11,607 results match your criteria experimentally identified


Identification of a metallothionein gene and the role of biological thiols in stress induced by short-term Cd exposure in Ostrinia nubilalis.

Comp Biochem Physiol C Toxicol Pharmacol 2021 Jul 31:109148. Epub 2021 Jul 31.

University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology, Trg Dositeja Obradovića 3, 21000 Novi Sad, Serbia.

Cadmium (Cd) is a non-essential metal that is highly toxic to all living forms, characterized by an extremely high affinity for thiol (SH) groups. The aim of this work was to identify and experimentally verify metallothionein gene and to analyze the role of biological thiols in stress induced by short-term Cd exposure in Ostrinia nubilalis, one of the most important corn pests. The coding region of a metallothionein (MT) gene in O. Read More

View Article and Full-Text PDF

A reflux-and-growth mechanism explains oscillatory patterning of lateral root branching sites.

Dev Cell 2021 Jul 27. Epub 2021 Jul 27.

Computational Developmental Biology, Department of Biology, Utrecht University, Utrecht, the Netherlands. Electronic address:

Modular, repetitive structures are a key component of complex multicellular body plans across the tree of life. Typically, these structures are prepatterned by temporal oscillations in gene expression or signaling. Although a clock-and-wavefront mechanism was identified and plant leaf phyllotaxis arises from a Turing-type patterning for vertebrate somitogenesis and arthropod segmentation, the mechanism underlying lateral root patterning has remained elusive. Read More

View Article and Full-Text PDF

Simulation of Cluster Dynamics of Proton-Bound Water Clusters in a High Kinetic Energy Ion-Mobility Spectrometer.

J Am Soc Mass Spectrom 2021 Aug 3. Epub 2021 Aug 3.

Department of Physical and Theoretical Chemistry, University of Wuppertal, Gauss Straße 20, 42119 Wuppertal, Germany.

Ions are separated in ion mobility spectrometry (IMS) by their characteristic motion through a gas-filled drift tube with a static electric field present. Chemical dynamics, such as clustering and declustering of chemically reactive systems, and physical parameters, as, for example, the electric field strength or background gas temperature, impact on the observed ion mobility. In high kinetic energy IMS (HiKE-IMS), the reduced electric field strength is up to 120 Td in both the reaction region and drift region of the instrument. Read More

View Article and Full-Text PDF

Transcriptomic links to muscle mass loss and declines in cumulative muscle protein synthesis during short-term disuse in healthy younger humans.

FASEB J 2021 Sep;35(9):e21830

MRC-Versus Arthritis Centre for Musculoskeletal Ageing Research and National Institute of Health Research, Nottingham Biomedical Research Centre, Royal Derby Hospital Centre, School of Medicine, University of Nottingham, Derby, UK.

Muscle disuse leads to a rapid decline in muscle mass, with reduced muscle protein synthesis (MPS) considered the primary physiological mechanism. Here, we employed a systems biology approach to uncover molecular networks and key molecular candidates that quantitatively link to the degree of muscle atrophy and/or extent of decline in MPS during short-term disuse in humans. After consuming a bolus dose of deuterium oxide (D O; 3 mL. Read More

View Article and Full-Text PDF
September 2021

Distinct mutations in importin-β family nucleocytoplasmic transport receptors transportin-SR and importin-13 affect specific cargo binding.

Sci Rep 2021 Aug 2;11(1):15649. Epub 2021 Aug 2.

Cellular Dynamics Laboratory, RIKEN Cluster for Pioneering Research, Wako, Saitama, Japan.

Importin-(Imp)β family nucleocytoplasmic transport receptors (NTRs) are supposed to bind to their cargoes through interaction between a confined interface on an NTR and a nuclear localization or export signal (NLS/NES) on a cargo. Although consensus NLS/NES sequence motifs have been defined for cargoes of some NTRs, many experimentally identified cargoes of those NTRs lack those motifs, and consensus NLSs/NESs have been reported for only a few NTRs. Crystal structures of NTR-cargo complexes have exemplified 3D structure-dependent binding of cargoes lacking a consensus NLS/NES to different sites on an NTR. Read More

View Article and Full-Text PDF

Identification of cell lines CL-14, CL-40 and CAL-51 as suitable models for SARS-CoV-2 infection studies.

PLoS One 2021 2;16(8):e0255622. Epub 2021 Aug 2.

Department of Human and Animal Cell Lines, Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.

