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Leonurine protects against influenza A virus infection-induced pneumonia in mice.

Pathog Dis 2021 Sep 20. Epub 2021 Sep 20.

Department of pediatrics, Daqing Oilfield General Hospital, 9 Zhongkang Street, Daqing, 163001, China.

Background: Influenza A virus (H1N1), a swine-origin influenza A virus, causes seasonal epidemics that result in severe illnesses and deaths. Leonurine has been reported to function as an anti-inflammatory agent with protective effects on nervous, urinary and cardiovascular system. However, the therapeutic effects of leonurine on the pneumonia caused by H1N1 infection remains unclear. Read More

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September 2021

Characteristics and clinical potential of a cellularly modified gelatin sponge.

J Appl Biomater Funct Mater 2021 Jan-Dec;19:22808000211035061

Research Center for Translational Medicine, Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, P.R. China.

Background: Human umbilical cord mesenchymal stem cells (HuMSCs) injected directly have been proven effective for improving chronic wounds. However, HuMSCs largely die within 14 days. The aim of study is to establish a cellularly modified gelatin sponge and investigate its characteristics and clinical potential. Read More

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September 2021

Assessment of the diagnostic accuracy and relevance of a novel ELISA system developed for seroepidemiologic surveys of Helicobacter pylori infection in African settings.

PLoS Negl Trop Dis 2021 Sep 9;15(9):e0009763. Epub 2021 Sep 9.

Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Oita, Japan.

Beside diagnostic uncertainties due to the lack of a perfect gold standard test for Helicobacter pylori infection, the diagnosis and the prevalence estimation for this infection encounter particular challenges in Africa including limited diagnostic tools and specific genetic background. We developed and evaluated the accuracy of an enzyme-linked immunosorbent assay (ELISA) system tailored for H. pylori genetics in Africa (HpAfr-ELISA). Read More

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September 2021

The Association Between Mycobacteria-Specific Antigen-Induced Cytokines and Host Response to Latent Tuberculosis Infection Treatment in a Chinese Population.

Front Microbiol 2021 13;12:716900. Epub 2021 Aug 13.

NHC Key Laboratory of Systems Biology of Pathogens, Center for Tuberculosis Research, Institute of Pathogen Biology, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.

Objectives: Exploring biomarkers monitoring latent tuberculosis infection (LTBI) treatment effectiveness would benefit optimizing the therapeutic regimen. This study aims to identify potential mycobacteria-specific antigen-induced cytokines associated with host responses to preventive treatment.

Methods: Based on a randomized controlled trial on LTBI treatment among individuals with chest radiography abnormalities suggestive of prior tuberculosis (TB), the dynamically changed cytokine levels in QuantiFERON-TB Gold In-Tube (QFT) supernatants were estimated during the treatment by bead-based multiplex assays and enzyme-linked immunosorbent assay. Read More

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The Study of Clinical and Biochemical Parameters in Assessing the Response to the Antiviral Therapy in the Chronic Viral Hepatitis B.

Medicina (Kaunas) 2021 Jul 26;57(8). Epub 2021 Jul 26.

Department of Bacteriology-Virology-Parasitology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania.

: Hepatitis B virus infection remains a major public health concern. The interaction between hepatitis B virus (HBV) hepatitis B virus and the host inflammatory response is an important contributing factor driving liver damage and diseases outcomes. The management of chronic hepatitis B virus infection is an area of massive unmet clinical need worldwide. Read More

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Serum Galectin-3 Level Is Positively Associated with Endothelial Dysfunction in Patients with Chronic Kidney Disease Stage 3 to 5.

Toxins (Basel) 2021 Jul 29;13(8). Epub 2021 Jul 29.

School of Medicine, Tzu Chi University, Hualien 97004, Taiwan.

Galectin-3, which is a novel biomarker of cardiovascular stress and related to inflammation, could predict adverse cardiovascular events. However, its relationship with endothelial function in patients with chronic kidney disease (CKD) remains inconclusive. This study aimed to investigate the association between serum galectin-3 levels and endothelial function in patients with stages 3-5 CKD. Read More

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Procollagen C-proteinase enhancer-1 (PCPE-1), a potential biomarker and therapeutic target for fibrosis.

Matrix Biol Plus 2021 Aug 20;11:100062. Epub 2021 Apr 20.

