1,469 results match your criteria experimental sah

A High-Throughput Nanofluidic Device for Exosome Nanoporation to Develop Cargo Delivery Vehicles.

Small 2021 Jul 21:e2102150. Epub 2021 Jul 21.

Laboratory of Biomedical Microsystems and Nano Devices, Center for Bionic Sensing and Intelligence, Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.

Efficient loading of various exogenous cargos into exosomes while not affecting their integrity and functionalities remains a major challenge. Here, a nanofluidic device named "exosome nanoporator (ENP)" is presented for high-throughput loading of various cargos into exosomes. By transporting exosomes through nanochannels with height comparable to their dimension, exosome membranes are permeabilized by mechanical compression and fluid shear, allowing the influx of cargo molecules into the exosomes from the surrounding solution while maintaining exosome integrity. Read More

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Elevation of CSF Sortilin Following Subarachnoid Hemorrhage in Patients and Experimental Model Rats.

Neuroscience 2021 Jul 15. Epub 2021 Jul 15.

Department of Neurosurgery, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong 519000, China. Electronic address:

Subarachnoid hemorrhage (SAH) can cause acute neuronal injury and chronic neurocognitive deficits; biomarkers reflecting its associated neuronal injury are of potential prognostic value. Sortilin, a member of the vacuolar protein sorting 10p (Vps10p) family, is enriched in neurons and is likely involved in neurodegenerative diseases. Here, we explored sortilin in the cerebrospinal fluid (CSF) as a potential biomarker for early neuronal injury after SAH. Read More

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Infections in severe forms of alcohol-related liver disease - Need a closer look - Authors' reply.

Hepatology 2021 Jul 14. Epub 2021 Jul 14.

Department of Gastroenterology, Hepatopancreatology and Digestive Oncology, C.U.B. Hôpital Erasme, Université Libre de Bruxelles, Brussels, Belgium.

As already known, patients infected and successfully treated before initiation of corticosteroids have similar survival to non-infected sAH; thus, there is no reason to delay sAH treatment after infection control. As stated in the manuscript, 42(30%) patients were already infected upon admission. Read More

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Psychological distress after subarachnoid haemorrhage: A systematic review and meta-analysis.

J Psychosom Res 2021 Jul 4;148:110559. Epub 2021 Jul 4.

Clinical Neurosciences, Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Southampton, UK. Electronic address:

Objective: Psychological distress is a common complication in patients after Subarachnoid haemorrhage (SAH) which often has significant impact on the prognosis. The objective of this study was to determine the pooled prevalence of anxiety symptoms and depressive symptoms in patients after SAH and identify relevant risk factors.

Methods: The study adopted a systematic review and meta-analysis protocol. Read More

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Modulation of Salubrinal-mediated Endoplasmic Reticulum Stress in Experimental Subarachnoid Hemorrhage Model.

World Neurosurg 2021 Jul 7. Epub 2021 Jul 7.

Suleyman Demirel University, Faculty of Medicine, Department of Neurosurgery, Isparta, Turkey.

Background: Perfusion abnormalities due to vasospasm remain a major cause of morbidity and mortality in subarachnoid hemorrhage (SAH). Despite a large number of clinical trials, therapeutic options with strong evidence for prevention and treatment of cerebral vasospasm are rare. In this study, we aimed to evaluate the neuroprotective effect of Salubrinal (SLB) in endoplasmic stress-induced apoptosis, a catastrophic consequence of vasospasm. Read More

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Differential polarization and activation dynamics of systemic T helper cell subsets after aneurysmal subarachnoid hemorrhage (SAH) and during post-SAH complications.

Sci Rep 2021 Jul 9;11(1):14226. Epub 2021 Jul 9.

Department of Neurosurgery, University Hospital Bonn, University of Bonn, 53127, Bonn, Germany.

Aneurysmal subarachnoid hemorrhage (SAH) is associated with high morbidity and mortality. Devastating post-SAH complications, such as cerebral vasospasm (CVS), delayed cerebral ischemia or seizures to mention a few, are mainly responsible for the poor clinical outcome. Inflammation plays an indispensable role during early brain injury (EBI) and delayed brain injury (DBI) phases over which these complications arise. Read More

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Pre-Chiasmatic, Single Injection of Autologous Blood to Induce Experimental Subarachnoid Hemorrhage in a Rat Model.

J Vis Exp 2021 Jun 18(172). Epub 2021 Jun 18.

