135 results match your criteria effects acidic-hydrolysis

Antioxidant and Hepatoprotective Effects of Acidic-Hydrolysis Residue Polysaccharides from Shiitake Culinary-Medicinal Mushroom Lentinus edodes (Agaricomycetes) in Mice.

Int J Med Mushrooms 2021 ;23(2):85-96

College of Life Sciences, Shandong Agricultural University, Tai'an 271018, People's Republic of China.

The present work was designed to investigate the antioxidant and hepatoprotective effects of acidic-hydrolysis Lentinus edodes residue polysaccharides (Ac-LRP) on lipopolysaccharide-induced liver injury in mice. Hepatoprotective effects were observed in liver treated with Ac-LRP at doses of 200, 400, and 600 mg/kg body weight, respectively. Activities of superoxide dismutase, glutathione peroxide, catalase, total antioxidant capacity, alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase and levels of total cholesterol, triglyceride, malondialdehyde, and lipid peroxidation in liver or serum samples were analyzed. Read More

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January 2021

Nanochitin whisker enhances insecticidal activity of chemical pesticide for pest insect control and toxicity.

J Nanobiotechnology 2021 Feb 16;19(1):49. Epub 2021 Feb 16.

Department of Entomology, College of Plant Protection, Henan Agricultural University, 450002, Zhengzhou, China.

Background: Nanomaterials in plant protection promise many benefits over conventional pesticide products. Nano-enabled pesticides may alter the functionality or risk profile of active ingredients. Cationic nanochitin whiskers (NC) possess strong biological activity against wheat aphids. Read More

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February 2021

Determination of vitamin E and its metabolites in equine urine using liquid chromatography-mass spectrometry.

Drug Test Anal 2021 Feb 1. Epub 2021 Feb 1.

K. L. Maddy Equine Analytical Chemistry Laboratory, California Animal Health and Food Safety Laboratory, School of Veterinary Medicine, University of California, Davis, California, USA.

Equine neuroaxonal dystrophy/degenerative myeloencephalopathy (eNAD/EDM) is a hereditary, deteriorating central nervous disease in horses. Currently, the only way to confirm eNAD/EDM is through a postmortem histological evaluation of the central nervous system. Vitamin E, specifically the isoform alpha-tocopherol (α-TP), is known to protect eNAD/EDM susceptible horses from developing the clinical phenotype. Read More

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February 2021

Hydrolytic Degradation of Heparin in Acidic Environments: Nuclear Magnetic Resonance Reveals Details of Selective Desulfation.

ACS Appl Mater Interfaces 2021 Feb 20;13(4):5551-5563. Epub 2021 Jan 20.

Biopolymer Research Centre, School of Applied Sciences, University of Huddersfield, Huddersfield HD1 3DH, United Kingdom.

Heparin is a complex glycosaminoglycan, derived mainly from pig mucosa, used therapeutically for its anticoagulant activity. Yet, owing largely to the chain complexity, the progressive effects of environmental conditions on heparin structure have not been fully described. A systematic study of the influence of acidic hydrolysis on heparin chain length and substitution has therefore been conducted. Read More

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February 2021

Ultrasensitive Quantification of Recombinant Proteins Using AAA-MS.

Methods Mol Biol 2019 ;2030:1-10

Univ. Grenoble Alpes, CEA, Inserm, U1038 BIG-BGE, Grenoble, France.

Recombinant proteins are essential components of therapeutic, biotechnological, food, and household products. In some cases, recombinant proteins must be purified and their quantity and/or concentration precisely determined. In this chapter, we describe a protocol for the quantification of purified recombinant proteins. Read More

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Implementation of the Sono-Heat-Exchanger in the Extra Virgin Olive Oil Extraction Process: End-User Validation and Analytical Evaluation.

Molecules 2019 Jun 27;24(13). Epub 2019 Jun 27.

