13,624 results match your criteria edta blood

Low level SARS-CoV-2 RNA detected in plasma samples from a cohort of Nigerians: Implications for blood transfusion.

PLoS One 2021 10;16(6):e0252611. Epub 2021 Jun 10.

Centre for Human Virology and Genomics, Microbiology Department, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria.

The present global pandemic triggered by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has lingered for over a year in its devastating effects. Diagnosis of coronavirus disease 2019 (COVID-19) is currently established with a polymerase chain reaction (PCR) test by means of oropharyngeal-, nasopharyngeal-, anal-swabs, sputum and blood plasma. However, oral and nasal swabs are more commonly used. Read More

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Application of SF-Cube 2.0 Technology in Platelet Count in Patients with EDTA-Dependent Pseudothrombocytopenia.

Clin Lab 2021 Jun;67(6)

Background: To explore the application of SF-Cube 2.0 technology in platelet count in patients with EDTA-dependent pseudothrombocytopenia (EDTA-PTCP).

Methods: Twenty-two out-patients and in-patients with EDTA-PTCP in our hospital were selected from February 1, 2020, to August 31, 2020. Read More

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Development of a method for the determination of the JAK2 gene mRNA in venous blood and assessment of its diagnostic value in oncohematology.

Klin Lab Diagn 2021 06;66(6):379-384

Krasnoyarsk branch of the «National Research Center for Hematology» Department of Health.

Overactive JAK pathway signaling is a hallmark of immune diseases and critically affects on inflammation and coagulation. A number of mutations in the JAK2 gene act as driving forces of myeloproliferative neoplasms (MPN), the pathogenesis of certain variants of acute leukemia, a number of solid malignancies and cardiovascular diseases. Assays for quantifying JAK2 mRNA in circulating blood cells can be used as a marker associated with the activity of this enzyme. Read More

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Folate Forms in RBC and Whole-Blood Lysates Appear Stable When Stored Frozen for 2 Years.

J Nutr 2021 Jun 5. Epub 2021 Jun 5.

Division of Laboratory Sciences, National Center for Environmental Health, CDC, Atlanta, GA, USA.

Background: The use of RBC lysate (RBC-Lys) eliminates the need for serum folate and hematocrit (Hct) measurement to calculate RBC folate. Information on the long-term frozen storage stability of RBC-Lys is missing.

Objectives: We aimed to assess the comparability of RBC folate forms in whole-blood lysate (WB-Lys) and RBC-Lys and the folate stability in both matrices. Read More

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Identification of leaky gut-related markers as indicators of metabolic health in Dutch adults: The Nutrition Questionnaires plus (NQplus) study.

PLoS One 2021 4;16(6):e0252936. Epub 2021 Jun 4.

Wageningen Food and Biobased Research, Wageningen University & Research, Wageningen, The Netherlands.

Background And Aim: Chronic inflammation is a primary risk factor for chronic metabolic disease and may be triggered by a "leaky gut." Several biomarkers have been recognized to indicate intestinal permeability (i.e. Read More

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Epidemic dropsy toxin, sanguinarine chloride, stimulates sucrose-sensitive hemolysis and breakdown of membrane phospholipid asymmetry in human erythrocytes.

Toxicon 2021 May 31;199:41-48. Epub 2021 May 31.

Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia.

Sanguinarine (SGN) is a benzophenathridine alkaloid extracted from Sanguinaria canadensis plant. SGN is incriminated in epidemic dropsy (ED) characterized by multiple-organ failure and anemia. Nevertheless, how SGN leads to anemia of ED remains poorly understood. Read More

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Coagulopathy and its associated factors among patients with a bleeding diathesis at the University of Gondar Specialized Referral Hospital, Northwest Ethiopia.

Thromb J 2021 Jun 1;19(1):36. Epub 2021 Jun 1.

Department of Hematology and Immunohematology, School of Medical Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, P.O. Box 196, Gondar, Ethiopia.

Background: Coagulopathy is the major cause of mortality and morbidity throughout the world. Globally, about 26-45% of healthy people have a history of bleeding symptoms, which may be a result of thrombocytopenia, factor deficiency, or pathological inhibitory.

