150 results match your criteria enables snapshot

A single snapshot multiplex immunoassay platform utilizing dense test lines based on engineered beads.

Biosens Bioelectron 2021 May 31;190:113388. Epub 2021 May 31.

Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Cheongam-ro77, Nam-gu, Pohang-si, Gyeongsangbuk-do, 37673, South Korea. Electronic address:

Co-circulation of coronavirus disease 2019 (COVID-19) and dengue fever has been reported. Accurate and timely multiplex diagnosis is required to prevent future pandemics. Here, we developed an innovative microfluidic chip that enables a snapshot multiplex immunoassay for timely on-site response and offers unprecedented multiplexing capability with an operating procedure similar to that of lateral flow assays. Read More

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Single-shot detection of 8 unique monochrome fringe patterns representing 4 distinct directions via multispectral fringe projection profilometry.

Sci Rep 2021 May 14;11(1):10367. Epub 2021 May 14.

Imaging Program, Lawson Health Research Institute, St. Joseph's Health Care, London, ON, N6A 4V2, Canada.

Spatial resolution in three-dimensional fringe projection profilometry is determined in large part by the number and spacing of fringes projected onto an object. Due to the intensity-based nature of fringe projection profilometry, fringe patterns must be generated in succession, which is time-consuming. As a result, the surface features of highly dynamic objects are difficult to measure. Read More

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Ultrafast light field tomography for snapshot transient and non-line-of-sight imaging.

Nat Commun 2021 04 12;12(1):2179. Epub 2021 Apr 12.

Department of Bioengineering, University of California, Los Angeles, CA, USA.

Cameras with extreme speeds are enabling technologies in both fundamental and applied sciences. However, existing ultrafast cameras are incapable of coping with extended three-dimensional scenes and fall short for non-line-of-sight imaging, which requires a long sequence of time-resolved two-dimensional data. Current non-line-of-sight imagers, therefore, need to perform extensive scanning in the spatial and/or temporal dimension, restricting their use in imaging only static or slowly moving objects. Read More

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Poly-A Tailing and Adaptor Ligation Methods for Ribo-Seq Library Construction.

Methods Mol Biol 2021 ;2252:221-237

Division of Nutritional Sciences, Cornell University, Ithaca, NY, USA.

Ribosome profiling is a powerful technique that enables researchers to monitor translational events across the transcriptome. It provides a snapshot of ribosome positions and density across the transcriptome at a sub-codon resolution. Here we describe the whole procedure of profiling ribosome footprints in mammalian cells. Read More

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Multi-aperture system approach for snapshot multispectral imaging applications.

Opt Express 2021 Mar;29(5):7361-7378

We present an ultra-compact system approach for snapshot, multispectral imaging. It is based on a slanted linear variable spectral filter mounted in close proximity to the entrance pupil of a micro-optical, multi-aperture imaging system. A compact demonstration setup with a size of only 60 × 60 × 28 mm is developed, which enables the acquisition of 66 spectral channels in a single shot and offers a linear spectral sampling of approximately six nanometers over an extended wavelength range of 450-850 nm. Read More

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MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria.

J Vis Exp 2021 02 23(168). Epub 2021 Feb 23.

Université de Strasbourg, CNRS;

Although small regulatory RNAs (sRNAs) are widespread among the bacterial domain of life, the functions of many of them remain poorly characterized notably due to the difficulty of identifying their mRNA targets. Here, we described a modified protocol of the MS2-Affinity Purification coupled with RNA Sequencing (MAPS) technology, aiming to reveal all RNA partners of a specific sRNA in vivo. Broadly, the MS2 aptamer is fused to the 5' extremity of the sRNA of interest. Read More

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February 2021

Enhancement of CASSI by a zero-order image employing a single detector.

