5,813 results match your criteria e1a

Using the E1A Minigene Tool to Study mRNA Splicing Changes.

J Vis Exp 2021 Apr 22(170). Epub 2021 Apr 22.

Faculdade de Ciências Farmacêuticas, Universidade Estadual de Campinas;

mRNA processing involves multiple simultaneous steps to prepare mRNA for translation, such as 5´capping, poly-A addition and splicing. Besides constitutive splicing, alternative mRNA splicing allows the expression of multifunctional proteins from one gene. As interactome studies are generally the first analysis for new or unknown proteins, the association of the bait protein with splicing factors is an indication that it can participate in mRNA splicing process, but to determine in what context or what genes are regulated is an empirical process. Read More

View Article and Full-Text PDF

A Chemo-Genomic Approach Identifies Diverse Epigenetic Therapeutic Vulnerabilities in MYCN-Amplified Neuroblastoma.

Front Cell Dev Biol 2021 21;9:612518. Epub 2021 Apr 21.

Systems Biology Ireland and Precision Oncology Ireland, School of Medicine, University College Dublin, Dublin, Ireland.

Although a rare disease, neuroblastoma accounts for the highest proportion of childhood cancer deaths. There is a lack of recurrent somatic mutations in neuroblastoma embryonal tumours, suggesting a possible role for epigenetic alterations in driving this cancer. While an increasing number of reports suggest an association of MYCN with epigenetic machinery, the mechanisms of these interactions are poorly understood in the neuroblastoma setting. Read More

View Article and Full-Text PDF

CREG1 promotes lysosomal biogenesis and function.

Autophagy 2021 May 8:1-17. Epub 2021 May 8.

Department of Surgery, Rutgers University-Robert Wood Johnson Medical School, New Brunswick, NJ, USA.

CREG1 is a small glycoprotein which has been proposed as a transcription repressor, a secretory ligand, a lysosomal, or a mitochondrial protein. This is largely because of lack of antibodies for immunolocalization validated through gain- and loss-of-function studies. In the present study, we demonstrate, using antibodies validated for immunofluorescence microscopy, that CREG1 is mainly localized to the endosomal-lysosomal compartment. Read More

View Article and Full-Text PDF

New perspectives on the evolution of within-individual genome variation and germline/soma distinction.

Genome Biol Evol 2021 May 8. Epub 2021 May 8.

Département de Biologie, Université de Moncton, Moncton, NB, E1A 3E9, Canada.

Genomes can vary significantly even within the same individual. The underlying mechanisms are manifold, ranging from somatic mutation and recombination, development-associated ploidy changes and genetic bottlenecks, over to programmed DNA elimination during germline/soma differentiation. In this perspective piece, we briefly review recent developments in the study of within-individual genome variation in eukaryotes and prokaryotes. Read More

View Article and Full-Text PDF

Toward an Understanding of the Structural and Mechanistic Aspects of Protein-Protein Interactions in 2-Oxoacid Dehydrogenase Complexes.

Life (Basel) 2021 Apr 29;11(5). Epub 2021 Apr 29.

Department of Chemistry, Rutgers, The State University of New Jersey, Newark, NJ 07102, USA.

The 2-oxoglutarate dehydrogenase complex (OGDHc) is a key enzyme in the tricarboxylic acid (TCA) cycle and represents one of the major regulators of mitochondrial metabolism through NADH and reactive oxygen species levels. The OGDHc impacts cell metabolic and cell signaling pathways through the coupling of 2-oxoglutarate metabolism to gene transcription related to tumor cell proliferation and aging. is a gene encoding 2-oxoadipate dehydrogenase (E1a), which functions in the L-lysine degradation pathway. Read More

View Article and Full-Text PDF

Meta-Analysis and Systematic Review of Micro- and Macro-Nutrient Intakes and Trajectories of Macro-Nutrient Supply in the Eastern Mediterranean Region.

Nutrients 2021 Apr 30;13(5). Epub 2021 Apr 30.

Regional Office for the Eastern Mediterranean (EMRO), World Health Organization (WHO), Cairo 11371, Egypt.

The Eastern Mediterranean Region (EMR) is experiencing a nutrition transition, characterized by the emergence of overnutrition and micro-nutrient deficiencies. No previous study has comparatively examined nutrient intake in adults across countries in the EMR. This review examined the adequacy of nutrients in adults living in the EMR. Read More

View Article and Full-Text PDF

Trajectories of Eating Behaviour Changes during Adolescence.