Objective: To assess coagulopathy and its associated factors among patients with bleeding diathesis at the University of Gondar Specialized Referral Hospital from January to May 2020. Read More

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Prevalence of High HDL Cholesterol and Its Associated Factors Among Tunisian Women of Childbearing Age: A Cross-Sectional Study.

Int J Environ Res Public Health 2021 May 20;18(10). Epub 2021 May 20.

MoISA-Univ Montpellier, CIRAD, CIHEAM-IAMM, INRAE, Institut Agro, IRD, 911 Av. Agropolis, 34394 Montpellier, France.

The protective role of high high-density lipoprotein cholesterol (HDL-C) against cardiovascular risk has been questioned recently. Due to the increasing trend of cardiovascular diseases (CVD) in Tunisia, this study aimed to determine the prevalence of high HDL-C and its associated factors in Tunisian women of childbearing age. A cross-sectional survey was conducted among a subsample of 1689 women, aged 20 to 49 years, in the Great Tunis region. Read More

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Is of Significance in Young Dogs With Neurological Signs?

Front Vet Sci 2021 12;8:678968. Epub 2021 May 12.

Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands.

is a microsporidium belonging to the phylum Microspora. A few reports have described the clinical significance of infection in young dogs. In American and Japanese household dog populations, the seroprevalence was found to be 21%, indicating its wide-spread existence. Read More

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Association between 5'-UTR and 3'-UTR Polymorphisms of the TYMS Gene and Breast Cancer in Kurdish Women of Iraq.

Asian Pac J Cancer Prev 2021 May 1;22(5):1557-1560. Epub 2021 May 1.

Department of Medical Laboratory Sciences, College of Sciences, University of Raparin, Ranya, Kurdistan Region of Iraq.

Breast cancer is a common cancer found among women worldwide. Many polymorphisms can play a role in the development of this disease. The study was conducted to evaluate whether 2R/3R and 6bp insertion/deletion polymorphisms of the TYMS gene are associated with breast cancer risk in the Kurdish Iraqi population. Read More

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Significant association between Taq1 gene polymorphism in vitamin D receptor and chronic spontaneous urticaria in the Northeast of Iran.

Clin Mol Allergy 2021 May 27;19(1). Epub 2021 May 27.

Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

Objective: Chronic spontaneous urticaria (CSU) is defined as urticaria with an unknown etiology which persists for more than 6 weeks. CSU is an uncomfortable cutaneous condition that occurs due to an immune-mediated inflammatory reaction. Many studies have demonstrated that vitamin D deficiency and single-nucleotide polymorphisms in the vitamin D receptor (VDR) impact the immune response. Read More

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Characterization of Histopathology and Microbiota in Contemporary Regenerative Endodontic Procedures: Still Coming up Short.

J Endod 2021 May 24. Epub 2021 May 24.

Department of Conservative Dentistry, Dental Research Institute, Seoul National University Dental Hospital, Seoul National University School of Dentistry, Seoul, Republic of Korea; National Dental Care Center for Persons with Special Needs, Seoul National University Dental Hospital for Persons with Special Needs, Seoul, Republic of Korea. Electronic address:

Introduction: This study aimed to investigate microbiota and histopathology of infected immature teeth microenvironments following disinfection with calcium hydroxide, triple-antibiotic paste, and synthetic antimicrobial peptide (HBD3-C15) for regenerative endodontic procedures (REPs). The null hypothesis was that there is no difference among intracanal medications on disinfection in REPs.

Methods: Pulp necrosis and periapical lesions were induced in immature beagle dog premolars. Read More

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Designing, optimization, and validation of whole blood direct T-ARMS PCR for precise and rapid genotyping of complex vertebral malformation in cattle.

BMC Biotechnol 2021 May 22;21(1):36. Epub 2021 May 22.

Animal genetics & Breeding Division, ICAR-Central Institute for Research on Cattle, Meerut, UP, India.

Background: DNA testing in the cattle industry undergoes multiple hurdles. Successful genotyping involves the transportation of samples from the field to the laboratory in a chilled environment followed by DNA extraction, and finally, a specific genotyping protocol is followed. Various researches are focused on overcoming these issues. Read More

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Predictive Role of AURKA rs 1047972 Gene Polymorphism and the Risk of Development of Hepatocellular Carcinoma.

Immunol Invest 2021 May 21:1-11. Epub 2021 May 21.