Department of Clinical Experimental Research, Glostrup Research Institute, Rigshospitalet.

Despite advances in treatment over the last decades, subarachnoid hemorrhage (SAH) continues to carry a high burden of morbidity and mortality, largely afflicting a fairly young population. Several animal models of SAH have been developed to investigate the pathophysiological mechanisms behind SAH and to test pharmacological interventions. The pre-chiasmatic, single injection model in the rat presented in this article is an experimental model of SAH with a predetermined blood volume. Read More

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Therapeutic effect of and mechanisms underlying the effect of miR-195-5p on subarachnoid hemorrhage-induced vasospasm and brain injury in rats.

PeerJ 2021 22;9:e11395. Epub 2021 Jun 22.

Division of Neurosurgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.

Objectives: There is much evidence suggesting that inflammation contributes majorly to subarachnoid hemorrhage (SAH)-induced cerebral vasospasm and brain injury. miRNAs have been found to modulate inflammation in several neurological disorders. This study investigated the effect of miR-195-5p on SAH-induced vasospasm and early brain injury in experimental rats. Read More

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Role of Anesthetics and Their Adjuvants in Neurovascular Protection in Secondary Brain Injury after Aneurysmal Subarachnoid Hemorrhage.

Int J Mol Sci 2021 Jun 18;22(12). Epub 2021 Jun 18.

Department of Neurological Surgery, Washington University, St. Louis, MO 63110, USA.

Aneurysmal rupture accounts for the majority of subarachnoid hemorrhage and is responsible for most cerebrovascular deaths with high mortality and morbidity. Initial hemorrhage severity and secondary brain injury due to early brain injury and delayed cerebral ischemia are the major determinants of outcomes after aneurysmal subarachnoid hemorrhage. Several therapies have been explored to prevent these secondary brain injury processes after aneurysmal subarachnoid hemorrhage with limited clinical success. Read More

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RelA-SpoT Homolog toxins pyrophosphorylate the CCA end of tRNA to inhibit protein synthesis.

Mol Cell 2021 Jun 23. Epub 2021 Jun 23.

Department of Experimental Medical Science, Lund University, 221 00 Lund, Sweden; University of Tartu, Institute of Technology, 50411 Tartu, Estonia; Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå University, 901 87 Umeå, Sweden. Electronic address:

RelA-SpoT Homolog (RSH) enzymes control bacterial physiology through synthesis and degradation of the nucleotide alarmone (p)ppGpp. We recently discovered multiple families of small alarmone synthetase (SAS) RSH acting as toxins of toxin-antitoxin (TA) modules, with the FaRel subfamily of toxSAS abrogating bacterial growth by producing an analog of (p)ppGpp, (pp)pApp. Here we probe the mechanism of growth arrest used by four experimentally unexplored subfamilies of toxSAS: FaRel2, PhRel, PhRel2, and CapRel. Read More

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Analysis of Cerebral Vasospasm in a Murine Model of Subarachnoid Hemorrhage with High Frequency Transcranial Duplex Ultrasound.

J Vis Exp 2021 Jun 3(172). Epub 2021 Jun 3.

Department of Anesthesiology, University Medical Center of the Johannes Gutenberg-University of Mainz.

Cerebral vasospasm that occurs in the weeks after subarachnoid hemorrhage, a type of hemorrhagic stroke, contributes to delayed cerebral ischemia. A problem encountered in experimental studies using murine models of SAH is that methods for in vivo monitoring of cerebral vasospasm in mice are lacking. Here, we demonstrate the application of high frequency ultrasound to perform transcranial Duplex sonography examinations on mice. Read More

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Ponesimod protects against neuronal death by suppressing the activation of A1 astrocytes in early brain injury after experimental subarachnoid hemorrhage.

J Neurochem 2021 Jun 18. Epub 2021 Jun 18.

Department of Neurosurgery, The Affiliated Hospital of Southwest Medical University, Luzhou, China.

As an important initiator and responder of brain inflammation in the central nervous system (CNS), astrocytes transform into two new reactive phenotypes with changed morphology, altered gene expression and secretion profiles, termed detrimental A1 and beneficial A2. Inflammatory events have been shown to occur during the phase of early brain injury (EBI) after subarachnoid hemorrhage (SAH). However, the phenotype transformation of astrocytes as well as its potential contribution to inflammatory status in the EBI of SAH has yet to be determined. Read More

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Role of platelets in the pathogenesis of delayed injury after subarachnoid hemorrhage.

