661,644 results match your criteria drug resistant

Development of an in vitro test method to simulate intra-operative impaction loading on lumbar intervertebral body fusion devices.

J Biomech 2021 Apr 5;121:110412. Epub 2021 Apr 5.

U.S. Food and Drug Administration, Center for Devices and Radiological Health, Office of Science and Engineering Laboratories, Division of Applied Mechanics, Silver Spring, MD 20993, USA.

Intervertebral body fusion devices (IBFDs) are commonly used in the treatment of various spinal pathologies. Intra-operative fractures of polyether-ether-ketone (PEEK) implants have been reported in the literature and to the FDA as device-related adverse events. The device and/or implant inserter failures typically occur during device impaction into the disc space and require implant removal and replacement. Read More

View Article and Full-Text PDF

Extracellular vesicles and leishmaniasis: Current knowledge and promising avenues for future development.

Mol Immunol 2021 Apr 16;135:73-83. Epub 2021 Apr 16.

Infectious Diseases and Immunology in Global Health Program (IDIGH), The Research Institute of the McGill University Health Centre, Montréal, QC, Canada; Departments of Medicine, Microbiology and Immunology, Faculty of Medicine, McGill University, Montréal, QC, Canada. Electronic address:

Extracellular vesicles (EVs) are small, membrane-bound "delivery trucks" that are present in the extracellular environment, including biological fluids. EVs are capable of inducing changes in the physiological status of neighboring cells through the transfer of key macromolecules, and are thought to play a role in a number of pathological processes. Leishmaniasis, caused by the protozoan parasite Leishmania, is an important example. Read More

View Article and Full-Text PDF

TMPyP-bound guanosine-borate supramolecular hydrogel as smart hemoperfusion device with real-time visualized/electrochemical bi-modal monitoring for selective blood lead elimination.

Biosens Bioelectron 2021 Apr 7;184:113230. Epub 2021 Apr 7.

Guangdong Provincial Engineering and Technological Research Center for Drug Carrier Development, Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China. Electronic address:

Blood lead poisoning is a universal and severe health problem that greatly threatens human health in various industries. Elimination of blood lead relying on chelating agents and combination with hemoperfusion adsorbents has achieved considerable progress, but it is still suffering from the compromised selectivity of adsorbents as well as in absence of real time monitoring during treatment. Herein, we proposed a selective blood lead adsorbent integrated with real-time visualized/electrochemical bi-modal monitoring based on TMPyP-bound guanosine-borate (GB) supramolecular hydrogel as potential smart hemoperfusion device. Read More

View Article and Full-Text PDF

Stereoelectroencephalography-guided radiofrequency thermocoagulation for hypothalamic hamartoma: Electroclinical patterns and the relationship with surgical prognosis.

Epilepsy Behav 2021 Apr 16;118:107957. Epub 2021 Apr 16.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China; Department of Stereotactic and Functional Neurosurgery, Beijing Neurosurgical Institute, Beijing, China. Electronic address:

Background: Radiofrequency thermocoagulation (RFTC) guided by stereo-electroencephalography (SEEG) has been proved to be a safe method to reduce seizure frequency in patients with drug-resistant epilepsy. However, there are few reports addressing the value and safety of this procedure in hypothalamic hamartoma (HH).

Objective: To present the results of our experience using SEEG-guided RFTC in HH patients with drug-resistant epilepsy, and identify outcome predictors. Read More

View Article and Full-Text PDF

Awake epilepsy surgery in patients with focal cortical dysplasia.

World Neurosurg 2021 Apr 16. Epub 2021 Apr 16.

Department of Neurosurgery, University Hospital "St. Ivan Rilski", Sofia, Bulgaria.

Background: Awake craniotomy (AC) and direct electrical stimulation (DES) emerged together with epilepsy surgery more than 80 years ago. The goal of our study was to evaluate the benefits of awake surgery in patients with drug-resistant epilepsy due to focal cortical dysplasia (FCD) affecting eloquent areas.

Methods: Our material included 95 patients with drug-resistant epilepsy and FCD, who were operated on between January 2009 and December 2018. Read More

View Article and Full-Text PDF

Genome wide CRISPR screening reveals a role for sialylation in the tumorigenesis and chemoresistance of acute myeloid leukemia cells.

Cancer Lett 2021 Apr 16. Epub 2021 Apr 16.