Nutrients 2021 Apr 16;13(4). Epub 2021 Apr 16.

Centre de Formation Médicale du Nouveau-Brunswick (Université de Sherbrooke), Pavillon J.-Raymond-Frenette, Université de Moncton, 18 Antonine-Maillet Ave, Moncton, NB E1A 3E9, Canada.

Adolescence represents a critical transition phase during which individuals acquire eating behaviours that can track into adulthood. This study aims to characterise trends in eating behaviours throughout adolescence by investigating the presence of sub-groups of individuals presenting distinct trajectories of vegetable and fruit, sugary beverage, breakfast and fast-food consumption. Data from 744 MATCH study Canadian participants followed from 11 to 18 Years old (2013-2019) were included in the analyses. Read More

View Article and Full-Text PDF

Effects of monochloramine on culturability, viability and persistence of Pseudomonas putida and tap water mixed bacterial community.

Appl Microbiol Biotechnol 2021 May 22;105(9):3799-3810. Epub 2021 Apr 22.

Department of Civil and Environmental Engineering, National University of Singapore, 1 Engineering Drive 2, #07-03 E1A, Singapore, 117576, Singapore.

Bacterial biofilms are able to persist in drinking water distribution systems (DWDS) even if disinfectants such as monochloramine are used to inhibit bacterial colonization and biofilm formation. While studies have determined the monochloramine concentrations required to inhibit bacterial biofilms, not much is known about how bacterial biofilms develop resistance towards monochloramine. This study covers the development of resistance to monochloramine in both single species and mixed bacterial biofilms. Read More

View Article and Full-Text PDF

Development of Dimethylisoxazole-Attached Imidazo[1,2-]pyridines as Potent and Selective CBP/P300 Inhibitors.

J Med Chem 2021 May 19;64(9):5787-5801. Epub 2021 Apr 19.

Center for Green Chemistry and Department of Chemistry, University of Massachusetts Boston, 100 Morrissey Boulevard, Boston, Massachusetts 02125, United States.

The use of epigenetic bromodomain inhibitors as anticancer therapeutics has transitioned from targeting bromodomain extraterminal domain (BET) proteins into targeting non-BET bromodomains. The two most relevant non-BET bromodomain oncology targets are cyclic AMP response element-binding protein (CBP) and E1A binding protein P300 (EP300). To explore the growing CBP/EP300 interest, we developed a highly efficient two-step synthetic route for dimethylisoxazole-attached imidazo[1,2-]pyridine scaffold-containing inhibitors. Read More

View Article and Full-Text PDF

Carbon loss from boreal forest wildfires offset by increased dominance of deciduous trees.

Science 2021 04;372(6539):280-283

Center for Ecosystem Science and Society, Northern Arizona University, Flagstaff, AZ 86001, USA.

In boreal forests, climate warming is shifting the wildfire disturbance regime to more frequent fires that burn more deeply into organic soils, releasing sequestered carbon to the atmosphere. To understand the destabilization of carbon storage, it is necessary to consider these effects in the context of long-term ecological change. In Alaskan boreal forests, we found that shifts in dominant plant species catalyzed by severe fire compensated for greater combustion of soil carbon over decadal time scales. Read More

View Article and Full-Text PDF

CREB stimulates GPX4 transcription to inhibit ferroptosis in lung adenocarcinoma.

Oncol Rep 2021 Jun 13;45(6). Epub 2021 Apr 13.

Shanghai Institute of Thoracic Oncology, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, P.R. China.

Ferroptosis is a new form of regulated cell death and closely related to cancer. However, the mechanism underlying the regulation of ferroptosis in lung adenocarcinoma (LUAD) remains unclear. IB, IHC and ELISA were performed to analyze protein expression. Read More

View Article and Full-Text PDF

CREG ameliorates the phenotypic switching of cardiac fibroblasts after myocardial infarction via modulation of CDC42.

Cell Death Dis 2021 Apr 6;12(4):355. Epub 2021 Apr 6.

Cardiovascular Research Institute and Department of Cardiology, General Hospital of Northern Theater Command, Shenyang, China.

