39,041 results match your criteria efficiencies complex

The Ability of Probiotic Lactobacillus Strains in Removal of Benzo[a]pyrene: a Response Surface Methodology Study.

Probiotics Antimicrob Proteins 2021 Jun 15. Epub 2021 Jun 15.

Department of Food Science and Technology, Faculty of Nutrition Sciences and Food Technology, National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

In this study, the ability of various lactic acid bacteria was assessed in removing benzo[a]pyrene (BaP) from contaminated phosphate buffer saline (PBS). Response surface methodology (RSM) was performed using Box-Behnken design to investigate the effect of four independent variables including pH (5-7), incubation time (1-24 h), cell density (10-10 cfu/mL), and initial BaP concentration (5-15 mg/kg) at three levels to evaluate in vitro removal of BaP as response. The results showed that all the tested strains were able to remove BaP from PBS and this reduction was entirely strain-specific. Read More

View Article and Full-Text PDF

Bi-polymeric Spongy Matrices Through Cross-linking Polymerization: Synthesized and Evaluated for Solubility Enhancement of Acyclovir.

AAPS PharmSciTech 2021 Jun 15;22(5):181. Epub 2021 Jun 15.

Department of Pharmaceutics, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, Punjab, Pakistan.

In this study, two hydrophilic polymers hydroxypropyl methyl cellulose and beta-cyclodextrin (β-CD) are used to synthesize highly responsive and spongy polymeric matrices. Porous and stimulus-responsive polymeric network was developed to improve the solubility of acyclovir (ACV) at significant level. Grafting was successfully carried out by free radical polymerization technique. Read More

View Article and Full-Text PDF

Robotic-assisted percutaneous iliac screw fixation for destructive lumbosacral metastatic lesions: an early single-institution experience.

Acta Neurochir (Wien) 2021 Jun 15. Epub 2021 Jun 15.

Division of Spine, Department of Neurosurgery, Duke University Medical Center, 200 Trent Drive, Durham, NC, 27710, USA.

Background: Robotic-assisted surgery is becoming more widely applied in surgical subspecialties due to its intraoperative and postoperative advantages such as minimally invasive approach, reduced blood loss, shorter hospital stay, and decreased incidence of postoperative complications. However, robotic devices were only recently introduced in the field of spinal surgery. Specifically, percutaneous approaches involving computer-assisted image guidance are relatively new in iliac screw fixation. Read More

View Article and Full-Text PDF

One-Shot Neural Architecture Search by Dynamically Pruning Supernet in Hierarchical Order.

Int J Neural Syst 2021 Jun 14:2150029. Epub 2021 Jun 14.

College of Computer Science, Sichuan University, Section 4, Southern 1st Ring Rd, Chengdu, Sichuan 610065, P. R. China.

Neural Architecture Search (NAS), which aims at automatically designing neural architectures, recently draw a growing research interest. Different from conventional NAS methods, in which a large number of neural architectures need to be trained for evaluation, the one-shot NAS methods only have to train one supernet which synthesizes all the possible candidate architectures. As a result, the search efficiency could be significantly improved by sharing the supernet's weights during the candidate architectures' evaluation. Read More

View Article and Full-Text PDF

Mutation of aspartic acid 199 in USP1 disrupts its deubiquitinating activity and impairs DNA repair.

FEBS Lett 2021 Jun 15. Epub 2021 Jun 15.

Department of Pharmacology, Chonnam National University Medical School, Jellanamdo, 58128, Republic of Korea.

The deubiquitinating enzyme USP1 contains highly conserved motifs forming its catalytic center. Recently, the COSMIC mutation database identified a mutation in USP1 at Asp-199 in endometrial cancer. Here, we investigated the role of Asp-199 for USP1 function. Read More

View Article and Full-Text PDF

Heuristic optimization of impeller sidewall gaps-based on the bees algorithm for a centrifugal blood pump by CFD.

Int J Artif Organs 2021 Jun 15:3913988211023773. Epub 2021 Jun 15.

Faculty of Engineering and Natural Science, Mechanical Engineering Department, Konya Technical University, Konya, Turkey.

Optimization studies on blood pumps that require complex designs are gradually increasing in number. The essential design criteria of centrifugal blood pump are minimum shear stress with maximal efficiency. The geometry design of impeller sidewall gaps (blade tip clearance, axial gap, radial gap) is highly effective with regard to these two criteria. Read More

View Article and Full-Text PDF

Tumor microenvironment-triggered cancer vaccines inducing dual immunogenic cell death for elevated antitumor and antimetastatic therapy.

