164,056 results match your criteria enhanced gene

Copy number alterations identify a smoking-associated expression signature predictive of poor outcome in head and neck squamous cell carcinoma.

Cancer Genet 2021 May 28;256-257:136-148. Epub 2021 May 28.

Department of Biochemistry, Program in Cancer Cell Biology USA. Electronic address:

Cigarette smoking is a risk factor for the development of head and neck squamous cell carcinoma (HNSCC), partially due to tobacco-induced large-scale chromosomal copy-number alterations (CNAs). Identifying CNAs caused by smoking is essential in determining how gene expression from such regions impact tumor progression and patient outcome. We utilized The Cancer Genome Atlas (TCGA) whole genome sequencing data for HNSCC to directly identify amplified or deleted genes correlating with smoking pack-year based on linear modeling. Read More

View Article and Full-Text PDF

Synergistic adsorption and degradation of sulfamethoxazole from synthetic urine by hickory-sawdust-derived biochar: The critical role of the aromatic structure.

J Hazard Mater 2021 Jun 9;418:126366. Epub 2021 Jun 9.

Department of Civil Engineering, University of Louisiana at Lafayette, Lafayette, LA, 70504, USA; Energy Institute of Louisiana, University of Louisiana at Lafayette, Lafayette, LA, 70504, USA. Electronic address:

This study investigated the adsorptive removal and subsequent degradation of sulfamethoxazole (SMX) from a synthetic urine by biochar (BC). The BCs used in this study were prepared using two different feedstocks with different temperatures. Element analysis and Fourier transform infrared spectroscopy (FTIR) results suggested that the aromaticity of one of the BCs, 700HSBC was significantly different from the 700PSBC although both of them were prepared at the same temperature (700 °C) with similar pore size distributions and specific surface areas. Read More

View Article and Full-Text PDF

The antidepressant-like effects of Danggui Buxue Decoction in GK rats by activating CREB/BDNF/TrkB signaling pathway.

Phytomedicine 2021 May 21;89:153600. Epub 2021 May 21.

College of Traditional Chinese Medicine•College of Intergrated Chinese and Western Medicine, Nanjing University of Chinese Medicine, 138 Xianlin Rd, Nanjing, China. Electronic address:

Background: High rates of co-morbidity have been reported in patients with diabetes mellitus with depression (DD). Danggui Buxue Decoction (DBD), a Traditional Chinese Medicine formula composed of Angelica and Astragalus, has been historically used for the treatment of diabetes.

Purpose: This study aimed to investigated whether DBD and its main active component, ferulic acid (FA) from Angelica, could ameliorate depression-like behavior in DD and the underlying mechanisms. Read More

View Article and Full-Text PDF

Kaempferol inhibits benign prostatic hyperplasia by resisting the action of androgen.

Eur J Pharmacol 2021 Jun 12:174251. Epub 2021 Jun 12.

Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Poyang Lake Road 10, Tianjin 301617, China; Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Poyang Lake Road 10, Tianjin 301617, China; Laboratory of Pharmacology of TCM Formulae Co-Constructed By the Province-Ministry, Tianjin University of TCM, Poyang Lake Road 10, Tianjin 301617, China. Electronic address:

Kaempferol is a natural compound that inhibits tumor development in androgenic related prostate cancer. However, it is still not clear about its phyto-androgenic activity and whether it suppresses testosterone-induced benign prostatic hyperplasia (BPH) development. In this study, molecular docking, cellular immunofluorescence staining, chromatin immunoprecipitation and dual luciferase reporter assay were performed to investigate the androgenic activity of kaempferol. Read More

View Article and Full-Text PDF

Cytokinins initiate secondary growth in the Arabidopsis root through a set of LBD genes.

Curr Biol 2021 Jun 8. Epub 2021 Jun 8.

Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki 00014, Finland; Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, and Viikki Plant Science Centre, University of Helsinki, Helsinki 00014, Finland. Electronic address:

During primary growth, plant tissues increase their length, and as these tissues mature, they initiate secondary growth to increase thickness. It is not known what activates this transition to secondary growth. Cytokinins are key plant hormones regulating vascular development during both primary and secondary growth. Read More

View Article and Full-Text PDF

Co-delivery of Phagocytosis Checkpoint Silencer and Stimulator of Interferon Genes Agonist for Synergetic Cancer Immunotherapy.

ACS Appl Mater Interfaces 2021 Jun 15. Epub 2021 Jun 15.

