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Structural characterization of modified whey protein isolates using cold plasma treatment and its applications in emulsion oleogels.

Food Chem 2021 Mar 26;356:129703. Epub 2021 Mar 26.

School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China. Electronic address:

Cold plasma as a green and expeditious tool was used to modify whey protein isolate (WPI) in order to improve its emulsion capability. The emulsion-based oleogels with antibacterial functions were then constructed using the modified WPI. The modified WPI treated with cold plasma under 10 s at 50 W power significantly lowered the oil-water interface tension. Read More

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Association of circulating C-reactive protein and high-sensitivity C-reactive protein with components of sarcopenia: A systematic review and meta-analysis of observational studies.

Exp Gerontol 2021 Apr 10:111330. Epub 2021 Apr 10.

Department of Computer Sciences, Faculty of Mathematics and Natural Sciences, Heinrich Heine Universität, Düsseldorf, Germany.

Background: Sarcopenia, a multi-faceted skeletal muscle disorder in the older population, has poor health outcomes. Some previous observational studies investigated the association between circulating inflammatory markers and sarcopenia components to evaluate chronic inflammation as a risk factor for sarcopenia in the elderly population. Nevertheless, the association between circulating C-reactive protein (CRP) and hs-CRP, as the recognized markers of systemic inflammation and components of sarcopenia, is unclear. Read More

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Bullying in nursing students: A integrative literature review.

Int J Ment Health Nurs 2021 Apr 13. Epub 2021 Apr 13.

Faculty of Medicine and Health Sciences, University of Oviedo, Oviedo, Spain.

The purpose of this integrative review was to analyse the current situation of bullying in nursing students through an exhaustive review of the last 10 years. As the sources of information, we used electronic databases, such as Medline, CINAHL, PubMed, Cochrane, and SciELO, using bullying, nursing students, and clinical environment as search terms. We based the methodology of the study in PRISMA checklist. Read More

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Generation of Fluorescent Versions of Saccharomyces cerevisiae RPA to Study the Conformational Dynamics of Its ssDNA-Binding Domains.

Methods Mol Biol 2021 ;2281:151-168

Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, St. Louis, MO, USA.

Replication protein A (RPA) is an essential single-stranded DNA (ssDNA)-binding protein that sequesters ssDNA and protects it from nucleolytic degradation. The RPA-ssDNA nucleoprotein acts as a hub to recruit over two dozen DNA metabolic enzymes onto ssDNA to coordinate DNA replication, repair, and recombination. RPA functions as a heterotrimer composed of RPA70, RPA32, and RPA14 subunits and has multiple DNA-binding and protein-interaction domains. Read More

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January 2021

Chemical Catalysis Intervening to Histone Epigenetics.

Acc Chem Res 2021 Apr 13. Epub 2021 Apr 13.

Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

ConspectusLife emerges from complicated and sophisticated chemical networks comprising numerous biomolecules (e.g., nucleic acids, proteins, sugars, and lipids) and chemical reactions catalyzed by enzymes. Read More

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Archiving genetically altered animals: a review of cryopreservation and recovery methods for genome edited animals.

Lab Anim 2021 Apr 13:236772211007306. Epub 2021 Apr 13.

Biological Research Facility, Francis Crick Institute, UK.

With the ever-expanding numbers of genetically altered (GA) animals created in this new age of CRISPR/Cas, tools for helping the management of this vast and valuable resource are essential. Cryopreservation of embryos and germplasm of GA animals has been a widely used tool for many years now, allowing for the archiving, distribution and colony management of stock. However, each year brings an array of advances, improving survival rates of embryos, success rates of fertilisation and the ability to better share lines and refine the methods to preserve them. Read More

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An essential clinical dataset intervention for nursing documentation of a pediatric admission history database.

J Pediatr Nurs 2021 Apr 9;59:110-114. Epub 2021 Apr 9.

Old Dominion University, Norfolk, VA, United States of America.

