268,787 results match your criteria endothelial cell


Biocompatible Polyurethane Conduit Grafted with Vascular Endothelial Growth Factor-loaded Hydrogel Repairs the Peripheral Nerve Defect in Rats.

Macromol Biosci 2021 Dec 4:e2100397. Epub 2021 Dec 4.

Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, 200011, China.

Artificial nerve guidance conduits (NGCs) can be potentially used to address the problems of peripheral nerve defects. The biomaterial polyurethane (PU) has already been used to construct NGCs. However, the use of a combination of PU-based NGCs and other bioactive cues, such as extracellular matrix proteins and growth factors, has not been reported yet. Read More

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December 2021

Pathogenesis of COVID-19 described through the lens of an undersulfated and degraded epithelial and endothelial glycocalyx.

FASEB J 2022 Jan;36(1):e22052

School of Life Sciences, University of KwaZulu-Natal, Durban, South Africa.

The glycocalyx surrounds every eukaryotic cell and is a complex mesh of proteins and carbohydrates. It consists of proteoglycans with glycosaminoglycan side chains, which are highly sulfated under normal physiological conditions. The degree of sulfation and the position of the sulfate groups mainly determine biological function. Read More

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January 2022

Graft survival of Descemet membrane endothelial keratoplasty (DMEK) in corneal endothelial decompensation after glaucoma surgery.

Graefes Arch Clin Exp Ophthalmol 2021 Dec 4. Epub 2021 Dec 4.

Department of Ophthalmology, Faculty of Medicine and University Hospital Cologne, University of Cologne, Cologne, Germany.

Purpose: This study aims to assess the results, rebubbling rate, and graft survival after Descemet membrane endothelial keratoplasty (DMEK) with regard to the number and type of previous glaucoma surgeries.

Methods: This is a clinical retrospective review of 1845 consecutive DMEK surgeries between 07/2011 and 08/2017 at the Department of Ophthalmology, University of Cologne. Sixty-six eyes were included: group 1 (eyes with previous glaucoma drainage devices (GDD); n = 27) and group 2 (eyes with previous trabeculectomy (TE); n = 39). Read More

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December 2021

Design, synthesis, anticancer, and docking of some S- and/or N-heterocyclic derivatives as VEGFR-2 inhibitors.

Arch Pharm (Weinheim) 2021 Dec 3:e2100237. Epub 2021 Dec 3.

Department of Chemistry, Al-Azhar University (Girls Branch), Cairo, Egypt.

Novel heterocyclic derivatives (4-22) were designed, synthesized, and evaluated against hepatocellular carcinoma type (HepG2) and breast cancer (MCF-7) cells, targeting the VEGFR-2 enzyme. Compounds 18, 10, 13, 11, and 14 were found to be the most potent derivatives against both the HepG2 and MCF-7 cancer cell lines, with GI  = 2.11, 2. Read More

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December 2021

Targeting VEGFR-2 by new quinoxaline derivatives: Design, synthesis, antiproliferative assay, apoptosis induction, and in silico studies.

Arch Pharm (Weinheim) 2021 Dec 3:e2100359. Epub 2021 Dec 3.

Pharmaceutical Medicinal Chemistry & Drug Design Department, Faculty of Pharmacy (Boys), Al-Azhar University, Nasr, Egypt.

Twelve new triazolo[4,3-a]quinoxaline-based compounds are reported as anticancer agents with potential effects against vascular endothelial growth factor receptor-2 (VEGFR-2), using sorafenib as a reference molecule. With sorafenib as the positive control, the antiproliferative effects of the synthesized compounds against MCF-7 and HepG2 cells, as well as their VEGFR-2-inhibitory activities, were assessed. The most powerful VEGFR-2 inhibitor was compound 14a, which had an IC value of 3. Read More

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December 2021

Single-cell analysis of salt-induced hypertensive mouse aortae reveals cellular heterogeneity and state changes.

Exp Mol Med 2021 Dec 3. Epub 2021 Dec 3.

Wuxi School of Medicine, Jiangnan University, Wuxi, 214000, China.

Elevated blood pressure caused by excessive salt intake is common and associated with cardiovascular diseases in most countries. However, the composition and responses of vascular cells in the progression of hypertension have not been systematically described. We performed single-cell RNA sequencing on the aortic arch from C57BL/6J mice fed a chow/high-salt diet. Read More

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December 2021

Evaluating membrane structure by Laurdan imaging: Disruption of lipid packing by oxidized lipids.