School of Biomedical Sciences and Engineering, South China University of Technology, Guangzhou International Campus, Guangzhou 511442, P.R. China.

Efficient capture and presentation of tumor antigens by antigen-presenting cells (APCs), especially dendritic cells (DCs), are crucial for activating the anti-tumor immunity. However, APCs are immunosuppressed in the tumor microenvironment, which hinders the tumor elimination. To reprogram APCs for inducing strong anti-tumor immunity, we report here a co-delivery immunotherapeutic strategy targeting the phagocytosis checkpoint (signal regulatory protein α, SIRPα) and stimulator of interferon genes (STING) of APCs to jointly enhance their ability of capturing and presenting tumor antigens. Read More

View Article and Full-Text PDF

Vitamin C Inhibits the Metabolic Changes Induced by Tet1 Insufficiency Under High Fat Diet Stress.

Mol Nutr Food Res 2021 Jun 15:e2100417. Epub 2021 Jun 15.

Y. Yuan, X. Chen, Y. Sun, Y. Fan, L. Zheng, Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, 430072, China.

Scope: DNA methylation contributes to obesity, but the role of the DNA demethylase ten-eleven translocation protein 1 (Tet1) in obesity remains unclear. Vitamin C is a cofactor for the Tet family of proteins, but whether vitamin C can be used to treat obesity via Tet1 awaits clarification.

Methods And Results: Tet1 and Tet1 mice were fed a high fat diet (HFD). Read More

View Article and Full-Text PDF

Improving rice eating and cooking quality by coordinated expression of the major starch synthesis-related genes, SSII and Wx, in endosperm.

Plant Mol Biol 2021 Jun 15. Epub 2021 Jun 15.

Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, State Key Laboratory of Hybrid Rice, Key Laboratory of Plant Functional Genomics of the Ministry of Education, College of Agriculture, Yangzhou University, Yangzhou, 225009, China.

Key Message: Coordinated regulation of amylose and amylopectin synthesis via manipulation of SSII-2, SSII-3 and Wx expression in endosperm can improve rice eating and cooking quality. With increasing rice consumption worldwide, many researchers are working to increase the yield and improve grain quality, especially eating and cooking quality (ECQ). The rice ECQ is mainly controlled by the expression of starch synthesis-related genes (SSRGs) in endosperm. Read More

View Article and Full-Text PDF

Atherogenic oxoaldehyde of cholesterol induces innate immune response in monocytes and macrophages.

Cell Biochem Biophys 2021 Jun 15. Epub 2021 Jun 15.

Departments of Environmental Toxicology, College of Sciences and Engineering, Southern University and A & M College, Baton Rouge, LA, 70813, USA.

Cholesterol oxidation product, 3β-hydroxy-5-oxo-5,6-secocholestan-6-al (cholesterol 5,6-secosterol, ChSeco or Atheronal-A), formed at inflammatory sites, has been shown to promote monocyte differentiation into macrophages and cause elevated expression of macrophage scavenger receptors. Since inflammation is a key event at all stages of atherosclerotic plaque formation, the pro-inflammatory actions of ChSeco in human THP-1 monocytes and mouse J774 macrophages were investigated in the present study by employing ELISA, qRT-PCR, and functional assays. An increase in the secretion of interleukin-8 and platelet-derived growth factor (PDGF) isoform AA and, to a limited extent, PDGF isoform BB was observed into the culture medium of THP-1 monocytes exposed to ChSeco. Read More

View Article and Full-Text PDF

HBI Transcription Factor-Mediated ROS Homeostasis Regulates Nitrate Signal Transduction.

Plant Cell 2021 Jun 15. Epub 2021 Jun 15.

The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao, 266237, China.

Nitrate is both an important nutrient and a critical signaling molecule that regulates plant metabolism, growth, and development. Although several components of the nitrate signaling pathway have been identified, the molecular mechanism of nitrate signaling remains unclear. Here, we showed that the growth-related transcription factors HOMOLOG OF BRASSINOSTEROID ENHANCED EXPRESSION2 INTERACTING WITH IBH1 (HBI1) and its three closest homologs (HBIs) positively regulate nitrate signaling in Arabidopsis thaliana. Read More

View Article and Full-Text PDF

Transcriptional upregulation of host-specific terpene metabolism in aphid-induced galls of Pistacia palaestina.

J Exp Bot 2021 Jun 15. Epub 2021 Jun 15.

Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, The Volcani Center, Ramat Yishay, Israel.

Galling insects gain food and shelter by inducing specialized anatomical structures in their plant hosts. Such galls often accumulate plant defensive metabolites protecting the inhabiting insects from predation. We previously found that, despite a marked natural chemopolymorphism in natural populations of Pistacia palaestina, the monoterpene levels in Baizongia pistaciae induced galls is substantially higher than in leaves of their hosts. Read More

View Article and Full-Text PDF

The CaM1-Associated CCaMK-MKK1/6 cascade positively affects the lateral root growth through auxin signaling under salt stress in rice.

J Exp Bot 2021 Jun 15. Epub 2021 Jun 15.

MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, Huazhong Agricultural University, Wuhan, China.

CCaMKs and MAPKKs are two kinases that regulate salt stress response in plants. It remains unclear, however, how they cooperatively affect the lateral root growth under salt stress. Here, two conserved phosphorylation sites (S102 and T118) of OsCaM1 were identified, and affected the capability of binding to Ca 2+  in vitro and kinase activity of OsCCaMK in vivo. Read More

View Article and Full-Text PDF

Dynamic innate immune response determines susceptibility to SARS-CoV-2 infection and early replication kinetics.

J Exp Med 2021 Aug 15;218(8). Epub 2021 Jun 15.

Department of Laboratory Medicine, Yale School of Medicine, New Haven, CT.

Initial replication of SARS-CoV-2 in the upper respiratory tract is required to establish infection, and the replication level correlates with the likelihood of viral transmission. Here, we examined the role of host innate immune defenses in restricting early SARS-CoV-2 infection using transcriptomics and biomarker-based tracking in serial patient nasopharyngeal samples and experiments with airway epithelial organoids. SARS-CoV-2 initially replicated exponentially, with a doubling time of ∼6 h, and induced interferon-stimulated genes (ISGs) in the upper respiratory tract, which rose with viral replication and peaked just as viral load began to decline. Read More

View Article and Full-Text PDF

Studying human nociceptors: from fundamentals to clinic.

Brain 2021 Jun 15. Epub 2021 Jun 15.

Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford OX3 9DU, UK.

Chronic pain affects one in five of the general population and is the third most important cause of disability-adjusted life-years globally. Unfortunately, treatment remains inadequate due to poor efficacy and tolerability. There has been a failure in translating promising preclinical drug targets into clinic use. Read More

View Article and Full-Text PDF

The ER membrane protein complex subunit Emc3 controls angiogenesis via the FZD4/WNT signaling axis.

Sci China Life Sci 2021 Jun 10. Epub 2021 Jun 10.

Sichuan Provincial Key Laboratory for Human Disease Gene Study, Department of Medical Genetics, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, 610072, China.

The endoplasmic reticulum (ER) membrane protein complex (EMC) regulates the synthesis and quality control of membrane proteins with multiple transmembrane domains. One of the membrane spanning subunits, EMC3, is a core member of the EMC complex that provides essential hydrophilic vestibule for substrate insertion. Here, we show that the EMC subunit Emc3 plays critical roles in the retinal vascular angiogenesis by regulating Norrin/Wnt signaling. Read More

View Article and Full-Text PDF

A methyl esterase 1 (PvMES1) promotes the salicylic acid pathway and enhances Fusarium wilt resistance in common beans.

Theor Appl Genet 2021 Jun 14. Epub 2021 Jun 14.

Department of Agricultural and Environmental Sciences, Tennessee State University, Nashville, TN, 37209, USA.

Key Message: Methyl esterase (MES), PvMES1, contributes to the defense response toward Fusarium wilt in common beans by regulating the salicylic acid (SA) mediated signaling pathway from phenylpropanoid synthesis and sugar metabolism as well as others. Common bean (Phaseolus vulgaris L.) is an important food legume. Read More

View Article and Full-Text PDF

MiR-3614-5p Is a Potential Novel Biomarker for Colorectal Cancer.

Front Genet 2021 28;12:666833. Epub 2021 May 28.

Department of General Surgery, The Armed Police Corps Hospital of Anhui, Hefei, China.

MiR-3614-5p has been found in a variety of cancers including colorectal cancer. However, the association of miR-3614-5p with colorectal cancer is still unclear. Based on the Cancer Genome Atlas (TCGA) database, the relationship between miR-3614-5p and colorectal cancer can be proved. Read More

View Article and Full-Text PDF

Neuroprotective effects of bone marrow Sca-1 cells against age-related retinal degeneration in OPTN E50K mice.

