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Genotype and virulence gene analyses of Bacillus cereus group clinical isolates from the Chinese softshell turtle (Pelodiscus sinensis) in Taiwan.

J Fish Dis 2021 Jun 14. Epub 2021 Jun 14.

Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan.

Chinese softshell turtles (Pelodiscus sinensis) (CST) are susceptible to infections by bacteria belonging to the Bacillus cereus group (Bcg). Bcg includes several closely related species, two of which, B. cereus and B. Read More

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Development and application of ubiquitin-based chemical probes.

Chem Sci 2020 Aug 4;11(47):12633-12646. Epub 2020 Aug 4.

Tsinghua-Peking Center for Life Sciences, Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Center for Synthetic and Systems Biology, Department of Chemistry, Tsinghua University Beijing 100084 China

Protein ubiquitination regulates almost every process in eukaryotic cells. The study of the many enzymes involved in the ubiquitination system and the development of ubiquitination-associated therapeutics are important areas of current research. Synthetic tools such as ubiquitin-based chemical probes have been making an increasing contribution to deciphering various biochemical components involved in ubiquitin conjugation, recruitment, signaling, and deconjugation. Read More

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Molecular Basis for the Interactions of Human Thioredoxins with Their Respective Reductases.

Oxid Med Cell Longev 2021 1;2021:6621292. Epub 2021 Jun 1.

Institute for Medical Biochemistry and Molecular Biology, University Medicine Greifswald, Germany.

The mammalian cytosolic thioredoxin (Trx) system consists of Trx1 and its reductase, the NADPH-dependent seleno-enzyme TrxR1. These proteins function as electron donor for metabolic enzymes, for instance in DNA synthesis, and the redox regulation of numerous processes. In this work, we analysed the interactions between these two proteins. Read More

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Tailor-made novel electrospun polystyrene/poly(d,l-lactide-co-glycolide) for oxidoreductases immobilization: Improvement of catalytic properties under extreme reaction conditions.

Bioorg Chem 2021 Jun 1;114:105036. Epub 2021 Jun 1.

Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, PL-60965 Poznan, Poland. Electronic address:

Immobilized enzymes find applications in many areas such as pharmacy, medicine, food production and environmental protection. However, protecting these biocatalysts against harsh reaction conditions and retaining their enzymatic activity even after several biocatalytic cycles are major challenges. Properly selected supports and type of surface modifier therefore seem to be crucial for achieving high retention of catalytic activity of immobilized biomolecules. Read More

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SULT genetic polymorphisms: physiological, pharmacological and clinical implications.

Expert Opin Drug Metab Toxicol 2021 Jun 10. Epub 2021 Jun 10.

Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, University of Toledo Health Science Campus, Toledo, OH 43614 USA.

Introduction: Cytosolic sulfotransferases (SULTs)-mediated sulfation is critically involved in the metabolism of key endogenous compounds such as catecholamines and thyroid/steroid hormones, as well as a variety of drugs and other xenobiotics. Studies performed in the past three decades have yielded a good understanding about the enzymology of the SULTs and their structural biology, phylogenetic relationships, tissue/organ-specific/developmental expression, as well as the regulation of the gene expression. An emerging area is related to the functional impact of the genetic polymorphisms. Read More

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Behavioural tagging: Effect of novelty exploration on plasticity related molecular signatures.

Exp Brain Res 2021 Jun 7. Epub 2021 Jun 7.

Department of Toxicology, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi, 110062, India.

Learning and memory are one of those frontier areas of neurobiology which attract us to investigate the intricacy of this process. Here, we aimed to investigate the general mechanism of "Behavioural Tagging and Capture" in long term memory (LTM) formation and to find the key factors playing role in consolidation of LTM. In this study, we've shown that not only plasticity related proteins (PRPs) but neurotransmitters and immediate early genes (IEGs) also play an important role in memory formation process. Read More

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Glycoside hydrolase stabilization of transition state charge: new directions for inhibitor design.

Chem Sci 2020 Sep 16;11(38):10488-10495. Epub 2020 Sep 16.

Department of Chemistry, Simon Fraser University Burnaby British Columbia V5A 1S6 Canada +1-778-782-8814.