Authors:
Irena Levitan

Curr Top Membr 2021 18;88:235-256. Epub 2021 Nov 18.

Division of Pulmonary and Critical Care, Department of Medicine, University of Illinois at Chicago, Chicago, IL, United States. Electronic address:

Impact of different lipids on membrane structure/lipid order is critical for multiple biological processes. Laurdan microscopy provides a unique tool to assess this property in heterogeneous biological membranes. This review describes the general principles of the approach and its application in model membranes and cells. Read More

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November 2021

Multifaceted Pathomolecular Mechanism of a VWF Large Deletion Involved in the Pathogenesis of Severe VWD.

Blood Adv 2021 Dec 3. Epub 2021 Dec 3.

Institute of Experimental Haematology and Transfusion Medicine, University Hospital Bonn, Germany.

An in-frame heterozygous large deletion of exons 4-34 of the von Willebrand factor (VWF) gene was identified in a type 3 von Willebrand disease (VWD) index patient (IP), as the only VWF variant. The IP exhibited severe bleeding episodes despite prophylaxis treatment, with a short VWF half-life after infusion of VWF/FVIII concentrates. Transcript analysis confirmed transcription of normal VWF mRNA besides an aberrant deleted transcript. Read More

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December 2021

Pinitol protects against ox-LDL-induced endothelial inflammation and monocytes attachment.

J Cardiovasc Pharmacol 2021 Dec 1. Epub 2021 Dec 1.

Department of Neurology, Nanchang First Hospital, Nanchang, Jiangxi, 330006, China.

Abstract: Atherosclerosis is a cardiovascular disease that affects a majority of people around the world at old age. Atherosclerosis is slow to develop and challenging to treat. Endothelial dysfunction caused by oxidative stress, inflammation, and other pathological factors drives the process of atherogenesis. Read More

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December 2021

Melatonin pre-treated bone marrow derived-mesenchymal stem cells prompt wound healing in rat models.

Biomed Pharmacother 2021 Nov 30;145:112473. Epub 2021 Nov 30.

Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt. Electronic address:

Bone marrow derived-mesenchymal stem cells (BMSCs)-based therapy is an outstanding candidate for cutaneous wound healing. Melatonin (MEL) has been reported for its anti-inflammatory as well as tissue regenerative properties. Existing work aimed to explore the potential healing power of BMSCs pre-treated with MEL in a skin wound model. Read More

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November 2021

Single cell transcriptomics reveal disrupted kidney filter cell-cell interactions after early and selective podocyte injury.

Am J Pathol 2021 Nov 30. Epub 2021 Nov 30.

The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA; Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA. Electronic address:

The health of the kidney filtration barrier requires communication between podocytes, endothelial cells and mesangial cells. Disruption of these cell-cell interactions is thought to contribute to disease progression in chronic kidney diseases (CKD). We recently demonstrated that podocyte ablation via doxycycline-inducible deletion of an essential endogenous molecule, CTCF (iCTCF), is sufficient to drive progressive CKD. Read More

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November 2021

Genetic deletion of Vegfr2 in endothelial cells leads to immediate disruption of tumor vessels and aggravation of hypoxia.

Am J Pathol 2021 Nov 30. Epub 2021 Nov 30.

Department of Anatomy. Electronic address:

Vascular endothelial growth factor (VEGF) blockers are used widely in clinics to target various types of human cancer. Although VEGF blockers exert marked tumor suppressive effects, the therapeutic effects can be limited. Moreover, accumulating evidence shows that VEGF acts not just on endothelial cells but also on various non-endothelial cells, including tumor and immune cells, suggesting a need to revisit the bona fide action of VEGF on endothelial cells using specific genetic mouse models. Read More

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November 2021

670nm photobiomodulation modulates bioenergetics and oxidative stress, in rat Müller cells challenged with high glucose.

PLoS One 2021 3;16(12):e0260968. Epub 2021 Dec 3.

Department of Biomedical Sciences, College of Health Sciences, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin, United States of America.

