21,085 results match your criteria epidermal differentiation

CircAMD1 regulates proliferation and collagen synthesis via sponging miR-27a-3p in P63-mutant human dermal fibroblasts.

Differentiation 2021 Apr 30;119:10-18. Epub 2021 Apr 30.

Department of Pediatric Surgery, Shengjing Hospital of China Medical University, Shenyang, China.

Transcription factor p63 has critical functions in epidermal, hindgut/anorectal, and limb development. Human mutations in P63 correlate with congenital syndromes affecting the skin, anorectal, and limbs. Nevertheless, less are detected regarding networks and functions controlled by P63 mutations in dermal fibroblasts, which are closely related to skin physiology. Read More

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MMP-3 plays a major role in calcium pantothenate-promoted wound healing after fractional ablative laser treatment.

Lasers Med Sci 2021 May 14. Epub 2021 May 14.

Department of Dermatology and Allergology, Medical Faculty RWTH Aachen University, Pauwelsstrasse 30, 52074, Aachen, Germany.

Ablative fractional laser treatment leads to a loss of matrix metalloproteinase-3 (MMP-3) expression; therefore, in the present in vitro study, we addressed the role of MMP-3 and its regulation by calcium pantothenate in wound healing processes at the molecular level. Utilizing confocal laser microscopy, we investigated MMP-3 protein expression in fractional ablative CO laser-irradiated skin models. In addition, we established full-thickness 3D skin models using fibroblasts and keratinocytes with a MMP-3 knockdown that were irradiated with a fractional ablative Er:YAG laser to set superficial injuries with standardized dimensions and minimal thermal damage to the surrounding tissue. Read More

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2'-Hydroxycinnamaldehyde ameliorates imiquimod-induced psoriasiform inflammation by targeting PKM2-STAT3 signaling in mice.

Exp Mol Med 2021 May 14. Epub 2021 May 14.

Department of Biochemistry and Molecular Biology, Chonbuk National University Medical School, Jeonju, Jeonbuk, 54896, Republic of Korea.

2'-Hydroxycinnamaldehyde (HCA), the active component isolated from the stem bark of Cinnamomum cassia, exerts anticancer effects through multiple mechanisms. We recently determined that HCA inhibits signal transducer and activator of transcription 3 (STAT3) signaling in prostate cancer cells. Because STAT3 overactivation has been closely associated with the development of psoriasis, a chronic autoimmune skin disease, we examined whether HCA ameliorates skin lesions in an imiquimod-induced psoriasis-like mouse model. Read More

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CG6015 controls spermatogonia transit-amplifying divisions by epidermal growth factor receptor signaling in Drosophila testes.

Cell Death Dis 2021 May 14;12(5):491. Epub 2021 May 14.

Institute of Reproductive Medicine, School of Medicine, Nantong University, Nantong, China.

Spermatogonia transit-amplifying (TA) divisions are crucial for the differentiation of germline stem cell daughters. However, the underlying mechanism is largely unknown. In the present study, we demonstrated that CG6015 was essential for spermatogonia TA-divisions and elongated spermatozoon development in Drosophila melanogaster. Read More

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The endoribonuclease N4BP1 prevents psoriasis by controlling both keratinocytes proliferation and neutrophil infiltration.

Cell Death Dis 2021 May 14;12(5):488. Epub 2021 May 14.

Department of Pathogenic Biology, School of Medicine, Nantong University, 226001, Jiangsu, China.

Psoriasis is a common chronic skin disease, characterized by abnormal interplay between hyperproliferative epidermal keratinocytes and self-reactive immune cells with not fully addressed molecular mechanism. N4BP1 (NEDD4-binding protein 1) is considered as an immune regulator for a long time but its physiological role is not determined yet. Here, we found that the expression of N4BP1 in skin was highest among all 54 tested tissues, and its expression was further upregulated in psoriatic skin. Read More

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Metaplastic Breast Carcinoma: Clinicopathological Parameters and Prognostic Profile.

Cureus 2021 Apr 7;13(4):e14347. Epub 2021 Apr 7.

