11,242 results match your criteria epididymal sperm


Dried bovine placenta improves spermatozoa count in a rat model of male reproductive aging.

Vet World 2021 Jun 21;14(6):1602-1607. Epub 2021 Jun 21.

Sains Veteriner Magister, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.

Background And Aim: In the male reproductive system, the aging process can lead to infertility. Recently, placenta and its derivatives have been researched as regenerative agents. This study aimed to describe the basic components of dried bovine placenta powder and its potential effects as a regenerative agent in a rat model of male reproductive aging with D-galactose induction. Read More

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The evolutionarily conserved gene, Fam114a2, is dispensable for fertility in mouse.

Reprod Biol 2021 Jul 24;21(3):100531. Epub 2021 Jul 24.

The First Affiliated Hospital of USTC, Hefei National Laboratory for Physical Sciences at Microscale, School of Life Sciences, University of Science and Technology of China, Hefei 230027, China. Electronic address:

Family with sequence similarity 114 member A2 (Fam114a2) is sperm binding protein that is highly conserved in mammals with homologs both in fungi and plants. Previous studies have demonstrated that miR-762 and P63 are two crucial players of spermatogenesis, and CricFM114A2 regulates their expression. Thus, the current study was focused on describing the role of Fam114a2 in spermatogenesis by generating Fam114a2 knockout (Fam114a2) mice using CRISPR/Cas9 genome editing techniques. Read More

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Relationship of sperm motility with clinical outcome of percutaneous epididymal sperm aspiration-intracytoplasmic sperm injection in infertile males with congenital domestic absence of vas deferens: a retrospective study.

Zygote 2021 Jul 27:1-5. Epub 2021 Jul 27.

Department of Infertility, Jiangsu Huaian Maternity and Children Hospital, Huaian, China.

Congenital domestic absence of vas deferens (CBAVD) is a common factor in male infertility, and percutaneous epididymal sperm aspiration (PESA) combined with intracytoplasmic sperm injection (ICSI) is a primary clinical treatment, but the effect of the sperm obtained on pregnancy outcome remains to be explored. This study aimed to investigate the relationship between sperm motility with clinical outcome of PESA-ICSI in infertile males with CBAVD. A cohort of 110 couples was enrolled. Read More

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Comparative iTRAQ proteomics identified proteins associated with sperm maturation between yak and cattleyak epididymis.

BMC Vet Res 2021 Jul 26;17(1):255. Epub 2021 Jul 26.

Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization (Southwest Minzu University), Ministry of Education, Chengdu, 610041, Sichuan, China.

Background: During maturation, spermatozoa acquire motility and fertilizing capacity as they transit through the epididymis. In recent years, two-dimensional gel electrophoresis has been employed in proteomics studies conducted in rat, boar and human. However, there has not been a complete information regarding the proteins associated with sperm maturation in the epididymis. Read More

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A Possible Highway System for the Rapid Delivery of Sperm from the Testis to the Penis in the Naked Mole-Rat, Heterocephalus glaber.

J Morphol 2021 Jul 23. Epub 2021 Jul 23.

Department of Medical, Biosciences, University of the Western Cape, Bellville, South Africa.

Gametogenesis is suppressed in most members of the eusocial naked mole-rat (NMR) colony, while the queen selects mainly one breeding male during her life span. Recently, it was reported that the NMR testicular organization seems to produce spermatozoa on demand after suppression of spermatogenesis during most of gestation. A Sertoli cell "pump" is then used to flush the spermatozoa into short tubuli recti and simplified rete testis to reach the excurrent duct system. Read More

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A novel mouse line with epididymal initial segment-specific expression of Cre recombinase driven by the endogenous Lcn9 promoter.

PLoS One 2021 26;16(7):e0254802. Epub 2021 Jul 26.

Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, China.

Spermatozoa released from testes undergo a maturation process and acquire the capacity to fertilize ova through epididymal transit. The epididymis is divided into four regions, each with unique morphology, gene profile, luminal microenvironment and distinct function. To study the functions of relevant genes in the epididymal initial segment (IS), a novel IS-specific mouse model, Lcn9-Cre knock-in (KI) mouse line was generated via CRISPR/Cas9 technology. Read More

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Assessment of MUSASHI 1 and MUSASHI 2 expression in spermatozoa and testicular tissue.

