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ADIPOCYTOKINES LEPTIN AND ADIPONEСTIN AS PREDICTORS OF GENERALIZED PERIODONTITIS ASSOCIATED WITH OBESITY.

Georgian Med News 2021 Mar(312):42-46

Bogomolets National Medical University, Institute of Postgraduate Education, Department of Dentistry, Kyiv, Ukraine.

In recent years, according to the WHO, more than 1,9 billion people over the age of 18 are overweight, of whom more than 650 million are obese, with an annual trend. Studies in recent decades have shown that obese young people with generalized periodontitis have abnormalities in protein, fat and carbohydrate metabolism, which contributes to the early destruction of the periodontal tissue complex and rapid tooth loss. The aim of our study was to examine changes in serum leptin and adiponectin in patients with generalized periodontitis on the background of obesity. Read More

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Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation.

Redox Biol 2021 Apr 30;43:101994. Epub 2021 Apr 30.

Laboratory of Mitochondria and Metabolism, Department of Anesthesiology, National Clinical Research Center for Geriatrics, West China Hospital of Sichuan University, Chengdu, 610041, China; Laboratory of Anesthesia and Critical Care Medicine, West China Hospital of Sichuan University, Chengdu, 610041, China. Electronic address:

Ischemia-reperfusion (I/R) injury is detrimental to cardiovascular system. Alteration in glucose metabolism has been recognized as an important adaptive response under hypoxic conditions. However, the biological benefits underlying this metabolic phenotype remain to be elucidated. Read More

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New indenopyrazole linked oxadiazole conjugates as anti-pancreatic cancer agents: Design, synthesis, in silico studies including 3D-QSAR analysis.

Bioorg Med Chem Lett 2021 May 5:128094. Epub 2021 May 5.

Organic Synthesis and Process Chemistry Division, CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad 500007, India; Academy of Scientific and Innovative Research, Ghaziabad 201002, India; School of Pharmaceutical Education and Research (SPER), Jamia Hamdard, New Delhi 110062, India. Electronic address:

To continue the quest of newer anticancer agents, herein a novel class of 1,4-Dihydroindenopyrazole linked oxadiazole conjugates 9(a-r) was designed, synthesized and experimented for their anti-proliferative activities against four different cancer cell lines (human) such as MDA MB-231 (breast), PANC-1 (pancreatic), MCF-7 (breast), and Caco-2 (Colorectal) by using MTT assay. Among the series compound 9h and 9m demonstrated significant potency against the PANC-1 (human pancreatic cancer cells) with IC value 7.4 μM and 4. Read More

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Effects of a combination of Mass and Dutch variant as an inactivated vaccine against variant 2 avian infectious bronchitis virus challenge.

Microb Pathog 2021 May 5:104937. Epub 2021 May 5.

Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

The present study aimed to evaluate the effects of inactivated vaccines combining Mass and Dutch variants as vaccine boosters after H120 priming on inhibiting variant 2 viral load in the kidneys (as the target organ) and reducing fecal shedding. Ciliostasis score and antibody response were investigated as well. A total of 150 specific-pathogen-free (SPF) chicken were divided into six groups. Read More

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Assessment of progesterone synthesis and its regulation role on dihydrotestosterone secretion in sheep epididymis.

Gene 2021 May 5:145699. Epub 2021 May 5.

Animal Science and Technology College, Gansu Agricultural University, Lanzhou, China. Electronic address:

Progesterone (P4) is an anti-androgen compound whose role in sperm maturation and functionality remains unclear in sheep. Here, we aimed to investigate the regulation mechanism of P4 on the epididymal secretion of dihydrotestosterone (DHT). To this end, we performed enzyme-linked immunosorbent assays, immunohistochemical staining, western blotting, and quantitative real-time polymerase chain reaction to detect P4 concentration as well as StAR, P450scc, and 3β-HSD expression in sheep epididymis. Read More

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Recombinant Lactobacillus plantarum NC8 strain expressing porcine rotavirus VP7 induces specific antibodies in BALB/c mice.

Acta Biochim Biophys Sin (Shanghai) 2021 May 8. Epub 2021 May 8.

College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun 130118, China.

The major etiologic agent that causes acute gastroenteritis worldwide in young animals and children is Group A rotavirus. Currently, commercially available vaccines do not often prevent porcine rotavirus (PRV) infection. In this study, we evaluated the efficacy of oral recombinant Lactobacillus vaccine against PRV in a mouse model. Read More

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Half-life modeling of basic fibroblast growth factor released from growth factor-eluting polyelectrolyte multilayers.