Diabetic retinopathy (DR), the most common complication of diabetes mellitus, is associated with oxidative stress, nuclear factor-κB (NFκB) activation, and excess production of vascular endothelial growth factor (VEGF) and intracellular adhesion molecule-1 (ICAM-1). Muller glial cells, spanning the entirety of the retina, are involved in DR inflammation. Mitigation of DR pathology currently occurs via invasive, frequently ineffective therapies which can cause adverse effects. Read More

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December 2021

Construction and Evaluation of a Bio-Engineered Pump to Enable Subpulmonary Support of the Fontan Circulation: A Proof-of-Concept Study.

ASAIO J 2021 Dec 1. Epub 2021 Dec 1.

From the Department of Pediatrics, Pediatric and Adult Congenital Cardiology, Baylor College of Medicine/Texas Children's Hospital, Houston, TX Biodesign, Texas Medical Center, Houston, TX Cullen Research Laboratory, Texas Heart Institute, Houston, TX Baylor College of Medicine, Houston, TX Morgan Advanced Materials Carbon Center of Excellence, Pennsylvania State University, State College, PA Department of Advanced CardioPulmonary Therapies and Transplantation, University of Texas Health Science Center, Houston, TX Department of Surgery, Congenital Heart Surgery, Baylor College of Medicine/Texas Children's Hospital, Houston, TX Regenerative Medicine Research, Texas Heart Institute, Houston, TX RegenMedix Consulting LLC.

Our objective was to create a bio-engineered pump (BEP) for subpulmonary Fontan circulation support capable of luminal endothelialization and producing a 2-6 mmHg pressure gradient across the device without flow obstruction. To accomplish this, porcine urinary bladder submucosa was decellularized to produce a urinary bladder matrix (UBM) which produced acellular sheets of UBM. The UBM was cultured with human umbilical vein endothelial cells producing a nearly confluent monolayer of cells with the maintenance of typical histologic features demonstrating UBM to be a suitable substrate for endothelial cells. Read More

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December 2021

Best Practices for Authentication of Cell Lines to Ensure Data Reproducibility and Integrity.

Radiat Res 2021 Dec 3. Epub 2021 Dec 3.

Division of Translational Radiation Sciences, Department of Radiation Oncology, University of Maryland School of Medicine, Baltimore, Maryland 21201.

Cell line misidentification and contamination are major contributors to the reproducibility crisis in academic research. Authentication of cell lines provides assurances of the data generated; however, commercially available cells are often not subjected to rigorous identification testing. In this study, commercially available cell lines underwent testing to confirm cell identity and purity. Read More

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December 2021

Rotigotine protects against oxidized low-density lipoprotein(ox-LDL)-induced damages in human umbilical vein endothelial cells(HUVECs).

Bioengineered 2021 Dec;12(2):10568-10579

Department of Cardiovascular Surgery, West China Hospital of Sichuan University, Chengdu, China.

Rotigotine is a non-ergoline dopamine agonist that has been licensed for the treatment of Parkinson's disease. Cardiovascular diseases are the world's leading cause of death. Ox-LDL- induced endothelial damages are involved in the initiation and progression of cardiovascular diseases. Read More

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December 2021

Chymase-positive Mast cells correlate with tumor angiogenesis: first report in pancreatic cancer patients.

Eur Rev Med Pharmacol Sci 2021 Nov;25(22):6862-6873

Interventional and Medical Oncology Unit, IRCCS Istituto Tumori "G. Paolo II", Bari, Italy.

Objective: Mast cells (MCs) are known to be involved in several physiological and pathological processes in humans and animals. Recently, their potential role in tumor development and angiogenesis has been investigated, arising interesting results to be potentially applied in clinics. Mast cells' granules contain a huge quantity of protease enzymes that, through different mechanisms, induce the formation of new microvessels, feeding tumor burden. Read More

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November 2021

Single-Cell RNA Sequencing of Mouse Left Ventricle Reveals Cellular Diversity and Intercommunication.

Physiol Genomics 2021 Dec 3. Epub 2021 Dec 3.

Shanghai University of Medicine and Health Sciences Affiliated Zhoupu Hospital and Shanghai Key Laboratory of Molecular Imaging, Shanghai, China.