J Cereb Blood Flow Metab 2021 Jun 10:271678X211020865. Epub 2021 Jun 10.

The Vivian L. Smith Department of Neurosurgery, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX, USA.

Aneurysmal subarachnoid hemorrhage (aSAH) patients develop delayed cerebral ischemia and delayed deficits (DCI) within 2 weeks of aneurysm rupture at a rate of approximately 30%. DCI is a major contributor to morbidity and mortality after SAH. The cause of DCI is multi-factorial with contributions from microthrombi, blood vessel constriction, inflammation, and cortical spreading depolarizations. Read More

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Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus.

J Vis Exp 2021 May 13(171). Epub 2021 May 13.

Department of Immunotherapeutics and Biotechnology, Jerry H. Hodge School of Pharmacy, Texas Tech University Health Sciences Center;

Oncolytic viruses (OVs), such as the oncolytic herpes simplex virus (oHSV), are a rapidly growing treatment strategy in the field of cancer immunotherapy. OVs, including oHSV, selectively replicate in and kill cancer cells (sparing healthy/normal cells) while inducing anti-tumor immunity. Because of these unique properties, oHSV-based treatment strategies are being increasingly used for the treatment of cancer, preclinically and clinically, including FDA-approved talimogene laherparevec (T-Vec). Read More

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Statins Improve Clinical Outcome After Non-aneurysmal Subarachnoid Hemorrhage: A Translational Insight From a Systematic Review of Experimental Studies.

Front Neurol 2021 14;12:620096. Epub 2021 May 14.

Department of Neurosurgery, University Hospital Frankfurt, Frankfurt am Main, Germany.

The efficacy of statin-treatment in aneurysmal subarachnoid hemorrhage (SAH) remains controversial. We aimed to investigate the effects of statin-treatment in non-aneurysmal (na)SAH in accordance with animal research data illustrating the pathophysiology of naSAH. We systematically searched PubMed using PRISMA-guidelines and selected experimental studies assessing the statin-effect on SAH. Read More

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Clinical course and risk factors for infection in severe forms of alcohol-related liver disease.

Hepatology 2021 May 28. Epub 2021 May 28.

Department of Gastroenterology, Hepatopancreatology and Digestive Oncology, CUB Hôpital Erasme, Université Libre de Bruxelles, Brussels, Belgium.

Background & Aims: Infection is a major driver of mortality in patients with advanced alcohol-related liver disease (ALD). The epidemiology and clinical course of infected patients with life-threatening forms of ALD, including severe alcoholic hepatitis (sAH) and decompensated alcoholic cirrhosis (DAC), and specific risk factors for infection remain mostly unknown.

Approach & Results: In this observational study, we assessed all infectious episodes occurring within a 90-day period from diagnosis in all consecutive patients with biopsy-proven sAH (mDF≥32, MELD≥18) and DAC (MELD≥18), without alcoholic hepatitis, in our tertiary hospital between 2003 and 2016. Read More

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Levosimendan as a therapeutic strategy to prevent neuroinflammation after aneurysmal subarachnoid hemorrhage?

J Neurointerv Surg 2021 May 26. Epub 2021 May 26.

Neurosurgery, Goethe University Hospital, Frankfurt am Main, Germany.

Background: Poor patient outcomes after aneurysmal subarachnoid hemorrhage (SAH) occur due to a multifactorial process, mainly involving cerebral inflammation (CI), delayed cerebral vasospasm (DCVS), and delayed cerebral ischemia, followed by neurodegeneration. CI is mainly triggered by enhanced synthesis of serotonin (5-HT), prostaglandin F2alpha (PGF2a), and cytokines such as interleukins. Levosimendan (LV), a calcium-channel sensitizer, has already displayed anti-inflammatory effects in patients with severe heart failure. Read More

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Acute micro-thrombosis after subarachnoid hemorrhage: A new therapeutic target?

J Cereb Blood Flow Metab 2021 May 17:271678X211013595. Epub 2021 May 17.

Department of Neurosurgery, University of Michigan, Ann Arbor, MI, USA.

Microthrombi formation in the brain following subarachnoid hemorrhage (SAH) has been recognized and suspected to contribute to cerebral ischemia. A recent study found that ultra-early cerebral micro-thrombosis occured four hours after experimental SAH. The number of thrombotic microvessels correlated with brain-blood barrier disruption and neuronal injury. Read More

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MFGE8 mitigates brain injury in a rat model of SAH by maintaining vascular endothelial integrity via TIGβ5/PI3K/CXCL12 signaling.