Cell Death Dis 2021 Jun 15;12(6):613. Epub 2021 Jun 15.

Department of Ophthalmology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.

Glaucoma is characterized by retinal ganglion cell (RGC) death, the underlying mechanisms of which are still largely unknown. An E50K mutation in the Optineurin (OPTN) gene is a leading cause of normal-tension glaucoma (NTG), which directly affects RGCs in the absence of high intraocular pressure and causes severe glaucomatous symptoms in patients. Bone marrow (BM) stem cells have been demonstrated to play a key role in regenerating damaged tissue during ageing and disease through their trophic effects and homing capability. Read More

View Article and Full-Text PDF

Oxysterols Modify NLRP2 in Epithelial Cells, Identifying a Mediator of Ozone-induced Inflammation.

Am J Respir Cell Mol Biol 2021 Jun 14. Epub 2021 Jun 14.

University of North Carolina, Pediatrics, Chapel Hill, North Carolina, United States;

Ozone (O) is a prevalent air pollutant causing lung inflammation. Previous studies demonstrate that O oxidizes lipids, such as cholesterol, in the airway to produce oxysterols, such as secosterol-A (SecoA), which are electrophiles capable of forming covalent linkages preferentially with lysine residues and consequently modify protein function. The breadth of proteins modified by this oxysterol as well as the biological consequences in the lung are unknown. Read More

View Article and Full-Text PDF

Oocyte maturation with royal jelly increases embryo development and reduces apoptosis in goats.

Anim Reprod 2018 Aug 16;15(2):124-134. Epub 2018 Aug 16.

Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, IL, USA.

Royal jelly (RJ) was supplemented to goat oocyte maturation (IVM) medium at three different concentrations (2.5, 5, and 10 mg/ml). Maturation rate, embryo cleavage, and blastocyst rate were recorded. Read More

View Article and Full-Text PDF

The osteogenic assessment of MTA-based endodontic sealers in an organotypic ex vivo bone development model.

J Endod 2021 Jun 11. Epub 2021 Jun 11.

BoneLab - Laboratory for Bone Metabolism and Regeneration, Faculty of Dental Medicine, U. Porto, Porto, Portugal; LAQV/REQUIMTE - U. Porto, Porto, Portugal.

Introduction: MTA-based sealers are endodontic materials with a widespread success in distinct clinical applications, potentially embracing a direct contact with the bone tissue. Bone response to these materials has been traditionally addressed in vitro. Nonetheless, translational data is limited by the absence of native cell-to-cell and cell-to-matrix interactions that hinder the representativeness of the analysis. Read More

View Article and Full-Text PDF

The miR-302c/transforming growth factor-β receptor type-2 axis modulates interleukin-1β-induced degenerative changes in osteoarthritic chondrocytes.

J Cell Commun Signal 2021 Jun 14. Epub 2021 Jun 14.

Department of Orthopedics, The Second Xiangya Hospital, Central South University, Changsha, People's Republic of China.

Chondrocyte production of catabolic and inflammatory mediators participating in extracellular matrix degradation has been regarded as a central event in osteoarthritis (OA) development. During OA pathogenesis, interleukin-1β (IL-1β) decreases the mRNA expression and protein levels of transforming growth factor-β receptor type-2 (TGFBR2), thus disrupting transforming growth factor-β signaling and promoting OA development. In the present study, we attempted to identify the differentially expressed genes in OA chondrocytes upon IL-1β treatment, investigate their specific roles in OA development, and reveal the underlying mechanism. Read More

View Article and Full-Text PDF

Glioblastoma cells potentiate the induction of the Th1-like profile in phosphoantigen-stimulated γδ T lymphocytes.

J Neurooncol 2021 Jun 14. Epub 2021 Jun 14.

Instituto de Medicina Experimental - CONICET - Academia Nacional de Medicina, Buenos Aires, Argentina.

Purpose: γδ T lymphocytes are non-conventional T cells that participate in protective immunity and tumor surveillance. In healthy humans, the main subset of circulating γδ T cells express the TCRVγ9Vδ2. This subset responds to non-peptide prenyl-pyrophosphate antigens such as (E)-4-hydroxy-3-methyl-but-enyl pyrophosphate (HMBPP). Read More

View Article and Full-Text PDF

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq.

