2,151 results match your criteria downregulate transcription


miR-221-3p and miR-222-3p regulate the SOCS3/STAT3 signaling pathway to downregulate the expression of NIS and reduce radiosensitivity in thyroid cancer.

Exp Ther Med 2021 Jun 19;21(6):652. Epub 2021 Apr 19.

Department of Nuclear Medicine, Key Laboratory of Head and Neck Cancer Translational Research of Zhejiang Province, Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Hangzhou, Zhejiang 310021, P.R. China.

The expression levels of microRNA (miR)-221-3p and miR-222-3p in thyroid cancer have been found to be upregulated compared with those in normal tissues. The present study aimed to determine the effects and potential underlying mechanisms of miR-221-3p and miR-222-3p on the regulation of radioactive iodine (I) uptake and radiosensitivity of thyroid cancer cells. The potential regulatory target genes of miR-221-3p and miR-222-3p were predicted by bioinformatics analysis, and reverse transcription-quantitative polymerase chain reaction was used to verify miR-221-3p, miR-222-3p and target gene expression levels in thyroid cancer tissues and cell lines. Read More

View Article and Full-Text PDF

Gallic acid suppresses colon cancer proliferation by inhibiting SRC and EGFR phosphorylation.

Exp Ther Med 2021 Jun 16;21(6):638. Epub 2021 Apr 16.

School of Life Sciences, Yantai University, Yantai, Shandong 264005, P.R. China.

The aim of the present study was to investigate the effects of gallic acid (GA) on the proliferation and apoptosis of colon cancer cells and to further clarify the mechanism of GA function associated with SRC and EGFR phosphorylation. HCT116 and HT29 cells were treated with different concentrations of GA for 24 h. Cell proliferation and apoptosis were analyzed using plate clone formation and flow cytometry assays, respectively. Read More

View Article and Full-Text PDF

Upregulation of brain cholesterol levels inhibits mitophagy in Alzheimer disease.

Autophagy 2021 May 4:1-3. Epub 2021 May 4.

Department of Cell Death and Proliferation, Institut d'Investigacions Biomèdiques de Barcelona (IIBB-CSIC), Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.

Mitochondrial dysfunction is behind several neurodegenerative diseases, including Alzheimer disease (AD). Accumulation of damaged mitochondria is already observed at the early stages of AD and has been linked to impaired mitophagy, but the mechanisms underlying this alteration are still not fully known. In our recent study, we show that intracellular cholesterol enrichment can downregulate amyloid beta (Aβ)-induced mitophagy. Read More

View Article and Full-Text PDF

Targeting the EMT transcription factor Snail overcomes resistance to osimertinib in EGFR-mutant non-small cell lung cancer.

Thorac Cancer 2021 May 4. Epub 2021 May 4.

Department of Oncology, Tianjin Medical University General Hospital, Tianjin, China.

Background: The resistance mechanism of the third generation of epidermal growth factor (EGFR) tyrosine kinase inhibitor (TKI) osimertinib is complex. Epithelial mesenchymal transition (EMT) is a common mechanism of EGFR-TKI acquired resistance. Snail is an important transcription factor related to EMT. Read More

View Article and Full-Text PDF

miR-204-5p and miR-211 Synergistically Downregulate the α-Casein Content and Contribute to the Lower Allergy of Goat Milk.

J Agric Food Chem 2021 May 3;69(18):5353-5362. Epub 2021 May 3.

Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang, Shaanxi 712100, P. R. China.

α-Casein (encoded by the 11 gene) is associated with higher rates of allergy than other milk protein components for humans. microRNAs (miRNAs) as small noncoding RNA molecules regulate gene expression and influence diverse biological processes. However, little is known about the regulation of milk protein synthesis by miRNAs in ruminants. Read More

View Article and Full-Text PDF

The Diarylheptanoid Curcumin Induces MYC Inhibition and Cross-Links This Oncoprotein to the Coactivator TRRAP.

Front Oncol 2021 15;11:660481. Epub 2021 Apr 15.

Institute of Biochemistry and Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck, Innsbruck, Austria.

The c-Myc protein (MYC) is a transcription factor with strong oncogenic potential controlling fundamental cellular processes. In most human tumors, MYC is overexpressed by enhanced transcriptional activation, gene amplification, chromosomal rearrangements, or increased protein stabilization. To pharmacologically suppress oncogenic MYC functions, multiple approaches have been applied either to inhibit transcriptional activation of the endogenous gene, or to interfere with biochemical functions of aberrantly activated MYC. Read More

View Article and Full-Text PDF

REGγ regulates hair cycle by activating Lgr5 positive hair follicle stem cells.