The SARS-CoV-2 pandemic is a major global threat that sparked global research efforts. Pre-clinical and biochemical SARS-CoV-2 studies firstly rely on cell culture experiments where the importance of choosing an appropriate cell culture model is often underestimated. We here present a bottom-up approach to identify suitable permissive cancer cell lines for drug screening and virus research. Read More

View Article and Full-Text PDF

Transcript pattern analysis of Arf-family genes in the phytopathogen Fusarium oxysporum f. sp. lycopersici reveals the role of Arl3 in the virulence.

Antonie Van Leeuwenhoek 2021 Aug 2. Epub 2021 Aug 2.

Laboratorio de Diferenciación Celular, Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Ciudad Universitaria, 58038, Morelia, Michoacán, Mexico.

Fusarium oxysporum f. sp. lycopersici is an important plant pathogen that has been used to understand the virulence mechanisms that soil inhabiting fungi exhibit during the infection process. Read More

View Article and Full-Text PDF

Flow-dependent regulation of endothelial Tie2 by GATA3 in vivo.

Intensive Care Med Exp 2021 Aug 2;9(1):38. Epub 2021 Aug 2.

Department of Nephrology and Hypertension, Hannover Medical School, Hannover, Germany.

Background: Reduced endothelial Tie2 expression occurs in diverse experimental models of critical illness, and experimental Tie2 suppression is sufficient to increase spontaneous vascular permeability. Looking for a common denominator among different critical illnesses that could drive the same Tie2 suppressive (thereby leak inducing) phenotype, we identified "circulatory shock" as a shared feature and postulated a flow-dependency of Tie2 gene expression in a GATA3 dependent manner. Here, we analyzed if this mechanism of flow-regulation of gene expression exists in vivo in the absence of inflammation. Read More

View Article and Full-Text PDF

Multi-omic profiling of plasma reveals molecular alterations in children with COVID-19.

Theranostics 2021 6;11(16):8008-8026. Epub 2021 Jul 6.

Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, 510120, China.

Children usually develop less severe symptoms responding to Coronavirus Disease 2019 (COVID-19) than adults. However, little is known about the molecular alterations and pathogenesis of COVID-19 in children. We conducted plasma proteomic and metabolomic profilings of the blood samples of a cohort containing 18 COVID-19-children with mild symptoms and 12 healthy children, which were enrolled from hospital admissions and outpatients, respectively. Read More

View Article and Full-Text PDF

Systems pharmacology approach uncovers the therapeutic mechanism of medicarpin against scopolamine-induced memory loss.

Phytomedicine 2021 Jul 16;91:153662. Epub 2021 Jul 16.

Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China; Department of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, CA, 94305, United States. Electronic address:

Background: Medicarpin is a natural pterocarpan-type phytoalexin widely distributed in many traditional Chinese medicines, such as Astragali Radix. A previous study showed that Astragali Radix demonstrated promising protective effects in neurons. However, there is no reported study on the neuroprotective function and the underlying mechanism of Medicarpin. Read More

View Article and Full-Text PDF

Aurora kinase A (AURKA) promotes the progression and imatinib resistance of advanced gastrointestinal stromal tumors.

Cancer Cell Int 2021 Jul 31;21(1):407. Epub 2021 Jul 31.

Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, #88 Jiefang Road, Hangzhou, Zhejiang, People's Republic of China.

Background: Gastrointestinal stromal tumor (GIST) is a common tumor that originates from the alimentary system mesenchyme. Compared to typical gastrointestinal carcinomas, GISTs exhibit unique malignant behaviors. Bioinformatic tools and subsequent experiments were applied to investigate novel targets involved in GIST progression and imatinib resistance. Read More

View Article and Full-Text PDF

Computational identification of repurposed drugs against viruses causing epidemics and pandemics via drug-target network analysis.

Comput Biol Med 2021 Jul 23;136:104677. Epub 2021 Jul 23.

Virology Unit and Bioinformatics Centre, Institute of Microbial Technology, Council of Scientific and Industrial Research (CSIR), Sector 39-A, Chandigarh, 160036, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India. Electronic address:

Viral epidemics and pandemics are considered public health emergencies. However, traditional and novel antiviral discovery approaches are unable to mitigate them in a timely manner. Notably, drug repurposing emerged as an alternative strategy to provide antiviral solutions in a timely and cost-effective manner. Read More

View Article and Full-Text PDF

Incomplete information and irrelevant attributes in stated-preference values for health interventions.

