments for stress-induced cardiomyopathy (SCM, also known as Takotsubo syndrome), and the existing therapies are not effective. The recently discovered G protein- coupled estrogen receptor (GPER) executes the rapid effects of estrogen (E2). In this study, we investigated the effects and mechanism of GPER on epinephrine (Epi)-induced cardiac stress. Read More

View Article and Full-Text PDF

SARS‑CoV-2 RBD219-N1C1: A yeast-expressed SARS-CoV-2 recombinant receptor-binding domain candidate vaccine stimulates virus neutralizing antibodies and T-cell immunity in mice.

Hum Vaccin Immunother 2021 Apr 13:1-11. Epub 2021 Apr 13.

Texas Children's Hospital Center for Vaccine Development, Houston, TX, USA.

There is an urgent need for an accessible and low-cost COVID-19 vaccine suitable for low- and middle-income countries. Here, we report on the development of a SARS-CoV-2 receptor-binding domain (RBD) protein, expressed at high levels in yeast (), as a suitable vaccine candidate against COVID-19. After introducing two modifications into the wild-type RBD gene to reduce yeast-derived hyperglycosylation and improve stability during protein expression, we show that the recombinant protein, RBD219-N1C1, is equivalent to the wild-type RBD recombinant protein (RBD219-WT) in an ACE-2 binding assay. Read More

View Article and Full-Text PDF

microRNA-204 shuttled by mesenchymal stem cell-derived exosomes inhibits the migration and invasion of non-small-cell lung cancer cells via the KLF7/AKT/HIF-1α axis.

Neoplasma 2021 Apr 13. Epub 2021 Apr 13.

Department of Respiratory Medicine, The First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, China.

Non-small-cell lung cancer (NSCLC) remains the leading cause of cancer-related death worldwide. Accumulating researches have highlighted the ability of exosome-encapsulated microRNAs (miRNAs or miRs) as potential circulating biomarkers for lung cancer. The current study aimed to evaluate the significance of mesenchymal stem cells (MSCs)-derived exosomal miR-204 in the invasion, migration, and epithelial-mesenchymal transition (EMT) of NSCLC cells. Read More

View Article and Full-Text PDF