Purpose: The purpose of this study was to improve nursing documentation efficiencies and satisfaction of a pediatric admission history workflow. Secondary aims determined if defining essential data elements was associated with decreased pediatric admission history documentation time, increased dataset completion rate, and increased satisfaction.

Design And Methods: A quasi-experimental between-group difference comparison was conducted for a nurse-led quality improvement study that included implementation of a pediatric essential clinical dataset (ECD) tool for pre/post-intervention analysis of nursing admission history documentation time, dataset completion rate, and satisfaction. Read More

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Clinical concern for necrotising fasciitis: a review of referrals to plastic surgery units at two tertiary centres.

ANZ J Surg 2021 Apr 12. Epub 2021 Apr 12.

Plastic Surgery, Peninsula Health, Melbourne, Victoria, Australia.

Objectives: To evaluate the impact of clinical suspicion on the diagnosis of necrotising fasciitis (NF) in two inpatient plastic surgery units.

Methods: A multicentre retrospective audit of all referrals of adult patients to the inpatient plastic surgery units of two Melbourne metropolitan tertiary centres from 1 February 2018 to 31 December 2018.

Results: A total of 53 patients (37 male, 16 female) were referred to evaluate for NF. Read More

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Forecasting essential childhood cancer drug need: An innovative model-based approach.

Cancer 2021 Apr 12. Epub 2021 Apr 12.

Division of Hematology/Oncology, The Hospital for Sick Children, Toronto, Ontario, Canada.

Background: Childhood cancer outcomes in low-income and middle-income countries have not kept pace with advances in care and survival in high-income countries. A contributing factor to this survival gap is unreliable access to essential drugs.

Methods: The authors created a tool (FOR ECAST) capable of predicting drug quantity and cost for 18 pediatric cancers. Read More

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RAS-NOTECHS: validity and reliability of a tool for measuring non-technical skills in robotic-assisted surgery settings.

Surg Endosc 2021 Apr 12. Epub 2021 Apr 12.

Institute and Clinic for Occupational, Social and Environmental Medicine, University Hospital, LMU Munich, Ziemssenstrasse 1, 80336, Munich, Germany.

Background: Non-technical skills (NTS) are essential for safe surgical practice as they impact workflow and patient outcomes. Observational tools to measure operating room (OR) teams' NTS have been introduced. However, there are none that account for the specific teamwork challenges introduced by robotic-assisted surgery (RAS). Read More

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A Smartphone-Based Self-management Intervention for Bipolar Disorder (LiveWell): User-Centered Development Approach.

JMIR Ment Health 2021 Apr 12;8(4):e20424. Epub 2021 Apr 12.

Department of Psychiatry and Behavioral Sciences, Feinberg School of Medicine, Northwestern University, Chicago, IL, United States.

Background: Bipolar disorder is a serious mental illness that results in significant morbidity and mortality. Pharmacotherapy is the primary treatment for bipolar disorder; however, adjunctive psychotherapy can help individuals use self-management strategies to improve outcomes. Yet access to this therapy is limited. Read More

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In-situ monitoring of xenobiotics using genetically engineered whole-cell-based microbial biosensors: recent advances and outlook.

World J Microbiol Biotechnol 2021 Apr 12;37(5):81. Epub 2021 Apr 12.

Centre for Healthy Brain Ageing, School of Psychiatry, University of New South Wales, 2052, Sydney, NSW, Australia.

Industrialisation, directly or indirectly, exposes humans to various xenobiotics. The increased magnitude of chemical pesticides and toxic heavy metals in the environment, as well as their intrusion into the food chain, seriously threatens human health. Therefore, the surveillance of xenobiotics is crucial for social safety and security. Read More

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ThermoScan: Semi-automatic Identification of Protein Stability Data From PubMed.

Front Mol Biosci 2021 25;8:620475. Epub 2021 Mar 25.

Department of Pharmacy and Biotechnology (FaBiT), University of Bologna, Bologna, Italy.