Departement of Clinical Pathology, National Liver Institute, Menoufia University, Shebin El-Kom, Egypt.

Abbreviation: AFP: alpha-fetoprotein; ALP: alkaline phosphatase; ALT: alanine aminotransferase; AST: aspartate aminotransferase; AURKA: aurora kinase A; BCLC: Barcelona- Clinic Liver Cancer; CBC: complete blood count; CT: computed tomography; DM: diabetes mellitus; DNA: deoxyribonucleic acid; EDTA: ethylene diamine tetraacetic acid; GGT: gamma-glutamyl transferase; HB: hemoglobin; HBV: hepatitis B virus; HBsAg: hepatitis B surface antigen; HCC: hepatocellular carcinoma; HCV: hepatitis C virus; INR: international normalized ratio; mRNA: messenger ribonucleic acid; OR: odds ratio; PVT: portal vein thrombosis; RT-PCR: real-time polymerase chain reaction; SNP: single nucleotide polymorphism; WBCs: white blood cells. Read More

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Experimental evaluation of deep learning method in reticulocyte enumeration in peripheral blood.

Int J Lab Hematol 2021 May 20. Epub 2021 May 20.

Beijing Xiaoying Technology Co., Ltd, Beijing, China.

Introduction: Reticulocytes (RET) are immature red blood cells, and RET enumeration in peripheral blood has important clinical value in diagnosis, treatment efficacy observation, and prognosis of anemic diseases. For RET enumeration, flow cytometric reference method has shown to be more precise than the manual method by light microscopy. However, flow cytometric method generates occasionally spurious RET counts in some situations. Read More

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Quantification of ribociclib in dried blood spots by LC-MS/MS: Method development and clinical validation.

J Pharm Biomed Anal 2021 Jul 8;201:114118. Epub 2021 May 8.

Department of Medical Oncology, Erasmus MC Cancer Institute, Rotterdam, The Netherlands; Department of Hospital Pharmacy, Erasmus University Medical Center, Rotterdam, The Netherlands.

A reliable, specific, selective and robust liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the determination of ribociclib in both dried blood spot (DBS) samples and potassium EDTA plasma. DBS samples were obtained simultaneously with a plasma sample in advanced breast cancer patients treated with ribociclib. A 6 mm disk from the central part of the dried blood spot sample was punched, followed by extraction of ribociclib using liquid-liquid extraction spiked with ribociclib-d6 as internal standard. Read More

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SPINK1 Mutation in Idiopathic Chronic Pancreatitis: How Pertinent Is It in Coastal Eastern India?

Cureus 2021 Apr 12;13(4):e14427. Epub 2021 Apr 12.

Molecular Diagnostic and Research Center, Institute of Medical Sciences and SUM Hospital, Siksha 'O' Anusandhan Deemed to be University, Bhubaneswar, IND.

Background and aim Idiopathic chronic pancreatitis (ICP) is said to be present when no identifiable etiology can be identified. Robust evidence suggested that the serine protease inhibitor nucleus Kazol type 1 (SPINK1) N34S mutation was frequently associated with ICP. As there is a paucity of data on genetic studies in ICP cases from the coastal eastern region of India, we performed this study with an aim to evaluate the SPINK1 genetic mutations and other associated clinical correlates in ICP cases. Read More

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Mini-Factor H Modulates Complement-Dependent IL-6 and IL-10 Release in an Immune Cell Culture (PBMC) Model: Potential Benefits Against Cytokine Storm.

Front Immunol 2021 28;12:642860. Epub 2021 Apr 28.

Nanomedicine Research and Education Center, Institute of Translational Medicine, Semmelweis University, Budapest, Hungary.

Cytokine storm (CS), an excessive release of proinflammatory cytokines upon overactivation of the innate immune system, came recently to the focus of interest because of its role in the life-threatening consequences of certain immune therapies and viral diseases, including CAR-T cell therapy and Covid-19. Because complement activation with subsequent anaphylatoxin release is in the core of innate immune stimulation, studying the relationship between complement activation and cytokine release in an CS model holds promise to better understand CS and identify new therapies against it. We used peripheral blood mononuclear cells (PBMCs) cultured in the presence of autologous serum to test the impact of complement activation and inhibition on cytokine release, testing the effects of liposomal amphotericin B (AmBisome), zymosan and bacterial lipopolysaccharide (LPS) as immune activators and heat inactivation of serum, EDTA and mini-factor H (mfH) as complement inhibitors. Read More

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Does Hemodialysis Need to be Initiated to Improve Platelet Function in CKD G5 Patients? A Pilot Prospective, Observational Cohort Study.

