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Circulating tumor cells with epithelial-mesenchymal transition markers as potential biomarkers for the diagnosis of lung cancer.

World J Clin Cases 2021 Apr;9(12):2721-2730

Department of Thoracic Surgery, Institute of Surgery Research, Daping Hospital, Army Medical University, Chongqing 400042, China.

Background: Circulating tumor cells (CTCs) can be clustered into three subtypes according to epithelial-mesenchymal transition (EMT) markers: CTCs with epithelial markers (E-CTCs), CTCs with mesenchymal markers (M-CTCs), and CTCs with both markers (E&M-CTCs). CTC detection has clinical implications in the diagnosis of lung cancer (LC).

Aim: To clarify the diagnostic value of CTCs categorized by EMT markers in LC. Read More

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ADORA1 promotes nasopharyngeal carcinoma cell progression through regulation of PI3K/AKT/GSK-3β/β-catenin signaling.

Life Sci 2021 May 4;278:119581. Epub 2021 May 4.

Department of Radiation Oncology, Yue Bei People's Hospital, Shaoguan, Guangdong, China.

Aims: For most human cancers, the expression pattern and biological function of ADORA1 (Adenosine A1 Receptor) are largely unknown. This study has been designed to explore the clinical significance and the mechanism of ADORA1 in nasopharyngeal carcinoma (NPC) cells.

Materials And Methods: The level of ADORA1 in NPC and its adjacent tissues was analyzed by IHC, real-time PCR and western blotting. Read More

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The comprehensive landscape of miR-34a in cancer research.

Cancer Metastasis Rev 2021 May 6. Epub 2021 May 6.

New Drug Screening Center, China Pharmaceutical University, Nanjing, 210009, China.

MicroRNA-34 (miR-34) plays central roles in human diseases, especially cancers. Inactivation of miR-34 is detected in cancer cell lines and tumor tissues versus normal controls, implying its potential tumor-suppressive effect. Clinically, miR-34 has been identified as promising prognostic indicators for various cancers. Read More

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Snail-Family Proteins: Role in Carcinogenesis and Prospects for Antitumor Therapy.

Acta Naturae 2021 Jan-Mar;13(1):76-90

Blokhin National Medical Research Center of Oncology, Moscow, 115478 Russia.

The review analyzes Snail family proteins, which are transcription factors involved in the regulation of the epithelial-mesenchymal transition (EMT) of tumor cells. We describe the structure of these proteins, their post-translational modification, and the mechanisms of Snail-dependent regulation of genes. The role of Snail proteins in carcinogenesis, invasion, and metastasis is analyzed. Read More

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Knockdown of circ_0000144 suppresses cell proliferation, migration and invasion in gastric cancer through via sponging miR-217.

J Microbiol Biotechnol 2021 May 5;31(1). Epub 2021 May 5.

Department of General Surgery, Sunshine Union Hospital, High-tech Dstrict, Weifang, 261000, China.

Previous studies have uncovered the role of circ_0000144 in various tumors. The present study investigated the function and mechanism of circ_0000144 in the gastric cancer (GC) progression.The expression of circ_0000144 in GC tissues and cells were detected through quantitative real-time polymerase chain reaction (qRT-PCR) method. Read More

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SATB1 protein is associated with the epithelial‑mesenchymal transition process in non‑small cell lung cancers.

Oncol Rep 2021 Jun 6;45(6). Epub 2021 May 6.

Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50‑368 Wroclaw, Poland.

Lung cancer is one of the most frequently diagnosed neoplasms and the leading cause of cancer‑related mortality worldwide. Its predominant subtype is non‑small cell lung cancer (NSCLC), which accounts for over 80% of the cases. Surprisingly, the majority of lung cancer‑related deaths are caused not by a primary tumour itself, but by its metastasis to distant organs. Read More

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MiR-140-3p Impedes Gastric Cancer Progression and Metastasis by Regulating BCL2/BECN1-Mediated Autophagy.

Onco Targets Ther 2021 29;14:2879-2892. Epub 2021 Apr 29.

Department of General Surgery, People's Hospital of Jingjiang, Taizhou, 214500, Jiangsu, People's Republic of China.

