13 results match your criteria e-gst activity

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Effect of Air Pollution on Glutathione S-Transferase Activity and Total Antioxidant Capacity: Cross Sectional Study in Kuwait.

J Health Pollut 2020 Sep 25;10(27):200906. Epub 2020 Aug 25.

Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Yarmouk University, Irbid, Jordan.

Background: Air pollution poses a significant threat to human health worldwide. Investigating potential health impacts is essential to the development of regulations and legislation to minimize health risks.

Objectives: The aim of the present study was to investigate the potentially hazardous effect of air pollution on the Ali Sabah Al Salem residential area in Kuwait by comparing the pollution level to a control area (Al-Qirawan) by assessing two biomarkers: erythrocyte glutathione S-transferases (e-GST) and total blood antioxidant, and then correlating the activity to pollution-related oxidative stress. Read More

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September 2020

A Pilot Study of a Natural Food Supplement as New Possible Therapeutic Approach in Chronic Kidney Disease Patients.

Pharmaceuticals (Basel) 2020 Jul 10;13(7). Epub 2020 Jul 10.

PHYTOLAB (Pharmaceutical, Cosmetic, Food Supplement, Technology and Analysis)-DiSIA, University of Florence, Via U. Schiff, 6, 50019 Sesto Fiorentino, Italy.

The identification of natural bioactive compounds, able to counteract the abnormal increase of oxidative stress and inflammatory status in chronic degenerative non-communicable diseases is useful for the clinical management of these conditions. We tested an oral food supplement (OFS), chemically characterized and evaluated for in vitro and in vivo activity. Vitamin C, analyzed by High Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD), was 0. Read More

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Correlation of Blood Oxidative Stress Parameters to Indoor Radiofrequency Radiation: A Cross Sectional Study in Jordan.

Int J Environ Res Public Health 2020 06 29;17(13). Epub 2020 Jun 29.

Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Yarmouk University, Shafiq Irshidat st Irbid 21163, Jordan.

: Electromagnetic pollution is a general health concern worldwide, as cell phone towers are ubiquitous and are located adjacent to or on the roof of schools, and hospitals. However, the health risks are still inconclusive. This cross-sectional study evaluated the potential effect of electromagnetic radiation generated from various resources including cell phone towers on blood glutathione S transferase activity (e-GST) and total antioxidant activity of the Jordanian population. Read More

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Synthesis of biogenic silver nanoparticles using Althaea officinalis as reducing agent: evaluation of toxicity and ecotoxicity.

Sci Rep 2018 08 17;8(1):12397. Epub 2018 Aug 17.

Labiton - Laboratory of Bioactivity Assessment and Toxicology of Nanomaterials, University of Sorocaba, Rodovia Raposo Tavares, km 92, 18023-000, Sorocaba, São Paulo, Brazil.

Silver nanoparticles (AgNPs) are known mainly because of their bactericidal properties. Among the different types of synthesis, there is the biogenic synthesis, which allows the synergy between the nanocomposites and substances from the organism employed for the synthesis. This study describes the synthesis of AgNPs using infusion of roots (AgNpR) and extract (AgNpE) of the plant Althaea officinalis. Read More

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Erythrocyte glutathione transferase in kidney transplantation: a probe for kidney detoxification efficiency.

Cell Death Dis 2018 02 19;9(3):288. Epub 2018 Feb 19.

Department of Chemical Sciences and Technologies, University of Rome, Tor Vergata, Via della Ricerca Scientifica, 00133, Rome, Italy.

Erythrocyte glutathione transferase (e-GST) is overexpressed in case of increased blood toxicity and its level correlates with the kidney disease progression. Thus, it represents a probe of kidney efficiency against circulating toxins. We measured the activity of e-GST in patients with transplant kidney from living and cadaver donors, correlated its level to biochemical parameters of kidney function, and measured the level of oxidized albumin as a probe of oxidative stress using a new simple procedure. Read More

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February 2018

Erythrocyte glutathione transferase: a general probe for chemical contaminations in mammals.

Cell Death Discov 2016 23;2:16029. Epub 2016 May 23.

Dipartimento di Scienze e Tecnologie Chimiche, Università degli Studi di Roma 'Tor Vergata' , Rome, Italy.

Glutathione transferases (GSTs) are enzymes devoted to the protection of cells against many different toxins. In erythrocytes, the isoenzyme (e-GST) mainly present is GSTP1-1, which is overexpressed in humans in case of increased blood toxicity, as it occurs in nephrophatic patients or in healthy subjects living in polluted areas. The present study explores the possibility that e-GST may be used as an innovative and highly sensitive biomarker of blood toxicity also for other mammals. Read More

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Three week dietary intervention using apricots, pomegranate juice or/and fermented sour sobya and impact on biomarkers of antioxidative activity, oxidative stress and erythrocytic glutathione transferase activity among adults.

Nutr J 2016 05 12;15(1):52. Epub 2016 May 12.

Department of Agronomy, Faculty of Agric, Cairo University, Giza, Egypt.

Background: The beneficial effects of the polyphenol (PP) rich fruits and Lactic acid bacteria fermented foods had been reported as cost-effective strategies for health promotion. Randomized controlled trial was designed to test the hypothesis that daily intake of polyphenol rich pomegranate juice (PGJ) or/ and lactic acid bacteria fermented sobya (FS) improved selected biomarkers of relevance to heath status.