Indian J Nephrol 2021 Jan-Feb;31(1):43-49. Epub 2020 Nov 11.

Department of Nephrology, Christian Medical College, Vellore, Tamil Nadu, India.

Introduction: We previously showed that patients with chronic kidney disease (CKD) Stage G4-5 have normal bleeding times. This made us question whether hemodialysis (HD) initiation was really necessary solely to improve platelet function.

Methods: In this prospective observational study, two 5 ml citrated blood samples and one 2 ml EDTA blood sample were collected from incident HD patients fulfilling inclusion criteria prior to HD initiation (baseline sample) and after three sessions of short duration, low flow, counter-current HD. Read More

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November 2020

HIV drug resistance among adults initiating antiretroviral therapy in Uganda.

J Antimicrob Chemother 2021 May 15. Epub 2021 May 15.

Uganda Virus Research Institute (UVRI), Entebbe, Uganda.

Background: WHO revised their HIV drug resistance (HIVDR) monitoring strategy in 2014, enabling countries to generate nationally representative HIVDR prevalence estimates from surveys conducted using this methodology. In 2016, we adopted this strategy in Uganda and conducted an HIVDR survey among adults initiating or reinitiating ART.

Methods: A cross-sectional survey of adults aged ≥18 years initiating or reinitiating ART was conducted at 23 sites using a two-stage cluster design sampling method. Read More

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Synthesis and evaluation of technetium-99m labelled 1-(2-methoxyphenyl)piperazine derivative for single photon emission computed tomography imaging for targeting 5-HT.

Bioorg Chem 2021 Jun 13;111:104972. Epub 2021 May 13.

Department of Chemistry, School of Physical & Decision Sciences (SPDS), Babasaheb Bhimrao Ambedkar University (A Central University), Lucknow 226025, UP, India; Division of Cyclotron and Radiopharmaceutical Sciences, Institute of Nuclear Medicine and Allied Sciences, Brig. S. K. Mazumdar Road, Delhi 110054, India. Electronic address:

Quantitative changes in expression level of 5HT are somewhere related to common neurological disorders such as anxiety, major depression and schizophrenia. We have designed EDTA conjugated SPECT imaging probe for localization of 5HT receptor in brain. For designing SPECT probe we have employed the concept of bivalent approach and a homodimeric system with desirable pharmacokinetics of 5HT imaging. Read More

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Diagnosis of knee prosthetic joint infection; aspiration and biopsy.

Knee 2021 May 5;30:249-253. Epub 2021 May 5.

Department of Microbiology, Royal Devon and Exeter Hospitals, Barrack Road, United Kingdom.

Background: Prosthetic joint infection (PJI) is a significant cause of morbidity and mortality following knee replacement surgery. The diagnosis can be challenging and is based on a combination of clinical suspicion, radiographic findings and also biochemical/ microbiological investigations. Our Aim was to review the role of aspiration and biopsy in the diagnosis of PJI in Total Knee Arthroplasty (TKA). Read More

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Inter-laboratory comparison of gene expression biodosimetry for protracted radiation exposures as part of the RENEB and EURADOS WG10 2019 exercise.

Sci Rep 2021 May 7;11(1):9756. Epub 2021 May 7.

Bundeswehr Institute of Radiobiology Affiliated to University Ulm, Neuherbergstr. 11, 80937, Munich, Germany.

Large-scale radiation emergency scenarios involving protracted low dose rate radiation exposure (e.g. a hidden radioactive source in a train) necessitate the development of high throughput methods for providing rapid individual dose estimates. Read More

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Flow cytometric analysis of platelet surface glycoproteins in the diagnosis of thirty-two Turkish patients with Glanzmann Thrombasthenia: a multicenter experience.

Turk J Med Sci 2021 May 7. Epub 2021 May 7.

