Bioresour Technol 2021 Oct 12:126116. Epub 2021 Oct 12.
School of Civil Engineering, Chang'an University, The middle section of the south 2(nd) ring road, 710064, Xi'an, Shaanxi Province, China; Key Laboratory of Water Supply & Sewage Engineering, Ministry of Housing and Urban-rural Development, Chang'an University, The middle section of the south 2(nd) ring road, 710064, Xi'an, Shaanxi Province, China. Electronic address:
A heterotrophic nitrification- aerobic denitrification (HNAD) bacterium, Acinetobacter junii ZHG-1, was isolated, meanwhile, the optimal conditions for the strain were evaluated, moreover, the influence mechanism of the C/N ratio on the HNAD process was investigated from the perspective of electron transport and energy level. The increasing of C/N ratio enhanced the reduced/oxidized nicotinamide adenine dinucleotide (NADH/NAD) ratio, NADH concentration, electron transport system activity (ETSA), ATP content, as well as enzymes activities, consequently, the HNAD performance of the strain can be improved, however, when the C/N ratio was higher than 30, the activities of enzymes relating to the HNAD process and the ETSA had reached the maximum, which might limit the further improvement of the nitrogen removal with the increasing of C/N ratio. As the interaction between different biochemical reactions in HNAD process, more efforts should be devoted to the influent mechanism of different environmental factors on the HNAD process. Read More