30,984 results match your criteria ectopic expression

Ectopic expression of GhIQD14 (cotton IQ67 domain-containing protein 14) causes twisted organ and modulates secondary wall formation in Arabidopsis.

Plant Physiol Biochem 2021 Apr 5;163:276-284. Epub 2021 Apr 5.

Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou, China; Key Laboratory of Biotechnology and Crop Quality Improvement of Ministry of Agriculture, Biotechnology Research Center, Southwest University, Chongqing, China. Electronic address:

In plants, although KNOX genes are known to regulate secondary cell wall (SCW) formation, their protein-regulating mechanisms remain largely unknown. Here, we showed that GhKNL1, which regulates SCW formation and fiber development in cotton, could interact with an IQ67 domain containing protein (GhIQD14) in yeast. Confocal observation showed that GhIQD14 was localized to the microtubules. Read More

View Article and Full-Text PDF

Analysis of differences in the transcriptomic profiles of eutopic and ectopic endometriums in women with ovarian endometriosis.

PeerJ 2021 7;9:e11045. Epub 2021 Apr 7.

Department of Obstetrics and Gynecology, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.

Background: Endometriosis is a common gynecological disease among women in their reproductive years. Although much effort has been made, the pathogenesis of this disease and the detailed differences between eutopic endometrial cells and ectopic endometrial cells are still unclear.

Methods: In this study, eutopic and ectopic endometrial cells were collected from patients with and without endometriosis and RNA sequencing was performed. Read More

View Article and Full-Text PDF

MAFG-driven osteosarcoma cell progression is inhibited by a novel miRNA miR-4660.

Mol Ther Nucleic Acids 2021 Jun 13;24:385-402. Epub 2021 Mar 13.

Jiangsu Key Laboratory of Neuropsychiatric Diseases and Institute of Neuroscience, Soochow University, Suzhou 215123, China.

Osteosarcoma (OS) is the most common primary bone malignancy in the adolescent population. MAFG (v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog G) forms a heterodimer with Nrf2 (NF-E2-related factor 2), binding to antioxidant response element (ARE), which is required for Nrf2 signaling activation. We found that mRNA and protein expression is significantly elevated in human OS tissues as well as in established and primary human OS cells. Read More

View Article and Full-Text PDF

ΔNp63 is a pioneer factor that binds inaccessible chromatin and elicits chromatin remodeling.

Epigenetics Chromatin 2021 Apr 17;14(1):20. Epub 2021 Apr 17.

Department of Biochemistry, State University of New York at Buffalo, Buffalo, NY, 14203, USA.

Background: ΔNp63 is a master transcriptional regulator playing critical roles in epidermal development and other cellular processes. Recent studies suggest that ΔNp63 functions as a pioneer factor that can target its binding sites within inaccessible chromatin and induce chromatin remodeling.

Methods: In order to examine if ΔNp63 can bind to inaccessible chromatin and to determine if specific histone modifications are required for binding, we induced ΔNp63 expression in two p63-naïve cell lines. Read More

View Article and Full-Text PDF

Homozygous missense mutation in UQCRC2 associated with severe encephalomyopathy, mitochondrial complex III assembly defect and activation of mitochondrial protein quality control.

Biochim Biophys Acta Mol Basis Dis 2021 Apr 15:166147. Epub 2021 Apr 15.

Department of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic. Electronic address:

The mitochondrial respiratory chain (MRC) complex III (CIII) associates with complexes I and IV (CI and CIV) into supercomplexes. We identified a novel homozygous missense mutation (c.665G>C; p. Read More

View Article and Full-Text PDF

Dysfunction of the Klotho-miR-30s/TRPC6 axis confers podocyte injury.

Biochem Biophys Res Commun 2021 Apr 13;557:90-96. Epub 2021 Apr 13.

Department of Nephrology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.

Klotho deficiency was observed in virtually all kinds of kidney disease and is thought to play a critical role in podocyte injury. However, the underline mechanisms involved in podocyte injury remain unknown. miRNAs have diverse regulatory roles, and miR-30 family members were essential for podocyte homeostasis. Read More

View Article and Full-Text PDF

Tumor suppressor DRD2 facilitates M1 macrophages and restricts NF-κB signaling to trigger pyroptosis in breast cancer.

Theranostics 2021 5;11(11):5214-5231. Epub 2021 Mar 5.

