33,959 results match your criteria embryos decreased


Early suppression of the endocannabinoid degrading enzymes FAAH and MAGL alters locomotor development in zebrafish.

J Exp Biol 2021 08 26;224(16). Epub 2021 Aug 26.

Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada.

The fatty acid amide hydrolase (FAAH) and monoacylglycerol lipase (MAGL) enzymes are the predominant catabolic regulators of the major endocannabinoids (eCBs) anadamide (AEA) and 2-arachidonoylglycerol (2-AG), respectively. The expression and roles of eCBs during early embryogenesis remain to be fully investigated. Here, we inhibited FAAH and MAGL in zebrafish embryos during the first 24 h of life and examined motor neuron and locomotor development at 2 and 5 days post fertilization (dpf). Read More

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Different CO settings (6.0% vs 7.0%) do have an impact on extracellular pH of culture medium (pHe) and euploidy rates rather than on blastocyst development: a sibling oocyte study.

J Assist Reprod Genet 2021 Aug 26. Epub 2021 Aug 26.

ART Fertility Clinics, Abu Dhabi, United Arab Emirates.

Objective: To determine whether euploidy rates and blastocyst development differ in a continuous culture medium under different CO concentrations.

Design And Method: A single-center retrospective study was performed from July 2018 to October 2019 including 44 fresh cycles with at least four fresh mature oocytes (MII) without severe male factor infertility. Sibling MII were injected and cultured in Global®Total®LP under 6. Read More

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Inhibitory effects of Prorocentrum donghaiense allelochemicals on Sargassum fusiformis zygotes probed by JIP-test based on fast chlorophyll fluorescence kinetics.

Mar Environ Res 2021 Aug 20;170:105453. Epub 2021 Aug 20.

Zhejiang Provincial Key Laboratory for Subtropical Water Environment and Marine Biological Resources Protection, Wenzhou University, Wenzhou, 325035, China; National and Local Joint Engineering Research Center of Ecological Treatment Technology for Urban Water Pollution, Wenzhou University, Wenzhou, 325035, China. Electronic address:

The macro- and microalgae have been found to inhibit the growth and photosynthesis of one another due to allelopathic interactions between them. Sargassum fusiformis is a common and commercially cultivated seaweed in coastal waters of the East China Sea (ECS) and usually encounters dense harmful algal blooms (HABs) formed by dinoflagellates during their sexual reproduction period. In the present study, the effects of Prorocentrum donghaiense lipophilic extracted allelochemicals on the growth and photosynthesis of S. Read More

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In vitro teratogenicity testing using a 3D, embryo-like gastruloid system.

Reprod Toxicol 2021 Aug 20;105:72-90. Epub 2021 Aug 20.

Department of Genetics, University of Cambridge, Cambridge, UK. Electronic address:

Pharmaceuticals intended for use in patients of childbearing potential need to be tested for teratogenicity before marketing. Several pharmaceutical companies use animal-free in vitro models which allow a more rapid selection of lead compounds and contribute to 3Rs principles ('replace, reduce and refine') by streamlining the selection of promising compounds submitted to further regulatory studies in animals. Currently available in vitro models typically rely on adherent monolayer cultures or disorganized 3D structures, both of which lack the spatiotemporal and morphological context of the developing embryo. Read More

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Transcriptomes and DNA methylomes in apomictic cells delineate nucellar embryogenesis initiation in citrus.

DNA Res 2021 Aug 23. Epub 2021 Aug 23.

Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China.

Citrus nucellar poly-embryony (NPE) is a mode of sporophytic apomixis (SA) that asexual embryos formed in the seed through adventitious embryogenesis from the somatic nucellar cells. NPE allows clonal propagation of rootstocks, but it impedes citrus cross breeding. To understand the cellular processes involved in NPE initiation, we profiled the transcriptomes and DNA methylomes in laser microdissection (LMD) captured citrus apomictic cells. Read More

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The Heparan Sulfate Binding Peptide in Tumor Progression of Triple-Negative Breast Cancer.

Front Oncol 2021 4;11:697626. Epub 2021 Aug 4.

Department of Biochemistry/Molecular Biology, Universidade Federal de São Paulo, São Paulo, Brazil.

Angiogenesis is the formation of new vessels from pre-existing vasculature. The heparan sulfate chains from endothelial cell proteoglycans interact with the major angiogenic factors, regulating blood vessels´ formation. Since the FDA´s first approval, anti-angiogenic therapy has shown tumor progression inhibition and increased patient survival. Read More

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Domestic cat embryos generated without zona pellucida are capable of developing in vitro but exhibit abnormal gene expression and a decreased implantation rate.

