6,565 results match your criteria epidermal barrier

RIPK1 regulates cell function and death mediated by UVB radiation and TNF-α.

Mol Immunol 2021 May 5;135:304-311. Epub 2021 May 5.

College of Pharmaceutical Sciences, Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Institute of Life Science and Green Development, Hebei University, Baoding, 071002, China. Electronic address:

The RIP family plays a key role in mediating cell inflammation, oxidative stress and death. Among them, RIPK1, as an important regulatory factor in the upstream of the NF-κB pathway, is involved in multiple pathways of cell inflammation and death. Epidermal cells constitute the outermost barrier of the human body. Read More

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Selective sweep for an enhancer involucrin allele identifies skin barrier adaptation out of Africa.

Nat Commun 2021 May 7;12(1):2557. Epub 2021 May 7.

Division of Dermatology, Department of Medicine, Washington University in St. Louis School of Medicine, St. Louis, MO, 63110, USA.

The genetic modules that contribute to human evolution are poorly understood. Here we investigate positive selection in the Epidermal Differentiation Complex locus for skin barrier adaptation in diverse HapMap human populations (CEU, JPT/CHB, and YRI). Using Composite of Multiple Signals and iSAFE, we identify selective sweeps for LCE1A-SMCP and involucrin (IVL) haplotypes associated with human migration out-of-Africa, reaching near fixation in European populations. Read More

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Topical emollient for preventing infection in preterm infants.

Cochrane Database Syst Rev 2021 May 7;5:CD001150. Epub 2021 May 7.

Centre for Reviews and Dissemination, University of York, York, UK.

Background: Breakdown of the developmentally immature epidermal barrier may permit entry for micro-organisms leading to invasive infection in preterm infants. Topical emollients may improve skin integrity and barrier function and thereby prevent invasive infection, a major cause of mortality and morbidity in preterm infants.

Objectives: To assess the effect of topical application of emollients (ointments, creams, or oils) on the risk of invasive infection and mortality in preterm infants. Read More

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Mesenchymal stem cells derived from human dental follicle modulate the aberrant immune response in atopic dermatitis.

Immunotherapy 2021 May 6. Epub 2021 May 6.

Division of Pediatric Allergy & Immunology, Marmara University, Istanbul, Turkey.

Atopic dermatitis (AD) is an inflammatory cutaneous disorder. The advancements in the understanding of AD immunological pathogenesis have caused the development of therapies that suppress the dysregulated immune response. We aimed to evaluate the immunomodulatory effect of dental stem cells (dental follicle-mesenchymal stem cells [DF-MSCs]) on AD patients. Read More

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Comprehensive Mouse Skin Ceramide Analysis on a Solid-Phase and TLC Separation with High-Resolution Mass Spectrometry Platform.

Methods Mol Biol 2021 ;2306:139-155

Mass Spectrometry Resource, Division of Endocrinology, Metabolism, and Lipid Research, Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA.

Lipidomic analyses by mass spectrometry (MS) of epidermal ceramides, a large family of lipids crucial to the permeability barrier of the skin, have been reported previously. To ensure the accuracy of lipid identification, we describe here the isolation of mouse newborn epidermal lipids followed by fractionation with solid-phase extraction columns, and lipidomic analyses by high-resolution MS for structural identification. We also describe here the employment of thin layer chromatography, an old but useful tool, in facilitating the structural characterization of the epidermal lipid species by MS. Read More

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January 2021

Topical Applications of a Novel Emollient Inhibit Inflammation in Murine Models of Acute Contact Dermatitis.

Biomed Res Int 2021 13;2021:5594646. Epub 2021 Apr 13.

Dermatology Hospital of Southern Medical University, Guangdong 510091, China.

The benefits of emollients for eczematous dermatitis and psoriasis have been thought to be due to the improvements in epidermal function, including epidermal permeability barrier, stratum corneum hydration, and stratum corneum pH. We determined here whether emollient can direct inhibit cutaneous inflammation. Ear inflammation was induced by topical application of either 12-O-tetradecanoylphorbol-13-acetate (TPA) or 1-fluoro-2,4-dinitrofluorobenzene (DNFB). Read More

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Effects of Flower Extract and Its Active Constituent Isoorientin on Skin Moisturization via Regulating Involucrin Expression: In Vitro and Molecular Docking Studies.

Molecules 2021 Apr 30;26(9). Epub 2021 Apr 30.

