164 results match your criteria ehdv serotype


A Duplex Fluorescent Microsphere Immunoassay for Detection of Bluetongue and Epizootic Hemorrhagic Disease Virus Antibodies in Cattle Sera.

Viruses 2021 04 15;13(4). Epub 2021 Apr 15.

Center for Grain and Animal Health, Agricultural Research Service, Arthropod-Borne Animal Diseases Research Unit, United States Department of Agriculture, Manhattan, KS 66502, USA.

Bluetongue virus (BTV) causes internationally reportable hemorrhagic disease in cattle, sheep, and white-tailed deer. The closely related, and often co-circulating, epizootic hemorrhagic disease virus causes a clinically similar devastating disease in white-tailed deer, with increasing levels of disease in cattle in the past 10 years. Transmitted by biting midges, together, they constitute constant disease threats to the livelihood of livestock owners. Read More

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Vector Competence of Florida (Diptera: Ceratopogonidae) for Epizootic Hemorrhagic Disease Virus Serotype-2.

Viruses 2021 03 5;13(3). Epub 2021 Mar 5.

Florida Medical Entomology Laboratory, Institute of Food and Agricultural Sciences, University of Florida, Vero Beach, FL 32962, USA.

Epizootic hemorrhagic disease virus (EHDV; family , genus ) is an arthropod-borne virus of ungulates, primarily white-tailed deer in North America. , the only confirmed North American vector of EHDV, is rarely collected from Florida despite annual virus outbreaks. is an abundant species in Florida and is also a confirmed vector of the closely related Bluetongue virus. Read More

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The Effect of Maternal Antibodies on Clinical Response to Infection with Epizootic Hemorrhagic Disease Virus in White-Tailed Deer (Odocoileus virginianus) Fawns.

J Wildl Dis 2021 01;57(1):189-193

Southeastern Cooperative Wildlife Disease Study, Department of Population Health, College of Veterinary Medicine, University of Georgia, 589 D. W. Brooks Drive, Athens, Georgia 30602, USA.

We investigated whether naturally acquired maternal antibodies to epizootic hemorrhagic disease virus serotype 2 (EHDV-2) would protect white-tailed deer (Odocoileus virginianus) fawns against infection and clinical disease following an EHDV-2 challenge. We compared viremia and clinical response in 27-47-d-old, experimentally infected fawns with and without maternally derived antibodies to EHDV-2. Mild to moderate clinical signs were observed in four seronegative (maternal antibody-negative) fawns, which were viremic from 3 to 14 d postinoculation. Read More

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January 2021

Novel Serotype of Epizootic Hemorrhagic Disease Virus, China.

Emerg Infect Dis 2020 12;26(12):3081-3083

In 2018, a strain of epizootic hemorrhagic disease virus (EHDV), named YNDH/V079/2018, was isolated from a sentinel calf in Mangshi County, Yunnan Province, China. Nucleotide sequencing and neutralization tests indicated that the virus belongs to a novel serotype of EHDV that had not been reported previously. Read More

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December 2020

Serologic Evidence of Arthropod-Borne Virus Infections in Wild and Captive Ruminants in Ontario, Canada.

Am J Trop Med Hyg 2020 11;103(5):2100-2107

Southeastern Cooperative Wildlife Disease Study, University of Georgia, Athens, Georgia.

Arthropod-borne viruses (arboviruses) are globally widespread, and their transmission cycles typically involve numerous vertebrate species. Serologic testing of animal hosts can provide a routine surveillance approach to monitoring animal disease systems, can provide a surveillance alternative to arthropod testing and human case reports, and may augment knowledge of epizootiology. Wild and captive ruminants represent good candidate sentinels to track geographic distribution and prevalence of select arboviruses. Read More

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November 2020

An Embryonated Egg Transmission Model for Epizootic Hemorrhagic Disease Virus.

