Vet Microbiol 2021 Apr 10;257:109066. Epub 2021 Apr 10.
Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD, United States; Department of Population Medicine and Diagnostic Sciences, Animal Health Diagnostic Center, College of Veterinary Medicine, Cornell University, 240 Farrier Rd, Ithaca, NY, 14853, United States; Laboratório de Patologia Veterinária, Departamento de Patologia, Universidade Federal de Santa Maria, Av. Roraima, 1000, Santa Maria, Rio Grande do Sul, 97105-900, Brazil. Electronic address:
The parapoxvirus orf virus (ORFV) encodes several immunomodulatory proteins (IMPs) that modulate host innate and pro-inflammatory responses to infection. Using the ORFV IA82 strain as the parental virus, recombinant viruses with individual deletions in the genes encoding the IMPs chemokine binding protein (CBP; ORFV112), inhibitor of granulocyte-monocyte colony-stimulating factor and IL-2 (GIF, ORFV117) and interleukin 10 homologue (vIL-10; ORFV127) were generated and characterized in vitro and in vivo. The replication properties of the individual gene deletion viruses in cell culture was not affected comparing with the parental virus. Read More