Sci Rep 2021 May 7;11(1):9808. Epub 2021 May 7.

Department of Plastics Engineering, University of Massachusetts Lowell, One University Ave, Lowell, MA, 01854, USA.

Growth factor-eluting polymer systems have been widely reported to improve cell and tissue outcomes; however, measurements of actual growth factor concentration in cell culture conditions are limited. The problem is compounded by a lack of knowledge of growth factor half-lives, which impedes efforts to determine real-time growth factor concentrations. In this work, the half-life of basic fibroblast growth factor (FGF2) was determined using enzyme linked immunosorbent assay (ELISA). Read More

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Alternative glycosylation controls endoplasmic reticulum dynamics and tubular extension in mammalian cells.

Sci Adv 2021 May 7;7(19). Epub 2021 May 7.

Department of Biomolecular Medicine and Center for Medical Genetics, Ghent University, Ghent, Belgium.

The endoplasmic reticulum (ER) is a central eukaryotic organelle with a tubular network made of hairpin proteins linked by hydrolysis of guanosine triphosphate nucleotides. Among posttranslational modifications initiated at the ER level, glycosylation is the most common reaction. However, our understanding of the impact of glycosylation on the ER structure remains unclear. Read More

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NEDD4 regulates ubiquitination and stability of the cell adhesion molecule IGPR-1 via lysosomal pathway.

J Biomed Sci 2021 May 7;28(1):35. Epub 2021 May 7.

Department of Pathology, School of Medicine, Boston University Medical Campus, 670 Albany Street, Boston, MA, 02118, USA.

Background: The cell adhesion molecule IGPR-1 regulates various critical cellular processes including, cell-cell adhesion, mechanosensing and autophagy and plays important roles in angiogenesis and tumor growth; however, the molecular mechanism governing the cell surface levels of IGPR-1 remains unknown.

Results: In the present study, we used an in vitro ubiquitination assay and identified ubiquitin E3 ligase NEDD4 and the ubiquitin conjugating enzyme UbcH6 involved in the ubiquitination of IGPR-1. In vitro GST-pulldown and in vivo co-immunoprecipitation assays demonstrated that NEDD4 binds to IGPR-1. Read More

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An automated approach to determine antibody endpoint titers for COVID-19 by an enzyme-linked immunosorbent assay.

Immunohematology 2021 Mar;37(1):33-43

Center for Transfusion Medicine and Cellular Therapies, and Department of Pathology and Laboratory Medicine, Emory University School of Medicine , 201 Dowman Drive, Atlanta, GA 30322 , and Joint Program in Transfusion Medicine, Department of Pathology, Brigham and Women's Hospital, Harvard Medical School , 630E New Research Building, 77 Avenue Louis Pasteur, Boston, MA 02115.

While a variety of therapeutic options continue to emerge for COVID-19 treatment, convalescent plasma (CP) has been used as a possible treatment option early in the pandemic. One of the most significant challenges with CP therapy, however, both when defining its efficacy and implementing its approach clinically, is accurately and efficiently characterizing an otherwise heterogenous therapeutic treatment. Given current limitations, our goal is to leverage a SARS antibody testing platform with a newly developed automated endpoint titer analysis program to rapidly define SARS-CoV-2 antibody levels in CP donors and hospitalized patients. Read More

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Combining urine antigen and blood polymerase chain reaction for the diagnosis of disseminated histoplasmosis in hospitalized patients with advanced HIV disease.

Med Mycol 2021 May 7. Epub 2021 May 7.

Laboratório de Biologia Molecular de Parasitas e Fungos, Instituto Adolfo Lutz, São Paulo 01246-000, Brazil.

Disseminated histoplasmosis (DH) is endemic in Latin America and the Caribbean where diagnostic tools are restricted. We carried-out a 1-year prospective cohort study at a referral hospital in São Paulo, Brazil. Participants had > or =18 years old, were hospitalized due to any indication and had CD4+ < 200 cells/µl. Read More

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Influence of bisphosphonates on oral implantology: Sodium alendronate and zoledronic acid enhance the synthesis and activity of matrix metalloproteinases by gingival fibroblasts seeded on titanium.

Arch Oral Biol 2021 Apr 24;127:105134. Epub 2021 Apr 24.

Department of Physiology and Pathology, São Paulo State University (UNESP), School of Dentistry, Araraquara, SP, Brazil.

Objective: This study aimed to assess the influence of the bisphosphonates zoledronic acid and sodium alendronate on MMP-2 and MMP-9 synthesis and activity by gingival fibroblasts seeded onto titanium substrate.