Nanoscale 2021 Jun 15. Epub 2021 Jun 15.

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China. and University of Science and Technology of China, Hefei, 230026, China.

Cancer vaccines are made from tumor-specific antigens, which are then injected back into the body to activate immune responses for cancer immunotherapy. Despite the high specificity and therapeutic efficiency, the vaccine has huge challenges such as complex preparation process, expensiveness and limited durational effects. Herein, a strategy to develop in situ cancer vaccines by enhancing the immunomodulatory effects for immunogenic cell death (ICD) is presented. Read More

View Article and Full-Text PDF

Cryo-EM structures of Lassa and Machupo virus polymerases complexed with cognate regulatory Z proteins identify targets for antivirals.

Nat Microbiol 2021 Jun 14. Epub 2021 Jun 14.

Savaid Medical School, University of Chinese Academy of Sciences, Beijing, China.

Zoonotic arenaviruses can lead to life-threating diseases in humans. These viruses encode a large (L) polymerase that transcribes and replicates the viral genome. At the late stage of replication, the multifunctional Z protein interacts with the L polymerase to shut down RNA synthesis and initiate virion assembly. Read More

View Article and Full-Text PDF

Modulating Cas9 activity for precision gene editing.

Prog Mol Biol Transl Sci 2021 18;181:89-127. Epub 2021 Mar 18.

Graduate School of Natural and Applied Sciences, Yeditepe University, Istanbul, Turkey; Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Istanbul, Turkey. Electronic address:

The CRISPR/Cas9 is a RNA-guided nuclease complex that can be specifically programmed to target a user-specified DNA sequence. It has been a powerful and effective tool of genome editing. However, off-target activity of the Cas9 nuclease limits its potential use in the correction of inherited diseases and bona fide gene editing. Read More

View Article and Full-Text PDF

Enhanced solubility of Albendazole in Cyclodextrin Inclusion Complex: A Molecular Modeling Approach and Physicochemical Evaluation.

Curr Drug Deliv 2021 Jun 13. Epub 2021 Jun 13.

Laboratório de Tecnologia dos Medicamentos, Universidade Federal de Pernambuco, Recife/PE, Brazil.

Background: Albendazole (ABZ) is the drug of choice for the treatment of a variety of human and veterinary parasites. However, it has low aqueous solubility and low bioavailability. Cyclodextrins (CD) are pharmaceutical excipients with the ability to modulate the solubilization property of hydrophobic molecules. Read More

View Article and Full-Text PDF

Regio- and diastereoselective dearomatizations of -alkyl activated azaarenes: the maximization of the reactive sites.

Chem Sci 2019 Dec 17;11(5):1418-1424. Epub 2019 Dec 17.

College of Chemistry and Chemical Engineering, Henan University Kaifeng 475004 China

An unprecedented base-promoted multi-component one-pot dearomatization of -alkyl activated azaarenes was developed, which enabled the synthesis of complex and diverse bridged cyclic polycycles with multiple stereocenters in a highly regio- and diastereoselective manner. Besides, we realized the step-controlled dearomative bi- and trifunctionalization of quinolinium salts. These transformations not only achieved the maximization of the reaction sites of pyridinium, quinolinium and isoquinolinium salts to enhance structural complexity and diversity, but also opened up a new reaction mode of these -activated azaarenes. Read More

View Article and Full-Text PDF
December 2019

Transmembrane anion transport mediated by halogen bonding and hydrogen bonding triazole anionophores.

Chem Sci 2020 Apr 15;11(18):4722-4729. Epub 2020 Apr 15.

Chemistry Research Laboratory, University of Oxford Mansfield Road Oxford OX1 3TA UK

Transmembrane ion transport by synthetic anionophores is typically achieved using polar hydrogen bonding anion receptors. Here we show that readily accessible halogen and hydrogen bonding 1,2,3-triazole derivatives can efficiently mediate anion transport across lipid bilayer membranes with unusual anti-Hofmeister selectivity. Importantly, the results demonstrate that the iodo-triazole systems exhibit the highest reported activity to date for halogen bonding anionophores, and enhanced transport efficiency relative to the hydrogen bonding analogues. Read More

View Article and Full-Text PDF

A highly X-ray sensitive iridium prodrug for visualized tumor radiochemotherapy.

Chem Sci 2020 Apr 1;11(15):3780-3789. Epub 2020 Apr 1.