Introduction: MiRNAs have been proven to modulate the progression of gastric cancer (GC). In this field, we evaluated the role and mechanism of miR-140-3p in GC.

Methods: Western blotting and qRT-PCR were used to detect the levels of miR-140-3p and BCL2. Read More

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SOX2 promotes chemoresistance, cancer stem cells properties, and epithelial-mesenchymal transition by β-catenin and Beclin1/autophagy signaling in colorectal cancer.

Cell Death Dis 2021 May 5;12(5):449. Epub 2021 May 5.

Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Sex-determining region Y-box2 (SOX2), a master regulator of embryonic and induced pluripotent stem cells, drives cancer stem cells (CSCs) properties, fuels tumor initiation, and contributes to tumor aggressiveness. Our previous study has demonstrated the oncogenic role of SOX2 in colorectal cancer (CRC). In this study, we sought to elucidate the underlying mechanisms. Read More

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ABCB1 inhibition provides a novel therapeutic target to block TWIST1-induced migration in medulloblastoma.

Neurooncol Adv 2021 Jan-Dec;3(1):vdab030. Epub 2021 Apr 28.

Children's Brain Tumour Research Centre, Division of Child Health, Obstetrics and Gynaecology, School of Medicine, University of Nottingham, Nottingham, UK.

Background: Therapeutic intervention in metastatic medulloblastoma is dependent on elucidating the underlying metastatic mechanism. We investigated whether an epithelial-mesenchymal transition (EMT)-like pathway could drive medulloblastoma metastasis.

Methods: A 3D Basement Membrane Extract (3D-BME) model was used to investigate medulloblastoma cell migration. Read More

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Involvement of BMP and Wnt Signals Leadingto Epithelial-Mesenchymal Transition in Colon Adenocarcinoma with Heterotopic Ossification.

Ann Clin Lab Sci 2021 Mar;51(2):271-276

Department of Cancer Pathology, Faculty of Medicine, Hokkaido University, Sapporo

Here we present the case of a 73-year-old male with rectal adenocarcinoma with heterotopic ossification (HO). Cancer-associated HO in the digestive system is rare. Thus, the precise mechanism and clinicopathological significance of HO have not yet been defined. Read More

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Taurine Attenuates the Hypotaurine-Induced Progression of CRC ERK/RSK Signaling.

Front Cell Dev Biol 2021 15;9:631163. Epub 2021 Apr 15.

Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.

Colorectal cancer (CRC) is one of the most common malignant tumors, and previous metabolomics work has demonstrated great promise in identifying specific small molecules of tumor phenotype. In the present study, we analyzed the metabolites of resected tissues through gas chromatography-mass spectrometry (GC-MS), and found that the concentration of taurine in CRC tissues diminished whereas the concentration of hypotaurine increased. The results demonstrated that taurine significantly suppressed cellular proliferation, metastasis, and colony formation whereas it induced apoptosis in CRC cells. Read More

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miR-139-5p Loss-Mediated WTAP Activation Contributes to Hepatocellular Carcinoma Progression by Promoting the Epithelial to Mesenchymal Transition.

Front Oncol 2021 15;11:611544. Epub 2021 Apr 15.

Clinical Laboratory Diagnostics, Medical Technology College, Beihua University, Jilin, China.

Hepatocellular carcinoma (HCC) is a primary aggressive gastrointestinal neoplasm that affects patients worldwide. It has been shown that Wilms' tumor 1-associating protein (WTAP) is frequently upregulated in various cancers. However, the potential role of WTAP in HCC remains largely unknown. Read More

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The lnc-CTSLP8 upregulates CTSL1 as a competitive endogenous RNA and promotes ovarian cancer metastasis.

J Exp Clin Cancer Res 2021 May 1;40(1):151. Epub 2021 May 1.

Department of Gynecology and Obstetrics, XinHua Hospital, Shanghai JiaoTong University School of Medicine, 1665 Kongjiang Rd, Yangpu District, Shanghai, 200092, China.

Background: Ovarian cancer is highly lethal and has a poor prognosis due to metastasis. Long non-coding RNAs (lncRNAs) are key regulators of tumor development, but their role in ovarian cancer metastasis remains unclear.