Theriogenology 2021 Oct 12;174:36-46. Epub 2021 Aug 12.

Department of Animal Science, Faculty of Veterinary Sciences, Universidad de Concepcion, Chillán, Chile.

The removal of the zona pellucida has been used to improve the in vitro development of domestic cat embryos generated by IVF and SCNT. However, the in vivo development of domestic cat embryos generated without the zona pellucida has not been evaluated. The objective of this study was to evaluate the effects of zona pellucida removal on the in vitro and in vivo development of domestic cat embryos generated by IVF. Read More

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October 2021

Effects of sublethal concentration of metamifop on hepatic lipid metabolism in adult zebrafish (Danio rerio).

Aquat Toxicol 2021 Aug 11;238:105938. Epub 2021 Aug 11.

Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China. Electronic address:

Metamifop (MET) is an effective herbicide that has been extensively used in paddy fields. Previous research demonstrated that MET was highly toxic to zebrafish embryos, and this threat has caused great concern; moreover, 0.40 mg/L MET elevated the hepatosomatic index (HSI) in adult zebrafish without lethal effect after 21 d of exposure. Read More

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Adverse effects of bisphenol B exposure on the thyroid and nervous system in early life stages of zebrafish.

Comp Biochem Physiol C Toxicol Pharmacol 2021 Aug 16;250:109167. Epub 2021 Aug 16.

School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China; Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China. Electronic address:

Bisphenol B (BPB), a widely used alternative of bisphenol A (BPA), has been detected in various environmental media and foodstuffs. However, the knowledge of the health risks about BPB is still limited. In this study, the effects of BPB on thyroid hormone homeostasis and neuronal development were evaluated by exposure of embryos 2 h post-fertilization (hpf) to BPB (0, 1, 10, 100 and 1000 μg/L) until 144 hpf. Read More

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Modulation of lipid metabolism through multiple pathways during oocyte maturation and embryo culture in bovine.

Zygote 2021 Aug 18:1-9. Epub 2021 Aug 18.

Animal Reproduction Laboratory, Embrapa Dairy Cattle, 610 Eugenio do Nascimento Ave., Juiz de Fora, MG, Brazil.

Lipid accumulation occurs in cultured embryos and is associated with reduced cryotolerance. Here we report the use of a multiple pathway lipid modulator cocktail (l-carnitine, linoleic acid and forskolin) to improve cryosurvival. First, we stained oocytes and embryos with Oil Red to examine the time course of lipid accumulation during in vitro fertilization (IVF) and embryo culture. Read More

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Nuclear-localized eukaryotic translation initiation factor 1A is involved in mouse preimplantation embryo development.

J Mol Histol 2021 Aug 17. Epub 2021 Aug 17.

Key Laboratory of Stem Cell Engineering and Regenerative Medicine of Fujian Province University, Fujian Medical University, Fuzhou, 350122, People's Republic of China.

Preimplantation embryo development is characterized by drastic nuclear reprogramming and dynamic stage-specific gene expression. Key regulators of this earliest developmental stage have not been revealed. In the present study, a "non-classical" nuclear-localization pattern of eIF1A was observed during early developmental stages of mouse preimplantation embryo before late-morula. Read More

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A sublethal ATP11A mutation associated with neurological deterioration causes aberrant phosphatidylcholine flipping in plasma membranes.

J Clin Invest 2021 Sep;131(18)

Laboratory of Biochemistry and Immunology, World Premier International Research Center, Immunology Frontier Research Center, Osaka University, Suita, Osaka, Japan.

ATP11A translocates phosphatidylserine (PtdSer), but not phosphatidylcholine (PtdCho), from the outer to the inner leaflet of plasma membranes, thereby maintaining the asymmetric distribution of PtdSer. Here, we detected a de novo heterozygous point mutation of ATP11A in a patient with developmental delays and neurological deterioration. Mice carrying the corresponding mutation died perinatally of neurological disorders. Read More

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September 2021

Investigation of the Oxidative Stress Response of a Green Synthesis Nanoparticle (RP-Ag/ACNPs) in Zebrafish.

Biol Trace Elem Res 2021 Aug 17. Epub 2021 Aug 17.

Department of Seafood Processing Technology, Faculty of Fisheries, Ataturk University, TR-25030, Erzurum, Turkey.

