10,213 results match your criteria expressed chondrocytes

Caviunin glycoside (CAFG) from Dalbergia sissoo attenuates osteoarthritis by modulating chondrogenic and matrix regulating proteins.

J Ethnopharmacol 2021 Jun 8:114315. Epub 2021 Jun 8.

Endocrinology Division, CSIR-Central Drug Research Institute, Lucknow, 226031, India. Electronic address:

Ethnopharmacological Relevance: Dalbergia sissoo DC. (Indian rosewood or Sheesham) is a traditional medicinal plant, reported since time immemorial for its analgesic, anti-nociceptive, anti-inflammatory, and immuno-modulatory properties. D. Read More

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A chondrosphere-based scaffold free approach to manufacture an in-vitro articular cartilage model.

Tissue Eng Part A 2021 Jun 11. Epub 2021 Jun 11.

Newcastle University, 5994, School of Engineering, Claremont Road, Newcastle upon Tyne, Tyne and Wear, United Kingdom of Great Britain and Northern Ireland, NE1 7RU;

In-vitro engineering of human articular cartilage (AC) is a regenerative medicine challenge. The main objective of this study was the development of a repeatable scaffold-free in-vitro model of chondrocyte spheroid-based treatments of cartilage defects, to allow for systematic study and further optimisation of this type of treatment. Human articular chondrocytes (HC) and immortalised mesenchymal cells differentiated in chondrocytes (Y201-Cs) were cultured in round-bottom 96-well plates to produce multicellular spheroids and their growth kinetics and viability was evaluated over 7 days of culture. Read More

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Rat perichondrium transplanted to articular cartilage defects forms articular-like, hyaline cartilage.

Bone 2021 Jun 7:116035. Epub 2021 Jun 7.

Division of Pediatric Endocrinology and Center for Molecular Medicine, L8:01, Department of Women's and Children's Health, Karolinska Institutet and University Hospital, Stockholm, Sweden; School of Medical Sciences, Örebro University and University Hospital, Örebro, Sweden. Electronic address:

Objective: Perichondrium autotransplants have been used to reconstruct articular surfaces destroyed by infection or trauma. However, the role of the transplanted perichondrium in the healing of resurfaced joints have not been investigated.

Design: Perichondrial and periosteal tissues were harvested from rats hemizygous for a ubiquitously expressed enhanced green fluorescent protein (EGFP) transgene and transplanted into full-thickness articular cartilage defects at the trochlear groove of distal femur in wild-type littermates. Read More

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Computational and experimental studies of a cell-imprinted-based integrated microfluidic device for biomedical applications.

Sci Rep 2021 Jun 9;11(1):12130. Epub 2021 Jun 9.

National Cell Bank Department, Pasteur Institute of Iran, P.O. Box 13169-43551, Tehran, Iran.

It has been proved that cell-imprinted substrates molded from template cells can be used for the re-culture of that cell while preserving its normal behavior or to differentiate the cultured stem cells into the template cell. In this study, a microfluidic device was presented to modify the previous irregular cell-imprinted substrate and increase imprinting efficiency by regular and objective cell culture. First, a cell-imprinted substrate from template cells was prepared using a microfluidic chip in a regular pattern. Read More

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Identification of Differentially Expressed circRNAs, miRNAs, and Genes in Patients Associated with Cartilaginous Endplate Degeneration.

Biomed Res Int 2021 18;2021:2545459. Epub 2021 May 18.

Department of Minimally Invasive Spine Surgery, Tianjin Hospital, No. 406, Jiefangnan Road, Hexi District, Tianjin 300211, China.

Background: Intervertebral disc degeneration (IDD) disease is a global challenge because of its predominant pathogenic factor in triggering low back pain, whereas cartilaginous endplate degeneration (CEPD) is the main cause of IDD. Accumulating evidence have indicated that the differentially expressed microRNAs (DEMs) and differentially expressed genes (DEGs) have been determined to be involved in multiple biological processes to mediate CEPD progression. However, the differentially expressed circular RNAs (DECs) and their potential biofunctions in CEPD have not been identified. Read More

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Downregulation of miR-892b inhibits the progression of osteoarthritis via targeting Cyclin D1 and Cyclin D2.

Exp Cell Res 2021 Jun 5:112683. Epub 2021 Jun 5.

