6,313 results match your criteria excised rat

Multimodal imaging of drug and excipients in rat lungs following an inhaled administration of controlled-release drug laden PLGA microparticles.

Analyst 2021 Apr 20. Epub 2021 Apr 20.

GlaxoSmithKline, Gunnels Wood Road, Stevenage, SG1 2NY, UK.

Controlled-release formulations, in the form of micro- or nanoparticles, are increasingly attractive to the pharmaceutical industry for drug delivery. For respiratory illnesses, controlled-release microparticle formulations provide an opportunity to deliver a higher percentage of an inhaled medicament dose to the lung, thus potentially reducing the therapeutic dose, frequency of dosing, and minimising side-effects. We describe the use of a multimodal approach consisting of MALDI MS imaging, 3D depth profiling TOF-SIMS analysis, and histopathology to monitor the distribution of drug and excipients in sections taken from excised rat lungs following an inhaled administration of drug-laden microparticles. Read More

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Autofluorescence spectroscopy and multivariate analysis for predicting the induced damages to other organs due to liver fibrosis.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Mar 24;257:119741. Epub 2021 Mar 24.

Division of Biophotonics and Imaging, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India. Electronic address:

When our liver does not work well, it can induce damage to other organs causing their dysfunction. With this background, we aim to study the effect of liver fibrosis on other organs such as heart, lungs, kidney and spleen by assessing the variations in the inherent emission property of the tissue, using fluorescence spectroscopy. Fluorescence emission spectra from excised organs of liver fibrosis induced rats were collected at excitation wavelengths 320 and 410 nm. Read More

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Nipple Engineering: Maintaining Nipple Geometry with Externally Scaffolded Processed Autologous Costal Cartilage.

J Plast Reconstr Aesthet Surg 2021 Mar 24. Epub 2021 Mar 24.

Laboratory of Bioregenerative Medicine & Surgery, Division of Plastic Surgery, Weill Cornell Medicine, New York, NY, United States of America; Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, United States of America. Electronic address:

Introduction: Nipple reconstruction is the essential last step of breast reconstruction after total mastectomy, resulting in improved general and aesthetic satisfaction. However, most techniques are limited by secondary scar contracture and loss of neo-nipple projection leading to patient dissatisfaction. Approximately, 16,000 patients undergo autologous flap breast reconstruction annually, during which the excised costal cartilage (CC) is discarded. Read More

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Effects Of Intracerebroventricularly Administered Opioid Peptide Antagonists On Tissue Glycogen Levels In Rats After Exercise.

Turk J Med Sci 2021 Apr 17. Epub 2021 Apr 17.

Background/aim: Physical exercise is a state of physiological stress that requires adaptation of the organism to physical activity. Glycogen is an important and essential energy source for muscle contraction. Skeletal muscle and liver are two important glycogen stores and the energy required to maintain exercise in rodents are provided by destruction of this glycogen depot. Read More

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Effects of soy-containing infant formulas on thyroid gland in rats.

Minerva Pediatr (Torino) 2021 Apr 16. Epub 2021 Apr 16.

Department of Histology and Embriology, Faculty of Medicine, Yeditepe University, Istanbul Turkey.

Background: Effects of soy-containing infant formulas on the thyroid gland is not clear. We aimed to evaluate the effects of infant formulas with different quantities of soy content on the functional and histopathological characteristics of the thyroid gland.

Methods: Twenty-eight female Sprague-Dawley rats were divided into four groups. Read More

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Potassium bromate (KBrO3) Modulates Oxidative stress and inflammatory biomarkers in NaOH induced crohn's colitis in Wistar rats.

Can J Physiol Pharmacol 2021 Apr 13. Epub 2021 Apr 13.

University of Ibadan, Faculty of Basic Medical Sciences, Physiology, Ibadan, Oyo, Nigeria.

Potassium bromate (KBrO3) present in consumed ozonised water was recently documented to exacerbate experimental gastric ulcer. Report is however vague as regards its effects in the colon; where water reabsorption occurs. In this study, we observed the possible effects of KBrO3 on oxidative stress and inflammatory biomarkers in NaOH induced crohn's colitis. Read More

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[Effects of normobaric hyperoxia intervention on renal ischemia-reperfusion injury in rats].

