141,753 results match your criteria exclusion chromatography

Evaluating Directional Dependency of Selective Laser Sintered Patient Specific Biodegradable Devices to Improve Predictive Modeling and Design Verification.

Ann Biomed Eng 2021 Jul 21. Epub 2021 Jul 21.

Department of Biomedical Engineering and Center for 3D Medical Fabrication (3DMedFab), Georgia Institute of Technology, Atlanta, GA, 30332, USA.

Additive manufacturing, or 3D printing, of the bioresorbable polymer [Formula: see text]-polycaprolactone (PCL) is an emerging tissue engineering solution addressing patient specific anatomies. Predictively modeling the mechanical behavior of 3D printed parts comprised of PCL improves the ability to develop patient specific devices that meet design requirements while reducing the testing of extraneous design variants and development time for emergency devices. Predicting mechanical behavior of 3D-printed devices is limited by the variability of effective material moduli that are determined in part by the 3D printing manufacturing process. Read More

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Biochemical and Phylogenetic Characterization of a Novel NADP-Specific Isocitrate Dehydrogenase From the Marine Microalga .

Front Mol Biosci 2021 5;8:702083. Epub 2021 Jul 5.

Anhui Provincial Key Laboratory of Molecular Enzymology and Mechanism of Major Diseases, Key Laboratory of Biomedicine in Gene Diseases and Health of Anhui Higher Education Institutes, College of Life Sciences, Anhui Normal University, Wuhu, China.

Isocitrate dehydrogenase (IDH) family of proteins is classified into three subfamilies, namely, types I, II, and III. Although IDHs are widely distributed in bacteria, archaea, and eukaryotes, all type III IDHs reported to date are found only in prokaryotes. Herein, a novel type III IDH subfamily member from the marine microalga (PtIDH2) was overexpressed, purified, and characterized in detail for the first time. Read More

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Structural Features of Cork Dioxane Lignin from L.

J Agric Food Chem 2021 Jul 21. Epub 2021 Jul 21.

CICECO, Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.

The dioxane lignin was isolated from extractives- and suberin-free cork ( L.) by a modified acidolytic procedure and submitted to structural analysis by permanganate oxidation, analytical pyrolysis coupled with gas chromatography and mass spectrometry (Py-GC-MS), liquid- and solid-state nuclear magnetic resonance (NMR) spectroscopy, and Fourier transform infrared (FTIR) spectroscopy. The molecular weight ( = 2500 Da) was assessed by size exclusion chromatography (SEC). Read More

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Purification and Characterization of a New Halocin HA4 from Haloferax larsenii HA4 Isolated from a Salt Lake.

Probiotics Antimicrob Proteins 2021 Jul 20. Epub 2021 Jul 20.

Department of Genetics, Maharshi Dayanand University, Rohtak, 124001, Haryana, India.

Halocins are antimicrobial peptides secreted by different members of haloarchaea. Halocin HA4 was purified from Haloferax larsenii HA4 using combination of ultrafiltration and chromatographic techniques. It was found to be ~ 14 kDa with unique N-terminal sequence, HN-AEEEIFXPDX, which did not show homology with the known sequence suggesting a new/novel compound. Read More

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Purification of RNA Mango Tagged Native RNA-protein Complexes from Cellular Extracts Using TO1-Desthiobiotin Fluorophore Ligand.

Bio Protoc 2018 Apr 5;8(7):e2799. Epub 2018 Apr 5.

Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada.

A native purification strategy using RNA Mango for RNA based purification of RNA-protein complexes is described. The RNA Mango aptamer is first genetically engineered into the RNA of interest. RNA Mango containing complexes obtained from cleared cellular native extracts are then immobilized onto TO1-Desthiobiotin saturated streptavidin agarose beads. Read More

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Analysis of Metals in Whole Cells, Thylakoids and Photosynthetic Protein Complexes in sp. PCC6803.

Bio Protoc 2018 Jun 20;8(12):e2889. Epub 2018 Jun 20.

Department of Plant and Environmental Sciences and Copenhagen Plant Science Centre (CPSC), Faculty of Science, University of Copenhagen, Denmark.

Metals are essential in many biological processes, including oxygenic photosynthesis. Here we described a method to measure the metal pool in whole cells and thylakoids, including the bioactive pool in intact photosynthetic protein complexes in the model oxygenic cyanobacterium PCC6803. In the first part of the protocol, whole cells and thylakoid membranes are carefully prepared, in which the total metal concentrations are measured by inductively coupled plasma triple-quadrupole mass spectrometry (ICP-QQQ-MS). Read More

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PTD-mediated delivery of α-globin chain into Κ-562 erythroleukemia cells and α-thalassemic (HBH) patients' RBCs ex vivo in the frame of Protein Replacement Therapy.

