535 results match your criteria established intravital

Generation of an Iba1-EGFP Transgenic Rat for the Study of Microglia in an Outbred Rodent Strain.

eNeuro 2021 Sep-Oct;8(5). Epub 2021 Sep 7.

Department of Pharmacology, University of Maryland School of Medicine, Baltimore, Maryland 21201

Neuroscience has been transformed by the ability to genetically modify inbred mice, including the ability to express fluorescent markers specific to cell types or activation states. This approach has been put to particularly good effect in the study of the innate immune cells of the brain, microglia. These specialized macrophages are exceedingly small and complex, but also highly motile and mobile. Read More

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September 2021

Luseogliflozin, a SGLT2 Inhibitor, Does Not Affect Glucose Uptake Kinetics in Renal Proximal Tubules of Live Mice.

Int J Mol Sci 2021 Jul 29;22(15). Epub 2021 Jul 29.

Department of Pharmacology, Kagawa University, Takamatsu 760-8521, Kagawa, Japan.

Proximal tubules (PTs) take up most of the glucose in the glomerular filtrate and return it to peritubular capillary blood. Sodium-glucose cotransporter 2 (SGLT2) at the apical membrane takes up glucose into the cell. Glucose then flows across the cells and is transported to the interstitium via glucose transporter 2 (GLUT2) at the basolateral membrane. Read More

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Homogeneous 2D and 3D alignment of cardiomyocyte in dilated cardiomyopathy revealed by intravital heart imaging.

Sci Rep 2021 Jul 19;11(1):14698. Epub 2021 Jul 19.

Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, 565-0871, Japan.

In contrast to hypertrophic cardiomyopathy, there has been reported no specific pattern of cardiomyocyte array in dilated cardiomyopathy (DCM), partially because lack of alignment assessment in a three-dimensional (3D) manner. Here we have established a novel method to evaluate cardiomyocyte alignment in 3D using intravital heart imaging and demonstrated homogeneous alignment in DCM mice. Whilst cardiomyocytes of control mice changed their alignment by every layer in 3D and position twistedly even in a single layer, termed myocyte twist, cardiomyocytes of DCM mice aligned homogeneously both in two-dimensional (2D) and in 3D and lost myocyte twist. Read More

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In vivo dynamic 3D imaging of oocytes and embryos in the mouse oviduct.

Cell Rep 2021 Jul;36(2):109382

Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA. Electronic address:

Developmental biologists have always relied on imaging to shed light on dynamic cellular events. However, processes such as mammalian fertilization and embryogenesis are generally inaccessible for direct imaging. In consequence, how the oviduct (fallopian tube) facilitates the transport of gametes and preimplantation embryos continues to be unanswered. Read More

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Delayed neutrophil recruitment allows nascent Staphylococcus aureus biofilm formation and immune evasion.

Biomaterials 2021 08 2;275:120775. Epub 2021 Apr 2.

Center for Biofilm Engineering, Montana State University, Bozeman, MT, USA; Department of Chemical and Biological Engineering, Montana State University, Bozeman, MT, USA. Electronic address:

Biofilms that form on implanted medical devices cause recalcitrant infections. The early events enabling contaminating bacteria to evade immune clearance, before a mature biofilm is established, are poorly understood. Live imaging in vitro demonstrated that Staphylococcus aureus sparsely inoculated on an abiotic surface can go undiscovered by human neutrophils, grow, and form aggregates. Read More

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The PI3K/Akt/mTOR pathway as a preventive target in melanoma brain metastasis.

Neuro Oncol 2021 Jul 3. Epub 2021 Jul 3.

Clinical Cooperation Unit Neurooncology, German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ), Heidelberg, Germany.

Background: Brain metastases (BM) are a frequent complication of malignant melanoma (MM), with limited treatment options and poor survival. Prevention of BM could be more effective and better tolerated than treating established BM in various conditions.

Methods: To investigate the temporo-spatial dynamics of PI3K/Akt/mTOR (PAM) pathway activation during BM formation and the preventive potential of its inhibition, in vivo molecular imaging with an Akt biosensor was performed, and long-term intravital multiphoton microscopy through a chronic cranial window in mice. Read More

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Impairing flow-mediated endothelial remodeling reduces extravasation of tumor cells.