University of Lyon, CNRS, Tissue Biology and Therapeutic Engineering Laboratory, LBTI, UMR5305, F-69367 Lyon, France.

The correct balance between collagen synthesis and degradation is essential for almost every aspect of life, from development to healthy aging, reproduction and wound healing. When this balance is compromised by external or internal stress signals, it very often leads to disease as is the case in fibrotic conditions. Fibrosis occurs in the context of defective tissue repair and is characterized by the excessive, aberrant and debilitating deposition of fibril-forming collagens. Read More

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Low Seroprevalence of SARS-CoV-2 in Rhode Island blood donors during may 2020 as determined using multiple serological assay formats.

BMC Infect Dis 2021 Aug 25;21(1):871. Epub 2021 Aug 25.

New York Blood Center, Lindsley F. Kimball Research Institute, New York, NY, USA.

Background: Epidemic projections and public health policies addressing Coronavirus disease (COVID)-19 have been implemented without data reporting on the seroconversion of the population since scalable antibody testing has only recently become available.

Methods: We measured the percentage of severe acute respiratory syndrome- Coronavirus-2 (SARS-CoV-2) seropositive individuals from 2008 blood donors drawn in the state of Rhode Island (RI). We utilized multiple antibody testing platforms, including lateral flow immunoassays (LFAs), enzyme-linked immunosorbent assays (ELISAs) and high throughput serological assays (HTSAs). Read More

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Immunogenic Potency of a Chimeric Protein Comprising InvH and IpaD against Salmonella and Shigella spp.

Iran J Allergy Asthma Immunol 2021 Aug 7;20(4):484-493. Epub 2021 Aug 7.

Department of Biology, Faculty of Basic Science, Shahed University, Tehran, Iran.

Shigella and Salmonella cause serious problems in many subjects, including young children and the elderly, especially in developing countries. Chimeric proteins carrying immunogens increase immune response. In-silico tools are applied to design vaccine candidates. Read More

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Herd-level contamination of Neospora caninum, Toxoplasma gondii and Brucella in milk of Iranian dairy farms.

Food Microbiol 2021 Dec 23;100:103873. Epub 2021 Jul 23.

Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.

The bulk milk examination is a reliable screening tool for monitoring the quality of milk in the farms. The infection to Neospora caninum, Toxoplasma gondii and Brucella sp. Was evaluated in bulk milk samples of dairy farms in Hamedan province, West part of Iran. Read More

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December 2021

Protective heterologous T cell immunity in COVID-19 induced by the trivalent MMR and Tdap vaccine antigens.

Med (N Y) 2021 Sep 14;2(9):1050-1071.e7. Epub 2021 Aug 14.

Department of Pathology, Brigham and Women's Hospital & Harvard Medical School, Boston, MA 02115, USA.

Background: T cells control viral infection, promote vaccine durability, and in coronavirus disease 2019 (COVID-19) associate with mild disease. We investigated whether prior measles-mumps-rubella (MMR) or tetanus-diphtheria-pertussis (Tdap) vaccination elicits cross-reactive T cells that mitigate COVID-19.

Methods: Antigen-presenting cells (APC) loaded with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), MMR, or Tdap antigens and autologous T cells from COVID-19-convalescent participants, uninfected individuals, and COVID-19 mRNA-vaccinated donors were co-cultured. Read More

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September 2021

miR-124-3p Ameliorates Isoflurane-Induced Learning and Memory Impairment via Targeting STAT3 and Inhibiting Neuroinflammation.

Neuroimmunomodulation 2021 Aug 13:1-7. Epub 2021 Aug 13.

Department of Anesthesiology, Yidu Central Hospital of Weifang, Weifang, China.

Introduction: Substantial evidence has indicated that isoflurane leads to learning and memory impairment. This study was designed to investigate the potential role of microRNA-124-3p (miR-124-3p) in isoflurane-induced learning and memory impairment in rats.

Methods: Spatial learning and memory of rats were estimated by the Morris water maze (MWM) test after the construction of isoflurane-treated models. Read More

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Impact of the Second Epidemic Wave of SARS-CoV-2: Increased Exposure of Young People.

Front Public Health 2021;9:715192. Epub 2021 Jul 26.

UR 7310, Laboratoire de Virologie, Université de Corse, Corte, France.

