1,277 results match your criteria el4 cells

The Effects of Adoptively Transferred IL-23/IL-18-Polarized Neutrophils on Tumor and Collagen-Induced Arthritis in Mice.

J Inflamm Res 2021 16;14:4669-4686. Epub 2021 Sep 16.

Department of State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, People's Republic of China.

Background: Neutrophils present great diverse phenotypes in various microenvironments and play different immune regulatory functions. Neutrophils generally classified into inflammatory phenotype N1 and anti-informatory phenotype N2. Our recent studies showed that IL-23 alone stimulated neutrophils to express IL-17A, IL-17F and IL-22 and displayed a gene transcriptional profile similar to Th17 cells. Read More

View Article and Full-Text PDF
September 2021

Sesamin promotes apoptosis and pyroptosis via autophagy to enhance antitumour effects on murine T-cell lymphoma.

J Pharmacol Sci 2021 Nov 10;147(3):260-270. Epub 2021 Aug 10.

Shenzhen Hospital, Southern Medical University, Shenzhen, Guangdong, 518100, China. Electronic address:

Sesamin is a lignan compound in plants that has various pharmacological effects, including reducing diabetes-associated injuries, regulating fatty acid and cholesterol metabolism, and exerting antiinflammatory and antitumour effects. Previous studies have reported that sesamin can inhibit the proliferation of several types of tumour cells and exert antitumour effects. However, the antitumour effect of sesamin on T-cell lymphoma is still unknown. Read More

View Article and Full-Text PDF
November 2021

The T/Tn-Specific Lectin Induces Cell Death in Lymphoma Cells Negative for T/Tn Antigens.

Cancers (Basel) 2021 Aug 28;13(17). Epub 2021 Aug 28.

UMR 152 PharmaDev, Institut de Recherche et Développement, Faculté de Pharmacie, Université Paul, Sabatier, F-31062 Toulouse, France.

Morniga G is a T/Tn-specific lectin, inducing cell death in Tn-positive leukemias but not in healthy lymphocytes. lectin (HPA) is another T/Tn-specific lectin, currently used as tool for cancer diagnostics. The HPA-mediated tumor cell death was evaluated on human leukemia and mouse lymphoma cells, and compared to the effect of Morniga G. Read More

View Article and Full-Text PDF

Dynamin inhibition causes context-dependent cell death of leukemia and lymphoma cells.

PLoS One 2021 7;16(9):e0256708. Epub 2021 Sep 7.

Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.

Current chemotherapy for treatment of pediatric acute leukemia, although generally successful, is still a matter of concern due to treatment resistance, relapses and life-long side effects for a subset of patients. Inhibition of dynamin, a GTPase involved in clathrin-mediated endocytosis and regulation of the cell cycle, has been proposed as a potential anti-cancer regimen, but the effects of dynamin inhibition on leukemia cells has not been extensively addressed. Here we adopted single cell and whole-population analysis by flow cytometry and live imaging, to assess the effect of dynamin inhibition (Dynasore, Dyngo-4a, MitMAB) on pediatric acute leukemia cell lines (CCRF-CEM and THP-1), human bone marrow biopsies from patients diagnosed with acute lymphoblastic leukemia (ALL), as well as in a model of lymphoma (EL4)-induced tumor growth in mice. Read More

View Article and Full-Text PDF
September 2021

Doxorubicin Paradoxically Ameliorates Tumor-Induced Inflammation in Young Mice.

Int J Mol Sci 2021 Aug 21;22(16). Epub 2021 Aug 21.

Department of Experimental and Clinical Pharmacology, College of Pharmacy, University of Minnesota, Minneapolis, MN 55455, USA.

Doxorubicin (DOX) is one of the most widely used chemo-therapeutic agents in pediatric oncology. DOX elicits an inflammatory response in multiple organs, which contributes to DOX-induced adverse effects. Cancer itself causes inflammation leading to multiple pathologic conditions. Read More

View Article and Full-Text PDF

MicroRNA and circRNA Expression Analysis in a Zbtb1 Gene Knockout Monoclonal EL4 Cell Line.

Front Cell Infect Microbiol 2021 5;11:706919. Epub 2021 Jul 5.

College of Veterinary Medicine, Jilin Agricultural University, Changchun, China.

Zinc finger and BTB domain containing 1(Zbtb1) is a transcriptional suppressor protein, and a member of the mammalian Zbtb gene family. Previous studies have shown that Zbtb1 is essential for T-cell development. However, the role of Zbtb1 in T-cell lymphoma is undetermined. Read More

View Article and Full-Text PDF

Belantamab Mafodotin (GSK2857916) Drives Immunogenic Cell Death and Immune-mediated Antitumor Responses .