Key Laboratory of Molecular Oncology and Epigenetics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Breast cancer (BrCa) is the most common cancer worldwide, and the 5-year relative survival rate has declined in patients diagnosed at stage IV. Advanced BrCa is considered as incurable, which still lack effective treatment strategies. Identifying and characterizing new tumor suppression genes is important to establish effective prognostic biomarkers or therapeutic targets for late-stage BrCa. Read More

View Article and Full-Text PDF

Genome-wide specificity of prime editors in plants.

Nat Biotechnol 2021 Apr 15. Epub 2021 Apr 15.

State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.

Although prime editors (PEs) have the potential to facilitate precise genome editing in therapeutic, agricultural and research applications, their specificity has not been comprehensively evaluated. To provide a systematic assessment in plants, we first examined the mismatch tolerance of PEs in plant cells and found that the editing frequency was influenced by the number and location of mismatches in the primer binding site and spacer of the prime editing guide RNA (pegRNA). Assessing the activity of 12 pegRNAs at 179 predicted off-target sites, we detected only low frequencies of off-target edits (0. Read More

View Article and Full-Text PDF

Long non-coding RNA LEISA promotes progression of lung adenocarcinoma via enhancing interaction between STAT3 and IL-6 promoter.

Oncogene 2021 Apr 15. Epub 2021 Apr 15.

Department of Endocrinology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.

Long non-coding RNAs (lncRNAs) are emerging as a new class of regulators for a variety of biological processes and have been suggested to play pivotal roles in cancer development and progression. Our current study found that a lncRNA, designated enhancing IL-6/STAT3 signaling activation (LEISA, ENST00000603468), functioned as an oncogenic lncRNA in lung adenocarcinoma (LAD), a major form of non-small cell lung carcinoma, which is one of the most frequently diagnosed malignancies with high morbidity and mortality worldwide, and was involved in the regulation of STAT3 induced IL-6 transcription. Our data showed that LEISA was highly expressed in, and correlated with the clinical progression and prognosis of LAD. Read More

View Article and Full-Text PDF

Selenoprotein W ensures physiological bone remodeling by preventing hyperactivity of osteoclasts.

Nat Commun 2021 04 15;12(1):2258. Epub 2021 Apr 15.

Department of Microbiology, Laboratory of Bone Metabolism and Control, Yeungnam University College of Medicine, Daegu, Korea.

Selenoproteins containing selenium in the form of selenocysteine are critical for bone remodeling. However, their underlying mechanism of action is not fully understood. Herein, we report the identification of selenoprotein W (SELENOW) through large-scale mRNA profiling of receptor activator of nuclear factor (NF)-κΒ ligand (RANKL)-induced osteoclast differentiation, as a protein that is downregulated via RANKL/RANK/tumour necrosis factor receptor-associated factor 6/p38 signaling. Read More

View Article and Full-Text PDF

FCN3 functions as a tumor suppressor of lung adenocarcinoma through induction of endoplasmic reticulum stress.

Cell Death Dis 2021 Apr 15;12(4):407. Epub 2021 Apr 15.

Department of Life Science, Ewha Womans University, Seoul, Republic of Korea.

In this study, we report a novel function of FCN3 (Ficolin 3), a secreted lectin capable of activating the complement pathway, as a tumor suppressor of lung adenocarcinoma (LUAD). First, the expression of FCN3 was strongly down-regulated in cancer tissues compared to matched normal lung tissues, and down-regulation of FCN3 was shown to be significantly correlated with increased mortality among LUAD patients. Interestingly, while ectopic expression of FCN3 led to cell cycle arrest and apoptosis in A549 and H23 cells derived from LUAD, the secreted form of the protein had no effect on the cells. Read More

View Article and Full-Text PDF

Circular RNA hsa_circ_0003288 induces EMT and invasion by regulating hsa_circ_0003288/miR-145/PD-L1 axis in hepatocellular carcinoma.

Cancer Cell Int 2021 Apr 15;21(1):212. Epub 2021 Apr 15.

Department of Interventional Radiology, First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, Jiangsu, 215006, China.

Background: Epithelial-mesenchymal transition (EMT) has been associated with wound healing, tumorigenesis, and metastasis. Circular RNAs (circRNAs) are functional non-coding RNAs involved in multiple human cancers. However, whether and how circRNAs contribute to the EMT in hepatocellular carcinomas (HCC) remains to be deciphered. Read More

View Article and Full-Text PDF

PMEPA1 stimulates the proliferation, colony formation of pancreatic cancer cells via the MAPK signaling pathway.