Sci Rep 2021 Jun 23;11(1):13144. Epub 2021 Jun 23.

Tumor Biomechanics, INSERM UMR_S1109, CRBS, 67000, Strasbourg, France.

Tumor progression and metastatic dissemination are driven by cell-intrinsic and biomechanical cues that favor the growth of life-threatening secondary tumors. We recently identified pro-metastatic vascular regions with blood flow profiles that are permissive for the arrest of circulating tumor cells. We have further established that such flow profiles also control endothelial remodeling, which favors extravasation of arrested CTCs. Read More

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Intravital Imaging of Circulating Red Blood Cells in the Retinal Vasculature of Growing Mice.

Transl Vis Sci Technol 2021 04;10(4):31

Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.

Purpose: To establish a custom-built, high-speed 90 frame-per-second laser-scanning confocal microscope for real-time in vivo retinal imaging of individual flowing red blood cells (RBCs) in retinal vasculature of live mouse model.

Methods: Fluorescently labeled RBCs were injected into mice of different ages (3 to 62 weeks old). Anti-CD31 antibody conjugated with Alexa Fluor 647 was injected to visualize retinal endothelial cells (ECs). Read More

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Subcellular spatio-temporal intravital kinetics of aflatoxin B and ochratoxin A in liver and kidney.

Arch Toxicol 2021 06 18;95(6):2163-2177. Epub 2021 May 18.

Leibniz Research Centre for Working Environment and Human Factors, Technical University Dortmund, Ardeystr. 67, 44139, Dortmund, Germany.

Local accumulation of xenobiotics in human and animal tissues may cause adverse effects. Large differences in their concentrations may exist between individual cell types, often due to the expression of specific uptake and export carriers. Here we established a two-photon microscopy-based technique for spatio-temporal detection of the distribution of mycotoxins in intact kidneys and livers of anesthetized mice with subcellular resolution. Read More

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Effect of Cannabinoid 2 Receptor Modulation on the Peripheral Immune Response in Central Nervous System Injury-Induced Immunodeficiency Syndrome.

Cannabis Cannabinoid Res 2021 Aug 3;6(4):327-339. Epub 2021 Mar 3.

Department of Anesthesia, Pain Management and Perioperative Medicine, Dalhousie University, Halifax, Canada.

Acute central nervous system (CNS) injury, such as stroke, spinal cord injury, or traumatic brain injury can result in dysregulated immune response, and the condition is known as CNS injury-induced immunodeficiency syndrome (CIDS). The endocannabinoid system is an important homeostatic regulator in the CNS and immune system. Activation of cannabinoid 2 receptors (CB2R) on immune cells has been reported to dampen inflammation, suggesting a potential role of CB2R in the peripheral immune response following CNS injury. Read More

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Immunogenic tumor cell death promotes dendritic cell migration and inhibits tumor growth via enhanced T cell immunity.

iScience 2021 May 14;24(5):102424. Epub 2021 Apr 14.

Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Tondabayashi, Osaka 584-8540, Japan.

Immunogenic tumor cell death enhances anti-tumor immunity. However, the mechanisms underlying this effect are incompletely understood. We established a system to induce tumor cell death and investigated its effect on dendritic cell (DC) migration and T cell responses using intravital photolabeling in mice expressing KikGR photoconvertible protein. Read More

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Directional cues in the tumor microenvironment due to cell contraction against aligned collagen fibers.

Acta Biomater 2021 07 7;129:96-109. Epub 2021 May 7.

Department of Cell and Regenerative Biology, University of Wisconsin School of Medicine and Public Health, 1111 Highland Ave. WIMRII, Madison, WI 53705, United States; University of Wisconsin Carbone Cancer Center, 600 Highland Ave, Madison, WI 53705, United States. Electronic address:

It is well established that collagen alignment in the breast tumor microenvironment provides biophysical cues to drive disease progression. Numerous mechanistic studies have demonstrated that tumor cell behavior is driven by the architecture and stiffness of the collagen matrix. However, the mechanical properties within a 3D collagen microenvironment, particularly at the scale of the cell, remain poorly defined. Read More

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Evaluation of a fluorescence endoscope in murine in-vivo auto-fluorescence glioma models.

Ann Anat 2021 Sep 21;237:151746. Epub 2021 Apr 21.

