470 results match your criteria evs range


Comparative analysis of non-exhaust airborne particles from electric and internal combustion engine vehicles.

J Hazard Mater 2021 Jul 10;420:126626. Epub 2021 Jul 10.

Brembo S.p.A., Via Europa 2, 24020 Stezzano (BG), Italy.

This paper evaluates the effect of the electrification of the small, medium, and large internal combustion engine (ICE) passenger cars on the levels of total particulate matter (PM). The total mean PM and PM emission factors (EFs) on urban, rural, and motorway roads are in the range of 26.13 - 39. Read More

View Article and Full-Text PDF

Coupling size exclusion chromatography to ultracentrifugation improves detection of exosomal proteins from human plasma by LC-MS.

Pract Lab Med 2021 Aug 23;26:e00241. Epub 2021 Jun 23.

Institute for Clinical Chemistry, Medical Faculty Mannheim of the University of Heidelberg, University Hospital Mannheim, Mannheim, Germany.

Objectives: Exosomes are small lipid bilayer vesicles that are defined by their endocytic origin and size range of 30-140 nm. They are constantly produced by different cell types, by both healthy and abnormal cells, and can be isolated from almost all body fluids.Little information exists in isolating exosomes from plasma due to the complexity of its content and the presence of contaminating plasma proteins. Read More

View Article and Full-Text PDF

Orchestrating Extracellular Vesicle With Dual Reporters for Imaging and Capturing in Mammalian Cell Culture.

Front Mol Biosci 2021 18;8:680580. Epub 2021 Jun 18.

Department of Bioengineering, School of Engineering, Santa Clara University, Santa Clara, CA, United States.

Recent technological advancements have enabled live-cell imaging of intracellular organelles to monitor their biogenesis in mammalian cells. However, applying this method to gain insight into extracellular organelles, such as extracellular vesicles (EVs), presents unique challenges that require special considerations in design and engineering. We have developed a dual-reporter system that combines genetic fusion, fluorescence microcopy and magnetic beads capture of EVs to study the biogenesis of EVs in mammalian cell cultures. Read More

View Article and Full-Text PDF

Identification of extracellular nanoparticle subsets by nuclear magnetic resonance.

Chem Sci 2021 Apr 29;12(24):8311-8319. Epub 2021 Apr 29.

NMR Research Unit, University of Oulu Finland

Exosomes are a subset of secreted lipid envelope-encapsulated extracellular vesicles (EVs) of 50-150 nm diameter that can transfer cargo from donor to acceptor cells. In the current purification protocols of exosomes, many smaller and larger nanoparticles such as lipoproteins, exomers and microvesicles are typically co-isolated as well. Particle size distribution is one important characteristics of EV samples, as it reflects the cellular origin of EVs and the purity of the isolation. Read More

View Article and Full-Text PDF

Impact of various air mass types on cloud condensation nuclei concentrations along coastal southeast Florida.

Atmos Environ (1994) 2021 Jun 29;254. Epub 2021 Mar 29.

Department of Chemical and Environmental Engineering, University of Arizona, Tucson, AZ, USA.

Coastal southeast Florida experiences a wide range of aerosol conditions, including African dust, biomass burning (BB) aerosols, as well as sea salt and other locally-emitted aerosols. These aerosols are important sources of cloud condensation nuclei (CCN), which play an essential role in governing cloud radiative properties. As marine environments dominate the surface of Earth, CCN characteristics in coastal southeast Florida have broad implications for other regions with the added feature that this site is perturbed by both natural and anthropogenic emissions. Read More

View Article and Full-Text PDF

Extracellular Vesicles and Their miRNA Content in Amniotic and Tracheal Fluids of Fetuses with Severe Congenital Diaphragmatic Hernia Undergoing Fetal Intervention.

Cells 2021 Jun 14;10(6). Epub 2021 Jun 14.

Fetal Medicine and Surgery Service, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, 20122 Milan, Italy.

Infants with congenital diaphragmatic hernia (CDH) are at high risk of postnatal mortality due to lung hypoplasia and arterial pulmonary hypertension. In severe cases, prenatal intervention by fetal endoscopic tracheal occlusion (FETO) can improve survival by accelerating lung growth. However, postnatal mortality remains in the range of about 50% despite fetal treatment, and there is currently no clear explanation for this different clinical response to FETO. Read More

View Article and Full-Text PDF

Implantable and transcutaneous continuous glucose monitoring system: a randomized cross over trial comparing accuracy, efficacy and acceptance.