Andrologia 2021 Jul 26:e14187. Epub 2021 Jul 26.

Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.

MUSASHI (MSI) family plays the main role in the spermatogenesis process. The purpose of this study was the assessment of sperm MSI1 and MSI2, and sperm functional tests in infertile men (n = 30) with varicocele and fertile men (n = 30). Furthermore, MSI1 and MSI2 proteins were assessed in testicular tissue of azoospermic men (n = 9) as well as epididymal spermatozoa and testis of mice. Read More

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Wild Garlic Allium triquetrum L. Alleviates Lead Acetate-Induced Testicular Injuries in Rats.

Biol Trace Elem Res 2021 Jul 26. Epub 2021 Jul 26.

Laboratory of Animal Ecophysiology, Department of Biology, Faculty of Sciences, University of Badji Mokhtar-Annaba, 23000, Annaba, Algeria.

The current study investigates the potential alleviating activity of bulbs (B) and leaves (L) of Allium triquetrum aqueous extract (ATE) on repro-toxicity induced by lead acetate (Pb) in male Wistar rats administrated orally for 3 consecutive weeks. Eighteen groups of rats were divided into the control, Pb (500 mg/kg body weight/day), positive controls of B and L (2 g, 3 g, 4 g, 6 g/kg body weight/day), in addition to four mixtures of each of Pb-B (Pb-B1, Pb-B2, Pb-B3, Pb-B4) and Pb-L (Pb-L1, Pb-L2, Pb-L3, Pb-L4). The two extracts were subjected to phytochemical screening and HPLC analysis. Read More

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Morphological analysis of interstitial cells in murine epididymis using light microscopy and transmission electron microscopy.

Acta Histochem 2021 Jul 20;123(6):151761. Epub 2021 Jul 20.

Division of Microscopic and Developmental Anatomy, Department of Anatomy, Kurume University School of Medicine, Kurume, 830-0011, Japan.

Smooth muscle contraction of the epididymis plays an important role in sperm transport. Although PDGFRα-positive interstitial cells (PDGFRα (+) ICs) are thought to be involved in controlling smooth muscle movement via intercellular signaling, they have not yet been reported to date in the epididymis. Therefore, we aimed to investigate the morphological characteristics of PDGFRα (+) ICs in the interstitial space of the murine epididymis. Read More

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Pharmacological Inactivation of CatSper Blocks Sperm Fertilizing Ability Independently of the Capacitation Status of the Cells: Implications for Non-hormonal Contraception.

Front Cell Dev Biol 2021 6;9:686461. Epub 2021 Jul 6.

Instituto de Biología y Medicina Experimental (IByME-CONICET), Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina.

Cation channel of sperm (CatSper), the main sperm-specific Ca channel, plays a key role in mammalian fertilization, and it is essential for male fertility, becoming an attractive target for contraception. Based on this, in the present work, we investigated the effects of CatSper inactivation on and sperm fertilizing ability and the mechanisms underlying such effects. Exposure of cauda epididymal mouse sperm to different concentrations (1-20 μM) of the potent CatSper inhibitor HC-056456 (HC) during capacitation showed no effects on sperm viability but significantly affected Ca entry into the cells, progressive motility, protein tyrosine phosphorylation, induced acrosome reaction, and hyperactivation, as well as the sperm's ability to fertilize cumulus oocyte complexes and zona-free eggs. Read More

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induced sperm DNA damage on the experimentally infected rats.

J Parasit Dis 2021 Jun 10;45(2):351-358. Epub 2020 Nov 10.

Department of Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, P.O. Box: 65157838736, Hamadan, Iran.

, as an obligate protozoan parasite, can infect a wide variety of animals as well as human. As some studies have shown, toxoplasmosis decreases the fertility potency in different hosts, so there is a necessity for studies to determine the effects of on reproductive system. Therefore, this project was aimed to investigate the effect of toxoplasmosis on the male reproductive system and sperm DNA integrity. Read More

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Quercetin and vitamin E attenuate diabetes-induced testicular anomaly in Wistar rats via the mitochondrial-mediated apoptotic pathway.