Design: Titanium discs were placed in 24-well cell culture plates and gingival fibroblasts were seeded (1 × 10 cells/discs) on them using Dulbecco's Modified Eagle's Medium (DMEM) + 10 % fetal bovine serum (FBS) for 24 h. After this period, a fresh serum-free DMEM containing zoledronic acid or sodium alendronate at 0. Read More

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Adenosine Kinase: An Epigenetic Modulator in Development and Disease.

Neurochem Int 2021 May 4:105054. Epub 2021 May 4.

Department of Neurosurgery, Robert Wood Johnson Medical School, Rutgers University, Piscataway, NJ 08854; Department of Neurosurgery, New Jersey Medical School, Rutgers University, Newark, NJ 07102; Brain Health Institute, Rutgers University, Piscataway, NJ 08854. Electronic address:

Adenosine kinase (ADK) is the key regulator of adenosine and catalyzes the metabolism of adenosine to 5'-adenosine monophosphate. The enzyme exists in two isoforms: a long isoform (ADK-long, ADK-L) and a short isoform (ADK-short, ADK-S). The two isoforms are developmentally regulated and are differentially expressed in distinct subcellular compartments with ADK-L localized in the nucleus and ADK-S localized in the cytoplasm. Read More

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Association of COVID-19 transmission with high levels of ambient pollutants: Initiation and impact of the inflammatory response on cardiopulmonary disease.

Sci Total Environ 2021 Mar 16;779:146464. Epub 2021 Mar 16.

Division of Cardiology, Department of Medicine, David Geffen School of Medicine at University of California, Los Angeles, CA, United States of America; Department of Medicine, Greater Los Angeles VA Healthcare System, Los Angeles, CA, United States of America; Department of Bioengineering, Henry Samueli School of Engineering & Applied Science, University of California, Los Angeles, CA, United States of America. Electronic address:

Ambient air pollution contributes to 7 million premature deaths annually. Concurrently, the ongoing coronavirus disease 2019 (COVID-19) pandemic, complicated with S-protein mutations and other variants, caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has resulted in over 2.5 million deaths globally. Read More

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Establishment of a novel double-monoclonal antibody sandwich enzyme-linked immunosorbent assay (ELISA): Tool for human B7-H4 detection in autoimmune diseases.

Clin Exp Immunol 2021 May 7. Epub 2021 May 7.

Jiangsu Institute of Clinical Immunology & Jiangsu Key Laboratory of Clinical Immunology, The First Affiliated Hospital of Soochow University, Suzhou, China.

B7-H4, one of the immunoregulatory proteins, plays an inhibitory role by inhibiting T cell proliferation and cytokine production. Nevertheless, the significance of soluble B7-H4 (sB7-H4) in autoimmune diseases is unclear. In our study, we developed two novel mouse anti-human B7-H4 monoclonal antibodies (mAbs) (clone 8D4 and 7E1) with utilities for flow cytometry, immunoblotting and immunofluorescence. Read More

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A deep dive into the RecQ interactome: something old and something new.

Curr Genet 2021 May 7. Epub 2021 May 7.

Molecular and Cellular Biochemistry Department, Indiana University, Bloomington, IN, USA.

RecQ family helicases are found in all domains of life and play roles in multiple processes that underpin genomic integrity. As such, they are often referred to as guardians or caretakers of the genome. Despite their importance, however, there is still much we do not know about their basic functions in vivo, nor do we fully understand how they interact in organisms that encode more than one RecQ family member. Read More

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Effects of the Maillard reaction on the epitopes and immunoreactivity of tropomyosin, a major allergen in .

Food Funct 2021 May 7. Epub 2021 May 7.

College of Food and Biological Engineering, Xiamen Key Laboratory of Marine Functional Food, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University, Xiamen, Fujian 361021, China.

Scallop (Chlamys nobilis) causes an IgE-mediated food allergy; however, studies of the allergens in its musculus are not sufficiently comprehensive. In this context, the target protein was purified from scallops and confirmed to be the major allergen tropomyosin (TM) using proteomic technology and serological testing. Subsequently, seven potential IgE epitopes of TM were obtained using phage display technology with IgE enrichment from the serum of scallop-sensitized patients and identified via inhibition enzyme-linked immunosorbent assays. Read More

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Clinicians' pearls and myths in pemphigus.

Ital J Dermatol Venerol 2021 Apr;156(2):142-146

Department of Dermatology, Center for Blistering Diseases, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands.