Methods: The design of the human trial consisted of 35 healthy adults, who were distributed to 5 equal groups; The first group served as control and received no supplements; the second group received fresh apricot fruits (200 g); the third (PGJ) (250 g), the fourth a mixture of PGJ (150 g) and FS (140 g) and the fifth group received (FS) (170 g). Read More

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Erythrocyte glutathione transferase: a non-antibody biomarker for systemic sclerosis, which correlates with severity and activity of the disease.

Cell Death Dis 2013 Jul 25;4:e736. Epub 2013 Jul 25.

Department of Chemical Sciences and Technologies, University of Rome 'Tor Vergata', Via della Ricerca Scientifica, 00133 Rome, Italy.

Erythrocyte glutathione transferase (e-GST) is a detoxifying enzyme hyper-expressed in nephropathic patients and used recently as a biomarker for blood toxicity. Systemic sclerosis (SSc) is characterized by endothelial dysfunction and fibrosis of the skin and internal organs. Renal involvement is frequent in SSc patients. Read More

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Erythrocyte glutathione transferase activity: a possible early biomarker for blood toxicity in uremic diabetic patients.

Acta Diabetol 2014 Apr 2;51(2):219-24. Epub 2013 Jul 2.

Nephrology and Hypertension Unit, Department of System Medicine, University of Rome "Tor Vergata", Viale Oxford 81, 00133, Rome, Italy.

Erythrocyte glutathione transferase (e-GST) displays increased activity in patients with renal damage and positive correlation with homocysteine (Hcy) in patients under maintenance hemodialysis. Here, we determined e-GST, Hcy, and erythrocyte catalase (e-CAT) in 328 patients affected by type 2 diabetes mellitus (T2DM), 61 diabetic non-nephropathic patients and 267 affected by diabetes and by chronic kidney disease (CKD) under conservative therapy subdivided into four stages according to K-DOQI lines. e-GST activity was significantly higher in all T2DM patients compared to the control group (7. Read More

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Erythrocyte glutathione transferase: a new biomarker for hemodialysis adequacy, overcoming the Kt/V(urea) dogma?

Cell Death Dis 2012 Aug 23;3:e377. Epub 2012 Aug 23.

Department of Internal Medicine, Nephrology and Hypertension Unit, 'Tor Vergata' University, Viale Oxford, Rome, Italy.

Kt/V(urea) ratio is commonly used to assess the delivered dose of dialysis in maintenance hemodialysis (MHD) patients. This parameter only reflects the efficacy of dialytic treatments in removing small toxins, but not middle and protein-bound toxins. Erythrocyte glutathione transferase (e-GST), an enzyme devoted to cell depuration against a lot of large and small toxins, is overexpressed in uremic patients. Read More

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Erythrocyte glutathione transferase: a novel biomarker to check environmental pollution hazardous for humans.

Biochem Biophys Res Commun 2012 Sep 14;426(1):71-5. Epub 2012 Aug 14.

Department of Chemical Sciences and Technologies, University of Rome Tor Vergata, 00133 Rome, Italy.

Glutathione transferase (GST) is an enzyme capable of protecting the body from a lot of toxic compounds. Previous studies demonstrated that the erythrocyte GST (e-GST) expression increases as the level of circulating toxins increases. Aim of the present study is to verify if e-GST may represent a biomarker able to signalize an environmental pollution hazardous for humans. Read More

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September 2012

Erythrocyte glutathione transferase: a potential new biomarker in chronic kidney diseases which correlates with plasma homocysteine.

Amino Acids 2012 Jul 8;43(1):347-54. Epub 2011 Oct 8.

Department of Laboratory Medicine, University of Rome Tor Vergata, Rome, Italy.

The erythrocyte glutathione S-transferase (e-GST) is a member of a superfamily of inducible enzymes involved in cell detoxification that shows an increased expression in chronic kidney disease (CKD) patients. We propose a new automated analysis procedure for e-GST activity that has been validated in 72 CKD patients and 62 maintenance hemodialysis patients (MHD). Regression analysis was carried out to assess association between e-GST activity data, main clinical variables, and plasma homocysteine (Hcy), a modified sulfur amino acid known as potential risk factor for cardiovascular disease that is increased above normal levels in more than 90% of the uremic patients. Read More

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A high pressure liquid chromatography-based assay for glutathione-S-transferase class distinction assay.

J Biochem Biophys Methods 2007 Aug 4;70(5):761-5. Epub 2007 May 4.

Department of Chemistry and Biochemistry, School of Marine Science and Technology, University of Massachusetts Dartmouth, 285 Old Westport Road, Dartmouth, MA 02747, United States.

In order to expedite the process of classification of the members of the family of glutathione-S-transferases (GSTs) high performance liquid chromatography with photodiode array detection (HPLC-PDA) was used as a means for measuring enzymatic activity. The GST chosen for the development of the HPLC-PDA technique was from equine liver (E-GST). The characterizing substrates, ethacrynic acid (EA) and bromosulfophthalein (BSP), along with previously gathered characterization data allowed for the distinction of alpha, mu or pi-class enzymes. Read More

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