541 results match your criteria endoxylanase produced

Glycosyl hydrolase 11 (xynA) gene with xylanase activity from thermophilic bacteria isolated from thermal springs.

Microb Cell Fact 2022 Apr 15;21(1):62. Epub 2022 Apr 15.

Biocatalysts Laboratory, Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, 641 003, India.

Background: Hemicellulose is one of the copious polymer in lignocellulosic biomass (LCB). It is primarily composed of xylan linked by β-1,4 glycosidic bonds. Xylanase preferentially cleaves the β-1,4-glycosidic bonds in the xylan backbone resulting in complete hydrolysis of the biomass. Read More

View Article and Full-Text PDF

A Fungal Versatile GH10 Endoxylanase and Its Glycosynthase Variant: Synthesis of Xylooligosaccharides and Glycosides of Bioactive Phenolic Compounds.

Int J Mol Sci 2022 Jan 26;23(3). Epub 2022 Jan 26.

Department of Microbial & Plan Biotechnology, Centro de Investigaciones Biológicas Margarita Salas, Spanish National Research Council (CSIC), C/Ramiro de Maeztu 9, 28040 Madrid, Spain.

The study of endoxylanases as catalysts to valorize hemicellulosic residues and to obtain glycosides with improved properties is a topic of great industrial interest. In this work, a GH10 β-1,4-endoxylanase (XynSOS), from the ascomycetous fungus , has been heterologously produced in , purified, and characterized. rXynSOS is a highly glycosylated monomeric enzyme of 53 kDa that contains a functional CBM1 domain and shows its optimal activity on azurine cross-linked (AZCL)-beechwood xylan at 70 °C and pH 5. Read More

View Article and Full-Text PDF
January 2022

Native Liquid Chromatography and Mass Spectrometry to Structurally and Functionally Characterize Endo-Xylanase Proteoforms.

Int J Mol Sci 2022 Jan 24;23(3). Epub 2022 Jan 24.

Center for Proteomics and Metabolomics, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands.

Xylanases are of great value in various industries, including paper, food, and biorefinery. Due to their biotechnological production, these enzymes can contain a variety of post-translational modifications, which may have a profound effect on protein function. Understanding the structure-function relationship can guide the development of products with optimal performance. Read More

View Article and Full-Text PDF
January 2022

Application of a recombinant GH10 endoxylanase from Thermoascus aurantiacus for xylooligosaccharide production from sugarcane bagasse and probiotic bacterial growth.

J Biotechnol 2022 Mar 10;347:1-8. Epub 2022 Feb 10.

Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista "Júlio de Mesquita Filho", São José do Rio Preto, São Paulo, Brazil. Electronic address:

Xylooligosaccharides (XOs) are a promising class of prebiotics capable of selectively stimulating the growth of the beneficial intestinal microbiota against intestinal pathogens. They can be obtained from xylan present in residual lignocellulosic material from agriculture. Thus, in this study we produced XOs by extracting xylan from sugarcane bagasse and hydrolyzing it using the GH10 xylanase from Thermoascus aurantiacus expressed by Pichia pastoris. Read More

View Article and Full-Text PDF

Optimized production of xylooligosaccharides from poplar: A biorefinery strategy with sequential acetic acid/sodium acetate hydrolysis followed by xylanase hydrolysis.

Bioresour Technol 2022 Mar 7;347:126683. Epub 2022 Jan 7.

Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China; Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, China; College of Forestry, Northwest A&F University, Yangling 712100, China. Electronic address:

The preparation of xylooligosaccharides (XOS) from lignocelluloses by organic acid hydrolysis has the advantages of high efficiency and simplicity, but reducing the production of by-products, especially xylose, is a prerequisite for commercial preparation of XOS using organic acid. In this work, to reduce the production of by-products, the acetic acid/sodium acetate conjugate system (AC/SA) was used to prepare XOS from poplar. Under the optimal conditions (0. Read More

View Article and Full-Text PDF

Microwave-assisted enzymatic hydrolysis to produce xylooligosaccharides from rice husk alkali-soluble arabinoxylan.

