139 results match your criteria egg-specific protein


Three-dimensional structures of avian beta-microseminoproteins: insight from the chicken egg-specific beta-microseminoprotein 3 paralog.

FEBS Open Bio 2021 May 1. Epub 2021 May 1.

INRAE, Université de Tours, BOA, Nouzilly, France.

Beta-microseminoproteins (MSMBs) are small disulfide-rich proteins that are conserved among vertebrates. These proteins exhibit diverse biological activities and were mainly reported to play a role in male fertility, immunity, and embryogenesis. In this work, we focused on the chicken MSMB3 protein that was previously depicted as an egg antibacterial protein. Read More

View Article and Full-Text PDF

Disruption of egg-specific protein causes female sterility in Bombyx mori.

Insect Sci 2021 Feb 24. Epub 2021 Feb 24.

Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China.

Yolk proteins are the main source of nutrients during embryonic and early larval development in oviparous animals. Therefore, vitellogenesis is crucial for reproduction. The silkworm, Bombyx mori, is a model lepidopteran insect in which there are three yolk proteins: vitellin, 30-kDa protein, and egg-specific protein (Esp). Read More

View Article and Full-Text PDF
February 2021

Contrasting the effects of phytase and pure myo-inositol on the performance, digestibility, blood and egg yolk inositol levels and digestion physiology of laying hens.

Br Poult Sci 2021 Mar 10:1-11. Epub 2021 Mar 10.

Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, Canada.

ABSRACT1. An experiment was designed to compare the effects of supplementing laying hen diets with phytase and myo-inositol (inositol).2. Read More

View Article and Full-Text PDF

Use of a gene encoding zona pellucida 4 as a female-specific marker for early stage sexual differentiation and size dimorphism in the pacific abalone Haliotis discus hannai.

Anim Reprod Sci 2021 Feb 8;225:106687. Epub 2021 Jan 8.

Department of Fisheries Biology, PuKyong National University, Busan, 48513, Republic of Korea. Electronic address:

Growth rates of Pacific abalone Haliotis discus hannai are an important trait affecting the economic value in the abalone aquaculture industry. A reverse-transcription polymerase chain reaction (RT-PCR) analyses of tissues from H. discus hannai was conducted for sexually mature gonads to determine male- and female-specific target gene expression, including genes encoding zona pellucida domain 4 (zp4), sperm protein (sp) and lysin (lys), respectively. Read More

View Article and Full-Text PDF
February 2021

Improvement of eggshell quality by dietary N-carbamylglutamate supplementation in laying chickens.

Poult Sci 2020 Aug 24;99(8):4085-4095. Epub 2020 Apr 24.

Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310058, P.R. China. Electronic address:

Egg quality defects seriously reduce the quality grade and increase egg breakage in egg marketing activities. In this study, the effect of N-carbamylglutamate (NCG) on eggshell quality was investigated by evaluating calcium absorption and calcification in laying hens. A total of 30 newly hatched female Hy-Line chicks were randomly assigned to the control group (basal diet) and treatment group (basal diet supplemented with 1% NCG). Read More

View Article and Full-Text PDF

Structure, function, and evolution of -AvBD11, the archetype of the structural avian-double-β-defensin family.

Proc Natl Acad Sci U S A 2020 01 23;117(1):337-345. Epub 2019 Dec 23.

Centre de Biophysique Moléculaire, CNRS, 45071 Orléans, France;

Out of the 14 avian β-defensins identified in the genome, only 3 are present in the chicken egg, including the egg-specific avian β-defensin 11 (-AvBD11). Given its specific localization and its established antibacterial activity, -AvBD11 appears to play a protective role in embryonic development. -AvBD11 is an atypical double-sized defensin, predicted to possess 2 motifs related to β-defensins and 6 disulfide bridges. Read More

View Article and Full-Text PDF
January 2020

Nonspecific expression of fertilization genes in the crown-of-thorns Acanthaster cf. solaris: Unexpected evidence of hermaphroditism in a coral reef predator.

Mol Ecol 2020 01 3;29(2):363-379. Epub 2020 Jan 3.

Department of Biological Sciences, Simon Fraser University, Burnaby, BC, Canada.

The characterization of gene expression in gametes has advanced our understanding of the molecular basis for ecological variation in reproductive success and the evolution of reproductive isolation. These advances are especially significant for ecologically important keystone predators such as the coral-eating crown-of-thorns sea stars (COTS, Acanthaster) which are the most influential predator species in Indo-Pacific coral reef ecosystems and the focus of intensive management efforts. We used RNA-seq and transcriptome assemblies to characterize the expression of genes in mature COTS gonads. Read More

View Article and Full-Text PDF
January 2020

The Natural Course of Immediate-Type Cow's Milk and Egg Allergies in Children.