Health Econ 2021 Jul 30. Epub 2021 Jul 30.

University of Calgary, Calgary, Alberta, Canada.

Violations of the assumptions of complete information [CI] and independence of irrelevant alternatives (IIA) in discrete-choice experiment (DCE) data imply sensitivity of preference estimates to the decision context and the alternatives evaluated. There is a paucity of evidence on how these two assumptions affect health-preference results and whether the usual specifications of random-parameters logit models are sufficient to address these violations. We assessed the appropriateness of these assumptions in a DCE valuating interventions to prevent long-term health problems that could be identified through whole genome sequencing. Read More

View Article and Full-Text PDF

Thermal Control Design and Packaging for Surface Acoustic Wave Devices in Acoustofluidics.

IEEE Trans Ultrason Ferroelectr Freq Control 2021 Jul 30;PP. Epub 2021 Jul 30.

This paper presents a thermal control design method for a surface acoustic wave (SAW) device. We designed a heat-dissipation structure and packaging scheme to solve three key issues observed in SAW devices using anisotropic crystals as piezoelectric substrates in acoustofluidics (e.g. Read More

View Article and Full-Text PDF

Glutamate Electropolymerization on Carbon Increases Analytical Sensitivity to Dopamine and Serotonin: An Auspicious Phenomenon in Mice?

Anal Chem 2021 Jul 30. Epub 2021 Jul 30.

Department of Chemistry and Biochemistry, University of South Carolina, Columbia, South Carolina, 29208 United States.

Carbon is the material of choice for electroanalysis of biological systems, being particularly applicable to neurotransmitter analysis as carbon fiber microelectrodes (CFMs). CFMs are most often applied to dopamine detection; however, the scope of CFM analysis has rapidly expanded over the last decade with our laboratory's focus being on improving serotonin detection at CFMs, which we achieved in the past Nafion modification. We began this present work by seeking to optimize this modification to gain increased analytical sensitivity toward serotonin under the assumption that exposure of bare carbon to the environment rapidly deteriorates analytical performance. Read More

View Article and Full-Text PDF

Modeling the structure and reactivity landscapes of a pyrazole-ammonium ionic derivative using wavefunction-dependent characteristics and screening for potential anti-inflammatory activity.

J Biomol Struct Dyn 2021 Jul 30:1-13. Epub 2021 Jul 30.

Department of Physics, Kanchi Shri Krishna College of Arts and Science, Kanchipuram, Tamil Nadu, India.

Spectroscopic investigations of 1-phenyl -2,3-dimethyl-5-oxo-1,2-dihydro-1-pyrazol-4-ammonium 2[(2-carboxyphenyl) disulfanyl]benzoate (PACB) reported experimentally and theoretically. NH-O interaction is observed and there is a very large downshift for NH-O stretching frequency. Reactive sites are identified from the chemical and electronic properties. Read More

View Article and Full-Text PDF

Sources and Fate of the Anti-Androgenic Fluorescent Dye 4-Methyl-7-Diethylaminocoumarin in Small River Systems.

Environ Toxicol Chem 2021 Jul 29. Epub 2021 Jul 29.

UFZ - Helmholtz Centre for Environmental Research, 04318, Leipzig, Germany.

Recently, the potent antiandrogen 4-methyl-7-diethylaminocoumarin (C47), and its potential transformation products 4-methyl-7-ethylaminocoumarin (C47T1) and 4-methyl-7-aminocoumarin (C47T2) were identified as novel environmental contaminants. We assessed for the first time the sources, distribution and fate of these compounds in aquatic systems using the Holtemme river (Saxony-Anhalt, Germany), which is a hotspot for these contaminants. To this end, wastewater treatment plant influent and effluent samples, surface water samples over three years and the longitudinal profiles in water, sediment and gammarids were analyzed. Read More

View Article and Full-Text PDF

Water quality thresholds for coastal contaminant impacts on corals: A systematic review and meta-analysis.

Sci Total Environ 2021 Jun 23;794:148632. Epub 2021 Jun 23.

Hawai'i Institute of Marine Biology, University of Hawai'i at Mānoa, 46-007 Lilipuna Road, Kāne'ohe, HI 96744, USA.