Am J Med Sci 2021 Apr 12. Epub 2021 Apr 12.

Department of Hepatobiliary and Pancreatic Surgery, the Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, 266003, China. Electronic address:

Background: Prostate transmembrane protein androgen-induced 1 (PMEPA1) is reportedly highly expressed in pancreatic cancer (PC). However, its biological role and associated mechanisms have not been addressed in PC progression.

Methods: PMEPA1 mRNA expression and survival outcome of PC patients were evaluated via the GEPIA website. Read More

View Article and Full-Text PDF

A family of pathogen-induced cysteine-rich transmembrane proteins is involved in plant disease resistance.

Planta 2021 Apr 15;253(5):102. Epub 2021 Apr 15.

Plant-Microbe Interactions, Department of Biology, Science4Life, Utrecht University, 800.56, 3508 TB, Utrecht, The Netherlands.

Main Conclusion: Overexpression of pathogen-induced cysteine-rich transmembrane proteins (PCMs) in Arabidopsis thaliana enhances resistance against biotrophic pathogens and stimulates hypocotyl growth, suggesting a potential role for PCMs in connecting both biological processes. Plants possess a sophisticated immune system to protect themselves against pathogen attack. The defense hormone salicylic acid (SA) is an important player in the plant immune gene regulatory network. Read More

View Article and Full-Text PDF

5-Fluorouracil blocks quorum-sensing of biofilm-embedded methicillin-resistant Staphylococcus aureus in mice.

Nucleic Acids Res 2021 Apr 15. Epub 2021 Apr 15.

Department of Biosystems Science and Engineering, ETH Zürich, Mattenstrasse 26, CH-4058 Basel, Switzerland.

Antibiotic-resistant pathogens often escape antimicrobial treatment by forming protective biofilms in response to quorum-sensing communication via diffusible autoinducers. Biofilm formation by the nosocomial pathogen methicillin-resistant Staphylococcus aureus (MRSA) is triggered by the quorum-sensor autoinducer-2 (AI-2), whose biosynthesis is mediated by methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) and S-ribosylhomocysteine lyase (LuxS). Here, we present a high-throughput screening platform for small-molecular inhibitors of either enzyme. Read More

View Article and Full-Text PDF

Ectopic expression of CGG-repeats alters ovarian response to gonadotropins and leads to infertility in a murine FMR1 premutation model.

Hum Mol Genet 2021 Apr 15. Epub 2021 Apr 15.

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030.

Women heterozygous for an expansion of CGG repeats in the 5'UTR of FMR1 risk developing Fragile X-associated Primary Ovarian Insufficiency (FXPOI) and/or Tremor and Ataxia Syndrome (FXTAS). We show that expanded CGGs, independent of FMR1, are sufficient to drive ovarian insufficiency and that expression of CGG-containing mRNAs alone or in conjunction with a polyglycine-containing peptide translated from these RNAs contribute to dysfunction. Heterozygous females from two mouse lines expressing either CGG RNA-only (RNA-only) or CGG RNA and the polyglycine product FMRpolyG (FMRpolyG+RNA) were used to assess ovarian function in aging animals. Read More

View Article and Full-Text PDF

MiR-450a-5p Inhibits Gastric Cancer Cell Proliferation, Migration, and Invasion and Promotes Apoptosis Targeting CREB1 and Inhibiting AKT/GSK-3β Signaling Pathway.

Front Oncol 2021 29;11:633366. Epub 2021 Mar 29.

Department of General Surgery, The First Affiliated Hospital of University of Science and Technology of China, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.

Gastric cancer seriously affects human health and research on gastric cancer is attracting more and more attentions. In recent years, molecular targets have become the research focus. Accumulating evidence indicates that miR-450a-5p plays a critical role in cancer progression. Read More

View Article and Full-Text PDF

MiR-573 suppresses cell proliferation, migration and invasion via regulation of E2F3 in pancreatic cancer.

J Cancer 2021 19;12(10):3033-3044. Epub 2021 Mar 19.

Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.