Int Arch Allergy Immunol 2020 21;181(2):103-110. Epub 2019 Nov 21.

Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea,

Background: Most of children with cow's milk (CM) and hen's egg allergies are known to outgrow their diseases with time, but recent studies have demonstrated that children tend to continue to have allergic symptoms in adolescence and adulthood. The aim of this study was to investigate the natural course of CM and egg allergies in Korean children and analyze prognostic factors.

Methods: In this retrospective study, we reviewed data of children with CM allergy and with egg allergy using medical records and parental telephone interviews. Read More

View Article and Full-Text PDF
February 2020

A Gut Analysis Technique for Pinpointing Egg-Specific Predation Events.

J Insect Sci 2019 May;19(3)

Cooperative Extension and Department of Entomology, University of Arizona, Phoenix, AZ.

A universal food immunomarking technique (UFIT) is described for postmortem gut analysis detection of predation on the egg stage of Lygus hesperus Knight (Hemiptera: Miridae). Collops vittatus Say (Coleoptera: Melyridae) and Hippodamia convergens Guérin-Méneville (Coleoptera: Coccinellidae) were fed a single L. hesperus egg that was marked with rabbit and chicken sera proteins. Read More

View Article and Full-Text PDF

Nuclear import of Xenopus egg extract components into cultured cells for reprogramming purposes: a case study on goldfish fin cells.

Sci Rep 2019 02 27;9(1):2861. Epub 2019 Feb 27.

INRA, UR1037 LPGP, Fish Physiology and Genomics, Campus de Beaulieu, F-35000, Rennes, France.

Reprogramming of cultured cells using Xenopus egg extract involves controlling four major steps: plasma membrane permeabilization, egg factors import into the nucleus, membrane resealing, and cell proliferation. Using propidium iodide to assess plasma membrane permeability, we established that 90% of the cultured fin cells were permeabilized by digitonin without any cell losses. We showed that egg extract at metaphase II stage was essential to maintain nuclear import function in the permeabilized cells, as assessed with a fusion GFP protein carrying the nuclear import signal NLS. Read More

View Article and Full-Text PDF
February 2019

Palmar hyperlinearity in early childhood atopic dermatitis is associated with filaggrin mutation and sensitization to egg.

Pediatr Dermatol 2019 Mar 27;36(2):213-218. Epub 2019 Feb 27.

Department of Dermatology, Hamamatsu University School of Medicine, Hamamatsu, Japan.

Background/objectives: Palmar hyperlinearity is a typical clinical feature of Filaggrin gene (FLG) null mutations. There are reports of FLG mutations and allergic sensitization; however, reports on the relationship between palmar hyperlinearity to sensitization are limited. This study aimed to examine the association between palmar hyperlinearity and sensitization in atopic dermatitis (AD) children. Read More

View Article and Full-Text PDF

Isolated Schistosoma mansoni eggs prevent allergic airway inflammation.

Parasite Immunol 2018 Oct 16;40(10):e12579. Epub 2018 Sep 16.

Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands.

Chronic helminth infection with Schistosoma (S.) mansoni protects against allergic airway inflammation (AAI) in mice and is associated with reduced Th2 responses to inhaled allergens in humans, despite the presence of schistosome-specific Th2 immunity. Schistosome eggs strongly induce type 2 immunity and allow to study the dynamics of Th2 versus regulatory responses in the absence of worms. Read More

View Article and Full-Text PDF
October 2018

Egg-specific IgE and basophil activation but not egg-specific T-cell counts correlate with phenotypes of clinical egg allergy.

J Allergy Clin Immunol 2018 07 5;142(1):149-158.e8. Epub 2018 Mar 5.

Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, NY.

Background: Egg allergy is phenotypically heterogeneous. A subset of patients with egg allergy can tolerate egg in an extensively heated form. Inclusion of baked egg (BE) into the diet accelerates resolution of egg allergy. Read More

View Article and Full-Text PDF

The Unique Features of Proteins Depicting the Chicken Amniotic Fluid.

Mol Cell Proteomics 2019 03 14;18(Suppl 1):S174-S190. Epub 2018 Feb 14.