Phenotype switching of cardiac fibroblasts into myofibroblasts plays important role in cardiac fibrosis following myocardial infarction (MI). Cellular repressor of E1A-stimulated genes (CREG) protects against vascular and cardiac remodeling induced by angiotensin-II. However, the effects and mechanisms of CREG on phenotype switching of cardiac fibroblasts after MI are unknown. Read More

View Article and Full-Text PDF

The Pitfalls in the Path of Probabilistic Inference in Forensic Entomology: A Review.

Gaétan Moreau

Insects 2021 Mar 12;12(3). Epub 2021 Mar 12.

Département de biologie, Université de Moncton, Moncton, NB E1A 3E9, Canada.

To bridge the gap between experimentation and the court of law, studies in forensic entomology and other forensic sciences have to comply with a set of experimental rules to generate probabilistic inference of quality. These rules are illustrated with successional studies of insects on a decomposing substrate as the main example. The approaches that have been used in the scientific literature to solve the issues associated with successional data are then reviewed. Read More

View Article and Full-Text PDF

Insights into the Role of the Microbiota and of Short-Chain Fatty Acids in Rubinstein-Taybi Syndrome.

Int J Mol Sci 2021 Mar 31;22(7). Epub 2021 Mar 31.

Department of Health Sciences, Università degli Studi di Milano, 20142 Milan, Italy.

The short-chain fatty acid butyrate, produced by the gut microbiota, acts as a potent histone deacetylase (HDAC) inhibitor. We assessed possible ameliorative effects of butyrate, relative to other HDAC inhibitors, in in vitro and in vivo models of Rubinstein-Taybi syndrome (RSTS), a severe neurodevelopmental disorder caused by variants in the genes encoding the histone acetyltransferases CBP and p300. In RSTS cell lines, butyrate led to the patient-specific rescue of acetylation defects at subtoxic concentrations. Read More

View Article and Full-Text PDF

Small Bait Traps May Not Accurately Reflect the Composition of Necrophagous Diptera Associated to Remains.

Insects 2021 Mar 20;12(3). Epub 2021 Mar 20.

Département de Biologie, Université de Moncton, Moncton, NB E1A 3E9, Canada.

Small bait traps are beginning to emerge in forensic entomology as a new approach to sample early-colonizing necrophagous Diptera species while reducing the investment in time and energy in obtaining information. To test the hypothesis conveyed by the literature that these traps can be a substitute for whole carcasses, we simultaneously documented the Diptera assemblages visiting and colonizing domestic pig carcasses and small traps baited with pork liver. Results indicated that Diptera species occurrence and assemblage composition in the small bait traps and on the carcasses differed, while they were similar when comparing only the pig carcasses. Read More

View Article and Full-Text PDF

Are Countries of the Eastern Mediterranean Region on Track towards Meeting the World Health Assembly Target for Anemia? A Review of Evidence.

Int J Environ Res Public Health 2021 03 2;18(5). Epub 2021 Mar 2.

Nutrition and Food Sciences Department, Faculty of Agriculture and Food Sciences, American University of Beirut, Beirut 11-0236, Lebanon.

Anemia is a multifactorial condition, with a complex etiology that involves nutritional and non-nutritional factors. The misconception that iron deficiency is equivalent to anemia may mask the need to address other potential causative factors. This review paper aims to (1) assess the burden of anemia vs. Read More

View Article and Full-Text PDF

Synergistic efficacy of curcumin and anti-programmed cell death-1 in hepatocellular carcinoma.

Life Sci 2021 Mar 19:119359. Epub 2021 Mar 19.

Department of Hepatology, Qingdao No. 6 People's Hospital, Qingdao 266033, PR China. Electronic address:

Hepatocellular carcinoma (HCC) ranks near the top in the global list of malignancies causing cancer-related death. Recently, combination therapy has gained popularity in treating this cancer. We tried to investigate the efficacy of combined treatment with curcumin and anti-programmed cell death-1 (anti-PD-1) in HCC. Read More

View Article and Full-Text PDF

AXL is crucial for E1A-enhanced therapeutic efficiency of EGFR tyrosine kinase inhibitors through NFI in breast cancer.

Environ Toxicol 2021 Mar 18. Epub 2021 Mar 18.

Division of General Surgery, Department of Surgery, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan.