J Endocrinol Invest 2021 Jul 1. Epub 2021 Jul 1.

Department of Medicine, University of Padova, Via Giustiniani 2, 35128, Padova, Italy.

Aim: To compare accuracy, efficacy and acceptance of implantable and transcutaneous continuous glucose monitoring (CGM) systems.

Methods: In a randomized crossover trial we compared 12 weeks with Eversense implantable sensor (EVS) and 12 weeks with Dexcom G5 transcutaneous sensor (DG5) in terms of accuracy, evaluated as Mean Absolute Relative Difference (MARD) vs capillary glucose (SMBG), time of CGM use, adverse events, efficacy (as HbA1c, time in range, time above and below range) and psychological outcomes evaluated with Diabetes Treatment Satisfaction Questionnaire (DTSQ), Glucose Monitoring Satisfaction Survey (GMSS), Hypoglycemia Fear Survey (HFS2), Diabetes Distress Scale (DDS).

Results: 16 subjects (13 males, 48. Read More

View Article and Full-Text PDF

Extracellular Vesicles-The Next Frontier in Endocrinology.

Endocrinology 2021 Sep;162(9)

Department of Molecular and Integrative Physiology, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA.

Extracellular vesicles (EVs), including exosomes, are emerging as important carriers of signals in normal and pathological physiology. As EVs are a long-range communication or signaling modality-just like hormones are-the field of endocrinology is uniquely poised to offer insight into their functional biology and regulation. EVs are membrane-bound particles secreted by many different cell types and can have local or systemic effects, being transported in body fluids. Read More

View Article and Full-Text PDF
September 2021

Exosomes and Melatonin: Where Their Destinies Intersect.

Front Immunol 2021 11;12:692022. Epub 2021 Jun 11.

Department of Cell Systems and Anatomy, University of Texas (UT) Health, San Antonio, TX, United States.

Cell-to-cell communication is a broad and complex process associated with regular stimuli to maintain healthy cell interactions. One of the agents capable of cellular communication is known as an exosome, a subset of extracellular vesicles (EVs) released by the cell membrane. The exosome contains a wide range of functional proteins, mRNAs and miRNAs, which have the potential to interact with healthy or diseased cells in the body. Read More

View Article and Full-Text PDF

Isolation of exosomes from serum of patients with lung cancer: a comparison of the ultra-high speed centrifugation and precipitation methods.

Ann Transl Med 2021 May;9(10):882

Department of Instrument Science & Engineering, School of Electronic Information & Electrical Engineering, Shanghai Engineering Research Center for Intelligent Diagnosis & Treatment Instrument, Institute of Nano Biomedicine & Engineering, Shanghai Jiaotong University, Shanghai, China.

Background: Extracellular vesicles (EVs) is a group of heterogeneous cell-derived membrane structures, which is composed of a large number of exosomes released by cells, microbubbles (MVs) and apoptotic bodies. The formation of exocrine body is a process of fine regulation, which includes four stages: initiation, endocytosis, polycystic body formation and exocrine body secretion. Ultracentrifugation is currently the gold standard for external body separation; it includes a series of centrifugation steps at a rotation speed of 100,000 rpm or more to purify exocrine bodies from protein contaminants. Read More

View Article and Full-Text PDF

New Multiscale Characterization Methodology for Effective Determination of Isolation-Structure-Function Relationship of Extracellular Vesicles.

Front Bioeng Biotechnol 2021 7;9:669537. Epub 2021 Jun 7.

Sydney School of Pharmacy, Faculty of Medicine and Health, Sydney Nano Institute, The University of Sydney, Camperdown, NSW, Australia.

Extracellular vesicles (EVs) have been lauded as next-generation medicines, but very few EV-based therapeutics have progressed to clinical use. Limited clinical translation is largely due to technical barriers that hamper our ability to mass produce EVs, i.e. Read More

View Article and Full-Text PDF

Enterovirus D68 in hospitalized children with respiratory symptoms in Guangdong from 2014 to 2018: Molecular epidemiology and clinical characteristics.

J Clin Virol 2021 Jun 4;141:104880. Epub 2021 Jun 4.

Innovation Platform for Public Health of Guangdong Province, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, China; Department of Medical Laboratory, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong 510515, China. Electronic address:

Background: Enterovirus D68 (EV-D68) is an emerging pathogen in humans. EV-D68 causes a wide range of respiratory symptoms in children and has the propensity to cause severe complications. EV-D68 outbreaks are rarely investigated in mainland China. Read More

View Article and Full-Text PDF

Therapeutic uses of post-partum tissue-derived mesenchymal stromal cell secretome.

