21,100 results match your criteria embryo implantation

Strategies for managing asherman's syndrome and endometrial atrophy: Since the classical experimental models to the new bioengineering approach.

Mol Reprod Dev 2021 Jul 22. Epub 2021 Jul 22.

Fundación Instituto Valenciano de Infertilidad (FIVI), La Fe Health Research Institute, Valencia, Spain.

Endometrial function is essential for embryo implantation and pregnancy, but managing endometrial thickness that is too thin to support pregnancy or an endometrium of compromised functionality due to intrauterine adhesions is an ongoing challenge in reproductive medicine. Here, we review current and emerging therapeutic and experimental options for endometrial regeneration with a focus on animal models used to study solutions for Asherman's syndrome and endometrial atrophy, which both involve a damaged endometrium. A review of existing literature was performed that confirmed the lack of consensus on endometrial therapeutic options, though promising new alternatives have emerged in recent years (platelet-rich plasma, exosomes derived from stem cells, bioengineering-based techniques, endometrial organoids, among others). Read More

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Signaling through retinoic acid receptors is essential for mammalian uterine receptivity and decidualization.

JCI Insight 2021 Jul 22. Epub 2021 Jul 22.

Division of Dermatology, Department of Medicine, Washington University School of Medicine, St. Louis, United States of America.

Retinoic Acid (RA) signaling has long been speculated to regulate embryo implantation, because many enzymes and proteins responsible for maintaining RA homeostasis and transducing RA signals are tightly regulated in the endometrium during this critical period. However, due to lack of genetic data, it was unclear whether RA signaling is truly required for implantation, and which specific RA signaling cascades are at play. Herein we utilize a genetic murine model that expresses a dominant negative form of RA receptor specifically in female reproductive organs to show that functional RA signaling is fundamental to female fertility, particularly implantation and decidualization. Read More

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Conservative cesarean scar pregnancy treatment: local methotrexate injection followed by hysteroscopic removal with hysteroscopic tissue removal system.

Fertil Steril 2021 Jul 18. Epub 2021 Jul 18.

Division of Gynecology, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy.

Objective: To describe a conservative cesarean scar pregnancy (CSP) treatment in a patient with the desire for future pregnancy. To date, there is no gold standard for the management of a viable CSP. There is a wide range of treatment options that include major surgery and minimally invasive procedures. Read More

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Trophoblast derived extracellular vesicles specifically alter the transcriptome of endometrial cells and may constitute a critical component of embryo-maternal communication.

Reprod Biol Endocrinol 2021 Jul 21;19(1):115. Epub 2021 Jul 21.

Department of Pathophysiology, Institute of Biomedicine and Translational Medicine, Faculty of Medicine, Tartu University, Tartu, Estonia.

Background: The period of time when the embryo and the endometrium undergo significant morphological alterations to facilitate a successful implantation-known as "window of implantation"-is a critical moment in human reproduction. Embryo and the endometrium communicate extensively during this period, and lipid bilayer bound nanoscale extracellular vesicles (EVs) are purported to be integral to this communication.

Methods: To investigate the nature of the EV-mediated embryo-maternal communication, we have supplemented trophoblast analogue spheroid (JAr) derived EVs to an endometrial analogue (RL 95-2) cell layer and characterized the transcriptomic alterations using RNA sequencing. Read More

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Post-thaw culture has no beneficial effect on frozen embryos transfer outcomes comparing to same embryonal age non-cultured frozen-thawed embryos.

J Matern Fetal Neonatal Med 2021 Jul 21:1-8. Epub 2021 Jul 21.

Fertility and IVF Unit, Soroka University Medical Center, Ben-Gurion University of the Negev, Beer-Sheva, Israel.

Purpose: To assess the impact of post-thawing embryo culture on frozen embryo transfer (FET) outcomes.

Methods: A retrospective cohort study including 678 consecutive FET cycles performed between the years 2004 and 2017 was conducted. Patients older than 45 years old were excluded. Read More

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Neutrophils in pregnancy: new insights into innate and adaptive immune regulation.

Immunology 2021 Jul 20. Epub 2021 Jul 20.

William Harvey Research Institute, Barts and the London School of Medicine Queen Mary University.