We aimed to use serological surveillance based on serial cross-sectional sampling of residual sera obtained from clinical laboratories to compare the differences in age and sex profiles of infected persons in the first and second waves of SARS-CoV-2 in Corsica, France. Residual sera were obtained, including samples from individuals of all ages collected for routine screening or clinical management by clinical laboratories. All the sera collected were tested for the presence of anti-SARS-CoV-2 IgG using a kit for semi-quantitative detection of IgG antibodies against the S1 domain of the viral spike protein (ELISA-S). Read More

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Upregulation of pulmonary tissue factor, loss of thrombomodulin and immunothrombosis in SARS-CoV-2 infection.

EClinicalMedicine 2021 Sep 6;39:101069. Epub 2021 Aug 6.

Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, United States.

Background: SARS-CoV-2 infection is associated with thrombotic and microvascular complications. The cause of coagulopathy in the disease is incompletely understood.

Methods: A single-center cross-sectional study including 66 adult COVID-19 patients (40 moderate, 26 severe disease), and 9 controls, performed between 04/2020 and 10/2020. Read More

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September 2021

Sero-epidemiological study of SARS-CoV-2 infection among healthcare personnel in a healthcare department.

Enferm Infecc Microbiol Clin (Engl Ed) 2021 Aug-Sep;39(7):319-325. Epub 2021 May 16.

Servicio de Microbiología, Servicio de Medicina Preventiva, Hospital General Universitario de Alicante, Instituto de Investigación Sanitaria y Biomédica de Alicante (ISABIAL), Alicante, Spain; Comisión de Infección Hospitalaria, Profilaxis y Política Antibiótica, Hospital General Universitario de Alicante, Alicante, Spain.

Objective: Estimate IgG antibody prevalence against SARS-CoV-2 in healthcare personnel (HCP) of a healthcare department (HD).

Method: Prevalence study. The presence of IgG antibodies against SARS-CoV-2 was determined in HCP of the HD. Read More

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Serum aflatoxin B1 antibody titer, percent hemolysis and transaminases in oral submucous fibrosis.

J Oral Maxillofac Pathol 2021 Jan-Apr;25(1):110-117. Epub 2021 May 14.

Department of Oral Medicine and Radiology, Sharad Pawar Dental College and Hospital, Datta Meghe Institute of Medical Sciences (Deemed to be University), Wardha, Maharashtra, India.

Introduction: Areca nut is deeply rooted sociocultural habit in India. Areca nut reported to be infested by fungi during the field and storage conditions. Areca nut alkaloids, nitrosamines, tobacco and aflatoxin are cytotoxic, immunotoxic to red blood cell and epithelial cell. Read More

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Spatiotemporal assessment of post-harvest mycotoxin contamination in rural North Indian food systems.

Food Control 2021 Aug;126:108071

School of Integrative Plant Sciences, Cornell University, Ithaca, NY, USA.

The spatiotemporal trends in aflatoxin B1 (AFB1), fumonisin B1 (FB1), and deoxynivalenol (DON) accumulation were analyzed in a range of food commodities (maize, groundnut, pearl millet, rice, and wheat) in village settings in Unnao, Uttar Pradesh, India. Samples (n = 1549) were collected across six communities and six time points spanning a calendar year and were analyzed for mycotoxins using enzyme-linked immunosorbent assays. AFB1 and FB1 were common across surveyed villages, with moderate to high detection rates (45-75%) observed across commodities. Read More

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Utility of ferritin and inflammatory biomarkers in the diagnosis of different stages of breast cancer.

Saudi Med J 2021 Aug;42(8):825-831

From the Department of Biochemistry (George, Bobby), and from the Department of Medical Oncology (Dubashi), Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India.

Objectives: To assess the utility of ferritin and inflammatory biomarkers in the diagnosis of stages of breast cancer.

Methods: The study consisted of 4 groups of 20 women, including the healthy control. The patients of group 1 comprised of stages I and II, group 2: stage III and group 3: stage IV of the disease. Read More

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Estimating the proportion and IgG antibody response to SARS-CoV-2 in individuals joining a central educational institute from different parts of India by Enzyme-linked immunosorbent assay (ELISA) based serology.

Med J Armed Forces India 2021 Jul 26;77:S366-S372. Epub 2021 Jul 26.

Director & Commandant, Armed Forces Medical College, Pune, India.

Background: Antibody response to SARS-CoV may be estimated to give trends and patterns emerging in a population during an evolving epidemic. The novel coronavirus has opened a new chapter in the history of pandemics and understanding the disease epidemiology.