Andrologia 2021 Jul 18:e14185. Epub 2021 Jul 18.

Laboratories for Biomembrane Research and Biotechnology, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria.

The role of quercetin and vitamin E treatment against streptozotocin (STZ)-induced testicular abnormalities in diabetic rats and the possible mechanism of action they use for protection were investigated. Diabetes was induced by STZ (45 mg/kg i.p. Read More

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Protective effects of L-carnitine on reproductive capacity in rats with diabetes.

J Physiol Pharmacol 2021 Feb 13;72(1). Epub 2021 Jul 13.

Department of Gastroenterology, Binzhou Medical University Hospital, Binzhou, China.

To explore the protective effects of L-carnitine on erectile function and reproductive function in rats with diabetes. A total of 60 male diabetes mellitus induced-erectile dysfunction (DMED) rats were randomly divided into three groups, 20 rats in each group. The blank group was fed normally, the control group was fed with 0. Read More

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February 2021

Er-Xian decoction exhibits protective activity in a rat model of asthenospermia through a mechanism associated with DJ-1 protein upregulation.

J Physiol Pharmacol 2021 Feb 13;72(1). Epub 2021 Jul 13.

Department of Andrology, Yunnan Provincial Hospital of Traditional Chinese Medicine Hospital; The First Affiliated Hospital, Yunnan University of Chinese Medicine, Kunming, China.

Asthenozoospermia (AZS) remains a significant clinical problem of male factor infertility. Er-Xian decoction (EXD) is a traditional Chinese medicine with potent antioxidant activity to treat AZS. To investigate the protective effects of EXD on sperm motility and deglycase (DJ)-1 expression in AZS model rats. Read More

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February 2021

Anti-CD52 Therapy for Multiple Sclerosis: An Update in the COVID Era.

Immunotargets Ther 2021 7;10:237-246. Epub 2021 Jul 7.

Department of Experimental and Clinical Pharmacology, Centre for Preclinical Research, Medical University of Warsaw, Warsaw, Poland.

CD52 is a small surface glycoprotein composed of 12 amino acids. CD52 is found mostly on the surface of mature immune cells, such as lymphocytes, monocytes, eosinophils, and dendritic cells, as well as the male genital tract: within the epididymis and on the surface of mature sperm. Low CD52 expression is also found in neutrophils. Read More

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Knockout of the family with sequence similarity 181, member A (Fam181a) gene does not impair spermatogenesis or male fertility in the mouse.

Reprod Fertil Dev 2021 Jul 13. Epub 2021 Jul 13.

Family with sequence similarity 181 (Fam181) is a gene family with two paralogues (Fam181a and Fam181b) found among vertebrates. Fam181a exhibits dynamic and stage-specific expression during murine embryo development. Furthermore, searching in the National Center for Biotechnology Information database revealed predominant expression of Fam181a in mouse and human testes, implying that it may have essential roles in spermatogenesis. Read More

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Successful blastocyst production by intracytoplasmic injection of sperm after in vitro maturation of follicular oocytes obtained from immature female squirrel monkeys (Saimiri boliviensis).

J Reprod Dev 2021 Jul 9. Epub 2021 Jul 9.

Graduate School of Agricultural Science, Utsunomiya University, Tochigi 321-8505, Japan.

Advanced reproductive technologies are being applied for the propagation of squirrel monkeys, to ensure their preservation as a genetic resource and the effective use of their gametes in the future. In the present study, oocytes and spermatozoa were collected from live squirrel monkeys, following which piezo intracytoplasmic sperm injection (ICSI) was performed using these gametes. Follicular development was induced by administering equine chorionic gonadotropin (eCG) containing inhibin antiserum to an immature squirrel monkey female. Read More

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Simulated physiological oocyte maturation (SPOM) improves developmental competence of in vitro produced goat embryos.

Theriogenology 2021 Jun 23;172:193-199. Epub 2021 Jun 23.

Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Jabalpur, Madhya Pradesh, India.

The effect of simulated physiological oocyte maturation on the developmental competence, reactive oxygen species production and apoptosis rate of in vitro produced goat embryos were studied in the present experiment. Oocytes and spermatozoa were recovered from ovaries and epididymis, respectively, procured from a local small animal abattoir. The oocytes aspirated from the ovaries were allocated into two groups, control (subjected to routine in vitro maturation, fertilization and culture) and simulated physiological oocyte maturation (SPOM) group (subjected to prematuration, followed by routine in vitro maturation, fertilization and culture). Read More

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Improved quality and fertilizability of cryopreserved buffalo spermatozoa with the supplementation of methionine sulfoxide reductase A.

Andrology 2021 Jul 10. Epub 2021 Jul 10.

Division of Biochemistry, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India.

Background: The excessive reactive oxygen species produced during semen-freezing and -thawing damage the macromolecules resulting in impairment of cellular functions. Proteins are the primary targets of oxidative damage, wherein methionine residues are more prone to oxidation and get converted into methionine sulfoxide, thus affecting the protein function. The methionine sulfoxide reductase A (MsrA) catalyzes the conversion of methionine sulfoxide to methionine and restores the functionality of defective proteins. Read More

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Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains.

Sci Rep 2021 07 8;11(1):14149. Epub 2021 Jul 8.

RIKEN BioResouce Research Center, Tsukuba, Ibaraki, 305-0074, Japan.

Cryopreservation of mouse spermatozoa is widely used for the efficient preservation and safe transport of valuable mouse strains. However, the current cryopreservation method requires special containers (plastic straws), undefined chemicals (e.g. Read More

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Evaluation of testicular glycogen storage, FGF21 and LDH expression and physiological parameters of sperm in hyperglycemic rats treated with hydroalcoholic extract of Securigera Securidaca seeds, and Glibenclamide.

Reprod Biol Endocrinol 2021 Jul 7;19(1):104. Epub 2021 Jul 7.

Department of Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.

Structural and physiological changes in sperm and semen parameters reduce fertility in diabetic patients. Securigera Securidaca (S. Securidaca) seed is a herbal medicine with hypoglycemic, antioxidant, and anti-hypertensive effects. Read More

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Effects of quetiapine administration on sperm quality and testicular histology.

Drug Chem Toxicol 2021 Jul 7:1-9. Epub 2021 Jul 7.

Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Anadolu University, Eskisehir, Turkey.

Quetiapine is one of the most commonly prescribed antipsychotics to treat schizophrenia in adults, in particular. In this study, quetiapine's effects were assessed on healthy sperm production in rats at repeated-pharmacological doses. Additionally, the effects of quetiapine on oxidative status and hormonal balance were also evaluated in rats. Read More

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Bee bread mitigates down-regulation of steroidogenic genes, decreased spermatogenesis, and epididymal oxidative stress in male rats fed with high-fat diet.

Am J Physiol Endocrinol Metab 2021 07 6. Epub 2021 Jul 6.

Department of Physiology, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia.

Background: The pituitary-gonadal axis plays an important role in steroidogenesis and spermatogenesis and by extension, fertility.

Objectives: The aim of this study was to investigate the protective role of bee bread, which is a natural bee product, against obesity-induced decreases in steroidogenesis and spermatogenesis.

Methods: Thirty-two adult male Sprague-Dawley rats weighing between 200-300 g were divided into four groups (n=8/group), namely: normal control (NC), high-fat diet (HFD), HFD plus bee bread administered concurrently for 12 weeks (HFD+B), HFD plus orlistat administered concurrently for 12 weeks (HFD+O) groups. Read More

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A Comparative study on the effects of Yaji (Suya Meat sauce) and its spice constituents on the male reproductive profile of adult male Sprague Dawley rats.

JBRA Assist Reprod 2021 Feb 2;25(1). Epub 2021 Feb 2.

Department of Anatomy, College of Medicine, University of Lagos, Lagos State, Nigeria.

Objective: This comparative study was designed to analyze the potential effects of Yaji (suya meat sauce) and its composite spices on male fertility based on testicular histology, serum testosterone level, and semen analysis parameters.