Vector Borne Zoonotic Dis 2020 09 18;20(9):728-730. Epub 2020 May 18.

Southeastern Cooperative Wildlife Disease Study, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA.

Epizootic hemorrhagic disease virus (EHDV) is a vector-borne orbivirus of ruminants; in North America there are three serotypes (EHDV-1, -2, and -6) and these primarily affect white-tailed deer (). EHDV is vectored by biting midges, spp. Embryonated chicken eggs (ECE) have recently been used as an experimental host to investigate the vector competence of Australian spp. Read More

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September 2020

Prospective Study of Epizootic Hemorrhagic Disease Virus and Bluetongue Virus Transmission in Captive Ruminants.

J Med Entomol 2020 07;57(4):1277-1285

Department of Entomology, Louisiana State University Agricultural Center, Agricultural Experiment Station, Life Sciences, Baton Rouge, LA.

Bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) cause hemorrhagic disease (HD) in wild ruminants and bluetongue disease (BT) and epizootic hemorrhagic disease (EHD) in livestock. These viruses are transmitted by biting midges in the genus Culicoides (family Ceratopogonidae). Mortality from this disease can reach 90% in certain breeds of sheep and in white-tailed deer (Odocoileus virginianus). Read More

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Experimental infection of calves with seven serotypes of Epizootic Hemorrhagic Disease virus: production and characterization of reference sera.

Vet Ital 2019 Dec 31;55(4):339-346. Epub 2019 Dec 31.

UMR Virologie, INRA, Ecole Nationale Vétérinaire d'Alfort, laboratoire de santé animale d'Alfort, ANSES, Université Paris-Est, 94700 Maisons-Alfort, France.

The aim of this study was to produce reference sera against the seven serotypes of Epizootic hemorrhagic disease virus (EHDV‑1, EHDV‑2, EHDV‑4, EHDV‑5, EHDV‑6, EHDV‑7, and EHDV‑8). In a high containment unit, seven Prim 'Holstein calves were inoculated at day 0 (D0) with the selected strains (1 EHDV serotype per calf ). Blood samples (EDTA and whole blood) were periodically taken from D0 until the end of the experiment (D31). Read More

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December 2019

Identification and complete-genome phylogenetic analysis of an epizootic hemorrhagic disease virus serotype 7 strain isolated in China.

Arch Virol 2019 Dec 19;164(12):3121-3126. Epub 2019 Sep 19.

Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, Heilongjiang, People's Republic of China.

An epizootic hemorrhagic disease virus (EHDV) strain designated YN09-04 was isolated from sentinel cattle in China. The length of its complete genome was 19,344 bp in total, consisting of 10 segments ranging in size from 810 bp (S10) to 3942 bp (S1). Based on phylogenetic analysis of the S2 sequence, YN09-04 clusters with EHDV serotype 7 (EHDV-7) strains form a distinct, well-supported subgroup, indicating that YN09-04 belongs to EHDV-7. Read More

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December 2019

Antibodies to Epizootic Hemorrhagic Disease Virus (EHDV) in Farmed and Wild Florida White-Tailed Deer ().

J Wildl Dis 2020 01 12;56(1):208-213. Epub 2019 Jul 12.

Department of Wildlife Ecology and Conservation, 110 Newins-Ziegler Hall, University of Florida, Gainesville, Florida 32611, USA.

The transmission of multiple serotypes of epizootic hemorrhagic disease virus (EHDV) between farmed and free-ranging wildlife is of interest to livestock industries and natural resource agencies. We compared the seroprevalence of EHDV-1, -2, and -6 in wild and farmed white-tailed deer () herds in Florida, US. We compared serological prevalence, circulating serotypes, antibody titers, and viremia with the use of 171 whole-blood samples from 150 unvaccinated white-tailed deer from farm pens, a farm preserve, and wild deer on adjacent public lands between March 2016 and May 2017. Read More

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January 2020

Detection of a novel reassortant epizootic hemorrhagic disease virus serotype 6 in cattle in Trinidad, West Indies, containing nine RNA segments derived from exotic EHDV strains with an Australian origin.