College of Pharmacy, Gachon University, 191 Hambakmoero, Yeonsu-gu, Incheon 21936, Korea.

Skin moisturization is very crucial for maintaining the flexibility, viscoelasticity, and differentiation of the epidermis and its deprivation causes several diseases from dry skin to dermatitis. , a miracle plant having diverse medicinal properties including skin moisturization effects. This study investigated for the first time the molecular mechanism targeting skin moisturization effects of the flower and its major active constituent. Read More

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Nod-Like Receptors in Host Defence and Disease at the Epidermal Barrier.

Int J Mol Sci 2021 Apr 28;22(9). Epub 2021 Apr 28.

Department of Dermatology, University Hospital Zürich (USZ), University of Zürich (UZH), Raemistrasse 100, 8091 Zürich, Switzerland.

The nucleotide-binding domain and leucine-rich-repeat-containing family (NLRs) (sometimes called the NOD-like receptors, though the family contains few bona fide receptors) are a superfamily of multidomain-containing proteins that detect cellular stress and microbial infection. They constitute a critical arm of the innate immune response, though their functions are not restricted to pathogen recognition and members engage in controlling inflammasome activation, antigen-presentation, transcriptional regulation, cell death and also embryogenesis. NLRs are found from basal metazoans to plants, to zebrafish, mice and humans though functions of individual members can vary from species to species. Read More

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Molecular Mechanisms of Atopic Dermatitis Pathogenesis.

Int J Mol Sci 2021 Apr 16;22(8). Epub 2021 Apr 16.

Department of Dermatology, Venereology and Allergology, Medical University of Gdansk, 80-214 Gdańsk, Poland.

Atopic dermatitis is a chronic, non-infectious inflammatory dermatosis. Acharacteristic feature is persistent itching of the skin. The chronic, relapsing course of the disease, economic burden, and the whole family's involvement in the treatment process immensely reduce the quality of life of patients and their families. Read More

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Biologically Active Compounds in Inflorescences: Isolation, Phytochemical Characterization and Effects on the Skin Biophysical Parameters.

Int J Mol Sci 2021 Apr 23;22(9). Epub 2021 Apr 23.

Department and Division of Practical Cosmetology and Skin Diseases Prophylaxis, Poznan University of Medicinal Sciences, Mazowiecka 33, 60-623 Poznan, Poland.

Three germacranolides, as well as five flavonoids, natural steroid and simple phenolic compounds, were isolated from the inflorescence of growing in Iran. The paper presents active compounds found for the first time in the inflorescence of this species. The flavonoids, simple phenolic compounds and natural steroids have been isolated for the first time in the genus . Read More

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Effects of CJLP55 on Clinical Improvement, Skin Condition and Urine Bacterial Extracellular Vesicles in Patients with Acne Vulgaris: A Randomized, Double-Blind, Placebo-Controlled Study.

Nutrients 2021 Apr 19;13(4). Epub 2021 Apr 19.

Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University, Yongin-si 17104, Gyeongggi-do, Korea.

CJLP55 has anti-pathogenic bacterial and anti-inflammatory activities in vitro. We investigated the dietary effect of CJLP55 supplement in patients with acne vulgaris, a prevalent inflammatory skin condition. Subjects ingested CJLP55 or placebo ( = 14 per group) supplements for 12 weeks in this double-blind, placebo-controlled randomized study. Read More

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Detection of Antimicrobial Peptides in Stratum Corneum by Mass Spectrometry.

Int J Mol Sci 2021 Apr 19;22(8). Epub 2021 Apr 19.

Proteomics Core Facility, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary.

Antimicrobial and immunomodulatory peptides (AMPs) are considered as the key players in the maintenance of skin barrier functions. Here, we developed a novel approach for the examination of AMPs in the outermost layer of the epidermis, namely stratum corneum (SC). The SC sample collection by tape stripping was coupled with detection by highly specific and sensitive parallel reaction monitoring (PRM)-based mass spectrometry. Read More

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The Role of Bifidobacteria in Predictive and Preventive Medicine: A Focus on Eczema and Hypercholesterolemia.

Microorganisms 2021 Apr 14;9(4). Epub 2021 Apr 14.

Fidia Farmaceutici S.p.A., R&D Unità locale Fidia Research sud, Contrada Pizzuta, 96017 Noto, Italy.

