2,858 results match your criteria efflux capacity

The Capacity of APOB-Depleted Plasma in Inducing ATP-Binding Cassette A1/G1-Mediated Macrophage Cholesterol Efflux-But Not Gut Microbial-Derived Metabolites-Is Independently Associated with Mortality in Patients with ST-Segment Elevation Myocardial Infarction.

Biomedicines 2021 Sep 27;9(10). Epub 2021 Sep 27.

Department of Clinical Biochemistry, Hospital de la Santa Creu i Sant Pau, IIB Sant Pau, 08041 Barcelona, Spain.

Impaired HDL-mediated macrophage cholesterol efflux and higher circulating concentrations of trimethylamine N-oxide (TMAO) levels are independent risk factors for cardiovascular mortality. The TMAO precursors, γ-butyrobetaine (γBB) and Trimethyllysine (TML), have also been recently associated with cardiovascular death, but their interactions with HDL-mediated cholesterol efflux remain unclear. We aimed to determine the associations between APOB depleted plasma-mediated macrophage cholesterol efflux and plasma TMAO, γBB, and TML concentrations and explore their association with two-year follow-up mortality in patients with acute ST-elevation myocardial infarction (STEMI) and unstable angina (UA). Read More

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September 2021

ATP-Responsive Multifunctional Supramolecular Polymer as a Nonviral Vector for Boosting Cholesterol Removal from Lipid-Laden Macrophages.

ACS Biomater Sci Eng 2021 Oct 14. Epub 2021 Oct 14.

Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, PR China.

Specific delivery of NCEH1 plasmid is a promising approach to boost the cholesterol removal from lipid-laden macrophages for antiatherosclerosis. Polyethylenimine (PEI) is one of the most efficient gene carriers among nonviral vectors. However, the high transfection activity of PEI is always accompanied by profound cytotoxicity. Read More

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October 2021

Leisure time physical activity is associated with improved HDL functionality in high cardiovascular risk individuals: a cohort study.

Eur J Prev Cardiol 2021 Oct;28(12):1392-1401

Hospital del Mar Medical Research Institute (IMIM), Spain.

Aims: Physical activity has consistently been shown to improve cardiovascular health and high-density lipoprotein-cholesterol levels. However, only small and heterogeneous studies have investigated the effect of exercise on high-density lipoprotein functions. Our aim is to evaluate, in the largest observational study to date, the association between leisure time physical activity and a range of high-density lipoprotein functional traits. Read More

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October 2021

Transcriptome analysis reveals disruption of circadian rhythms in late gestation dairy cows may increase risk for fatty liver and reduced mammary remodeling.

Physiol Genomics 2021 Oct 13. Epub 2021 Oct 13.

Department of Animal Sciences, Purdue University, West Lafayette, IN, United States.

Circadian disruption increased insulin resistance and decreased mammary development in late gestation, non-lactating (dry) cows. The objective was to measure the effect of circadian disruption on transcriptomes of the liver and mammary gland. At 35 d before expected calving (BEC) multiparous dry cows were assigned to either control (CON) or phase-shifted treatments (PS). Read More

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October 2021

HDL and reverse cholesterol transport in humans and animals: Lessons from pre-clinical models and clinical studies.

Biochim Biophys Acta Mol Cell Biol Lipids 2021 Oct 9;1867(1):159065. Epub 2021 Oct 9.

Division of Translational Medicine & Human Genetics, Perelman School of Medicine at the University of Pennsylvania, 3600 Spruce Street, Philadelphia, PA 19104, USA.

The ability to accept cholesterol from cells and to promote reverse cholesterol transport (RCT) represents the best characterized antiatherogenic function of HDL. Studies carried out in animal models have unraveled the multiple mechanisms by which these lipoproteins drive cholesterol efflux from macrophages and cholesterol uptake to the liver. Moreover, the influence of HDL composition and the role of lipid transporters have been clarified by using suitable transgenic models or through experimental design employing pharmacological or nutritional interventions. Read More

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October 2021

Cross-Sectional Associations Between HDL Structure or Function, Cell Membrane Fatty Acid Composition, and Inflammation in Elderly Adults.

J Nutr 2021 Oct 12. Epub 2021 Oct 12.

Universitat Rovira i Virgili, Departament de Bioquímica i Biotecnologia, Unitat de Nutrició, Reus, Spain.

Background: Cell membrane fatty acid composition has been related to inflammation and cardiovascular risk. Dysregulation of HDL functionis also considered a cardiovascular risk factor.