Sci Rep 2022 01 7;12(1):11. Epub 2022 Jan 7.

Department of Agro-Industry, Faculty of Agriculture Natural Resources and Environment, Naresuan University, Phitsanulok, 65000, Thailand.

The prebiotic properties of xylooligosaccharides (XOS) and arabino-xylooligosaccharides (AXOS) produced from rice husk (RH) using microwave treatment combined with enzymatic hydrolysis were evaluated. The RH was subjected to microwave pretreatment at 140, 160 and 180 °C for 5, 10 and 15 min to obtain crude arabinoxylan (AX). Increasing microwave pretreatment time increased sugar content. Read More

View Article and Full-Text PDF
January 2022

Paludibacter propionicigenes GH10 xylanase as a tool for enzymatic xylooligosaccharides production from heteroxylans.

Carbohydr Polym 2022 Jan 21;275:118684. Epub 2021 Sep 21.

Instituto de Física de São Carlos, Universidade de São Paulo, Avenida Trabalhador São-carlense 400, 13566-590 São Carlos, SP, Brazil. Electronic address:

Bioconversion of lignocellulosic biomass into value-added products relies on polysaccharides depolymerization by carbohydrate active enzymes. This work reports biochemical characterization of Paludibacter propionicigenes xylanase from GH10 (PpXyn10A) and its application for enzymatic xylooligosaccharides (XOS) production from commercial heteroxylans and liquor of hydrothermally pretreated corn cobs (PCC). PpXyn10A is tolerant to ethanol and NaCl, and releases xylobiose (X2) and xylotriose (X3) as the main hydrolytic products. Read More

View Article and Full-Text PDF
January 2022

A Novel Multifunctional Arabinofuranosidase/Endoxylanase/β-Xylosidase GH43 Enzyme from Paenibacillus curdlanolyticus B-6 and Its Synergistic Action To Produce Arabinose and Xylose from Cereal Arabinoxylan.

Appl Environ Microbiol 2021 11 6;87(24):e0173021. Epub 2021 Oct 6.

School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok, Thailand.

PcAxy43B is a modular protein comprising a catalytic domain of glycoside hydrolase family 43 (GH43), a family 6 carbohydrate-binding module (CBM6), and a family 36 carbohydrate-binding module (CBM36) and found to be a novel multifunctional xylanolytic enzyme from Paenibacillus curdlanolyticus B-6. This enzyme exhibited α-l-arabinofuranosidase, endoxylanase, and β-d-xylosidase activities. The α-l-arabinofuranosidase activity of PcAxy43B revealed a new property of GH43, via the release of both long-chain cereal arabinoxylan and short-chain arabinoxylooligosaccharide (AXOS), as well as release from both the C(O) and C(O) positions of AXOS, which is different from what has been seen for other arabinofuranosidases. Read More

View Article and Full-Text PDF
November 2021

Two-step acetic acid/sodium acetate and xylanase hydrolysis for xylooligosaccharides production from corncob.

Bioresour Technol 2021 Dec 20;342:125979. Epub 2021 Sep 20.

Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, PR China; Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, PR China; College of Forestry, Northwest A&F University, Yangling 712100, PR China. Electronic address:

At present, xylooligosaccharides (XOS) from corncob using acid-base conjugate system has not been reported. In this study, XOS production from corncob by two-step acetic acid/sodium acetate (AC/SA) conjugate system hydrolysis and xylanase hydrolysis was optimized, and monosaccharides were subsequently produced from corncob residues by cellulase hydrolysis. The XOS of 19. Read More

View Article and Full-Text PDF
December 2021

Effect of an endo-1,4-β-xylanase from wheat malt on water-unextractable arabinoxylan derived from wheat.

J Sci Food Agric 2022 Mar 5;102(5):1912-1918. Epub 2021 Oct 5.

College of Food Science and Engineering, Shandong Agricultural University, Tai'an, China.