Methods: The expression of lnc-CTSLP8 in ovarian cancer was analyzed in public databases (TCGA and GEO) and validated via qRT-PCR. Read More

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TNF-α/NF-κB signaling epigenetically represses PSD4 transcription to promote alcohol-related hepatocellular carcinoma progression.

Cancer Med 2021 May 1. Epub 2021 May 1.

Department of Infectious Disease, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.

Background: Chronic alcohol consumption is more frequently associated with advanced, aggressive hepatocellular carcinoma (HCC) tumors. Alcohol adversely impacts ER/Golgi membrane trafficking and Golgi protein N-glycosylation in hepatocytes; these effects have been attributed (in part) to dysregulated adenosine diphosphate-ribosylation factor (ARF) GTPase signaling. Here, we investigated the role of the ARF GTPase guanine exchange factor PSD4 in HCC progression. Read More

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Optimization of β-1,4-Endoxylanase Production by an Strain Growing on Wheat Straw and Application in Xylooligosaccharides Production.

Molecules 2021 Apr 26;26(9). Epub 2021 Apr 26.

Biotechnology for Lignocellulosic Biomass Group, Centro de Investigaciones Biológicas (CIB-CSIC), C/Ramiro de Maeztu 9, 28040 Madrid, Spain.

Plant biomass constitutes the main source of renewable carbon on the planet. Its valorization has traditionally been focused on the use of cellulose, although hemicellulose is the second most abundant group of polysaccharides on Earth. The main enzymes involved in plant biomass degradation are glycosyl hydrolases, and filamentous fungi are good producers of these enzymes. Read More

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Ubiquitin Conjugating Enzyme E2 H (UBE2H) Is Linked to Poor Outcomes and Metastasis in Lung Adenocarcinoma.

Biology (Basel) 2021 Apr 28;10(5). Epub 2021 Apr 28.

Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.

The prognosis of patients with metastatic lung adenocarcinoma (LUAD) is poor. Although novel lung cancer treatments have been developed for metastatic LUAD, not all patients are fit to receive these treatments. The present study aimed to identify the novel regulatory genes in metastatic LUAD. Read More

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A Reversible Shift of Driver Dependence from EGFR to Notch1 in Non-Small Cell Lung Cancer as a Cause of Resistance to Tyrosine Kinase Inhibitors.

Cancers (Basel) 2021 Apr 22;13(9). Epub 2021 Apr 22.

Department of Advanced Biomedical Sciences, University "Federico II", 80131 Naples, Italy.

Notch1 plays a key role in epithelial-mesenchymal transition (EMT) and in the maintenance of cancer stem cells. In the present study we tested whether high levels of activated Notch1 in oncogene-driven NSCLC can induce a reversible shift of driver dependence from EGFR to Notch1, and thus causing resistance to EGFR inhibitors. Adherent cells (parental) and tumor spheres (TS) from NSCLC H1975 cells and patient-derived CD133-positive cells were tested for EGFR and Notch1 signaling cascade. Read More

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Expression Profile of Fibroblast Growth Factor Receptors, Keratinocyte Differentiation Markers, and Epithelial Mesenchymal Transition-Related Genes in Actinic Keratosis: A Possible Predictive Factor for Malignant Progression?

Biology (Basel) 2021 Apr 15;10(4). Epub 2021 Apr 15.

Department of Clinical and Molecular Medicine, Sapienza University of Rome, 00189 Rome, Italy.

Actinic keratosis (AK) is the ultra violet (UV)-induced preneoplastic skin lesion clinically classified in low (KIN I), intermediate (KIN II), and high (KIN III) grade lesions. In this work we analyzed the expression of Fibroblast Growth Factor Receptors (FGFRs), as well as of keratinocyte differentiation and epithelial-to-mesenchymal transition (EMT)-related markers in differentially graded AK lesions, in order to identify specific expression profiles that could be predictive for direct progression of some KIN I lesions towards squamous cell carcinoma (SCC). Our molecular analysis showed that the keratinocyte differentiation markers keratin 1 (K1), desmoglein-1 (DSG1), and filaggrin (FIL) were progressively downregulated in KIN I, II, and III lesions, while the modulation of epithelial/mesenchymal markers and the induction of the transcription factors Snail1 and Zinc finger E-box-binding homeobox 1 (ZEB1) compatible with pathological EMT, even if observable, did not appear to correlate with AK progression. Read More

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Reprogramming Intestinal Epithelial Cell Polarity by Interleukin-22.