Department of Convergence Medicine, Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea. Electronic address:

Aberrant activation of cytokine and growth factor signal transduction pathways confers enhanced survival and proliferation properties to acute myeloid leukemia (AML) cells. However, the mechanisms underlying the deregulation of signaling pathways in leukemia cells are unclear. To identify genes capable of independently supporting cytokine-independent growth, we employed a genome-wide CRISPR/Cas9-mediated loss-of-function screen in GM-CSF-dependent human AML TF-1 cells. Read More

View Article and Full-Text PDF

Sulforaphane inhibits PRMT5 and MEP50 function to suppress the mesothelioma cancer cell phenotype.

Mol Carcinog 2021 Apr 19. Epub 2021 Apr 19.

Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland, USA.

Mesothelioma is a highly aggressive cancer of the mesothelial lining that is caused by exposure to asbestos. Surgical resection followed by chemotherapy is the current treatment strategy, but this is marginally successful and leads to drug-resistant disease. We are interested in factors that maintain the aggressive mesothelioma cancer phenotype as therapy targets. Read More

View Article and Full-Text PDF

The many facets of Notch signaling in breast cancer: toward overcoming therapeutic resistance.

Genes Dev 2020 Nov;34(21-22):1422-1438

Department of Biomedical Sciences, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.

Breast cancer is the second leading cause of cancer-related death in women and is a complex disease with high intratumoral and intertumoral heterogeneity. Such heterogeneity is a major driving force behind failure of current therapies and development of resistance. Due to the limitations of conventional therapies and inevitable emergence of acquired drug resistance (chemo and endocrine) as well as radio resistance, it is essential to design novel therapeutic strategies to improve the prognosis for breast cancer patients. Read More

View Article and Full-Text PDF
November 2020

Ciprofloxacin-Resistant Displays Enhanced Resistance and Virulence in Iron-Restricted Conditions.

J Proteome Res 2021 Apr 19. Epub 2021 Apr 19.

MOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, Jinan University, Guangzhou 510632, China.

The unreasonable misuse of antibiotics has led to the emergence of large-scale drug-resistant bacteria, seriously threatening human health. Compared with drug-sensitive bacteria, resistant bacteria are difficult to clear by host immunity. To fully explore the adaptive mechanism of resistant bacteria to the iron-restricted environment, we performed data-independent acquisition-based quantitative proteomics on ciprofloxacin (CIP)-resistant (CIP-R) in the presence or absence of iron. Read More

View Article and Full-Text PDF

Mechano-Induced Assembly of a Nanocomposite for "Press-N-Go" Coatings with Highly Efficient Surface Disinfection.

ACS Appl Mater Interfaces 2021 Apr 19. Epub 2021 Apr 19.

Department of Biomedical Sciences, City University of Hong Kong, Kowloon 999077, P. R. China.

Using antimicrobial coatings to control the spread of pathogenic microbes is appreciated in public and healthcare settings, but the performance of most antimicrobial coatings could not fulfill the increasing requirements, particularly the ease of preparation, high durability, rapid response, and high killing efficiency. Herein, we develop a new type of mechano-induced assembly of nanocomposite coating by simple "Press-N-Go" procedures on various substrates such as glassware, gloves, and fabrics, in which the coating shows strong adhesion, high shear stability, and high stiffness, making it durable in daily use to withstand common mechanical deformation and scratches. The coating also shows remarkable disinfection effectiveness over 99. Read More

View Article and Full-Text PDF

In vitro evaluation of loaded chitosan membranes for pain relief and infection prevention.

J Biomed Mater Res B Appl Biomater 2021 Apr 19. Epub 2021 Apr 19.

Department of Biomedical Engineering, University of Memphis, Memphis, Tennessee, USA.

Wounds resulting from surgeries, implantation of medical devices, and musculoskeletal trauma result in pain and can also result in infection of damaged tissue. Up to 80% of these infections are due to biofilm formation either on the surface of implanted devices or on surrounding wounded tissue. Bacteria within a biofilm have intrinsic growth and development characteristics that allow them to withstand up to 1,000 times the minimum inhibitory concentration of antibiotics, demonstrating the need for new therapeutics to prevent and treat these infections. Read More

View Article and Full-Text PDF

Antibacterial activities of hexadecanoic acid methyl ester and green-synthesized silver nanoparticles against multidrug-resistant bacteria.

J Basic Microbiol 2021 Apr 19. Epub 2021 Apr 19.

Department of Botany and Microbiology, Faculty of Science, Menoufia University, Menoufia, Egypt.