J Vis Exp 2021 May 28(171). Epub 2021 May 28.

Department of Developmental and Cell Biology, University of California, Irvine;

Distilling and reporting large datasets, such as whole genome or transcriptome data, is often a daunting task. One way to break down results is to focus on one or more gene families that are significant to the organism and study. In this protocol, we outline bioinformatic steps to generate a phylogeny and to quantify the expression of genes of interest. Read More

View Article and Full-Text PDF

Engineering broad-spectrum resistance to cotton leaf curl disease by CRISPR-Cas9 based multiplex editing in plants.

GM Crops Food 2021 Jun 14:1-12. Epub 2021 Jun 14.

Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University Multan, Multan, Pakistan.

Advances in genome editing technologies have tremendous potential to address the limitations of classical resistance breeding. CRISPR-Cas9 based gene editing has been applied successfully in plants to tolerate virus infections. In this study, we successfully tested CRISPR-Cas9 system to counteract cotton leaf curl disease (CLCuD) caused by whitefly transmitted cotton leaf curl viruses (CLCuVs). Read More

View Article and Full-Text PDF

Chk1 suppression leads to a reduction in the enhanced radiation-induced invasive capability on breast cancer cells.

J Radiat Res 2021 Jun 14. Epub 2021 Jun 14.

Department of Radiation Oncology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.

Radiation therapy is generally effective for treating breast cancers. However, approximately 30% of patients with breast cancer experience occasional post-treatment local and distant metastasis. Low-dose (0. Read More

View Article and Full-Text PDF

Altered Genes and Biological Functions in Response to Severe Burns.

Biomed Res Int 2021 24;2021:8836243. Epub 2021 May 24.

Department of Plastic and Aesthetic Surgery, The Fifth Affiliated Hospital of Guangxi Medical University & The First People's Hospital of Nanning, NO.89, Qi-Xing Road, Nanning, Guangxi 530022, China.

Severe burns are acute wounds caused by local heat exposure, resulting in life-threatening systemic effects and poor survival. However, the specific molecular mechanisms remain unclear. First, we downloaded gene expression data related to severe burns from the GEO database (GSE19743, GSE37069, and GSE77791). Read More

View Article and Full-Text PDF

LINC02678 as a Novel Prognostic Marker Promotes Aggressive Non-small-cell Lung Cancer.

Front Cell Dev Biol 2021 28;9:686975. Epub 2021 May 28.

Department of Pathology, Harbin Medical University Cancer Hospital, Harbin, China.

Non-small-cell lung carcinoma (NSCLC) is considered to be a fatal disease and characterized by a poor prognosis. Long non-coding RNAs (lncRNAs) have been reported to act as biomarkers and therapeutic targets in solid tumors. However, the expression of lncRNAs and their clinical relevance in NSCLC remain undetermined. Read More

View Article and Full-Text PDF

TP53 Mutational Status-Based Genomic Signature for Prognosis and Predicting Therapeutic Response in Pancreatic Cancer.

Front Cell Dev Biol 2021 26;9:665265. Epub 2021 May 26.

Department of General Surgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

TP53 mutation is a critical driver mutation that affects the carcinogenesis and prognosis of patients with pancreatic cancer (PC). Currently, there is no driver mutation-derived signature based on TP53 mutational status for prognosis and predicting therapeutic response in PC. In the present study, we characterized the TP53 mutational phenotypes in multiple patient cohorts and developed a prognostic TP53-associated signature based on differentially expressed genes between PC samples with mutated TP53 and wild-type TP53. Read More

View Article and Full-Text PDF

Rapamycin Improves the Response of Effector and Memory CD8 T Cells Induced by Immunization With ASP2 of .

Front Cell Infect Microbiol 2021 25;11:676183. Epub 2021 May 25.

Molecular Immunology Laboratory, Center of Molecular and Cellular Therapy, Federal University of São Paulo (UNIFESP), São Paulo, Brazil.

Deficiency in memory formation and increased immunosenescence are pivotal features of infection proposed to play a role in parasite persistence and disease development. The vaccination protocol that consists in a prime with plasmid DNA followed by the boost with a deficient recombinant human adenovirus type 5, both carrying the ASP2 gene of , is a powerful strategy to elicit effector memory CD8 T-cells against this parasite. In virus infections, the inhibition of mTOR, a kinase involved in several biological processes, improves the response of memory CD8 T-cells. Read More

View Article and Full-Text PDF