Objective: We aimed to investigate whether the content of cell membrane fatty acids and HDL functionality are linked to each other as well as to inflammation. Read More

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October 2021

Efflux transporters in rat placenta and developing brain: transcriptomic and functional response to paracetamol.

Sci Rep 2021 Oct 6;11(1):19878. Epub 2021 Oct 6.

Department of Biochemistry & Pharmacology, University of Melbourne, Parkville, VIC, 3010, Australia.

Adenosine triphosphate binding cassette (ABC) transporters transfer lipid-soluble molecules across cellular interfaces either directly or after enzymatic metabolism. RNAseq analysis identified transcripts for ABC transporters and enzymes in rat E19, P5 and adult brain and choroid plexus and E19 placenta. Their functional capacity to efflux small molecules was studied by quantitative analysis of paracetamol (acetaminophen) and its metabolites using liquid scintillation counting, autoradiography and ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS). Read More

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October 2021

A lifestyle intervention with an energy-restricted Mediterranean diet and physical activity enhances HDL function: a substudy of the PREDIMED-Plus randomized controlled trial.

Am J Clin Nutr 2021 Sep 28. Epub 2021 Sep 28.

Cardiovascular Risk and Nutrition Research Group, Hospital del Mar Medical Research Institute, Barcelona, Spain.

Background: Consumption of a Mediterranean diet, adequate levels of physical activity, and energy-restricted lifestyle interventions have been individually associated with improvements in HDL functions. Evidence of intensive interventions with calorie restriction and physical activity is, however, scarce.

Objectives: To determine whether an intensive lifestyle intervention with an energy-restricted Mediterranean diet plus physical activity enhanced HDL function compared to a non-hypocaloric Mediterranean eating pattern without physical activity. Read More

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September 2021

Effects of Elaidic Acid on HDL Cholesterol Uptake Capacity.

Nutrients 2021 Sep 4;13(9). Epub 2021 Sep 4.

Division of Cardiovascular Medicine, Graduate School of Medicine, Kobe University, Kobe 650-0017, Japan.

Recently we established a cell-free assay to evaluate "cholesterol uptake capacity (CUC)" as a novel concept for high-density lipoprotein (HDL) functionality and demonstrated the feasibility of CUC for coronary risk stratification, although its regulatory mechanism remains unclear. HDL fluidity affects cholesterol efflux, and trans fatty acids (TFA) reduce lipid membrane fluidity when incorporated into phospholipids (PL). This study aimed to clarify the effect of TFA in HDL-PL on CUC. Read More

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September 2021

Participation of the ABC Transporter in Azole Resistance of .

J Fungi (Basel) 2021 Sep 15;7(9). Epub 2021 Sep 15.

Institute of Microbiology, University of Lausanne and University Hospital, CH-1011 Lausanne, Switzerland.

is an opportunistic pathogen in humans that causes infrequent but difficult-to-treat diseases. Antifungal drugs are used in the clinic to treat infections, however, this fungus can rapidly acquire antifungal resistance to all known antifungal drugs (multidrug resistance). acquires azole resistance by gain-of-function (GOF) mutations in the transcriptional regulator . Read More

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September 2021

Lysis reagents, cell numbers, and calculation method influence high-throughput measurement of HDL-mediated cholesterol efflux capacity.

J Lipid Res 2021 Sep 24:100125. Epub 2021 Sep 24.

Institute of Genetic Epidemiology, Department of Genetics and Pharmacology, Medical University of Innsbruck, Innsbruck, Austria. Electronic address:

HDL-mediated cholesterol efflux capacity (CEC) may protect against cardiovascular disease. However, CEC assays are not standardized, hampering their application in large cohorts and comparison between studies. To improve standardization, we systematically investigated technical differences between existing protocols that influence assay performance that have not been previously addressed. Read More

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September 2021

Investigation of the effective components inhibited macrophage foam cell formation in Ophiopogonis Radix.

J Ethnopharmacol 2021 Sep 23:114678. Epub 2021 Sep 23.

Research Center for Traceability and Standardization of TCMs, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 211198, PR China; Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 211198, PR China; State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 2111198, PR China. Electronic address:

Ethnopharmacological Relevance: Ophiopogonis Radix, the commonly used traditional Chinese medicines in clinic for treating cardiovascular diseases, is returned to the stomach, lung and heart meridian. It is reported to nourish yin, moisten lung and is used to treat heart yin deficiency syndromes and asthenia of heart and lung, which indicated that Ophiopogonis Radix may have a protective effect on heart disorders. Atherosclerosisis is an important process in the development of cardiovascular disease and abnormal lipid deposition induced macrophage foam cells is its important foundation. Read More

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September 2021

RNA-seq-based transcriptome analysis of a cefquinome-treated, highly resistant, and virulent MRSA strain.