Zhonghua Yi Xue Za Zhi 2021 Apr;101(14):1036-1040

Department of Nephrology, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.

To investigate the effects of normobaric hyperoxia intervention on renal ischemia-reperfusion injury in rats and its possible mechanism. Twenty-one adult male SD rats were enrolled and their right kidneys were excised. After two weeks, they were randomly assigned to 3 groups, with 7 rats in each group, namely sham-operated group (Group S), ischemia-reperfusion group (Group I/R), and normobaric hyperoxia+ischemia-reperfusion group (Group NBHO+I/R). Read More

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Untypical Metabolic Adaptations in Spontaneously Hypertensive Rats to Free Running Wheel Activity Includes Uncoupling Protein-3 (UCP-3) and Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Expression.

Front Physiol 2021 23;12:598723. Epub 2021 Mar 23.

Department of Medicine, Institute of Physiology, Justus Liebig University Giessen, Giessen, Germany.

Obesity and hypertension are common risk factors for cardiovascular disease whereas an active lifestyle is considered as protective. However, the interaction between high physical activity and hypertension is less clear. Therefore, this study investigates the impact of high physical activity on the muscular and hepatic expression of glucose transporters (Glut), uncoupling proteins (UCPs), and proprotein convertase subtilisin/kexin type 9 (PCSK9) in spontaneously hypertensive rats (SHRs). Read More

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Hypolipidemic and anti-atherosclerogenic effects of aqueous extract of Ipomoea batatas leaves in diet-induced hypercholesterolemic rats.

J Integr Med 2021 Mar 2. Epub 2021 Mar 2.

Department of Animal Biology and Physiology, University of Yaoundé 1, Yaoundé, Centre Region, Cameroon.

Objective: Ipomoea batatas (L.) Lam. is a food plant used in African traditional medicine to treat cardiovascular diseases and related conditions. Read More

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Sodium-glucose cotransporter 2 inhibition prevents renal fibrosis in cyclosporine nephropathy.

Acta Diabetol 2021 Mar 24. Epub 2021 Mar 24.

Dipartimento Di Scienze Radiologiche, Oncologiche E Anatomopatologiche, Istituto Di Anatomia Patologica Sapienza Universita' Di Roma, Roma, Italy.

Aims: Sodium-glucose cotransporter 2 (SGLT2) inhibitors, a new class of antidiabetic drugs, are nephroprotective in case of diabetes, but whether a similar beneficial effect may be detectable also in case of chronic non-diabetic kidney diseases remains still unknown. The aim of this study was to evaluate the effects of empagliflozin, a SGLT-2 inhibitor, on the progression of cyclosporine nephropathy, in the absence of diabetes.

Methods: Sprague Dawley rats (n = 27) have been fed with low-salt diet starting 10 days before the beginning and finished at the end of the experimental period. Read More

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Aqueous extract of prevents Cadmium Chloride-induced testicular damage in Wistar Rats.

J Basic Clin Physiol Pharmacol 2021 Mar 11. Epub 2021 Mar 11.

School of Medicine and Allied Health Sciences, University of the Gambia, Banjul, The Gambia.

Objectives: The role of aqueous extract of was investigated against cadmium chloride-induced testicular damage in Wistar Rats.

Methods: Thirty (30) male Wistar Rats weighing (150-170) were divided into six groups (n=5). Group A served as control and received oral administration of phosphate buffer saline; group B received 800 mg/kg only; group C were injected intraperitoneally with single dose 2 mg/kg b. Read More

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Loss of muscular force in isolated rat diaphragms is related to changes in muscle fibre size.

Physiol Meas 2021 Mar 11;42(2):025003. Epub 2021 Mar 11.

Department of Anesthesiology and Critical Care, Medical Center-University of Freiburg, Faculty of Medicine, University of Freiburg, Germany.

Objective: Passivity of the diaphragm during prolonged mechanical ventilation can lead to ventilation-induced diaphragmatic dysfunction reasoned by a reduction of diaphragmatic muscle strength. Electrical stimulation may be utilised to modulate diaphragm muscle strength. Therefore we intended to investigate diaphragmatic muscle strength based on stimulation with electric impulses. Read More

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Neurotensin: A Neuropeptide induced by hCG in the Human and Rat Ovary during the Periovulatory Period.