Exp Brain Res 2021 Jul 15;239(7):2193-2205. Epub 2021 May 15.

Department of Neurosurgery, The Fifth Affiliated Hospital of Sun Yat-sen University, No. 52 Meihuadong Road, Zhuhai, 519000, Guangdong, China.

Leaked blood components, injured endothelial cells, local inflammatory response and vasospasm may converge to promote microthrombosis following subarachnoid hemorrhage (SAH). Previously, we showed that the milk fat globule-epidermal growth factor 8 (MFGE8) can mitigate SAH-induced microthrombosis. This present study was aimed to explore the molecular pathway participated in MFGE8-dependent protection on vascular endothelium. Read More

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Effect of freshly placed core buildup composites on setting of silicon impression materials.

J Indian Prosthodont Soc 2021 Apr-Jun;21(2):186-191

Department of Industrial Engineering, The University of Jordan, Amman, Jordan.

Aim: The aim is to study the effect of freshly placed composite build-ups on setting of additional silicone impression materials.

Settings And Design: In vitro - experimental study.

Materials And Methods: Three composite materials; Build-It™ F. Read More

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The calcimimetic R-568 attenuates subarachnoid hemorrhage-induced vasospasm through PI3K/Akt/eNOS signaling pathway in the rat model.

Brain Res 2021 Aug 28;1765:147508. Epub 2021 Apr 28.

Health Sciences University, Bağcılar Training and Research Hospital, Neurosurgery Clinic, Istanbul, Turkey. Electronic address:

Cerebral vasospasm (CVS) causes mortality and morbidity in patients after subarachnoid hemorrhage (SAH). The mechanism and adequate treatment of CVS are still elusive. R-568 is a calcimimetic agent known to exert a vasodilating effect. Read More

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Hydrocephalus Following Experimental Subarachnoid Hemorrhage in Rats with Different Aerobic Capacity.

Int J Mol Sci 2021 Apr 26;22(9). Epub 2021 Apr 26.

Department of Neurosurgery, University of Michigan, Ann Arbor, MI 48109, USA.

Low aerobic capacity is considered to be a risk factor for stroke, while the mechanisms underlying the phenomenon are still unclear. The current study looked into the impacts of different aerobic capacities on early brain injury in a subarachnoid hemorrhage (SAH) model using rats bred for high and low aerobic capacity (high-capacity runners, HCR; low-capacity runners, LCR). SAH was modeled with endovascular perforation in HCR and LCR rats. Read More

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Role of SIRT1 in Isoflurane Conditioning-Induced Neurovascular Protection against Delayed Cerebral Ischemia Secondary to Subarachnoid Hemorrhage.

Int J Mol Sci 2021 Apr 20;22(8). Epub 2021 Apr 20.

Department of Anesthesiology, Washington University in Saint Louis, Saint Louis, MO 63110, USA.

We recently reported that isoflurane conditioning provided multifaceted protection against subarachnoid hemorrhage (SAH)-induced delayed cerebral ischemia (DCI), and this protection was through the upregulation of endothelial nitric oxide synthase (eNOS). SIRT1, an NAD-dependent deacetylase, was shown to be one of the critical regulators of eNOS. The aim of our current study is to examine the role of SIRT1 in isoflurane conditioning-induced neurovascular protection against SAH-induced DCI. Read More

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Blockade of Motor Cortical Long-Term Potentiation Induction by Glutamatergic Dysfunction Causes Abnormal Neurobehavior in an Experimental Subarachnoid Hemorrhage Model.

Front Neural Circuits 2021 9;15:670189. Epub 2021 Apr 9.

Department of Neurosurgery, School of Medicine, Oita University, Oita, Japan.

Subarachnoid hemorrhage (SAH) is a life-threatening condition that can also lead to permanent paralysis. However, the mechanisms that underlying neurobehavioral deficits after SAH have not been fully elucidated. As theta burst stimulation (TBS) can induce long-term potentiation (LTP) in the motor cortex, we tested its potential as a functional evaluation tool after experimentally induced SAH. Read More

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Concentrations of estradiol, progesterone and testosterone in sefrum and cerebrospinal fluid of patients with aneurysmal subarachnoid hemorrhage correlate weakly with transcranial Doppler flow velocities.

BMC Neurosci 2021 04 23;22(1):29. Epub 2021 Apr 23.