Background: Non-starch polysaccharides in wheat are dominated by arabinoxylan (AX). Endo-1,4-β-xylanase (EC 3.2. Read More

View Article and Full-Text PDF

Feed endoxylanase type and dose affect arabinoxylan hydrolysis and fermentation in ageing broilers.

Anim Nutr 2021 Sep 3;7(3):787-800. Epub 2021 May 3.

Laboratory of Food Chemistry and Biochemistry, Department of Microbial and Molecular Systems (MS), KU Leuven, 3001, Leuven, Belgium.

Despite the general use of endoxylanases in poultry feed to improve broiler performance, the abundance of different endoxylanase products and the variable response to their application in the field prevent a clear understanding of endoxylanase functionality in vivo. To gain insight into this functionality, we investigated the impact of endoxylanase type (Belfeed from versus Econase XT from ) and dose (10, 100, 1,000 mg/kg) in combination with broiler age on arabinoxylan (AX) hydrolysis and fermentation in broilers (Ross 308) fed a wheat-soy based diet. In a digestibility trial and a performance trial, a total of 1,057 one-day-old chicks received the control diet or 1 of the 6 endoxylanase supplemented wheat-soy based diets with, respectively, 5 replicate cages and 8 replicate pens per dietary treatment per trial. Read More

View Article and Full-Text PDF
September 2021

Endo-xylanases from Cohnella sp. AR92 aimed at xylan and arabinoxylan conversion into value-added products.

Appl Microbiol Biotechnol 2021 Sep 30;105(18):6759-6778. Epub 2021 Aug 30.

Planta Piloto de Procesos Industriales Microbiológicos PROIMI-CONICET, Av. Belgrano Y Pasaje Caseros, T4001 MVB, 4000, San Miguel de Tucumán, Argentina.

The genus Cohnella belongs to a group of Gram-positive endospore-forming bacteria within the Paenibacillaceae family. Although most species were described as xylanolytic bacteria, the literature still lacks some key information regarding their repertoire of xylan-degrading enzymes. The whole genome sequence of an isolated xylan-degrading bacterium Cohnella sp. Read More

View Article and Full-Text PDF
September 2021

Fungal glycosyl hydrolases for sustainable plant biomass valorization: Talaromyces amestolkiae as a model fungus.

Int Microbiol 2021 Nov 21;24(4):545-558. Epub 2021 Aug 21.

Centro de Investigaciones Biológicas Margarita Salas (CIB-CSIC), C/ Ramiro de Maeztu 9, 28022, Madrid, Spain.

As the main decomposers and recyclers in nature, fungi secrete complex mixtures of extracellular enzymes for degradation of plant biomass, which is essential for mobilization of the organic carbon fixed by the photosynthesis in vegetal cells. Biotechnology can emulate the closed natural biological cycles, using lignocellulosic biomass as a renewable resource and lignocellulolytic fungal enzymes as catalysts to sustainably produce consumer goods. Cellulose and hemicellulose are the major polysaccharides on Earth, and the main enzymes involved in their hydrolytic depolymerization are cellulases (endoglucanases, cellobiohydrolases, and β-glucosidases) and hemicellulases (mainly endoxylanases and β-xylosidases). Read More

View Article and Full-Text PDF
November 2021

Paecilomyces variotii xylanase production, purification and characterization with antioxidant xylo-oligosaccharides production.

Sci Rep 2021 08 13;11(1):16468. Epub 2021 Aug 13.

Department of Industrial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt.

Paecilomyces variotii xylanase was, produced in stirred tank bioreactor with yield of 760 U/mL and purified using 70% ammonium sulfate precipitation and ultra-filtration causing 3.29-fold purification with 34.47% activity recovery. Read More

View Article and Full-Text PDF

Non-Specific GH30_7 Endo-β-1,4-xylanase from .

Molecules 2021 Jul 30;26(15). Epub 2021 Jul 30.

Institute of Chemistry, Slovak Academy of Sciences, Dúbravská Cesta 9, SK-845 38 Bratislava, Slovakia.