Silver nanoparticles (AgNPs) are prominent nanomaterials that are efficiently used in different industries including medical products, water treatment, and cosmetics. However, AgNPs are known to cause adverse effects on the ecosystem and human health. In this study, aqueous extract of Rumex patientia (RP) was used as a reducing and stabilizing agent in AgNP biosynthesis. Read More

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Zinc alleviates the heat stress of primary cultured hepatocytes of broiler embryos via enhancing the antioxidant ability and attenuating the heat shock responses.

Anim Nutr 2021 Sep 24;7(3):621-630. Epub 2021 Apr 24.

Poultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.

Zinc (Zn) has been shown to attenuate the adverse effects of heat stress on broilers, but the mechanisms involving this process remain unclear. We aimed to investigate possible protective mechanisms of Zn on primary cultured hepatocytes of broiler embryos subjected to heat stress. Three experiments were conducted. Read More

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September 2021

Contrast-enhanced ultrasound evaluation of placental perfusion in brachicephalic bitches.

Theriogenology 2021 Oct 10;173:230-240. Epub 2021 Aug 10.

School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp), Jaboticabal, São Paulo, Brazil; Diagnostic Imaging Service, Department of Large Animal Clinic, Federal University of Santa Maria (UFSM), Santa Maria, Rio Grande do Sul, Brazil. Electronic address:

The present study aimed to investigate placental hemodynamics to determine quantitative and qualitative parameters for pregnant brachycephalic bitches as well as describe placental vascularization and perfusion in females with fetal abnormalities close to delivery. Forty-four healthy fetuses from 22 brachycephalic bitches and 9 fetuses with gestational abnormalities (anasarca and hydrocephalus) from 8 brachycephalic bitches were evaluated. All female dogs were artificially inseminated intravaginally and underwent cesarean section at the end of gestation. Read More

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October 2021

CRISPR/Cas9-Induced Loss-of-Function Mutation in the Barley Gene Causes Abnormal Embryo Development Leading to Severely Reduced Grain Germination and Seedling Shootless Phenotype.

Front Plant Sci 2021 30;12:670302. Epub 2021 Jul 30.

Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Cell Biology, Faculty of Science, Palacký University Olomouc, Olomouc, Czechia.

The diverse roles of mitogen-activated protein kinases (MAPKs, MPKs) in plant development could be efficiently revealed by reverse genetic studies. In knockout mutants complete the life cycle; however, ~40% of their embryos show defects in the development leading to abnormal phenotypes of seeds and seedlings' roots. Contrary to the , the rice () is an essential gene as transfer DNA (T-DNA) insertion and CRISPR/Cas9 induced loss-of-function mutations in the cause early embryo arrest. Read More

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Sox9CreER-mediated deletion of β-catenin in palatal mesenchyme results in delayed palatal elevation accompanied with repressed canonical Wnt signaling and reduced actin polymerization.

Genesis 2021 Sep 13;59(9):e23441. Epub 2021 Aug 13.

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Cleft Lip and Palate Surgery, West China School of Stomatology, Sichuan University, Chengdu, China.

Cleft palate is a good model to pushing us toward a deeper understanding of the molecular mechanisms of spatiotemporal patterns in tissues and organisms because of the multiple-step processes such as elevation and fusion. Previous studies have shown that the epithelial β-catenin is crucial for palatal fusion, however, the function of the mesenchymal β-catenin remains elusive. We investigate the role of mesenchymal β-catenin in palatal development by generating a β-catenin conditional knockout mouse (CKO) (Sox9CreER; Ctnnb1 ). Read More

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September 2021

Melatonin alters bovine uterine artery hemodynamics, vaginal temperatures, and fetal morphometrics during late gestational nutrient restriction in a season-dependent manner.

J Anim Sci 2021 Sep;99(9)

Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, MS 39762, USA.

The objectives were to examine melatonin-mediated changes in temporal uterine blood flow (UBF), vaginal temperatures (VTs), and fetal morphometrics in 54 commercial Brangus heifers (Fall, n = 29; Summer, n = 25) during compromised pregnancy. At day 160 of gestation, heifers were assigned to one of the four treatments consisting of adequately fed (ADQ-CON; 100% National Research Council [NRC]; n = 13), global nutrient restricted (RES-CON; 60% NRC; n =13), and ADQ or RES supplemented with 20 mg/d of melatonin (ADQ-MEL, n = 13; RES-MEL, n = 15). In the morning (0500 hours; AM) and afternoon (1300 hours; PM) of day 220 of gestation, UBF was determined via Doppler ultrasonography, while temperature data loggers attached to progesterone-free controlled internal drug releases were used to record VTs. Read More

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September 2021

Histone deacetylase inhibition leads to regulatory histone mark alterations and impairs meiosis in oocytes.