Reduced water quality degrades coral reefs, resulting in compromised ecosystem function and services to coastal communities. Increasing management capacity on reefs requires prioritization of the development of data-based water-quality thresholds and tipping points. To meet this urgent need of marine resource managers, we conducted a systematic review and meta-analysis that quantified the effects on scleractinian corals of chemical pollutants from land-based and atmospheric sources. Read More

View Article and Full-Text PDF

Rabbit haemorrhagic disease virus Lagovirus europaeus/GI.1d strain: genome sequencing, in vivo virus replication kinetics, and viral dose effect.

BMC Vet Res 2021 Jul 28;17(1):257. Epub 2021 Jul 28.

Ploufragan-Plouzané-Niort Laboratory, Avian and Rabbit Virology Immunology and Parasitology Unit, French Agency for Food, Environmental and Occupational Health and Safety (ANSES), BP 53, F-22440, Ploufragan, France.

Background: Rabbit haemorrhagic disease virus Lagovirus europaeus/GI.1d variant (GI.1d/RHDV) was identified in 1990 in France, and until the emergence of the new genotype GI. Read More

View Article and Full-Text PDF

Virus-induced Volatile Organic Compounds are Detectable in Exhaled Breath During Pulmonary Infection.

Am J Respir Crit Care Med 2021 Jul 28. Epub 2021 Jul 28.

Imperial College London, 4615, London, United Kingdom of Great Britain and Northern Ireland;

Background: Chronic obstructive pulmonary disease (COPD) is a condition punctuated by acute exacerbations commonly triggered by viral and/or bacterial infection. Early identification of exacerbation trigger is important to guide appropriate therapy but currently available tests are slow and imprecise. Volatile organic compounds (VOCs) can be detected in exhaled breath and have the potential to be rapid tissue-specific biomarkers of infection aetiology. Read More

View Article and Full-Text PDF

Molecular Routes of Dynamic Autocatalysis for Methanol-to-Hydrocarbons Reaction.

J Am Chem Soc 2021 Jul 28. Epub 2021 Jul 28.

National Engineering Laboratory for Methanol to Olefins, Dalian National Laboratory for Clean Energy, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, People's Republic of China.

The industrially important methanol-to-hydrocarbons (MTH) reaction is driven and sustained by autocatalysis in a dynamic and complex manner. Hitherto, the entire molecular routes and chemical nature of the autocatalytic network have not been well understood. Herein, with a multitechnique approach and multiscale analysis, we have obtained a full theoretical picture of the domino cascade of autocatalytic reaction network taking place on HZSM-5 zeolite. Read More

View Article and Full-Text PDF

Structural, Optical, and Electronic Properties of Two Quaternary Chalcogenide Semiconductors: AgSrSiS and AgSrGeS.

Inorg Chem 2021 Jul 28. Epub 2021 Jul 28.

Quaternary chalcogenide materials have long been a source of semiconductors for optoelectronic applications. Recent studies on I-II-IV-X (I = Ag, Cu, Li; II = Ba, Sr, Eu, Pb; IV = Si, Ge, Sn; X = S, Se) materials have shown particular versatility and promise among these compounds. These semiconductors take advantage of a diverse bonding scheme and chemical differences among cations to target a degree of antisite defect resistance. Read More

View Article and Full-Text PDF

Study of the Langat virus RNA-dependent RNA polymerase through homology modeling.

EMBnet J 2021 29;26(1). Epub 2021 Jun 29.

Laboratory of Genetics, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, Athens, Greece.

Langat virus is a member of the family and a close relative of a group of important tick-borne viruses that cause human encephalitis. RNA-dependent RNA polymerase is a significant component of the replication mechanism of the viral family. In the present work, a three-dimensional model of the Langat virus RNA-dependent RNA polymerase was designed through homology modeling. Read More

View Article and Full-Text PDF

The iron(III) and nickel(II) complexes with tetradentate thiosemicarbazones. Synthesis, experimental, theoretical characterization, and antiviral effect against SARS-CoV-2.

J Mol Struct 2021 Dec 22;1246:131166. Epub 2021 Jul 22.

Department of Chemistry, Engineering Faculty, Istanbul University-Cerrahpasa, 34320 Avcilar, Istanbul, Turkey.

The discovery of new inhibitors that can be used in the treatment of viral diseases, including Covid-19, is an area open to research, and there is a need for innovative compounds with increased efficiency that provide inhibition by suppressing enzyme, and receptor mechanisms. The iron(III) and nickel(II) complexes were synthesized by template condensation of 4-methoxy-salicylaldehyde with S-methylthiosemicarbazone derivatives of 1,1,1-trifluoroacetylacetone (for ) and methylacetoacetate (for ). The complex structures having NO-chelating thiosemicarbazidato ligand were identified by analytical, spectroscopic, and X-ray crystallography results. Read More

View Article and Full-Text PDF
December 2021

Identification of the miRNA signature and key genes in colorectal cancer lymph node metastasis.