Infect Genet Evol 2019 10 22;74:103931. Epub 2019 Jun 22.

Non-vesicular reference laboratory, The Pirbright Institute, Woking, Surrey GU24 0NF, UK.

Epizootic hemorrhagic disease virus (EHDV) is a Culicoides-transmitted orbivirus that infects domestic and wild ruminants in many parts of the world. Of the eight proposed serotypes, only EHDV-1, 2 and 6 have been reported to be present in the Americas. Following the identification of a virulent EHD-6 reasssortant virus in the USA in 2007 (EHDV-6 Indiana), with outer coat protein segments derived from an Australian strain of EHDV and all remaining segments derived from a locally circulating EHDV-2 strain, questions have remained about the origin of the Australian parent strain and how it may have arrived in the USA. Read More

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October 2019

Inter-serotype reassortment among epizootic haemorrhagic disease viruses in the United States.

Transbound Emerg Dis 2019 Sep 12;66(5):1809-1820. Epub 2019 Jun 12.

Department of Veterinary Clinical Medicine and the Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Illinois, Urbana, Illinois.

First described in 1955 in New Jersey, epizootic haemorrhagic disease (EHD) causes a severe clinical disease in wild and domestic ruminants worldwide. Epizootic haemorrhagic disease outbreaks occur in deer populations each year from summer to late autumn. The etiological agent is EHD virus (EHDV) which is a double-stranded segmented icosahedral RNA virus. Read More

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September 2019

Field data implicating Culicoides stellifer and Culicoides venustus (Diptera: Ceratopogonidae) as vectors of epizootic hemorrhagic disease virus.

Parasit Vectors 2019 May 23;12(1):258. Epub 2019 May 23.

Florida Medical Entomology Laboratory, University of Florida, Vero Beach, FL, USA.

Background: Epizootic hemorrhagic disease virus (EHDV) is an Orbivirus of veterinary importance which is transmitted by biting midges of the genus Culicoides (Diptera: Ceratopogonidae) to ruminants. Culicoides sonorensis Wirth & Jones, the only confirmed vector of EHDV in the USA, is rare in the southeastern states where transmission persists, suggesting that other Culicoides species transmit EHDV in this region. The present study aimed to determine which Culicoides species transmitted EHDV in Florida and Alabama, two states in the southeastern USA. Read More

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Production of monoclonal antibodies binding to the VP7 protein of African horse sickness virus.

J Virol Methods 2019 08 13;270:131-136. Epub 2019 May 13.

Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale", Teramo, Italy.

Monoclonal antibodies (MAbs) against AHSV were produced by immunising BALB/c mice with AHSV serotype 9 and six clones able to recognize specifically the VP7-AHSV with a strong reactivity were selected. The specificity of the MAbs was assessed in i-ELISA against a commercial VP7-AHSV and in immunoblot against a home-made VP7-AHSV, expressed by a Baculovirus expression system; potential cross-reactions with related orbiviruses (Bluetongue virus and Epizootic Haemorrhagic Disease virus) were investigated as well. One of the six MAbs selected, MAb 7F11E14, was tested in direct immunofluorescence and reacted with all nine AHSV serotypes, but didn't cross-react with BTV and EHDV. Read More

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Presence of bluetongue and epizootic hemorrhagic disease viruses in Egypt in 2016 and 2017.

Infect Genet Evol 2019 09 30;73:221-226. Epub 2019 Apr 30.