Department of Orthopedic Surgery, Henan Provincial People's Hospital; Zhengzhou University People's Hospital, Zhengzhou, Henan 450003, China. Electronic address:

Backgrounds: Osteoarthritis (OA) is an orthopedic inflammatory disease which can cause functional disability and chronic pain. MiRNAs are known to play important roles in OA. To identify the targets for the treatment of OA, bioinformatics analysis was performed to explore differentially expressed miRNAs between OA and normal samples. Read More

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H3K36 methyltransferase NSD1 regulates chondrocyte differentiation for skeletal development and fracture repair.

Bone Res 2021 Jun 7;9(1):30. Epub 2021 Jun 7.

Shanghai Institute of Microsurgery on Extremities, and Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Chondrocyte differentiation is a critical process for endochondral ossification, which is responsible for long bone development and fracture repair. Considerable progress has been made in understanding the transcriptional control of chondrocyte differentiation; however, epigenetic regulation of chondrocyte differentiation remains to be further studied. NSD1 is a H3K36 (histone H3 at lysine 36) methyltransferase. Read More

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DDIT3/CHOP promotes autophagy in chondrocytes via SIRT1-AKT pathway.

Biochim Biophys Acta Mol Cell Res 2021 Jun 2;1868(9):119074. Epub 2021 Jun 2.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, Hubei, China. Electronic address:

Endoplasmic reticulum (ER) stress can initiate autophagy via unfolded protein response (UPR). As a key downstream gene of UPR, DDIT3/CHOP is expressed in chondrocytes. However, the regulation mechanism of DDIT3/CHOP on autophagy in chondrocytes remains unclear. Read More

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Circ_HECW2 regulates LPS-induced apoptosis of chondrocytes via miR-93 methylation.

Immun Inflamm Dis 2021 Jun 2. Epub 2021 Jun 2.

Department of Sports Medicine, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.

Introduction: Circ_HECW2 plays a key role in lipopolysaccharide (LPS)-induced signal transduction, which is critical in osteoarthritis (OA). Thus, we analyzed the role of Circ_HECW2 in osteoarthritis.

Methods: The expression of Circ_HECW2 and miR-93 was examined using reverse-transcription polymerase chain reaction. Read More

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MiR-26a regulates the expression of serum IGF-1 in patients with osteoporosis and its effect on proliferation and apoptosis of mouse chondrocytes.

J Musculoskelet Neuronal Interact 2021 Jun;21(2):298-307

Orthopedic Department, Rizhao People's Hospital, P.R. China.

Objectives: To examine the effects of the regulation on IGF-1 by miR-26a on the serum of patients with osteoporosis (OP) and apoptosis and proliferation of chondrocytes of mice with OP.

Methods: Totally 47 patients with OP treated in our hospital between July 2018 and November 2019 were selected as the research group, and 42 healthy individuals in physical examination over this period were selected as the control group. Serum was sampled from each participant in both groups, and miR-26a in the sampled serum was quantified and compared. Read More

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Interleukin-17A Causes Osteoarthritis-Like Transcriptional Changes in Human Osteoarthritis-Derived Chondrocytes and Synovial Fibroblasts .

Front Immunol 2021 12;12:676173. Epub 2021 May 12.

The Botnar Research Centre, Nuffield Department of Orthopaedics Rheumatology and Musculoskeletal Science, University of Oxford, Oxford, United Kingdom.

Increased interleukin (IL)-17A has been identified in joints affected by osteoarthritis (OA), but it is unclear how IL-17A, and its family members IL-17AF and IL-17F, can contribute to human OA pathophysiology. Therefore, we aimed to evaluate the gene expression and signalling pathway activation effects of the different IL-17 family members in chondrocytes and synovial fibroblasts derived from cartilage and synovium of patients with end-stage knee OA. Immunohistochemistry staining confirmed that IL-17 receptor A (IL-17RA) and IL-17RC are expressed in end-stage OA-derived cartilage and synovium. Read More

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miR-520c-3p regulates IL-1β-stimulated human chondrocyte apoptosis and cartilage degradation by targeting GAS2.

J Orthop Surg Res 2021 May 29;16(1):347. Epub 2021 May 29.

Department of Orthopaedics, Renmin Hospital of Wuhan University, No. 238, Jiefang Road, Wuchang District, Wuhan City, 430060, Hubei Province, China.

Background: MicroRNAs (miRNAs) have been shown to be associated with osteoarthritis (OA) progression. This study aimed to explore the role of miR-520c-3p in OA progression.

Methods: Expression levels of miR-520c-3p and Growth arrest-specific 2 (GAS2) were detected using quantitative real-time PCR. Read More

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Azilsartan prevented AGE-induced inflammatory response and degradation of aggrecan in human chondrocytes through inhibition of Sox4.