Bifidobacteria colonize the human gastrointestinal tract early on in life, their interaction with the host starting soon after birth. The health benefits are strain specific and could be due to the produced polysaccharides. The consumption of probiotics may prevent obesity, irritable bowel syndrome, eczema or atopic dermatitis, and asthma. Read More

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Pathogenic and Compensatory Mechanisms in Epidermis of Sphingomyelin Synthase 2-Deficient Mice.

Skin Pharmacol Physiol 2021 Apr 29:1-7. Epub 2021 Apr 29.

Department of Dermatology, Faculty of Medicine, Saitama Medical University, Moroyama, Japan.

Sphingomyelin (SM) is a constituent of cellular membranes, while ceramides (Cer) produced from SM on plasma membranes serve as a lipid mediator that regulates cell proliferation, differentiation, and apoptosis. In the skin, SM also is a precursor of Cer, an important constituent of epidermal permeability barrier. We investigated the role of epidermal SM synthase (SMS)2, an isoform of SMS, which modulates SM and Cer levels on plasma membranes. Read More

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Vulvovaginal involvement in pediatric Stevens-Johnson syndrome: A case series.

J Pediatr Adolesc Gynecol 2021 Apr 26. Epub 2021 Apr 26.

Eunice Kennedy Shriver National Institute of Health and Human Development, National Institutes of Health.

Background: Stevens-Johnson Syndrome (SJS) and Toxic Epidermal Necrolysis (TEN) are life-threatening spectra of mucocutaneous delayed hypersensitivity reactions. Prodromal viral-like symptoms are followed by a characteristic diffuse rash caused by keratinocyte apoptosis and epidermal detachment.

Cases: Three adolescents were admitted with SJS/TEN and vulvovaginal involvement following initiation of Lamictal, Bactrim, and Phenobarbital. Read More

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Therapeutic New Era for Atopic Dermatitis: Part 1. Biologics.

Ann Dermatol 2021 Feb 30;33(1):1-10. Epub 2020 Dec 30.

Department of Dermatology, Oregon Health & Science University, Portland, OR, United States.

Atopic dermatitis (AD) is a chronic, inflammatory cutaneous disease driven by immune dysregulation and skin barrier dysfunction. We are currently experiencing a new era of understanding of the pathogenesis of AD and, as a consequence, a new era of innovation in therapeutics, including small molecules and biologic therapy. Recently, advances in translational research have challenged the traditional AD pathogenesis paradigm of AD being solely a Th2-dominant disease. Read More

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February 2021

Effects of Particulate Matter in a Mouse Model of Oxazolone-Induced Atopic Dermatitis.

Ann Dermatol 2020 Dec 11;32(6):496-507. Epub 2020 Nov 11.

Department of Dermatology, Chung-Ang University College of Medicine, Seoul, Korea.

Background: Recent epidemiological studies have demonstrated that air pollution is associated with the inflammatory response and may aggravate inflammatory skin diseases such as atopic dermatitis (AD). However, it is unclear whether particulate matter (PM) aggravates AD symptoms.

Objective: The aim of this study was to investigate whether PM exposure affects the skin barrier dysfunction and aggravates AD symptoms using human keratinocytes (HaCaT) cells and a mouse model of oxazolone-induced AD-like skin. Read More

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December 2020

A Randomized, Placebo-Controlled Trial of Gamma Linolenic Acid as an Add-on Therapy to Minocycline for the Treatment of Rosacea.

Ann Dermatol 2020 Dec 11;32(6):466-472. Epub 2020 Nov 11.

Department of Dermatology, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan, Korea.

Background: A recent study suggested a possible role of skin barrier dysfunction in the pathogenesis of rosacea, which leads to irritation symptoms. Gamma linolenic acid (GLA) is an essential omega-6 fatty acid that is known to restore defective epidermal skin barrier. GLA supplementation has not previously been performed in rosacea patients. Read More

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December 2020

Increased 1-Deoxysphingolipids and Skin Barrier Dysfunction in the Skin of X-ray or Ultraviolet B Irradiation and Atopic Dermatitis Lesion Could Be Prevented by Moisturizer with Physiological Lipid Mixture.

Ann Dermatol 2020 Aug 30;32(4):306-318. Epub 2020 Jun 30.

Department of Dermatology, Hallym University Kangnam Sacred Heart Hospital, Hallym University College of Medicine, Seoul, Korea.