J Biol Res (Thessalon) 2021 Jul 20;28(1):16. Epub 2021 Jul 20.

Laboratory of Pharmacology, Department of Pharmacognosy - Pharmacology, School of Pharmacy, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124, Thessaloniki, Macedonia, Greece.

Background: α-Thalassemia, a congenital hemoglobinopathy, is characterized by deficiency and/or reduced levels of α-globin chains in serious forms of α-thalassemia (HbH disease/Hb Bart's). This research work deals with a Protein Replacement Therapy approach in order to manage α-thalassemia manifestations, caused by the excess of β-globin chain into HbH RBCs. The main goal was to produce the recombinant human α-globin chain in fusion with TAT, a Protein Transduction Domain, to ex vivo deliver it into HbH patients RBCs, to replace the endogenous missing α-globin chain. Read More

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The Ability of Metabolomics to Discriminate Non-Small-Cell Lung Cancer Subtypes Depends on the Stage of the Disease and the Type of Material Studied.

Cancers (Basel) 2021 Jul 1;13(13). Epub 2021 Jul 1.

Metabolomics Laboratory, Clinical Research Centre, Medical University of Bialystok, M. Skłodowskiej-Curie 24a, 15-276 Bialystok, Poland.

Identification of the NSCLC subtype at an early stage is still quite sophisticated. Metabolomics analysis of tissue and plasma of NSCLC patients may indicate new, and yet unknown, metabolic pathways active in the NSCLC. Our research characterized the metabolomics profile of tissue and plasma of patients with early and advanced NSCLC stage. Read More

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Effect of organosolv extraction on the structure and antioxidant activity of eucalyptus kraft lignin.

Int J Biol Macromol 2021 Jul 16. Epub 2021 Jul 16.

College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, PR China; Guangxi Key Laboratory of Clean Pulp & Papermaking and Pollution Control, Nanning 530004, PR China. Electronic address:

In this study, three lignin fractions F1, F2, and F3 were obtained from eucalyptus kraft lignin (EKL) by solvent extraction with ethyl acetate, acetone, and acetone/water. The antioxidant activity of these lignin fractions were measured and evaluated by using the DPPH radical scavenging activity, ABTS radical cation scavenging activity and ferric reducing antioxidant power analysis. These lignin fractions were also characterized by applying Gel permeation chromatography, Fourier transform infrared, P NMR and 2D HSQC NMR techniques. Read More

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Submerged cultivation, characterization and in vitro antitumor activity of polysaccharides from Schizophyllum radiatum.

Int J Biol Macromol 2021 Jul 16;186:919-932. Epub 2021 Jul 16.

Grupo Biopolimer, Facultad de Ciencias Farmacéuticas y Alimentarias, Universidad de Antioquia UdeA, Calle 70 No. 52 - 21, Medellín 050010, Colombia. Electronic address:

Production of polysaccharides by white-rot-fungi in submerged cultivation has several advantages due to process control. This work deals with the submerged cultivation, extraction and antitumor activity of polysaccharides from a wild strain of Schizophyllum radiatum isolated from a tropical forest of Colombia. The mushroom was cultivated in laboratory conditions, and classified by classical and molecular taxonomy. Read More

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Size-exclusion chromatography selective cleanup of aflatoxins in oilseeds followed by HPLC determination to assess the potential health risk.

Toxicon 2021 Jul 17;200:110-117. Epub 2021 Jul 17.

Environment and Bio-Agriculture Department, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.

Aflatoxins (AFs) are one of the most harmful carcinogenic natural toxins that affect food. Crops containing reasonably high oil content may be affected by Aspergillus species and consequently by AF contamination. In this study, a proposed testing method for AF detection in oilseed was developed, validated, and used for a market survey to assess the probabilistic risk exposure caused by consuming contaminated oilseeds including corn, sunflower seed, and soybean. Read More

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Hypocholesterolemic Effect of Blackcurrant () Extract in Healthy Female Subjects: A Pilot Study.

Molecules 2021 Jul 4;26(13). Epub 2021 Jul 4.

Department of Nursing Sciences, Hirosaki University Graduate School of Health Sciences, 66-1 Hon-cho, Hirosaki 036-8564, Japan.