Department of NEUROFARBA, and Multidisciplinary Centre of Research on Food Sciences (M.C.R.F.S.- Ce.R.A.), University of Florence, Via Ugo Schiff 6, 50019 Sesto F.no Firenze, Italy.

The use of innovative systems, such as the heat exchanger, for production of extra virgin olive oil should allow maintenance of the same quality of those oils derived from traditional processes, and presents specific advantages. The performance of this system was evaluated by (i) determining the parameters directly measurable by the olive millers (i.e. Read More

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and Anti-Salmonella Evaluation of Pectin Extracts and Hydrolysates from "Cas Mango" ().

Evid Based Complement Alternat Med 2019 23;2019:3578402. Epub 2019 Apr 23.

Medical Research and Applied Biochemistry Laboratory (Drug Discovery and Development Research Unit), University of Buea, Cameroon.

Background: The threat to human health posed by multidrug-resistant strains of S () and S () is of growing concern. Generally, there has been increasing resistance and even multidrug resistance to almost all classes of antibiotics. This has rendered treatment with antibiotics difficult and costly. Read More

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Anti-inflammatory and Cytotoxic Triterpenes from the Rot Roots of Panax notoginseng.

Nat Prod Bioprospect 2019 Aug 23;9(4):287-295. Epub 2019 May 23.

State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, People's Republic of China.

Four new protopanaxatriol-type triterpenes (1-2) and glucosides (3-4), were isolated from the rot roots of Panax notoginseng (Burk.) Chen, along with four known ones (5-8). Their structures were elucidated on the basis of extensive spectroscopic analysis (HRESIMS, NMR, UV, IR, and OR) and acidic hydrolysis. Read More

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The Effect of Molecular Weight on the Antibacterial Activity of ,,-Trimethyl Chitosan (TMC).

Int J Mol Sci 2019 Apr 9;20(7). Epub 2019 Apr 9.

Faculty of Pharmaceutical Sciences, School of Health Sciences, University of Iceland, Hofsvallagata 53, IS-107 Reykjavík, Iceland.

,,-trimethyl chitosan (TMC) with 93% degree of trimethylation was synthesized. TMC and the chitosan starting material were subjected to acidic hydrolysis to produce 49 different samples with a reduced average molecular weight (M) ranging from 2 to 144 kDa. This was done to allow the investigation of the relationship between antibacterial activity and M over a wide M range. Read More

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Study on a Fermented Whole Wheat: Phenolic Content, Activity on PTP1B Enzyme and In Vitro Prebiotic Properties.

Molecules 2019 Mar 21;24(6). Epub 2019 Mar 21.

Department of NEUROFARBA, and Multidisciplinary Centre of Research on Food Sciences (M.C.R.F.S.- Ce.R.A.), University of Florence, Via Ugo Schiff 6, 50019 Sesto F.no Firenze, Italy.

Fermented cereals, staple foods in Asia and Africa, are recently receiving a growing interest in Western countries. The object of this work is the characterization of a fermented wheat used as a food ingredient and dietary supplement. To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on and the microbial composition were investigated. Read More

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Triterpenoid saponins with hepatoprotective effects from the fresh leaves of .

Nat Prod Res 2020 May 16;34(10):1373-1379. Epub 2018 Nov 16.

State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, People's Republic of China.

The fresh leaves of (Araliaceae) have been used as a common wild vegetable for salad and soup, and also herbal tea by the local people living in its growing areas of Yunnan province, China. Detailed chemical investigation led to the identification of a new triterpenoid saponin, 3---L-arabinopyranosyl-28---D-glucopyranosyl-(1→6)---D-glucopyranosyl-3-hydroxyolean-12-ene-28,29-dioic acid () from the fresh leaves, together with 11 known compounds, including six triterpenoid saponins (-), two caffeoylquinic acid derivatives (-), and three flavonoid glycosides (-). Their structures were determined on the basis of spectroscopic analysis and acidic hydrolysis. Read More

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Synthesis and Self-Assembly of Xylan-Based Amphiphiles: From Bio-Based Vesicles to Antifungal Properties.