Carbasugars are structural mimics of naturally occurring carbohydrates that can interact with and inhibit enzymes involved in carbohydrate processing. In particular, carbasugars have attracted attention as inhibitors of glycoside hydrolases (GHs) and as therapeutic leads in several disease areas. However, it is unclear how the carbasugars are recognized and processed by GHs. Read More

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September 2020

Methyl jasmonate ameliorates lead toxicity in by modulating chlorophyll metabolism, antioxidative capacity and metal translocation.

Physiol Mol Biol Plants 2021 May 20;27(5):1089-1104. Epub 2021 Apr 20.

Department of Agronomy, Islamic Azad University of Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran.

Lead (Pb) not only negatively alters plant growth and yield but may also have potentially toxic risks to human health. Nevertheless, the interaction between rice ( L.) plants and the molecular cell dynamics induced by lead-methyl jasmonate (MJ) remains unknown. Read More

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Potential strategies to prevent encrustations on urinary stents and catheters - thinking outside the box: a European network of multidisciplinary research to improve urinary stents (ENIUS) initiative.

Expert Rev Med Devices 2021 Jun 11:1-9. Epub 2021 Jun 11.

ARTORG Center for Biomedical Engineering Research, University of Bern, Bern, Switzerland.

Urinary stents have been around for the last 4 decades, urinary catheters even longer. They are associated with infections, encrustation, migration, and patient discomfort. Research efforts to improve them have shifted onto molecular and cellular levels. Read More

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[The role of carbonic anhydrase IX in the progression of malignant tumors - a potential therapeutic target?]

Magy Onkol 2021 Jun 20;65(2):157-166. Epub 2021 May 20.

ÁOK, Debreceni Egyetem, Patológiai Intézet, Debrecen, Hungary.

Insufficient tissue perfusion in malignancies results in hypoxic areas, favoring neoplastic progression. Tumor cells under hypoxia undergo an adaptive program by activating alternative metabolic pathways, which is regulated by hypoxia inducible factor-1 (HIF1) in order to overcome microenvironmental changes. The expression of carbonic anhydrase IX (CAIX) is a prominent protective mechanism against intracellular acidosis occurring in cancer cells suffering from hypoxia. Read More

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Gallic acid suppresses inflammation and oxidative stress through modulating Nrf2-HO-1-NF-κB signaling pathways in elastase-induced emphysema in rats.

Environ Sci Pollut Res Int 2021 Jun 2. Epub 2021 Jun 2.

Persian Gulf Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Emphysema is associated with an abnormal airspace enlargement distal to the terminal bronchioles accompanied by destructive changes in the alveolar walls and chronic inflammation. Air pollution can cause respiratory diseases such as chronic obstructive pulmonary disease (COPD) and emphysema in urban areas. As a natural antioxidant compound, gallic acid may be effective in controlling inflammation and preventing disease progression. Read More

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Cannabinoid Signaling in Auditory Function and Development.

Front Mol Neurosci 2021 17;14:678510. Epub 2021 May 17.

Department of Neurobiology and Anatomical Sciences, University of Mississippi Medical Center, Jackson, MS, United States.

Plants of the genus have been used by humans for millennia for a variety of purposes. Perhaps most notable is the use of certain strains for their psychoactive effects. More recently, several biologically active molecules within the plants of these strains, called phytocannabinoids or simply cannabinoids, have been identified. Read More

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Changes in energetic metabolism and lysosomal destruction in the skeletal muscle and cardiac tissues of pigeons (Columba livia f. urbana) from urban areas of the northern Pomeranian region (Poland).

Ecotoxicology 2021 Jun 2. Epub 2021 Jun 2.

Department of Physics, Institute of Science and Technology, Pomeranian University in Słupsk, Słupsk, Poland.

The aim of the present study was to evaluate the biochemical responses of the skeletal muscle and cardiac tissues of the urban pigeon as a bioindicator organism tested in diverse environments (Szpęgawa as a rural environment and Słupsk as an urban environment, Pomeranian Voivodeship, northern Poland), resulting in changes in the level of lipid peroxidation at the initial and final stages of this process and the activities of Krebs cycle enzymes (succinate dehydrogenase, pyruvate dehydrogenase, isocitrate dehydrogenase, and alfa-ketoglutarate dehydrogenase). Szpęgawa village was chosen due to the intensive use of the European motorway A1 with significant traffic and pollution levels. The concentration of Pb was higher in the soil and feathers of pigeons nesting in the polluted areas (Szpęgawa). Read More

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Overexpression of Enhances Drought Tolerance by Alleviating Oxidative Damage and Promoting Water Uptake in Transgenic Apple.