Mol Cancer Ther 2021 Jul 12. Epub 2021 Jul 12.

Oncology R&D, GlaxoSmithKline, Collegeville, Pennsylvania.

B-cell maturation antigen (BCMA) is an attractive therapeutic target highly expressed on differentiated plasma cells in multiple myeloma and other B-cell malignancies. GSK2857916 (belantamab mafodotin, BLENREP) is a BCMA-targeting antibody-drug conjugate approved for the treatment of relapsed/refractory multiple myeloma. We report that GSK2857916 induces immunogenic cell death in BCMA-expressing cancer cells and promotes dendritic cell activation and GSK2857916 treatment enhances intratumor immune cell infiltration and activation, delays tumor growth, and promotes durable complete regressions in immune-competent mice bearing EL4 lymphoma tumors expressing human BCMA (EL4-hBCMA). Read More

View Article and Full-Text PDF

Irreversible electroporation enhances immunotherapeutic effect in the off-target tumor in a murine model of orthotopic HCC.

Am J Cancer Res 2021 15;11(6):3304-3319. Epub 2021 Jun 15.

Department of Surgery, School of Medicine, University of Louisville Louisville, KY 40202, USA.

Irreversible electroporation (IRE) has been postulated to have an off-target effect on lesions not in the tumor-ablative field, possibly through heightened immunologic response. In this study, we evaluated whether combination IRE and immunotherapy would lead to increased tumor necrosis and T cell recruitment to both the treated tumors and tumors outside the local ablative field. An cell-IRE model was established to evaluate the ability of T lymphocytes (EL4 cell and HH cells) migration in response to Hepatocellular carcinoma (HCC) cells (Hepa1-6 and HepG2) with IRE treatment. Read More

View Article and Full-Text PDF

Extracellular loops of the serotonin transporter act as a selectivity filter for drug binding.

J Biol Chem 2021 07 9;297(1):100863. Epub 2021 Jun 9.

Institute of Pharmacology and the Gaston H. Glock Research Laboratories for Exploratory Drug Development, Center of Physiology and Pharmacology, Medical University of Vienna, Austria.

The serotonin transporter (SERT) shapes serotonergic neurotransmission by retrieving its eponymous substrate from the synaptic cleft. Ligands that discriminate between SERT and its close relative, the dopamine transporter DAT, differ in their association rate constant rather than their dissociation rate. The structural basis for this phenomenon is not known. Read More

View Article and Full-Text PDF

GM-CSF mediates immune evasion via upregulation of PD-L1 expression in extranodal natural killer/T cell lymphoma.

Mol Cancer 2021 05 29;20(1):80. Epub 2021 May 29.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, 510060, Guangdong, China.

Background: Granulocyte-macrophage colony stimulating factor (GM-CSF) is a cytokine that is used as an immunopotentiator for anti-tumor therapies in recent years. We found that some of the extranodal natural killer/T cell lymphoma (ENKTL) patients with the treatment of hGM-CSF rapidly experienced disease progression, but the underlying mechanisms remain to be elucidated. Here, we aimed to explore the mechanisms of disease progression triggered by GM-CSF in ENKTL. Read More

View Article and Full-Text PDF

Modulation of the anticancer activities of paclitaxel by Cremophor micelles.

Int J Pharm 2021 Jun 14;603:120699. Epub 2021 May 14.

School of Pharmacy, College of Medicine, National Taiwan University, 33 Linsen South Road, Room 423, Taipei City 10050, Taiwan. Electronic address:

The objective of this study was to determine the effect of Cremophor (CrEL) on the antineoplastic effect induced by paclitaxel (PTX). Fluorescence spectroscopy, employing pyrene as a probe, was used to determine the critical micelle concentration (CMC) of CrEL. EL4 murine thymoma cells and MDA-MB-231 human breast cancer cells were treated with PTX in different concentrations of CrEL. Read More

View Article and Full-Text PDF

Hydroxyapatite-Coated SPIONs and Their Influence on Cytokine Release.

Int J Mol Sci 2021 Apr 16;22(8). Epub 2021 Apr 16.

Department of Otorhinolaryngology, Head and Neck Surgery, Section of Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung-Professorship, Universitätsklinikum Erlangen, 91054 Erlangen, Germany.