J Dermatol Sci 2021 Apr 18. Epub 2021 Apr 18.

Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, School of Life Sciences, East China Normal University, Shanghai, China. Electronic address:

Background: REGγ acts as a proteasome activating factor mediating proteasome degradation of substrate proteins in an ATP and ubiquitination independent manner and also as an important regulator of cell cycle, proliferation and apoptosis. Hair cycle involves dynamic, continuous morphological changes of three stages (anagen, catagen and telogen).

Objective: The function of REGγ in hair cycling is still unclear. Read More

View Article and Full-Text PDF

Role of aryl hydrocarbon receptor in central nervous system tumors: Biological and therapeutic implications.

Oncol Lett 2021 Jun 11;21(6):460. Epub 2021 Apr 11.

Laboratorio de Investigación en Patología Experimental, Hospital Infantil de México Federico Gómez, Mexico City 06720, México.

Aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor, whose canonical pathway mainly regulates the genes involved in xenobiotic metabolism. However, it can also regulate several responses in a non-canonical manner, such as proliferation, differentiation, cell death and cell adhesion. AhR plays an important role in central nervous system tumors, as it can regulate several cellular responses via different pathways. Read More

View Article and Full-Text PDF

Electroacupuncture Alleviates Inflammation of Dry Eye Diseases by Regulating the 7nAChR/NF-B Signaling Pathway.

Oxid Med Cell Longev 2021 9;2021:6673610. Epub 2021 Apr 9.

Ophthalmology Department of Traditional Chinese Medicine, The First Clinical Medical College, Nanjing University of Traditional Chinese Medicine, Nanjing City, Jiangsu Province 210023, China.

Purpose: We tried to investigate whether electroacupuncture (EA) can reduce inflammation of dry eye disease (DED) by regulating 7nAChR and inhibiting the NF-B signaling pathway.

Methods: Healthy New Zealand white rabbits were treated with scopolamine hydrobromide (Scop) for 21 consecutive days to establish the DED animal model. After 21 days, EA, fluorometholone (Flu), and 7nAChR antagonist (-BGT) treatments were performed, and the Scop injection was continued until day 35. Read More

View Article and Full-Text PDF

Pro-angiogenic effect of exosomal microRNA-103a in mice with rheumatoid arthritis via the downregulation of hepatocyte nuclear factor 4 alpha and activation of the JAK/STAT3 signaling pathway.

J Biol Regul Homeost Agents 2021 Apr 23;35(2). Epub 2021 Apr 23.

Department of Rheumatology and Immunology, Tianjin Medical University General Hospital, Tianjin, P.R. China.

Rheumatoid arthritis (RA) is characterized by inflammation of the synovial membrane, accompanied by hyperplasia and neo-angiogenesis, which promote local inflammation. Macrophage-derived exosomes have been reported to enhance inflammation and the immune response. In the present study, we identified a novel exosomal microRNA (miR)-103a, which aids in the regulation of inflammation and angiogenesis in mice with RA, and attempted to identify the underlying mechanism. Read More

View Article and Full-Text PDF

Interferon Regulatory Factor 5 siRNA-Loaded Folate-Modified Cationic Liposomes for Acute Lung Injury Therapy.

J Biomed Nanotechnol 2021 Mar;17(3):466-476

Acute lung injury/acute respiratory distress syndrome (ALI/ARDS) is an overwhelming pulmonary inflammation with limited clinical treatment strategies. Interferon regulatory factor 5 (IRF5) is a crucial regulator of inflammation factors, which can be upregulated under an inflammatory state and related to the efferocytosis of macrophages. Herein, IRF5 was knockdown by small interfering RNA (siIRF5) to promote the anti-inflammatory effect of macrophages. Read More

View Article and Full-Text PDF

Reconditioning the Neurogenic Niche of Adult Non-human Primates by Antisense Oligonucleotide-Mediated Attenuation of TGFβ Signaling.

Neurotherapeutics 2021 Apr 15. Epub 2021 Apr 15.

Department of Neurology, University Hospital Regensburg, Regensburg, Germany.

Adult neurogenesis is a target for brain rejuvenation as well as regeneration in aging and disease. Numerous approaches showed efficacy to elevate neurogenesis in rodents, yet translation into therapies has not been achieved. Here, we introduce a novel human TGFβ-RII (Transforming Growth Factor-Receptor Type II) specific LNA-antisense oligonucleotide ("locked nucleotide acid"-"NVP-13"), which reduces TGFβ-RII expression and downstream receptor signaling in human neuronal precursor cells (ReNcell CX® cells) in vitro. Read More

View Article and Full-Text PDF

Colloidal Particles in Tuna Head Soup: Chemical Localization, Structural Change, and Antioxidant Property.