Int J Mol Sci 2021 May 24;22(11). Epub 2021 May 24.

State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, China.

Water deficit adversely affects apple () productivity on the Loess Plateau. Autophagy plays a key role in plant responses to unfavorable environmental conditions. Previously, we demonstrated that a core apple autophagy-related protein, , was responsive to various stresses at the transcript level. Read More

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The Cellular and Chemical Biology of Endocytic Trafficking and Intracellular Delivery-The GL-Lect Hypothesis.

Authors:
Ludger Johannes

Molecules 2021 May 31;26(11). Epub 2021 May 31.

Cellular and Chemical Biology Department, Institut Curie, PSL Research University, U1143 INSERM, UMR3666 CNRS, 26 rue d'Ulm, CEDEX 05, 75248 Paris, France.

Lipid membranes are common to all forms of life. While being stable barriers that delimitate the cell as the fundamental organismal unit, biological membranes are highly dynamic by allowing for lateral diffusion, transbilayer passage via selective channels, and in eukaryotic cells for endocytic uptake through the formation of membrane bound vesicular or tubular carriers. Two of the most abundant fundamental fabrics of membranes-lipids and complex sugars-are produced through elaborate chains of biosynthetic enzymes, which makes it difficult to study them by conventional reverse genetics. Read More

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Cytogenetic, Serum Liver Enzymes and Liver Cell Pathology of the Hampala Barb Fish ) Affected by Toxic Elements in the Contaminated Nam Kok River Near the Sepon Gold-Copper Mine, Lao PDR.

Int J Environ Res Public Health 2021 May 29;18(11). Epub 2021 May 29.

Division of Environmental Science, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.

This study aimed to determine toxic element concentrations in aquatic environments, including water and sediment, and in the fish, and to evaluate chromosome abnormalities, serum liver enzyme changes and liver histopathological alterations in from the Nam Kok River near the Sepon gold-copper mine, Lao People's Democratic Republic, as compared with a control area without mining activity. The results revealed significant differences ( < 0.05) in As, Ba, Cu, Fe, Mn, Ni, Se and Zn in water, in all of the studied potentially toxic elements in sediment, and in As, Ba, Cd, Cr, Cu, Mn, Ni, Se, and Zn in the fish between the study and control areas. Read More

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Heat Priming of Lentil ( Medik.) Seeds and Foliar Treatment with γ-Aminobutyric Acid (GABA), Confers Protection to Reproductive Function and Yield Traits under High-Temperature Stress Environments.

Int J Mol Sci 2021 May 29;22(11). Epub 2021 May 29.

Department of Botany, Panjab University, Chandigarh 160014, India.

Gradually increasing temperatures at global and local scales are causing heat stress for cool and summer-season food legumes, such as lentil ( Medik.), which is highly susceptible to heat stress, especially during its reproductive stages of development. Hence, suitable strategies are needed to develop heat tolerance in this legume. Read More

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Differential Salt Tolerance Strategies in Three Halophytes from the Same Ecological Habitat: Augmentation of Antioxidant Enzymes and Compounds.

Plants (Basel) 2021 May 30;10(6). Epub 2021 May 30.

Botany & Microbiology Department, Faculty of Science (Cairo), Al-Azhar University, Madinat Nasr, Cairo 11751, Egypt.

Understanding the salt tolerance mechanism in obligate halophytes provides valuable information for conservation and re-habitation of saline areas. Here, we investigated the responses of three obligate halophytes namely , and to salt stress (0, 100, 200, 400 and 600 mM NaCl) during their vegetative growth with regard to biomass, ions contents (Na, K and Ca), chlorophyll contents, carotenoids, phenolic compounds, flavonoids, and superoxide dismutase, peroxidase and esterase activities. showed the lowest biomass, root K content, Chl a/b ratio, and carotenoids under salinity. Read More

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LC-MS/MS Profiles and In Vitro Biological Activities of Extracts of an Endemic Species from Turkey: ssp. .

