32,249 results match your criteria established local


Personalized combination nano-immunotherapy for robust induction and tumor infiltration of CD8 T cells.

Biomaterials 2021 Apr 27;274:120844. Epub 2021 Apr 27.

Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA; Biointerfaces Institute, University of Michigan, Ann Arbor, MI, 48109, USA; Department of Pharmaceutical Sciences, University of Michigan, Ann Arbor, MI, 48109, USA. Electronic address:

Identification of tumor-specific mutations, called neoantigens, offers new exciting opportunities for personalized cancer immunotherapy. However, it remains challenging to achieve robust induction of neoantigen-specific T cells and drive their infiltration into the tumor microenvironment (TME). Here, we have developed a novel polyethyleneimine (PEI)-based personalized vaccine platform carrying neoantigen peptides and CpG adjuvants in a compact nanoparticle (NP) for their spatio-temporally concerted delivery. Read More

View Article and Full-Text PDF

Bone marrow-derived macrophages converted into cancer-associated fibroblast-like cells promote pancreatic cancer progression.

Cancer Lett 2021 May 4. Epub 2021 May 4.

Department of Surgery and Oncology, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan. Electronic address:

Pancreatic ductal adenocarcinoma (PDAC) is characterized by a desmoplastic reaction caused by cancer-associated fibroblasts (CAFs), which provokes treatment resistance. CAFs are newly proposed to be heterogeneous populations with different functions within the PDAC microenvironment. The most direct sources of CAFs are resident tissue fibroblasts and mesenchymal stem cells, however, the origins and functions of CAF subtypes remain unclear. Read More

View Article and Full-Text PDF

Cefepime use: A need for antimicrobial stewardship.

Infect Dis Now 2021 Jan 18. Epub 2021 Jan 18.

Service des maladies infectieuses et tropicales, Unicaen, CHU de Caen Normandie, Normandie université, 14000 Caen, France.

Objectives: Unlike other 3GCs, Cefepime is a cephalosporin that has, in animal model studies, shown a low risk of selecting resistant mutants. It also enables carbapenems to be saved in treatment of Pseudomonasaeruginosa and the CESP group (Citrobacter, Enterobacter, Serratia and Providencia, as well as the genus Klebsiellaaerogenes, Morganella and Hafnia), consequently producing cephalosporinase. We aimed to determine whether its prescription in a French teaching hospital met criteria for proper use. Read More

View Article and Full-Text PDF
January 2021

Characterization of the gut DNA and RNA Viromes in a Cohort of Chinese Residents and Visiting Pakistanis.

Virus Evol 2021 Jan 24;7(1):veab022. Epub 2021 Mar 24.

School of Medicine, Nanjing University of Chinese Medicine, 138 Xianlin Road, Qixia District, Nanjing 210029, China.

Trillions of viruses inhabit the gastrointestinal tract. Some of them have been well-studied on their roles in infection and human health, but the majority remains unsurveyed. It has been established that the composition of the gut virome is highly variable based on the changes of diet, physical state, and environmental factors. Read More

View Article and Full-Text PDF
January 2021

Workplace Bullying Among Junior Doctors in Malaysia: A Multicentre Cross-Sectional Study.

Malays J Med Sci 2021 Apr 21;28(2):142-156. Epub 2021 Apr 21.

Dean's Office, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.

Background: Research suggests that junior doctors often experience workplace bullying, which may have adverse impacts on medical training and delivery of quality healthcare. However, evidence among local population has not been established. The present study aims to examine the prevalence of workplace bullying among Malaysian junior doctors and explore its associated sociodemographic and employment factors. Read More

View Article and Full-Text PDF

Untangling the cortico-thalamo-cortical loop: cellular pieces of a knotty circuit puzzle.

Nat Rev Neurosci 2021 May 6. Epub 2021 May 6.

Department of Physiology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.

Functions of the neocortex depend on its bidirectional communication with the thalamus, via cortico-thalamo-cortical (CTC) loops. Recent work dissecting the synaptic connectivity in these loops is generating a clearer picture of their cellular organization. Here, we review findings across sensory, motor and cognitive areas, focusing on patterns of cell type-specific synaptic connections between the major types of cortical and thalamic neurons. Read More

View Article and Full-Text PDF

ACR Appropriateness Criteria® Staging and Follow-up of Vulvar Cancer.