Front Nutr 2021 29;8:638390. Epub 2021 Mar 29.

College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, China.

In this work, chemical localization, structural changes, and antioxidant properties of tuna colloidal particles (TCPs) in boiling tuna head soup were examined. The results demonstrated that TCPs might be core-shell colloidal spherical structures. The hydrophobic core consisted of triglycerides and chloride ions. Read More

View Article and Full-Text PDF

Tumor suppressor role of hsa_circ_0035445 in gastric cancer.

J Clin Lab Anal 2021 Apr 8:e23727. Epub 2021 Apr 8.

The Affiliated People's Hospital, Ningbo University, Ningbo, China.

Circular RNAs (circRNAs) are closely related to the occurrence and development of cancers. However, the roles of circRNAs in gastric cancer are largely unknown. Total 104 pairs of gastric cancer tissues and non-cancer tissues, fasting plasma of 42 healthy people and 42 gastric cancer patients' one day before operation and 10 days after operation were collected. Read More

View Article and Full-Text PDF

Integrated genomic analysis reveals key features of long undecoded transcript isoform-based gene repression.

Mol Cell 2021 Mar 26. Epub 2021 Mar 26.

Department of Molecular and Cell Biology, Barker Hall, University of California, Berkeley, Berkeley, CA 94720, USA. Electronic address:

Long undecoded transcript isoforms (LUTIs) represent a class of non-canonical mRNAs that downregulate gene expression through the combined act of transcriptional and translational repression. While single gene studies revealed important aspects of LUTI-based repression, how these features affect gene regulation on a global scale is unknown. Using transcript leader and direct RNA sequencing, here, we identify 74 LUTI candidates that are specifically induced in meiotic prophase. Read More

View Article and Full-Text PDF

The functions of repressor element 1-silencing transcription factor in models of epileptogenesis and post-ischemia.

Metab Brain Dis 2021 Apr 4. Epub 2021 Apr 4.

School of Biomedical Sciences, Faculty of Biological Sciences, The University of Leeds, Leeds, LS2 9JT, UK.

Epilepsy is a debilitating neurological disorder characterised by recurrent seizures for which 30% of patients are refractory to current treatments. The genetic and molecular aetiologies behind epilepsy are under investigation with the goal of developing new epilepsy medications. The transcriptional repressor REST (Repressor Element 1-Silencing Transcription factor) is a focus of interest as it is consistently upregulated in epilepsy patients and following brain insult in animal models of epilepsy and ischemia. Read More

View Article and Full-Text PDF

Oxytocin Downregulates the Ca1.2 L-Type Ca Channel via Gi/cAMP/PKA/CREB Signaling Pathway in Cardiomyocytes.

Membranes (Basel) 2021 Mar 25;11(4). Epub 2021 Mar 25.

Department of Pathophysiology, Oita University School of Medicine, Oita 879-5593, Japan.

Oxytocin (OT) and its receptor (OTR) are expressed in the heart and are involved in the physiological cardiovascular functional system. Although it is known that OT/OTR signaling is cardioprotective by reducing the inflammatory response and improving cardiovascular function, the role of OT in the cardiac electrical excitation modulation has not been clarified. This study investigates the molecular mechanism of the action of OT on cardiomyocyte membrane excitation focusing on the L-type Ca channel. Read More

View Article and Full-Text PDF

Regulation of Osteoblast Differentiation by Cytokine Networks.

Int J Mol Sci 2021 Mar 11;22(6). Epub 2021 Mar 11.

Department of Microbiology and Molecular Biology, Chungnam National University, Daejeon 34134, Korea.

Osteoblasts, which are bone-forming cells, play pivotal roles in bone modeling and remodeling. Osteoblast differentiation, also known as osteoblastogenesis, is orchestrated by transcription factors, such as runt-related transcription factor 1/2, osterix, activating transcription factor 4, special AT-rich sequence-binding protein 2 and activator protein-1. Osteoblastogenesis is regulated by a network of cytokines under physiological and pathophysiological conditions. Read More

View Article and Full-Text PDF

Astaxanthin mediated regulation of VEGF through HIF1α and XBP1 signaling pathway: An insight from ARPE-19 cell and streptozotocin mediated diabetic rat model.