Biol Reprod 2021 Mar 4. Epub 2021 Mar 4.

Department of Obstetrics and Gynecology, College of Medicine, University of Kentucky, 800 Rose Street, Lexington, Kentucky, USA 40536-0298.

Neurotensin (NTS) is a tridecapeptide that was first characterized as a neurotransmitter in neuronal cells. The present study examined ovarian NTS expression across the periovulatory period in the human and the rat. Women were recruited into this study and monitored by transvaginal ultrasound. Read More

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Development and Characterization of Niosomal Gel System using mucilage for the treatment of Rheumatoid Arthritis.

Iran J Pharm Res 2020 ;19(3):465-482

Department of Pharmaceutical Science, Shalom Institute of Health and Allied Sciences, SHUATS, Prayagraj (211007), Uttar Pradesh, India.

Niosomes structural framework comprises of non-ionic surfactant-based microscopic lamellar structures which carries the potential to sustain the effect of drug from its delivery system. In present work, the attempt was made to identify the effect of different ingredients such as effect of Tweens and natural mucilage of on the performance of developed niosomal gel formulations in order to prolong the duration of action of drug and to minimize its side effects of topical conventional drug administration. All Ibuprofen loaded niosomes formulationswere prepared by ether injection method; using cetosteryl alcohol with different variants of Tweens and Spans. Read More

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January 2020

Propoxur (PPx) exposure as hormonal disruptor and spermatocidal in rat model.

Morphologie 2021 Mar 4. Epub 2021 Mar 4.

Department of Anatomy, University of Ilorin, Ilorin, Nigeria; Department of Anatomy, Osun State University, Osogbo, Nigeria; Department of Anatomical Sciences, University of Abuja, Nigeria.

Background: Propoxur is a carbamate insecticide widely used both in indoor and outdoor place to control insects. This present work was conducted to study the effect of exposure of propoxur (PPX) on hormonal and histological changes in the rat testes.

Methods: The control animals received distil water, while the treated animals received Propoxur (PPx) by inhalation every other day for one month (PPx-1) and two months (PPx-2) respectively. Read More

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Reconstruction of functional uterine tissues through recellularizing the decellularized rat uterine scaffolds by MSCs in vivo and in vitro.

Biomed Mater 2021 Mar 3;16(3):035023. Epub 2021 Mar 3.

Savaid Medical School, University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.

Infertile people who suffered from loss of uterine structures and/or functions can be treated through gestational surrogacy or uterus transplantation, which remains challenging due to the ethical and social issues, the lack of donor organs as well as technical and safety risks. One promising solution is to regenerate and reconstruct a bioartificial uterus for transplantation through the engineering of uterine architecture and appropriate cellular constituents. Here, we developed a well-defined system to regenerate a functional rat uterine through recellularization of the decellularized uterine matrix (DUM) patches reseeded with human mesenchymal stem cells (hMSCs). Read More

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Phase retrieval-based phase-contrast CT for vascular imaging with microbubble contrast agent.

Med Phys 2021 Mar 3. Epub 2021 Mar 3.

Department of Radiology, Rui Jin Hospital, Shanghai Jiao Tong University, and School of Medicine, Shanghai, China.

Purpose: The introduction of microbubble contrast agent into tissues can create significant phase shifts. Phase retrieval (PR)-based phase-contrast CT (PCCT) is an imaging method for retrieving and reconstructing the phase shifts within an object. This study aimed to evaluate the feasibility of PR-based PCCT with microbubble contrast agent for vascular imaging. Read More

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Anti-Inflammatory Activity of Human Lens Crystallin Derived Peptide.

Curr Drug Deliv 2021 Mar 2. Epub 2021 Mar 2.

Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal - 576104, Karnataka. India.

Background: Inflammation has become the culmination point for several chronic diseases like skin diseases, asthma, neurological disorders, cancer and cardiovascular disorders. Mini αA-crystallin peptide, identified from a highly conserved region of human lens protein αA-crystallin, is known to have a chaperone-like function, hence has generated interest in exploring the anti-inflammatory potential of the peptide.