J Am Coll Radiol 2021 May;18(5S):S212-S228

Specialty Chair, University of Toronto and Sunnybrook Health Sciences Centre, Toronto, Ontario, Canada.

Vulvar cancer is an uncommon gynecologic tumor and one of several human papillomavirus-associated malignancies. Squamous cell carcinoma is the most prevalent histologic subtype of vulvar cancer, accounting for the majority of cases. Imaging plays an important role in managing vulvar cancer. Read More

View Article and Full-Text PDF

Immunogenicity and safety of two quadrivalent influenza vaccines in healthy adult and elderly participants in India - A phase III, active-controlled, randomized clinical study.

Hum Vaccin Immunother 2021 May 6:1-10. Epub 2021 May 6.

Established Pharmaceuticals Division, Abbott Healthcare Products B.V, Weesp, The Netherlands.

: This study was conducted to compare the immunogenicity and safety profile of two quadrivalent influenza vaccines (QIVs) in healthy adults (18-60 years) and elderly (>61 years) participants.: This phase III study was conducted from March 2018 to April 2018 across 12 sites in India. In this randomized, observer-blind, active-controlled study, 480 participants were randomized to receive a single dose of test vaccine (subunit, inactivated influenza vaccine; Influvac® Tetra, Abbott) (n = 240) or reference vaccine (split virion, inactivated influenza vaccine; VaxiFlu-4, Zydus Cadilla Healthcare) (n = 240). Read More

View Article and Full-Text PDF

Morphological and molecular characterization of an Elaeis oleifera (H.B.K) Cortes germplasm collection located in Ucayali, Peru.

PLoS One 2021 6;16(5):e0250445. Epub 2021 May 6.

Estación Experimental Agraria Pucallpa, Dirección de Recursos Genéticos y Biotecnología, Instituto Nacional de Innovación Agraria (INIA), Coronel Portillo, Ucayali, Perú.

The African oil palm (Elaeis guineensis Jacq) is a crop that is widely distributed in tropical regions around the world; however, this crop is subject to limitations such as rapid trunk growth and susceptibility to bud rot and red ring diseases particularly in South America. To overcome these limitations, national breeding and conservation programs have been established, and there is a need to identify parental palms from natural populations of the American oil palm (E. oleifera H. Read More

View Article and Full-Text PDF

Institutionalizing a Regional Model for Improving Quality of Newborn Care at Birth Across Hospitals in Eastern Uganda: A 4-Year Story.

Glob Health Sci Pract 2021 May 5. Epub 2021 May 5.

Makerere University School of Public Health, Kampala, Uganda.

Introduction: Despite the rapid increase in facility deliveries in Uganda, the number of adverse birth outcomes (e.g., neonatal and maternal deaths) has remained high. Read More

View Article and Full-Text PDF

Forgotten Botany: The Politics of Knowledge within the Royal Botanical Garden of New Spain*.

Authors:
Anna Toledano

Ber Wiss 2021 May 6. Epub 2021 May 6.

History of Science, Stanford University, Department of History, 450 Jane Stanford Way, Building 200, Stanford, CA, 94305-2024, USA.

Spanish naturalists established the Viceregal Botanical Garden of New Spain in Mexico City in 1788 to advance agriculture, manufacturing, and medicine. This colonial institution also served the ideological role of cultivating agents of empire. Rather than establish the garden in the already robust tradition of American botany, the Spanish appropriated this space, employing Creole students and servant workers to Europeanize local botanical knowledge through taxonomic colonialism. Read More

View Article and Full-Text PDF

Intratympanic Lipopolysaccharide Elevates Systemic Fluorescent Gentamicin Uptake in the Cochlea.

Laryngoscope 2021 May 6. Epub 2021 May 6.

Research Service, VA Loma Linda Healthcare System, Loma Linda, California, U.S.A.

Objectives/hypothesis: Lipopolysaccharide (LPS), a key component of bacterial endotoxins, activates macrophages and triggers the release of inflammatory cytokines in mammalian tissues. Recent studies have shown that intratympanic injection of LPS simulates acute otitis media (AOM) and results in morphological and functional changes in the inner ear. Here we established an AOM mouse model with LPS to investigate the uptake of ototoxic gentamicin in the inner ear, and elucidated the underlying mechanism by focusing on cochlear inflammation as a result of AOM. Read More

View Article and Full-Text PDF

Study on attractors during organism evolution.