Biomacromolecules 2019 01 5;20(1):118-129. Epub 2018 Nov 5.

CNRS , LCPO, UMR 5629, 16 avenue Pey Berland , Pessac , F-33600 , France.

This work aims at designing functional biomaterials through selective chemical modification of xylan from beechwood. Acidic hydrolysis of xylan led to well-defined oligomers with an average of six xylose units per chain and with an aldehyde group at the reductive end. Reductive amination was performed on this aldehyde end group to introduce an azide reactive group. Read More

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January 2019

Antioxidative and hepatoprotective effects of enzymatic and acidic-hydrolysis of Pleurotus geesteranus mycelium polysaccharides on alcoholic liver diseases.

Carbohydr Polym 2018 Dec 15;201:75-86. Epub 2018 Aug 15.

College of Life Science, Shandong Agricultural University, Taian, 271018, PR China. Electronic address:

The present work investigated the antioxidant and hepatoprotective effects of acidic- and enzymatic-hydrolysis mycelium polysaccharide (AcMPS and EnMPS) from Pleurotus geesteranus on alcoholic liver disease (ALD) mice. The animal studies demonstrated that the polysaccharides had potential effects reflected by remitting alcoholic hepatitis, reducing lipid accumulation, preventing oxidative stress, improving inflammatory symptoms, and alleviating the liver functions by histopathologic observation. Results showed that AcMPS (yield of 84%) was composed of L-Rha, D-Rib, L-Ara, D-Glc, D-Man and D-Gal with the Mw of 3. Read More

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December 2018

Cashew apple bagasse as new feedstock for the hydrogen production using dark fermentation process.

J Biotechnol 2018 Nov 8;286:71-78. Epub 2018 Sep 8.

University of Naples Federico II, Department of Chemical, Materials and Production Engineering, P.le Tecchio, 80, 80125, Naples, Italy. Electronic address:

Cashew apple bagasse (CAB) has been studied as feedstock for the biohydrogen production using Clostridium roseum and the dark fermentation process. Pretreatment with alkaline hydrogen peroxide (CAB-AHP) on raw material and the acid and enzymatic hydrolysis have been taken into account to evaluate the H yields. Results show that the acid hydrolysate obtained from CAB produced higher H molar yield (HMY) (15 mmol/L) than the acid hydrolysate from CAB-AHP (4. Read More

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November 2018

Determination of N-(2-hydroxyethyl)valine in globin of ethylene oxide-exposed workers using total acidic hydrolysis and HPLC-ESI-MS.

Toxicol Lett 2018 Dec 21;298:76-80. Epub 2018 Jun 21.

Department of Organic Chemistry, Faculty of Chemical Technology, University of Chemistry and Technology, Technická 1905, 166 28 Prague, Czech Republic.

Ethylene oxide (EO), an industrial intermediate and gaseous sterilant for medical devices, is carcinogenic to humans, which warrants minimization of exposure in the workplaces. The principal analytical strategy currently used in biomonitoring of exposure to EO consists in the conversion of N-(2-hydroxyethyl) adduct at the N-terminal valine (HEV) in globin to a specific thiohydantoin derivative accessible to GC-MS analysis (modified Edman degradation, MED). Though highly sensitive, the method is laborious and, at least in our hands, not sufficiently robust. Read More

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December 2018

Chemo-Enzymatic Synthesis of Poly(4-piperidine lactone- b-ω-pentadecalactone) Block Copolymers as Biomaterials with Antibacterial Properties.

Biomacromolecules 2018 07 4;19(7):2673-2681. Epub 2018 May 4.

Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering , East China University of Science and Technology , Shanghai , 200237 , China.