Pancreatic cancer is among the most lethal malignancies worldwide. In this study, we aimed to determine whether miR-573 could suppress pancreatic cancer cell proliferation, migration, and invasion by targeting E2F3. MiR-573 expression in pancreatic cancer tissues and cell lines was measured using real-time PCR. Read More

View Article and Full-Text PDF

Orphan nuclear receptor ERR-γ regulates hepatic FGF23 production in acute kidney injury.

Proc Natl Acad Sci U S A 2021 Apr;118(16)

School of Biological Sciences and Technology, Chonnam National University, 61186 Gwangju, Republic of Korea;

Fibroblast growth factor 23 (FGF23), a hormone generally derived from bone, is important in phosphate and vitamin D homeostasis. In acute kidney injury (AKI) patients, high-circulating FGF23 levels are associated with disease progression and mortality. However, the organ and cell type of FGF23 production in AKI and the molecular mechanism of its excessive production are still unidentified. Read More

View Article and Full-Text PDF

CircPTPRA blocks the recognition of RNA N-methyladenosine through interacting with IGF2BP1 to suppress bladder cancer progression.

Mol Cancer 2021 Apr 14;20(1):68. Epub 2021 Apr 14.

Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Background: Circular RNAs (circRNAs) have been found to have significant impacts on bladder cancer (BC) progression through various mechanisms. In this study, we aimed to identify novel circRNAs that regulate the function of IGF2BP1, a key mA reader, and explore the regulatory mechanisms and clinical significances in BC.

Methods: Firstly, the clinical role of IGF2BP1 in BC was studied. Read More

View Article and Full-Text PDF

Gasdermin E permits interleukin-1 beta release in distinct sublytic and pyroptotic phases.

Cell Rep 2021 Apr;35(2):108998

Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA. Electronic address:

Cellular inflammasome activation causes caspase-1 cleavage of the pore-forming protein gasdermin D (GSDMD) with subsequent pyroptotic cell death and cytokine release. Here, we clarify the ambiguous role of the related family member gasdermin E (GSDME) in this process. Inflammasome stimulation in GSDMD-deficient cells led to apoptotic caspase cleavage of GSDME. Read More

View Article and Full-Text PDF

Generation of a new mouse line with conditionally activated signaling through the BMP receptor, ACVR1: A tool to characterize pleiotropic roles of BMP functions.

Genesis 2021 Apr 14:e23419. Epub 2021 Apr 14.

Department of Biologic and Materials Sciences & Prosthodontics, School of Dentistry, University of Michigan, MI, USA.

BMP signaling plays pleiotropic roles in various tissues during embryogenesis and after birth. We have previously generated a constitutively activated Acvr1(ca-Acvr1) transgenic mouse line (line L35) through pronuclei injection to investigate impacts of enhanced BMP signaling in a tissue specific manner. However, line L35 shows a restricted expression pattern of the transgene. Read More

View Article and Full-Text PDF

USP18-deficiency in cervical carcinoma is crucial for the malignant behavior of tumor cells in an ERK signal-dependent manner.

Oncol Lett 2021 May 29;21(5):421. Epub 2021 Mar 29.

Department of Human Anatomy, Basic Medical College, Jiamusi University, Jiamusi, Heilongjiang 154007, P.R. China.

Ubiquitin-specific peptidase (USP)18 belongs to the USP family, and is involved in cleaving and removing ubiquitin or ubiquitin-like molecules from their target molecules. Recently, increasing evidence has suggested that USP18 is constitutively expressed in different types of human tumors, and ectopic expression or downregulation of USP18 expression may contribute to tumorigenesis. However, the role of USP18 in uterine cervical cancer (UCC) remains unclear. Read More

View Article and Full-Text PDF

3D reconstruction and histopathological analyses on murine corporal body.

Reprod Med Biol 2021 Apr 9;20(2):199-207. Epub 2021 Feb 9.

Department of Developmental Genetics Institute of Advanced Medicine Wakayama Medical University Wakayama Japan.

Purpose: Erectile dysfunction (ED) is one of the increasing diseases with aging society. The basis of ED derived from local penile abnormality is poorly understood because of the complex three-dimensional (3D) distribution of sinusoids in corpus cavernosum (CC). Understanding the 3D histological structure of penis is thus necessary. Read More

View Article and Full-Text PDF

Wnt7a promotes the osteogenic differentiation of human mesenchymal stem cells.

Int J Mol Med 2021 Jun 13;47(6). Epub 2021 Apr 13.