Previous studies have revealed the diversity of the whole cardiac cellulome but not refined the left ventricle, which was essential for finding therapeutic targets. Here, we characterized single-cell transcriptional profiles of the mouse left ventricular cellular landscape using single-cell RNA sequencing (10×Genomics). Detailed t-Distributed Stochastic Neighbor Embedding (tSNE) analysis revealed the cell types of left ventricle with gene markers. Read More

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December 2021

Brain atrophy progression in Parkinson's disease is shaped by connectivity and local vulnerability.

Brain Commun 2021 17;3(4):fcab269. Epub 2021 Nov 17.

Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada.

Brain atrophy has been reported in the early stages of Parkinson's disease, but there have been few longitudinal studies. How intrinsic properties of the brain, such as anatomical connectivity, local cell-type distribution and gene expression combine to determine the pattern of disease progression also remains unknown. One hypothesis proposes that the disease stems from prion-like propagation of misfolded alpha-synuclein via the connectome that might cause varying degrees of tissue damage based on local properties. Read More

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November 2021

Role of Septins in Endothelial Cells and Platelets.

Front Cell Dev Biol 2021 11;9:768409. Epub 2021 Nov 11.

Department of Pediatrics and Adolescent Medicine, Division of Pediatric Hematology and Oncology, Medical Center, Faculty of Medicine, University of Freiburg, Freiburg, Germany.

Septins are conserved cytoskeletal GTP-binding proteins identified in almost all eukaryotes except higher plants. Mammalian septins comprise 13 family members with either ubiquitous or organ- and tissue-specific expression patterns. They form filamentous oligomers and complexes with other proteins to serve as diffusions barrier and/or multi-molecular scaffolds to function in a physiologically regulated manner. Read More

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November 2021

Identification of ACTA2 as a Key Contributor to Venous Malformation.

Front Cell Dev Biol 2021 8;9:755409. Epub 2021 Nov 8.

Department of Burn and Plastic Surgery, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

Proteomics and high connotation functional gene screening (HCS) were used to screen key functional genes that play important roles in the pathogenesis of venous malformation. Furthermore, this study was conducted to analyze and explore their possible functions, establish a gene mutation zebrafish model, and perform a preliminary study to explore their possible pathogenic mechanisms in venous malformation. Pathological and normal tissues from patients with disseminated venous malformation were selected for Tandem Mass Tag (TMT) proteomics analysis to identify proteins that were differentially expressed. Read More

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November 2021

Vascular Dysfunction in Malaria: Understanding the Role of the Endothelial Glycocalyx.

Front Cell Dev Biol 2021 10;9:751251. Epub 2021 Nov 10.

Duke University School of Medicine and Durham VA Medical Centers, Durham, NC, United States.

Malaria caused by results in over 400,000 deaths annually, predominantly affecting African children. In addition, non-falciparum species including vivax and knowlesi cause significant morbidity and mortality. Vascular dysfunction is a key feature in malaria pathogenesis leading to impaired blood perfusion, vascular obstruction, and tissue hypoxia. Read More

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November 2021

Establishment of Functional Liver Spheroids From Human Hepatocyte-Derived Liver Progenitor-Like Cells for Cell Therapy.

Front Bioeng Biotechnol 2021 8;9:738081. Epub 2021 Nov 8.

Department of Anesthesiology and Critical Care Medicine, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Globally, about two million people die from liver diseases every year. Liver transplantation is the only reliable therapy for severe end-stage liver disease, however, the shortage of organ donors is a huge limitation. Human hepatocytes derived liver progenitor-like cells (HepLPCs) have been reported as a novel source of liver cells for development of models, cell therapies, and tissue-engineering applications, but their functionality as transplantation donors is unclear. Read More

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November 2021

A Small Ligand That Selectively Binds to the G-quadruplex at the Human Vascular Endothelial Growth Factor Internal Ribosomal Entry Site and Represses the Translation.

Front Chem 2021 9;9:781198. Epub 2021 Nov 9.

State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang, China.

G-quadruplexes are believed to have important biological functions, so many small molecules have been screened or developed for targeting G-quadruplexes. However, it is still a major challenge to find molecules that recognize specific G-quadruplexes. Here, by using a combination of surface plasmon resonance, electrospray ionization mass spectrometry, circular dichroism, Western blot, luciferase assay, and reverse transcriptase stop assay, we observed a small molecule, namely, oxymatrine (OMT) that could selectively bind to the RNA G-quadruplex in 5'-untranslated regions (UTRs) of human vascular endothelial growth factor (hVEGF), but could not bind to other G-quadruplexes. Read More

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November 2021

Use of Eculizumab in Pediatric Patients With Transplant Associated Thrombotic Microangiopathy.