With increasing troubles in bacterial contamination and antibiotic-resistance, new materials possessing both biocompatibility and antimicrobial efficacy are supposed to be developed for future biomedical application. Herein, we demonstrated a chemo-enzymatic ring opening polymerization (ROP) approach for block copolyester, that is, poly(4-benzyl formate piperidine lactone- b-ω-pentadecalactone) (PNPIL- b-PPDL), in a one-pot two-step process. Afterward, cationic poly(4-piperidine lactone- b-ω-pentadecalactone) (PPIL- b-PPDL) with pendent secondary amino groups was obtained via acidic hydrolysis of PNPIL- b-PPDL. Read More

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Antibacterial potential of the Cistus incanus L. phenolics as studied with use of thin-layer chromatography combined with direct bioautography and in situ hydrolysis.

J Chromatogr A 2018 Jan 23;1534:170-178. Epub 2017 Dec 23.

Institute of Chemistry, University of Silesia, 9 Szkolna Street, 40-006 Katowice, Poland.

The main aim of this study was to detect and identify antibacterial components of fraction I derived from eleven commercial C. incanus herbal teas. Fraction I obtained by a well-established phytochemical protocol of a multi-step extraction was expected to contain flavonoid aglycons alone. Read More

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January 2018

Degradation kinetics study of 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (PLAG) by a validated stability-indicating RP-HPLC method.

J Pharm Biomed Anal 2018 Feb 13;149:374-380. Epub 2017 Nov 13.

College of Pharmacy & Institute of Pharmaceutical Science and Technology, Hanyang University, 55 Hanyangdaehak-ro, Sangnok-gu, Ansan 426-791, Republic of Korea. Electronic address:

The chemical stability of 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (PLAG), a therapeutic agent for neutropenia, was investigated using a validated stability-indicating reversed phase high-performance liquid chromatographic (RP-HPLC) method. The forced degradation of PLAG was carried out under the stress conditions of hydrolysis (alkaline, acidic and various pH buffers), oxidation, photolysis and heat. A simple, sensitive, specific, robust, precise and accurate RP-HPLC method was developed and validated for evaluating the degradation kinetics of PLAG. Read More

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February 2018

Concept for Recycling Waste Biomass from the Sugar Industry for Chemical and Biotechnological Purposes.

Molecules 2017 Sep 13;22(9). Epub 2017 Sep 13.

Institute of General and Ecological Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, Poland.

The objective of this study was to develop a method for the thermally-assisted acidic hydrolysis of waste biomass from the sugar industry (sugar beet pulp and leaves) for chemical and biotechnological purposes. The distillates, containing furfural, can be catalytically reduced directly into furfurayl alcohol or tetrahydrofurfuryl alcohol. The sugars present in the hydrolysates can be converted by lactic bacteria into lactic acid, which, by catalytic reduction, leads to propylene glycol. Read More

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September 2017

Synthesis of MeON-neoglycosides of digoxigenin with 6-deoxy- and 2,6-dideoxy-d-glucose derivatives and their anticancer activity.

Bioorg Med Chem Lett 2017 08 3;27(15):3359-3364. Epub 2017 Jun 3.

Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, People's Republic of China; State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. Electronic address:

Cardiac glycosides show anticancer activities and their deoxy-sugar chains are vital for their anticancer effects. In order to study the structure-activity relationship (SAR) of cardiac glycosides toward cancers and get more potent anticancer agents, a series of MeON-neoglycosides of digoxigenin was synthesized and evaluated. First, ten 6-deoxy- and 2,6-dideoxy-d-glucopyranosyl donors were synthesized starting from methyl α-d-glucopyranoside and 2-deoxy-d-glucose. Read More

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Effects of Nanochitin on the Enhancement of the Grain Yield and Quality of Winter Wheat.

J Agric Food Chem 2018 Jul 24;66(26):6637-6645. Epub 2017 Jul 24.