Antibacterial drug resistance is considered one of the biggest threats to human health worldwide, and the overuse of antibiotics accelerates this problem. Multidrug-resistant (MDR) bacteria are becoming harder to treat as the antibiotics used to treat them become less effective. Therefore, it is necessary to evaluate novel methods to control MDR bacteria. Read More

View Article and Full-Text PDF

Low-dimensional nanomaterials for antibacterial applications.

J Mater Chem B 2021 Apr 19. Epub 2021 Apr 19.

School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan RD, Shanghai 200240, China.

The excessive use of antibiotics has led to a rise in drug-resistant bacteria. These "superbugs" are continuously emerging and becoming increasingly harder to treat. As a result, new and effective treatment protocols that have minimal risks of generating drug-resistant bacteria are urgently required. Read More

View Article and Full-Text PDF

The role of the human gut microbiota in colonization and infection with multidrug-resistant bacteria.

Gut Microbes 2021 Jan-Dec;13(1):1-13

Department of Internal Medicine, Infectious Diseases, University Hospital Frankfurt, Goethe University Frankfurt, Frankfurt Am Main, Germany.

About 100 years ago, the first antibiotic drug was introduced into health care. Since then, antibiotics have made an outstanding impact on human medicine. However, our society increasingly suffers from collateral damage exerted by these highly effective drugs. Read More

View Article and Full-Text PDF

Synergistic activity of diclofenac sodium with oxacillin against planktonic cells and biofilm of methicillin-resistant strains.

Future Microbiol 2021 Apr 19. Epub 2021 Apr 19.

Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.

To evaluate the activity of diclofenac sodium and synergism with oxacillin against clinical strains of SARM in plactonic cells, antibiofilm and biofilm. Synergism activity was assessed using the fractional inhibitory concentration index and its possible mechanism of action by flow cytometry. The synergistic activity of diclofenac sodium with oxacillin was observed against plactonic cells, antibiofilm and in biofilm formed from clinical methicillin-resistant strains. Read More

View Article and Full-Text PDF

Effect of Increased Potassium Intake on Adrenal Cortical and Cardiovascular Responses to Angiotensin II: A Randomized Crossover Study.

J Am Heart Assoc 2021 Apr 19:e018716. Epub 2021 Apr 19.

Department of Medicine Amager Hvidovre Hospital in Glostrup University of Copenhagen Glostrup Denmark.

Background Increased potassium intake lowers blood pressure in patients with hypertension, but increased potassium intake also elevates plasma concentrations of the blood pressure-raising hormone aldosterone. Besides its well-described renal effects, aldosterone is also believed to have vascular effects, acting through mineralocorticoid receptors present in endothelial and vascular smooth muscle cells, although mineralocorticoid receptors-independent actions are also thought to be involved. Methods and Results To gain further insight into the effect of increased potassium intake and potassium-stimulated hyperaldosteronism on the human cardiovascular system, we conducted a randomized placebo-controlled double-blind crossover study in 25 healthy normotensive men, where 4 weeks treatment with a potassium supplement (90 mmol/day) was compared with 4 weeks on placebo. Read More

View Article and Full-Text PDF

H, C and N chemical shift assignments of the C-terminal domain of human UDP-Glucuronosyltransferase 2B7 (UGT2B7-C).

Biomol NMR Assign 2021 Apr 18. Epub 2021 Apr 18.

Department of Pathology and Cell Biology, Institute of Research in Immunology and Cancer (IRIC), Université de Montréal, Pavillion Marcelle-Coutu, Chemin Polytechnique, Montreal, QC, Canada.

The human UDP-glucuronosyltransferase (UGT) family of enzymes catalyze the covalent addition of glucuronic acid to a wide range of compounds, generally rendering them inactive. Although important for clearance of environmental toxins and metabolites, UGT activation can lead to inappropriate glucuronidation of therapeutics underlying drug resistance. Indeed, 50% of medications are glucuronidated. Read More

View Article and Full-Text PDF

Forcing dividing cancer cells to die; low-dose drug combinations to prevent spindle pole clustering.

Apoptosis 2021 Apr 19. Epub 2021 Apr 19.

School of Pharmaceutical Sciences, Faculty of Sciences, University of Geneva, Rue Michel-Servet 1, CMU, 1211, Geneva 4, Switzerland.

