19,687 results match your criteria epidermal keratinocytes

Protective effects of sunscreen (50+) and octatrienoic acid 0.1% in actinic keratosis and UV damages.

J Investig Med 2021 Sep 16. Epub 2021 Sep 16.

Human Microbiome Advanced Project, Milan, Italy.

Actinic keratosis is a form of dysplastic epidermal lesion resulting from chronic and excessive UV exposure with a certain risk of becoming cancerous. Current guidelines advocated the use of sunscreens to prevent photodamage. An efficient photoprotection must involve both primary protective factors such as UV filters and secondary factors (eg, antioxidants) able to disrupt the photochemical and genetic cascade triggered by UVs. Read More

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September 2021

Impact of isoflavone genistein on psoriasis in in vivo and in vitro investigations.

Sci Rep 2021 Sep 14;11(1):18297. Epub 2021 Sep 14.

Department of Medical Biology and Genetics, University of Gdańsk, Wita Stwosza 59, 80-308, Gdańsk, Poland.

Genistein is applied worldwide as an alternative medicament for psoriasis (Ps) because of its anti-inflammatory activity and perceived beneficial impact on the skin. Hereby, we report our in vivo and in vitro investigations to supplement scientific research in this area. The reduction of clinical and biochemical scores in mild to moderate Ps patients taking genistein, its safety, good tolerability with no serious adverse events or discontinuations of treatment, no dose-limiting toxicities, negligible changes in pharmacodynamic parameters and remarkable serum interleukin level alterations were documented in this study. Read More

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September 2021

House dust mite-derived serine protease upregulates gene expression of interleukin-33 in canine keratinocytes via protease-activated receptor-2.

Vet Dermatol 2021 Sep 14. Epub 2021 Sep 14.

Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo, 183-8509, Japan.

Background: The involvement of interleukin (IL)-33 produced by keratinocytes has been suggested in the pathogenesis of canine atopic dermatitis (cAD). House dust mite (HDM)-derived proteases induce the production of various cytokines and chemokines in keratinocytes via protease-activated receptor-2 (PAR-2); however, their effects on IL-33 mRNA expression in canine keratinocytes have not been determined.

Hypothesis/objective: To clarify whether HDM-derived proteases induce IL-33 mRNA expression in canine keratinocytes via PAR-2. Read More

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September 2021

The Multitasking Surface Protein of Staphylococcus epidermidis: Accumulation-Associated Protein (Aap).

mBio 2021 Sep 14:e0198921. Epub 2021 Sep 14.

Microbial Genetics, Interfaculty Institute of Microbiology and Infection Medicine Tübingen (IMIT), University of Tübingen, Tübingen, Germany.

The stratum corneum is the outermost layer of the epidermis and is thus directly exposed to the environment. It consists mainly of corneocytes, which are keratinocytes in the last stage of differentiation, having neither nuclei nor organelles. However, they retain keratin filaments embedded in filaggrin matrix and possess a lipid envelope which protects the body from desiccation. Read More

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September 2021

Protective effects of GLHP from Gracilaria lemaneiformis against UVB-induced photodamage in human immortalized keratinocytes cells and BALB/c mice.

Exp Gerontol 2021 Sep 10:111550. Epub 2021 Sep 10.

Guangdong (Zhanjiang) Provincial Laboratory of Southern Marine Science and Engineering, 524023, PR China; Marine Biomedical Research Institution, Guangdong Medical University, Zhanjiang 524023, PR China; Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical University, Zhanjiang 524023, PR China. Electronic address:

Our previous study showed that the water-soluble heteropolysaccharide extracted from Gracilaria lemaneiformis (GLHP) has excellent anti-inflammation and anti-oxidant properties. This study explored the efficacy of GLHP against skin anti-photoaging in human immortalized keratinocytes (HaCaT) cells and BALB/c mice under UVB irradiation. Cell viability, antiapoptotic, reactive oxygen species (ROS) scavenging activity, mitochondrial membrane potential, and cell wound scratch assays were conducted, as well as assessment of inflammation markers and sun protection factors. Read More

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September 2021

Phenol-soluble modulins α are major virulence factors of secretome promoting inflammatory response in human epidermis.