This study describes the catalytic properties of a GH30_7 xylanase produced by the fungus . The enzyme is an ando-β-1,4-xylanase, showing similar specific activity towards glucuronoxylan, arabinoxylan, and rhodymenan (linear β-1,3-β-1,4-xylan). The heteroxylans are hydrolyzed to a mixture of linear as well as branched β-1,4-xylooligosaccharides that are shorter than the products generated by GH10 and GH11 xylanases. Read More

View Article and Full-Text PDF

The Jo-In protein welding system is a relevant tool to create CBM-containing plant cell wall degrading enzymes.

N Biotechnol 2021 Nov 2;65:31-41. Epub 2021 Aug 2.

TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France. Electronic address:

Irrespective of their biological origin, most proteins are composed of several elementary domains connected by linkers. These domains are either functionally independent units, or part of larger multidomain structures whose functions are defined by their spatial proximity. Carbohydrate-degrading enzymes provide examples of a range of multidomain structures, in which catalytic protein domains are frequently appended to one or more non-catalytic carbohydrate-binding modules which specifically bind to carbohydrate motifs. Read More

View Article and Full-Text PDF
November 2021

Factors Affecting the Catalytic Efficiency and Synergism of Xylanase and Cellulase During Enzymatic Hydrolysis of Birch Wood.

Appl Biochem Biotechnol 2021 Nov 10;193(11):3469-3482. Epub 2021 Jul 10.

Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, No. 2 Energy Road, Tianhe District, Guangzhou, 510640, People's Republic of China.

Understanding factors that affect the catalytic efficiency and synergism of enzymes is helpful to enhance the process of bioconversion. In this study, birch wood (BW) was sequentially treated by delignification (DL), deacetylation (DA), and decrystallization (DC) treatments. The physiochemical structures of treated BW were characterized. Read More

View Article and Full-Text PDF
November 2021

Eco-friendly bleaching of sugarcane bagasse with crude xylanase and pectinase enzymes to reduce the bleaching effluent toxicity.

Environ Sci Pollut Res Int 2021 Aug 3;28(31):42990-42998. Epub 2021 Jul 3.

Department of Biotechnology, Kurukshetra University, Kurukshetra, India.

Bio-bleaching effect on bagasse pulp using xylano-pectinolytic enzymes produced by a bacterial species was studied in order to evaluate the potential of these enzymes in paper industry. In this study, action of enzymes was maximum with xylanase/pectinase dose 7/1.75 IU/g, pulp consistency 1:12. Read More

View Article and Full-Text PDF

Purification and characterization of a highly-stable fungal xylanase from cultivated on palm wastes through combined solid-state and submerged fermentation.

Prep Biochem Biotechnol 2022 1;52(3):311-317. Epub 2021 Jul 1.

Program of Biotechnology, Faculty of Agro-Industry, Center of Excellence in Innovative Biotechnology for Sustainable Utilization of Bioresources, Prince of Songkla University, Songkhla, Thailand.

Fungal xylanase was produced from lignocellulosic palm wastes through combined solid-state fermentation (SSF) and submerged fermentation (SmF) by TSIP9 in a helical-impeller equipped bioreactor. The combined SSF-SmF promoted the xylanase production by 15 and 70% higher than SSF and SmF, respectively. Sequential purification yielded 7. Read More

View Article and Full-Text PDF

Biochemical properties of cellulolytic and xylanolytic enzymes from and their utility in bioethanol production using rice straw.

Prep Biochem Biotechnol 2022 19;52(2):197-209. Epub 2021 May 19.

Department of Physics, RPS Degree College, Mahendergarh, India.

Production of cellulolytic and xylanolytic enzymes by was enhanced using response surface methodology in solid-state fermentation (SSF) using wheat straw and cotton oil cake. Cellulolytic and xylanolytic enzymes were partially purified by ammonium sulfate precipitation followed by ion exchange and gel filtration chromatographic techniques. Xylanase of is neutral xylanase displaying optimal activity at 60 °C with and values of 0. Read More

View Article and Full-Text PDF

Evaluation of nutraceutical application of xylooligosaccharide enzymatically produced from cauliflower stalk for its value addition through a sustainable approach.