Indian J Med Res 2020 12;152(6):541-552

Department of Zoology & Environment Sciences, Faculty of Science, University of Colombo, Colombo, Sri Lanka.

Human post-partum tissue mesenchymal stromal cells (hPPT-MSCs) are widely used in research to investigate their differentiation capabilities and therapeutic effects as potential agents in cell-based therapy. This is ascribed to the advantages offered by the use of MSCs isolated from hPPT over other MSC sources. A paradigm shift in related research is evident that focuses on the secretome of the human MSCs (hMSCs), as therapeutic effects of hMSCs are attributed more so to their secreted growth factors, cytokines and chemokines and to the extracellular vesicles (EVs), all of which are components of the hMSC secretome. Read More

View Article and Full-Text PDF
December 2020

Ultrawideband (UWB)-based precise short-range localization for wireless power transfer to electric vehicles in parking environments.

Authors:
Seung-Mok Lee

PeerJ Comput Sci 2021 27;7:e567. Epub 2021 May 27.

Department of Automotive System Engineering, Keimyung University, Daegu, South Korea.

As the necessity of wireless charging to support the popularization of electric vehicles (EVs) emerges, the development of a wireless power transfer (WPT) system for EV wireless charging is rapidly progressing. The WPT system requires alignment between the transmitter coils installed on the parking lot floor and the receiver coils in the vehicle. To automatically align the two sets of coils, the WPT system needs a localization technology that can precisely estimate the vehicle's pose in real time. Read More

View Article and Full-Text PDF

Cancer therapy-related cardiac dysfunction: is endothelial dysfunction at the heart of the matter?

Clin Sci (Lond) 2021 Jun;135(12):1487-1503

Toronto General Hospital Research Institute, University Health Network, Toronto, Canada.

Significant improvements in cancer survival have brought to light unintended long-term adverse cardiovascular effects associated with cancer treatment. Although capable of manifesting a broad range of cardiovascular complications, cancer therapy-related cardiac dysfunction (CTRCD) remains particularly common among the mainstay anthracycline-based and human epidermal growth factor receptor-targeted therapies. Unfortunately, the early asymptomatic stages of CTRCD are difficult to detect by cardiac imaging alone, and the initiating mechanisms remain incompletely understood. Read More

View Article and Full-Text PDF

Routing of Electric Vehicles With Intermediary Charging Stations: A Reinforcement Learning Approach.

Front Big Data 2021 26;4:586481. Epub 2021 May 26.

Photovoltaics and Thin Film Electronics Laboratory (PV-Lab), Institute of Microengineering (IMT), École Polytechnique Fédérale de Lausanne (EPFL), Neuchâtel, Switzerland.

In the past few years, the importance of electric mobility has increased in response to growing concerns about climate change. However, limited cruising range and sparse charging infrastructure could restrain a massive deployment of electric vehicles (EVs). To mitigate the problem, the need for optimal route planning algorithms emerged. Read More

View Article and Full-Text PDF

Proximity Proteomics Has Potential for Extracellular Vesicle Identification.

J Proteome Res 2021 Jul 11;20(7):3519-3531. Epub 2021 Jun 11.

Department of Biochemistry, Saitama Medical University, 38 Morohongo, Moroyama-machi, Iruma-gun, Saitama 350-0495, Japan.

Extracellular vesicles (EVs) are biomarkers and mediators of intercellular communication. In biological samples, EVs are secreted by various types of cells. The proteomic identification of proteins expressed in EVs has potential to contribute to research and clinical applications, particularly for cancer. Read More

View Article and Full-Text PDF

Sunitinib and Axitinib increase secretion and glycolytic activity of small extracellular vesicles in renal cell carcinoma.

Cancer Gene Ther 2021 Jun 4. Epub 2021 Jun 4.

Division of Hematology and Oncology, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA.

Extracellular vesicles (EVs) encompass a wide range of vesicles that are released by all cell types. They package protein, nucleic acids, metabolites, and other cargo that can be delivered to recipient cells and affect their phenotypes. However, little is known about how pharmaceutical agents can alter EV secretion, protein and metabolic cargo, and the active biological processes taking place in these vesicles. Read More

View Article and Full-Text PDF

Extracellular vesicle-based therapy for amyotrophic lateral sclerosis.