Objective: The objective of the study was to evaluate anti-inflammatory potential of mini αA chaperone using in vitro, ex-vivo and in vivo models. Read More

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The Effect of Apocynin on Fat Graft Survival.

Aesthetic Plast Surg 2021 Mar 1. Epub 2021 Mar 1.

ENT Department, Taksim Training and Education Hospital, Istanbul, Turkey.

Background: In this study, we investigated the effect of apocynin on fat graft survival in a rat model.

Methods: Twenty-one Wistar albino male rats were included in the study. The grafts taken from the inguinal area were applied to the nape of each rat. Read More

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A freeze-and-thaw-induced fragment of the microtubule-associated protein tau in rat brain extracts: implications for the biochemical assessment of neurotoxicity.

Biosci Rep 2021 Mar;41(3)

Department of Biochemistry and Immunology, Ribeirao Preto Medical School, University of São Paulo, SP 14049-900, Brazil.

Tau is a microtubule-associated protein (MAP) responsible for controlling the stabilization of microtubules in neurons. Tau function is regulated by phosphorylation. However, in some neurological diseases Tau becomes aberrantly hyperphosphorylated, which contributes to the pathogenesis of neurological diseases, known as tauopathies. Read More

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Pre-exposure of voglibose exerts cerebroprotective effects through attenuating activation of the polyol pathway and inflammation.

Eur J Neurosci 2021 Feb 20. Epub 2021 Feb 20.

Department of Pharmacology, Institute of Pharmacy, Nirma University, Ahmadabad, Gujarat, India.

Chronic hyperglycemia induces activation of the polyol-sorbitol pathway, which is a major contributor to microvascular complications like stroke. The current study was designed to elucidate the therapeutic role of α-glucose inhibitor in chronic hyperglycemia-induced impaired polyol pathway and associated micro-complications. Male albino-Wistar rats (200-250 g) were treated with voglibose 10 mg kg  day /p. Read More

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February 2021

The effect of autologous repair and voluntary wheel running on force recovery in a rat model of volumetric muscle loss.

Exp Physiol 2021 Apr 2;106(4):994-1004. Epub 2021 Mar 2.

Regenerative Biomaterials Lab, Department of Biomedical Engineering University of Arkansas, Fayetteville, AR, USA.

New Findings: What is the central question of this study? Following large traumatic loss of muscle tissue (volumetric muscle loss; VML), permanent functional and cosmetic deficits present themselves and regenerative therapies alone have not been able to generate a robust regenerative response: how does the addition of rehabilitative therapies affects the regenerative response? What is the main finding and its importance? Using exercise along with autologous muscle repair, we demonstrated accelerated muscle force recovery response post-VML. The accentuated force recovery 2 weeks post-VML would allow patients to return home sooner than allowed with current therapies.

Abstract: Skeletal muscle can regenerate from damage but is overwhelmed with extreme tissue loss, known as volumetric muscle loss (VML). Read More

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Polymeric Precipitation Inhibitor Based Supersaturable Self-microemulsifying Drug Delivery System of Canagliflozin: Optimization and Evaluation.

Curr Drug Deliv 2021 02 17. Epub 2021 Feb 17.

Department of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar, Punjab. India.

Background: The present investigation attempts to optimize Supersaturable lipid based formulation (SS SMEDDS) of Biopharmaceutical Classification System (BCS) class IV drug canagliflozin (CFZ) and evaluating the oral bioavailability of the formulation.

Methods: Preliminary screening revealed Poloxamer 188 to most effectively inhibit precipitation of CFZ after dispersion during in vitro supersaturation studies. Box Behnken Design was employed for designing different formulations and various statistical analysis were done out to select an appropriate mathematical model. Read More

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February 2021

Orthograde signal of dihydropyridine receptor increases Ca leakage after repeated contractions in rat fast-twitch muscles in vivo.

Am J Physiol Cell Physiol 2021 Feb 17. Epub 2021 Feb 17.

Graduate School of Humanities and Social Sciences, Hiroshima University, Hiroshima, Japan.