Cancer Cell Int 2021 Jul 7;21(1):358. Epub 2021 Jul 7.

Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, No. 1055 San Xiang Road, Suzhou, 215004, Jiangsu, China.

Background: Because its metastasis to the lymph nodes are closely related to poor prognosis, miRNAs and mRNAs can serve as biomarkers for the diagnosis, prognosis, and therapy of colorectal cancer (CRC). This study aimed to identify novel gene signatures in the lymph node metastasis of CRC.

Methods: GSE56350, GSE70574, and GSE95109 datasets were downloaded from the Gene Expression Omnibus (GEO) database, while data from 569 colorectal cancer cases were also downloaded from The Cancer Genome Atlas (TCGA) database. Read More

View Article and Full-Text PDF

The pathology of natural and experimentally induced abortion in sheep.

J Vet Diagn Invest 2021 Jul 26:10406387211033293. Epub 2021 Jul 26.

Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.

We describe here the gross and microscopic lesions in 18 experimentally induced and 120 natural abortions. In natural abortions, gross lesions were reported infrequently; placentitis was recorded in 6% and hepatic lesions in 4% of our field cases. Placentitis was the microscopic lesion identified most consistently in natural abortions (93%) and was often observed in association with abundant bacterial colonies in chorionic villi (54%) and less often with placental vasculitis (13%). Read More

View Article and Full-Text PDF

Using multimodal chromatography for post-conjugation antibody-drug conjugate purification: A methodology from high throughput screening to in-silico process development.

J Chromatogr A 2021 Jun 30;1653:462378. Epub 2021 Jun 30.

Purification Process Sciences, BioPharmaceuticals Development, R&D, AstraZeneca, Gaithersburg, USA.

In this paper, a methodology for the development of a multimodal chromatography process is presented that is aimed at removal of under-conjugated antibody-drug conjugate (ADC) species. Two ADCs are used as case studies: One ADC results from site-directed conjugation to inserted cysteine residues and has a drug-to-antibody ratio (DAR) of two, the other is the product of conjugation to interchain disulfide bonds with a DAR of eight. First, filter plate screening studies are designed for the unconjugated antibody and the ADCs. Read More

View Article and Full-Text PDF

The 5-ketofructose reductase of sp. strain CHM43 is a novel class in the shikimate dehydrogenase family.

J Bacteriol 2021 Jul 26:JB0055820. Epub 2021 Jul 26.

Division of Life Science, Graduate School of Science and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8515, Japan.

sp. CHM43 oxidizes mannitol to fructose and then does fructose to 5-keto-D-fructose (5KF) in the periplasmic space. Since NADPH-dependent 5KF reductase was found in the soluble fraction of spp. Read More

View Article and Full-Text PDF

In vivo antimalarial activity of a probiotic bacterium Lactobacillus sakei isolated from traditionally fermented milk in BALB/c mice infected with Plasmodium berghei ANKA.

J Ethnopharmacol 2021 Jul 22;280:114448. Epub 2021 Jul 22.

Department of Biochemistry and Molecular Biology, Faculty of Science, University of Buea, Cameroon.

Ethnopharmacological Relevance: Milk production, processing and consumption are integral part of traditional practices in Fulani tribe of Cameroon. It has been observed that Fulani are resistant to malaria. Dairy products traditionally processed by Fulani are intensively used in the ritual treatment of malarial, inflammations and behavioural disorders. Read More

View Article and Full-Text PDF

Observing non-ergodicity due to kinetic constraints in tilted Fermi-Hubbard chains.

Nat Commun 2021 Jul 23;12(1):4490. Epub 2021 Jul 23.

Fakultät für Physik, Ludwig-Maximilians-Universität München, Munich, Germany.

The thermalization of isolated quantum many-body systems is deeply related to fundamental questions of quantum information theory. While integrable or many-body localized systems display non-ergodic behavior due to extensively many conserved quantities, recent theoretical studies have identified a rich variety of more exotic phenomena in between these two extreme limits. The tilted one-dimensional Fermi-Hubbard model, which is readily accessible in experiments with ultracold atoms, emerged as an intriguing playground to study non-ergodic behavior in a clean disorder-free system. Read More

View Article and Full-Text PDF