Department of Chemistry, Jinan University Guangzhou 510632 China

Concomitant treatment of radiotherapy and chemotherapy is widely used in cancer therapy. The search for highly efficient radiochemotherapy drugs for tumor targeting therapy under image-guiding is of considerable interest. Herein we report an Ir-based prodrug with high sensitization efficiency for tumor microenvironment responsive cancer-targeted bioimaging radiochemotherapy. Read More

View Article and Full-Text PDF

Improving the sustainability of a reverse supply chain system under demand uncertainty by using postponement strategies.

Waste Manag 2021 Jun 11;131:72-87. Epub 2021 Jun 11.

Department of Industrial Engineering and Engineering Management, Mapúa University, Intramuros, Metro Manila, Philippines.

In recent decades, issues of resource depletion and waste piling have grown at an alarming rate, which are happening in the cases of product wastes with significant residual values, such as e-waste. To address these issues, stakeholders have focused to develop a reverse supply chain (RSC) system that can sustain profitable takeback, reuse, and recycling operations in the long-term. Such a system requires efficiency in handling complex operations involving various players while being responsive to demand uncertainty and changes. Read More

View Article and Full-Text PDF

Modular Synthesis of Amphiphilic Chitosan Derivatives Based on Copper-Free Click Reaction for Drug Delivery.

Int J Pharm 2021 Jun 11:120798. Epub 2021 Jun 11.

State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Drug Discovery for Metabolic Diseases, Center of Advanced Pharmaceuticals and Biomaterials, China Pharmaceutical University, Nanjing 210009, P.R. China. Electronic address:

Amphiphilic chitosan derivatives have attracted wide attention as drug carriers due to their physicochemical properties. However, obtaining a desired amphiphilic chitosan derivative by tuning the various functional groups was complex and time-consuming. Therefore, a facile and common synthesis strategy would be promising. Read More

View Article and Full-Text PDF

Simple MoS-Nanofiber Paper-Based Fluorescence Immunosensor for Point-of-Care Detection of Programmed Cell Death Protein 1.

Anal Chem 2021 Jun 14. Epub 2021 Jun 14.

Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Collaborative Innovation Center for Advanced Organic Chemical Materials Co-constructed by the Province and Ministry, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, P. R. China.

Programmed cell death protein 1 (PD-1) is one of the coinhibitory checkpoints upon T cell activation, the abnormal expression of which severely threatens host immune modulatation for chronic infection. Thus, fast and sensitive monitoring of PD-1 is of vital importance for early diagnosis and cancer treatment. The current detection methods largely based on enzyme-linked immunosorbent assay (ELISA) require time-consuming incubation and complicated washing steps. Read More

View Article and Full-Text PDF

Cholinergic anti-inflammatory pathway and connective tissue diseases.

Inflammopharmacology 2021 Jun 14. Epub 2021 Jun 14.

Immunology Research Center, Tabriz University of Medical Sciences, Golgasht St, Postcode: 5166614766, Tabriz, Iran.

Connective tissue diseases (CTDs) consist of an extensive range of heterogeneous medical conditions, which are caused by immune-mediated chronic inflammation and influences the various connective tissues of the body. They include rheumatoid arthritis, systemic lupus erythematosus, systemic sclerosis, vasculitis, Sjögren's syndrome, Behcet's disease, and many other autoimmune CTDs. To date, several anti-inflammatory approaches have been developed to reduce the severity of inflammation or its subsequent organ manifestations. Read More

View Article and Full-Text PDF

A One-Two-Three Multifunctional System for Enhanced Imaging and Detection of Intracellular MicroRNA and Chemogene Therapy.

ACS Appl Mater Interfaces 2021 Jun 14. Epub 2021 Jun 14.

Weifang Customs, Yuqing East Street, No.15789, High tech District, Weifang 261000, Shandong Province, China.

Simultaneous imaging, diagnosis, and therapy can offer an effective strategy for cancer treatment. However, the complex probe design, poor drug release efficiency, and multidrug resistance remain tremendous challenges to cancer treatment. Here, a novel one-two-three system is built for enhanced imaging and detection of miRNA-21 (miR-21) overexpressed in cancer cell and chemogene therapy. Read More

View Article and Full-Text PDF

Dual-Emission Ratiometric Fluorescent Probe Based on Lanthanide-Functionalized Carbon Quantum Dots for White Light Emission and Chemical Sensing.

ACS Omega 2021 Jun 27;6(22):14629-14638. Epub 2021 May 27.

School of Materials Science & Engineering, University of Shanghai for Science and Technology, Shanghai 200093, P. R. China.

