Search Our Scientific Publications & Authors

Microvasc Res 2021 Apr 9:104165. Epub 2021 Apr 9.

Department of Physiology, KU Open Innovation Center, Research Institute of Medical Science, Konkuk University School of Medicine, Chungju, South Korea. Electronic address:

Phototherapy has been tried for treating cardiovascular diseases. In particular, ultraviolet and blue visible lights were suggested to be useful due to their nitric oxide (NO)-production ability in the skin. However, the effects of blue light on the arterial contractility is controversial. Read More

Microvasc Res 2021 Apr 9:104168. Epub 2021 Apr 9.

Gomel State Medical University, 5 Lange str., Gomel, BY-246000, Belarus.

Endothelial cells, due to heterogeneity in the cell structure, can potentially form an inhomogeneous on structural and mechanical properties of the inner layer of the capillaries. Using quantitative nanomechanical mapping mode of atomic force microscopy, the parameters of the structural, elastic, and adhesive properties of the cell surface for living and glutaraldehyde-fixed human umbilical vein endothelial cells were studied. A significant difference in the studied parameters for three cell surface zones (peripheral, perinuclear, and nuclear zones) was established. Read More

Mol Pharm 2021 Apr 12. Epub 2021 Apr 12.

Department of Pharmaceutical Sciences, University of Basel, Basel 4056, Switzerland.

Recently, a lipopeptide derived from the hepatitis B virus (HBV) large surface protein has been developed as an HBV entry inhibitor. This lipopeptide, called MyrcludexB (MyrB), selectively binds to the sodium taurocholate cotransporting polypeptide (NTCP) on the basolateral membrane of hepatocytes. Here, the feasibility of coupling therapeutic enzymes to MyrB was investigated for the development of enzyme delivery strategies. Read More

Clin Exp Pharmacol Physiol 2021 Apr 12. Epub 2021 Apr 12.

Department of Cardiology, Fourth Affiliated Hospital of Harbin Medical University, Harbin, 150001, China.

Circular RNAs (circRNAs) are widely expressed in mammals and act as regulatory targets in the atherogenesis. The objective of this study was to research the biological role and molecular mechanism of circ_0093887 in oxidized low-density lipoprotein (ox-LDL)-induced AS of human aortic endothelial cells (HAECs). Cell viability detection was performed by CCK-8 assay. Read More

Neurotherapeutics 2021 Apr 12. Epub 2021 Apr 12.

Institute of Clinical Pharmacology, Medical Faculty, Goethe-University, Frankfurt, Germany.

Depletion of the enzyme cofactor, tetrahydrobiopterin (BH4), in T-cells was shown to prevent their proliferation upon receptor stimulation in models of allergic inflammation in mice, suggesting that BH4 drives autoimmunity. Hence, the clinically available BH4 drug (sapropterin) might increase the risk of autoimmune diseases. The present study assessed the implications for multiple sclerosis (MS) as an exemplary CNS autoimmune disease. Read More

Angiogenesis 2021 Apr 12. Epub 2021 Apr 12.

Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA, 22908, USA.

There are two vascular networks in mammals that coordinately function as the main supply and drainage systems of the body. The blood vasculature carries oxygen, nutrients, circulating cells, and soluble factors to and from every tissue. The lymphatic vasculature maintains interstitial fluid homeostasis, transports hematopoietic cells for immune surveillance, and absorbs fat from the gastrointestinal tract. Read More

Biosci Rep 2021 Apr 12. Epub 2021 Apr 12.

Qiqihar Medical University, Qiqihar, China.

Interleukin-8 (IL-8) promotes cell homing and angiogenesis, but its effects on activating human bone marrow mesenchymal stem cells (BMSCs) and promoting angiogenesis are unclear. We used bioinformatics to predict these processes. In vitro, BMSCs were stimulated in a high-glucose (HG) environment with 50 μg/mL or 100 μg/mL IL-8 were used as the IL-8 group. Read More

J Neuromuscul Dis 2021 Apr 2. Epub 2021 Apr 2.

Institut NeuroMyoGène, Université Claude Bernard Lyon 1, CNRS UMR, INSERM, Université Lyon, Villeurbanne, France.

Background: The mdx-C57/B6 mouse model does not show the clinical signs of Duchenne muscular dystrophy (DMD), although muscles exhibit hallmarks of permanent regeneration and alterations in muscle function. The DMDmdx4Cv strain exhibits very few revertant dystrophin positive myofibers, making that model suitable for studies on gene and cell therapies.