The immunology of pregnancy has been the focus of many studies to better understand how the mother is able to tolerate the presence of a semi-allogeneic foetus. Far from the initial view of pregnancy as a state of immunosuppression, successful foetal development from implantation to birth, is now known to be under the control of an intricate balance of immune cells. The balance between pro-inflammatory functions used to promote embryo implantation and placental development and immunosuppressive activity to maintain maternal tolerance of the foetus, is an immunological phenotype unique to pregnancy which is dependent on the time of gestation. Read More

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Association between redundant endometrium and endometrial polyps: a pilot study.

Minerva Obstet Gynecol 2021 Jul 20. Epub 2021 Jul 20.

Department of Obstetrics and Gynecology, C.S. Mott Center for Human Development, Wayne State University, Detroit, MI, USA.

Objective: To explore the organic features of redundant endometrium (RE), we examined the expression of different endometrial hormone receptors, oncogenes, and cell replication markers, in normal endometrium (NE), endometrial polyps (EP) and RE specimens.

Methods: This was an experimental study examining endometrial tissue expression of estrogen receptors (ER1 and 2), progesterone receptors (PR-A+B), androgen receptor (AR), insulin receptor (Insulin-R), insulin-like growth factor receptor 1 (IGFR-1), thyroid hormone receptor (TH-RB), B-cell lymphoma 2 (Bcl-2), Ki67, HOXA10, in women with NE, EP and RE, of women undergoing hysteroscopy for benign gynecologic pathology. Specimens were separated in 3 groups: NE, EP, RE. Read More

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The effect of endometrial scratch on pregnancy outcomes of frozen-thawed embryo transfer: a propensity score-matched study.

Xiao Han Linli Hu

Gynecol Endocrinol 2021 Jul 19:1-6. Epub 2021 Jul 19.

Reproductive Medical Center, Henan Province Key Laboratory of Reproduction and Genetics,The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Aims: To study the effect of endometrial scratch (ES) performed on Days 2 to 5 of the menstrual cycle on pregnancy outcomes of frozen-thawed embryo transfer (FET).

Materials And Methods: We conducted a retrospective study including 5769 women undergoing FET from January 2018 to August 2019. The endometrial scratching group (group ES) consisted of 827 patients and the control group (group non-ES) comprised 4942 patients. Read More

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The effects of aging on molecular modulators of human embryo implantation.

iScience 2021 Jul 19;24(7):102751. Epub 2021 Jun 19.

Discovery and Translational Science Department, Leeds Institute of Cardiovascular and Metabolic Medicine, University of Leeds, Leeds, LS2 9JT, UK.

Advancing age has a negative impact on female fertility. As implantation rates decline during the normal maternal life course, age-related, embryonic factors are altered and our inability to monitor these factors in an unbiased genome-wide manner has severely limited our understanding of early human embryo development and implantation. Our high-throughput methodology uses trophectoderm samples representing the full spectrum of maternal reproductive ages with embryo implantation potential examined in relation to trophectoderm transcriptome dynamics and reproductive maternal age. Read More

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Granulocyte-Macrophage Colony Stimulating Factor in Single Blastocyst Conditioned Medium as a Biomarker for Predicting Implantation Outcome of Embryo.

Front Immunol 2021 30;12:679839. Epub 2021 Jun 30.

Shenzhen Key Laboratory of Reproductive Immunology for Peri-Implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, China.

Background: It is highly desirable to develop new strategies based on secretomics to more accurately selection of embryos with the highest developmental potential for transfer. Granulocyte-macrophage colony-stimulating factor (GM-CSF) has been reported to promote embryo development and pregnancy establishment. However, the predictive value of GM-CSF in single blastocyst selection remains unclear. Read More

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Optimizing the Expression and Solubilization of an E. coli-Produced Leukemia Inhibitory Factor for Anti-LIF Antibody Production and Use Thereof for Contraception in Mice.

Mol Biotechnol 2021 Jul 16. Epub 2021 Jul 16.

Animal Biotechnology Group, Department of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.

Leukemia inhibitory factor (LIF) is an essential cytokine for blastocyst implantation. This study evaluated the effect of LIF inhibition on the blockage of embryo implantation. A truncated mouse LIF (tmLIF) was designed and expressed in E. Read More

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Podocalyxin inhibits human embryo implantation in vitro and luminal podocalyxin in putative receptive endometrium is associated with implantation failure in fertility treatment.

Fertil Steril 2021 Jul 13. Epub 2021 Jul 13.