Pathology, Liaquat National Hospital and Medical College, Karachi, PAK.

Introduction Metaplastic breast carcinoma (MBC) is defined as breast cancer with a heterologous non-glandular component. MBC is considered a special type of breast cancer with a prognosis that is worse than invasive ductal carcinoma (IDC) of the breast. MBC is the most common breast cancer with a triple-negative profile. Read More

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The polyamine regulator AMD1 up-regulates spermine levels to drive epidermal differentiation.

J Invest Dermatol 2021 May 10. Epub 2021 May 10.

Maintaining tissue homeostasis depends on a balance of cell proliferation, differentiation and apoptosis. Within the epidermis the levels of the polyamines putrescine, spermidine and spermine are altered in many different skin conditions yet their role in epidermal tissue homeostasis is poorly understood. We identify the polyamine regulator, AMD1, as a crucial regulator of keratinocyte differentiation. Read More

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Ebosin Ameliorates Psoriasis-Like Inflammation of Mice miR-155 Targeting on IL-17 Pathway.

Front Immunol 2021 26;12:662362. Epub 2021 Apr 26.

NHC Key Laboratory of Biotechnology of Antibiotics, CAMS Key Laboratory of Synthetic Biology for Drug Innovation, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

Psoriasis is a recurrent autoimmune skin disease with aberrant regulation of keratinocytes and immunocytes. There is no universally accepted single treatment available for psoriasis, and the establishment of a common treatment option to control its signs and symptoms is urgently needed. Here, we found Ebosin, a novel exopolysaccharide isolated from sp. Read More

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Immune and barrier characterization of atopic dermatitis skin phenotype in Tanzanian patients.

Ann Allergy Asthma Immunol 2021 May 8. Epub 2021 May 8.

Laboratory of Inflammatory Skin Diseases, Department of Dermatology, Icahn School of Medicine at Mount Sinai, New York, New York, USA. Electronic address:

Background: Atopic dermatitis/AD is a common disease, with particularly high prevalence seen in Africa. It is increasingly recognized that AD patients of different ethnic backgrounds have unique molecular signatures in skin, potentially accounting for treatment response variations. However, the skin profile of AD patients from Africa is unknown, hindering development of new treatments targeted to this patient population. Read More

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Micromorphological differentiation of Korean Disporum species using light and scanning electron microscopy.

Microsc Res Tech 2021 May 10. Epub 2021 May 10.

Department of Biological Science, College of Bioscience and Biotechnology, Chungnam National University, Daejeon, Republic of Korea.

The genus Disporum Salisb. is widely distributed in East Asia, yet phylogenetically relevant morphological traits useful for differentiating many of the small, perennial, herbaceous species remain poorly described. To address this, leaf, floral, pollen, and orbicule micromorphology of four Korean Disporum species was investigated using light and scanning electron microscopy. Read More

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J Invest Dermatol 2021 May 6. Epub 2021 May 6.

Center for Biochemistry, Medical Faculty, University of Cologne, Cologne, Germany. Electronic address:

The skin epidermis is attached to the underlying dermis by a laminin 332-rich basement membrane. Consequently, loss of laminin 332 leads to the severe blistering disorder epidermolysis bullosa junctionalis in human and animals. Due to the indispensable role of laminin 332 in keratinocyte adhesion in vivo, the severity of the disease has limited research into other functions of the protein. Read More

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A role for the fusogen eff-1 in epidermal stem cell number robustness in Caenorhabditis elegans.

Sci Rep 2021 May 7;11(1):9787. Epub 2021 May 7.

Department of Life Sciences, Imperial College, London, SW7 2AZ, UK.

Developmental patterning in Caenorhabditis elegans is known to proceed in a highly stereotypical manner, which raises the question of how developmental robustness is achieved despite the inevitable stochastic noise. We focus here on a population of epidermal cells, the seam cells, which show stem cell-like behaviour and divide symmetrically and asymmetrically over post-embryonic development to generate epidermal and neuronal tissues. We have conducted a mutagenesis screen to identify mutants that introduce phenotypic variability in the normally invariant seam cell population. Read More

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Selective sweep for an enhancer involucrin allele identifies skin barrier adaptation out of Africa.

