21 results match your criteria existence gelling

  • Page 1 of 1

Recombinant transgelin-like protein 1 from Mytilus shell induces formation of CaCO polymorphic crystals in vitro.

FEBS Open Bio 2020 Oct 21;10(10):2216-2234. Epub 2020 Sep 21.

Laboratory of Marine Biology Protein Engineering, Marine Science and Technical College, Zhejiang Ocean University, Zhoushan City, China.

Transgelin is an actin cross-linking/gelling protein of the calponin family, which is associated with actin stress fibres, cell motility, adhesion and the maintenance of cell morphology. Transgelin-like proteins (TLPs) have also been identified as shell matrix proteins (SMPs) in several mollusc species; however, the functions of TLPs in biomineralization remain unknown. Transgelin-like protein 1 (TLP-1) was previously identified from the shell of Mytilus coruscus as a novel 19 kDa SMP with a calponin homology (CH) domain. Read More

View Article and Full-Text PDF
October 2020

Rheological characteristics and supramolecular structure of the exopolysaccharide produced by Lactobacillus fermentum MTCC 25067.

Carbohydr Polym 2019 Aug 27;218:226-233. Epub 2019 Apr 27.

Research Center for Global Agromedicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan. Electronic address:

Rheological properties and supramolecular structure of the exopolysaccharide (EPS) secreted by Lactobacillus fermentum MTCC 25067 were investigated. The critical concentration representing the lower-limit of the semi-dilute regime was estimated to be 0.71 g/L from the concentration dependence of zero-shear specific viscosity. Read More

View Article and Full-Text PDF

Antioxidant pectin enriched fractions obtained from discarded carrots (Daucus carota L.) by ultrasound-enzyme assisted extraction.

Food Chem 2019 Aug 18;289:453-460. Epub 2019 Mar 18.

Departamento de Industrias, ITAPROQ (CONICET-UBA), Facultad de Ciencias Exactas y Naturales, University of Buenos Aires, Ciudad Universitaria, C1428BGA Buenos Aires, Argentina. Electronic address:

Carrot residues were upgraded as pectin-enriched fractions (PEFs) useful for functional food formulation due to co-extracted antioxidants (α- and β-carotenes, lutein, α-tocopherol), and gelling effect. High power ultrasound (US)-enzyme assisted extraction was applied for efficiency and sustainability. Carrot powder (CP) in citrate-buffer (pH 5. Read More

View Article and Full-Text PDF

Negative Additive Manufacturing of Complex Shaped Boron Carbides.

J Vis Exp 2018 09 18(139). Epub 2018 Sep 18.

Lawrence Livermore National Laboratory.

Boron carbide (B4C) is one of the hardest materials in existence. However, this attractive property also limits its machineability into complex shapes for high wear, high hardness, and lightweight material applications such as armors. To overcome this challenge, negative additive manufacturing (AM) is employed to produce complex geometries of boron carbides at various length scales. Read More

View Article and Full-Text PDF
September 2018

Fabrication of an ionic-sensitive in situ gel loaded with resveratrol nanosuspensions intended for direct nose-to-brain delivery.

Colloids Surf B Biointerfaces 2016 Nov 20;147:376-386. Epub 2016 Aug 20.

College of Pharmacy, Taishan Medical University, Taian 271016, PR China. Electronic address:

The objective of this study was to fabricate a composite in situ gelling formulation combining nanoparticulates and an ionic-triggered deacetylated gellan gum (DGG) matrix for challenging intranasal drug delivery. The prepared resveratrol nanosuspensions (Res-NSs) were distributed in DGG solutions. Parameters such as the in situ gelation capability, particle size, rheological properties, and texture profiles were used to describe the properties of the in situ gel. Read More

View Article and Full-Text PDF
November 2016

Lenghty reverse poly(butylene oxide)-poly(ethylene oxide)-poly(butylene oxide) polymeric micelles and gels for sustained release of antifungal drugs.

Int J Pharm 2016 Aug 8;510(1):17-29. Epub 2016 Jun 8.