Appl Opt 2021 Feb;60(5):1463-1469

Coded aperture snapshot spectral imaging (CASSI) makes it possible to recover 3D hyperspectral data from a single 2D image. However, the reconstruction problem is severely underdetermined, and efforts to improve the compression ratio typically make the imaging system more complex and cause a significant loss of incoming light intensity. In this paper, we propose a novel approach to CASSI that enables capturing both a spectrally sheared and an integrated image of a scene with a single camera. Read More

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February 2021

Noninvasive quantification of target availability during therapy using paired-agent fluorescence tomography.

Theranostics 2020 14;10(24):11230-11243. Epub 2020 Sep 14.

Thayer School of Engineering, Dartmouth College, Hanover, NH 03755.

Immuno-oncological treatment strategies that target abnormal receptor profiles of tumors are an increasingly important feature of cancer therapy. Yet, assessing receptor availability (RA) and drug-target engagement, important determinants of therapeutic efficacy, is challenging with current imaging strategies, largely due to the complex nonspecific uptake behavior of imaging agents in tumors. Herein, we evaluate whether a quantitative noninvasive imaging approach designed to compensate for nonspecific uptake, MRI-coupled paired-agent fluorescence tomography (MRI-PAFT), is capable of rapidly assessing the availability of epidermal growth factor receptor (EGFR) in response to one dose of anti-EGFR antibody therapy in orthotopic brain tumor models. Read More

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Combustion in the future: The importance of chemistry.

Proc Combust Inst 2020 Sep 25. Epub 2020 Sep 25.

Department of Chemistry, Bielefeld University, Universitätsstraße 25, D-33615 Bielefeld, Germany.

Combustion involves chemical reactions that are often highly exothermic. Combustion systems utilize the energy of chemical compounds released during this reactive process for transportation, to generate electric power, or to provide heat for various applications. Chemistry and combustion are interlinked in several ways. Read More

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September 2020

Design of binary-phase diffusers for a compressed sensing snapshot spectral imaging system with two cameras.

Appl Opt 2020 Sep;59(26):7853-7864

We propose designs of pupil-domain optical diffusers for a snapshot spectral imaging system using binary-phase encoding. The suggested designs enable the creation of point-spread functions with defined optical response, having profiles that are dependent on incident wavefront wavelength. This efficient combination of dispersive and diffusive optical responses enables us to perform snapshot spectral imaging using compressed sensing algorithms while keeping a high optical throughput alongside a simple fabrication process. Read More

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September 2020

Snapshot spectral imaging with parallel metasystems.

Sci Adv 2020 Sep 18;6(38). Epub 2020 Sep 18.

Department of Electrical and Computer Engineering, University of Massachusetts Amherst, 151 Holdsworth Way, Amherst, MA 01003, USA.

Spectral imagers divide scenes into quantitative and narrowband spectral channels. They have become important metrological tools in many areas of science, especially remote sensing. Here, we propose and experimentally demonstrate a snapshot spectral imager using a parallel optical processing paradigm based on arrays of metasystems. Read More

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September 2020

Pharmaceutical Advances and Proteomics Researches.

Iran J Pharm Res 2019 ;18(Suppl1):51-67

Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Proteomics enables understanding the composition, structure, function and interactions of the entire protein complement of a cell, a tissue, or an organism under exactly defined conditions. Some factors such as stress or drug effects will change the protein pattern and cause the present or absence of a protein or gradual variation in abundances. The aim of this study is to explore relationship between proteomics application and drug discovery. Read More

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January 2019

Proximity-CLIP Provides a Snapshot of Protein-Occupied RNA Elements at Subcellular Resolution and Transcriptome-Wide Scale.

Methods Mol Biol 2020 ;2166:283-305

Laboratory of Muscle Stem Cells and Gene Regulation, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, USA.