J Biochem Mol Toxicol 2021 May 29:e22827. Epub 2021 May 29.

Bone Injury Diagnosis and Treatment Center, Hubei Provincial Hospital Of TCM, Wuhan, Hubei, China.

Advanced glycation end products (AGEs)-induced inflammation and degradation of aggrecan in human chondrocytes play an important role in the progression and development of osteoarthritis (OA). Azilsartan, an angiotensin II receptor antagonist, has been licensed for the treatment of high blood pressure. However, the effects of Azilsartan in OA and AGEs-induced damages in chondrocytes have not been previously reported. Read More

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Comparison of the major cell populations among osteoarthritis, Kashin-Beck disease and healthy chondrocytes by single-cell RNA-seq analysis.

Cell Death Dis 2021 May 27;12(6):551. Epub 2021 May 27.

School of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, 710061, Xi'an, Shaanxi, P. R. China.

Chondrocytes are the key target cells of the cartilage degeneration that occurs in Kashin-Beck disease (KBD) and osteoarthritis (OA). However, the heterogeneity of articular cartilage cell types present in KBD and OA patients and healthy controls is still unknown, which has prevented the study of the pathophysiology of the mechanisms underlying the roles of different populations of chondrocytes in the processes leading to KBD and OA. Here, we aimed to identify the transcriptional programmes and all major cell populations in patients with KBD, patients with OA and healthy controls to identify the markers that discriminate among chondrocytes in these three groups. Read More

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Influence of swimming exercise on the expression of apoptotic gene caspase-3 in chondrocytes in osteoarthritis.

Am J Transl Res 2021 15;13(4):2511-2517. Epub 2021 Apr 15.

Department of Traumatic Orthopedics, The First Hospital of Jilin University Changchun, Jilin, China.

Objective: To study swimming exercise's effect on caspase-3 expression in chondrocytes in osteoarthritis (OA).

Methods: 36 SD rats were randomly separated into normal group (n = 12), OA model group (n = 12) and swimming exercise group (n = 12). After modeling, rats received no intervention in model group and swimming exercise once a day (15 min/time) for 4 consecutive weeks in swimming exercise group. Read More

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Oxygen-generating microparticles in chondrocytes-laden hydrogels by facile and versatile click chemistry strategy.

Colloids Surf B Biointerfaces 2021 May 13;205:111850. Epub 2021 May 13.

LIMAV-Interdisciplinary Laboratory for Advanced Materials, BioMatLab, UFPI - Federal University of Piaui, Teresina, PI, 64049-550, Brazil; Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA, 02139, United States. Electronic address:

Currently, oxygen supply for in vitro cell culture is one of the major challenges in tissue engineering, especially in three-dimensional (3D) structures, such as polymeric hydrogels, because oxygen is an essential element for cells survival. In this context, oxygen levels must be maintained in articular cartilage to promote the differentiation, viability, and proliferation of chondrocytes due to the low level of oxygen presence in this region. Although some technologies employ oxygen-generating materials to add sufficient oxygen levels, the limitations and challenges of current technologies include the lack of controlled, sustained, and prolonged release of the oxygen. Read More

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Roles of long non‑coding RNA in osteoarthritis (Review).

Int J Mol Med 2021 Jul 20;48(1). Epub 2021 May 20.

Department of Joint Surgery, Weifang People's Hospital, Weifang, Shandong 261000, P.R. China.

Osteoarthritis (OA) is a chronic bone and joint disease characterized by articular cartilage degeneration and joint inflammation and is the most common form of arthritis. The clinical manifestations of OA are chronic pain and joint activity disorder, which severely affect the patient quality of life. Long non‑coding RNA (lncRNA) is a class of RNA molecules >200 nucleotides long that are expressed in animals, plants, yeast, prokaryotes and viruses. Read More

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Hox11 expression characterizes developing zeugopod synovial joints and is coupled to postnatal articular cartilage morphogenesis into functional zones in mice.

Dev Biol 2021 May 16;477:49-63. Epub 2021 May 16.

Translational Research Program in Pediatric Orthopaedics, Division of Orthopaedic Surgery, The Children's Hospital of Philadelphia, Philadelphia, PA, USA.

Previous studies on mouse embryo limbs have established that interzone mesenchymal progenitor cells emerging at each prescribed joint site give rise to joint tissues over fetal time. These incipient tissues undergo structural maturation and morphogenesis postnatally, but underlying mechanisms of regulation remain unknown. Hox11 genes dictate overall zeugopod musculoskeletal patterning and skeletal element identities during development. Read More

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The role of microRNAs in the osteogenic and chondrogenic differentiation of mesenchymal stem cells and bone pathologies.