Grupo de Física de Coloides y Polímeros, Departamento de Física de la Materia Condensada; Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain. Electronic address:

In this work, we present a detailed study of the potential application of polymeric micelles and gels of four different reverse triblock poly(butylene oxide)-poly(ethylene oxide)-poly(butylene oxide) copolymers (BOnEOmBOn, where n denotes the respective block lengths), specifically BO8EO90BO8, BO14EO378BO14, BO20EO411BO20 and BO21EO385BO21, as effective drug transport nanocarriers. In particular, we tested the use of this kind of polymeric nanostructures as reservoirs for the sustained delivery of the antifungals griseofulvin and fluconazole for oral and topical administration. Polymeric micelles and gels formed by these copolymers were shown to solubilize important amounts of these two drugs and to have a good stability in physiologically relevant conditions for oral or topical administration. Read More

View Article and Full-Text PDF

Supramolecular Gels by Design: Towards the Development of Topical Gels for Self-Delivery Application.

Chemistry 2016 Jun 25;22(27):9257-66. Epub 2016 May 25.

Department of Organic Chemistry, Indian Association for the Cultivation of Science, 2A & 2B Raja S.C. Mullick Road, Kolkata, 700032, West Bengal, India.

Following a supramolecular synthon approach, simple salt formation has been employed to gain access to a series of supramolecular gelators derived from the well-known non-steroidal anti-inflammatory drug (NSAID) ibuprofen. A well-studied gel-inducing supramolecular synthon, namely primary ammonium monocarboxylate (PAM), has been exploited to generate a series of PAM salts by reacting ibuprofen with various primary amines. Remarkably, all of the salts (S1-S7) thus synthesized proved to be good to moderate gelators of various polar and nonpolar solvents. Read More

View Article and Full-Text PDF

Gelation of wheat arabinoxylans in the presence of Cu(+2) and in aqueous mixtures with cereal β-glucans.

Food Chem 2016 Jul 10;203:267-275. Epub 2016 Feb 10.

Laboratory of Food Chemistry & Biochemistry, Department of Food Science and Technology, School of Agriculture, Aristotle University, Greece. Electronic address:

The effect of copper ions (0.63-0.16 mM) on the rheological behavior of arabinoxylan (AX) aqueous solutions was investigated. Read More

View Article and Full-Text PDF

Enzyme-Assisted Preparation of Furcellaran-Like κ-/β-Carrageenan.

Mar Biotechnol (NY) 2016 Feb 19;18(1):133-43. Epub 2015 Nov 19.

Sorbonne Universités, UPMC Univ Paris 06, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, 29680, Roscoff, France.

Carrageenans are sulfated galactans that are widely used in industrial applications for their thickening and gelling properties, which vary according to the amount and distribution of ester sulfate groups along the galactan backbone. To determine and direct the sulfation of κ-carrageenan moieties, we purified an endo-κ-carrageenan sulfatase (Q15XH1 accession in UniprotKB) from Pseudoalteromonas atlantica T6c extracts. Based on sequence analyses and exploration of the genomic environment of Q15XH1, we discovered and characterized a second endo-κ-carrageenan sulfatase (Q15XG7 accession in UniprotKB). Read More

View Article and Full-Text PDF
February 2016

Exploiting supramolecular synthons in designing gelators derived from multiple drugs.

Chemistry 2014 Nov 15;20(47):15320-4. Epub 2014 Oct 15.

Department of Organic Chemistry, Indian Association for the Cultivation of Science (IACS), 2A and 2B, Raja S. C. Mullick Road, Jadavpur, Kolkata - 700032, West Bengal (India), Fax: (+91) 33-2473-2805.