Food Funct 2021 Jun;12(12):5501-5523

School of Community Science and Technology, Indian Institute of Engineering Science and Technology, Shibpur, India.

There is increasing attention on the exploration of waste feedstocks as economically viable substrates for the production of prebiotic oligosaccharides, especially xylooligosaccharides, as excellent candidates for the maintenance and promotion of gut microbiota. XOS, an emerging prebiotic that has several functional attributes and beneficial health effects, is mainly produced by different processes, especially enzymatic hydrolysis through the valorisation of xylan enriched lignocellulosic materials. The present study deals with the enzymatic production of xylooligosaccharide (XOS) from xylan rich cauliflower stalk, a novel source. Read More

View Article and Full-Text PDF

Cloning and Heterologous Expression of a Novel Xylanase Gene TAX1 from Trichoderma atroviride and Its Application in the Deconstruction of Corn Stover.

Appl Biochem Biotechnol 2021 Oct 10;193(10):3029-3044. Epub 2021 May 10.

Northeast Agricultural University, Harbin, 150030, People's Republic of China.

Xylanase plays a vital role in the efficient utilization of xylan, which accounts for up to 30% of plant dry matter. However, the production cost of xylanase remains high, and the enzymatic characteristics of xylanases of most microorganisms are not suitable for industrial production. Therefore, it is of great significance to discover and develop new and efficient xylanases. Read More

View Article and Full-Text PDF
October 2021

Method for the Production and Purification of Plant Immuno-Active Xylanase from .

Int J Mol Sci 2021 Apr 19;22(8). Epub 2021 Apr 19.

Department of Plant Pathology and Weed Research, Plant Protection Institute, Agricultural Research Organization, Volcani Institute, Rishon LeZion 50250, Israel.

Plants lack a circulating adaptive immune system to protect themselves against pathogens. Therefore, they have evolved an innate immune system based upon complicated and efficient defense mechanisms, either constitutive or inducible. Plant defense responses are triggered by elicitors such as microbe-associated molecular patterns (MAMPs). Read More

View Article and Full-Text PDF

A biorefinery approach for enzymatic complex production for the synthesis of xylooligosaccharides from sugarcane bagasse.

Bioresour Technol 2021 Aug 16;333:125174. Epub 2021 Apr 16.

Bioprocess Engineering and Biotechnology Department, Federal University of Paraná, Brazil, Centro Politécnico, CP 19011, Curitiba-PR 81531-980, Brazil.

The use of low-cost feedstock for enzyme production is an environmental and economic solution. Sugarcane bagasse and soybean meal are employed in this study for optimised xylanase production with the concomitant synthesis of proteases. The enzymatic complex is produced by submerged fermentation by Aspergillus niger. Read More

View Article and Full-Text PDF

Structure-based substrate specificity analysis of GH11 xylanase from Streptomyces olivaceoviridis E-86.

Appl Microbiol Biotechnol 2021 Mar 10;105(5):1943-1952. Epub 2021 Feb 10.

Department of Subtropical Biochemistry and Biotechnology, Faculty of Agriculture, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa, 903-0213, Japan.

Although many xylanases have been studied, many of the characteristics of xylanases toward branches in xylan remain unclear. In this study, the substrate specificity of a GH11 xylanase from Streptomyces olivaceoviridis E-86 (SoXyn11B) was elucidated based on its three-dimensional structure. Subsite mapping suggests that SoXyn11B has seven subsites (four subsites on the - side and three subsites on the + side), and it is one longer than the GH10 xylanase from S. Read More

View Article and Full-Text PDF

Hydrolysis pattern analysis of xylem tissues of woody plants pretreated with hydrogen peroxide and acetic acid: rapid saccharification of softwood for economical bioconversion.

Biotechnol Biofuels 2021 Feb 6;14(1):37. Epub 2021 Feb 6.

Bio-Energy Research Center, Chonnam National University, Gwangju, 500-757, Republic of Korea.