Klinik für Neurochirurgie, Universitätsklinikum des Saarlandes, Kirrberger Straße 1, 66421 Homburg, Saar, Germany.

Introduction: The value of extended and radical resection of high grade gliomas remains controversial, but the neurosurgical procedure is still vital for effective cancer treatment. Fluorescence guided surgery provides aggressive resection within the tumor margins even on microscopic levels. Aim of this study was to evaluate if a new developed fluorescence endoscope can improve intraoperative vision and tumor delineation. Read More

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September 2021

Respiratory Tract Disorders in Fishes.

Ladislav Novotny

Vet Clin North Am Exot Anim Pract 2021 May;24(2):267-292

Finn Pathologists, CVS Group plc, Norfolk, UK; Novopath Ltd, Ceperka, Czech Republic. Electronic address:

"The piscine respiratory system is represented by gills. Gill diseases are extremely common and may be caused by a large variety of etiologic agents. The gills are in direct contact with water and reflect its quality, for example, pollution, and they also must face the presence of biotic agents, such as viruses, bacteria, fungi, and parasites. Read More

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Functional heterogeneity of cytotoxic T cells and tumor resistance to cytotoxic hits limit anti-tumor activity in vivo.

EMBO J 2021 Jun 15;40(11):e106658. Epub 2021 Apr 15.

Dynamics of Immune Responses Unit, Institut Pasteur, Equipe Labellisée Ligue Contre le Cancer, INSERM U1223, Paris, France.

Cytotoxic T cells (CTLs) can eliminate tumor cells through the delivery of lethal hits, but the actual efficiency of this process in the tumor microenvironment is unclear. Here, we visualized the capacity of single CTLs to attack tumor cells in vitro and in vivo using genetically encoded reporters that monitor cell damage and apoptosis. Using two distinct malignant B-cell lines, we found that the majority of cytotoxic hits delivered by CTLs in vitro were sublethal despite proper immunological synapse formation, and associated with reversible calcium elevation and membrane damage in the targets. Read More

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A DARPin targeting activated Mac-1 is a novel diagnostic tool and potential anti-inflammatory agent in myocarditis, sepsis and myocardial infarction.

Basic Res Cardiol 2021 03 15;116(1):17. Epub 2021 Mar 15.

Cardiology and Angiology I, Heart Center Freiburg University, Faculty of Medicine, University of Freiburg, Freiburg, Germany.

The monocyte β-integrin Mac-1 is crucial for leukocyte-endothelium interaction, rendering it an attractive therapeutic target for acute and chronic inflammation. Using phage display, a Designed-Ankyrin-Repeat-Protein (DARPin) was selected as a novel binding protein targeting and blocking the α I-domain, an activation-specific epitope of Mac-1. This DARPin, named F7, specifically binds to activated Mac-1 on mouse and human monocytes as determined by flow cytometry. Read More

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Actually Seeing What Is Going on - Intravital Microscopy in Tissue Engineering.

Front Bioeng Biotechnol 2021 17;9:627462. Epub 2021 Feb 17.

Department of Plastic and Hand Surgery, University Hospital of Erlangen, Friedrich-Alexander University Erlangen-Nürnberg (FAU), Erlangen, Germany.

Intravital microscopy (IVM) study approach offers several advantages over , , and 3D models. IVM provides real-time imaging of cellular events, which provides us a comprehensive picture of dynamic processes. Rapid improvement in microscopy techniques has permitted deep tissue imaging at a higher resolution. Read More

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February 2021

A Novel Mouse Model for Cerebral Venous Sinus Thrombosis.

Transl Stroke Res 2021 Mar 5. Epub 2021 Mar 5.

Laboratory for Vascular Translational Science (LVTS), INSERM UMR 1148, Université de Paris, 75018, Paris, France.

Cerebral venous sinus thrombosis (CVST) is an uncommon cause of stroke resulting in parenchymal injuries associated with heterogeneous clinical symptoms and prognosis. Therefore, an experimental animal model is required to further study underlying mechanisms involved in CVST. This study is aimed at developing a novel murine model suitable and relevant for evaluating injury patterns during CVST and studying its clinical aspects. Read More

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Analysis of B Cell Migration by Intravital Microscopy.

Bio Protoc 2020 Dec 5;10(23):e3842. Epub 2020 Dec 5.