The distribution of messenger RNAs (mRNAs) to specific subcellular locations has been studied for the past two decades. Technically, studies of RNA localization are lagging those related to protein localization. Here we provide a detailed protocol for Proximity-CLIP, a method recently developed by our group, that combines proximity biotinylation of proteins with photoactivatable ribonucleoside-enhanced protein-RNA cross-linking to simultaneously profile the proteome including RNA-binding proteins (RBPs) and the RBP-bound transcriptome in any given subcellular compartment. Read More

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A combination of genome-wide association study screening and SNaPshot for detecting sex-related SNPs and genes in Cynoglossus semilaevis.

Comp Biochem Physiol Part D Genomics Proteomics 2020 09 9;35:100711. Epub 2020 Jul 9.

Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China. Electronic address:

Chinese tongue sole (Cynoglossus semilaevis) males and females exhibit great differences in growth rate and appearance. The species is heterogametic (ZW/ZZ) and has sex-reversed "pseudomales" that are genetically female and physiologically male. In this study, we identified eight sex-specific single nucleotide polymorphism (SNP) markers for the sex identification of C. Read More

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September 2020

Whole brain snapshot CEST at 3T using 3D-EPI: Aiming for speed, volume, and homogeneity.

Magn Reson Med 2020 11 9;84(5):2469-2483. Epub 2020 May 9.

High-field Magnetic Resonance Center, Max Planck Institute for Biological Cybernetics, Tuebingen, Germany.

Purpose: CEST MRI enables imaging of distributions of low-concentrated metabolites as well as proteins and peptides and their alterations in diseases. CEST examinations often suffer from low spatial resolution, long acquisition times, and concomitant motion artifacts. This work aims to maximize both resolution and volume coverage with a 3D-EPI snapshot CEST approach at 3T, allowing for fast and robust whole-brain CEST MRI. Read More

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November 2020

Transcriptome analysis of drought-tolerant sorghum genotype SC56 in response to water stress reveals an oxidative stress defense strategy.

Mol Biol Rep 2020 May 17;47(5):3291-3303. Epub 2020 Apr 17.

USDA-ARS Forage-Animal Production Research Unit, 1100 Limestone Rd, Lexington, KY, 40546, USA.

Drought tolerance is a crucial trait for crops to curtail the yield loss inflicted by water stress, yet genetic improvement efforts are challenged by the complexity of this character. The adaptation of sorghum to abiotic stress, its genotypic variability, and relatively small genome make this species well-suited to dissect the molecular basis of drought tolerance. The use of differential transcriptome analysis provides a snapshot of the bioprocesses underlying drought response as well as genes that might be determinants of the drought tolerance trait. Read More

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Analysis of the nucleocytoplasmic shuttling RNA-binding protein HNRNPU using optimized HITS-CLIP method.

PLoS One 2020 17;15(4):e0231450. Epub 2020 Apr 17.

Department of Physiology, School of Medicine, Keio University, Minato, Japan.

RNA-binding proteins (RBPs) control many types of post-transcriptional regulation, including mRNA splicing, mRNA stability, and translational efficiency, by directly binding to their target RNAs and their mutation and dysfunction are often associated with several human neurological diseases and tumorigenesis. Crosslinking immunoprecipitation (CLIP), coupled with high-throughput sequencing (HITS-CLIP), is a powerful technique for investigating the molecular mechanisms underlying disease pathogenesis by comprehensive identification of RBP target sequences at the transcriptome level. However, HITS-CLIP protocol is still required for some optimization due to experimental complication, low efficiency and time-consuming, whose library has to be generated from very small amounts of RNAs. Read More

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Cargo hold and delivery: Ankyrins, spectrins, and their functional patterning of neurons.

Cytoskeleton (Hoboken) 2020 03 14;77(3-4):129-148. Epub 2020 Feb 14.

Department of Cell Biology and Physiology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.