This study investigated the effects of different rates of nanochitin in soil on the grain yield and quality of winter wheat. Nanochitin obtained by acidic hydrolysis of shrimp chitin was a rod-like whisker possessing a hydrodynamic diameter of 143 nm and ζ potential of 55.7 mV. Read More

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Cellulose nanocrystals from Actinidia deliciosa pruning residues combined with carvacrol in PVA_CH films with antioxidant/antimicrobial properties for packaging applications.

Int J Biol Macromol 2017 Nov 3;104(Pt A):43-55. Epub 2017 Jun 3.

Department of Agricultural and Forestry Science (DAFNE), University of Tuscia, Via S. Camillo De Lellis snc, 01100 Viterbo, Italy. Electronic address:

Kiwi Actinidia deliciosa pruning residues were here used for the first time as precursors for the extraction of high performing cellulose nanocrystals (CNC) by applying a bleaching treatment followed by an acidic hydrolysis. The resultant cellulosic nanostructures, obtained by an optimize extraction procedure (0.7% wt/v two times of sodium chlorite NaClO) followed by an hydrolysis step, were then used as reinforcements phases in poly(vinyl alcohol) (PVA) blended with natural chitosan (CH) based films and also combined, for the first time, with carvacrol used here as active agent. Read More

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November 2017

Surface-modified TiO nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro.

Colloids Surf B Biointerfaces 2017 Jul 14;155:323-331. Epub 2017 Apr 14.

Institute of Nuclear Sciences Vinča, University of Belgrade, P.O. Box 522, Serbia. Electronic address:

The antigenotoxic and antioxidative properties of surface-modified TiO nanoparticles (NPs) with ascorbic acid (AA) were compared with those of constituents (free AA and bare TiO NPs). Colloids consisting of the TiO NPs with anatase crystal structure were prepared by acidic hydrolysis of TiCl. The synthesized TiO NPs were characterized using transmission electron microscopy and X-ray diffraction analysis. Read More

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Metabolic profiling of tyrosine, tryptophan, and glutamate in human urine using gas chromatography-tandem mass spectrometry combined with single SPE cleanup.

J Chromatogr B Analyt Technol Biomed Life Sci 2017 Apr 16;1051:97-107. Epub 2017 Mar 16.

College of Pharmacy, Kyung Hee University, Seoul 130-701, Korea. Electronic address:

The tyrosine, tryptophan, and glutamate metabolic pathways play key roles on pathological state of neuronal functions and the change of their levels in biological systems reflects the progress degree of neuronal diseases. Comprehensive profiling of these metabolites is important to find new biomarkers for diagnosis or prognosis of various neuronal diseases. However, the overall profiling analysis of various neurochemicals in biological sample is confronted with several limitations due to their low concentration and physicochemical properties and the coexistence of matrices. Read More

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Protective effects on liver, kidney and pancreas of enzymatic- and acidic-hydrolysis of polysaccharides by spent mushroom compost (Hypsizigus marmoreus).

Sci Rep 2017 02 24;7:43212. Epub 2017 Feb 24.

College of Life Science, Shandong Agricultural University, Taian, 271018, PR China.

The present work investigated the protective effects on liver, kidneys and pancreas of spent mushroom compost polysaccharide (SCP) and its hydrolysates (enzymatic- (ESCP) and acid-hydrolyzed SCP (ASCP)) from Hypsizigus marmoreus, in streptozotocin (STZ)-induced diabetic mice. The results showed that enzymatic (superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT)) and non-enzymatic activities (total antioxidant capacity (T-AOC)) were significantly increased, the lipid peroxide contents (lipid peroxide (LPO) and malonaldehyde (MDA)) were remarkably reduced, and the clinical parameters were observably mitigated in diabetic mice treated with these three polysaccharides. Furthermore, histological observations also indicated recovery. Read More

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February 2017

Development and validation of HPLC and CE methods for simultaneous determination of amlodipine and atorvastatin in the presence of their acidic degradation products in tablets.