Sci Rep 2021 May 5;11(1):9637. Epub 2021 May 5.

Faculty of Mechanical Engineering, University of Zielona Gora, ul. Szafrana 4, 65-516, Zielona Gora, Poland.

The important question that arises during determining the evolution of organisms is whether evolution should be treated as a continuous process or whether groups of organisms fall into 'local' attractors during evolution. A similar question arises during considering the development of cells after cancer transformation. Answers to these questions can provide a better understanding of how normal and transformed organisms evolve. Read More

View Article and Full-Text PDF

Accelerated transcranial magnetic stimulation (aTMS) to treat depression with treatment switching: study protocol of a pilot, randomized, delayed-start trial.

Pilot Feasibility Stud 2021 May 5;7(1):104. Epub 2021 May 5.

Department of Mood and Anxiety, Institute of Mental Health, 10 Buangkok View, Singapore, 539747, Singapore.

Background: Repetitive transcranial magnetic stimulation (rTMS) is a technique for stimulating brain activity using a transient magnetic field to induce an electrical current in the brain producing depolarization of focal groups of brain cells. TMS is a protocol approved by the U.S. Read More

View Article and Full-Text PDF

Oxidative stress induced by NOX2 contributes to neuropathic pain via plasma membrane translocation of PKCε in rat dorsal root ganglion neurons.

J Neuroinflammation 2021 May 6;18(1):106. Epub 2021 May 6.

Center for Experimental Medicine, the First Affiliated Hospital of Nanchang University, Nanchang, 330006, Jiangxi, China.

Background: Nicotinamide adenine dinucleotide phosphate oxidase 2 (NOX2)-induced oxidative stress, including the production of reactive oxygen species (ROS) and hydrogen peroxide, plays a pivotal role in neuropathic pain. Although the activation and plasma membrane translocation of protein kinase C (PKC) isoforms in dorsal root ganglion (DRG) neurons have been implicated in multiple pain models, the interactions between NOX2-induced oxidative stress and PKC remain unknown.

Methods: A spared nerve injury (SNI) model was established in adult male rats. Read More

View Article and Full-Text PDF

Rhesus monkeys as a translational model for late-onset Alzheimer's disease.

Aging Cell 2021 May 5:e13374. Epub 2021 May 5.

Division of Geriatrics, Department of Medicine, SMPH, Madison, WI, USA.

Age is a major risk factor for late-onset Alzheimer's disease (AD) but seldom features in laboratory models of the disease. Furthermore, heterogeneity in size and density of AD plaques observed in individuals are not recapitulated in transgenic mouse models, presenting an incomplete picture. We show that the amyloid plaque microenvironment is not equivalent between rodent and primate species, and that differences in the impact of AD pathology on local metabolism and inflammation might explain established differences in neurodegeneration and functional decline. Read More

View Article and Full-Text PDF

Parents' and Service Providers' Experiences of Accessing Health Services from an Intellectual Disability Health Team.

J Prim Care Community Health 2021 Jan-Dec;12:21501327211014068

Sydney Children's Hospitals Network (Westmead), Westmead, NSW, Australia.

A pediatric assessment service for children with Intellectual Disability (ID) was established in a culturally diverse region of Sydney, Australia, to meet the health needs of children and young people with ID. This paper reports on parents' and providers' experiences using qualitative and quantitative analysis of surveys. The survey responses from the parents' enumerated their key concerns and the practical help they received from the service. Read More

View Article and Full-Text PDF

Bridging the gap: A new integrated early intervention service for young people with complex mental health issues.

Early Interv Psychiatry 2021 May 4. Epub 2021 May 4.

Faculty of Medicine and Health, University of Sydney, Sydney, Australia.

Aim: Two integrated headspace Early Intervention Teams (hEITs) were established in 2017 to bridge gaps between headspace, the national primary care youth mental health programme in Australia, and the state funded secondary and tertiary mental health services. This study aims to describe functioning and outcomes of patients referred to hEIT over a 6-month period.

Methods: A retrospective file audit was conducted for all patients accepted into the service over a 6-month period in 2018. Read More

View Article and Full-Text PDF

Early evidence of establishment of the tropical bedbug (Cimex hemipterus) in Central Europe.

Med Vet Entomol 2021 May 4. Epub 2021 May 4.

Institute of Parasitology, Biology Centre CAS, České Budějovice, Czech Republic.