Pemphigus comprises a heterogeneous group of autoimmune blistering diseases, which can affect both skin and mucous membranes, especially oral mucosa. This group of diseases shows usually a chronic-relapsing course. Since pemphigus is a rare disease, the diagnosis is often delayed, because it is based upon the recognition of consistent clinical, histologic, and direct immunofluorescence findings, as well as indirect immunofluorescence, and/or enzyme-linked immunosorbent assay. Read More

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Maintenance of neutralizing antibodies over ten months in convalescent SARS-CoV-2 afflicted patients.

Transbound Emerg Dis 2021 May 7. Epub 2021 May 7.

Dr. Gernot Walder GmbH, Medical Laboratory, Department of Virology, 9931, Ausservillgraten 30, Austria.

Knowledge of the level and duration of protective immunity against SARS-CoV-2 after primary infection is of crucial importance for preventive approaches. Currently, there is a lack of evidence on the persistence of specific antibodies. We investigated the generation and maintenance of neutralizing antibodies of convalescent SARS-CoV-2-afflicted patients over a ten-month period post-primary infection using an immunofluorescence assay, a commercial chemiluminescent immunoassay, and an in-house enzyme-linked neutralization assay. Read More

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Genotype-phenotype studies in a large cohort of Brazilian patients with Hunter syndrome.

Am J Med Genet C Semin Med Genet 2021 May 7. Epub 2021 May 7.

Medical Genetics Service, HCPA, Porto Alegre, Rio Grande do Sul, Brazil.

Mucopolysaccharidosis type II (MPS II) is an X-linked inherited disease caused by pathogenic variants in the IDS gene, leading to deficiency of the lysosomal enzyme iduronate-2-sulfatase and consequent widespread storage of glycosaminoglycans, leading to several clinical consequences, with progressive manifestations which most times includes cognitive decline. MPS II has wide allelic and clinical heterogeneity and a complex genotype-phenotype correlation. We evaluated data from 501 Brazilian patients diagnosed with MPS II from 1982 to 2020. Read More

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Amyloid β (Aβ) ELISA of Human iPSC-Derived Neuronal Cultures.

Methods Mol Biol 2021 May 7. Epub 2021 May 7.

Department of Physiology, Keio University, School of Medicine, Tokyo, Japan.

Amyloid β (Aβ) peptides are the main component of the characteristic insoluble deposits in brain parenchyma and small blood vessels in the patients afflicted with Alzheimer's disease (AD) and cerebral amyloid angiopathy (CAA). These small peptides are attributed to the pathogenesis of both AD and CAA, suggesting an important index for disease stage and progression. In the brain tissue, Aβs are released mainly from neuronal cells into extracellular space. Read More

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Cord blood levels of interleukin-10 decrease in neonates with increased birth weight: novel implications of the cytokine network in early obesity.

Eur J Pediatr 2021 May 7. Epub 2021 May 7.

Laboratory of Immunometabolism, Research Division, General Hospital of Mexico "Dr. Eduardo Liceaga", 06720, Mexico City, Mexico.

Interleukin-10 (IL-10) and interferon-gamma (IFN-gamma) are associated with body weight alterations in children, adolescents, and adults. However, little is known regarding the role of IL-10 and IFN-gamma in birth weight of neonates. One hundred eighty-two infants were enrolled and divided in groups of normal birth weight (< 95th percentile) or increased birth weight (> 95th percentile) for gestational age. Read More

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Evidence for Transmission of Taeniasis/Cysticercosis in a Rural Area of Northern Rwanda.

Front Vet Sci 2021 20;8:645076. Epub 2021 Apr 20.

Área de Parasitología del Departamento de Agroquímica y Medioambiente, Universidad Miguel Hernández de Elche, Alicante, Spain.

Cysticercosis is a parasitic infection caused by the metacestode larval stage (cysticercus) of . In humans, cysticercosis may infect the central nervous system and cause neurocysticercosis, which is responsible for over 50,000 deaths per year worldwide and is the major cause of preventable epilepsy cases, especially in low-income countries. Cysticercosis infection is endemic in many less developed countries where poor hygiene conditions and free-range pig management favor their transmission. Read More

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The Prevalence of Hepatitis B Virus Markers among Students of Shiraz University of Medical Sciences.

Adv Biomed Res 2021 26;10. Epub 2021 Feb 26.

Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Background: Protection against hepatitis B virus (HBV) is based on the presence of antibodies against hepatitis B surface antigen (HBsAg). Vaccination of newborns is the most effective means of prevention. This study aimed to evaluate the frequency of anti-HBs antibody (anti-HBsAb), anti-HB core Ab (anti-HBcAb), HBsAg, and HBV DNA among university students in Fars province, Southern Iran. Read More

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February 2021

The Circulating Midkine in the Newly Diagnosed Celiac Disease: Clinical Implications.

Adv Biomed Res 2021 27;10. Epub 2021 Jan 27.

Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Background: Celiac disease (CeD) is a chronic inflammatory small intestine disorder caused by an abnormal immune response to an array of the epitopes of the wheat gluten and related proteins of rye and barley in genetically susceptible individuals. Midkine (MK) is an angiogenic cytokine, chemotactic in the direction of polymorphonuclear neutrophils and macrophages, and a T-regulatory cell suppressor. So far, a possible relationship with CeD has not yet been explored. Read More

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January 2021

Arousing Effects of Electroacupuncture on the "Shuigou Point" in Rats with Disorder of Consciousness after Traumatic Brain Injury.

Evid Based Complement Alternat Med 2021 19;2021:6611461. Epub 2021 Apr 19.

The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510006, China.

Orexin is an important neuropeptide that stimulates cortical activation and arousal and is involved in the regulation of wakefulness and arousal. Our previous meta-analysis showed that acupuncture fared well in the treatment of TBI-induced DOC in which "shuigou (DU 26)" was the most important and frequent point targeted. In the present study, we investigated whether electroacupuncture (EA) promotes TBI-induced unconsciousness wakefulness via orexin pathway. Read More

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Soluble BTN2A1 Is a Potential Prognosis Biomarker in Pre-Treated Advanced Renal Cell Carcinoma.

Front Immunol 2021 20;12:670827. Epub 2021 Apr 20.

Team Immunity and Cancer, Centre de Recherche en Cancérologie de Marseille (CRCM), Inserm, U1068, CNRS, UMR7258, Institut Paoli-Calmettes, Aix-Marseille University, UM105, Marseille, France.

The development of immune checkpoint inhibitors (ICI) has dramatically changed the landscape of therapies for metastatic renal cell carcinoma. However, many patients do not benefit from such therapy and prognostic or predictive validated biomarker validated for ICI are still needed to better select and treat patient. Plasmatic soluble immune checkpoints have been described as potential immune biomarkers in hematological malignancies and solids tumors, then, we would like to explore the prognostic value of different soluble immune checkpoints in patients with mRCC treated with nivolumab after TKI. Read More

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Development of Vasoinhibin-Specific Monoclonal Antibodies.

Front Endocrinol (Lausanne) 2021 20;12:645085. Epub 2021 Apr 20.

Institute for Clinical Chemistry, Laboratory Medicine and Transfusion Medicine, Nuremberg General Hospital & Paracelsus Medical University, Nuremberg, Germany.

Vasoinhibin is a protein hormone with antiangiogenic, antivasodilatatory, and antivasopermeability effects generated by the proteolytic cleavage of prolactin. The discovery of its role in diabetic retinopathy and peripartum cardiomyopathy led to the evaluation of new pharmacological treatments in clinical interventional trials. However, the quantitative evaluation of vasoinhibin in biological samples from patients has not been possible due to the lack of vasoinhibin-specific antibodies. Read More

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The Predictive Value of Myeloperoxidase for Contrast-Induced Nephropathy After Percutaneous Coronary Intervention in Patients with Acute Myocardial Infarction.

Int J Gen Med 2021 30;14:1621-1629. Epub 2021 Apr 30.

Department of Cardiology, Zhongda Hospital of Southeast University Medical School, Nanjing, 210009, People's Republic of China.

Background: Higher serum myeloperoxidase (MPO) in patients with acute coronary syndrome is associated with adverse cardiovascular outcomes. Contrast-induced nephropathy (CIN) is associated with worse prognosis in patients with coronary artery disease following angiography. We have no idea whether patients with higher serum myeloperoxidase have a higher risk of CIN in acute myocardial infarction (AMI) after percutaneous coronary intervention (PCI). Read More

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Rotavirus gastroenteritis in Pakistan, 2018: updated disease burden.

BMC Infect Dis 2021 May 6;21(1):426. Epub 2021 May 6.

Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan.

Objective: Rotavirus A (RVA) is a significant cause of severe diarrheal illness and one of the common causes of death in children under the age of five. This study was aimed at detecting the prevalence of RVA in Pakistan after rotavirus vaccines were introduced. Fecal samples were obtained from 813 children from different hospitals in Rawalpindi and Islamabad, Pakistan, from January 2018 to December 2018. Read More

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