Theranostics 2021 30;11(13):6573-6591. Epub 2021 Apr 30.

Department of Medical Sciences, Section of Experimental Medicine, School of Medicine, University of Ferrara. Ferrara, Italy.

Mesenchymal stem cells (MSCs) have been identified in many adult tissues. MSCs can regenerate through cell division or differentiate into adipocytes, osteoblasts and chondrocytes. As a result, MSCs have become an important source of cells in tissue engineering and regenerative medicine for bone tissue and cartilage. Read More

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Sodium Monoiodoacetate Dose-Dependent Changes in Matrix Metalloproteinases and Inflammatory Components as Prognostic Factors for the Progression of Osteoarthritis.

Front Pharmacol 2021 28;12:643605. Epub 2021 Apr 28.

Department of Neurochemistry, Maj Institute of Pharmacology, Polish Academy of Sciences, Cracow, Poland.

Osteoarthritis (OA) is a degenerative joint disease that primarily affects people over 65 years old. During OA progression irreversible cartilage, synovial membrane and subchondral bone degradation is observed, which results in the development of difficult-to-treat chronic pain. One of the most important factors in OA progression is joint inflammation. Read More

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Intra-articular silencing of periostin via nanoparticle-based siRNA ameliorates post-traumatic osteoarthritis in mice.

Arthritis Rheumatol 2021 May 13. Epub 2021 May 13.

Department of Orthopaedic Surgery, Musculoskeletal Research Center, Washington University, School of Medicine, St. Louis, MO, United States.

Objective: Recent evidence delineates an emerging role of Periostin (Postn) in osteoarthritis (OA) as its expression subsequent to knee injury is detrimental to the articular cartilage. We hypothesize that intra-articular knockdown of Postn in a murine model of post-traumatic OA would ameliorate OA.

Methods: Post-traumatic OA was induced in 10-week-old male C57BL/6J mice (n=24) by destabilization of the medial meniscus (DMM) and analyzed 8-week post-surgery. Read More

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Long noncoding RNA TUG1 regulates degradation of chondrocyte extracellular matrix via miR-320c/MMP-13 axis in osteoarthritis.

Hu Han Lijuan Liu

Open Life Sci 2021 21;16(1):384-394. Epub 2021 Apr 21.

Department of Rehabilitation, The First People's Hospital of Jingmen, No. 67 Xiangshan Dadao, Dongbao District, Jingmen 448000, Hubei, China.

Osteoarthritis (OA) is a common chronic joint disease. This study aimed to explore the function of long noncoding RNA taurine-upregulated gene 1 (TUG1) in the progression and initiation of OA. Levels of TUG1, microRNA-320c (miR-320c) and fucosyltransferase 4 (FUT4) were examined via quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Read More

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The culture microenvironment of juvenile idiopathic arthritis synovial fibroblasts is favorable for endochondral bone formation through BMP4 and repressed by chondrocytes.

Pediatr Rheumatol Online J 2021 May 12;19(1):72. Epub 2021 May 12.

Division of Rheumatology, Nemours/Alfred I. duPont Hospital for Children, 1701 Rockland Rd, Wilmington, DE, 19803, USA.

Background: We examined influences of conditioned media from chondrocytes (Ch) on juvenile idiopathic arthritis synovial fibroblasts (JFLS) and potential for JFLS to undergo endochondral bone formation (EBF).

Methods: Primary cells from three control fibroblast-like synoviocytes (CFLS) and three JFLS were cultured in Ch-conditioned media and compared with untreated fibroblast-like synoviocytes (FLS). RNA was analyzed by ClariomS microarray. Read More

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DNA-Grafted Hyaluronic Acid System with Enhanced Injectability and Biostability for Photo-Controlled Osteoarthritis Gene Therapy.

Adv Sci (Weinh) 2021 05 1;8(9):2004793. Epub 2021 Mar 1.

Department of Orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases Shanghai Institute of Traumatology and Orthopaedics Ruijin Hospital Shanghai Jiao Tong University School of Medicine 197 Ruijin 2nd Road Shanghai 200025 P. R. China.