Methods: The study was a cross-sectional descriptive study. Read More

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Association Between Kidney Function Decline and Baseline TNFR Levels or Change Ratio in TNFR by Febuxostat Chiefly in Non-diabetic CKD Patients With Asymptomatic Hyperuricemia.

Front Med (Lausanne) 2021 12;8:634932. Epub 2021 Jul 12.

Department of Nephrology, Juntendo University Faculty of Medicine, Tokyo, Japan.

The levels of circulating tumor necrosis factor receptor (TNFR) 1 and 2 help predict the future decline of estimated glomerular filtration rate (eGFR) chiefly in patients with diabetes. It has been recently reported that the change ratio in TNFR1 by SGLT2 inhibitor treatment is also related with future GFR decline in patients with diabetes. The aims of this study are to investigate the association between baseline TNFR levels and early change in TNFR levels by the non-purine selective xanthine oxidase inhibitor, febuxostat, and future eGFR decline chiefly in chronic kidney disease (CKD) patients without diabetes. Read More

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First seroprevalence investigation of epizootic haemorrhagic disease virus in Libya.

Open Vet J 2021 Apr-Jun;11(2):301-308. Epub 2021 Jun 21.

Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale", (IZSAM), Teramo, Italy.

Background: Epizootic haemorrhagic disease (EHD) is a vector-borne viral disease of domestic and wild ruminants. Epizootic haemorrhagic disease virus (EHDV) is transmitted by spp. EHDV is a member of the genus within the family. Read More

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Genetic and phenotypic parameters for Aleutian disease tests and their correlations with pelt quality, reproductive performance, packed-cell volume, and harvest length in mink.

J Anim Sci 2021 Aug;99(8)

Department of Animal Science and Aquaculture, Dalhousie University, Truro, Nova Scotia, B2N 5E3, Canada.

Aleutian disease (AD), caused by the Aleutian mink disease virus (AMDV), is a major health concern that results in global economic losses to the mink industry. The unsatisfactory outcome of the culling strategy, immunoprophylaxis, and medical treatment in controlling AD have urged mink farmers to select AD resilient mink based on several detection tests, including enzyme-linked immunosorbent assay (ELISA), counterimmunoelectrophoresis (CIEP), and iodine agglutination test (IAT). However, the genetic analysis of these AD tests and their correlations with pelt quality, reproductive performance, packed-cell volume (PCV), and harvest length (HL) have not been investigated. Read More

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SARS-CoV-2 seroprevalence and associated factors in Manaus, Brazil: baseline results from the DETECTCoV-19 cohort study.

Int J Infect Dis 2021 Jul 14;110:141-150. Epub 2021 Jul 14.

Faculdade de Ciências Farmacêuticas, Universidade Federal do Amazonas, Manaus, Amazonas, Brazil. Electronic address:

Background: Manaus, located in the Brazilian rainforest, has experienced two health system collapses due to the coronavirus disease 2019 (COVID-19) pandemic. However, little is known about which groups among the general population have been most affected.

Methods: A convenience sampling strategy via online advertising recruited 3046 adults between 19 August 2020 and 2 October 2020. Read More

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PGLYRP1 attenuates atherosclerosis by suppressing endothelial cell adhesion.

J Cardiovasc Pharmacol 2021 Jul 9. Epub 2021 Jul 9.

Department of Cardiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People's Republic of China.

Peptidoglycan recognition protein 1 (PGLYRP1) has long been thought to play an important role in infectious and immune diseases. We hypothesized that it might be involved in the pathophysiology of atherosclerotic diseases, which are regarded as chronic inflammatory diseases. Serum PGLYRP1 concentrations were measured in 240 patients with coronary artery disease (CAD) and 209 age- and gender-matched individuals with normal coronary arteries using enzyme-linked immunosorbent assay. Read More

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Effect of Add-On Therapy of Sodium-Glucose Cotransporter 2 Inhibitors and Dipeptidyl Peptidase 4 Inhibitors on Adipokines in Type 2 Diabetes Mellitus.

J Clin Med Res 2021 Jun 25;13(6):355-362. Epub 2021 Jun 25.

Department of Internal Medicine, Gulf Medical University, Ajman, United Arab Emirates.