Herein, we develop a novel method to synthesize lanthanide-functionalized carbon quantum dots via free-radical copolymerization using the methyl methacrylate (MMA) monomer as a functional monomer and introducing a lanthanide complex to obtain the dual-emission fluorescent composite material FCQDs-Ln(TFA) (Ln = Eu, Tb; TFA: trifluoroacetylacetone). The obtained composites were fully characterized, and their structures were investigated by Fourier transform infrared spectroscopy (FTIR), H NMR spectroscopy, X-ray photoelectron spectroscopy (XPS), and transmission electron microscopy (TEM). Subsequently, a series of white-light-emitting polymer composite films FCQDs- (Eu:Tb)(TFA)/poly(methyl methacrylate) (PMMA) were designed and synthesized by adjusting the ratio of Eu(TFA)/Tb(TFA) under different wavelengths. Read More

View Article and Full-Text PDF

Production of Phenotypically Uniform Human Cerebral Organoids from Pluripotent Stem Cells.

Bio Protoc 2021 Apr 20;11(8):e3985. Epub 2021 Apr 20.

Department of Biochemistry, Donnelly Centre, University of Toronto, Toronto, ON, Canada.

Recent advances in stem cell technology have allowed researchers to generate 3D cerebral organoids (COs) from human pluripotent stem cells (hPSCs). Indeed, COs have provided an unprecedented opportunity to model the developing human brain in a 3D context, and in turn, are suitable for addressing complex neurological questions by leveraging advancements in genetic engineering, high resolution microscopy, and tissue transcriptomics. However, the use of this model is limited by substantial variations in the overall morphology and cellular composition of organoids derived from the same pluripotent cell line. Read More

View Article and Full-Text PDF

Modified cyclodextrins solubilize elemental sulfur in water and enable biological sulfane sulfur delivery.

Chem Sci 2020 Oct 5;11(43):11777-11784. Epub 2020 Oct 5.

Department of Chemistry and Biochemistry, Materials Science Institute, Knight Campus for Accelerating Scientific Impact, Institute of Molecular Biology, University of Oregon Eugene OR 97403 USA

An important form of biological sulfur is sulfane sulfur, or S, which is found in polysulfide and persulfide compounds as well as in elemental sulfur. Sulfane sulfur, often in the form of S, functions as a key energy source in the metabolic processes of thermophilic Archaean organisms found in sulfur-rich environments and can be metabolized both aerobically and anaerobically by different archaeons. Despite this importance, S has a low solubility in water (∼19 nM), raising questions of how it can be made chemically accessible in complex environments. Read More

View Article and Full-Text PDF
October 2020

Molecular Basis for the Interactions of Human Thioredoxins with Their Respective Reductases.

Oxid Med Cell Longev 2021 1;2021:6621292. Epub 2021 Jun 1.

Institute for Medical Biochemistry and Molecular Biology, University Medicine Greifswald, Germany.

The mammalian cytosolic thioredoxin (Trx) system consists of Trx1 and its reductase, the NADPH-dependent seleno-enzyme TrxR1. These proteins function as electron donor for metabolic enzymes, for instance in DNA synthesis, and the redox regulation of numerous processes. In this work, we analysed the interactions between these two proteins. Read More

View Article and Full-Text PDF

Scaling of heart rate with breathing frequency and body mass in cetaceans.

Philos Trans R Soc Lond B Biol Sci 2021 Aug 14;376(1830):20200223. Epub 2021 Jun 14.

Fundación Oceanogràfic de la Comunitat Valenciana, Valencia, Spain 46005.

Plasticity in the cardiac function of a marine mammal facilitates rapid adjustments to the contrasting metabolic demands of breathing at the surface and diving during an extended apnea. By matching their heart rate () to their immediate physiological needs, a marine mammal can improve its metabolic efficiency and maximize the proportion of time spent underwater. Respiratory sinus arrhythmia (RSA) is a known modulation of that is driven by respiration and has been suggested to increase cardiorespiratory efficiency. Read More

View Article and Full-Text PDF

A single nucleotide substitution in the SPDT transporter gene reduced phytic acid and increased mineral bioavailability from Rice grain (Oryza sativa L.).

J Food Biochem 2021 Jun 13:e13822. Epub 2021 Jun 13.

Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India.

Phosphorus (P) flow in agricultural land depends on the P taken off from harvested product, its losses through runoff and fertilizer applied to balance the removed P. Phytic acid (PA), the major storage form of phosphorus (P) in cereal grains is a key anti-nutrient for human and non-ruminants leads to eutrophication of waterways. As the natural non-renewable P reserves are limited, enhancing P use efficiency is needed for field crops. Read More

View Article and Full-Text PDF

Logistics management provides greater efficiency, governance and compliance.