Gene therapy is identified as a powerful strategy to overcome the limitations of traditional therapeutics to achieve satisfactory effects. However, various challenges related to the dosage form, delivery method, and, especially, application value, hampered the clinical transition of gene therapy. Here, aiming to regulate the cartilage inflammation and degeneration related abnormal IL-1 mRNA expression in osteoarthritis (OA), the interference oligonucleotides is integrated with the Au nanorods to fabricate the spherical nucleic acids (SNAs), to promote the stability and cell internalization efficiency. Read More

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Punicalin alleviates TNF-α- and IL-1β-induced chondrocyte dysfunction and cartilage metabolism via mediating FOXO3 signaling axis.

J Food Biochem 2021 May 11:e13755. Epub 2021 May 11.

Department of Rheumatology, Zibo Central Hospital, Zibo, P.R. China.

Forkhead box O3 (FOXO3) transcription factor is involved in chondral homeostasis of normal, aging and osteoarthritis (OA) cartilage. At present, we aimed to investigate whether FOXO3 is a target of punicalin to prevent IL-1β- and TNF-α-induced chondrocyte dysfunction in vitro and in vivo models. Cell and mouse models of chondrocyte dysfunction were established to determine the pharmacological value of hydrolyzable tannin, punicalin, which was extracted from the pomegranate. Read More

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Adenosine A2A receptor null chondrocyte transcriptome resembles that of human osteoarthritic chondrocytes.

Purinergic Signal 2021 May 11. Epub 2021 May 11.

Department of Medicine, Division of Rheumatology, NYUGSOM, New York, NY, USA.

Adenosine signaling plays a critical role in the maintenance of articular cartilage and may serve as a novel therapeutic for osteoarthritis (OA), a highly prevalent and morbid disease without effective therapeutics in the current market. Mice lacking adenosine A2A receptors (A2AR) develop spontaneous OA by 16 weeks of age, a finding relevant to human OA since loss of adenosine signaling due to diminished adenosine production (NT5E deficiency) also leads to development of OA in mice and humans. To better understand the mechanism by which A2AR and adenosine generation protect from OA development, we examined differential gene expression in neonatal chondrocytes from WT and A2AR null mice. Read More

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CircATRNL1 protects against osteoarthritis by targeting miR-153-3p and KLF5.

Int Immunopharmacol 2021 May 7;96:107704. Epub 2021 May 7.

Department of Orthopaedics, the 1st Affiliated Hospital of Harbin Medical University, Harbin 150001, China. Electronic address:

Background: Osteoarthritis (OA) is characterized by chondrocyte injury. Circular RNAs (circRNAs) are involved in the pathogenesis of various diseases, including OA. The purpose of this study was to determine the potential role of circATRNL1 in OA pathology in vitro. Read More

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Valproic Acid Protects Chondrocytes from LPS-Stimulated Damage via Regulating miR-302d-3p/ITGB4 Axis and Mediating the PI3K-AKT Signaling Pathway.

Front Mol Biosci 2021 22;8:633315. Epub 2021 Apr 22.

Department of Emergency, People's Hospital of Rizhao, Rizhao, China.

Osteoarthritis (OA) is one of the most common degenerative joint diseases characterized by increased apoptosis and autophagy deficiency. The investigation was performed to examine the effect of valproic acid (VPA) and molecular mechanism related to miR-302d-3p/ITGB4 axis in OA. The OA clinical samples were obtained from the GEO database to analyze differentially expressed genes. Read More

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Genetic association scan of 32 osteoarthritis susceptibility genes identified TP63 associated with an endemic osteoarthritis, Kashin-Beck disease.

Bone 2021 May 6;150:115997. Epub 2021 May 6.

Key Laboratory of Trace Elements and Endemic Diseases, Collaborative Innovation Center of Endemic Disease and Health Promotion for Silk Road Region, School of Public Health, Health Science Center, Xi'an Jiaotong University, 710061, China. Electronic address:

Background: Kashin-Beck disease (KBD) is an endemic chronic osteochondropathy. The clinical manifestations and radiographic features of adult KBD were similar to those of osteoarthritis (OA).

Methods: We first performed a genetic association scan of 32 OA susceptibility genes with KBD in 898 Han Chinese subjects. Read More

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MiR-485-3p promotes proliferation of osteoarthritis chondrocytes and inhibits apoptosis via Notch2 and the NF-κB pathway.

Immunopharmacol Immunotoxicol 2021 Jun 7;43(3):370-379. Epub 2021 May 7.

Department of Foot and Ankle Surgery, Honghui Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, China.

Context: Osteoarthritis (OA) is one of the leading causes of disability worldwide. microRNAs (miRs) has been shown to be involved in multiple pathological processes during OA. But the possible mechanism of miR-485-3p in OA remains unclear. Read More

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