School of Health and Biomedical Sciences, Royal Melbourne Institute of Technology University, Victoria, Australia; Hudson Institute of Medical Research, Clayton, Victoria, Australia. Electronic address:

Objective: To study whether endometrial epithelial podocalyxin (PCX) inhibits implantation of human embryos in vitro and in patients undergoing in vitro fertilization (IVF).

Design: We have recently identified PCX as a key negative regulator of endometrial epithelial receptivity. Podocalyxin is expressed in all epithelial cells in the nonreceptive endometrium, but is selectively downregulated in the luminal epithelium (LE) for receptivity. Read More

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Tumor susceptibility gene 101 is required for the maintenance of uterine epithelial cells during embryo implantation.

Reprod Biol Endocrinol 2021 Jul 16;19(1):112. Epub 2021 Jul 16.

Department of Veterinary Medicine, School of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea.

Background: The tumor susceptibility gene 101 (Tsg101), a component of the endosomal sorting complex required for transport (ESCRT) complex I, is involved in multiple biological processes involving endomembranous structures and the plasma membrane. The role of Tsg101 in the uterine epithelium was investigated in Tsg101 floxed mice crossed with Lactoferrin-iCre mice (Tsg101).

Methods: Tsg101 mice were bred with stud male mice and the status of pregnancy was examined on days 4 and 6. Read More

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Inconsistencies between endometrial receptivity assay (ERA) and Adhesio-RT test for the window of implantation in women with repetitive failed donor oocyte embryo transfers.

Obstet Gynecol Sci 2021 Jul 15. Epub 2021 Jul 15.

Department of Obstetrics and Gynecology, McGill University Health Centre Reproductive Centre, Montreal, Canada.

Two commercially available second-generation endometrial receptivity assays using microarray analysis or next-generation sequencing are available in the market: endometrial receptivity assay (ERA) (Igenomix Laboratories) and Adhesio RT (OVO Laboratories). Little is known about how the results of these tests compare. We present a case of a subject with repetitive failed donor oocyte embryo transfer, who underwent evaluation of endometrial receptivity using both the Adhesio and ERA tests. Read More

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MiR-191-5p is upregulated in culture media of implanted human embryo on day fifth of development.

Reprod Biol Endocrinol 2021 Jul 13;19(1):109. Epub 2021 Jul 13.

Department of Immunobioquímica, Instituto Nacional de Perinatología "Isidro Espinosa de los Reyes" (INPerIER), Ciudad de México, México.

Background: Morphological features are the most common criteria used to select human embryos for transfer to a receptive uterine cavity. However, such characteristics are not valid for embryos in cellular arrest. Even aneuploid embryos can have normal morphology, and some euploid embryos have aberrant morphology. Read More

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DDX21 is a p38-MAPK-sensitive nucleolar protein necessary for mouse preimplantation embryo development and cell-fate specification.

Open Biol 2021 Jul 14;11(7):210092. Epub 2021 Jul 14.

Laboratory of Early Mammalian Developmental Biology (LEMDB), Department of Molecular Biology and Genetics, Faculty of Science, University of South Bohemia, Branišovská 31, 37005 České Budějovice, Czech Republic.

Successful navigation of the mouse preimplantation stages of development, during which three distinct blastocyst lineages are derived, represents a prerequisite for continued development. We previously identified a role for p38-mitogen-activated kinases (p38-MAPK) regulating blastocyst inner cell mass (ICM) cell fate, specifically primitive endoderm (PrE) differentiation, that is intimately linked to rRNA precursor processing, polysome formation and protein translation regulation. Here, we develop this work by assaying the role of DEAD-box RNA helicase 21 (DDX21), a known regulator of rRNA processing, in the context of p38-MAPK regulation of preimplantation mouse embryo development. Read More

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Forced Suppression of let-7a-5p in Mouse Blastocysts Improves Implantation Rate.

Reprod Sci 2021 Jul 12. Epub 2021 Jul 12.

Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, 1477893855, Iran.

Embryo implantation requires appropriate communication between the blastocyst and endometrium. Recurrent implantation failure is an essential component of assisted reproductive technology. Also, miRNA-mediated gene expression impacts the implantation process, and the downregulation of some miRs, such as mmu-let-7a, improves this process. Read More

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Morphogenesis of the human preimplantation embryo: bringing mechanics to the clinics.

Semin Cell Dev Biol 2021 Jul 9. Epub 2021 Jul 9.