Hydroxyapatite- or calcium phosphate-coated iron oxide nanoparticles have a high potential for use in many biomedical applications. In this study, a co-precipitation method for the synthesis of hydroxyapatite-coated nanoparticles (SPION), was used. The produced nanoparticles have been characterized by dynamic light scattering, X-ray diffraction, vibrating sample magnetometry, Fourier transform infrared spectrometry, atomic emission spectroscopy, scanning electron microscopy, transmission electron microscopy, selected area diffraction, and energy-dispersive X-ray spectroscopy. Read More

View Article and Full-Text PDF

Monitoring tumor cell death in murine tumor models using deuterium magnetic resonance spectroscopy and spectroscopic imaging.

Proc Natl Acad Sci U S A 2021 03;118(12)

Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge CB2 0RE, United Kingdom;

H magnetic resonance spectroscopic imaging has been shown recently to be a viable technique for metabolic imaging in the clinic. We show here that H MR spectroscopy and spectroscopic imaging measurements of [2,3-H]malate production from [2,3-H]fumarate can be used to detect tumor cell death in vivo via the production of labeled malate. Production of [2,3-H]malate, following injection of [2,3-H]fumarate (1 g/kg) into tumor-bearing mice, was measured in a murine lymphoma (EL4) treated with etoposide, and in human breast (MDA-MB-231) and colorectal (Colo205) xenografts treated with a TRAILR2 agonist, using surface-coil localized H MR spectroscopy at 7 T. Read More

View Article and Full-Text PDF

Antitumor Effect against Murine Cells of CT26 Colon Cancer and EL4 Lymphoma by Autologous Whole Tumor Dead Cells.

Biomed Res Int 2021 3;2021:6626851. Epub 2021 Feb 3.

Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, USACH, Alameda, 3363 Santiago, Chile.

Active immunotherapy against cancer is based on immune system stimulation, triggering efficient and long-lasting antigen-specific immune responses. Immunization strategies using whole dead cells from tumor tissue, containing specific antigens inside, have become a promising approach, providing efficient lymphocyte activation through dendritic cells (DCs). In this work, we generate whole dead tumor cells from CT26, E. Read More

View Article and Full-Text PDF

Self-Cutting and Integrating CRISPR Plasmids Enable Targeted Genomic Integration of Genetic Payloads for Rapid Cell Engineering.

CRISPR J 2021 02;4(1):104-119

Human Health Therapeutics Research Center, National Research Council Canada, Ottawa, Canada.

Since observations that CRISPR nucleases function in mammalian cells, many strategies have been devised to adapt them for genetic engineering. Here, we investigated self-cutting and integrating CRISPR-Cas9 plasmids (SCIPs) as easy-to-use gene editing tools that insert themselves at CRISPR-guided locations. SCIPs demonstrated similar expression kinetics and gene disruption efficiency in mouse (EL4) and human (Jurkat) cells, with stable integration in 3-6% of transfected cells. Read More

View Article and Full-Text PDF
February 2021

Extracts of select endemic plants from the Republic of Mauritius exhibiting anti-cancer and immunomodulatory properties.

Sci Rep 2021 Feb 19;11(1):4272. Epub 2021 Feb 19.

Department of Pathology, Microbiology & Immunology, School of Medicine, University of South Carolina, 202 Osborne Administration Building, Columbia, SC, 29208, USA.

Mauritius Island possesses unique plant biodiversity with a potential reservoir of biologically active compounds of pharmacological interest. In the current study, we investigated Mauritius endemic plant families Asteraceae, Ebenaceae, Sapotaceae, and Erythroxylaceae, for anti-cancer properties on T cell lymphoma and B16F10 Melanoma cells and immunomodulatory properties on primary T and B cells. The cytotoxicity of methanolic plant extracts at 1, 10, 25 µg/ml was determined. Read More

View Article and Full-Text PDF
February 2021

Overexpression of Interleukin-1α Suppresses Liver Metastasis of Lymphoma: Implications for Antitumor Effects of CD8+ T-cells.

J Histochem Cytochem 2021 04 9;69(4):245-255. Epub 2021 Feb 9.

Department of Orthopaedic Surgery, Keio University School of Medicine, Tokyo, Japan.

Interleukin (IL)-1 plays a key role in carcinogenesis, tumor progression, and metastasis. Although IL-1 may enhance the expansion of CD8+ T-cells, the pathological contribution of IL-1-activated CD8+ T-cells to tumor metastasis remains unclear. This study used a liver metastasis model of the EL4 T-cell lymphoma cells transplanted into human IL (hIL)-1α conditional transgenic (hIL-1α cTg) mice. Read More

View Article and Full-Text PDF

Effect of low-dose corticosterone pretreatment on the production of inflammatory mediators in super-low-dose LPS-primed immune cells.

