Gene 2021 May 2;781:145541. Epub 2021 Mar 2.
Biomedical Sciences Program, University of Science and Technology, Zewail City of Science and Technology, October Gardens, 6th of October City, Giza 12578, Egypt; Department of Molecular Genetics, AGERI, ARC, Giza 12619, Egypt; National Biotechnology Network of Expertise (NBNE), Academy of Science Research and Technology (ASRT), 11334 Cairo, Egypt. Electronic address:
Understanding how promoters work in non-host cells is complex. Nonetheless, understanding this process is crucial while performing gene expression modulation studies. This study began with the process of constructing a shuttle vector with CMV and OpIE2 promoters in a tandem arrangement to achieve gene expression in both mammalian and insect cells, respectively. Read More