Background: Excess adiposity is associated with an increased risk of cardiovascular disease due to metabolic changes in the body. Visceral obesity increases the risk of diabetes mellitus through adipocytokines and hence the effective targeting therapies are essential to control obesity in high-risk individuals. The study's main objective was to evaluate the effect of add-on therapy of sodium-glucose cotransporter 2 (SGLT2) inhibitors and dipeptidyl peptidase 4 (DPP4) inhibitors on visceral fat-associated serum adipokines. Read More

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A Comparative Study on Changes in Total and Differential Milk Cell Counts, Activity, and Expression of Milk Phagocytes of Healthy and Mastitic Indigenous Sahiwal Cows.

Front Vet Sci 2021 21;8:670811. Epub 2021 Jun 21.

Lactation and Immuno-Physiology Laboratory ICAR-National Dairy Research Institute, Karnal, India.

Milk somatic cell counts (SCCs) have been used as a gold standard to monitor mammary health as well as an indicator of raw milk quality. The present work was undertaken to compare the changes in the milk SCC, milk differential leukocyte counts (DLCs), phagocytic activity (PA) of milk neutrophils and macrophages (by nitroblue tetrazolium assay), extracellular trap formation (PicoGreen assay) and mRNA expression of various genes in milk neutrophils and macrophages (reverse transcription-polymerase chain reaction), and milk plasma cortisol concentration (enzyme-linked immunosorbent assay) in healthy, subclinical mastitis (SCM), and clinical mastitis (CM) cows. Milk was collected from healthy, SCM, and CM cows grouped based on their SCCs and California mastitis test with eight cows in each group. Read More

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Effects of different lipid emulsions on serum adipokines, inflammatory markers and mortality in critically ill patients with sepsis: A prospective observational cohort study.

Clin Nutr 2021 07 12;40(7):4569-4578. Epub 2021 Jun 12.

Department of Anesthesiology and Reanimation, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.

Background & Aims: Intravenous lipid emulsions in parenteral nutrition may cause different metabolic responses and immune effects in critically ill patients with sepsis. The aim of this study is to investigate the effects of different lipid emulsions on changes in concentrations of adipokine and cytokine and their relationship with mortality in patients.

Methods: Patients enrolled in this prospective, single-center, observational cohort study, were estimated to require more than ten days of parenteral nutrition. Read More

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Lycopene attenuates Staphylococcus aureus-induced inflammation via inhibiting α-Hemolysin expression.

Microbes Infect 2021 Jul 2:104853. Epub 2021 Jul 2.

Department of Clinical Laboratory, the Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, No. 1 Huanghe Road West, Huai'an 223300, Jiangsu, China. Electronic address:

The purpose of this study was to explore the effect of Lycopene in Staphylococcus aureus (S. aureus) (USA 300)-induced inflammation and to elucidate the potential mechanism of its action. The direct effect of Lycopene on S. Read More

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A case-control study to determination FBXW7 and Fetuin-A levels in patients with type 2 diabetes in Iraq.

J Diabetes Metab Disord 2021 Jun 21;20(1):237-243. Epub 2021 Jan 21.

Al-Yarmouk Teaching Hospital- Ministry of Health of Iraq, Baghdad, Iraq.

Background: diabetes mellitus (type 2) is a chronic disease characterized by hyperglycemia resulting either from a deficiency of insulin, or decrease insulin action or both. This study focused on determining the new marker F- box and WD repeat domain containing protein 7 (FBXW7) as well as Fetuin -A and their correlation with other parameters in Diabetes mellitus type 2.

Methods: Sixty diabetes type2 patients were recruited from (National diabetes Center for treatment and Research) at Al Mustansriya University in Iraq during the period (from November 2018-March 2019) with an age range (37-69) years. Read More

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Serum epidermal growth factor-like domain 7 serves as a novel diagnostic marker for early hepatocellular carcinoma.

BMC Cancer 2021 Jul 3;21(1):772. Epub 2021 Jul 3.

Department of Surgery, Liver Cancer Laboratory, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.

Background: Epidermal growth factor-like domain 7 (Egfl7), a recently identified secreted protein, was significantly increased in patients with HCC by our previous studies. However, its efficacy in the diagnosis of early HCC remains unknown. In this study, we therefore evaluate the efficacy of serum Egfl7 for early HCC diagnosis and compare it with alpha-fetoprotein (AFP). Read More

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