Microb Pathog 2021 Nov 20;160:105201. Epub 2021 Sep 20.

MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address:

The emergence and dissemination of methicillin-resistant Staphylococcus aureus (MRSA) strains of animal origin that are resistant to several antibiotics is of great concern. Cefquinome is a fourth-generation cephalosporin developed specifically for veterinary use. The mechanism of MRSA resistance to cefquinome is still not established. Read More

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November 2021

Cholesterol efflux capacity and its association with prevalent metabolic syndrome in a multi-ethnic population (Dallas Heart Study).

PLoS One 2021 21;16(9):e0257574. Epub 2021 Sep 21.

Division of Cardiology, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America.

Metabolic syndrome (MetS) is characterized by adiposity and atherogenic dyslipidemia consisting of elevated triglyceride and decreased high density lipoprotein cholesterol (HDL-C) levels however, cholesterol concentration alone does not reflect HDL functionality. Cholesterol efflux capacity (CEC) captures a key anti-atherosclerotic function of HDL; studies linking CEC to MetS have yielded inconsistent findings and lacked racial/ethnic diversity. The aim of this study was to evaluate the association between CEC and MetS in a large multi-ethnic population utilizing two different CEC assays interrogating overlapping but distinct reverse cholesterol transport pathways. Read More

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September 2021

Liposome interaction with macrophages and foam cells for atherosclerosis treatment: effects of size, surface charge and lipid composition.

Nanotechnology 2021 Oct 7;32(50). Epub 2021 Oct 7.

School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Ave, 639798, Singapore.

Liposomes are potential drug carriers for atherosclerosis therapy due to low immunogenicity and ease of surface modifications that allow them to have prolonged circulation half-life and specifically target atherosclerotic sites to increase uptake efficiency. However, the effects of their size, charge, and lipid compositions on macrophage and foam cell behaviour are not fully understood. In this study, liposomes of different sizes (60 nm, 100 nm and 180 nm), charges (-40 mV, -20 mV, neutral, +15 mV and +30 mV) and lipid compositions (1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, L-a-phosphatidylcholine, and egg sphingomyelin) were synthesized, characterized and exposed to macrophages and foam cells. Read More

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October 2021

Reversing Multidrug Resistance by Inducing Mitochondrial Dysfunction for Enhanced Chemo-Photodynamic Therapy in Tumor.

ACS Appl Mater Interfaces 2021 Sep 17;13(38):45259-45268. Epub 2021 Sep 17.

State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, Hi-tech Zone, Dalian 116024, P.R. China.

Efficiency of standard chemotherapy is dramatically hindered by intrinsic multidrug resistance (MDR). Recently, to amplify therapeutic efficacy, photodynamic therapy (PDT)-induced mitochondrial dysfunction by decorating targeting moieties on nanocarriers has obtained considerable attention. Nevertheless, low targeting efficiency, complex synthesis routes, and difficulty in releasing contents become the major obstacles in further clinical application. Read More

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September 2021

Novel nanomedicines to overcome cancer multidrug resistance.

Drug Resist Updat 2021 Sep 4;58:100777. Epub 2021 Aug 4.

Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, 518001, Guangdong, PR China; Shenzhen Public Service Platform on Tumor Precision Medicine and Molecular Diagnosis, Shenzhen, 518001, Guangdong, PR China. Electronic address:

Chemotherapy remains a powerful tool to eliminate malignant cells. However, the efficacy of chemotherapy is compromised by the frequent emergence of intrinsic and acquired multidrug resistance (MDR). These chemoresistance modalities are based on a multiplicity of molecular mechanisms of drug resistance, including : 1) Impaired drug uptake into cancer cells; 2) Increased expression of ATP-binding cassette efflux transporters; 3) Loss of function of pro-apoptotic factors; 4) Enhanced DNA repair capacity; 5) Qualitative or quantitative alterations of specific cellular targets; 6) Alterations that allow cancer cells to tolerate adverse or stressful conditions; 7) Increased biotransformation or metabolism of anticancer drugs to less active or completely inactive metabolites; and 8) Intracellular and intercellular drug sequestration in well-defined organelles away from the cellular target. Read More

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September 2021

A Unique Sequence Is Essential for Efficient Multidrug Efflux Function of the MtrD Protein of .

mBio 2021 08 31;12(4):e0167521. Epub 2021 Aug 31.