Nat Commun 2021 05 7;12(1):2557. Epub 2021 May 7.

Division of Dermatology, Department of Medicine, Washington University in St. Louis School of Medicine, St. Louis, MO, 63110, USA.

The genetic modules that contribute to human evolution are poorly understood. Here we investigate positive selection in the Epidermal Differentiation Complex locus for skin barrier adaptation in diverse HapMap human populations (CEU, JPT/CHB, and YRI). Using Composite of Multiple Signals and iSAFE, we identify selective sweeps for LCE1A-SMCP and involucrin (IVL) haplotypes associated with human migration out-of-Africa, reaching near fixation in European populations. Read More

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Distinct identities of leaf phloem cells revealed by single cell transcriptomics.

Plant Cell 2021 May;33(3):511-530

Institute for Molecular Physiology and Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich-Heine-University Düsseldorf, Düsseldorf 40225, Germany.

The leaf vasculature plays a key role in solute translocation. Veins consist of at least seven distinct cell types, with specific roles in transport, metabolism, and signaling. Little is known about leaf vascular cells, in particular the phloem parenchyma (PP). Read More

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[Roles of growth factors on vascular remodeling in pulmonary hypertension].

Nihon Yakurigaku Zasshi 2021 ;156(3):161-165

Department of Physiology, Aichi Medical University.

Pulmonary hypertension (PH) is defined as mean pulmonary arterial pressure at rest ≥25 mmHg. Pulmonary arterial hypertension (PAH) is classified as group 1 of PH and is a progressive and fatal disease of the pulmonary artery. The pathogenesis is sustained pulmonary vasoconstriction and pulmonary vascular remodeling, which cause progressive elevations in pulmonary vascular resistance and pulmonary arterial pressure. Read More

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Direct visualization of epithelial microvilli biogenesis.

Curr Biol 2021 Apr 28. Epub 2021 Apr 28.

Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN 37232, USA. Electronic address:

Microvilli are actin-bundle-supported surface protrusions that play essential roles in diverse epithelial functions. To develop our understanding of microvilli biogenesis, we used live imaging to directly visualize protrusion growth at early stages of epithelial differentiation. Time-lapse data revealed that specific factors, including epidermal growth factor pathway substrate 8 (EPS8) and insulin-receptor tyrosine kinase substrate (IRTKS) (also known as BAIAP2L1), appear in diffraction-limited puncta at the cell surface and mark future sites of microvillus growth. Read More

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Surface-enhanced Raman spectroscopic chemical imaging reveals distribution of pectin and its co-localization with xyloglucan inside onion epidermal cell wall.

PLoS One 2021 5;16(5):e0250650. Epub 2021 May 5.

Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, IA, United States of America.

The primary plant cell wall is a complex matrix composed of interconnected polysaccharides including cellulose, hemicellulose, and pectin. Changes of this dynamic polysaccharide system play a critical role during plant cell development and differentiation. A better understanding of cell wall architectures can provide insight into the plant cell development. Read More

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Biomolecular modifications during keratinocyte differentiation: Raman spectroscopy and chromatographic techniques.

Analyst 2021 May;146(9):2965-2973

Lipides: systèmes analytiques et biologiques, Université Paris-Saclay, 92296 Châtenay-Malabry, France.

From the basal layer until the stratum corneum, lipid and protein biomarkers associated with morphological changes denote keratinocyte differentiation and characterize each epidermis layer. Herein, we followed keratinocyte differentiation in the early stages using HaCaT cells over a period of two weeks by two complementary analytical techniques: Raman microspectroscopy and high-performance liquid chromatography coupled with high resolution mass spectrometry. A high concentration of calcium in the medium induced HaCaT cell differentiation in vitro. Read More

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Loss of DLX3 tumor suppressive function promotes progression of SCC through EGFR-ERBB2 pathway.

Oncogene 2021 May 4. Epub 2021 May 4.

