ASAIO J 2021 Dec 1. Epub 2021 Dec 1.
From the Department of Pediatrics, Pediatric and Adult Congenital Cardiology, Baylor College of Medicine/Texas Children's Hospital, Houston, TX Biodesign, Texas Medical Center, Houston, TX Cullen Research Laboratory, Texas Heart Institute, Houston, TX Baylor College of Medicine, Houston, TX Morgan Advanced Materials Carbon Center of Excellence, Pennsylvania State University, State College, PA Department of Advanced CardioPulmonary Therapies and Transplantation, University of Texas Health Science Center, Houston, TX Department of Surgery, Congenital Heart Surgery, Baylor College of Medicine/Texas Children's Hospital, Houston, TX Regenerative Medicine Research, Texas Heart Institute, Houston, TX RegenMedix Consulting LLC.
Our objective was to create a bio-engineered pump (BEP) for subpulmonary Fontan circulation support capable of luminal endothelialization and producing a 2-6 mmHg pressure gradient across the device without flow obstruction. To accomplish this, porcine urinary bladder submucosa was decellularized to produce a urinary bladder matrix (UBM) which produced acellular sheets of UBM. The UBM was cultured with human umbilical vein endothelial cells producing a nearly confluent monolayer of cells with the maintenance of typical histologic features demonstrating UBM to be a suitable substrate for endothelial cells. Read More