Front Med (Lausanne) 2021 12;8:656047. Epub 2021 Apr 12.

Department for Gastroenterology, Rheumatology and Infectious Diseases, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, Berlin, Germany.

Interleukin-22 (IL-22) impacts the integrity of intestinal epithelia and has been associated with the development of colitis-associated cancer and inflammatory bowel diseases (IBD). Previous data suggest that IL-22 protects the mucosal barrier and promotes wound healing and barrier defect. We hypothesized, that IL-22 modulates cell polarity of intestinal epithelial cells (IECs) acting on tight junction assembly. Read More

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Enhanced LINC01061 Levels as a Serum Biomarker in Gastric Cancer and Promotion of Malignant Transformation.

Oncol Res Treat 2021 28;44(5):242-251. Epub 2021 Apr 28.

Department of Laboratory Medicine, Nanjing Medical University Affiliated Suzhou Hospital North, Suzhou, China.

Background: The genomic copy number of LINC01061 is amplified in papillary thyroid cancer. However, its role in gastric cancer is not clear.

Materials And Methods: Tissues and serum of GC patients were collected to detect the expression of LINC01061 by quantitative real-time polymerase chain reaction (qRT-PCR). Read More

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Sema3C promotes hepatic metastasis and predicts poor prognosis in gastric adenocarcinoma.

J Int Med Res 2021 Apr;49(4):3000605211009802

Department of Gastrointestinal Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, P.R. China.

Objective: Semaphorin 3C (Sema3C) may regulate tumor metastasis and prognosis. We determined the biological roles of Sema3C in the hepatic metastasis of gastric adenocarcinoma and evaluated its clinical significance as a potential biomarker.

Methods: Sema3C expression in gastric cancer (GC) cell lines and tissues was measured using RT-qPCR and western blotting. Read More

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CDC20 promotes the progression of hepatocellular carcinoma by regulating epithelial‑mesenchymal transition.

Mol Med Rep 2021 Jul 28;24(1). Epub 2021 Apr 28.

Department of Hepatocellular Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637007, P.R. China.

Hepatocellular carcinoma (HCC) is a type of primary liver cancer, which is associated with high mortality. HCC is one of the most common malignant tumors worldwide. Cell division cycle 20 (CDC20) has been reported to be associated with the development of various malignant tumors and epithelial‑mesenchymal transition (EMT) has been reported to be involved in the malignant metastasis of HCC. Read More

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LncRNA LIPE-AS1 Predicts Poor Survival of Cervical Cancer and Promotes Its Proliferation and Migration via Modulating miR-195-5p/MAPK Pathway.

Front Oncol 2021 9;11:639980. Epub 2021 Apr 9.

Department of Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

A growing number of studies have unveiled that long non-coding RNA (lncRNA) is conductive to cervical cancer (CC) development. However, the effect of LIPE-AS1 is remained to be studied in CC. Reverse transcription-polymerase chain reaction (RT-PCR) was employed to measure LIPE-AS1 expression in CC tissues and the adjacent normal tissues. Read More

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TOX Acts as a Tumor Suppressor by Inhibiting mTOR Signaling in Colorectal Cancer.

Front Immunol 2021 9;12:647540. Epub 2021 Apr 9.

Department of Internal Oncology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

The treatment and prognosis of advanced colorectal cancer (CRC) remain a challenging clinical research focus. Here, we describe a new CRC tumor suppressor and potential therapeutic target: thymocyte selection associated high mobility group box (TOX) protein. The expression of TOX was lower in CRC than para-CRC. Read More

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CD26 as a target against fibrous formation in chronic airway rejection lesions.

Life Sci 2021 Apr 21;278:119496. Epub 2021 Apr 21.

Department of Thoracic Surgery, University Hospital Zurich, Zurich, Switzerland.