Oncology Research Unit (UIMEO). Hospital de Oncología, Centro Médico Nacional, Instituto Mexicano del Seguro Social (IMSS), Mexico City, Mexico.

During immune responses, B cells home to lymph nodes (LNs), where they encounter antigens. Homing starts with capture and L-selectin-dependent rolling on the activated endothelium of high endothelial venules (HEV). After recognition of chemokines presented on HEV, activation of B cell integrins occurs mediating firm arrest. Read More

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December 2020

BACH2 enforces the transcriptional and epigenetic programs of stem-like CD8 T cells.

Nat Immunol 2021 03 11;22(3):370-380. Epub 2021 Feb 11.

Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.

During chronic infection and cancer, a self-renewing CD8 T cell subset maintains long-term immunity and is critical to the effectiveness of immunotherapy. These stem-like CD8 T cells diverge from other CD8 subsets early after chronic viral infection. However, pathways guarding stem-like CD8 T cells against terminal exhaustion remain unclear. Read More

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Identification and Characterization of Cancer Cells That Initiate Metastases to the Brain and Other Organs.

Mol Cancer Res 2021 04 22;19(4):688-701. Epub 2020 Dec 22.

Clinical Cooperation Unit Neurooncology, German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ), Heidelberg, Germany.

Specific biological properties of those circulating cancer cells that are the origin of brain metastases (BM) are not well understood. Here, single circulating breast cancer cells were fate-tracked during all steps of the brain metastatic cascade in mice after intracardial injection over weeks. A novel two-photon microscopy methodology was developed that allowed to determine the specific cellular and molecular features of breast cancer cells that homed in the brain, extravasated, and successfully established a brain macrometastasis. Read More

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Bridgin connects the outer kinetochore to centromeric chromatin.

Nat Commun 2021 01 8;12(1):146. Epub 2021 Jan 8.

Molecular Mycology Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Center for Advanced Scientific Research (JNCASR), Bangalore, India, 560064.

The microtubule-binding outer kinetochore is coupled to centromeric chromatin through CENP-C, CENP-T, and CENP-U linker pathways originating from the constitutive centromere associated network (CCAN) of the inner kinetochore. Here, we demonstrate the recurrent loss of most CCAN components, including certain kinetochore linkers during the evolution of the fungal phylum of Basidiomycota. By kinetochore interactome analyses in a model basidiomycete and human pathogen Cryptococcus neoformans, a forkhead-associated domain containing protein "bridgin" was identified as a kinetochore component along with other predicted kinetochore proteins. Read More

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January 2021

Tumor cell network integration in glioma represents a stemness feature.

Neuro Oncol 2021 05;23(5):757-769

Neurology Clinic and Neurooncology Program and National Center for Tumor Diseases, University Hospital Heidelberg, Heidelberg, Germany.

Background: Malignant gliomas including glioblastomas are characterized by a striking cellular heterogeneity, which includes a subpopulation of glioma cells that becomes highly resistant by integration into tumor microtube (TM)-connected multicellular networks.

Methods: A novel functional approach to detect, isolate, and characterize glioma cell subpopulations with respect to in vivo network integration is established, combining a dye staining method with intravital two-photon microscopy, Fluorescence-Activated Cell Sorting (FACS), molecular profiling, and gene reporter studies.

Results: Glioblastoma cells that are part of the TM-connected tumor network show activated neurodevelopmental and glioma progression gene expression pathways. Read More

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Effects of in vivo application of an overnight patch containing Allium cepa, allantoin, and pentaglycan on hypertrophic scars and keloids: Clinical, videocapillaroscopic, and ultrasonographic study.

Dermatol Ther 2021 01 28;34(1):e14665. Epub 2020 Dec 28.

Dermatology Unit, Department of Clinical and Molecular Sciences, Polytechnic Marche University, Ancona, Italy.

Several therapeutic approaches have been described for their treatment of hypertrophic scars and keloids, but to date, the optimal treatment has not been established yet. Our in vivo study was conducted to evaluate the effect of a medical device consisting in an adhesive patch containing onion extract (Allium cepa) 10%, allantoin 1%, and pentaglycan 4% (Kaloidon patch) on hypertrophic scars and keloids. Thirty-nine patients with hypertrophic scars and seven patients with keloids were asked to apply an adhesive patch containing Allium cepa, allantoin, and pentaglycan once/day for at least 8 h consecutively, for 24 weeks. Read More

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January 2021

Scratch-induced partial skin wounds re-epithelialize by sheets of independently migrating keratinocytes.