Laboratory of Skin Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, NIH, Bethesda, MD, USA.

Cutaneous squamous cell carcinoma (cSCC) ranks second in the frequency of all skin cancers. The balance between keratinocyte proliferation and differentiation is disrupted in the pathological development of cSCC. DLX3 is a homeobox transcription factor which plays pivotal roles in embryonic development and epidermal homeostasis. Read More

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Effects of Flower Extract and Its Active Constituent Isoorientin on Skin Moisturization via Regulating Involucrin Expression: In Vitro and Molecular Docking Studies.

Molecules 2021 Apr 30;26(9). Epub 2021 Apr 30.

College of Pharmacy, Gachon University, 191 Hambakmoero, Yeonsu-gu, Incheon 21936, Korea.

Skin moisturization is very crucial for maintaining the flexibility, viscoelasticity, and differentiation of the epidermis and its deprivation causes several diseases from dry skin to dermatitis. , a miracle plant having diverse medicinal properties including skin moisturization effects. This study investigated for the first time the molecular mechanism targeting skin moisturization effects of the flower and its major active constituent. Read More

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The Act of Controlling Adult Stem Cell Dynamics: Insights from Animal Models.

Biomolecules 2021 Apr 30;11(5). Epub 2021 Apr 30.

Regional Centre for Biotechnology, NCR Biotech Science Cluster, 3rd Milestone, Gurgaon-Faridabad Ex-pressway, Faridabad 121001, India.

Adult stem cells (ASCs) are the undifferentiated cells that possess self-renewal and differentiation abilities. They are present in all major organ systems of the body and are uniquely reserved there during development for tissue maintenance during homeostasis, injury, and infection. They do so by promptly modulating the dynamics of proliferation, differentiation, survival, and migration. Read More

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A tale of two morphs: developmental patterns and mechanisms of seed coat differentiation in the dimorphic diaspore model Aethionema arabicum (Brassicaceae).

Plant J 2021 May 4. Epub 2021 May 4.

Department of Biological Sciences, Royal Holloway University of London, Egham, TW20 0EX, United Kingdom.

The developmental transition from a fertilised ovule to a dispersed diaspore (seed or fruit) involves complex differentiation processes of the ovule's integuments leading to the diversity in mature seed coat structures in angiosperms. In this study, comparative imaging and transcriptome analysis were combined to investigate the morph-specific developmental differences during outer seed coat differentiation and mucilage production in Aethionema arabicum, the Brassicaceae model for diaspore dimorphism. One of the intriguing adaptations of this species is the production and dispersal of morphologically distinct, mucilaginous and non-mucilaginous diaspores from the same plant (dimorphism). Read More

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Mechanical stimulation induces rapid fibroblast proliferation and accelerates the early maturation of human skin substitutes.

Biomaterials 2021 Jun 27;273:120779. Epub 2021 Mar 27.

Institute for Mechanical Systems, Department of Mechanical and Process Engineering, ETH Zurich, Leonhardstrasse 21, 8092 Zurich, Switzerland; Empa, Swiss Federal Laboratories for Materials Science and Technology, Überlandstrasse 129, 8600 Dübendorf, Switzerland. Electronic address:

The clinical treatment of large, full-thickness skin injuries with tissue-engineered autologous dermo-epidermal skin substitutes is an emerging alternative to split-thickness skin grafting. However, their production requires about one month of in vitro cell and tissue culture, which is a significant drawback for the treatment of patients with severe skin defects. With the aim to reduce the production time, we developed a new dynamic bioreactor setup that applies cyclic biaxial tension to collagen hydrogels for skin tissue engineering. Read More

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LCM and RNA-seq analyses revealed roles of cell cycle and translational regulation and homoeolog expression bias in cotton fiber cell initiation.

BMC Genomics 2021 Apr 29;22(1):309. Epub 2021 Apr 29.

Department of Molecular Biosciences, and Center for Computational Biology and Bioinformatics, The University of Texas at Austin, Austin, TX, 78712, USA.