During the last years, the increasing number of DNA sequencing and protein mutagenesis studies has generated a large amount of variation data published in the biomedical literature. The collection of such data has been essential for the development and assessment of tools predicting the impact of protein variants at functional and structural levels. Nevertheless, the collection of manually curated data from literature is a highly time consuming and costly process that requires domain experts. Read More

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Identification of key immune-related genes and immune infiltration in atrial fibrillation with valvular heart disease based on bioinformatics analysis.

J Thorac Dis 2021 Mar;13(3):1785-1798

Department of Cardiology, First Affiliated Hospital, Guangxi Medical University, Nanning, China.

Background: Atrial fibrillation (AF) is the most common persistent arrhythmia. Valvular heart disease (VHD) and AF frequently coexist. In our study, from performing bioinformatics analysis, we sought to identify immune-related genes (IRGs) and explore the role of immune cell infiltration in AF-VHD in depth, aiming at investigating the potential molecular mechanism and developing new therapeutic targets for AF, including AF-VHD. Read More

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SMIXnorm: Fast and Accurate RNA-Seq Data Normalization for Formalin-Fixed Paraffin-Embedded Samples.

Front Genet 2021 24;12:650795. Epub 2021 Mar 24.

Department of Population and Data Sciences, Quantitative Biomedical Research Center, The University of Texas Southwestern Medical Center, Dallas, TX, United States.

RNA-sequencing (RNA-seq) provides a comprehensive quantification of transcriptomic activities in biological samples. Formalin-Fixed Paraffin-Embedded (FFPE) samples are collected as part of routine clinical procedure, and are the most widely available biological sample format in medical research and patient care. Normalization is an essential step in RNA-seq data analysis. Read More

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The Experimental Composition Improvisation Continua Model: A Tool for Musical Analysis.

Authors:
Alister Spence

Front Psychol 2021 23;12:611536. Epub 2021 Mar 23.

Lecturer in Music, School of Arts and Media, The Faculty of Arts, Design and Architecture, University of New South Wales, Sydney, NSW, Australia.

Among improvisers and composers today there is a resurgence of interest in experimental music (EM) practices that welcome contingency; engaging with unforeseen circumstances as an essential component of the music-making process, and a means to sonic discovery. I propose the (ECIC) as a model with which to better understand these experimental musical works. The historical Experimental Music movement of the 1950s and 60s is briefly revisited, and the jazz tradition included as an essential protagonist; both being important historical movements leading to the formulation of ideas around contingent musical practices. Read More

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PIRACY: An Optimized Pipeline for Functional Connectivity Analysis in the Rat Brain.

Front Neurosci 2021 26;15:602170. Epub 2021 Mar 26.

Animal Imaging and Technology, EPFL, Lausanne, Switzerland.

Resting state functional MRI (rs-fMRI) is a widespread and powerful tool for investigating functional connectivity (FC) and brain disorders. However, FC analysis can be seriously affected by random and structured noise from non-neural sources, such as physiology. Thus, it is essential to first reduce thermal noise and then correctly identify and remove non-neural artifacts from rs-fMRI signals through optimized data processing methods. Read More

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Voltammetry of 7-dehydrocholesterol as a new and useful tool for Smith-Lemli-Opitz syndrome diagnosis.

Talanta 2021 Jul 6;229:122260. Epub 2021 Mar 6.

Charles University, Faculty of Science, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Albertov 6, CZ-128 43 Prague 2, Czech Republic. Electronic address:

7-Dehydrocholesterol is an essential biomarker of Smith-Lemli-Opitz syndrome, a congenital autosomal recessive disorder. This study shows for the first time that electrochemical oxidation of 7-dehydrocholesterol can be used for its voltammetric determination. Two classes of supporting electrolytes in acetonitrile and a mixture of acetonitrile-water were used: inorganic acids known to promote structural changes of steroids and indifferent electrolytes. Read More

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Addressing Challenges in Health Technology Assessment Institutionalization for Furtherance of Universal Health Coverage Through South-South Knowledge Exchange: Lessons From Bhutan, Kenya, Thailand, and Zambia.