Plants (Basel) 2021 May 25;10(6). Epub 2021 May 25.

Faculty of Pharmacy, Afyonkarahisar Health Sciences University, Afyonkarahisar 03150, Turkey.

: Genus is one of the largest of the family, having around 300 different plant species inhabiting areas with temperate and warm climates. The species in Turkey are represented with 81 taxa; 51 of them being endemic. Plants of the genus have been known for their biological activity and their use in ethnomedicine. Read More

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Lack of WWC2 Protein Leads to Aberrant Angiogenesis in Postnatal Mice.

Int J Mol Sci 2021 May 18;22(10). Epub 2021 May 18.

Department of Ophthalmology, University of Münster Medical School, 48149 Münster, Germany.

The WWC protein family is an upstream regulator of the Hippo signalling pathway that is involved in many cellular processes. We examined the effect of an endothelium-specific WWC1 and/or WWC2 knock-out on ocular angiogenesis. Knock-outs were induced in C57BL/6 mice at the age of one day (P1) and evaluated at P6 (postnatal mice) or induced at the age of five weeks and evaluated at three months of age (adult mice). Read More

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Unveiling the Impact of Antibiotics and Alternative Methods for Animal Husbandry: A Review.

Antibiotics (Basel) 2021 May 13;10(5). Epub 2021 May 13.

Novel Bacteria and Drug Discovery (NBDD) Research Group, Microbiome and Bioresource Research Strength (MBRS), Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Selangor Darul Ehsan 47500, Malaysia.

Since the 1950s, antibiotics have been used in the field of animal husbandry for growth promotion, therapy and disease prophylaxis. It is estimated that up to 80% of the antibiotics produced by the pharmaceutical industries are used in food production. Most of the antibiotics are used as feed additives at sub-therapeutic levels to promote growth. Read More

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Dysregulation of miR-15a-5p, miR-497a-5p and miR-511-5p Is Associated with Modulation of BDNF and FKBP5 in Brain Areas of PTSD-Related Susceptible and Resilient Mice.

Int J Mol Sci 2021 May 13;22(10). Epub 2021 May 13.

Section of Pharmacology, Department of Biomedical and Biotechnological Sciences, University of Catania, 95123 Catania, Italy.

Post-traumatic stress disorder (PTSD) is a neuropsychiatric disorder occurring in susceptible individuals following a traumatic event. Understanding the mechanisms subserving trauma susceptibility/resilience is essential to develop new effective treatments. Increasing evidence suggests that non-coding RNAs, such as microRNAs (miRNAs), may play a prominent role in mediating trauma susceptibility/resilience. Read More

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Recent Advancements in Enzyme-Based Lateral Flow Immunoassays.

Sensors (Basel) 2021 May 12;21(10). Epub 2021 May 12.

Department of Chemistry "Giacomo Ciamician", Alma Mater Studiorum-University of Bologna, Via Selmi 2, 40126 Bologna, Italy.

Paper-based lateral-flow immunoassays (LFIAs) have achieved considerable commercial success and their impact in diagnostics is continuously growing. LFIA results are often obtained by visualizing by the naked eye color changes in given areas, providing a qualitative information about the presence/absence of the target analyte in the sample. However, this platform has the potential to provide ultrasensitive quantitative analysis for several applications. Read More

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Serine/Threonine Phosphatases in LTP: Two B or Not to Be the Protein Synthesis Blocker-Induced Impairment of Early Phase.

Int J Mol Sci 2021 May 4;22(9). Epub 2021 May 4.

Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, 117485 Moscow, Russia.

Dephosphorylation of target proteins at serine/threonine residues is one of the most crucial mechanisms regulating their activity and, consequently, the cellular functions. The role of phosphatases in synaptic plasticity, especially in long-term depression or depotentiation, has been reported. We studied serine/threonine phosphatase activity during the protein synthesis blocker (PSB)-induced impairment of long-term potentiation (LTP). Read More

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[Treatment of Renal Artery Perforation with Covered Stent after Percutaneous Biopsy of Pancreatic Head].