Université Paris Est, ANSES, ENVA, INRA, UMR 1161 VIROLOGIE, Laboratory for Animal Health, Maisons-Alfort, France. Electronic address:

BTV and EHDV are closely-related orbiviruses that are transmitted between domestic and wild ruminants via the bites of hematophagous midges. Previous studies have reported seropositivity against BTV antibodies in sheep and goats in two Egyptian governorates (Beni Suef and Menoufia). However, no recent data are available on the BTV serotype(s) circulating in Egypt and the likely presence of EHDV has never been explored. Read More

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September 2019

EHDV-2 Infection Prevalence Varies in after Feeding on Infected White-Tailed Deer over the Course of Viremia.

Viruses 2019 04 23;11(4). Epub 2019 Apr 23.

Southeastern Cooperative Wildlife Disease Study, Department of Population Health, College of Veterinary Medicine, University of Georgia, 589 D.W. Brooks Drive, Athens, GA 30602, USA.

Epizootic hemorrhagic disease viruses (EHDVs) are arboviral pathogens of white-tailed deer and other wild and domestic ruminants in North America. Transmitted by various species of , EHDVs circulate wherever competent vectors and susceptible ruminant host populations co-exist. The impact of variation in the level and duration of EHDV viremia in white-tailed deer () on infection prevalence is not well characterized. Read More

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Vector Competence of (Diptera: Ceratopogonidae) for Epizootic Hemorrhagic Disease Virus Serotype 2 Strains from Canada and Florida.

Viruses 2019 04 22;11(4). Epub 2019 Apr 22.

Florida Medical Entomology Laboratory, University of Florida, 200 9th St. SE, Vero Beach, FL 32962, USA.

Epizootic hemorrhagic disease virus (EHDV), an transmitted by spp. vectors, is represented by seven serotypes and numerous strains worldwide. While studies comparing vector competence between serotypes exist, studies between viral strains are lacking. Read More

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Detection of Epizootic Hemorrhagic Disease Virus Serotype 1, Israel.

Emerg Infect Dis 2019 04;25(4):825-827

During September 2016-February 2017, we detected epizootic hemorrhagic disease virus (EHDV) in ruminants in Israel. BLAST and phylogenetic analyses of segment 2 in 6 EHDVs isolated from field samples indicated a close relationship to the EHDV serotype 1 strain in Nigeria. Affected cattle had mostly mild or asymptomatic disease. Read More

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Evaluation of 2012 US EHDV-2 outbreak isolates for genetic determinants of cattle infection.

J Gen Virol 2019 04 14;100(4):556-567. Epub 2019 Mar 14.

1​United States Department of Agriculture, Agricultural Research Service, Arthropod-borne Animal Diseases Research Unit, 1515 College Avenue, Manhattan, KS 66502, USA.

Following a summer of severe drought and abnormally high temperatures, a major outbreak of EHDV occurred during 2012 in the USA. Although EHDV-1, -2 and -6 were isolated, EHDV-2 was the predominant virus serotype detected during the outbreak. In addition to large losses of white-tailed deer, the Midwest and northern Plains saw a significant amount of clinical disease in cattle. Read More

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Identification and characterization of epizootic hemorrhagic disease virus serotype 6 in cattle co-infected with bluetongue virus in Trinidad, West Indies.

Vet Microbiol 2019 Feb 11;229:1-6. Epub 2018 Dec 11.

School of Veterinary Medicine, Faculty of Medical Sciences, The University of theWest Indies, St. Augustine, Trinidad, West Indies.

Epizootic hemorrhagic disease virus (EHDV) is an economically important virus that can cause severe clinical disease in deer and to a lesser extent cattle. This study set out to determine and characterize which EHDV serotypes were circulating in Trinidad. Serum and whole blood samples were collected monthly for six months from a cohort of cattle imported to Trinidad from the USA. Read More

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February 2019

Evidence of bluetongue and Epizootic Haemorrhagic disease circulation on the island of Mayotte.

Acta Trop 2019 Mar 24;191:24-28. Epub 2018 Dec 24.

ASTRE, Univ Montpellier, CIRAD, INRA, Montpellier, France; CIRAD, UMR ASTRE, Sainte Clotilde, La Réunion, France.