Background: Skin diseases characterized by epithelial barrier dysfunction show altered sphingolipid metabolism, which results in changes in the stratum corneum intercellular lipid components and structure. Under pathological conditions, 1-deoxysphingolipids form as atypical sphingolipids from sphingolipid biosynthesis.

Objective: This study investigated the potential role of 1-deoxysphingolipids in skin barrier dysfunction secondary to X-ray and ultraviolet B (UVB) irradiation and . Read More

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Hyaluronan Oligosaccharides Improve Rosacea-Like Phenotype through Anti-Inflammatory and Epidermal Barrier-Improving Effects.

Ann Dermatol 2020 Jun 24;32(3):189-196. Epub 2020 Apr 24.

Department of Dermatology, CHA Bundang Medical Center, CHA University School of Medicine, Seongnam, Korea.

Background: Rosacea is a common skin disease associated with increased expression of cathelicidin, kallikrein 5 (KLK5), toll-like receptor (TLR) 2, and abnormal barrier function. Recently, it was reported that hyaluronan (HA) could influence immune function via various receptors and HA oligosaccharides (oligo-HAs) could suppress TLR-dependent cytokine expression.

Objective: We investigated if oligo-HAs could influence on inflammation and epidermal barrier induced by LL-37, which had a major role in rosacea. Read More

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Epigenetic Regulation of Filaggrin Gene Expression in Human Epidermal Keratinocytes.

Ann Dermatol 2020 Apr 11;32(2):122-129. Epub 2020 Mar 11.

Bio-Integration Research Center for Nutra-Pharmaceutical Epigenetics, Chung-Ang University, Seoul, Korea.

Background: Loss-of-function mutations in the filaggrin gene (FLG), which encodes an epidermal protein crucial for the formation of a functional skin barrier, have been identified as a major predisposing factor in the etiopathogenesis of atopic dermatitis (AD). Recent reports of relatively low frequencies of FLG-null mutations among specific ethnic groups with AD necessitated analysis of the epigenetic regulation which may control FLG expression without altering its DNA sequence.

Objective: The study aimed to identify DNA methylation-dependent regulation of FLG expression. Read More

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Exploratory Study of Epidermis, Basement Membrane Zone, Upper Dermis Alterations and Wnt Pathway Activation in Melasma Compared to Adjacent and Retroauricular Skin.

Ann Dermatol 2020 Apr 11;32(2):101-108. Epub 2020 Mar 11.

Department of Dermatology and Radioterapy, Botucatu School of Medicine, São Paulo State University, Botucatu, Brazil.

Background: Melasma is a chronic acquired focal hypermelanosis which pathogenesis has not been fully elucidated. Classical pathophysiologic studies have analysed the affected and perilesional areas, but little is known about the status of sun-protected skin, which is subjected to the same endogenous and genetic factors.

Objective: To assess the histological characteristics of melasma compared to adjacent and retroauricular skin. Read More

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How autophagy controls the intestinal epithelial barrier.

Autophagy 2021 Apr 27:1-18. Epub 2021 Apr 27.

Department of Immunology, University of Toronto, Toronto, Canada.

Macroautophagy/autophagy is a cellular catabolic process that results in lysosome-mediated recycling of organelles and protein aggregates, as well as the destruction of intracellular pathogens. Its role in the maintenance of the intestinal epithelium is of particular interest, as several autophagy-related genes have been associated with intestinal disease. Autophagy and its regulatory mechanisms are involved in both homeostasis and repair of the intestine, supporting intestinal barrier function in response to cellular stress through tight junction regulation and protection from cell death. Read More

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Overcoming thickened pathological skin in psoriasis via iontophoresis combined with tight junction-opening peptide AT1002 for intradermal delivery of NF-κB decoy oligodeoxynucleotide.

Int J Pharm 2021 Apr 24;602:120601. Epub 2021 Apr 24.

Department of Pharmaceutical Health Chemistry, Graduate School of Biomedical Sciences, Tokushima University, Shomachi 1, Tokushima 770-8505, Japan. Electronic address:

Transdermal delivery of nucleic acid therapeutics has been demonstrated to be effective for psoriasis treatment. We previously reported the utility of iontophoresis (IP) using weak electric current (0.3-0. Read More

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Investigation of water bonding status of normal and psoriatic skin in vivo using diffuse reflectance spectroscopy.

Sci Rep 2021 Apr 26;11(1):8901. Epub 2021 Apr 26.