Background: Cotton fibers provide a powerful model for studying cell differentiation and elongation. Each cotton fiber is a singular and elongated cell derived from epidermal-layer cells of a cotton seed. Efforts to understand this dramatic developmental shift have been impeded by the difficulty of separation between fiber and epidermal cells. Read More

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Diversified Stimuli-Induced Inflammatory Pathways Cause Skin Pigmentation.

Int J Mol Sci 2021 Apr 12;22(8). Epub 2021 Apr 12.

Department of Dermatology, Faculty of Medicine, Jichi Medical University, Tochigi 329-0498, Japan.

The production of melanin pigments by melanocytes and their quantity, quality, and distribution play a decisive role in determining human skin, eye, and hair color, and protect the skin from adverse effects of ultraviolet radiation (UVR) and oxidative stress from various environmental pollutants. Melanocytes reside in the basal layer of the interfollicular epidermis and are compensated by melanocyte stem cells in the follicular bulge area. Various stimuli such as eczema, microbial infection, ultraviolet light exposure, mechanical injury, and aging provoke skin inflammation. Read More

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Structures of the ß-Keratin Filaments and Keratin Intermediate Filaments in the Epidermal Appendages of Birds and Reptiles (Sauropsids).

David A D Parry

Genes (Basel) 2021 Apr 17;12(4). Epub 2021 Apr 17.

School of Fundamental Sciences, Massey University, Private Bag 11-222, Palmerston North 4442, New Zealand.

The epidermal appendages of birds and reptiles (the sauropsids) include claws, scales, and feathers. Each has specialized physical properties that facilitate movement, thermal insulation, defence mechanisms, and/or the catching of prey. The mechanical attributes of each of these appendages originate from its fibril-matrix texture, where the two filamentous structures present, i. Read More

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Differential and Overlapping Effects of Melatonin and Its Metabolites on Keratinocyte Function: Bioinformatics and Metabolic Analyses.

Antioxidants (Basel) 2021 Apr 17;10(4). Epub 2021 Apr 17.

Department of Dermatology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.

We investigated the effects of melatonin and its selected metabolites, i.e., -Acetyl--formyl-5-methoxykynurenamine (AFMK) and 6-hydroxymelatonin (6(OH)Mel), on cultured human epidermal keratinocytes (HEKs) to assess their homeostatic activities with potential therapeutic implications. Read More

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The Role of Bifidobacteria in Predictive and Preventive Medicine: A Focus on Eczema and Hypercholesterolemia.

Microorganisms 2021 Apr 14;9(4). Epub 2021 Apr 14.

Fidia Farmaceutici S.p.A., R&D Unità locale Fidia Research sud, Contrada Pizzuta, 96017 Noto, Italy.

Bifidobacteria colonize the human gastrointestinal tract early on in life, their interaction with the host starting soon after birth. The health benefits are strain specific and could be due to the produced polysaccharides. The consumption of probiotics may prevent obesity, irritable bowel syndrome, eczema or atopic dermatitis, and asthma. Read More

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Simultaneous Quantitative Analysis of Ginsenosides Isolated from the Fruit of C.A. Meyer and Regulation of HO-1 Expression through EGFR Signaling Has Anti-Inflammatory and Osteogenic Induction Effects in HPDL Cells.

Molecules 2021 Apr 6;26(7). Epub 2021 Apr 6.

College of Pharmacy, Keimyung University, 1095 Dalgubeol-daero, Daegu 42601, Korea.

Periodontitis is a set of chronic inflammatory diseases caused by the accumulation of Gram-negative bacteria on teeth, resulting in gingivitis, pocket formation, alveolar bone loss, tissue destruction, and tooth loss. In this study, the contents of ginsenosides isolated from fruit extract were quantitatively analyzed, and the anti-inflammatory effects were evaluated in human periodontal ligament cells. The major ginsenosides, Re, Ra8, and Rf, present in ginseng fruit were simultaneously analyzed by a validated method using high-performance liquid chromatography with a diode-array detector; Re, Ra8, and Rf content per 1 g of fruit extract was 1. Read More

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