Aims: Chronic lung allograft dysfunction (CLAD) after lung transplantation (Tx) is the clinical result of chronic airway rejection lesions (CARL), histomorphologically described as either obliterative remodeling of small airways or alveolar fibroelastosis, or as a combination of both. We here investigated the CD26-inhibitory effect on CD26-expressing CARL.

Main Methods: CARL were induced by BALB/c → C57BL/6 mouse Tx under mild immunosuppression. Read More

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Propofol enhanced the cell sensitivity to paclitaxel (PTX) in prostatic cancer (PC) through modulation of HOTAIR.

Genes Genomics 2021 Apr 23. Epub 2021 Apr 23.

Department of Anesthesiology, West China Hospital, Sichuan University, No. 38, Guoxue Ave, Wuhou, Chengdu, Sichuan, 610041, China.

Background: PTX is widely used in cancer treatments.

Objective: In this paper, we explored the role and potential molecular mechanism of propofol in regulating PTX sensitivity in PC cells.

Methods: Prostatic cancer cell line PC3 was treated using different concentrations of PTX (10 nM, 50 nM), propofol (150 μM, 300 μM) or transfected with overexpressed HOTAIR plasmid. Read More

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COL4A1, negatively regulated by XPD and miR-29a-3p, promotes cell proliferation, migration, invasion and epithelial-mesenchymal transition in liver cancer cells.

H Zhang Y Wang H Ding

Clin Transl Oncol 2021 Apr 23. Epub 2021 Apr 23.

Department of Gastroenterology, The Second Affiliated Hospital of Nanchang University, Nanchang, 330006, Jiangxi, China.

Objective: Collagen type IV alpha 1 (COL4A1) exerts tumor-promoting functions in several tumors. However, its role in liver cancer remains not fully understood. Hence, this study aims to investigate the role of COL4A1 in regulating liver cancer cell behaviors and to validate its upstream regulatory mechanism. Read More

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Understanding the effect of mechanical forces on ovarian cancer progression.

Gynecol Oncol 2021 Apr 19. Epub 2021 Apr 19.

Department of Obstetrics and Gynecology, University of Alabama at Birmingham, Birmingham, AL 35233, USA. Electronic address:

Objective: Mechanical forces including tension, compression, and shear stress are increasingly implicated in tumor progression and metastasis. Understanding the mechanisms behind epithelial ovarian cancer (EOC) progression and metastasis is critical, and this study aimed to elucidate the effect of oscillatory and constant tension on EOC.

Methods: SKOV-3 and OVCAR-8 EOC cell lines were placed under oscillatory tension for 3 days and compared to cells placed under no tension. Read More

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Glucosamine attenuates drug resistance in Mitoxantrone-resistance breast cancer cells.

J Pharm Pharmacol 2021 Apr 22. Epub 2021 Apr 22.

Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad,Iran.

Objectives: This study was aimed at investigating the cytotoxicity and multi-drug resistance (MDR) reversal effect of Glucosamine (GlcN) on resistant BCRP-overexpressing breast cancer MCF-7/MX cells.

Methods: After confirming the overexpression of BCRP, the cytotoxicity and MDR reversing potential of GlcN on MCF-7/MX mitoxantrone-resistant and MCF-7 sensitive breast cancer cells were assessed via MTT assay. The effects of GlcN on mitoxantrone accumulation were analyzed through flow cytometry. Read More

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SPOCK1 promotes the proliferation and migration of colon cancer cells by regulating the NF-κB pathway and inducing EMT.

Neoplasma 2021 Apr 23. Epub 2021 Apr 23.

Department of Clinical Laboratory, The Third People's Hospital of Shenzhen, Southern University of Science and Technology, National Clinical Research Center for Infectious Diseases, Shenzhen, China.

Sparc/osteonectin, cwcv, and kazal-like domains proteoglycan 1 (SPOCK1) has been shown to promote various tumors, but its role in colon cancer (CRC) has not been clearly illuminated. The aim of this study was to investigate the effects of SPOCK1 interference on the proliferation, migration and EMT of CRC cells. First, we analyzed the expression of SPOCK1 in various CRC datasets. Read More

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