The purpose of this study was to investigate the mechanism underlying sarcoplasmic reticulum (SR) Ca leakage after in vivo contractions. Rat gastrocnemius muscles were electrically stimulated in vivo, and then mechanically skinned fibers and SR microsomes were prepared from the muscles excised 30 min after repeated high-intensity contractions. The mechanically skinned fibers maintained the interaction between dihydropyridine receptors (DHPRs) and ryanodine receptors (RyRs), whereas the SR microsomes did not. Read More

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February 2021

Promotes Healing of Excised Wounds in Rat Skin: Emphasis on Its Collagen-Enhancing, Antioxidant, and Anti-Inflammatory Activities.

Oxid Med Cell Longev 2021 26;2021:8891445. Epub 2021 Jan 26.

Department of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia.

Steud () belongs to the family Asteraceae. It has several uses in folk medicine in the Arabian Peninsula. The current study aimed at evaluating the wound healing properties of the extract in rats. Read More

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January 2021

Efficacy of shear wave elastography for evaluating right ventricular myocardial fibrosis in monocrotaline-induced pulmonary hypertension rats.

J Cardiol 2021 Feb 7. Epub 2021 Feb 7.

Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan.

Background: Right ventricular (RV) function is important for outcomes in pulmonary hypertension. Evaluation of RV myocardial characteristics is useful to assess the disease severity. Shear wave elastography (SWE) provides information of shear wave (SW) elasticity, which is related to tissue hardness, and SW dispersion slope, which reflects tissue viscosity. Read More

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February 2021

Interference with TGFβ1-Mediated Inflammation and Fibrosis Underlies Reno-Protective Effects of the CB1 Receptor Neutral Antagonists AM6545 and AM4113 in a Rat Model of Metabolic Syndrome.

Molecules 2021 Feb 6;26(4). Epub 2021 Feb 6.

Center for Drug Discovery, Northeastern University, Boston, MA 02115, USA.

The role of cannabinoid receptors in nephropathy is gaining much attention. This study investigated the effects of two neutral CB1 receptor antagonists, AM6545 and AM4113, on nephropathy associated with metabolic syndrome (MetS). MetS was induced in rats by high-fructose high-salt feeding for 12 weeks. Read More

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February 2021

Graviola mitigates acetic acid-induced ulcerative colitis in rats: insight on apoptosis and Wnt/Hh signaling crosstalk.

Environ Sci Pollut Res Int 2021 Feb 9. Epub 2021 Feb 9.

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.

In this study, we elucidated the potential protective effects of graviola leaves, compared with sulfasalazine, against acetic acid (AA)-induced ulcerative colitis (UC) in rats. Twenty-eight mature male rats were divided into four groups, Sham, Colitis, Colitis/Sulfa, and Colitis/Graviola, and were treated orally with either saline, saline, sulfasalazine (100 mg/kg/day), or graviola (100 mg/kg/day), respectively, for 7 days. On the 4th day, UC was induced by transrectal administration of 4% AA. Read More

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February 2021

1,25-(OH)D ameliorates renal interstitial fibrosis in UUO rats through the AMPKα/mTOR pathway.

J Int Med Res 2021 Feb;49(2):300060520981360

Department of Nephrology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, China.

Objective: To investigate the effects of 1,25(OH)D on renal fibrosis associated with the AMP-activated protein kinase (AMPK)α/mechanistic target of rapamycin (mTOR) signalling pathway in a rat model of unilateral ureteral obstruction (UUO).

Methods: A total of 54 male Sprague Dawley rats were randomly divided into three groups: sham-operation group, UUO group, and UUO plus calcitriol (3 ng/100 g) group. Renal tissue was excised for histological examination by immunohistochemistry and Western blot, and for gene expression analysis using real-time polymerase chain reaction. Read More

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February 2021

Histomorphological testicular changes and decrease in the sperm count in pubertal rats induced by a high-sugar diet.

Ann Anat 2021 May 28;235:151678. Epub 2021 Jan 28.

Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México. Electronic address:

Background: During childhood and adolescence, excessive food consumption stimulates adipose tissue expansion promoting overweight in humans, and mice. A high-sucrose diet is related to obesity and metabolic syndrome. Infertility is commonly related to these pathologies. Read More

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