Background/aim: Glanzmann thrombasthenia (GT) is a rare autosomal recessively inherited bleeding disorder characterized by the quantitative (type 1 and type 2) or qualitative (type 3) deficiency in platelet membrane glycoprotein (GP) IIb/IIIa (CD41a/CD61) fibrinogen receptors. In type 1, 2 and 3, CD41a/CD61 expression is 5%, 5-20% and above 20%, respectively. In this study, diagnosis of GT was confirmed and subgroups were identified in 32 Turkish patients by flow cytometry analysis. Read More

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White blood cells and platelet profiles of diabetic patients at University of Gondar specialized referral hospital: A comparative cross-sectional study.

J Clin Lab Anal 2021 Jun 3;35(6):e23808. Epub 2021 May 3.

Department of Hematology and Immunohematology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.

Background: Altered level of many hematological parameters such as white blood cells (WBC) and platelet function has been observed in diabetes mellitus (DM) patients. Therefore, this study aimed to determine the WBC and platelet profiles and their association with anthropometric measurement and blood pressure in DM patients and healthy controls.

Method: A comparative cross-sectional study was conducted on a total of 246 participants at the University of Gondar Specialized Referral Hospital. Read More

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Order of draw of blood samples affect potassium results without K-EDTA contamination during routine workflow.

Biochem Med (Zagreb) 2021 Jun 15;31(2):020704. Epub 2021 Apr 15.

Department of Internal Medicine, Lüleburgaz State Hospital, Kırklareli, Turkey.

Introduction: A specific sequence is recommended for filling blood tubes during blood collection to prevent erroneous test results due to carryover of additives. However, requirement of this procedure is still debatable. This study was aimed to investigate the potassium ethylenediaminetetraacetic acid (K-EDTA) contamination in blood samples taken after a tube containing the additive during routine workflow. Read More

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Putative Novel Atypical BTV Serotype '36' Identified in Small Ruminants in Switzerland.

Viruses 2021 04 21;13(5). Epub 2021 Apr 21.

Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald-Insel Riems, Germany.

We identified a putative novel atypical BTV serotype '36' in Swiss goat flocks. In the initial flock clinical signs consisting of multifocal purulent dermatitis, facial oedema and fever were observed. Following BTV detection by RT-qPCR, serotyping identified BTV-25 and also a putative novel BTV serotype in several of the affected goats. Read More

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Triple-Drug Treatment Is Effective for Lymphatic Filariasis Microfilaria Clearance in Samoa.

Trop Med Infect Dis 2021 Apr 1;6(2). Epub 2021 Apr 1.

School of Public Health, The University of Queensland, Brisbane, QLD 4006, Australia.

Following the first triple-drug mass drug administration (MDA) for lymphatic filariasis in Samoa in 2018, unexpected persistence of microfilaria (Mf) positivity in 18 (15%) of 121 antigen-positive persons was observed in a nationwide household survey 1-2 months later. Of the 18 Mf positive persons, 14 reported taking the MDA, raising concerns about MDA efficacy. In 2019, 5-6 months after the 2018 survey, a monitored treatment study was done to evaluate directly observed weight-based treatment in these Mf positive individuals. Read More

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Simultaneous detection and differentiation of clinically relevant relapsing fever with semi-multiplex real-time PCR.

J Clin Microbiol 2021 Apr 28. Epub 2021 Apr 28.

Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA.

Bacterial vector-borne diseases, including species, present a significant diagnostic, clinical, and public health challenge due to their overlapping symptoms and the breadth of causative agents and arthropod vectors. The relapsing fever (RF) borreliae encompass both established and emerging pathogens and are transmitted to humans by soft ticks, hard ticks, or lice. We developed a real-time semi-multiplex PCR assay that detects multiple RF borreliae causing human illness and classifies them into one of three groups. Read More

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Follow-up of dairy cattle naturally infected by Trypanosoma vivax after treatment with isometamidium chloride.

Rev Bras Parasitol Vet 2021 26;30(1):e020220. Epub 2021 Apr 26.

Faculdade de Ciências Agrárias e Veterinárias - FCAV, Universidade Estadual Paulista - UNESP, Jaboticabal, SP, Brasil.

Trypanosoma vivax infections cause nonspecific clinical signs in cattle associated with aparasitemic intervals, making disease diagnosis a challenge. In Brazil, diminazene aceturate and isometamidium chloride (ISM) are available to treat bovine trypanosomosis. The objective of this study was to follow-up, by molecular and serological techniques, dairy cattle naturally infected by T. Read More

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