Life Sci Alliance 2021 01 30;4(1). Epub 2020 Nov 30.

Division of Molecular Pathology, Oncode Institute, Netherlands Cancer Institute, Amsterdam, The Netherlands

Re-epithelialization is a crucial process to reestablish the protective barrier upon wounding of the skin. Although this process is well described for wounds where the complete epidermis and dermis is damaged, little is known about the re-epithelialization strategy in more frequently occurring smaller scratch wounds in which structures such as the hair follicles and sweat glands stay intact. To study this, we established a scratch wound model to follow individual keratinocytes in all epidermal layers in the back skin of mice by intravital microscopy. Read More

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January 2021

Development of a clinically relevant ovarian cancer model incorporating surgical cytoreduction to evaluate treatment of micro-metastatic disease.

Gynecol Oncol 2021 02 20;160(2):427-437. Epub 2020 Nov 20.

Program in Immunology, Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, United States of America. Electronic address:

Objectives: Mouse models of ovarian cancer commonly transfer large numbers of tumor cells into the peritoneal cavity to establish experimental metastatic disease, which may not adequately model early metastatic spread from a primary tumor site. We hypothesized we could develop an ovarian cancer model that predictably represents micro-metastatic disease.

Methods: Murine ID8 ovarian cancer cells were transduced to express enhanced luciferase (eLuc) to enable intravital detection of microscopic disease burden and injected beneath the ovarian bursa of C57Bl/6 mice. Read More

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February 2021

Methane Exhalation Can Monitor the Microcirculatory Changes of the Intestinal Mucosa in a Large Animal Model of Hemorrhage and Fluid Resuscitation.

Front Med (Lausanne) 2020 22;7:567260. Epub 2020 Oct 22.

Institute of Surgical Research, University of Szeged, Szeged, Hungary.

Internal hemorrhage is a medical emergency, which requires immediate causal therapy, but the recognition may be difficult. The reactive changes of the mesenteric circulation may be part of the earliest hemodynamic responses to bleeding. Methane is present in the luminal atmosphere; thus, we hypothesized that it can track the intestinal circulatory changes, induced by hemorrhage, non-invasively. Read More

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October 2020

Endotoxinemia Accelerates Atherosclerosis Through Electrostatic Charge-Mediated Monocyte Adhesion.

Circulation 2021 Jan 10;143(3):254-266. Epub 2020 Nov 10.

Institute for Cardiovascular Prevention (IPEK), LMU Munich Hospital, Germany (A.S., A.O.-G., C.W., P. Lemnitzer, J.R.V., C.P., L.P.O., J.W., Y.D., O.S.).

Background: Acute infection is a well-established risk factor of cardiovascular inflammation increasing the risk for a cardiovascular complication within the first weeks after infection. However, the nature of the processes underlying such aggravation remains unclear. Lipopolysaccharide derived from Gram-negative bacteria is a potent activator of circulating immune cells including neutrophils, which foster inflammation through discharge of neutrophil extracellular traps (NETs). Read More

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January 2021

Intravital imaging reveals cell cycle-dependent myogenic cell migration during muscle regeneration.

Cell Cycle 2020 11 1;19(22):3167-3181. Epub 2020 Nov 1.

Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University , Kyoto, Japan.

During muscle regeneration, extracellular signal-regulated kinase (ERK) promotes both proliferation and migration. However, the relationship between proliferation and migration is poorly understood in this context. To elucidate this complex relationship on a physiological level, we established an intravital imaging system for measuring ERK activity, migration speed, and cell-cycle phases in mouse muscle satellite cell-derived myogenic cells. Read More

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November 2020

Visualization of mechanical stress-mediated Ca signaling in the gut using intravital imaging.

Biosci Microbiota Food Health 2020 11;39(4):209-218. Epub 2020 Jun 11.

Department of Immunology, Medical Research Institute, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.

Mechanosensory systems have been implicated in the maintenance of gut homeostasis, but details on the related mechanisms are scarce. Recently, we generated a conditional Ca biosensor yellow cameleon 3.60 (YC3. Read More

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