In recent decades, the world has witnessed a remarkable resurgence of bedbugs (Hemiptera: Cimicidae). Although populations of the common bedbug, Cimex lectularius L., expanded in temperate regions of its original distribution, the tropical bedbug, C. Read More

View Article and Full-Text PDF

Distractor filtering is affected by local and global distractor probability, emerges very rapidly but is resistant to extinction.

Atten Percept Psychophys 2021 May 4. Epub 2021 May 4.

Center for Mind/Brain Sciences (CIMeC), University of Trento, Trento, Italy.

Effects of statistical learning (SL) of distractor location have been shown to persist when the probabilities of distractor occurrence are equalized across different locations in a so-called extinction phase. Here, we asked whether lingering effects of SL are still observed when a true extinction phase, during which the distractor is completely omitted, is implemented. The results showed that, once established, the effects of SL of distractor location do survive the true extinction phase, indicating that the pattern of suppression in the saliency map is encoded in a form of long-lasting memory. Read More

View Article and Full-Text PDF

Selecting low-carbon technologies and measures for high agricultural carbon productivity in Taihu Lake Basin, China.

Environ Sci Pollut Res Int 2021 May 4. Epub 2021 May 4.

Key Laboratory of Watershed Geographic Sciences, Nanjing Institute of Geography & Limnology, Chinese Academy of Sciences, Nanjing, 210008, China.

In this paper, Delphi method was used to evaluate the low-carbon technologies and measures for high agricultural carbon productivity in Taihu Lake Basin. We established the selecting process and standards and obtained the final list of low-carbon technologies and management measures of high agricultural carbon productivity in Taihu Lake Basin: (1) the initial list of low-carbon technologies and measures of planting industry included 19 items, of which 10 items were included in the final list. The 10 technologies and measures included in the final list were reducing fertilizers, mixed use of organic fertilizer and chemical fertilizer, soil testing and formulated fertilization, application of controlled release fertilizer, deep application of fertilizers, cultivation of new variety, extension of conservation tillage, extension of midseason/alternate drainage, paddy-upland rotation (rice-rape/rice-wheat), and reducing pesticides. Read More

View Article and Full-Text PDF

Low-dose IL-34 has no effect on osteoclastogenesis but promotes osteogenesis of hBMSCs partly via activation of the PI3K/AKT and ERK signaling pathways.

Stem Cell Res Ther 2021 May 4;12(1):268. Epub 2021 May 4.

Department of Orthopedics Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, No. 88, Jiefang Road, Hangzhou, 310009, China.

Background: Inflammatory microenvironment is significant to the differentiation and function of mesenchymal stem cells (MSCs). It evidentially influences the osteoblastogenesis of MSCs. IL-34, a newly discovered cytokine, playing a key role in metabolism. Read More

View Article and Full-Text PDF

Extracellular Polymeric Substance Protects Some Cells in an Biofilm from the Biomechanical Consequences of Treatment with Magainin 2.

Microorganisms 2021 Apr 30;9(5). Epub 2021 Apr 30.

Departments of Biology and Chemistry, Cornell College, Mount Vernon, IA 52314, USA.

Bacterial biofilms have long been recognized as a source of persistent infections and industrial contamination with their intransigence generally attributed to their protective layer of extracellular polymeric substances (EPS). EPS, consisting of secreted nucleic acids, proteins, and polysaccharides, make it difficult to fully eliminate biofilms by conventional chemical or physical means. Since most bacteria are capable of forming biofilms, understanding how biofilms respond to new antibiotic compounds and components of the immune system has important ramifications. Read More

View Article and Full-Text PDF

Cranial Mandibular Fibrosis Syndrome in Adult Farmed Rainbow Trout .

Pathogens 2021 Apr 30;10(5). Epub 2021 Apr 30.

International Centre of Excellence for Aquatic Animal Health, Cefas Weymouth Laboratory, Weymouth, Dorset DT4 8UB, UK.

An unusual condition affecting market size rainbow trout was investigated. This condition was prevalent for several years at low levels but affected a large proportion of stock during 2018 and 2019. Chronic fibrosis affecting cranial tissues and the jaw was observed in samples collected in 2018. Read More

View Article and Full-Text PDF

Delayed manipulation of regeneration within injured peripheral axons.

Neurobiol Dis 2021 May 1:105383. Epub 2021 May 1.