AXL which is a chemosensitizer protein for breast cancer cells in response to epidermal growth factor receptor-tyrosine kinase inhibitor and suppresses tumor growth. The clinical information show nuclear factor I (NFI)-C and NFI-X expression correlate with AXL expression in breast cancer patients. Following, we establish serial deletions of AXL promoter to identify regions required for Adenovirus-5 early region 1A (E1A)-mediated AXL suppression. Read More

View Article and Full-Text PDF

CREG1 improves the capacity of the skeletal muscle response to exercise endurance via modulation of mitophagy.

Autophagy 2021 Apr 18:1-17. Epub 2021 Apr 18.

Department of Cardiology and Cardiovascular Research Institute, General Hospital of Northern Theater Command, Shenyang, China.

CREG1 (cellular repressor of E1A-stimulated genes 1) is involved in tissue homeostasis and influences macroautophagy/autophagy to protect cardiovascular function. However, the physiological and pathological role of CREG1 in the skeletal muscle is not clear. Here, we established a skeletal muscle-specific knockout mouse model () by crossing the -floxed mice () with a transgenic line expressing recombinase under the muscle-specific (creatine kinase, muscle) promoter. Read More

View Article and Full-Text PDF

Chronic social stress alters protein metabolism in juvenile rainbow trout, Oncorhynchus mykiss.

J Comp Physiol B 2021 May 12;191(3):517-530. Epub 2021 Mar 12.

Département de Biologie, Université de Moncton, Moncton, NB, E1A 3E9, Canada.

When confined in pairs, juvenile rainbow trout (Oncorhynchus mykiss) form dominance hierarchies in which subordinate fish exhibit characteristic physiological changes including reduced growth rates and chronically elevated plasma cortisol concentrations. We hypothesized that alterations in protein metabolism contribute to the reduced growth rate of socially stressed trout, and predicted that subordinate trout would exhibit reduced rates of protein synthesis coupled with increases in protein degradation. Protein metabolism was assessed in dominant and subordinate fish after 4 days of social interaction, and in fish that were separated after 4 days of interaction for a 4 days recovery period, to determine whether effects on protein metabolism recovered when social stress was alleviated. Read More

View Article and Full-Text PDF

Circulating cell-free DNA as potential diagnostic tools for amyotrophic lateral sclerosis.

Neurosci Lett 2021 04 8;750:135813. Epub 2021 Mar 8.

Department of Chemistry and Biochemistry, Université de Moncton, 18 Antonine-Maillet Avenue, Moncton, New Brunswick, E1A 3E9, Canada. Electronic address:

DNA methylation has garnered much attention in recent years for its diagnostic potential in multiple conditions including cancer and neurodegenerative diseases. Conversely, advances regarding the potential diagnostic relevance of DNA methylation status have been sparse in the field of amyotrophic lateral sclerosis (ALS) even though patients diagnosed with this condition would significantly benefit from improved molecular assays aimed at furthering the current diagnostic and therapeutic options available. This review will provide an overview of the current diagnostic approaches available for ALS diagnosis and discuss the potential clinical usefulness of DNA methylation. Read More

View Article and Full-Text PDF

Characterization of Gene Isoforms and Targets Across Cell Types Reveals Highly Conserved Networks.

Front Genet 2021 22;12:613808. Epub 2021 Feb 22.

Laboratorio de Transducción de Señales y Cáncer, Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, Chile.

The SALL2 transcription factor, an evolutionarily conserved gene through vertebrates, is involved in normal development and neuronal differentiation. In disease, SALL2 is associated with eye, kidney, and brain disorders, but mainly is related to cancer. Some studies support a tumor suppressor role and others an oncogenic role for SALL2, which seems to depend on the cancer type. Read More

View Article and Full-Text PDF
February 2021

Mitochondrial physiology and responses to elevated hydrogen sulphide in two isogenic lineages of an amphibious mangrove fish.

J Exp Biol 2021 Apr 16;224(8). Epub 2021 Apr 16.

Department of Chemistry and Biochemistry, University of Moncton, Moncton, NB, Canada, E1A 3E9.

Hydrogen sulphide (H2S) is toxic and can act as a selective pressure on aquatic organisms, facilitating a wide range of adaptations for life in sulphidic environments. Mangrove rivulus (Kryptolebias marmoratus) inhabit mangrove swamps and have developed high tolerance to environmental H2S. They are hermaphroditic and can self-fertilize, producing distinct isogenic lineages with different sensitivity to H2S. Read More

View Article and Full-Text PDF

Heat Shock Protein 90 Chaperones E1A Early Protein of Adenovirus 5 and Is Essential for Replication of the Virus.