Objective: The study appraises the histological evolution of the Tibialis Anterior muscle of WT and DMD mdx4Cv mutant from 1 to 24 months. Read More

Elife 2021 Apr 12;10. Epub 2021 Apr 12.

Medicine/Immunology and Microbiology, University of Colorado Anschutz Medical Campus, Aurora, United States.

The detection of foreign antigens has relied on fluorescent conjugation or indirect read-outs such as antigen presentation. In our studies, we found that these widely used techniques had several technical limitations that have precluded a complete picture of antigen trafficking or retention across lymph node cell types. To address these limitations, we developed a 'molecular tracking device' to follow the distribution, acquisition, and retention of antigen in the lymph node. Read More

Virulence 2021 Dec;12(1):1186-1198

Institute of Medical Microbiology, Jena University Hospital, Jena, Germany.

A large proportion of clinical isolates that carry an inactive Agr system are associated with persistent infection that is difficult to treat. Once is inside the bloodstream, it can cross the endothelial barrier and invade almost every organ in the human body. Endothelial cells can either be lysed by this pathogen or they serve as a niche for its intracellular long-term survival. Read More

Autophagy 2021 Apr 12:1-20. Epub 2021 Apr 12.

College of Pharmacy, Chongqing Medical University, Chongqing, People's Republic of China.

Zinc oxide nanoparticles (ZnONPs) hold great promise for biomedical applications. Previous studies have revealed that ZnONPs exposure can induce toxicity in endothelial cells, but the underlying mechanisms have not been fully elucidated. In this study, we report that ZnONPs can induce ferroptosis of both HUVECs and EA. Read More

Nat Prod Res 2021 Apr 11:1-7. Epub 2021 Apr 11.

Department of Orthopaedics, The Second People's Hospital of Yibin, Sichuan, P.R. China.

Two novel icetexane diterpenes were isolated from Maxim., namely Salprzesides A () and B (), together with two known abietane-type diterpenes respectively identified as sahandinone () and miltirone (). The structures of isolated compounds were determined by UV, IR, HR-ESI-MS, 1D and 2D NMR analysis. Read More

AJOG Glob Rep 2021 Feb 27;1(1):100001. Epub 2021 Jan 27.

Department of Virology and Biotechnology Research, Gorgas Memorial Institute for Health Studies, Panama City, Republic of Panama (Ms Gaitán, Ms Chen-Germán, Ms Araúz, Saenz and Dr López-Vergès).

There is limited evidence regarding severe acute respiratory syndrome coronavirus 2 infection in the placenta of pregnant women who tested positive, and if this could be a route for vertical transmission of the virus in utero. We present the cases of 2 pregnant women in their third trimester who were admitted for delivery by cesarean delivery and who, through universal screening, tested positive for coronavirus disease 2019. The maternal and fetal sides of the placenta were sectioned from both patients for viral analysis. Read More

Bioact Mater 2021 Oct 21;6(10):3515-3527. Epub 2021 Mar 21.

Department of Biomedical Engineering and Hubei Province Key Laboratory of Allergy and Immune Related Diseases, School of Basic Medical Sciences, Wuhan University, Wuhan, 430071, China.

Peripheral nerve regeneration requires stepwise and well-organized establishment of microenvironment. Since local delivery of VEGF-A in peripheral nerve repair is expected to promote angiogenesis in the microenvironment and Schwann cells (SCs) play critical role in nerve repair, combination of VEGF and Schwann cells may lead to efficient peripheral nerve regeneration. VEGF-A overexpressing Schwann cells were established and loaded into the inner wall of hydroxyethyl cellulose/soy protein isolate/polyaniline sponge (HSPS) conduits. Read More

Ann Transl Med 2021 Mar;9(5):394

Department of Oromaxillofacial Head and Neck Oncology, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Though infantile hemangioma (IH) is a common benign vascular tumor, its pathogenesis remains unclear. This study explored the function of hemangioma-derived stem cells (HemSCs) derived exosomes, which exerted an intercellular effect on hemangioma-derived endothelial cells (HemECs).

Methods: First, HemSCs and HemECs were extracted and cultured. Read More

Front Cardiovasc Med 2021 26;8:613400. Epub 2021 Mar 26.

Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA, United States.