A simple strategy for designing salt-based supramolecular gelators comprised of various nonsteroidal anti-inflammatory drugs (NSAIDs) and amantadine (AMN) (an antiviral drug) has been demonstrated using a supramolecular synthon approach. Single-crystal and powder X-ray diffraction established the existence of the well-studied gel-forming 1D supramolecular synthon, namely, primary ammonium monocarboxylate (PAM) synthon in all the salts. Remarkably five out of six salts were found to be capable of gelling methyl salicylate (MS)-an important ingredient in commercially available topical gels; one such selected biocompatible salt displayed an anti-inflammatory response in prostaglandin E2 (PGE2 ) assay, thereby indicating their plausible biomedical applications. Read More

View Article and Full-Text PDF
November 2014

Liquid crystalline phase formation in suspensions of solid trimyristin nanoparticles.

J Phys Chem B 2014 Sep 15;118(38):11387-96. Epub 2014 Sep 15.

Physik Department, Friedrich-Alexander-Universität Erlangen-Nürnberg , Staudtstrasse 3, 91058 Erlangen, Germany.

The presence of liquid crystalline phases in suspensions of solid lipid nanoparticles can increase the risk of their gelling upon administration through fine needles. Here we study the formation of liquid crystalline phases in aqueous suspensions of platelet-like shaped solid lipid nanoparticles. A native lecithin-stabilized trimyristin (20 wt %) suspension was investigated at different dilution levels by small-angle X-ray scattering (SAXS) and visual inspection of their birefringence between two crossed polarizers. Read More

View Article and Full-Text PDF
September 2014

Ternary phase diagrams of a thermoreversible chelating non-ionic surfactant.

Phys Chem Chem Phys 2009 Apr 25;11(15):2700-7. Epub 2009 Feb 25.

CEA Saclay, DSM/IRAMIS/SCM/LIONS, 91191, Gif sur Yvette cedex, France.

The behaviour of a di-block molecule associating a diamide group and a non-ionic surfactant (C(i)E(j)) is determined in a ternary surfactant/water/oil/system. Its properties are compared to the ones of a parent non-ionic surfactant C(i)E(j) through the limits of boundaries in the phase prisms. The existence of a stable microemulsion single phase in a defined temperature and concentration range is demonstrated. Read More

View Article and Full-Text PDF

Microemulsion-based vaginal gel of clotrimazole: formulation, in vitro evaluation, and stability studies.

AAPS PharmSciTech 2009 21;10(2):476-81. Epub 2009 Apr 21.

Department of Pharmaceutical Sciences and Technology, Institute Chemical Technology, Matunga, Mumbai, 400019, India.

The objective of the present investigation was to develop and evaluate microemulsion-based gel for the vaginal delivery of clotrimazole (CMZ). The solubility of CMZ in oils and surfactants was evaluated to identify components of the microemulsion. The ternary diagram was plotted to identify the area of microemulsion existence. Read More

View Article and Full-Text PDF

Essential role of one-carbon metabolism and Gcn4p and Bas1p transcriptional regulators during adaptation to anaerobic growth of Saccharomyces cerevisiae.

J Biol Chem 2009 Apr 18;284(17):11205-15. Epub 2009 Feb 18.

School of Biomedical and Health Sciences, University of Western Sydney, Penrith, New South Wales DC 1791, Australia.

The transcriptional activator Gcn4p is considered the master regulator of amino acid metabolism in Saccharomyces cerevisiae and is required for the transcriptional response to amino acid starvation. Here it is shown that Gcn4p plays a previously undescribed role in regulating adaptation to anaerobic growth. A gcn4 mutant exhibited a highly extended lag phase after a shift to anaerobiosis that was the result of l-serine depletion. Read More

View Article and Full-Text PDF

Microemulsion based vaginal gel of fluconazole: formulation, in vitro and in vivo evaluation.

Int J Pharm 2009 Jan 23;365(1-2):175-9. Epub 2008 Aug 23.

Department of Pharmaceutical Sciences and Technology, Institute Chemical Technology, Matunga, Mumbai 400019, India.

The objective of the present investigation was to develop and evaluate microemulsion based gel for the vaginal delivery of fluconazole (FLZ). The solubility of FLZ in oils and surfactants was evaluated to identify components of the microemulsion. The ternary diagram was plotted to identify the area of microemulsion existence. Read More

View Article and Full-Text PDF
January 2009

Increased lipid oxidation heralds diabetes onset in DR.lyp/lyp rats.