A cross-sectional study was conducted to explore the epidemiological situation in Mayotte regarding two orbiviruses: Bluetongue virus (BTV) and Epizootic Haemorrhagic Disease virus (EHDV). In all, 385 individual asymptomatic cattle were blood-sampled (one EDTA and one serum tube per animal) between February and June 2016. Antibody (ELISA) and genome prevalence (PCR) was assessed. Read More

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Evaluation of an IGM-specific ELISA for early detection of bluetongue virus infections in domestic ruminants sera.

Transbound Emerg Dis 2019 Jan 23;66(1):537-545. Epub 2018 Nov 23.

Laboratoire de Santé Animale d'Alfort, Université Paris Est, ANSES, ENVA, INRA, UMR 1161 VIROLOGIE, Maisons-Alfort, France.

Competitive-ELISA (c-ELISA) is the most widely used serological test for the detection of Bluetongue virus (BTV) viral protein 7 (VP7) antibodies (Ab). However, these BTV c-ELISAs cannot to distinguish between IgG and IgM. IgM Ab are generated shortly after the primary immune response against an infectious agent, indicating a recent infection or exposure to antigens, such as after vaccination. Read More

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January 2019

GENETIC RELATEDNESS OF EPIZOOTIC HEMORRHAGIC DISEASE VIRUS SEROTYPE 2 FROM 2012 OUTBREAK IN THE USA.

J Wildl Dis 2019 04 4;55(2):363-374. Epub 2018 Oct 4.

1 Southeastern Cooperative Wildlife Disease Study, Department of Population Health, College of Veterinary Medicine, University of Georgia, 589 D. W. Brooks Dr., Athens, Georgia 30602, USA.

During summer and early fall of 2012, the US experienced the largest outbreak of hemorrhagic disease (HD) on record; deer (both Odocoileus virginianus and Odocoileus hemionus) in 35 states were affected, including many northern states where HD typically does not occur. Epizootic hemorrhagic disease virus (EHDV) was the predominant virus isolated, with serotype 2 (EHDV-2) representing 66% (135/205) of all isolated viruses. Viruses within the EHDV serogroup are genetically similar, but we hypothesized that subtle genetic distinctions between viruses would exist across the geographic range of the outbreak if multiple EHDV-2 strains were responsible. Read More

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Epizootic haemorrhagic disease virus circulation in Tunisia.

Vet Ital 2018 Mar;54(1):87-90

RVT, 20 rue de JebelLakdhar, 1006 Tunis-La Rabta, under the tutelage of Tunis - El Manar University, Tunisia.

Epizootic haemorrhagic disease virus (EHDV) was detected for the first time in Tunisia and in other Northern African countries in 2006. The objective of the present study was to investigate whether EHDV circulated in Tunisian livestock before and after the officially-reported outbreak of 2006. Thus, serum samples from cattle and dromedaries collected in different time periods (before and after 2006) and from different regions of Tunisia were screened for the presence of EHDV antibodies. Read More

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Complete Genome Sequence of Serotype 6, Isolated from Florida White-Tailed Deer (Odocoileus virginianus).

Genome Announc 2018 Apr 5;6(14). Epub 2018 Apr 5.

Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA

Here, we report the complete genome sequence of (EHDV) serotype 6 (EHDV-6), isolated from a Florida white-tailed deer () in 2016. To our knowledge, this is the first full genome sequence determined for an EHDV-6 isolate from Florida. Read More

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A serosurvey of bluetongue and epizootic haemorrhagic disease in a convenience sample of sheep and cattle herds in Zimbabwe.

Onderstepoort J Vet Res 2017 Nov 14;84(1):e1-e5. Epub 2017 Nov 14.

Institute of Veterinary, Animal and Biomedical Sciences, Massey University.