Epigenetics Chromatin 2021 Aug 12;14(1):39. Epub 2021 Aug 12.

Univ. Rennes, EHESP, Inserm, Irset (Institut de Recherche en Santé, environnement et travail) - UMR_S 1085, 35000, Rennes, France.

Background: Panobinostat (PB), a histone deacetylase (HDAC) inhibitor drug, is clinically used in the treatment of cancers. We investigated the effects of PB on murine ovarian functions in embryos and adult animals.

Methods: C57BL/6J mice were treated with 5 mg/kg PB on alternate days from embryonic day (E) 6. Read More

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Application of antagonist regimen in patients with failed pregnancy assisted by previous long-term regimen during early follicular phase.

Am J Transl Res 2021 15;13(7):8365-8371. Epub 2021 Jul 15.

Department of Reproduction and Genetics, Ganzhou Maternal and Child Health Centre Ganzhou, Jiangxi Province, China.

Objective: To investigate the clinical application of gonadotropin-releasing hormone antagonist (GnRH-ant) in patients with failed pregnancy assisted by the previous long-term regimen during early follicular phase (EFP).

Methods: A total of 122 patients with good ovarian function and two previous failed EFP long-term assisted pregnancy were selected from the reproductive center of our hospital for study. All patients were assisted by in vitro fertilization-embryo transfer (IVF-ET) twice. Read More

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The transcription factor CmLEC1 positively regulates the seed-setting rate in hybridization breeding of chrysanthemum.

Hortic Res 2021 Aug 10;8(1):191. Epub 2021 Aug 10.

College of Horticulture, Nanjing Agricultural University, Key Laboratory of Landscape Design, Ministry of Agriculture and Rural Affairs, 210095, Nanjing, China.

Distant hybridization is widely used to develop crop cultivars, whereas the hybridization process of embryo abortion often severely reduces the sought-after breeding effect. The LEAFY COTYLEDON1 (LEC1) gene has been extensively investigated as a central regulator of seed development, but it is far less studied in crop hybridization breeding. Here we investigated the function and regulation mechanism of CmLEC1 from Chrysanthemum morifolium during its seed development in chrysanthemum hybridization. Read More

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Cardiovascular toxicity assessment of polyethylene nanoplastics on developing zebrafish embryos.

Chemosphere 2021 Nov 8;282:131124. Epub 2021 Jun 8.

Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, 100069, PR China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, 100069, PR China. Electronic address:

Environmental exposure to nanoplastics is inevitable as the application of nanoplastics in our daily life is more and more extensively. So, the adverse effects of nanoplastics on human health are also gaining greater concerns. However, the subsequent toxicological response to nanoplastics, especially on cardiovascular damage was still largely unknown. Read More

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November 2021

Determining Diagnostic Criteria of Unexplained Recurrent Implantation Failure: A Retrospective Study of Two Three or More Implantation Failure.

Front Endocrinol (Lausanne) 2021 22;12:619437. Epub 2021 Jul 22.

Center for Reproductive Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Background: The definition of recurrent implantation failure (RIF) differs clinically, one of the most controversial diagnostic criteria is the number of failed treatment cycles. We tried to investigate whether the two implantation failure could be included in the diagnostic criteria of RIF.

Methods: A retrospective analysis of the clinical data of patients (N=1518) aged under 40 years with two or more implantation failure, recruited from the Center for Reproductive Medicine of the First Affiliated Hospital of Zhengzhou University from January 2016 to June 2019. Read More

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Toxicity of boron and vanadium nanoparticles on Danio rerio embryos - Phenotypical, biochemical, and behavioral alterations.

Aquat Toxicol 2021 Jul 31;238:105930. Epub 2021 Jul 31.

Department of Biology & CESAM, University of Aveiro, 3810-193 Aveiro, Portugal. Electronic address:

Engineered nanoparticles (NPs) are emerging contaminants of concern and it is important to understand their environmental behavior and ecological risks to exposed organisms. Despite their ubiquitous presence in the environment, there is little information about the hazards of certain NPs, such as boron (BNPs) and vanadium (VNPs). The aim of the present research was to investigate the effects of commercial BNPs and VNPs (80 to 100 nm) to zebrafish embryos, at different levels of biological organization. Read More

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Clinical and imaging outcomes after intrathecal injection of umbilical cord tissue mesenchymal stem cells in cerebral palsy: a randomized double-blind sham-controlled clinical trial.

Stem Cell Res Ther 2021 08 6;12(1):439. Epub 2021 Aug 6.

Pediatrics Center of Excellence, Department of Pediatric Neurology, Children's Medical Center, Growth and Development Research Center, Tehran University of Medical Sciences, Tehran, Iran.