Institut Curie, PSL Research University, Sorbonne Université, CNRS UMR3215, INSERM, U934 Paris, France. Electronic address:

During preimplantation development, the human embryo forms the blastocyst, the structure enabling uterine implantation. The blastocyst consists of an epithelial envelope, the trophectoderm, encompassing a fluid-filled lumen, the blastocoel, and a cluster of pluripotent stem cells, the inner cell mass. This specific architecture is crucial for the implantation and further development of the human embryo. Read More

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SOX2 plays a crucial role in cell proliferation and lineage segregation during porcine pre-implantation embryo development.

Cell Prolif 2021 Jul 11:e13097. Epub 2021 Jul 11.

Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Sciences, Seoul National University, Gwanak-gu, Korea.

Objectives: Gene regulation in early embryos has been widely studied for a long time because lineage segregation gives rise to the formation of a pluripotent cell population, known as the inner cell mass (ICM), during pre-implantation embryo development. The extraordinarily longer pre-implantation embryo development in pigs leads to the distinct features of the pluripotency network compared with mice and humans. For these reasons, a comparative study using pre-implantation pig embryos would provide new insights into the mammalian pluripotency network and help to understand differences in the roles and networks of genes in pre-implantation embryos between species. Read More

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Investigating the impact of asymptomatic or mild SARS-CoV-2 infection on female fertility and fertilization outcomes: A retrospective cohort study.

EClinicalMedicine 2021 Aug 6;38:101013. Epub 2021 Jul 6.

Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

The current study aimed to investigate the impact of asymptomatic or mild severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection on female fertility and laboratory and clinical outcomes in assisted reproductive technology (ART) treatments. Patients undergoing ART treatments in the Reproductive Medicine Center, Tongji Hospital, Wuhan, from May 2020 to February 2021 were enrolled. Seventy of them were positive for serum SARS-CoV-2 antibodies (IgG and/or IgM), and 3973 patients had negative results. Read More

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Generation of Mouse Pluripotent Stem Cell-derived Trunk-like Structures: An Model of Post-implantation Embryogenesis.

Bio Protoc 2021 Jun 5;11(11):e4042. Epub 2021 Jun 5.

Dept. of Developmental Genetics, Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany.

Post-implantation mammalian embryogenesis involves profound molecular, cellular, and morphogenetic changes. The study of these highly dynamic processes is complicated by the limited accessibility of development. In recent years, several complementary systems comprising self-organized assemblies of mouse embryonic stem cells, such as gastruloids, have been reported. Read More

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GLUT4 in Mouse Endometrial Epithelium: Roles in Embryonic Development and Implantation.

Front Physiol 2021 25;12:674924. Epub 2021 Jun 25.

Department of Physiology, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, China.

GLUT4 is involved in rapid glucose uptake among various kinds of cells to contribute to glucose homeostasis. Prior data have reported that aberrant glucose metabolism by GLUT4 dysfunction in the uterus could be responsible for infertility and increased miscarriage. However, the expression and precise functions of GLUT4 in the endometrium under physiological conditions remain unknown or controversial. Read More

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Recent Judgement of the Italian Judiciary about medical assisted procreation (MAP): is informed consent valid after parents separation?

Clin Ter 2021 Jul;172(4):253-255

University of Siena, AGI medica, Siena, Italy.

Abstract: Law No 40/2004 regulates in Italy the matter of medically assisted procreation (MAP). Recently, the Tribunal of Capua Vetere expressed its position on the subject of informed consent in a case of MAP. In the specific case, a couple entered the preliminary stages of the PMA procedures, carrying out the fertilization of the ovum and the embryo production. Read More

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Hysteroscopic endometrial peeling as a different approach to endometrial scratching. Case series report.

J Gynecol Obstet Hum Reprod 2021 Jul 7;50(10):102195. Epub 2021 Jul 7.

Reproductive Medicine Associates on New York-international, Mexico, CDMX. Paseo de la Reforma 2693, Lomas de Bezares, 11910 Ciudad de México, CDMX, Mexico.

Study Question: Does hysteroscopic endometrial peeling improve reproductive outcomes in women with implantation failure?

Design: In this case series, 66 women underwent an hysteroscopic endometrial peeling and subsequently, a single frozen embryo transfer.

Results: After the exclusion of patients with intraoperative incidental findings, pregnancy was achieved in 63% (42/66) of the women who underwent hysteroscopic endometrial peeling and subsequently a single frozen embryo transfer, clinical pregnancy rate was observed in 57% (38/66) of the patients and, clinical pregnancy loss occurred in 10% (4/38).