Brain Circ 2021 Jan-Mar;7(1):23-28. Epub 2021 Mar 30.

Department of Neurosurgery and Brain Repair, Morsani College of Medicine, University of South Florida, Tampa, FL, USA.

Amyotrophic lateral sclerosis (ALS) stands as a neurodegenerative disorder characterized by the rapid progression of motor neuron loss in the brain and spinal cord. Unfortunately, treatment options for ALS are limited, and therefore, novel therapies that prevent further motor neuron degeneration are of dire need. In ALS, the infiltration of pathological elements from the blood to the central nervous system (CNS) compartment that spur motor neuron damage may be prevented via restoration of the impaired blood-CNS-barrier. Read More

View Article and Full-Text PDF

Regulatory and Technical Constraints: An Overview of the Technical Possibilities and Regulatory Limitations of Vehicle Telematic Data.

Sensors (Basel) 2021 May 18;21(10). Epub 2021 May 18.

Kemmy Business School (KBS), University of Limerick, V94 T9PX Limerick, Ireland.

A telematics device is a vehicle instrument that comes preinstalled by the vehicle manufacturer or can be added later. The device records information about driving behavior, including speed, acceleration, and turning force. When connected to vehicle computers, the device can also provide additional information regarding the mechanical usage and condition of the vehicle. Read More

View Article and Full-Text PDF

Cyclophilin A regulates secretion of tumour-derived extracellular vesicles.

Transl Oncol 2021 Aug 10;14(8):101112. Epub 2021 May 10.

UCD School of Biomolecular & Biomedical Science, Conway Institute, University College Dublin (UCD), Belfield, Dublin 4, Ireland. Electronic address:

Extracellular Vesicles (EVs) are a heterogenous population of particles that play an important role in cell-cell communication in physiological and pathophysiological situations. In this study we reveal that the peptidyl prolyl isomerase Cyclophilin A (CypA) is enriched in cancer-derived EVs from a range of haematopoietic malignancies. CypA-enriched blood cancer EVs were taken up by normal monocytes independent of EV surface trypsin-sensitive proteins and potently stimulated pro-inflammatory MMP9 and IL-6 secretion. Read More

View Article and Full-Text PDF

Extracellular Vesicles: An Emerging Mechanism Governing the Secretion and Biological Roles of Tenascin-C.

Front Immunol 2021 26;12:671485. Epub 2021 Apr 26.

Mechanoadaptation and Caveolae Biology Lab, Area of Cell and Developmental Biology, Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain.

ECM composition and architecture are tightly regulated for tissue homeostasis. Different disorders have been associated to alterations in the levels of proteins such as collagens, fibronectin (FN) or tenascin-C (TnC). TnC emerges as a key regulator of multiple inflammatory processes, both during physiological tissue repair as well as pathological conditions ranging from tumor progression to cardiovascular disease. Read More

View Article and Full-Text PDF

Ciliary extracellular vesicles are distinct from the cytosolic extracellular vesicles.

J Extracell Vesicles 2021 Apr 27;10(6):e12086. Epub 2021 Apr 27.

Department of Biomedical & Pharmaceutical Sciences Chapman University Irvine California USA.

Extracellular vesicles (EVs) are cell-derived membrane vesicles that are released into the extracellular space. EVs encapsulate key proteins and mediate intercellular signalling pathways. Recently, primary cilia have been shown to release EVs under fluid-shear flow, but many proteins encapsulated in these vesicles have never been identified. Read More

View Article and Full-Text PDF

Nanoalgosomes: Introducing extracellular vesicles produced by microalgae.

J Extracell Vesicles 2021 Apr 27;10(6):e12081. Epub 2021 Apr 27.

University of Ljubljana (UL) Ljubljana Slovene.

Cellular, inter-organismal and cross kingdom communication via extracellular vesicles (EVs) is intensively studied in basic science with high expectation for a large variety of bio-technological applications. EVs intrinsically possess many attributes of a drug delivery vehicle. Beyond the implications for basic cell biology, academic and industrial interests in EVs have increased in the last few years. Read More

View Article and Full-Text PDF

Curcumin-primed human BMSC-derived extracellular vesicles reverse IL-1β-induced catabolic responses of OA chondrocytes by upregulating miR-126-3p.

Stem Cell Res Ther 2021 04 29;12(1):252. Epub 2021 Apr 29.

Department of Orthopaedic Surgery, Experimental Orthopaedics, Centre for Medical Biotechnology (ZMB/Biopark 1), University of Regensburg, Regensburg, Germany.