Blackcurrant extract (BCE) ameliorates dyslipidemia in menopausal model animals and in elderly women at a risk of dyslipidemia. However, it is unknown whether the daily intake of BCE can prevent lipid abnormalities in healthy individuals. Lipids are essential for the body, but they also cause arteriosclerosis. Read More

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Correlation of Secondary Particle Number with the Debye-Hückel Parameter for Thickening Mesoporous Silica Shells Formed on Spherical Cores.

ACS Omega 2021 Jul 30;6(27):17734-17740. Epub 2021 Jun 30.

Department of Chemical Engineering, Tohoku University, 6-6-07 Aoba, Aramaki-aza, Aoba-ku, Sendai, Miyagi 980-8579, Japan.

Mesoporous silica shells were formed on nonporous spherical silica cores during the sol-gel reaction to elucidate the mechanism for the generation of secondary particles that disturb the efficient growth of mesoporous shells on the cores. Sodium bromide (NaBr) was used as a typical electrolyte for the sol-gel reaction to increase the ionic strength of the reactant solution, which effectively suppressed the generation of secondary particles during the reaction wherein a uniform mesoporous shell was formed on the spherical core. The number of secondary particles ( ) generated at an ethanol/water weight ratio of 0. Read More

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A Novel Biochemical Study of Anti-Dermal Fibroblast Replicative Senescence Potential of Oligosaccharides.

Front Pharmacol 2021 30;12:690538. Epub 2021 Jun 30.

Research Center of Traditional Chinese Medicine, the Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, China.

Dermal fibroblast replicative senescence that often occurs in aging skin is characterized by loss of cell proliferative capacity, cell cycle arrest, decreased cell elongation, and decreased synthesis of dermal extracellular matrix (ECM) components. Although is known for its effectiveness in alleviating many age-related degenerative diseases, few studies have evaluated components for efficacy or mechanisms of action in delaying cell replicative senescence. In this study, oligosaccharides (PNO) were isolated using a stepwise purification procedure involving water extraction and alcohol precipitation followed by DEAE Sepharose Fast Flow column chromatography, preparative high performance liquid chromatography, and size-exclusion chromatography. Read More

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A transwell assay method to evaluate Borrelia burgdorferi sensu stricto migratory chemoattraction toward tick saliva proteins.

Ticks Tick Borne Dis 2021 Jul 12;12(5):101782. Epub 2021 Jul 12.

Bacterial Diseases Branch, Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, United States. Electronic address:

We developed a transwell assay to quantify migration of the Lyme disease agent, Borrelia burgdorferi sensu stricto (s.s.), toward Ixodes scapularis salivary gland proteins. Read More

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Aloe vera gel extract: Safety evaluation for acute and chronic oral administration in Sprague-Dawley rats and anticancer activity in breast and lung cancer cells.

J Ethnopharmacol 2021 Jul 16;280:114434. Epub 2021 Jul 16.

Department of R&D, Biotech&Science Company of UP, Ltd., Guangzhou, 510000, China. Electronic address:

Ethnopharmacological Relevance: Aloe vera (L.) Burm. f. Read More

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Dye-Loaded Polymersome-Based Lateral Flow Assay: Rational Design of a COVID-19 Testing Platform by Repurposing SARS-CoV-2 Antibody Cocktail and Antigens Obtained from Positive Human Samples.

ACS Sens 2021 Jul 16. Epub 2021 Jul 16.

Department of Biochemistry, Faculty of Science, Ege University, Bornova, 35100 Izmir, Turkey.

The global pandemic of COVID-19 continues to be an important threat, especially with the fast transmission rate observed after the discovery of novel mutations. In this perspective, prompt diagnosis requires massive economical and human resources to mitigate the disease. The current study proposes a rational design of a colorimetric lateral flow immunoassay (LFA) based on the repurposing of human samples to produce COVID-19-specific antigens and antibodies in combination with a novel dye-loaded polymersome for naked-eye detection. Read More

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Photoreaction of photoactivated adenylate cyclase from cyanobacterium Microcoleus chthonoplastes.

J Photochem Photobiol B 2021 Aug 6;221:112252. Epub 2021 Jul 6.

Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan. Electronic address:

The photochemical reaction of photoactivated adenylate cyclase from cyanobacterium Microcoleus chthonoplastes PCC 7420 (mPAC), which consists of a Per-Arnt-Sim (PAS), a light‑oxygene-voltage (LOV), and an adenylate cyclase (AC) domain, was investigated mainly using the time-resolved transient grating method. An absorption spectral change associated with an adduct formation between its chromophore (flavin mononucleotide) and a cysteine residue was observed with a time constant of 0.66 μs. Read More

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[Development of methods for determining and studying the persistence of 2,4-dimethylhydroxybenzene and 2,6-dimethylhydroxybenzene in biological material].

Sud Med Ekspert 2021 ;64(4):53-59

Kursk State Medical University, Kursk, Russia.

Objective: To study the features of the determination and preservation of 2.4-dimethylhydroxybenzene and 2.6-dimethylhydroxybenzene in biological material. Read More

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Gene Locus Disruption and Differences in Renal Epithelial Extracellular Vesicles.

Front Physiol 2021 28;12:630933. Epub 2021 Jun 28.

Department of Pediatrics, University of Tennessee Health Science Center and Le Bonheur Children's Hospital, Memphis, TN, United States.

In tuberous sclerosis complex (TSC), mutations are associated with more severe disease manifestations than mutations and the role of extracellular vesicles (EVs) in this context is not yet studied. We report a comparative analysis of EVs derived from isogenic renal cells except for or gene status and hypothesized that in spite of having similar physical characteristics, EVs modulate signaling pathways differently, thus leading to TSC heterogenicity. We used mouse inner medullary collecting duct (mIMCD3) cells with the (T1G cells) or (T2J cells) gene disrupted by CRISPR/CAS9. Read More

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Coupling size exclusion chromatography to ultracentrifugation improves detection of exosomal proteins from human plasma by LC-MS.

Pract Lab Med 2021 Aug 23;26:e00241. Epub 2021 Jun 23.

Institute for Clinical Chemistry, Medical Faculty Mannheim of the University of Heidelberg, University Hospital Mannheim, Mannheim, Germany.

Objectives: Exosomes are small lipid bilayer vesicles that are defined by their endocytic origin and size range of 30-140 nm. They are constantly produced by different cell types, by both healthy and abnormal cells, and can be isolated from almost all body fluids.Little information exists in isolating exosomes from plasma due to the complexity of its content and the presence of contaminating plasma proteins. Read More

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Putative hexameric glycosyltransferase functional unit revealed by the crystal structure of MurG.

IUCrJ 2021 Jul 8;8(Pt 4):574-583. Epub 2021 May 8.

College of Pharmacy, Chung-Ang University, Seoul 06974, Republic of Korea.

Lipid II, the main component of the bacterial cell wall, is synthesized by the addition of UDP--acetylglucosamine to the UDP--acetylmuramic acid pentapeptide catalyzed by the glycosyltransferase MurG. Owing to its critical role in cell-wall biosynthesis, MurG is considered to be an attractive target for antibacterial agents. Although the Mur family ligases have been extensively studied, the molecular mechanism of the oligomeric scaffolding assembly of MurG remains unclear. Read More

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Allergenicity assessment and allergen profile analysis of different Chinese wheat cultivars.

World Allergy Organ J 2021 Jul 29;14(7):100559. Epub 2021 Jun 29.

Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, PR China.

Backgrounds: As one of the most important cereals, wheat () can cause severe allergic reactions, such as baker's asthma, allergic rhinitis, and atopic dermatitis. A growing number of people are developing allergies to Chinese wheat; however, only a few wheat cultivars have been screened on allergenicity in China.

Objective: The aim of the present study was to assess the allergenicity of different Chinese wheat cultivars and characterize wheat allergen profiles of patients with allergic rhinitis. Read More

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Metal-Organic Framework-Derived Hollow and Hierarchical Porous Multivariate Metal-Oxide Microspheres for Efficient Phosphoproteomics Analysis.

ACS Appl Mater Interfaces 2021 Jul 14. Epub 2021 Jul 14.

National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, P. R. China.

Pre-enrichment of the biological samples is a crucial step in phosphoproteomics research. At present, metal-oxide affinity chromatography (MOAC) is one of the most recognized enrichment strategy. Therefore, the design and preparation of a MOAC-based affinity material with better enrichment properties will be of great significance for the phosphoproteomics study. Read More

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Identification of type B trichothecenes and zearalenone in Chilean cereals by planar chromatography coupled to mass spectroscopy.

Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2021 Jul 13:1-10. Epub 2021 Jul 13.

Department of Food Science and Technology, Faculty of Pharmacy, University of Concepcion, Concepcion, Chile.