Mitosis, under the control of the microtubule-based mitotic spindle, is an attractive target for anti-cancer treatments, as cancer cells undergo frequent and uncontrolled cell divisions. Microtubule targeting agents that disrupt mitosis or single molecule inhibitors of mitotic kinases or microtubule motors kill cancer cells with a high efficacy. These treatments have, nevertheless, severe disadvantages: they also target frequently dividing healthy tissues, such as the haematopoietic system, and they often lose their efficacy due to primary or acquired resistance mechanisms. Read More

View Article and Full-Text PDF

Controllable mixed-charged co-assembly of dendritic lipopeptides into invisible capsid-like nanoparticles as potential drug carriers.

Chem Commun (Camb) 2021 Apr 19. Epub 2021 Apr 19.

College of Biology, Hunan University, Changsha, Hunan 410082, P. R. China.

The feature of invisibility is vital in drug nanocarriers for prolonging blood transportation, with this generating excellent resistance to protein adsorption and clearance from the body. In this work, we report a well-designed molecular and supramolecular strategy for precisely developing mixed-charged nanoparticles with resistance to protein adsorption. We constructed anionic dendritic lipopeptides (ADLs) and cationic dendritic lipopeptides (CDLs) with eight carboxyl or amino groups as terminal groups. Read More

View Article and Full-Text PDF

Hidden suppressive interactions are common in higher-order drug combinations.

iScience 2021 Apr 26;24(4):102355. Epub 2021 Mar 26.

Ecology and Evolutionary Biology, University of California, Los Angeles, 90095, USA.

The rapid increase of multi-drug resistant bacteria has led to a greater emphasis on multi-drug combination treatments. However, some combinations can be suppressive-that is, bacteria grow faster in some drug combinations than when treated with a single drug. Typically, when studying interactions, the overall effect of the combination is only compared with the single-drug effects. Read More

View Article and Full-Text PDF

Green Synthesis of Zinc Oxide (ZnO) Nanoparticles Using Aqueous Fruit Extracts of : Their Characterizations and Biological and Environmental Applications.

ACS Omega 2021 Apr 30;6(14):9709-9722. Epub 2021 Mar 30.

Department of Biotechnology, Bacha Khan University, Charsadda 24460,KPK, Pakistan.

In the present work, bioaugmented zinc oxide nanoparticles (ZnO-NPs) were prepared from aqueous fruit extracts of . The ZnO-NPs were characterized by different techniques such as X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, ultraviolet (UV) spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), and thermogravimetric analysis (TGA). The crystallites exhibited a mean size of 41. Read More

View Article and Full-Text PDF

Intelligent nanogels with self-adaptive responsiveness for improved tumor drug delivery and augmented chemotherapy.

Bioact Mater 2021 Oct 24;6(10):3473-3484. Epub 2021 Mar 24.

College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China.

For cancer nanomedicine, the main goal is to deliver therapeutic agents effectively to solid tumors. Here, we report the unique design of self-adaptive ultrafast charge-reversible chitosan-polypyrrole nanogels (CH-PPy NGs) for enhanced tumor delivery and augmented chemotherapy. CH was first grafted with PPy to form CH-PPy polymers that were used to form CH-PPy NGs through glutaraldehyde cross-linking a miniemulsion method. Read More

View Article and Full-Text PDF
October 2021

Tuberculosis Scientific Conferences in Peru: Sharing local evidence for local decisions.

J Clin Tuberc Other Mycobact Dis 2021 May 26;23:100232. Epub 2021 Mar 26.

Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Av. Honorio Delgado 430, San Martin de Porres, 15102 Lima, Peru.

Introduction: International conferences on tuberculosis (TB) have been held since the 19th century. In Peru, the TB Scientific Conferences have been held annually in Lima since 2013 as a means of developing a national TB research network and setting the stage for researchers and institutions to present and share new findings from studies conducted in Peru.

Methods: Systematization of information on speakers and presentations from TB Scientific Conferences in Peru. Read More

View Article and Full-Text PDF

Hepatitis B virus resistance to tenofovir: fact or fiction? A systematic literature review and structural analysis of drug resistance mechanisms.

Wellcome Open Res 2020 29;5:151. Epub 2020 Jun 29.

Nuffield Department of Medicine, University of Oxford, Medawar Building, South Parks Road, Oxford, OX1 3SY, UK.

Tenofovir (TFV) is a widely used treatment for chronic hepatitis B virus (HBV) infection. There is a high genetic barrier to the selection of TFV resistance-associated mutations (RAMs), but the distribution and clinical significance of TFV RAMs are not well understood. We here present assimilated evidence for putative TFV RAMs with the aims of cataloguing and characterising mutations that have been reported, and starting to develop insights into mechanisms of resistance. Read More

View Article and Full-Text PDF

Prolonged use of bedaquiline in two patients with pulmonary extensively drug-resistant tuberculosis: Two case reports.