Value Health Reg Issues 2021 Apr 7;24:187-192. Epub 2021 Apr 7.

Health Intervention and Technology Assessment Program, Nonthaburi, Thailand. Electronic address:

Health Technology Assessment (HTA), a tool for priority setting, has emerged as a means of ensuring the sustainability of a Universal Health Coverage (UHC) system. However, setting up an effective HTA system poses multiple challenges and knowledge exchange can play a crucial role in helping countries achieve their UHC targets. This article reports the results of the discussion during a preconference session at the 2019 HTAsiaLink Conference, an annual gathering of HTA agencies in Asia, which supports knowledge transfer and exchange among HTA practitioners. Read More

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Clinical care of neonates undergoing opioid withdrawal in the immediate postpartum period.

Neurotoxicol Teratol 2021 Apr 7:106978. Epub 2021 Apr 7.

Department of Pediatrics, Division of Neonatal-Perinatal Medicine, Penn State College of Medicine, Hershey, PA, USA.

As the opioid epidemic escalates in westernized countries around the world, chronic opioid use during pregnancy has become a growing public health issue. There are increasing concerns that chronic maternal opioid use might adversely affect the developing fetal brain. Furthermore, the sudden discontinuation of the trans-placental opioid supply at birth puts newborns at acute risk for neonatal opioid withdrawal syndrome (NOWS). Read More

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Lipids: biomarkers of healthy aging.

Biogerontology 2021 Apr 10. Epub 2021 Apr 10.

Department of Medical Sciences, iBiMED - Institute of Biomedicine, University of Aveiro, 3810-193, Aveiro, Portugal.

Life expectancy, and longevity have been increasing in recent years. However, this is, in most cases, accompanied by age-related diseases. Thus, it became essential to better understand the mechanisms inherent to aging, and to establish biomarkers that characterize this physiological process. Read More

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Concussion in para sport: the first position statement of the Concussion in Para Sport (CIPS) Group.

Br J Sports Med 2021 Apr 9. Epub 2021 Apr 9.

Para Football Foundation, Arnhem, The Netherlands.

Concussion is a frequent injury in many sports and is also common in para athletes. However, there is a paucity of concussion research related to para sport, and prior International Concussion in Sport (CIS) consensus papers have not substantively addressed this population. To remedy this and to improve concussion care provided to para athletes, the concussion in para sport (CIPS) multidisciplinary expert group was created. Read More

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Bioinformatic pipelines combining denoising and clustering tools allow for more comprehensive prokaryotic and eukaryotic metabarcoding.

Mol Ecol Resour 2021 Apr 9. Epub 2021 Apr 9.

MARBEC, Ifremer, Univ. Montpellier, IRD, CNRS, Sète, France.

Environmental DNA metabarcoding is a powerful tool for studying biodiversity. However, bioinformatic approaches need to adjust to the diversity of taxonomic compartments targeted as well as to each barcode gene specificities. We built and tested a pipeline based on read correction with DADA2 allowing analysing metabarcoding data from prokaryotic (16S) and eukaryotic (18S, COI) life compartments. Read More

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Merging NiH Catalysis and Inner-Sphere Metal-Nitrenoid Transfer for Hydroamidation of Alkynes.

J Am Chem Soc 2021 Apr 9. Epub 2021 Apr 9.

Center for Catalytic Hydrocarbon Functionalizations, Institute for Basic Science, Daejeon 34141, South Korea.

The formal hydroamination/hydroamidation utilizing metal hydride is an appealing synthetic tool for the construction of valuable nitrogen-containing compounds from unsaturated hydrocarbons. While significant advances have been made for the functionalizations of alkenes in this realm, the direct hydroamidation of alkynes remains rather limited due to the high feasibility of the key metal-alkenyl intermediate to choose other reaction pathways. Herein, we report a NiH-catalyzed strategy for the hydroamidation of alkynes with dioxazolones, which allows convenient access to synthetically useful secondary enamides in ()--Markovnikov or Markovnikov selectivity. Read More

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DeepDTAF: a deep learning method to predict protein-ligand binding affinity.