A convenience sample of sheep and cattle herds around the cities of Harare, Kwekwe and Bulawayo, located in the Highveld region of Zimbabwe, was used to estimate the seroprevalence and sero-incidence of bluetongue virus (BTV) and epizootic haemorrhagic disease virus (EHDV) antibodies. A competitive enzyme-linked immunosorbent assay was used to identify serum antibodies against BTV and EHDV across three rainy seasons. The median sero-prevalence of BTV and EHDV antibodies in cattle was 62% (interquartile range [IQR]: 30-89) and 56% (IQR: 5-77), respectively. Read More

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November 2017

Epizootic hemorrhagic disease virus serotype 6 outbreak in Israeli cattle in 2015.

J Vet Diagn Invest 2017 Nov 14;29(6):885-888. Epub 2017 Aug 14.

Division of Virology, Kimron Veterinary Institute, Bet Dagan, Israel (Golender, Khinich, Gorohov, Bumbarov).

In September 2015, a large outbreak caused by epizootic hemorrhagic disease virus (EHDV) was identified in Israeli dairy and beef farms. The main clinical signs were reduced milk production, weakness, drooling, lameness and recumbency, fever, slight erythema of nasal and oral mucosae, weight loss, and abortion. Dyspnea, cachexia, and death were observed less frequently. Read More

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November 2017

The First 10 Years (2006-15) of Epizootic Hemorrhagic Disease Virus Serotype 6 in the USA.

J Wildl Dis 2017 10 28;53(4):901-905. Epub 2017 Jun 28.

1 Southeastern Cooperative Wildlife Disease Study, Department of Population Health, College of Veterinary Medicine, University of Georgia, 589 D. W. Brooks Dr., Athens, Georgia 30602, USA.

Epizootic hemorrhagic disease virus (EHDV) is a Culicoides biting midge-transmitted orbivirus (family Reoviridae) of wild and domestic ruminants and is an important pathogen of white-tailed deer (Odocoileus virginianus). Historically, only two serotypes, EHDV-1 and EHDV-2, have been known to be endemic in the US. However, in 2006, an exotic serotype (EHDV-6) was first detected in the US by a long-term passive surveillance system for EHDV and bluetongue viruses. Read More

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October 2017

Characterization of genome segments 2, 3 and 6 of epizootic hemorrhagic disease virus strains isolated in Japan in 1985-2013: Identification of their serotypes and geographical genetic types.

Infect Genet Evol 2017 09 12;53:38-46. Epub 2017 May 12.

Kyushu Research Station, National Institute of Animal Health (NIAH), National Agriculture and Food Research Organization (NARO), Japan.

We characterized genome segments 2, 3 and 6 (Seg-2, Seg-3 and Seg-6) of 11 Japanese strains of epizootic hemorrhagic disease (EHD) virus (EHDV) isolated in 1985-2013. The Japanese strains were divisible into two groups based on phylogenetic analyses of the nucleotide sequences of Seg-2 and Seg-6. In both of the phylogenetic trees based on Seg-2 and Seg-6, seven of the 11 Japanese strains were grouped together with EHDV-2 and EHDV-7 strains, and the other four Japanese strains were grouped with EHDV-1 strains. Read More

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September 2017

Emerging vector-borne diseases in dromedaries in Tunisia: West Nile, bluetongue, epizootic haemorrhagic disease and Rift Valley fever.

Onderstepoort J Vet Res 2017 Mar 31;84(1):e1-e3. Epub 2017 Mar 31.

Université Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie.

A total of 118 sera were collected during 2016 from two groups of dromedaries from Kebili and Medenine governorates in the south of Tunisia. The aim of this study was to provide the first serological investigation of four emerging vector-borne diseases in two groups of dromedaries in Tunisia. Sera were tested by ELISA and serum neutralisation test to identify West Nile virus (WNV), bluetongue virus (BTV), epizootic haemorrhagic disease virus (EHDV) and Rift Valley fever virus (RVFV). Read More

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