Virulence 2021 12;12(1):2474-2492

Laboratoire Inflammation Tissus Epithéliaux et Cytokines EA 4331, Université De Poitiers, Poitiers, France.

is a skin commensal microorganism commonly colonizing healthy humans. Nevertheless, can also be responsible for cutaneous infections and contribute to flare-up of inflammatory skin diseases such as atopic dermatitis (AD), which is characterized by dysbiosis of the skin microbiota with as the predominant species. However, the role of major virulence factors of this pathogen such as phenol-soluble modulin (PSM) toxins in epidermal inflammation remains poorly understood. Read More

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December 2021

Deconstructing progressive inflammatory fibrosis in recessive dystrophic epidermolysis bullosa.

Christen L Ebens

EMBO Mol Med 2021 Sep 13:e14864. Epub 2021 Sep 13.

Pediatric Blood and Marrow Transplantation & Cellular Therapies, University of Minnesota, Minneapolis, MN, USA.

Recessive dystrophic epidermolysis bullosa (RDEB) is an inherited blistering skin disease, resulting from biallelic mutations in COL7A1, the gene encoding type VII collagen (C7). At mucocutaneous barriers, tissue integrity relies upon linked extracellular matrix (ECM) proteins forming a physiologic suture, connecting basal epidermal keratinocytes to the underlying dermis. C7 secreted from epidermal keratinocytes and dermal fibroblasts homotrimerizes in the upper dermis to form anchoring fibrils, a critical component of this suture. Read More

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September 2021

Overexpression of Lin28a Aggravates Psoriasis-Like Phenotype by Regulating the Proliferation and Differentiation of Keratinocytes.

J Inflamm Res 2021 30;14:4299-4312. Epub 2021 Aug 30.

School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National University, Daegu, Korea.

Purpose: Psoriasis is a common and well-studied autoimmune skin disease, which is characterized by plaques. The formation of psoriasis plaques occurs through the hyperproliferation and abnormal differentiation of keratinocytes, infiltration of numerous immune cells into the dermis, increased subepidermal angiogenesis, and various autoimmune-associated cytokines and chemokines. According to previous research, Lin28 regulates the let-7 family, and let-7b is associated with psoriasis. Read More

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Molecular origin of the effects of mutation on the structure and mechanical properties of human epithelial keratin K5/K14.

J Mech Behav Biomed Mater 2021 Sep 2;124:104798. Epub 2021 Sep 2.

Institute of Applied Mechanics, National Taiwan University, Taipei City, Taiwan. Electronic address:

Epithelial keratin, a type of intermediate filament (IF) protein, is one of the key components in maintaining the stability of the cell nucleus in the epidermis of the skin, the largest organ in the human body. It absorbs water and withstands external pressure, affecting the structural stability and mechanical properties of the skin. Epidermolysis bullosa simplex (EBS) is a rare genetic skin disease related to genetic mutations in epithelial keratin K5/K14. Read More

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September 2021

Syndecan-1 shedding by meprin β impairs keratinocyte adhesion and differentiation in hyperkeratosis.

Matrix Biol 2021 Sep 8. Epub 2021 Sep 8.

Biochemical Institute, Christian-Albrechts-University of Kiel, 24118 Kiel, Germany. Electronic address:

Dysregulation of proteolytic enzymes has huge impact on epidermal homeostasis, which can result in severe pathological conditions such as fibrosis or Netherton syndrome. The metalloprotease meprin β was found to be upregulated in hyperproliferative skin diseases. AP-1 transcription factor complex has been reported to induce Mep1b expression. Read More

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September 2021

Hemicentin-1 is an essential extracellular matrix component of the dermal-epidermal and myotendinous junctions.

Sci Rep 2021 Sep 9;11(1):17926. Epub 2021 Sep 9.

Institute of Zoology, Developmental Biology Unit, University of Cologne, Cologne, Germany.

The extracellular matrix architecture is composed of supramolecular fibrillar networks that define tissue specific cellular microenvironments. Hemicentins (Hmcn1 and Hmcn2) are ancient and very large members (> 600 kDa) of the fibulin family, whose short members are known to guide proper morphology and functional behavior of specialized cell types predominantly in elastic tissues. However, the tissue distribution and function of Hemicentins within the cellular microenvironment of connective tissues has remained largely unknown. Read More

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September 2021

Chronic UV radiation-induced RORγt+ IL-22-producing lymphoid cells are associated with mutant KC clonal expansion.