Exp Clin Endocrinol Diabetes 2008 Aug 9;116(8):475-80. Epub 2008 May 9.

Department of Clinical Sciences, Clinical Research Center, Lund University, Malmö, Sweden.

Aim: The BB rat model of type 1 diabetes exhibits altered body weight gain and body temperature regulation prior to hyperglycemia onset, implying the existence of as yet unidentified biomarkers of autoimmune processes that destroy pancreatic beta cells. To investigate this hypothesis, we compared the metabolic profile of diabetes-resistant DR.lyp/+ rats and their diabetes-prone, congenic DR. Read More

View Article and Full-Text PDF

The pH threshold in the dissolution of beta-lactoglobulin gels and aggregates in alkali.

Biomacromolecules 2007 Apr 23;8(4):1162-70. Epub 2007 Mar 23.

Department of Chemical Engineering, New Museums Site, Pembroke Street, Cambridge, CB2 3RA, UK.

The existence of a practical minimum pH for the dissolution of heat-induced whey gels in alkaline solutions has been studied using beta-lactoglobulin (betaLg) as a model protein. A sharp transition in solubility was observed between pH 11 and 12; this transition shifts to higher pHs for gels formed at higher temperatures and for longer gelling times. The breakdown reactions of heat-induced aggregates in alkali were monitored with size exclusion chromatography. Read More

View Article and Full-Text PDF

SMGA gels for the skin permeation of haloperidol.

J Control Release 2005 Aug;106(1-2):88-98

Department of Pharmacy, National University of Singapore, Republic of Singapore.

Small molecule gelling agent (SMGA) gels were developed using the gelator GP-1 in the solvents, namely, isostearyl alcohol (ISA) and propylene glycol (PG), to deliver haloperidol through the skin. The concentrations of the drug, haloperidol, the enhancer, farnesol and the gelator, GP-1 are 3 mg/ml, 5% (w/v) and 5% (w/v), respectively. The study employed a three-factor full factorial statistical design to investigate the influence of factor level changes on the permeability coefficient and permeation lag-time of haloperidol. Read More

View Article and Full-Text PDF

Glucose-dependent insulinotropic polypeptide stimulation of lipolysis in differentiated 3T3-L1 cells: wortmannin-sensitive inhibition by insulin.

Endocrinology 1999 Jan;140(1):398-404

Department of Physiology, University of British Columbia, Vancouver, Canada.

GIP is an important insulinotropic hormone (incretin) that has also been implicated in fat metabolism. There is controversy regarding the actions of GIP on adipocytes. In the current study, the existence of GIP receptors and effects of GIP on lipolysis were studied in differentiated 3T3-L1 cells. Read More

View Article and Full-Text PDF
January 1999

Structure-function relationships in microbial exopolysaccharides.

Authors:
I W Sutherland

Biotechnol Adv 1994 ;12(2):393-448

Institute of Cell and Molecular Biology, Division of Biology, University of Edinburgh, Scotland.

Sufficient well-characterized microbial exopolysaccharides are now available to permit extensive studies on the relationship between their chemical structure and their physical attributes. This is seen even in homopolysaccharides with relatively simple structures but is more marked when greater differences in structure exist, as are found in several heteropolysaccharides. The specific and sometimes unique properties have, in the case of several of these polymers, provided a range of commercial applications. Read More

View Article and Full-Text PDF
December 2003

Electrophoretic size separations in liquified agarose of polystyrene particles and circular DNA.

Authors:
P Bocek A Chrambach

Electrophoresis 1991 Sep;12(9):620-3

Section on Macromolecular Analysis, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-0001.

Polystyrene sulfate particles of 0.37 to 1.78 mu in diameter are retarded in their electrophoretic migration in proportion to the concentration of agarose liquified above its gelling temperature. Read More

View Article and Full-Text PDF
September 1991
  • Page 1 of 1