Macromol Biosci 2020 06 13;20(6):e2000048. Epub 2020 Apr 13.
Macromolecular Chemistry, Faculty of Natural Science II (Chemistry, Physics and Mathematics), Institute of Chemistry, Martin Luther University Halle-Wittenberg, Von-Danckelmann-Platz 4, Halle (Saale), D-06120, Germany.
The synthesis and self-assembly of peptide-polymer conjugates into fibrillar nanostructures are reported, based on the amyloidogenic peptide KLVFF. A strategy for rational synthesis of polymer-peptide conjugates is documented via tethering of the amyloidogenic peptide segment LVFF (Aβ ) and its modified derivative FFFF to the hydrophilic poly(ethylene glycol) monomethyl ether (mPEG) polymer via thio-bromo based "click" chemistry. The resultant conjugates mPEG-LVFF-OMe and mPEG-FFFF-OMe are purified via preparative gel permeation chromatography technique (with a yield of 61% and 64%, respectively), and are successfully characterized via combination of spectroscopic and chromatographic methods, including electrospray ionization time-of-flight mass spectrometry. Read More