Proc Natl Acad Sci U S A 2021 Sep;118(37)

Department of Dermatology, Yale School of Medicine, New Haven, CT 06510

Chronic ultraviolet (UV) radiation exposure is the greatest risk factor for cutaneous squamous cell carcinoma (cSCC) development, and compromised immunity accelerates this risk. Having previously identified that epidermal Langerhans cells (LC) facilitate the expansion of UV-induced mutant keratinocytes (KC), we sought to more fully elucidate the immune pathways critical to cutaneous carcinogenesis and to identify potential targets of intervention. Herein, we reveal that chronic UV induces and LC enhance a local immune shift toward RORγt+ interleukin (IL)-22/IL-17A-producing cells that occurs in the presence or absence of T cells while identifying a distinct RORγt+ Sca-1+ CD103+ ICOS+ CD2 CCR6+ intracellular CD3+ cutaneous innate lymphoid cell type-3 (ILC3) population (uvILC3) that is associated with UV-induced mutant KC growth. Read More

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September 2021

The Characterization of Scaffolds Based on Dialdehyde Chitosan/Hyaluronic Acid.

Materials (Basel) 2021 Sep 1;14(17). Epub 2021 Sep 1.

Department of Biomaterials and Cosmetics Chemistry, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100 Toruń, Poland.

In this work, two-component dialdehyde chitosan/hyaluronic acid scaffolds were developed and characterized. Dialdehyde chitosan was obtained by one-step synthesis with chitosan and sodium periodate. Three-dimensional scaffolds were prepared by the lyophilization method. Read More

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September 2021

Longitudinal peripheral tissue RNA-Seq transcriptomic profiling, hyperalgesia, and wound healing in the rat plantar surgical incision model.

FASEB J 2021 Oct;35(10):e21852

Department of Perioperative Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA.

Postoperative pain and delayed healing in surgical wounds, which require complex management strategies have understudied complicated mechanisms. Here we investigated temporal changes in behavior, tissue structure, and transcriptomic profiles in a rat model of a surgical incision, using hyperalgesic behavioral tests, histological analyses, and next-generation RNA sequencing, respectively. The most rapidly (1 hour) expressed genes were the chemokines, Cxcl1 and Cxcl2. Read More

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October 2021

Apalutamide-associated skin rash in patients with prostate cancer: Histological evaluation by skin biopsy.

IJU Case Rep 2021 Sep 27;4(5):299-302. Epub 2021 Jun 27.

Department of Urology Faculty of Medicine Kagawa University Kagawa Japan.

Introduction: Apalutamide-associated skin rash is a more common adverse event in the Japanese population than in the global population. However, its mechanism remains elusive, and limited histopathological information hampers further understanding.

Case Presentation: Case 1: a 71-year-old man with metastatic castration-sensitive prostate cancer developed a skin rash after 70 days of apalutamide treatment. Read More

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September 2021

GRHL3 activates FSCN1 to relax cell-cell adhesions between migrating keratinocytes during wound reepithelialization.

JCI Insight 2021 Sep 8;6(17). Epub 2021 Sep 8.

Department of Biological Chemistry, School of Medicine.

The migrating keratinocyte wound front is required for skin wound closure. Despite significant advances in wound healing research, we do not fully understand the molecular mechanisms that orchestrate collective keratinocyte migration. Here, we show that, in the wound front, the epidermal transcription factor Grainyhead like-3 (GRHL3) mediates decreased expression of the adherens junction protein E-cadherin; this results in relaxed adhesions between suprabasal keratinocytes, thus promoting collective cell migration and wound closure. Read More

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September 2021

Developmental genetics of color pattern establishment in cats.

Nat Commun 2021 09 7;12(1):5127. Epub 2021 Sep 7.

HudsonAlpha Institute for Biotechnology, Huntsville, AL, USA.