BOA, INRA, Université de Tours, 37380 Nouzilly, France;. Electronic address:

In many amniotes, the amniotic fluid is depicted as a dynamic milieu that participates in the protection of the embryo (cushioning, hydration, and immunity). However, in birds, the protein profile of the amniotic fluid remains unexplored, even though its proteomic signature is predicted to differ compared with that of humans. In fact, unlike humans, chicken amniotic fluid does not collect excretory products and its protein composition strikingly changes at mid-development because of the massive inflow of egg white proteins, which are thereafter swallowed by the embryo to support its growth. Read More

View Article and Full-Text PDF

Early-life gut microbiome and egg allergy.

Allergy 2018 Jul 15;73(7):1515-1524. Epub 2018 Mar 15.

Department of Genetics and Genomic Sciences, Icahn Institute for Genomics and Multiscale Biology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

Background: Gut microbiota may play a role in egg allergy. We sought to examine the association between early-life gut microbiota and egg allergy.

Methods: We studied 141 children with egg allergy and controls from the multicenter Consortium of Food Allergy Research study. Read More

View Article and Full-Text PDF

Vaccine to fibroblast growth factor 23 peptides increases eggshell strength.

Poult Sci 2018 Mar;97(3):882-889

Department of Animal Sciences, 1675 Observatory Drive, University of Wisconsin-Madison, 53706.

Strategies that would increase eggshell quality could be of considerable value to egg producers. This research demonstrated the effective use of fibroblast growth factor 23 (FGF-23) peptide vaccines to increase eggshell quality of Single Comb White Leghorn laying hens (from 69 to 72 wk of age). Hens, fed a standard diet (containing 900 IU/kg vitamin D3), were intramuscularly injected (and boosted) with either a control vaccine (n = 14 hens) or one of 2 FGF-23 peptide vaccines (peptides NP1, GMNPPPYS; and NP7, YTSTERNSFH; n = 15 hens for each peptide). Read More

View Article and Full-Text PDF

Alterations in apoptotic markers and egg-specific protein gene expression in the chicken oviduct during pause in laying induced by tamoxifen.

Theriogenology 2018 Jan 21;105:126-134. Epub 2017 Sep 21.

Department of Animal Physiology and Endocrinology, University of Agriculture in Krakow, Al. Mickiewicza 24/28, 30-059 Krakow, Poland. Electronic address:

The aim of this study was to examine the cell apoptosis, gene expression and activity of caspases 2, 3, 8 and 9, and the mRNA expression of selected egg-specific proteins in the chicken oviduct during pause in egg laying induced by tamoxifen (TMX) treatment. The experiment was carried out on Hy-Line Brown laying hens. The control birds were treated subcutaneously with vehicle (ethanol) and the experimental ones with TMX at a dose of 6 mg/kg of body weight. Read More

View Article and Full-Text PDF
January 2018

Randomised controlled trial of a baked egg intervention in young children allergic to raw egg but not baked egg.

World Allergy Organ J 2017 16;10(1):22. Epub 2017 Jun 16.

Children's Nutrition Research Centre, South Australian Health Medical Research Institute, 72 King William Road, North Adelaide, South Australia 5006 Australia.

Background: Consumption of baked egg by raw egg allergic children is associated with immune changes suggesting development of tolerance. However, causation has not been tested using a double blind randomized controlled trial (RCT). We aimed to compare clinical and immunological outcomes after baked egg (BE) consumption in young BE tolerant egg allergic children. Read More

View Article and Full-Text PDF

Effect of growth hormone on steroid concentrations and mRNA expression of their receptor, and selected egg-specific protein genes in the chicken oviduct during pause in laying induced by fasting.

Domest Anim Endocrinol 2017 10 8;61:1-10. Epub 2017 May 8.

Department of Animal Physiology and Endocrinology, University of Agriculture in Krakow, Krakow, Poland. Electronic address:

This study was undertaken to examine the effect of growth hormone (GH) treatment during pause in laying on (1) the concentration of steroids in blood plasma and oviduct tissues, (2) the expression of mRNA of steroid receptors, and (3) the mRNA expression of selected egg-specific proteins in the chicken oviduct. A pause in egg laying was induced by food deprivation for 5 d, followed by feeding every other day, and then feeding daily from Day 10 onward. Birds were divided into three groups: control (n = 18) fed ad libitum, subjected to pause in laying (n = 18), and subjected to pause in laying and injected every day with 200 μg/kg BW of chicken GH (chGH; n = 18). Read More

View Article and Full-Text PDF
October 2017

Protein Corona Analysis of Silver Nanoparticles Exposed to Fish Plasma.

Environ Sci Technol Lett 2017 May 7;4(5):174-179. Epub 2017 Apr 7.