Background: Curcumin has anti-inflammatory effects and qualifies as a potential candidate for the treatment of osteoarthritis (OA). However, curcumin has limited bioavailability. Extracellular vesicles (EVs) are released by multiple cell types and act as molecule carrier during intercellular communication. Read More

View Article and Full-Text PDF

Extracellular Vesicle Surface Signatures in IPF Patients: A Multiplex Bead-Based Flow Cytometry Approach.

Cells 2021 Apr 28;10(5). Epub 2021 Apr 28.

Respiratory Diseases Unit, Department of Medical and Surgical Sciences & Neurosciences, Siena University Hospital, 53100 Siena, Italy.

Background Extracellular vesicles (EVs) are secreted by cells from their membrane within circulation and body fluids. Knowledge of the involvement of EVs in pathogenesis of lung diseases is increasing. The present study aimed to evaluate the expression of exosomal surface epitopes in a cohort of idiopathic pulmonary fibrosis (IPF) patients followed in two Italian Referral Centres for Interstitial Lung Diseases, comparing them with a group of healthy volunteers. Read More

View Article and Full-Text PDF

Optimal Energy Management Framework for Truck-Mounted Mobile Charging Stations Considering Power Distribution System Operating Conditions.

Sensors (Basel) 2021 Apr 15;21(8). Epub 2021 Apr 15.

School of Electrical and Electronics Engineering, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, Korea.

A high charging demand from many electric vehicles (EVs) at a fixed charging station (FCS) with a limited number of charging poles can increase the waiting time of EVs and yield an abnormal power grid condition. To resolve these challenges, this paper presents an optimization framework in which a mobile charging station (MCS) is dispatched to the overloaded FCS to reduce the number of waiting EVs while maintaining normal power grid operation. Compared to existing MCS scheduling methods that do not consider actual power distribution system operations, the proposed framework takes into account the (i) active/reactive power flow and consumption of EVs, (ii) reactive power capability of MCS, and (iii) voltage quality in power distribution systems. Read More

View Article and Full-Text PDF

Extracellular Vesicle Associated Non-Coding RNAs in Lung Infections and Injury.

Cells 2021 Apr 21;10(5). Epub 2021 Apr 21.

Division of Pulmonary and Critical Care Medicine, Department of Medicine, Boston University Medical Campus 72 E Concord St. R304. Boston, MA 02118, USA.

Extracellular vesicles (EVs) refer to a heterogenous population of membrane-bound vesicles that are released by cells under physiological and pathological conditions. The detection of EVs in the majority of the bodily fluids, coupled with their diverse cargo comprising of DNA, RNA, lipids, and proteins, have led to the accumulated interests in leveraging these nanoparticles for diagnostic and therapeutic purposes. In particular, emerging studies have identified enhanced levels of a wide range of specific subclasses of non-coding RNAs (ncRNAs) in EVs, thereby suggesting the existence of highly selective and regulated molecular processes governing the sorting of these RNAs into EVs. Read More

View Article and Full-Text PDF

Comparative Analysis of Platelet-Derived Extracellular Vesicles Using Flow Cytometry and Nanoparticle Tracking Analysis.

Int J Mol Sci 2021 Apr 7;22(8). Epub 2021 Apr 7.

Center for Biomedical Technology, Department for Biomedical Research, Danube University Krems, 3500 Krems, Austria.

Growing interest in extracellular vesicles (EVs) has prompted the advancements of protocols for improved EV characterization. As a high-throughput, multi-parameter, and single particle technique, flow cytometry is widely used for EV characterization. The comparison of data on EV concentration, however, is hindered by the lack of standardization between different protocols and instruments. Read More

View Article and Full-Text PDF

Biophotonics for diagnostic detection of extracellular vesicles.

Adv Drug Deliv Rev 2021 Jul 19;174:229-249. Epub 2021 Apr 19.

IRCCS Fondazione Don Carlo Gnocchi ONLUS, Via Capecelatro 66, Milan, Italy. Electronic address:

Extracellular Vesicles (EVs) are versatile carriers for biomarkers involved in the pathogenesis of multiple human disorders. Despite the increasing scientific and commercial interest in EV application in diagnostics, traditional biomolecular techniques usually require consistent sample amount, rely on operator-dependent and time- consuming procedures and cannot cope with the nano-size range of EVs, limiting both sensitivity and reproducibility of results. The application of biophotonics, i. Read More

View Article and Full-Text PDF