Intricate color patterns are a defining aspect of morphological diversity in the Felidae. We applied morphological and single-cell gene expression analysis to fetal skin of domestic cats to identify when, where, and how, during fetal development, felid color patterns are established. Early in development, we identify stripe-like alterations in epidermal thickness preceded by a gene expression pre-pattern. Read More

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September 2021

Phospholipase A2 enzymes represent a shared pathogenic pathway in psoriasis and pityriasis rubra pilaris.

JCI Insight 2021 Sep 7. Epub 2021 Sep 7.

Department of Dermatology, University of Michigan, Ann Arbor, United States of America.

Altered epidermal differentiation along with increased keratinocyte proliferation, is a characteristic feature of psoriasis and pityriasis rubra pilaris (PRP). However, despite this large degree of overlapping clinical and histologic features, the molecular signatures these skin disorders share are unknown. Using global transcriptomic profiling we demonstrate that plaque psoriasis and PRP skin lesions have high overlap, with all differentially expressed genes in PRP relative to normal skin having complete overlap with those in psoriasis. Read More

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September 2021

Case Report: Positive Outcome of a Suspected Drug-Associated (Immune Mediated) Reaction in a 4-Year-Old Male French Bulldog.

Front Vet Sci 2021 20;8:728901. Epub 2021 Aug 20.

Small Animal Clinic, Université de Toulouse, ENVT, Toulouse, France.

Toxic epidermal necrolysis (TEN) is a rare and severe life-threatening syndrome characterized by apoptosis of keratinocytes resulting in devitalization of the epidermis affecting more than 30% of skin surface. In humans and animals, this condition is mostly triggered by drugs. Identification of the putative agent and its withdrawal are crucial to successful management of a patient with TEN. Read More

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Epicutaneous challenge with protease allergen requires its protease activity to recall T2 and T17/T22 responses in mice pre-sensitized via distant skin.

J Immunotoxicol 2021 12;18(1):118-126

Safety Science Research Laboratories, Kao Corporation, Tochigi, Japan.

Epicutaneous exposure to allergenic proteins is an important sensitization route for skin diseases like protein contact dermatitis, immunologic contact urticaria, and atopic dermatitis. Environmental allergen sources such as house dust mites contain proteases, which are frequent allergens themselves. Here, the dependency of T-helper (T) cell recall responses on allergen protease activity in the elicitation phase in mice pre-sensitized via distant skin was investigated. Read More

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December 2021

Concise Review: Considering Optimal Temperature for Short-Term Storage of Epithelial Cells.

Front Med (Lausanne) 2021 18;8:686774. Epub 2021 Aug 18.

Department of Ophthalmology, Oslo University Hospital, Oslo, Norway.

Transplantation of novel tissue-engineered products using cultured epithelial cells is gaining significant interest. While such treatments can readily be provided at centralized medical centers, delivery to patients at geographically remote locations requires the establishment of suitable storage protocols. One important aspect of storage technology is temperature. Read More

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Salicylic acid in ginseng root alleviates skin hyperpigmentation disorders by inhibiting melanogenesis and melanosome transport.

Eur J Pharmacol 2021 Sep 1;910:174458. Epub 2021 Sep 1.

Research Center of Traditional Chinese Medicine, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, 130021, China. Electronic address:

Abnormal melanogenesis and melanosome transport can cause skin pigmentation disorders that are often treated using ginseng-based formulation. We previously found that phenolic acid compounds in ginseng root could inhibit melanin production and as a skin-whitening agents. However, mechanisms of action underlying effects of ginseng phenolic acid monomers on melanogenesis remain unclear. Read More

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September 2021

Review. Regeneration of the tail in lizards appears regulated by a balanced expression of oncogenes and tumor suppressors.

Lorenzo Alibardi

Ann Anat 2021 Aug 31:151824. Epub 2021 Aug 31.

Comparative Histolab Padova and Dipartmento di Biologia, Universita' di Bologna, Italy. Electronic address:

Background: Tail regeneration in lizards is the only case of large multi-tissue organ regeneration in amniotes.

Methods: The present Review summarizes numerous immunolocalization and gene-expression studies indicating that after tail amputation in lizards the stump is covered in 7-10 days by the migration of keratinocytes. This allows the accumulation of mesenchymal-fibroblasts underneath the wound epidermis and forms a regenerative blastema and a new tail. Read More

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Fibroblast-derived matrices-based human skin equivalent as an psoriatic model for drug testing.