World J Clin Cases 2021 Apr;9(10):2326-2333

Department of Tuberculosis, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, China.

Background: Bedaquiline is among the prioritized drugs recommended by the World Health Organization for the treatment of extensively drug-resistant tuberculosis (XDR-TB). Many patients have not achieved better clinical improvement after bedaquiline is stopped at 24 wk. However, there is no recommendation or guideline on bedaquiline administration beyond 24 wk, which is an important consideration when balancing the benefit of prognosis for XDR-TB against the uncertain safety concerning the newer antibiotics. Read More

View Article and Full-Text PDF

Distribution and drug resistance of pathogens in burn patients in China from 2006 to 2019.

World J Clin Cases 2021 Apr;9(10):2228-2237

Department of Burns and Plastic Surgery, Beijing Jishuitan Hospital, Beijing 100035, China.

Background: In this study, recent trends in the distribution and drug resistance of pathogenic bacteria isolated from patients treated at a burn ward between 2006 and 2019 were investigated.

Aim: To develop more effective clinical strategies and techniques for the prevention and treatment of bacterial infections in burn patients.

Methods: Clinical samples with positive bacteria were collected from patients at the burn ward in Beijing Jishuitan Hospital in China between January 2006 and December 2019. Read More

View Article and Full-Text PDF

The Social and Material Life of Antimicrobial Clay: Exploring Antimicrobial Resistance, Medicines' Materiality, and Medicines Optimization.

Front Sociol 2020 30;5:26. Epub 2020 Apr 30.

Department of Biosciences, Durham University, Durham, United Kingdom.

While sociologists have made significant theoretical contributions to the antimicrobial resistance (AMR) debate, little attention has been given to the antimicrobial products themselves. Here we advocate a significant new direction which centers on the social and material life of antimicrobials, specifically on what they are made from and how this affects their use. This focus is timely because, in the context of declining efficacy of biomedical antibiotics, diverse materials are increasingly taking center stage in research and drug discovery as potential agents for new antimicrobial treatments. Read More

View Article and Full-Text PDF

New Machine, Old Cough: Technology and Tuberculosis in Patna.

Vaibhav Saria

Front Sociol 2020 3;5:18. Epub 2020 Apr 3.

Department of Gender, Sexuality, and Women's Studies, Simon Fraser University, Burnaby, BC, Canada.

In 2013, a new technology, GeneXpert, was introduced in India, which, in addition to testing for TB, could also diagnose whether the detected strain was drug resistant. By detecting the bacterium more effectively than other available tests and simultaneously testing for resistance, GeneXpert promised to reduce the delay in diagnosis and hence ineffective treatments. The new test was introduced to multiple cities via a coalition that included global health funding bodies, the government of India, the World Health Organization, and non-governmental organizations. Read More

View Article and Full-Text PDF

High Levels of Antibiotic Resistance in Isolates From Diseased Livestock.

Front Vet Sci 2021 1;8:652351. Epub 2021 Apr 1.

Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Malaysia.

Overuse of antimicrobials in livestock health and production beyond therapeutic needs has been highlighted in recent years as one of the major risk factors for the acceleration of antimicrobial resistance (AMR) of bacteria in both humans and animals. While there is an abundance of reports on AMR in clinical isolates from humans, information regarding the patterns of resistance in clinical isolates from animals is scarce. Hence, a situational analysis of AMR based on clinical isolates from a veterinary diagnostic laboratory was performed to examine the extent and patterns of resistance demonstrated by isolates from diseased food animals. Read More

View Article and Full-Text PDF

Pharmacokinetic/Pharmacodynamic Dosage Individualization of Suppressive Beta-Lactam Therapy Administered by Subcutaneous Route in Patients With Prosthetic Joint Infection.

Front Med (Lausanne) 2021 31;8:583086. Epub 2021 Mar 31.

Centre interrégional de référence pour la prise en charge des infections ostéo-articulaires complexes (CRIOAc Lyon), Hospices Civils de Lyon, Lyon, France.

Suppressive parenteral antibiotic therapy with beta-lactams may be necessary in patients with Gram-negative bone and joint infection (BJI). Subcutaneous drug administration can facilitate this therapy in outpatient setting, but there is limited information about this practice. We have developed an original approach for drug dosing in this context, based on therapeutic drug monitoring (TDM) and pharmacokinetic/pharmacodynamic (PK/PD) principles. Read More

View Article and Full-Text PDF