Brief Bioinform 2021 Apr 8. Epub 2021 Apr 8.

School of Computer Science and Engineering, Central South University, China.

Biomolecular recognition between ligand and protein plays an essential role in drug discovery and development. However, it is extremely time and resource consuming to determine the protein-ligand binding affinity by experiments. At present, many computational methods have been proposed to predict binding affinity, most of which usually require protein 3D structures that are not often available. Read More

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Code Review as a Simple Trick to Enhance Reproducibility, Accelerate Learning, and Improve the Quality of Your Team's Research.

Am J Epidemiol 2021 Apr 8. Epub 2021 Apr 8.

Department of Epidemiology and Biostatistics, University of California San Francisco, San Francisco, CA.

Programming for data wrangling and statistical analysis is an essential technical tool of modern Epidemiology, yet many Epidemiologists receive limited formal training in strategies to optimize the quality of our code. In complex projects, coding mistakes are easy to make, even for skilled practitioners. Such mistakes can lead to invalid research claims that reduce the credibility of the field. Read More

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Digital image processing to detect subtle motion in stony coral.

Sci Rep 2021 Apr 8;11(1):7722. Epub 2021 Apr 8.

Department of Aeronautics and Astronautics, University of Washington, Seattle, WA, 98195-2400, USA.

Coral reef ecosystems support significant biological activities and harbor huge diversity, but they are facing a severe crisis driven by anthropogenic activities and climate change. An important behavioral trait of the coral holobiont is coral motion, which may play an essential role in feeding, competition, reproduction, and thus survival and fitness. Therefore, characterizing coral behavior through motion analysis will aid our understanding of basic biological and physical coral functions. Read More

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Spontaneous and deliberate creative cognition during and after psilocybin exposure.

Transl Psychiatry 2021 Apr 8;11(1):209. Epub 2021 Apr 8.

Department of Neuropsychology and Psychopharmacology, Faculty of Psychology and Neuroscience, Maastricht University, P.O. Box 616, 6200 MD, Maastricht, the Netherlands.

Creativity is an essential cognitive ability linked to all areas of our everyday functioning. Thus, finding a way to enhance it is of broad interest. A large number of anecdotal reports suggest that the consumption of psychedelic drugs can enhance creative thinking; however, scientific evidence is lacking. Read More

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Study on the effect of education for insulin injection in diabetic patients with new simulation tools.

Medicine (Baltimore) 2021 Apr;100(14):e25424

Department of Endocrinology and Metabolism, Wuzhou Worker Hospital, Gao Di Road.

Background: Insulin is an essential therapy for treating diabetes, but many patients lack standard insulin injection skills.

Purpose: We developed a standard training procedure based on a new simulation tool. Then we conducted a study to investigate the effect of this standard training on the ability of diabetic patients to inject themselves with insulin. Read More

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Applying model approaches in non-model systems: A review and case study on coral cell culture.

PLoS One 2021 8;16(4):e0248953. Epub 2021 Apr 8.

Life Science and Engineering, Virginia Commonwealth University, Richmond, Virginia, United States of America.

Model systems approaches search for commonality in patterns underlying biological diversity and complexity led by common evolutionary paths. The success of the approach does not rest on the species chosen but on the scalability of the model and methods used to develop the model and engage research. Fine-tuning approaches to improve coral cell cultures will provide a robust platform for studying symbiosis breakdown, the calcification mechanism and its disruption, protein interactions, micronutrient transport/exchange, and the toxicity of nanoparticles, among other key biological aspects, with the added advantage of minimizing the ethical conundrum of repeated testing on ecologically threatened organisms. Read More

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