15 results match your criteria exposed renca

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Tissue-specific tumor microenvironments influence responses to immunotherapies.

Clin Transl Immunology 2019 21;8(11):e1094. Epub 2019 Nov 21.

Cancer Immunology Program Peter MacCallum Cancer Centre Melbourne VIC Australia.

Objectives: Investigation of variable response rates to cancer immunotherapies has exposed the immunosuppressive tumor microenvironment (TME) as a limiting factor of therapeutic efficacy. A determinant of TME composition is the tumor location, and clinical data have revealed associations between certain metastatic sites and reduced responses. Preclinical models to study tissue-specific TMEs have eliminated genetic heterogeneity, but have investigated models with limited clinical relevance. Read More

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November 2019

Mercury and methylmercury levels in soils associated with coal-fired power plants in central-northern Chile.

Chemosphere 2019 Dec 7;237:124535. Epub 2019 Aug 7.

Laboratorio de Química Analítica y Ambiental, Instituto de Química, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.

Mercury pollution is a worldwide problem, and is associated with a number of natural and anthropogenic processes. The present work, conducted in Chile, a country that has traditionally depended heavily on fossil fuels for power generation, examines total mercury (THg) and monomethylmercury (MMHg) concentrations in soils across different sites exposed to coal fired power plant emissions. Samples from four selected (Renca, Laguna Verde, Las Ventanas, Huasco) and 1 control (Quintay) sites were analyzed using cold vapour and fluorescence spectroscopy (CV-AFS) for THg determination and chromatographic separation with atomic fluorescence detection (DI-GC-AFS) was followed for speciation analysis. Read More

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December 2019

MEF2 plays a significant role in the tumor inhibitory mechanism of encapsulated RENCA cells via EGF receptor signaling in target tumor cells.

BMC Cancer 2018 Dec 4;18(1):1217. Epub 2018 Dec 4.

The Rogosin Institute-Xenia Division, 740 Birch Road, Xenia, OH, 45385, USA.

Background: Agarose encapsulated murine renal adenocarcinoma cells (RENCA macrobeads) are currently being investigated in clinical trials as a treatment for therapy-resistant metastatic colorectal cancer. We have previously demonstrated the capacity of RENCA macrobeads to produce diffusible substances that markedly inhibit the proliferation of epithelial-derived tumor cells outside the macrobead environment. This study examined the molecular mechanisms underlying the observed inhibition in targeted tumor cells exposed to RENCA macrobeads. Read More

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December 2018

Impact of Chemical-Induced Mutational Load Increase on Immune Checkpoint Therapy in Poorly Responsive Murine Tumors.

Mol Cancer Ther 2018 04 26;17(4):869-882. Epub 2018 Feb 26.

Biological Sciences Platform, Sunnybrook Research Institute, Toronto, Canada.

A recurring historic finding in cancer drug development is encouraging antitumor effects observed in tumor-bearing mice that fail to translate into the clinic. An intriguing exception to this pattern is immune checkpoint therapy, as the sustained tumor regressions observed in subsets of cancer patients are rare in mice. Reasoning that this may be due in part to relatively low mutational loads of mouse tumors, we mutagenized transplantable mouse tumor cell lines EMT-6/P, B16F1, RENCA, CT26, and MC38 with methylnitro-nitrosoguanidine (MNNG) or ethylmethane sulfonate (EMS) and tested their responsiveness to PD-L1 blockade. Read More

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Intermittent hypoxia increases kidney tumor vascularization in a murine model of sleep apnea.

PLoS One 2017 8;12(6):e0179444. Epub 2017 Jun 8.

Unitat de Biofísica i Bioenginyeria, Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona, Barcelona, Spain.

We investigate the effects of intermittent hypoxia (IH), a characteristic feature of obstructive sleep apnea (OSA), on renal cancer progression in an animal and cell model. An in vivo mouse model (Balb/c, n = 50) of kidney cancer was used to assess the effect of IH on tumor growth, metastatic capacity, angiogenesis and tumor immune response. An in vitro model tested the effect of IH on RENCA cells, macrophages and endothelial cells. Read More

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September 2017

A CCR4 antagonist reverses the tumor-promoting microenvironment of renal cancer.

J Clin Invest 2017 Mar 30;127(3):801-813. Epub 2017 Jan 30.

Elevated expression of the chemokine receptor CCR4 in tumors is associated with poor prognosis in several cancers. Here, we have determined that CCR4 was highly expressed in human renal cell carcinoma (RCC) biopsies and observed abnormal levels of CCR4 ligands in RCC patient plasma. An antagonistic anti-CCR4 antibody had antitumor activity in the RENCA mouse model of RCC. Read More

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Cinnamic aldehyde suppresses hypoxia-induced angiogenesis via inhibition of hypoxia-inducible factor-1α expression during tumor progression.

Biochem Pharmacol 2015 Nov 20;98(1):41-50. Epub 2015 Aug 20.

Department of Biomedical Science, School of Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea; Department of Anatomy and Neurobiology, School of Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea. Electronic address:

During tumor progression, hypoxia-inducible factor 1 (HIF-1) plays a critical role in tumor angiogenesis and tumor growth by regulating the transcription of several genes in response to a hypoxic environment and changes in growth factors. This study was designed to investigate the effects of cinnamic aldehyde (CA) on tumor growth and angiogenesis and the mechanisms underlying CA's anti-angiogenic activities. We found that CA administration inhibits tumor growth and blocks tumor angiogenesis in BALB/c mice. Read More

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November 2015

Characterization of mechanism involved in acquired resistance to sorafenib in a mouse renal cell cancer RenCa model.