College of Science and Engineering, Flinders Universitygrid.1014.4, Bedford Park, SA, Australia.

Antimicrobial resistance in Neisseria gonorrhoeae has reached an alarming level, severely impacting the effective treatment of gonorrhea. Belonging to the resistance-nodulation-cell division (RND) superfamily of efflux transporters, the MtrD membrane protein of N. gonorrhoeae provides resistance to a broad range of antimicrobial compounds. Read More

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Influence of low temperature on comparative arsenic accumulation and release by three hyperaccumulators.

J Environ Sci Health A Tox Hazard Subst Environ Eng 2021 Aug 27:1-10. Epub 2021 Aug 27.

Graduate School of Environmental Studies (GSES), Tohoku University, Sendai, Japan.

Arsenic (As) hyperaccumulator ferns are renowned for their capacity to accumulate As and have been used to remediate As-contaminated environmental. However, there is less information on how they perform under low temperature though it is important for practical phytoremediation. The purpose of this study was to identify the effect of temperature on As accumulation by three As hyperaccumulators, , and . Read More

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Cholesterol Efflux Capacity Associates with the Ankle-Brachial Index but Not All-Cause Mortality in Patients with Peripheral Artery Disease.

Diagnostics (Basel) 2021 Aug 4;11(8). Epub 2021 Aug 4.

Institute of Clinical Chemistry, University Hospital Zurich, University of Zurich, Rämistrasse 100, CH-8091 Zurich, Switzerland.

Background: Cholesterol efflux is an important mechanism by which high-density lipoproteins (HDLs) protect against cardiovascular disease. As peripheral artery disease (PAD) is associated with high mortality rates, mainly due to cardiovascular causes, we investigated whether cholesterol efflux capacity (CEC) of apolipoprotein B (apoB)-depleted plasma, a widely used surrogate of HDL function, may serve as a predictive marker for mortality in this patient population.

Methods: In this prospective single-center study (median follow-up time: 9. Read More

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Phosphorus-Induced Adaptation Mechanisms of Rye Grown on Post-Flotation Copper Tailings.

Biology (Basel) 2021 Aug 23;10(8). Epub 2021 Aug 23.

Department of Plant Nutrition, Wroclaw University of Environmental and Life Sciences, ul. Grunwaldzka 53, 50-357 Wroclaw, Poland.

Although a considerable effort has been made over the last decades to develop cost-effective phytotechnologies as an alternative to conventional techniques for the management of contaminated lands, successful revegetation of the tailings still represents a major challenge. Here, we evaluate the potential of rye ( L.) for growth and survival on the tailings after copper (Cu) ore processing. Read More

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Metagenomics-Based Analysis of the Age-Related Cumulative Effect of Antibiotic Resistance Genes in Gut Microbiota.

Antibiotics (Basel) 2021 Aug 20;10(8). Epub 2021 Aug 20.

Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.

Antibiotic resistance in bacteria has become a major global health problem. One of the main reservoirs of antibiotic resistance genes is the human gut microbiota. To characterise these genes, a metagenomic approach was used. Read More

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Molecular Patterns of Extreme and Persistent Cholesterol Efflux Capacity.

Arterioscler Thromb Vasc Biol 2021 Oct 26;41(10):2588-2597. Epub 2021 Aug 26.

Department of Internal Medicine, Division of Cardiology. University of Texas Southwestern Medical Center, Dallas (A.E.-G., S.D., S.S., B.S., A.G., M.P., A.R.).

[Figure: see text]. Read More

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October 2021

Ketoconazole resistant Candida albicans is sensitive to a wireless electroceutical wound care dressing.

Bioelectrochemistry 2021 Dec 4;142:107921. Epub 2021 Aug 4.

Indiana Center for Regenerative Medicine & Engineering, Indiana University Health Comprehensive Wound Center, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, United States. Electronic address:

Wireless electroceutical dressing (WED) fabric kills bacteria and disrupts bacterial biofilm. This work tested, comparing with standard of care topical antibiotic ketoconazole, whether the weak electric field generated by WED is effective to manage infection caused by ketoconazole-resistant yeast Candida albicans. WED inhibited Candida albicans biofilm formation and planktonic growth. Read More

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December 2021

Serum hepcidin / ferroportin levels in bipolar disorder and schizophrenia.

J Trace Elem Med Biol 2021 Aug 14;68:126843. Epub 2021 Aug 14.