Acta Pharm 2016 Dec;66(4):479-490

Two methods were developed for separation and quantitation of amlodipine (AML) and atorvastatin (ATV) in the presence of their acidic degradation products. The first method was a simple isocratic RP-HPLC method while the second was capillary electrophoresis (CE). Degradation products were obtained by acidic hydrolysis of the two drugs and their structures were elucidated for the first time by IR and MS spectra. Read More

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December 2016

Biohydrogen and methane production via a two-step process using an acid pretreated native microalgae consortium.

Bioresour Technol 2016 Dec 13;221:324-330. Epub 2016 Sep 13.

Laboratory for Research on Advanced Processes for Water Treatment, Unidad Académica Juriquilla, Instituto de Ingeniería, Universidad Nacional Autónoma de México, Blvd. Juriquilla 3001, Querétaro 76230, Mexico. Electronic address:

A native microalgae consortium treated under thermal-acidic hydrolysis was used to produce hydrogen and methane in a two-step sequential process. Different acid concentrations were tested, generating hydrogen and methane yields of up to 45mLHgVS and 432mLCHgVS, respectively. The hydrogen production step solubilized the particulate COD (chemical oxygen demand) up to 30%, creating considerable amounts of volatile fatty acids (up to 10gCODL). Read More

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December 2016

pH-Triggered Sustained Drug Delivery from a Polymer Micelle having the β-Thiopropionate Linkage.

Macromol Rapid Commun 2016 Sep 22;37(18):1499-506. Epub 2016 Jul 22.

Polymer Science Unit, Indian Association for the Cultivation of Science, Jadavpur, Kolkata, 700032, India.

The synthesis, micellar aggregation, and pH-triggered intracellular drug delivery ability of an amphiphilic statistical copolymer (P2) are studied. Two methacrylate derivatives, one containing a hydrophilic pendant and the other containing a hydrophobic pendant chain, are copolymerized to produce P2. The hydrophobic pendant chain is linked to the polymer backbone by a β-thiopropionate linkage, known to undergo slow hydrolysis at mild acidic pH. Read More

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September 2016

Synthesis and Anti-HIV-1 Evaluation of Some Novel MC-1220 Analogs as Non-Nucleoside Reverse Transcriptase Inhibitors.

Arch Pharm (Weinheim) 2016 May 21;349(5):363-72. Epub 2016 Mar 21.

Dipartimento di Scienze e Tecnologie Biomediche, Sezione di Microbiologia e Virologia Generale e Biotecnologie Microbiche, Universita di Cagliari, Monserrato, Italy.

Some novel MC-1220 analogs were synthesized by condensation of 4,6-dichloro-N-methylpyrimidin-2-amine derivatives (1a,b and 15) and/or 4-chloro-6-methoxy-N,N,5-trimethylpyrimidin-2-amine (2a) with the sodium salt of 2,6-difluorophenylacetonitrile followed by treatment with aqueous sodium hydroxide in methanol, alkylation, reduction, halogenation, and/or acidic hydrolysis. All synthesized compounds were evaluated for their activity against HIV-1. The most active compound in this study was compound 7, which showed activity against HIV-1 comparable to that of MC-1220. Read More

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Hydrolytic Amino Acids Employed as a Novel Organic Nitrogen Source for the Preparation of PGPF-Containing Bio-Organic Fertilizer for Plant Growth Promotion and Characterization of Substance Transformation during BOF Production.

PLoS One 2016 14;11(3):e0149447. Epub 2016 Mar 14.

National Engineering Research Center for Organic-based Fertilizers, Nanjing Agricultural University, Nanjing, 210095, China.

Opportunity costs seriously limit the large-scale production of bio-organic fertilizers (BOFs) both in China and internationally. This study addresses the utilization of amino acids resulting from the acidic hydrolysis of pig corpses as organic nitrogen sources to increase the density of TrichodermaharzianumT-E5 (a typical plant growth-promoting fungi, PGPF). This results in a novel, economical, highly efficient and environmentally friendly BOF product. Read More

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