Background: Woody plants with high glucose content are alternative bioresources for the production of biofuels and biochemicals. Various pretreatment methods may be used to reduce the effects of retardation factors such as lignin interference and cellulose structural recalcitrance on the degradation of the lignocellulose material of woody plants.

Results: A hydrogen peroxide-acetic acid (HPAC) pretreatment was used to reduce the lignin content of several types of woody plants, and the effect of the cellulose structural recalcitrance on the enzymatic hydrolysis was analyzed. Read More

View Article and Full-Text PDF
February 2021

Proteomic analysis of secretomes from sp. AR03: characterization of enzymatic cocktails active on complex carbohydrates for xylooligosaccharides production.

Prep Biochem Biotechnol 2021 13;51(9):871-880. Epub 2021 Jan 13.

Planta Piloto de Procesos Industriales Microbiológicos (PROIMI), CONICET, Tucumán, Argentina.

sp. AR03 have been described as an important producer of carbohydrate-active enzymes (CAZymes) when growing in a peptone-based medium supplemented with simple sugars and/or carboxymethyl cellulose (CMC) as carbon sources. This work aimed to identify the extracellular enzymatic cocktails through shotgun proteomics. Read More

View Article and Full-Text PDF
January 2022

Effect of N-terminal modification on the mode of action between the Xyn11A and Xylotetraose.

Int J Biol Macromol 2021 Feb 24;170:240-247. Epub 2020 Dec 24.

Department of Microbiology, Science Laboratory Building, Faculty of Science, King Mongkut's University of Technology Thonburi (KMUTT), Bangkok 10140, Thailand. Electronic address:

The purpose of this study was to gain an insight into the effects of mutation-induced binding pocket tilting of the Xyn11A xylanase from Bacillus firmus K-1 in producing a unique hydrolysis characteristic. In this study, the wildtype Xyn11A and its K40L mutant were compared for their hydrolysis patterns on beechwood xylan and xylooligosaccharides of sizes 2 to 6. According to our thin-layer chromatography experiment, the K40L mutant produced a larger amount of xylotetraose leftover than the wildtype. Read More

View Article and Full-Text PDF
February 2021

Xylooligosaccharides from lignocellulosic biomass: A comprehensive review.

Carbohydr Polym 2021 Jan 22;251:117118. Epub 2020 Sep 22.

Centre for Biotechnology and Bioengineering, Department of Chemical Engineering, Biotechnology and Materials, University of Chile, Beauchef 851, Santiago, Zipcode 8370448, Chile. Electronic address:

Xylooligosaccharides (XOS), produced from lignocellulosic biomass (LCB), are short-chain polymers with prebiotic activity which, in the last few decades, have gained commercial interest due to their potential application as ingredients for the nutraceutical industry. This article reviews relevant topics to consider when researching XOS productive processes, such as the selection of raw materials and strategies for XOS production, purification, characterisation, quantification and evaluation of the prebiotic effects. With regard to the production approach, this article focuses on LCB pre-treatments and the enzymatic hydrolysis of xylan, exploring the reported alternatives and enzymes. Read More

View Article and Full-Text PDF
January 2021

Enhancement of catalytic performance of a metagenome-derived thermophilic oligosaccharide-specific xylanase by binding module removal and random mutagenesis.

J Biosci Bioeng 2021 Jan 14;131(1):13-19. Epub 2020 Oct 14.

Enzyme Technology Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Road, Khlong Luang, Pathumthani 12120, Thailand. Electronic address:

Xylo-oligosaccharide (XO) is a promising pre-biotic with applications in food, feed and healthcare products. XO can be produced by enzymatic digestion of xylan with xylanase. In this study, we aimed to improve the biochemical properties relevant to catalysis and kinetics of X11, a thermophilic glycosyl hydrolase (GH) family 11 endo-β-1,4-xylanase derived from a metagenomic library isolated from sugarcane bagasse, under high-temperature conditions preferred for XO synthesis. Read More

View Article and Full-Text PDF
January 2021