The highly polarized, typically very long, and nonmitotic nature of neurons present them with unique challenges in the maintenance of their homeostasis. This architectural complexity serves a rich and tightly controlled set of functions that enables their fast communication with neighboring cells and endows them with exquisite plasticity. The submembrane neuronal cytoskeleton occupies a pivotal position in orchestrating the structural patterning that determines local and long-range subcellular specialization, membrane dynamics, and a wide range of signaling events. Read More

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Validation of a 52-mtSNP minisequencing panel for haplogroup classification of forensic DNA samples.

Int J Legal Med 2020 May 6;134(3):929-936. Epub 2020 Feb 6.

BIOMICs Research Group, Lascaray Research Center, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain.

Mitochondrial DNA (mtDNA) is a useful tool in forensic investigation as it provides information about the matrilineal ancestry of individuals. In addition, mtDNA can be analyzed when the analysis of other nuclear markers is underperforming. Recently, we developed a minisequencing panel for the simultaneous analysis of 52 mtDNA SNPs to classify maternal lineages into the main haplogroups and their phylogeographic origin. Read More

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An all-optical technique enables instantaneous single-shot demodulation of images at high frequency.

Nat Commun 2020 Jan 28;11(1):549. Epub 2020 Jan 28.

Univ Rennes, CNRS, Institut FOTON-UMR 6082, F-35000, Rennes, France.

High-frequency demodulation of wide area optical signals in a snapshot manner remains a technological challenge. If solved, it could open tremendous perspectives in 3D imaging, vibrometry, free-space communications, automated vision, or ballistic photon imaging in scattering media with numerous applications in smart autonomous vehicles and medical diagnosis. We present here a snapshot quadrature demodulation imaging technique, capable of estimating the amplitude and phase from a single acquisition, without synchronization of emitter and receiver, and with the added capability of continuous frequency tuning. Read More

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January 2020

Grayscale-to-Color: Scalable Fabrication of Custom Multispectral Filter Arrays.

ACS Photonics 2019 Dec 23;6(12):3132-3141. Epub 2019 Oct 23.

Department of Physics, Cavendish Laboratory, University of Cambridge, JJ Thomson Avenue, Cambridge, CB3 0HE, U.K.

Snapshot multispectral image (MSI) sensors have been proposed as a key enabler for a plethora of multispectral imaging applications, from diagnostic medical imaging to remote sensing. With each application requiring a different set, and number, of spectral bands, the absence of a scalable, cost-effective manufacturing solution for custom multispectral filter arrays (MSFAs) has prevented widespread MSI adoption. Despite recent nanophotonic-based efforts, such as plasmonic or high-index metasurface arrays, large-area MSFA manufacturing still consists of many-layer dielectric (Fabry-Perot) stacks, requiring separate complex lithography steps for each spectral band and multiple material compositions for each. Read More

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December 2019

The emergence and promise of single-cell temporal-omics approaches.

Curr Opin Biotechnol 2020 06 6;63:70-78. Epub 2020 Jan 6.

Laboratory of Neurodevelopmental Systems Biology, Brain Mind Institute, School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland. Electronic address:

Single-cell transcriptomics enables the measurement of gene expression in complex biological systems at the resolution of individual cells. Multivariate analysis of single-cell data helps describe the variation in expression accompanying cellular processes during embryonic development, disease progression and in response to stimuli. Likewise, new methods have extended the possibilities of single-cell analysis by measuring the transcriptome while simultaneously capturing information on lineage or past molecular events. Read More

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Mass Spectrometry: A Guide for the Clinician.

J Clin Exp Hepatol 2019 Sep-Oct;9(5):597-606. Epub 2019 May 9.

Division of Surgery and Cancer, Imperial College London, London, W2 INY, United Kingdom.

Metabolic profiling, metabonomics and metabolomics are terms coined in the late 1990s as they emerged as the newest '' technology at the time. This line of research enquiry uses spectroscopic analytical platforms, which are mainly nuclear magnetic resonance spectroscopy and mass spectrometry (MS), to acquire a snapshot of metabolites, the end products of a complex biological system. Metabolic profiling enables the detection, quantification and characterisation of metabolites in biofluids, cells and tissues. Read More

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Spectral recording of gene expression history by fluorescent timer protein.