Dtsch Med Wochenschr 2021 Jun 1;146(11):759-762. Epub 2021 Jun 1.

Klinik für Urologie, RKH Klinikum Ludwigsburg.

History And Clinical Finding:  In a 67-year-old female patient with upper abdominal pain, computed tomography showed a partly calcified swelling of the pancreatic head and wall thickening of the duodenum.

Examinations:  Inpatient physical examination findings were normal. Laboratory showed increased pancreatic enzymes (amylase 210 U/l [Standard range: 28-100 U/l], lipase 2115 U/l [Standard range: 23-300 U/l]) and inflammation values (CRP 11. Read More

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Effects of novel bioorganic fertilizer application on soil enzymes and bacterial community in multi-site rice paddies in China.

AMB Express 2021 May 31;11(1):79. Epub 2021 May 31.

Hunan Provincial Key Laboratory for Biology and Control of Weeds, Hunan Academy of Agricultural Sciences, Changsha, 410125, China.

Application of a novel bioorganic fertilizer (BIO) has been effectively used to inhibit weeds in rice paddies. To identify changes in soil bacterial community and enzymes in response to BIO treatments, field experiments were carried out in five major rice-growing areas in China. The dominant phylogenetic groups recorded included Proteobacteria, Actinobacteria, Bacteroidetes, Firmicutes and Acidobacteria. Read More

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(Poly)phenol-Rich Diets in the Management of Endothelial Dysfunction in Diabetes Mellitus: Biological Properties in Cultured Endothelial Cells.

Mol Nutr Food Res 2021 May 29:e2001130. Epub 2021 May 29.

REQUIMTE/LAQV, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, Porto, 4169-007, Portugal.

Processed and ready-to-eat foods became routinely consumed resulting in a sharp rise of sugar intake in people's daily diets. The inclusion of fresh fruits and vegetables rich in (poly)phenols has been encouraged by the World Health Organisation as part of our daily choices to ameliorate endothelial dysfunction and ease the socio-economic burden of diabetes. Research in Food, Nutrition and Cell Metabolism areas is revealing that the health benefits of (poly)phenol-rich foods go beyond their antioxidant properties and are in fact key modulators of redox and glycaemia status, and inflammatory response contributing to improved endothelial function and vascular health in diabetes. Read More

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Gender differences in use of invasive diagnostic and therapeutic procedures for acute ischaemic heart disease in Chinese adults.

Heart 2021 May 27. Epub 2021 May 27.

Health Economics Research Centre, Nuffield Department of Population Health, University of Oxford, Oxford, UK

Objective: To investigate gender differences in the use of diagnostic and therapeutic procedures for acute ischaemic heart disease (IHD) in Chinese adults and assess whether socioeconomic or health system factors contribute to such differences.

Methods: In 2004-2008, the China Kadoorie Biobank recruited 512 726 adults from 10 diverse areas in China. Data for 38 928 first hospitalisations with IHD (2911 acute myocardial infarction (AMI), 9817 angina and 26 200 other IHD) were obtained by electronic linkage to health insurance records until 31 December 2016. Read More

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Identifying potential drug targets and candidate drugs for COVID-19: biological networks and structural modeling approaches.

F1000Res 2021;10:127. Epub 2021 Feb 18.

Centre of Interdisciplinary Science-Computational Life Sciences, College of Chemistry and Chemical Engineering,, Henan University of Technology, Zhengzhou, Henan, 450001, China.

Coronavirus (CoV) is an emerging human pathogen causing severe acute respiratory syndrome (SARS) around the world. Earlier identification of biomarkers for SARS can facilitate detection and reduce the mortality rate of the disease. Thus, by integrated network analysis and structural modeling approach, we aimed to explore the potential drug targets and the candidate drugs for coronavirus medicated SARS. Read More

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Chemical probes targeting the kinase CK2: a journey outside the catalytic box.

Org Biomol Chem 2021 05;19(20):4380-4396

Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.

CK2 is a protein kinase that plays important roles in many physio-pathological cellular processes. As such, the development of chemical probes for CK2 has received increasing attention in the past decade with more than 40 lead compounds developed. In this review, we aim to provide the reader with a comprehensive overview of the chemical probes acting outside the highly-conserved ATP-site developed to date. Read More

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