Department of Biochemistry, Ankara Yıldırım Beyazıt Univerisity, Faculty of Medicine, Bilkent Road 3. Km., Çankaya, Ankara, Turkey. Electronic address:

Background: Despite several alternatives for cellular iron influx, the only mechanism for cellular iron efflux is ferroportin mediated active transport. In cases of ferroportin dysfunction, iron accumulates in the cell and causes ferroptosis. Hepcidin suppresses ferroportin levels and inflammatory activation increases hepcidin production. Read More

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Seedling growth and physicochemical transformations of rice nursery soil under varying levels of coal fly ash and vermicompost amendment.

Environ Geochem Health 2021 Aug 17. Epub 2021 Aug 17.

Soil Testing Laboratory, Sonepur, Odisha, India.

Fly ash is an inevitable by-product from the coal-fired power plants in many developing countries including India that needs safe, timely and productive disposal. The addition of fly ash alters physicochemical properties of soil and hence could be used as a soil conditioner or modifier along with the appropriate level of vermicompost to support plant growth. Several studies have focalized sole use of fly ash and vermicompost in agricultural production systems lacking information on combined application effects. Read More

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AMP deamination is sufficient to replicate an atrophy-like metabolic phenotype in skeletal muscle.

Metabolism 2021 Oct 13;123:154864. Epub 2021 Aug 13.

Indiana Center for Musculoskeletal Health, Indiana University School of Medicine, Indianapolis, IN, USA; Department of Anatomy, Cell Biology & Physiology, Indiana University School of Medicine, Indianapolis, IN, USA. Electronic address:

Background: Skeletal muscle atrophy, whether caused by chronic disease, acute critical illness, disuse or aging, is characterized by tissue-specific decrease in oxidative capacity and broad alterations in metabolism that contribute to functional decline. However, the underlying mechanisms responsible for these metabolic changes are largely unknown. One of the most highly upregulated genes in atrophic muscle is AMP deaminase 3 (AMPD3: AMP → IMP + NH), which controls the content of intracellular adenine nucleotides (AdN; ATP + ADP + AMP). Read More

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October 2021

Matcha Green Tea Powder does not Prevent Diet-Induced Arteriosclerosis in New Zealand White Rabbits Due to Impaired Reverse Cholesterol Transport.

Mol Nutr Food Res 2021 Oct 27;65(20):e2100371. Epub 2021 Aug 27.

Department of Internal Medicine, Medical University of Innsbruck, Innsbruck, Austria.

Introduction: Green tea is associated with decreased risk for cardiovascular disease and stroke. Matcha is a special kind of powdered green tea known for its use in the Japanese tea ceremony. Due to its influence on lipoprotein parameters, it has been postulated to exert antiatherogenic effects. Read More

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October 2021

Associations of endogenous hormones with HDL novel metrics across the menopause transition: The SWAN HDL Study.

J Clin Endocrinol Metab 2021 Aug 14. Epub 2021 Aug 14.

Department of Epidemiology, University of Pittsburgh, Graduate School of Public Health Pittsburgh PA.

Context: Novel metrics of HDL (subclasses, lipid content and function) may better characterize the anti-atherogenic features of HDL. In midlife women, changes in these metrics vary by time relative to the final menstrual period (FMP), supporting a contribution of menopause hormones estradiol (E2) and follicle-stimulating hormone (FSH).

Objective: To test associations of endogenous E2 and FSH with novel HDL metrics and to assess whether these associations vary by time relative to FMP. Read More

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Strength of Cu-efflux response in Escherichia coli coordinates metal resistance in Caenorhabditis elegans and contributes to the severity of environmental toxicity.

J Biol Chem 2021 Sep 8;297(3):101060. Epub 2021 Aug 8.

Molecular Toxicology Interdepartmental Program, University of California, Los Angeles, Los Angeles, California, USA; Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California, USA; Institute for Society and Genetics, University of California, Los Angeles, Los Angeles, California, USA; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, California, USA. Electronic address:

Without effective homeostatic systems in place, excess copper (Cu) is universally toxic to organisms. While increased utilization of anthropogenic Cu in the environment has driven the diversification of Cu-resistance systems within enterobacteria, little research has focused on how this change in bacterial architecture impacts host organisms that need to maintain their own Cu homeostasis. Therefore, we utilized a simplified host-microbe system to determine whether the efficiency of one bacterial Cu-resistance system, increasing Cu-efflux capacity via the ubiquitous CusRS two-component system, contributes to the availability and subsequent toxicity of Cu in host Caenorhabditis elegans nematode. Read More

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September 2021