Biotechniques 2019 10 6;67(4):154-164. Epub 2019 Sep 6.

Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), Campus Vienna Biocenter 1, 1030 Vienna, Austria.

Monitoring spatio-temporal patterns of gene expression by fluorescent proteins requires longitudinal observation, which is often difficult to implement. Here, we fuse a fluorescent timer (FT) protein with an immediate early gene (IEG) promoter to track live gene expression in single cells. This results in a stimulus- and time-dependent spectral shift from blue to red for subsequent monitoring with fluorescence activated cell sorting (FACS) and live cell imaging. Read More

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October 2019

Assistive technology enables inclusion in higher education: The role of Higher and Further Education Disability Services Association.

Afr J Disabil 2019 22;8:558. Epub 2019 Aug 22.

Disability Unit, Centre for Student Counselling and Development, Stellenbosch University, Stellenbosch, South Africa.

Background: Using assistive technology is one way to foster inclusion of students in the post-school education and training (PSET) sector.

Objectives: Higher and Further Education Disability Services Association (HEDSA) enables the sharing of new knowledge about assistive technologies through its symposia, and making information available on its website. Additionally, it facilitates dialogue and collaboration amongst institutions in the PSET network using a listserv and newsletters, given that PSET institutions are spread countrywide. Read More

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The Past and Future of Experimental Speciation.

Trends Ecol Evol 2020 01 12;35(1):10-21. Epub 2019 Sep 12.

Department of Animal and Plant Sciences, University of Sheffield, Sheffield, S10 2TN, UK. Electronic address:

Speciation is the result of evolutionary processes that generate barriers to gene flow between populations, facilitating reproductive isolation. Speciation is typically studied via theoretical models and snapshot tests in natural populations. Experimental speciation enables real-time direct tests of speciation theory and has been long touted as a critical complement to other approaches. Read More

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January 2020

Optimized design, calibration, and validation of an achromatic snapshot full-Stokes imaging polarimeter.

Opt Express 2019 Aug;27(16):23009-23028

An achromatic snapshot full-Stokes imaging polarimeter (ASSIP) that enables the acquisition of 2D-spatial full Stokes parameters from a single exposure is presented. It is based on the division-of-aperture polarimetry using an array of four-quadrant achromatic elliptical analyzers as polarization state analyzer (PSA). The optimization of PSA is addressed for achieving immunity of Gaussian and Poisson noises. Read More

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Combined X-ray diffraction and absorption tomography using a conical shell beam.

Opt Express 2019 Jul;27(15):21092-21101

We combine diffraction and absorption tomography by raster scanning samples through a hollow cone of pseudo monochromatic X-rays with a mean energy of 58.4 keV. A single image intensifier takes 90x90 (x,y) snapshots during the scan. Read More

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Confocal energy-dispersive X-ray diffraction tomography employing a conical shell beam.

Opt Express 2019 Jul;27(14):19834-19841

We introduce a new high-energy X-ray diffraction tomography technique for volumetric materials characterization. In this method, a conical shell beam is raster scanned through the samples. A central aperture optically couples the diffracted flux from the samples onto a pixelated energy-resolving detector. Read More

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Revealing angular momentum transfer channels and timescales in the ultrafast demagnetization process of ferromagnetic semiconductors.

Proc Natl Acad Sci U S A 2019 Sep 9;116(39):19258-19263. Epub 2019 Sep 9.

Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720;

Ultrafast control of magnetic order by light provides a promising realization for spintronic devices beyond Moore's Law and has stimulated intense research interest in recent years. Yet, despite 2 decades of debates, the key question of how the spin angular momentum flows on the femtosecond timescale remains open. The lack of direct first-principle methods and pictures for such process exacerbates the issue. Read More

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September 2019