Methods: The study included 70 adult male Sprague Dawley rats with an average weight of 120 g. They were divided into two experimental study groups, respectively analyzed for 28 and 56 days. Read More

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February 2021

Effect of extract on reproductive damages in streptozotocin induced diabetic male rats.

J Diabetes Metab Disord 2021 Jun 22;20(1):77-85. Epub 2021 Jan 22.

Neuroscience Research Center, Qom University of Medical Sciences, Pardis Campus, Ghadir Blvd, Qom, Iran.

Purpose: Infertility is one of the systemic problems in diabetic men. The purpose of the present study is investigation of the effects of the () hydro-alcoholic extract on the reproductive system damages in diabetic male rats.

Methods: Twenty four male rats were randomly assigned into 4 groups ( = 6); i. Read More

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Effect of melatonin supplementation during IVM of dromedary camel oocytes (Camelus dromedarius) on their maturation, fertilization, and developmental rates in vitro.

Theriogenology 2021 May 23;172:187-192. Epub 2021 May 23.

Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.

The positive impact of melatonin on in vitro embryo production (IVEP) has been reported in many domestic species; however, no studies have been carried out in camelids. We aimed to evaluate the effects of melatonin supplementation in maturation media on in vitro maturation, fertilization, and preimplantation embryo development of dromedary camel oocytes (experiment 1). We also evaluated the concentrations of total antioxidant capacity (TAC), and malondialdehyde (MDA) in the IVM spent medium in relation to melatonin supplementation. Read More

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Protective effects of olive leaf extract against reproductive toxicity of the lead acetate in rats.

Environ Sci Pollut Res Int 2021 Jul 3. Epub 2021 Jul 3.

Department of Medical Laboratory Techniques, Al-maarif University College, Al-Anbar, Ramadi, Iraq.

Lead acetate (PbAc) is one of the toxic metals in the environment which causes many effects on different organs of the body. And due to the importance of the olive tree, with its healthy and protective elements against many diseases, the leaf extract of this tree was chosen in our study. Therefore, the aim of this study was to investigate the role of olive leaf (Olea europea L. Read More

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Anti-fertility effect of levonorgestrel and/or quinestrol on striped field mouse (Apodemus agrarius): evidence from both laboratory and field experiments.

Integr Zool 2021 Jul 3. Epub 2021 Jul 3.

Shaanxi Key Laboratory for Animal Conservation, Shaanxi Institute of Zoology, Xi'an, China.

The effect of combined levonorgestrel (P) and quinestrol (E) on the fertility of striped field mouse (Apodemus agrarius) has not been evaluated. We performed a series of experiments in both the laboratory and field to assess the effect of P and/or E on the fertility of A. agrarius. Read More

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Assessment of tebuconazole exposure on bovine testicular cells and epididymal spermatozoa.

Acta Vet Hung 2021 Jul 1. Epub 2021 Jul 1.

2Department of Artificial Insemination and Reproduction, Faculty of Veterinary Medicine, Kirikkale University, Kirikkale, Turkey.

This study is the first to investigate the effects of tebuconazole (TEB) on the physiological functions of bovine testicular cells and epididymal spermatozoa. Motility and plasma membrane integrity of spermatozoa exposed to TEB (0.001-100 µM) were evaluated at different incubation times (0-6 h), while TEB-induced spermiotoxicity was assessed after 24 h in cell cultures. Read More

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Epididymosomes: A potential male fertility influencer.

Andrologia 2021 Jul 2:e14155. Epub 2021 Jul 2.

Theriogenology Laboratory, Southern Regional Station of ICAR-National Dairy Research Institute, Bengaluru, India.

During transit and storage in epididymis, spermatozoa undergo final maturation, acquire motility, functional competence and the ability to fertilise an oocyte. Epididymal secretions contain a complex biochemical milieu of diverse inorganic ions, proteins, metabolites and other molecules. Since it is believed that spermatozoa are translationally silent, proteins appearing in them are thought to be synthesised elsewhere, including epididymis, and then incorporated to the cells. Read More

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