Int J Clin Pharm 2021 Jun 13. Epub 2021 Jun 13.

Pharmaceutical department, Usl Umbria 1, A.Migliorati street, 06132, Perugia, Italy.

During the 2020 Congress of the Italian Society of Hospital Pharmacy (Sifo 2020), there was a heated debate about whether it is more convenient to dispense healthcare directly in hospitals and public facilities, or indirectly, through the use of external private pharmacies. The former solution is called "direct dispensing" (DD), while the latter is called "dispensing on commission" (DPC). The strengths and advantages of DD over DPC are many: greater therapeutic appropriateness due to direct control of treatment plans, cost savings from external commissions, and greater clinical sharing of treatment by all healthcare personnel. Read More

View Article and Full-Text PDF

Associations between behaviour and health outcomes in conventional and slow-growing breeds of broiler chicken.

Animal 2021 Jun 10;15(7):100261. Epub 2021 Jun 10.

The Royal Veterinary College, Hawkshead Lane, Brookmans Park, Hatfield AL9 7TA, United Kingdom.

Broiler chickens are prone to a range of complex health and welfare issues. To support informed selection of welfare traits whilst minimising impact on production efficiency and to address a major gap in understanding, we systematically explored associations between health and behavioural indicators of broiler welfare. One conventional (CNV, n = 350) and two slow-growing broiler breeds (SGH and SGN, respectively n = 400) were reared from hatch in pens of 50 birds. Read More

View Article and Full-Text PDF

Unplanted wetland-type filter for co-treatment of landfill leachate and septic tank wastewater: Analysing gravel replacement by plastic and passive (filling-emptied) aeration effects at pilot scale.

J Environ Manage 2021 Jun 10;294:112940. Epub 2021 Jun 10.

Department of Sciences and Techniques of Water and the Environment, School of Civil Engineering, University of Cantabria, Santander, Spain.

Nowadays, traditional residential and industrial wastewater treatment methods have been mainly developed as complex systems that consider costly infrastructure, which requires advanced control systems and highly qualified labour for their operation. The use of wetland-type infrastructure has been recognized both, by scientists and authorities, as an efficient and effective method to obtain good results in these processes. The most relevant elements in the design of horizontal subsurface filters are filtering media, as biofilm supporting material and flow control methods. Read More

View Article and Full-Text PDF

Fabrication and applications of bioactive chitosan-based organic-inorganic hybrid materials: A review.

Carbohydr Polym 2021 Sep 8;267:118179. Epub 2021 May 8.

MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. Electronic address:

Organic-inorganic hybrid materials like bone, shells, and teeth can be found in nature, which are usually composed of biomacromolecules and nanoscale inorganic ingredients. Synergy of organic-inorganic components in hybrid materials render them outstanding and versatile performance. Chitosan is commonly used organic materials in bionic hybrid materials since its bioactive properties and could be controllable tailored by various means to meet complex conditions in different applications. Read More

View Article and Full-Text PDF
September 2021

MIDeepSeg: Minimally interactive segmentation of unseen objects from medical images using deep learning.

Med Image Anal 2021 May 18;72:102102. Epub 2021 May 18.

School of Mechanical and Electrical Engineering, University of Electronic Science and Technology of China, Chengdu, China; SenseTime Research, Shanghai, China.

Segmentation of organs or lesions from medical images plays an essential role in many clinical applications such as diagnosis and treatment planning. Though Convolutional Neural Networks (CNN) have achieved the state-of-the-art performance for automatic segmentation, they are often limited by the lack of clinically acceptable accuracy and robustness in complex cases. Therefore, interactive segmentation is a practical alternative to these methods. Read More

View Article and Full-Text PDF

The murburn precepts for aerobic respiration and redox homeostasis.

Prog Biophys Mol Biol 2021 Jun 9. Epub 2021 Jun 9.

Institute of Chemical Kinetics and Combustion Russian Academy of Sciences, St. Institutskaya 3, 630090, Novosibirsk, Russia. Electronic address:

Murburn concept is a new perspective to metabolism which posits that certain redox enzymes/proteins mediate catalysis outside their active site, via diffusible reactive oxygen species (DROS, usually deemed as toxic wastes). We have recently questioned the proton-centric chemiosmotic rotary ATP synthesis (CRAS) explanation for mitochondrial oxidative phosphorylation (mOxPhos) and proposed an oxygen-centric murburn model in lieu. Herein, the chemical equations and thermodynamic foundations for this new model of mOxPhos are detailed. Read More

View Article and Full-Text PDF