J Biosci 2021 ;46

School of Biosciences, Taylor's University, No. 1, Jalan Taylor's 47500 Subang Jaya, Selangor, Malaysia.

Psoriasis is a chronic skin disease characterized by thickening and disorganization of the skin's protective barrier. Although current models replicate some aspects of the disease, development of therapeutic strategies have been hindered by absence of more relevant models. This study aimed to develop and characterize an psoriatic human skin equivalent (HSE) using human keratinocytes HaCat cell line grown on fibroblasts-derived matrices (FDM). Read More

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January 2021

Hyaluronic acid-fibrin hydrogels show improved mechanical stability in dermo-epidermal skin substitutes.

Mater Sci Eng C Mater Biol Appl 2021 Sep 31;128:112352. Epub 2021 Jul 31.

Department of Bioengineering and Aerospace Engineering, Universidad Carlos III de Madrid (UC3M), Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain. Electronic address:

Human plasma-derived bilayered skin substitutes have been successfully used by our group in different skin tissue engineering applications. However, several issues associated with their poor mechanical properties were observed, and they often resulted in rapid contraction and degradation. In this sense, hydrogels composed of plasma-derived fibrin and thiolated-hyaluronic acid (HA-SH, 0. Read More

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September 2021

Arsenic leads to autophagy of keratinocytes by increasing aquaporin 3 expression.

Sci Rep 2021 Sep 1;11(1):17523. Epub 2021 Sep 1.

Department of Dermatology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.

Exposure to arsenic, a ubiquitous metalloid on Earth, results in human cancers. Skin cancer is the most common arsenical cancers. Both autophagy and aquaporin pathway are known to promote carcinogenesis. Read More

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September 2021

Alda-1, an Aldehyde Dehydrogenase 2 Agonist, Improves Cutaneous Wound Healing by Activating Epidermal Keratinocytes via Akt/GSK-3β/β-Catenin Pathway.

Aesthetic Plast Surg 2021 Aug 30. Epub 2021 Aug 30.

Department of Plastic and Reconstructive Surgery, Zhongshan Hospital Fudan University, 180 Fenglin Road, Xuhui District, Shanghai, 200032, People's Republic of China.

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Scanning electron microscopy of murine skin ultrathin sections and cultured keratinocytes.

STAR Protoc 2021 Sep 17;2(3):100729. Epub 2021 Aug 17.

A∗STAR Skin Research Lab (ASRL), Agency for Science, Technology and Research (A∗STAR) 8A Biomedical Grove, #6-11 Immunos, Singapore 138648, Singapore.

Generating high-quality electron microscopy images of the skin and keratinocytes can be challenging. Here we describe a simple protocol for scanning electron microscopy (SEM) of murine skin. The protocol enables characterization of the ultrastructure of the epidermis, dermis, hair follicles, basement membrane, and cell-cell junctions. Read More

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September 2021

Antiwrinkle efficacy of 1-ethyl-β-N-acetylglucosaminide, an inducer of epidermal hyaluronan production.

Skin Res Technol 2021 Aug 29. Epub 2021 Aug 29.

Biological Science Research, Kao Corporation, Kotobuki-cho, Odawara-shi, Kanagawa, Japan.

Background: Hyaluronan (HA) has a unique hydration capacity that contributes to firmness and bounciness of the skin. Epidermal HA declines with skin aging, which may lead to clinical signs of aging including skin wrinkles and loss of hydration and elasticity. Recently, we developed a new cosmetic agent 1-ethyl-β-N-acetylglucosaminide (β-NAG2), which enhances HA production in cultured human keratinocytes. Read More

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Interaction of galectin-7 with HMGCS1 in vitro may facilitate cholesterol deposition in cultured keratinocytes.

J Invest Dermatol 2021 Aug 26. Epub 2021 Aug 26.

Departments of Dermatology, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-8513, Japan.

Three-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase 1 (HMGCS1) was identified to interact with Gal-7, a pro-apoptotic β-galactoside binding protein, by yeast two-hybrid system. Their interaction was confirmed by in vitro β-galactosidase, BIA core and immunoprecipitation assays. Distinct interactive site of HMGCS1was found to reside at Phe-26. Read More

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