Background: This study assessed the safety and efficacy of intrathecal injection of umbilical cord tissue mesenchymal stem cells (UCT-MSC) in individuals with cerebral palsy (CP). The diffusion tensor imaging (DTI) was performed to evaluate the alterations in white-matter integrity.

Methods: Participants (4-14 years old) with spastic CP were assigned in 1:1 ratio to receive either UCT-MSC or sham procedure. Read More

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Human Placental Transcriptome Reveals Critical Alterations in Inflammation and Energy Metabolism with Fetal Sex Differences in Spontaneous Preterm Birth.

Int J Mol Sci 2021 Jul 23;22(15). Epub 2021 Jul 23.

Center for Research on Reproduction and Women's Health, Department of Obstetrics and Gynecology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

A well-functioning placenta is crucial for normal gestation and regulates the nutrient, gas, and waste exchanges between the maternal and fetal circulations and is an important endocrine organ producing hormones that regulate both the maternal and fetal physiologies during pregnancy. Placental insufficiency is implicated in spontaneous preterm birth (SPTB). We proposed that deficits in the capacity of the placenta to maintain bioenergetic and metabolic stability during pregnancy may ultimately result in SPTB. Read More

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Research Note: Effects of glycerol monolaurate supplementation on egg production, biochemical indices, and gut microbiota of broiler breeders at the late stage of production.

Poult Sci 2021 Sep 14;100(9):101386. Epub 2021 Jul 14.

School of Life Science and Engineering, Foshan University, Foshan 528000, China. Electronic address:

The objective of the present study was to determine the effect of glycerol monolaurate (GML) supplementation on egg production, biochemical indices, and gut microbiota of broiler breeders at the late stage of production. Total of 180 healthy Qingyuan Partridge broiler breeders were randomly assigned to 2 groups: 1) corn-soybean meal based diet, and 2) basal diet supplemented with 300 mg glycerol monolaurate/kg. Each treatment group had 6 replicates with 15 birds within each replicate. Read More

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September 2021

Entry of antiepileptic drugs (valproate and lamotrigine) into the developing rat brain.

F1000Res 2021 13;10:384. Epub 2021 May 13.

Biochemistry & Pharmacology, University of Melbourne, Parkville, Victoria, 3010, Australia.

Women with epilepsy face difficult choices whether to continue antiepileptic drug treatment during pregnancy, as uncontrolled seizures carry great risk to mother and fetus but continuing treatment may have adverse effects on baby's development. This study aimed at evaluating antiepileptic drug entry into developing brain. Anaesthetised pregnant, non-pregnant adult females, postnatal and fetal rats were injected intraperitoneally with different doses, single or in combinations, of valproate and lamotrigine, within clinical range. Read More

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Effect of lgals3a on embryo development of zebrafish.

Transgenic Res 2021 Aug 4. Epub 2021 Aug 4.

Department of Cardiology, Shanghai Ninth People's Hospital Affiliated Shanghai Jiaotong University School of Medicine, No. 639 Zhizaoju Road, Shanghai, 200011, China.

Our study was aimed to investigate the effects of lgals3a (Gal-3 encoding gene) on the development of zebrafish embryo and its underlying mechanisms. Morpholino (MO) technology was used to inhibit the expression of zebrafish lgals3a, and the effect of lgals3a gene knockdown on zebrafish embryo development and the number of monocyte macrophages was observed. Effect of lgals3a-e3i3-MO on apoptosis of zebrafish was detected by acridine orange staining. Read More

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BPA and BPS affect the expression of anti-Mullerian hormone (AMH) and its receptor during bovine oocyte maturation and early embryo development.

Reprod Biol Endocrinol 2021 Aug 3;19(1):119. Epub 2021 Aug 3.

Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.

Background: Exposure to endocrine-disrupting chemicals, such as Bisphenol A (BPA) and Bisphenol S (BPS), is widespread and has negative implications on embryonic development. Preliminary evidence revealed that in women undergoing IVF treatment, urinary BPA levels were associated with low serum anti-Mullerian hormone, however a definitive relationship between the two has not yet been characterized.

Methods: This study aimed to evaluate BPA and BPS effects on in vitro oocyte maturation and early preimplantation embryo development through i) analysis of anti-Mullerian hormone (AMH) and anti-Mullerian hormone receptor II (AMHRII), ii) investigation of developmental parameters, such as cleavage, blastocyst rates and developmental arrest, iii) detection of apoptosis and iv) assessment of possible sex ratio skew. Read More

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