Department of Forestry and Natural Resources and Bindley Biosciences Center, Purdue University, West Lafayette, Indiana, USA.

Nanoparticles (NPs) in contact with biological fluids experience changes in surface chemistry that can impact their biodistribution and downstream physiological impact. One such change involves the formation of a protein corona (PC) on the surface of NPs. Here we present a foundational study on PC formation following the incubation of polyvinylpyrrolidone-coated AgNPs (PVP-AgNPs, 50 nm) in the plasma of smallmouth bass (). Read More

View Article and Full-Text PDF

Performance of a polymer coated silicon microarray for simultaneous detection of food allergen-specific IgE and IgG.

Clin Exp Allergy 2017 Aug 9;47(8):1057-1068. Epub 2017 May 9.

Pediatric Pneumology and Immunology, Charité Universitätsmedizin, Berlin, Germany.

Background: Microarray-based component-resolved diagnostics (CRD) has become an accepted tool to detect allergen-specific IgE sensitization towards hundreds of allergens in parallel from one drop of serum. Nevertheless, specificity and sensitivity as well as a simultaneous detection of allergen-specific IgG , as a potential parameter for tolerance development, remain to be optimized.

Objective: We applied the recently introduced silicon chip coated with a functional polymer named copoly(DMA-NAS-MAPS) to the simultaneous detection of food allergen-specific IgE and IgG , and compared it with ImmunoCAP and ImmunoCAP ISAC. Read More

View Article and Full-Text PDF

A randomized trial of egg introduction from 4 months of age in infants at risk for egg allergy.

J Allergy Clin Immunol 2017 May 11;139(5):1621-1628.e8. Epub 2016 Oct 11.

Discipline of Paediatrics and Child Health, University of Sydney, Sydney, Australia; Department of Allergy and Immunology, Children's Hospital at Westmead, Sydney, Australia. Electronic address:

Background: Epidemiologic evidence suggests delayed introduction of egg might not protect against egg allergy in infants at risk of allergic disease.

Objective: We sought to assess whether dietary introduction of egg between 4 and 6 months in infants at risk of allergy would reduce sensitization to egg.

Methods: We conducted a randomized controlled trial in infants with at least 1 first-degree relative with allergic disease. Read More

View Article and Full-Text PDF

Effects of maternal dietary egg intake during early lactation on human milk ovalbumin concentration: a randomized controlled trial.

Clin Exp Allergy 2016 12 7;46(12):1605-1613. Epub 2016 Oct 7.

School of Paediatrics and Child Health, The University of Western Australia, Perth, WA, Australia.

Background: There is limited understanding of how maternal diet affects breastmilk food allergen concentrations, and whether exposure to allergens through this route influences the development of infant oral tolerance or sensitization.

Objective: To investigate how maternal dietary egg ingestion during early lactation influences egg protein (ovalbumin) levels detected in human breastmilk.

Methods: In a randomized controlled trial, women were allocated to a dietary group for the first six weeks of lactation: high-egg diet (> 4 eggs per week), low-egg diet (one-three eggs per week) or an egg-free diet. Read More

View Article and Full-Text PDF
December 2016

Randomized controlled trial of early regular egg intake to prevent egg allergy.

J Allergy Clin Immunol 2017 May 20;139(5):1600-1607.e2. Epub 2016 Aug 20.

Women's & Children's Health Research Institute, Adelaide, Australia; School of Medicine, University of Adelaide, Women's and Children's Health Network, Adelaide, Australia; Healthy Mothers, Babies and Children, South Australian Health and Medical Research Institute, Adelaide, Australia.

Background: The ideal age to introduce egg into the infant diet has been debated for the past 2 decades in the context of rising rates of egg allergy.

Objective: We sought to determine whether regular consumption of egg protein from age 4 to 6 months reduces the risk of IgE-mediated egg allergy in infants with hereditary risk, but without eczema.

Methods: Infants aged 4 to 6 months were randomly allocated to receive daily pasteurized raw whole egg powder (n = 407) or a color-matched rice powder (n = 413) to age 10 months. Read More

View Article and Full-Text PDF

Randomized placebo-controlled trial of hen's egg consumption for primary prevention in infants.

J Allergy Clin Immunol 2017 May 12;139(5):1591-1599.e2. Epub 2016 Aug 12.

Department of Paediatric Pneumology and Immunology, Charité-Universitätsmedizin, Berlin, Germany; Icahn School of Medicine at Mount Sinai, New York, NY. Electronic address:

Background: Hen's egg is the most common cause of food allergy in early childhood.