Department of Photonics, National Cheng Kung University, Tainan, 701, Taiwan, R.O.C..

Psoriasis affects more than 125 million people worldwide, and the diagnosis and treatment efficacy evaluation of the disease mainly rely on clinical assessments that could be subjective. Our previous study showed that the skin erythema level could be quantified using diffuse reflectance spectroscopy (DRS), and the hemoglobin concentration of most psoriatic lesion was higher than that of its adjacent uninvolved skin. While the compromised epidermal barrier function has been taken as the major cause of clinical manifestation of skin dryness and inflammation of psoriasis, very few methods can be used to effectively evaluate this function. Read More

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Triangulating molecular evidence to prioritize candidate causal genes at established atopic dermatitis loci.

J Invest Dermatol 2021 Apr 23. Epub 2021 Apr 23.

MRC Integrative Epidemiology Unit, Bristol Medical School, University of Bristol, Bristol, UK. Electronic address:

Genome-wide association studies for atopic dermatitis (AD) have identified 25 reproducible loci. We attempt to prioritize candidate causal genes at these loci using extensive molecular resources compiled into a bioinformatics pipeline. We identified a list of 103 molecular resources for AD aetiology, including expression, protein and DNA methylation QTL datasets in skin or immune-relevant tissues which were tested for overlap with GWAS signals. Read More

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Low threshold for cutaneous allergen sensitization but no spontaneous dermatitis or atopy in filaggrin-deficient mice.

J Invest Dermatol 2021 Apr 21. Epub 2021 Apr 21.

Institute for Immunology, Medical Faculty Carl Gustav Carus, University of Technology Dresden, Dresden, Germany. Electronic address:

Loss of filaggrin (FLG) causes Ichthyosis vulgaris. Reduced filaggrin expression compromises epidermal barrier function and is associated with atopic dermatitis, allergy and asthma. The flaky tail mouse harbors two mutations that affect the skin barrier, Flg, resulting in hypomorphic FLG expression, and Tmem79 inactivating transmembrane protein 79. Read More

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A unique mode of keratinocyte death requires intracellular acidification.

Proc Natl Acad Sci U S A 2021 Apr;118(17)

Laboratory for Skin Homeostasis, RIKEN Center for Integrative Medical Sciences, Yokohama 230-0045, Japan;

The stratum corneum (SC), the outermost epidermal layer, consists of nonviable anuclear keratinocytes, called corneocytes, which function as a protective barrier. The exact modes of cell death executed by keratinocytes of the upper stratum granulosum (SG1 cells) remain largely unknown. Here, using intravital imaging combined with intracellular Ca- and pH-responsive fluorescent probes, we aimed to dissect the SG1 death process in vivo. Read More

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Exploring the dermotoxicity of the mycotoxin deoxynivalenol: combined morphologic and proteomic profiling of human epidermal cells reveals alteration of lipid biosynthesis machinery and membrane structural integrity relevant for skin barrier function.

Arch Toxicol 2021 Apr 23. Epub 2021 Apr 23.

Department of Food Chemistry and Toxicology, Faculty of Chemistry, University of Vienna, Währingerstr. 38-40, 1090, Vienna, Austria.

Deoxynivalenol (vomitoxin, DON) is a secondary metabolite produced by Fusarium spp. fungi and it is one of the most prevalent mycotoxins worldwide. Crop infestation results not only in food and feed contamination, but also in direct dermal exposure, especially during harvest and food processing. Read More

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PLGA based film forming systems for superficial fungal infections treatment.

Eur J Pharm Sci 2021 Apr 16;163:105855. Epub 2021 Apr 16.

Department of Biological and Medical Sciences, Charles University, Faculty of Pharmacy in Hradec Kralove, Akademika Heyrovskeho 1203, Hradec Kralove 500 05, Czech Republic.

As proven in clinical trials, superficial fungal infections can be effectively treated by single topical application of terbinafine hydrochloride (Ter-HCl) in a film forming system (FFS). Poly(lactic-co-glycolic acid) (PLGA) derivatives, originally synthesized with intention to get carriers with optimized properties for drug delivery, and multifunctional plasticizers - ethyl pyruvate, methyl salicylate, or triacetin - were used for formulation of Ter-HCl loaded FFSs. After spraying, a biodegradable, transparent, adhesive, and occlusive thin layer is formed on the skin, representing drug depot. Read More

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