Byeong Suk Chae

Toxicol Res 2021 Jan 12;37(1):47-57. Epub 2020 Aug 12.

College of Pharmacy, Woosuk University, Wanju, Jeonbuk 55338 Republic of Korea.

Pretreatment of super-low-dose lipopolysaccharide (SL-LPS) induces a more hyperresponsive state on the production of proinflammatory mediators to a subsequent secondary challenge with high-dose LPS in innate immune cells. Low-dose glucocorticoids (GCs) are also known to induce inflammation and immunosuppression in the immune cells. However, there is limited knowledge on whether preconditioning of low-dose GCs enhances inflammatory responses and dysregulates T lymphocyte responses to secondary LPS in SL-LPS-primed immune cells. Read More

View Article and Full-Text PDF
January 2021

EL4-derived Exosomes Carry Functional TNF-related Apoptosis-inducing Ligand that are Able to Induce Apoptosis and Necrosis in the Target Cells.

Int J Mol Cell Med 2020 10;9(3):207-215. Epub 2020 Nov 10.

Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Exosomes released by tumor cells play critical roles in tumor progression, immune cell suppression, and cancer metastasis. The aim of the present study was to investigate whether the exosomes released by EL4 cells carry a functional TNF-related apoptosis-inducing ligand (TRAIL) molecule. Exosomes were harvested from the supernatants of EL4 cell culture, and the shape, size, and identity of EL4-derived exosomes were evaluated by utilizing scanning electron microscopy, dynamic light scattering, and dot-blot method. Read More

View Article and Full-Text PDF
November 2020

Improved non-invasive positron emission tomographic imaging of chemotherapy-induced tumor cell death using Zirconium-89-labeled APOMAB®.

EJNMMI Radiopharm Chem 2020 Nov 17;5(1):27. Epub 2020 Nov 17.

Translational Oncology Laboratory, Centre for Cancer Biology, SA Pathology and University of South Australia, Adelaide, SA, 5000, Australia.

Purpose: The chimeric monoclonal antibody (mAb) chDAB4 (APOMAB®) targets the Lupus associated (La)/Sjögren Syndrome-B (SSB) antigen, which is over-expressed in tumors but only becomes available for antibody binding in dead tumor cells. Hence, chDAB4 may be used as a novel theranostic tool to distinguish between responders and nonresponders early after chemotherapy. Here, we aimed to ascertain which positron emitter, Zirconium-89 ([Zr]Zr) or Iodine-124 ([I]I), was best suited to label chDAB4 for post-chemotherapy PET imaging of tumor-bearing mice and to determine which of two different bifunctional chelators provided optimal tumor imaging by PET using [Zr]Zr-labeled chDAB4. Read More

View Article and Full-Text PDF
November 2020

Doxorubicin uptake in ascitic lymphoma model: resistance or curability is governed by tumor cell density and prolonged drug retention.

J Cancer 2020 19;11(22):6497-6506. Epub 2020 Sep 19.

Laboratory of Immunology, National Cancer Institute, Vilnius, Lithuania.

Chemotherapy resistance of malignancies is a universal phenomenon which unfavorably affects therapeutic results. Genetic adaptations as well as epigenetic factors can play an important role in the development of multidrug resistance. Cytotoxic drug content in plasma of cancer patients is known to variate up to one hundred-fold regardless of the same dose injected per m body surface. Read More

View Article and Full-Text PDF
September 2020

CSF-1R inhibition disrupts the dialog between leukaemia cells and macrophages and delays leukaemia progression.

J Cell Mol Med 2020 11 10;24(22):13115-13128. Epub 2020 Oct 10.

Department of Pediatrics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Research in the last few years has revealed that leukaemic cells can remodel the bone marrow niche into a permissive environment favouring leukaemic stem cell expansion. Tumour-associated macrophages (TAMs) are prominent components of the tumour microenvironment and play an important role in the onset and progression of solid tumours. However, little is known about their role in the development of acute lymphoblastic leukaemia (ALL). Read More

View Article and Full-Text PDF
November 2020

Intestinal Nematode Infection Affects Metastasis of EL4 Lymphoma Cells.

Arch Immunol Ther Exp (Warsz) 2020 Sep 22;68(5):30. Epub 2020 Sep 22.

Laboratory of Molecular Oncology and Innovative Therapies, Military Institute of Medicine, Warsaw, Poland.