High-performance thin-layer chromatography (HPTLC) and HPTLC coupled with mass spectrometry (MS) methods were described for the simultaneous determination of zearalenone (ZEA); type B trichothecenes (TCT-B); nivalenol (NIV) and deoxynivalenol (DON) along with its acetylated derivatives: 3-acetyldeoxynivalenol (3-ADON) and 15-acetyldeoxynivalenol (15-ADON). The extract samples were cleaned-up with Bond Elut Mycotoxin solid-phase extraction cartridges. Then, separation was performed on HPTLC silica gel 60 F plates using toluene, ethyl acetate and formic acid (1:8:1 v/v/v) as mobile phase. Read More

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Characterization of systemic immunosuppression by IDH mutant glioma small extracellular vesicles.

Neuro Oncol 2021 Jul 13. Epub 2021 Jul 13.

UPMC Hillman Cancer Center, Pittsburgh, PA, USA.

Background: Gliomas are the most common primary brain tumors and are universally fatal. Mutations in the isocitrate dehydrogenase genes (IDH1 and IDH2) define a distinct glioma subtype associated with an immunosuppressive tumor microenvironment. Mechanisms underlying systemic immunosuppression in IDH mutant (mutIDH) gliomas are largely unknown. Read More

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A Case of macro-TSH masquerading as subclinical hypothyroidism.

BMJ Case Rep 2021 Jul 12;14(7). Epub 2021 Jul 12.

Endocrinology Laboratory, Royal Victoria Hospital, Belfast, UK.

A 47-year-old man was commenced on levothyroxine following a diagnosis of subclinical hypothyroidism with nonspecific symptoms. Despite increasing doses of levothyroxine, his thyroid-stimulating hormone (TSH) remained elevated and he was referred for further assessment as he was unable to tolerate further titration. On assessment, his thyroid function demonstrated an elevated TSH and elevated free-T4. Read More

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A trivalent TNF-R2 as a new tumor necrosis factor alpha-blocking molecule.

Proteins 2021 Jul 11. Epub 2021 Jul 11.

Recombinant Biopharmaceuticals Laboratory, Pharmacology Department, School of Biological Sciences, Universidad de Concepción, Concepción, Chile.

The neutralization of tumor necrosis factor alpha (TNFα) with biopharmaceuticals is a successful therapy for inflammatory diseases. Currently, one of the main TNFα-antagonists is Etanercept, a dimeric TNF-R2 ectodomain. Considering that TNFα and its receptors are homotrimers, we proposed that a trimeric TNF-R2 ectodomain could be an innovative TNFα-antagonist. Read More

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Glycoconjugation of Shigella flexneri type 2a O-polysaccharide with CRM as a potential vaccine candidate for shigellosis.

Biologicals 2021 Jul 8. Epub 2021 Jul 8.

Department of Biological Sciences, BITS Pilani Hyderabad Campus, Jawahar Nagar, Hyderabad Campus, Kapra Mandal, Medchal, Telangana, 500078, India. Electronic address:

Shigellosis, a diarrheal disorder caused by an entero-invasive bacterium Shigella, is a major concern among children often leading to mortality. As most of these strains have developed universal antibiotic resistance, the development of a vaccine is crucial in combating the infection. The O-specific polysaccharide (O-PSs) from S. Read More

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An Aspergillus nidulans endo-β-1,3-glucanase exhibited specific catalytic features and was used to prepare 3-O-β-cellobiosyl-d-glucose and 3-O-β-gentiobiosyl-d-glucose with high antioxidant activity from barley β-glucan and laminarin, respectively.

Int J Biol Macromol 2021 Jul 9;186:424-432. Epub 2021 Jul 9.

Jiangsu Key Laboratory for Microbes and Microbial Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Science, Nanjing Normal University, 1 Wenyuan Road, Nanjing, Jiangsu 210023, PR China. Electronic address:

An endo-β-1,3(4)-glucanase AnENG16A from Aspergillus nidulans shows distinctive catalytic features for hydrolysis of β-glucans. AnENG16A hydrolyzed Eisenia bicyclis laminarin to mainly generate 3-O-β-gentiobiosyl-d-glucose and hydrolyzed barley β-glucan to mainly produce 3-O-β-cellobiosyl-d-glucose. Using molecular exclusion chromatography, we isolated and purified 3-O-β-cellobiosyl-d-glucose and 3-O-β-gentiobiosyl-d-glucose, respectively, from AnENG16A-hydrolysate of barley β-glucan and E. Read More

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