Department of Spinal Surgery, Orthopaedic Medical Center, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, P.R. China.

Mesenchymal stem cells (MSCs) have the ability of differentiating into osteoblasts. Elucidating the molecular mechanisms of MSC differentiation into osteoblasts may provide novel therapeutic strategies for bone‑related diseases. Increasing evidence has confirmed that Wnt signaling plays the key role in osteoblast differentiation; however, the role of individual Wnt proteins in osteogenesis needs to be investigated. Read More

View Article and Full-Text PDF

An efficient vector-based CRISPR/Cas9 system in an Oreochromis mossambicus cell line using endogenous promoters.

Sci Rep 2021 Apr 12;11(1):7854. Epub 2021 Apr 12.

Biochemical Evolution Laboratory, Department of Animal Science, University of California, Davis, CA, 95616, USA.

CRISPR/Cas9 gene editing is effective in manipulating genetic loci in mammalian cell cultures and whole fish but efficient platforms applicable to fish cell lines are currently limited. Our initial attempts to employ this technology in fish cell lines using heterologous promoters or a ribonucleoprotein approach failed to indicate genomic alteration at targeted sites in a tilapia brain cell line (OmB). For potential use in a DNA vector approach, endogenous tilapia beta Actin (OmBAct), EF1 alpha (OmEF1a), and U6 (TU6) promoters were isolated. Read More

View Article and Full-Text PDF

RUNX2 promotes vascular injury repair by activating miR-23a and inhibiting TGFBR2.

Ann Transl Med 2021 Mar;9(5):363

Department of General & Vascular Surgery, Xiangya Hospital, Central South University, Changsha, China.

Background: Previous evidence has suggested that the transcription factor, runt-related transcription factor 2 (RUNX2), promotes the repair of vascular injury and activates the expression of microRNA-23a (miR-23a). TGF-β receptor type II (TGFBR2) has been found to mediate smooth muscle cells (SMCs) following arterial injury. However, the interactions among RUNX2, miR-23a and TGFBR2 in vascular injury have not been investigated thoroughly yet. Read More

View Article and Full-Text PDF

MICAL2 Facilitates Gastric Cancer Cell Migration via MRTF-A-Mediated CDC42 Activation.

Front Mol Biosci 2021 24;8:568868. Epub 2021 Mar 24.

Department of Physiology, Nanjing Medical University, Nanjing, China.

Cell migration is driven by the reorganization of the actin cytoskeleton. Although MICAL2 is known to mediate the oxidation of actin filaments to regulate F-actin dynamics, relatively few studies have investigated the potential role of MICAL2 during cancer cell migration. The migratory ability of gastric cancer cells was measured by wound healing and transwell assays. Read More

View Article and Full-Text PDF

High mobility group box-1 promotes inflammation in endometriotic stromal cells through Toll-like receptor 4/nuclear factor-kappa B.

Am J Transl Res 2021 15;13(3):1400-1410. Epub 2021 Mar 15.

Department of Obstetrics and Gynecology, Severance Hospital, Yonsei University College of Medicine Seoul, Republic of Korea.

Objective: To investigate whether high-mobility group box-1 induces cell proliferation, invasion and mediates inflammation in ectopic human endometrial stromal cells through Toll-like receptor 4.

Methods: Ectopic endometrial specimens were retrieved from patients with ovarian endometrioma having laparoscopy. Ectopic HESCs were treated with HO and recombinant HMGB-1 to induce oxidative stress. Read More

View Article and Full-Text PDF

Conversion of Reactive Astrocytes to Induced Neurons Enhances Neuronal Repair and Functional Recovery After Ischemic Stroke.

Front Aging Neurosci 2021 26;13:612856. Epub 2021 Mar 26.

Department of Anesthesiology, Emory University School of Medicine, Atlanta, GA, United States.

The master neuronal transcription factor NeuroD1 can directly reprogram astrocytes into induced neurons (iNeurons) after stroke. Using viral vectors to drive ectopic ND1 expression in gliotic astrocytes after brain injury presents an autologous form of cell therapy for neurodegenerative disease. Cultured astrocytes transfected with ND1 exhibited reduced proliferation and adopted neuronal morphology within 2-3 weeks later, expressed neuronal/synaptic markers, and extended processes. Read More

View Article and Full-Text PDF