Front Pediatr 2021 11;9:761726. Epub 2021 Nov 11.

Pediatric Oncology and Hematology Service, Hematopoietic Stem Cell Transplantation Section, Vall d'Hebron University Hospital, Barcelona, Spain.

Transplant-associated thrombotic microangiopathy (TA-TMA) is a serious complication of hematopoietic stem cell transplantation (HSCT) associated with high morbidity and mortality. High-risk TA-TMA (hrTA-TMA) is characterized by multifactorial endothelial damage caused by environmental stressors, dysregulation of the complement system, and genetic predisposition. Complement inhibitors have significantly decreased mortality and are the current treatment of choice. Read More

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November 2021

The Effect of STAT3 Signal Pathway Activation on Retinopathy of Prematurity.

Front Pediatr 2021 10;9:638432. Epub 2021 Nov 10.

Department of Neonatology, Guangdong Women and Children Hospital, Guangzhou, China.

To investigate the mechanism of activation of the signal transducer and activator of transcription 3 (STAT3) signal pathway in the process of retinopathy of prematurity (ROP). Sixty newborn Sprague-Dawley (SD) rats were randomly separated into the hyperoxia and air control groups ( = 30/in each group). The serum hepcidin level on 21 d was measured using the enzyme-linked immunosorbent assay (ELISA). Read More

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November 2021

EMS1/DLL4-Notch Signaling Axis Augments Cell Cycle-Mediated Tumorigenesis and Progress in Human Adrenocortical Carcinoma.

Front Oncol 2021 10;11:771579. Epub 2021 Nov 10.

Department of Immunology, Nankai University School of Medicine, Tianjin, China.

Adrenocortical carcinoma (ACC) is a rare malignant neoplasm that is prone to local invasion and metastasis. Meanwhile, overexpressed endothelial cell-specific molecule 1 () is closely related to tumorigenesis of multitudinous tumors. However, the prognosis value and biological function of in ACC remains undefined. Read More

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November 2021

Vascularized bone regeneration accelerated by 3D-printed nanosilicate-functionalized polycaprolactone scaffold.

Regen Biomater 2021 Dec 12;8(6):rbab061. Epub 2021 Nov 12.

Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key Laboratory of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350002, China.

Critical oral-maxillofacial bone defects, damaged by trauma and tumors, not only affect the physiological functions and mental health of patients but are also highly challenging to reconstruct. Personalized biomaterials customized by 3D printing technology have the potential to match oral-maxillofacial bone repair and regeneration requirements. Laponite (LAP) nanosilicates have been added to biomaterials to achieve biofunctional modification owing to their excellent biocompatibility and bioactivity. Read More

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December 2021

Effects of SDF-1/CXCR7 on the Migration, Invasion and Epithelial-Mesenchymal Transition of Gastric Cancer Cells.

Front Genet 2021 9;12:760048. Epub 2021 Nov 9.

Department of Gastroenterology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

We found that SDF-1/CXCR7 axis played an important role in the growth and proliferation of gastric cancer in the previous studies. The objectives of this study were to explore the effects of SDF-1/CXCR7 on the metastatic ability of gastric cancer cells and the possible mechanisms. CXCR7 expression in SGC-7901 gastric cancer cells was stably knocked down via lentiviral vectors. Read More

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November 2021

Expanded Hemodialysis Therapy Ameliorates Uremia-Induced Systemic Microinflammation and Endothelial Dysfunction by Modulating VEGF, TNF-α and AP-1 Signaling.

Front Immunol 2021 11;12:774052. Epub 2021 Nov 11.

Department of Nephrology and Internal Intensive Care Medicine, Charité Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health (BIH), Berlin, Germany.

Abstract: Systemic chronic microinflammation and altered cytokine signaling, with adjunct cardiovascular disease (CVD), endothelial maladaptation and dysfunction is common in dialysis patients suffering from end-stage renal disease and associated with increased morbidity and mortality. New hemodialysis filters might offer improvements. We here studied the impact of novel improved molecular cut-off hemodialysis filters on systemic microinflammation, uremia and endothelial dysfunction. Read More

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November 2021