An effective host immune system prevents the growth of most cancer cells. However, as intestinal nematodes are able to induce both immunotolerance and immunosuppression in the host, it is possible that their presence could allow co-occurring cancer cells to proliferate and metastasize. Our findings indicate that previous, subsequent or concurrent intestinal nematode infection affects the formation of lung metastatic nodules in mice experimentally infected with Heligmosomoides polygyrus. Read More

View Article and Full-Text PDF
September 2020

PD-1 antibody and ruxolitinib enhances graft-versus-lymphoma effect without increasing acute graft-versus-host disease in mice.

Am J Transplant 2021 02 5;21(2):503-514. Epub 2020 Sep 5.

Blood Diseases Institute, Xuzhou Medical University, Xuzhou, China.

Boosting T cell immune response posttransplant with checkpoint inhibitors increases graft-versus-lymphoma (GVL) effects at the cost of increasing acute graft-versus-host disease (aGVHD). A combined targeted therapy is needed to decrease checkpoint inhibitors-induced aGVHD without impairing GVL. We studied whether this competition could be avoided by giving concurrent anti-PD-1 antibody and ruxolitinib in allotransplant mouse models in which recipients were challenged with A20 or EL4 lymphoma cells. Read More

View Article and Full-Text PDF
February 2021

Novel chimerized IgA CD20 antibodies: Improving neutrophil activation against CD20-positive malignancies.

MAbs 2020 Jan-Dec;12(1):1795505

Center for Translational Immunology, University Medical Center Utrecht , Utrecht, The Netherlands.

Current combination therapies elicit high response rates in B cell malignancies, often using CD20 antibodies as the backbone of therapy. However, many patients eventually relapse or develop progressive disease. Therefore, novel CD20 antibodies combining multiple effector mechanisms were generated. Read More

View Article and Full-Text PDF

miR-182-5p inhibits the pathogenic Th17 response in experimental autoimmune uveitis mice via suppressing TAF15.

Biochem Biophys Res Commun 2020 08 20;529(3):784-792. Epub 2020 Jul 20.

Department of Laboratory Medicine, First People's Hospital of Yunnan Province, Kunming, China. Electronic address:

Background: CD4 T helper 17 (Th17) cells play a contributory role in uveitis and other autoimmune disorders. However, less is understood about the contribution of microRNAs (miRNAs) in regulating the pathogenic Th17 response in uveitis.

Methods: The in vivo experimental autoimmune uveitis (EAU) model was constructed in female C57BL/6 mice. Read More

View Article and Full-Text PDF

Bioactive Sterol Derivatives Isolated from the var. Roseus Induced Apoptosis in Cancer Cell Lines.

Cardiovasc Hematol Agents Med Chem 2020 ;18(2):124-134

Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai- 600 025, Tamil Nadu, India

Objective: The aim of the present study is to isolate and characterize the bioactive compounds from Pleurotus djamor against human breast cancer (MDA-MD-231) and mouse T cell lymphoma (EL4) cell lines.

Materials And Methods: Sequential fractionization and column chromatography methods were involved in compound isolation. The structures of the isolated compound were determined by NMR, GC/MS, and X-ray crystallography studies. Read More

View Article and Full-Text PDF

Development of anti-human CADM1 monoclonal antibodies as a potential therapy for adult T-cell leukemia/lymphoma.

Int J Hematol 2020 Oct 12;112(4):496-503. Epub 2020 Jul 12.

Division of Tumor and Cellular Biochemistry, Department of Medical Sciences, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki, 889-1692, Japan.

Adult T-cell leukemia/lymphoma (ATLL) is a highly invasive and refractory T-cell malignancy, with poor prognosis. We previously identified that cell adhesion molecule 1 (CADM1) is overexpressed consistently in ATLL cells, and that CADM1 expression increases the adhesion capacity of ATLL cells to endothelial cells and promotes the organ invasion of ATLL cells in a xenograft mouse model. In this study, we first show that newly developed several anti-human CADM1 antibodies, which were complete human IgG antibodies generated by phage display method, specifically recognize CADM1 on ATLL cells. Read More

View Article and Full-Text PDF
October 2020

Stem cell factor produced by tumor cells expands myeloid-derived suppressor cells in mice.

Sci Rep 2020 07 9;10(1):11257. Epub 2020 Jul 9.

Division of Liver and Transplantation Surgery, Department of General Surgery, Chang-Gung Memorial Hospital, Chang-Gung University College of Medicine, 5, Fu-Hsing Street, Kwei-Shan Township, Taoyuan, Taiwan.

Immunotherapy is a novel treatment approach for cancers; however, its therapeutic effects are impeded by myeloid-derived suppressor cells (MDSCs). This study aimed to determine how MDSCs are expanded in cancer hosts. MDSCs were positive for Gr-1 and CD11b. Read More

View Article and Full-Text PDF