J Neuroimmune Pharmacol 2021 Apr 14. Epub 2021 Apr 14.
Department of Neurosciences, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health (F.C, A.U.), F.I., N.KdeR., A.U.) and Centre of Excellence for Biomedical Research (F.C., A.U.), University of Genova, Genoa, Italy.
Deoxycytidine kinase (dCK) and 5' deoxynucleotidase (NT5C2) are involved in metabolism of cladribine (2CdA), the immunomodulatory drug for multiple sclerosis; by mediating phosphorylation (activation) or phosphorolysis (deactivation) of 2CdA, respectively, these enzymes promote or prevent its accumulation in the cell, which leads to cell death. In particular, lymphocytes which present with a high intracellular dCK/NT5C2 ratio are more sensitive to 2CdA than other immune cells. We aim at determining if the expression of these enzymes and/or their activity differ in specific progenitor and mature immune cells and are influenced by cellular activation and/or exposure to 2CdA. Read More