Intravascular optical coherence tomography (IVOCT) that produces images with 10 μm resolution has emerged as a significant technology for evaluating coronary architectural morphology. Yet, many features that are relevant to coronary plaque pathogenesis can only be seen at the cellular level. This issue has motivated the development of a next-generation form of OCT imaging that offers higher resolution. Read More

Front Mol Biosci 2021 26;8:636587. Epub 2021 Mar 26.

Department of Medical Sciences, Molecular Biotechnology Center, University of Torino, Turin, Italy.

Extracellular vesicles (EVs) have emerged in the last decades as a cell-to-cell communication mechanism. One of their mechanism of action is the direct delivery of their cargo, composed of bioactive molecules to target cells. Different methods (direct electroporation, cell transfection, chemical transfection) were developed to vehicle therapeutic molecules through EVs. Read More

Front Med (Lausanne) 2021 24;8:659013. Epub 2021 Mar 24.

Université de Paris, Paris Cardiovascular Center, Inserm, Paris, France.

Diabetes is the main cause of renal failure worldwide. Complications of the kidney micro-and macro-circulation are common in diabetic patients, leading to proteinuria and can progress to end-stage renal disease. Across the complex interplays aggravating diabetes kidney disease progression, lesions of the glomerular filtration barrier appear crucial. Read More

Front Cell Dev Biol 2021 25;9:660571. Epub 2021 Mar 25.

Department of Breast Surgery, Hainan General Hospital, Hainan Medical University, Haikou, China.

Electrospun nanofiber is an attractive biomaterial for skin tissue engineering because it mimics the natural fibrous extracellular matrix structure and creates a physical structure suitable for skin tissue regeneration. However, endowing the nanofibrous membranes with antibacterial and angiogenesis functions needs to be explored. In the current study, we aimed to fabricate gelatin/polycaprolactone (GT/PCL) (GT/PCL-Ag-Mg) nanofibers loaded with silver (Ag) and magnesium (Mg) ions for antibacterial activity and pro-angiogenesis function for wound repair. Read More

Front Cell Dev Biol 2021 25;9:646967. Epub 2021 Mar 25.

The First School of Clinical Medicine, Southern Medical University, Guangzhou, China.

The management of diabetic wounds is a therapeutic challenge in clinical settings. Current tissue engineering strategies for diabetic wound healing are insufficient, owing to the lack of an appropriate scaffold that can load a large number of stem cells and induce the interaction of stem cells to form granulation tissue. Herein we fabricated a book-shaped decellularized dermal matrix (BDDM), which shows a high resemblance to native dermal tissue in terms of its histology, microstructure, and ingredients, is non-cytotoxic and low-immunogenic, and allows adipose-derived stromal cell (ASC) attachment and proliferation. Read More

Front Cell Dev Biol 2021 26;9:641792. Epub 2021 Mar 26.

William J. von Liebig Center for Transplantation and Clinical Regeneration, Mayo Clinic, Rochester, MN, United States.

Mesenchymal stem/stromal cells (MSCs) have been investigated extensively for their immunotherapeutic and regenerative properties, which may differ by cell source. In MSCs harvested from donors matched for sex, age, and body mass index, we compared the proliferative and migration functions of liver-derived MSCs (L-MSCs) and adipose tissue-derived MSCs (A-MSCs) ( = 6 donors each). Cellular senescence was evaluated by senescence-associated beta-galactosidase enzyme activity and expression of senescence-associated secretory phenotype (SASP) factors using real-time quantitative polymerase chain and by western blot assay. Read More

Front Cell Dev Biol 2021 25;9:637248. Epub 2021 Mar 25.

Department of Neonatology, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Premature infants have a high risk of bronchopulmonary dysplasia (BPD), which is characterized by abnormal development of alveoli and pulmonary vessels. Exosomes and exosomal miRNAs (EXO-miRNAs) from bronchoalveolar lavage fluid are involved in the development of BPD and might serve as predictive biomarkers for BPD. However, the roles of exosomes and EXO-miRNAs from umbilical cord blood of BPD infants in regulating angiogenesis are yet to be elucidated. Read More

J Adv Res 2021 Mar 15;29:23-32. Epub 2020 Sep 15.

Department of Ion Channel Pharmacology, School of Pharmacy, China Medical University, Shenyang 110122, China.