Peripheral Nerve Research Laboratory, Division of Neurology, Department of Medicine and the Neurosciences and Mental Health Institute, University of Alberta, Canada. Electronic address:

While several new translational strategies to enhance regrowth of peripheral axons have been identified, combined approaches with different targets are rare. Moreover, few have been studied after a significant delay when growth programs are already well established and regeneration-related protein expression has waned. Here we study two approaches, Rb1 (Retinoblastoma 1) knockdown that targets overall neuron plasticity, and near nerve insulin acting as a growth factor. Read More

View Article and Full-Text PDF

Establishing local reference intervals for full blood count and white blood cell differential counts in Cape Town, South Africa.

S Afr Med J 2021 Mar 31;111(4):327-332. Epub 2021 Mar 31.

Division of Haematology, Department of Pathology, Faculty of Health Sciences, University of Cape Town, South Africa; National Health Laboratory Service, Groote Schuur Hospital, Cape Town, South Africa.

Background: Accurate laboratory reference intervals (RIs) are essential to differentiate between health and disease. There are variations in haematological indices within populations relating to gender, age, ethnicity and environment. Iron deficiency is common, has a wide range of clinical morbidities and affects red cell indices. Read More

View Article and Full-Text PDF

[Can a single value of a perypheric inflammatory response marker predict the response to BCG?]

Arch Esp Urol 2021 May;74(4):404-410

Servicio de Urología. Hospital Universitario (UVA) Rio Hortega. Valladolid. España.

Objectives: To evaluate the association of neuthophyl/lymphocyte ratio (NLR) and platelet/lymphocyte ratio (PLR) with BCG intravesical responses in patients with initial diagnosis of non/muscle invasive bladder tumor high-risk (NMIBC).

Material And Methods: Retrospective multicenter study on patients with initial diagnosis of high-grade NMIBC treated with BCG between January 2016 and December 2017. Initially a total of 74 patients were collected. Read More

View Article and Full-Text PDF

A Two-Pore Physiologically Based Pharmacokinetic Model to Predict Subcutaneously Administered Different-Size Antibody/Antibody Fragments.

AAPS J 2021 May 3;23(3):62. Epub 2021 May 3.

Department of Pharmaceutical Sciences, School of Pharmacy and Pharmaceutical Sciences, The State University of New York at Buffalo, 455 Pharmacy Building, Buffalo, New York, 14214, USA.

Quantitative modeling of the subcutaneous absorption processes of protein therapeutics is challenging. Here we have proposed a "two-pore" PBPK model that is able to simultaneously characterize plasma PK of different-size protein therapeutics in mice. The skin compartment is evolved to mechanistically account for the absorption pathways through lymph and blood capillaries, as well as local degradation at the SC injection site. Read More

View Article and Full-Text PDF

Aquablation versus holmium laser enucleation of the prostate in the treatment of benign prostatic hyperplasia in medium-to-large-sized prostates (ATHLETE): protocol of a prospective randomised trial.

BMJ Open 2021 May 3;11(5):e046973. Epub 2021 May 3.

Department of Urology, School of Medicine, University of St. Gallen, St Gallen, Switzerland.

Introduction: A novel method for the surgical treatment of benign prostatic hyperplasia (BPH) called Aquablation has become commercially available. Previous studies have been able to show similar functional results when compared with transurethral resection of the prostate and a high efficacy has been demonstrated when this approach is applied to patients with a prostate size of 80-150 cm.Holmium laser enucleation of the prostate (HoLEP) is a well-established procedure in the surgical treatment of BPH in prostate glands larger than 30 mL and a first-line therapy in glands over 80 mL. Read More

View Article and Full-Text PDF

Study protocol of the LARK (TROG 17.03) clinical trial: a phase II trial investigating the dosimetric impact of Liver Ablative Radiotherapy using Kilovoltage intrafraction monitoring.

BMC Cancer 2021 May 3;21(1):494. Epub 2021 May 3.

ACRF Image X Institute, Sydney, NSW, Australia.

Background: Stereotactic Ablative Body Radiotherapy (SABR) is a non-invasive treatment which allows delivery of an ablative radiation dose with high accuracy and precision. SABR is an established treatment for both primary and secondary liver malignancies, and technological advances have improved its efficacy and safety. Respiratory motion management to reduce tumour motion and image guidance to achieve targeting accuracy are crucial elements of liver SABR. Read More

View Article and Full-Text PDF