Department of Anesthesiology and Intensive Care Medicine, School of Medicine, Klinikum Rechts Der Isar, Technical University of Munich, Ismaninger Str. 22, 81675, Munich, Germany.

Background: The implication of the steroids estradiol, progesterone and testosterone in cerebral vasospasm after aneurysmal subarachnoid hemorrhage (aSAH) has not been comprehensively assessed. In rodents, studies suggested beneficial effects of steroids on cerebral vasospasm after experimental SAH. Studies in humans are warranted, however, a general dilemma of human studies on neuroactive substances is that the brain is not directly accessible and that concentrations in the periphery may not adequately parallel concentrations in the central compartments. Read More

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An Enriched Environment Leads to Increased Synaptic Plasticity-Associated miRNA Levels after Experimental Subarachnoid Hemorrhage.

J Stroke Cerebrovasc Dis 2021 Jun 16;30(6):105766. Epub 2021 Apr 16.

Department of Interdisciplinary Neuroscience, Health Science Institute, Eskisehir Osmangazi University, Eskisehir, Turkey; Department of Neurosurgery, Faculty of Medicine, Eskisehir Osmangazi University, Eskisehir, Turkey.

Background And Purpose: In subarachnoid hemorrhage (SAH), impairments in motor and cognitive functions may occur and continue in later periods. MicroRNAs (miRNAs) are small non-coding RNAs that can directly or indirectly affect synaptic reconstruction. mir-132, mir-134, and mir-138 are the leading miRNAs that can be effective on some neurological functions through its effects on synaptic plasticity in the relevant brain areas. Read More

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Gene expression profiling of brain endothelial cells after experimental subarachnoid haemorrhage.

Sci Rep 2021 Apr 9;11(1):7818. Epub 2021 Apr 9.

Division of Neurosurgery, St. Michael's Hospital, Keenan Research Centre for Biomedical Science and the Li Ka Shing Knowledge Institute of St. Michael's Hospital, University of Toronto, Toronto, ON, Canada.

Subarachnoid haemorrhage (SAH) is a type of hemorrhagic stroke that is associated with high morbidity and mortality. New effective treatments are needed to improve outcomes. The pathophysiology of SAH is complex and includes early brain injury and delayed cerebral ischemia, both of which are characterized by blood-brain barrier (BBB) impairment. Read More

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Clarithromycin Ameliorates Early Brain Injury After Subarachnoid Hemorrhage via Suppressing Periostin-Related Pathways in Mice.

Neurotherapeutics 2021 Apr 7. Epub 2021 Apr 7.

Department of Neurosurgery, Mie University Graduate School of Medicine, Tsu, Japan.

Subarachnoid hemorrhage (SAH) remains a life-threatening disease, and early brain injury (EBI) is an important cause of poor outcomes. The authors have reported that periostin, a matricellular protein, is one of key factors of post-SAH EBI. Clarithromycin (CAM) is a worldwide antibiotic that can inhibit periostin expression. Read More

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Exploring the main and moderating effects of individual-level characteristics on consumer responses to sugar taxes and front-of-pack nutrition labels in an experimental marketplace.

Can J Public Health 2021 Aug 25;112(4):647-662. Epub 2021 Mar 25.

School of Public Health and Health Systems, University of Waterloo, 200 University Ave W., Waterloo, ON, N2L 3G1, Canada.

Intervention: This study examined whether the impacts of sugar taxes and front-of-pack (FOP) nutrition labels differ across socio-demographic subgroups.

Research Question: What are the main and moderating effects of individual-level characteristics on the nutrient content of participants' purchases in response to varying taxation levels and FOP labels?

Methods: Data from an experimental marketplace were analyzed. A sample of 3584 Canadians aged 13 years and older received $5 to purchase an item from a selection of 20 beverages and 20 snack foods. Read More

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First emerging evidence of the relationship between Onuf's nucleus degeneration and reduced sperm number following spinal subarachnoid haemorrhage: Experimental study.

Andrologia 2021 Jun 23;53(5):e14030. Epub 2021 Mar 23.

Department of Urology, Medical Faculty of Recep Tayyip Erdogan University, Rize, Turkey.

Lumbosacral pathologies can lead to infertility. Onuf's nucleus changes in these pathologies may have a role in low sperm number. This study aims to investigate the relationship between Onuf's nucleus degeneration and sperm number following spinal subarachnoid haemorrhage. Read More

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