Conclusion: this novel surgical technique seems to increase implantation as well as clinical pregnancy rates in women with repeated implantation failure. Read More

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Pre-Implantation Gender Selection: Family Balancing in Jordan.

Risk Manag Healthc Policy 2021 2;14:2797-2801. Epub 2021 Jul 2.

Department of Dermatology, Albalqa Applied University, Salt, Jordan.

Purpose: To find out whether couples with predominant female offspring have more chances to produce more female embryos during non-medical pre-implantation genetic diagnosis (PGD) for male sex selection.

Methods: A total of 125 couples who had three or more female offspring and underwent non-medical PGD for male sex selection between 2015 and 2019 were included. Nuclear DNA was analyzed by fluorescent in situ hybridization (FISH). Read More

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The long noncoding RNA MEG3 regulates Ras-MAPK pathway through RASA1 in trophoblast and is associated with unexplained recurrent spontaneous abortion.

Mol Med 2021 Jul 8;27(1):70. Epub 2021 Jul 8.

Department of Ophthalmology, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University), Shenzhen, 518020, People's Republic of China.

Background: Maternally Expressed Gene 3 (MEG3) is expressed at low levels in placental villi during preeclampsia; however, its roles in unexplained recurrent spontaneous abortion (URSA) remain unclear. In this study, we aimed to explore the relationship between MEG3 and URSA.

Methods: The differentially expressed lncRNAs (MEG3) and its downstream genes (RASA1) were identified using bioinformatics analysis of Genomic Spatial Event (GSE) database. Read More

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Preimplantation Genetic Testing for Aneuploidy: Current Perspectives.

Semin Reprod Med 2021 Jul 8. Epub 2021 Jul 8.

Centre for Reproductive and Genetic Health, London, United Kingdom.

Despite improvements in assisted reproduction techniques (ARTs), live birth rates remain suboptimal, particularly in women with advanced maternal age (AMA). The leading cause of poor reproductive outcomes demonstrated in women with AMA, as well as women with recurrent miscarriage and repetitive implantation failure, is thought to be due to high rates of embryonic aneuploidy. Preimplantation genetic testing for aneuploidies (PGT-A) aims to select an euploid embryo for transfer and therefore improve ART outcomes. Read More

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Supernumerary embryos, do they show the cycle success in a fresh embryo transfer? A retrospective analysis.

Gynecol Endocrinol 2021 Jul 7:1-4. Epub 2021 Jul 7.

Department of Obstetrics & Gynecology, ART Center, Bursa Uludag University School of Medicine, Gorukle, Turkey.

Objective: To determine the predictive value of cryopreserved embryos during single fresh blastocyst transfer.

Methods: This retrospective cohort study was conducted at a tertiary university hospital ART center. Patients who underwent a single fresh blastocyst transfer were selected from the electronic database. Read More

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Low Molecular Weight Heparins (LMWH) and Implications along Pregnancy: a Focus on the Placenta.

Reprod Sci 2021 Jul 6. Epub 2021 Jul 6.

Department of Experimental and Clinical Biomedical Sciences, Obstetrics and Gynecology, University of Florence, Viale Morgagni 44, 50134, Florence, Italy.

Low molecular weight heparins (LMWH) have been largely studied for their use during pregnancy. The biology and the pharmacology of these molecules are well known and may be summarized in three main mechanisms of action: anti-coagulant, anti-inflammatory, and immunomodulant. The clinical implications of these drugs during pregnancy are mainly related to their action on the placenta, because of the presence of specific molecular and cellular targets, particularly at the trophoblast-endometrial interface. Read More

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Caesarean section defects may affect pregnancy outcomes after in vitro fertilization-embryo transfer: a retrospective study.

BMC Pregnancy Childbirth 2021 Jul 6;21(1):487. Epub 2021 Jul 6.

Center for Reproductive Medicine, Tianjin Central Hospital of Obstetrics and Gynecology, No. 156 Nankai San Ma Road, Tianjin, 300000, China.

Background: Caesarean section rates are rising worldwide. One adverse effect of caesarean section reported in some studies is an increased risk of subfertility. Only a few studies have assessed the relationship between the previous mode of delivery and in vitro fertilization/intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET) reproductive outcomes. Read More

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