Introduction: Ca-activated Cl channel TMEM16A is expressed in endothelial cells, and contributes to many diseases such as hypertension, blood-brain barrier dysfunction, and pulmonary hypertension. It remains unclear whether TMEM16A regulates endothelial angiogenesis, which participates in many physiological and pathological processes. Cholesterol regulates many ion channels including TMEM16A, and high cholesterol levels contribute to endothelial dysfunction. Read More

Clin Kidney J 2021 Apr 6;14(4):1055-1066. Epub 2020 Nov 6.

Josep Carreras Leukaemia Research Institute, Hospital Clinic/University of Barcelona Campus, Barcelona, Spain.

When faced with microangiopathic haemolytic anaemia, thrombocytopenia and organ dysfunction, clinicians should suspect thrombotic microangiopathy (TMA). The endothelial damage that leads to this histological lesion can be triggered by several conditions or diseases, hindering an early diagnosis and aetiological treatment. However, due to systemic involvement in TMA and its low incidence, an accurate early diagnosis is often troublesome. Read More

Am J Transl Res 2021 15;13(3):1710-1716. Epub 2021 Mar 15.

Department of Nephrology, China-Japan Friendship Hospital Beijing 100029, China.

Objective: To explore the relationship between neutrophil-lymphocyte ratio (NLR) and early renal fibrosis and renal prognosis in patients with lupus nephritis.

Methods: A total of 186 patients with lupus nephritis admitted to our hospital were enrolled and grouped according to the standard of "NLR=3.175". Read More

Am J Transl Res 2021 15;13(3):1505-1515. Epub 2021 Mar 15.

Department of Optometry Center, Tianjin Eye Hospital Tianjin 300022, China.

This study was aimed to determine the role of has-miR-155 and E2F2 on corneal endothelial cells. Real-time quantitative PCR and Western blot assays were carried out to determine the levels of has-miR-155 and E2F2, and Flow cytometry assay was conducted to detect cell cycle. In addition, Targetscan7. Read More

Am J Transl Res 2021 15;13(3):1432-1444. Epub 2021 Mar 15.

Department of Thyroid Surgery, The Affiliated Hospital of Southwest Medical University Luzhou 646000, China.

Sepsis-induced myocardial dysfunction (SIMD) is one of the leading causes of death in sepsis. We hypothesized that exosomes released from ECs exposed to bacterial lipopolysaccharides (LPS) have some regulatory effect on cardiomyocytes (CMs). In this study, cultured rat ECs were exposed to 0. Read More

Am J Transl Res 2021 15;13(3):1006-1021. Epub 2021 Mar 15.

Department of Obstetrics, Dongying District People's Hospital Dongying 257000, Shandong, China.

The present study aimed to explore the role of kelch-like ECH-associated protein-1 (Keap1)/Nuclear factor erythroid 2-related factor 2 (Nrf-2) signaling pathway in regulating heme oxygenase-1 (HO-1) expression in adverse outcomes of preeclampsia (PE). Adult Wistar rats, HTR-8/SVneo and hESC cells were used for models and , respectively. Inhibition of Nrf-2 could slightly reduce the elevation of systolic blood pressure (SBP) and urinary protein in PE rats. Read More

Front Genet 2021 24;12:632164. Epub 2021 Mar 24.

Department of Cardiology, Minhang Hospital, Fudan University, Shanghai, China.

Circular RNAs (circRNAs) are novel non-coding RNAs, which show abnormal expression in several diseases, such as atherosclerosis (AS). The purpose of the present study was to reveal the association between hsa_circ_0004543 and AS. In the present study, hsa_circ_0004543 was overexpressed in human umbilical vein endothelial cells (HUVECs) induced by oxidized low-density lipoprotein (oxLDL). Read More

Front Immunol 2021 26;12:632709. Epub 2021 Mar 26.

Division of Hematology, Oncology and Transplantation, Department of Medicine, University of Minnesota, Minneapolis, MN, United States.

Recent evidence indicates that hemolysis in sickle cell disease (SCD) promotes inflammation innate immune signaling through toll-like receptor 4 (TLR4). Free heme released by hemolyzed red blood cells can bind to myeloid differentiation factor-2 (MD-2) and activate TLR4 pro-inflammatory signaling on endothelium to promote vaso-occlusion and acute chest syndrome in murine models of SCD. MD-2 is co-expressed with TLR4 on cell membranes, but in inflammatory conditions, soluble MD-2 (sMD-2) is elevated in plasma. Read More