Int J Mol Sci 2021 Feb 18;22(4). Epub 2021 Feb 18.

Department of Experimental Pharmacology, Mossakowski Medical Research Institute, Polish Academy of Sciences, Pawinskiego 5, 02-106 Warsaw, Poland.

Adenovirus infections tend to be mild, but they may pose a serious threat for young and immunocompromised individuals. The treatment is complicated because there are no approved safe and specific drugs for adenovirus infections. Here, we present evidence that 17-(Allylamino)-17-demethoxygeldanamycin (17-AAG), an inhibitor of Hsp90 chaperone, decreases the rate of human adenovirus 5 (HAdV-5) replication in cell cultures by 95%. Read More

View Article and Full-Text PDF
February 2021

Insights into interaction mechanism of inhibitors E3T, E3H and E3B with CREB binding protein by using molecular dynamics simulations and MM-GBSA calculations.

SAR QSAR Environ Res 2021 Mar;32(3):221-246

School of Science, Shandong Jiaotong University, Jinan, China.

CREB binding protein (CBP) and its paralog E1A binding protein (p300) are related to the development of inflammatory diseases, cancers and other diseases, and have been potential targets for the treatment of human diseases. In this work, interaction mechanism of three small molecules E3T, E3H, and E3B with CBP was investigated by employing molecular dynamics (MD) simulations, principal component analysis (PCA), and molecular mechanics/generalized born surface area (MM-GBSA) method. The results indicate that inhibitor bindings cause the changes of movement modes and structural flexibility of CBP, and van der Waals interactions mostly drive associations of inhibitors with CBP. Read More

View Article and Full-Text PDF

Co-condensation between transcription factor and coactivator p300 modulates transcriptional bursting kinetics.

Mol Cell 2021 04 1;81(8):1682-1697.e7. Epub 2021 Mar 1.

Center for Quantitative Biology and Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; The MOE Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences, Peking University, Beijing 100871, China. Electronic address:

The coactivator p300/CREB-binding protein (CBP) regulates genes by facilitating the assembly of transcriptional machinery and by acetylating histones and other factors. However, it remains mostly unclear how both functions of p300 are dynamically coordinated during gene control. Here, we showed that p300 can orchestrate two functions through the formation of dynamic clusters with certain transcription factors (TFs), which is mediated by the interactions between a TF's transactivation domain (TAD) and the intrinsically disordered regions of p300. Read More

View Article and Full-Text PDF

Study of inhibition of germination of potato by ethylene.

Heliyon 2021 Feb 6;7(2):e06175. Epub 2021 Feb 6.

CEN, Centre d'études Nucléaires de Saclay, DEN, LRMO, bat 391, BP 91191 Gif-sur Yvette, France.

In Canada, the potato () is by far the most cultivated vegetable and plays a major nutritional role. However, during storage, the potato can easily undergo germination. In this study we have shown the inhibition potential of ethylene as an anti-germinative agent acting especially on phenols. Read More

View Article and Full-Text PDF
February 2021

Analysis of E1A domains involved in the enhancement of CDK2 activity.

Biochem Biophys Res Commun 2021 Apr 25;548:98-103. Epub 2021 Feb 25.

Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan. Electronic address:

E1A is an adenoviral protein which is expressed at the early phase after viral infection and contains four conserved regions (CR1, CR2, CR3 and CR4). Our previous work suggests that E1A facilitates the formation of cyclin A-CDK2 complex and thereby enhances CDK2 activity. However, the molecular function of E1A in CDK2 activation has been unclear. Read More

View Article and Full-Text PDF

Association between chronic psychoactive substances use and systemic inflammation: A systematic review and meta-analysis.

Neurosci Biobehav Rev 2021 Jun 24;125:208-220. Epub 2021 Feb 24.

University of St Andrews School of Medicine, Division of Population and Behavioral Science, North Haugh, St Andrews, Fife, KY16 9TF, Scotland, United Kingdom. Electronic address:

This systematic review and meta-analysis assess the change in inflammation biomarkers level among chronic psychoactive substance users. To meet the required inclusion criteria, all studies had to describe human participants with an age ≥18y., experiencing chronic psychostimulant (nicotine, amphetamine, cocaine), sedative (benzodiazepine, opioids) and/or cannabinoid use. Read More

View Article and Full-Text PDF