Objective: We investigated the efficacy and safety of early hen's egg introduction at age 4 to 6 months to prevent hen's egg allergy in the general population.

Methods: This randomized, placebo-controlled trial included 4- to 6-month-old infants who were not sensitized against hen's egg, as determined based on specific serum antibodies (IgE). Read More

View Article and Full-Text PDF

Serine protease P-IIc is responsible for the digestion of yolk proteins at the late stage of silkworm embryogenesis.

Insect Biochem Mol Biol 2016 07 29;74:42-9. Epub 2016 Apr 29.

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, China. Electronic address:

In silkworms, yolk proteins comprise vitellin, egg-specific protein and 30K proteins, which are sequentially degraded by endogenous proteases strictly regulated during embryogenesis. Although the process has been extensively investigated, there is still a gap in the knowledge about the degradation of silkworm yolk proteins on the last two days of embryonic development. In the present study, we isolated and purified a gut serine protease P-IIc, which demonstrated optimal activity at 25 °C and pH 11. Read More

View Article and Full-Text PDF

Predictive values of egg-specific IgE by two commonly used assay systems for the diagnosis of egg allergy in young children: a prospective multicenter study.

Allergy 2016 10 13;71(10):1435-43. Epub 2016 Jul 13.

Allergy Center and Institute for Clinical Research, Mie National Hospital, Tsu, Japan.

Background: Specific IgE (sIgE) is often used to predict oral food challenge (OFC) outcomes in food allergy, but interpretation of the results may vary depending on the assay method employed and the patient population tested. The aim of this study was to use two commercial assay systems to determine egg-sIgE values predictive of allergy within the most common populations treated at pediatric clinics.

Methods: In a multicenter prospective study, 433 children with suspected or confirmed egg allergy underwent oral challenge (OFC) using cooked egg (CE) and raw egg (RE) powders to diagnose either true allergy in 1-year-old (group A, n = 220) or tolerance in 2- to 6-year-old (group B, n = 213). Read More

View Article and Full-Text PDF
October 2016

The sperm phospholipase C-ζ and Ca2+ signalling at fertilization in mammals.

Biochem Soc Trans 2016 Feb;44(1):267-72

College of Biomedical and Life Sciences, Cardiff University, Cardiff CF14 4XN, U.K.

A series of intracellular oscillations in the free cytosolic Ca(2+) concentration is responsible for activating mammalian eggs at fertilization, thus initiating embryo development. It has been proposed that the sperm causes these Ca(2+) oscillations after membrane fusion by delivering a soluble protein into the egg cytoplasm. We previously identified sperm-specific phospholipase C (PLC)-ζ as a protein that can trigger the same pattern of Ca(2+) oscillations in eggs seen at fertilization. Read More

View Article and Full-Text PDF
February 2016

Identification and Expression Analysis of Upregulated Genes in the Resting Egg-Producing Water Flea (Daphnia pulex).

Zoolog Sci 2016 Feb;33(1):106-15

Department of Biology, School of Medicine, Kurume University, Fukuoka 830-0011, Japan.

Water fleas (Daphnia pulex) normally produce subitaneous eggs that initiate development immediately after oviposition. However, in response to habitat degradation, resting eggs are produced, which are enclosed in a sturdy outer envelope (ephippium) and can survive in harsh environments for an extended time. To understand the molecular mechanism underlying resting egg production in D. Read More

View Article and Full-Text PDF
February 2016

Elevated IL-5 and IL-13 responses to egg proteins predate the introduction of egg in solid foods in infants with eczema.

Clin Exp Allergy 2016 Feb;46(2):308-16

School of Paediatrics and Child Health, The University of Western Australia, Perth, WA, Australia.

Background: Egg allergy is a leading cause of food allergy in young infants; however, little is known about early allergen-specific T-cell responses which predate the presentation of egg allergy, and if these are altered by early egg exposure.

Objective: To investigate the early T-cell responses to multiple egg proteins in relation to patterns of egg exposure and subsequent IgE-mediated egg allergy.

Methods: Egg-specific T-cell cytokine responses (IL-5, IL-13, IL-10, IFNγ and TNFα) to ovomucoid (OM), ovalbumin (OVA), conalbumin (CON) and lysozyme (LYS) were measured in infants with eczema at 4 months of age (n = 40), before randomization to receive 'early egg' or a placebo as part of a randomized controlled trial (Australian New Zealand Clinical Trials Registry number 12609000415202) and at 12 months of age (n = 58), when IgE-mediated egg allergy was assessed by skin prick test and food challenge. Read More

View Article and Full-Text PDF
February 2016