Clin Transl Oncol 2014 Sep 20;16(9):801-6. Epub 2013 Dec 20.

Division of Urology, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, 650-0017, Japan.

Purpose: The objective of this study was to investigate the mechanism mediating the acquisition of a resistant phenotype to sorafenib in renal cell carcinoma (RCC).

Methods: A parental mouse RCC cell line, RenCa (RenCa/P), was continuously exposed to increasing doses of sorafenib, and a cell line resistant to sorafenib (RenCa/R), showing an approximately sixfold higher IC(50) than that of RenCa/P, was established. Changes in the expression of several molecules in these cell lines following sorafenib treatment were evaluated by western blotting, and the effects of sorafenib treatment on the in vivo growth patterns were compared. Read More

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September 2014

Interferon-gamma-induced nitric oxide inhibits the proliferation of murine renal cell carcinoma cells.

Int J Biol Sci 2012 6;8(8):1109-20. Epub 2012 Sep 6.

Stanley S. Scott Cancer Center, LSUHSC, New Orleans, LA, 70112, USA.

Renal cell carcinoma (RCC) remains one of the most resistant tumors to systemic chemotherapy, radiotherapy, and immunotherapy. Despite great progress in understanding the basic biology of RCC, the rate of responses in animal models and clinical trials using interferons (IFNs) has not improved significantly. It is likely that the lack of responses can be due to the tumor's ability to develop tumor escape strategies. Read More

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February 2013

Hydrophilic agarose macrobead cultures select for outgrowth of carcinoma cell populations that can restrict tumor growth.

Cancer Res 2011 Feb 24;71(3):725-35. Epub 2011 Jan 24.

The Rogosin Institute, New York, New York 10021, USA.

Cancer cells and their associated tumors have long been considered to exhibit unregulated proliferation or growth. However, a substantial body of evidence indicates that tumor growth is subject to both positive and negative regulatory controls. Here, we describe a novel property of tumor growth regulation that is neither species nor tumor-type specific. Read More

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February 2011

Single walled carbon nanohorns as photothermal cancer agents.

Lasers Surg Med 2011 Jan;43(1):43-51

Department of Mechanical Engineering and School of Biomedical Engineering and Sciences, Virginia Tech, Virginia 24061, USA.

Background: Nanoparticles have significant potential as selective photo-absorbing agents for laser based cancer treatment. This study investigates the use of single walled carbon nanohorns (SWNHs) as thermal enhancers when excited by near infrared (NIR) light for tumor cell destruction.

Methods: Absorption spectra of SWNHs in deionized water at concentrations of 0, 0. Read More

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January 2011

Differences between cytotoxicity in photodynamic therapy using a pulsed laser and a continuous wave laser: study of oxygen consumption and photobleaching.

Lasers Med Sci 2004 31;18(4):179-83. Epub 2004 Jan 31.

Department of Medical Engineering, National Defense Medical College, 3-2 Namiki, Tokorozawa, 359-8513, Saitama, Japan.

Oxygen consumption at the targeted site has a significant effect on dosimetry in photodynamic therapy (PDT). However, oxygen consumption in PDT using a pulsed laser as a light source has not been clarified. We therefore investigated the dependence of cytotoxicity on the oxygen consumption and the photosensitizer photobleaching of PDT using a pulsed laser by comparing with that using a continuous wave (CW) laser. Read More

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Selenium supplementation sensitizes renca cells to tert-butylhydroperoxide induced loss of viability.

Indian J Exp Biol 2000 Oct;38(10):1020-5

Department of Physiology, Faculty of Medicine, Adnan Menderes University, Aydin, Turkey.

Mouse renal carcinoma (renca) cells growing exponentially in foetal bovine serum (1%) supplemented with selenium (1 microM, sodium selenite) were exposed to oxidative insult. It was found that glutathione peroxidase activity increased (44%), while the activities of catalase, glutathione disulfide reductase, and level of total glutathione did not change due to selenium supplementation. Selenium supplementation made renca cells susceptible to tert-butylhydroperoxide induced cell death, while it did not affect the viability when the cells were exposed to hydrogen peroxide. Read More

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October 2000

Silencing of the caspase-1 gene occurs in murine and human renal cancer cells and causes solid tumor growth in vivo.

Int J Cancer 2001 Mar;91(5):673-9

Department of Urology, University of Tokyo Hospital, Tokyo, Japan.

Renal cell cancer is a unique solid tumor that occasionally shows spontaneous regression even at an advanced stage, of which the underlying mechanism is not well understood. To investigate a potential role of the pro-apoptotic molecule caspase-1 in the growth regulation of renal cell cancer, we created transfectants expressing exogenous caspase-1 from a murine renal cancer cell line, Renca. Overexpression of caspase-1 did not affect the growth of Renca cells in vitro at the exponential phase but induced apoptotic cell death at 50% to 75% confluence, whereas control cells underwent apoptosis only after reaching 100% confluence. Read More

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Efficacy of multiple administrations of a recombinant adenovirus expressing wild-type p53 in an immune-competent mouse tumor model.

Gene Ther 1998 May;5(5):605-13

Medical Breast Cancer Section, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.

Infection of Renca cells in vitro with a recombinant adenovirus expressing a marker gene beta-galactosidase resulted in high level of the transgene expression. Renca tumors grown in Balb/C mice were also infectable with this recombinant adenovirus. The transgene expression in the tumors lasted for about 7 days, however, administration of another dose of Ad-beta gal, on day 7 produced beta-galactosidase expression. Read More

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