Exp Eye Res 2021 May 28;206:108555. Epub 2021 Mar 28.

Department of Biochemistry, CSIR-Central Food Technological Research Institute, Mysore, 570020, Karnataka, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India. Electronic address:

Breakdown of outer blood-retina barrier (BRB) has been associated with the pathogenesis of diabetic retinopathy (DR) and diabetic macular edema (DME). Vascular endothelial growth factor (VEGF) might play a detrimental role in the pathogenesis of DME, a major clinical manifestation of DR. In the present study, we investigated the inhibitory mechanism of astaxanthin on VEGF and its upstream signaling pathways under in vitro and in vivo conditions. Read More

View Article and Full-Text PDF

A Bifan Motif Shaped by ArsR1, ArsR2, and Their Cognate Promoters Frames Arsenic Tolerance of .

Front Microbiol 2021 12;12:641440. Epub 2021 Mar 12.

Systems Biology Department, Centro Nacional de Biotecnología-CSIC, Madrid, Spain.

Prokaryotic tolerance to inorganic arsenic is a widespread trait habitually determined by operons encoding an As (III)-responsive repressor (ArsR), an As (V)-reductase (ArsC), and an As (III)-export pump (ArsB), often accompanied by other complementary genes. Enigmatically, the genomes of many environmental bacteria typically contain two or more copies of this basic genetic device . To shed some light on the logic of such apparently unnecessary duplication(s) we have inspected the regulation-together and by separate-of the two clusters borne by the soil bacterium strain KT2440, in particular the cross talk between the two repressors ArsR1/ArsR2 and the respective promoters. Read More

View Article and Full-Text PDF

Dynamic regulation of anterior-posterior patterning genes in living Drosophila embryos.

Authors:
Takashi Fukaya

Curr Biol 2021 Mar 23. Epub 2021 Mar 23.

Laboratory of Transcription Dynamics, Research Center for Biological Visualization, Institute for Quantitative Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan; Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan. Electronic address:

Expression of the gap and pair-rule genes plays an essential role in body segmentation during Drosophila embryogenesis. However, it remains unclear how precise expression patterns of these key developmental genes arise from stochastic transcriptional activation at the single-cell level. Here, I employed genome-editing and live-imaging approaches to comprehensively visualize regulation of the gap and pair-rule genes at the endogenous loci. Read More

View Article and Full-Text PDF

sMicroRNA-28-5p acts as a metastasis suppressor in gastric cancer by targeting Nrf2.

Exp Cell Res 2021 May 15;402(2):112553. Epub 2021 Mar 15.

Clinic Research Institute of Zhanjiang, Central People's Hospital of Zhanjiang, Guangdong Medical University Zhanjiang Central Hospital, Zhanjiang, 524045, PR China. Electronic address:

The transcription factor nuclear factor (erythroid-2)-related factor 2 (Nrf2) can principally serve a mode of protection for both the normal cells and cancer cells from cellular stress, and elevates cancer cell survival. microRNA-28 (miR-28) has been involved in the regulation of Nrf2 expression in breast epithelial cells. However, no comprehensive analysis has been conducted regarding the function of miR-28-5p regulating Nrf2 in gastric cancer (GC). Read More

View Article and Full-Text PDF

Notch intracellular domain regulates glioblastoma proliferation through the Notch1 signaling pathway.

Oncol Lett 2021 Apr 19;21(4):303. Epub 2021 Feb 19.

Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

Notch intracellular domain (NICD), also known as the activated form of Notch1 is closely associated with cell differentiation and tumor invasion. However, the role of NICD in glioblastoma (GBM) proliferation and the underlying regulatory mechanism remains unclear. The present study aimed to investigate the expression of NICD and Notch1 downstream gene HES5 in human GBM and normal brain samples and to further detect the effect of NICD on human GBM cell proliferation. Read More

View Article and Full-Text PDF

Fasting-induced FOXO4 blunts human CD4 T helper cell responsiveness.

Nat Metab 2021 03 15;3(3):318-326. Epub 2021 Mar 15.

Laboratory of Mitochondrial Biology and Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.

Intermittent fasting blunts inflammation in asthma and rheumatoid arthritis, suggesting that fasting may be exploited as an immune-modulatory intervention. However, the mechanisms underpinning the anti-inflammatory effects of fasting are poorly characterized. Here, we show that fasting in humans is sufficient to blunt CD4 T helper cell responsiveness. Read More

View Article and Full-Text PDF

TGF-β-mediated Endothelial to Mesenchymal Transition (EndMT) and the Functional Assessment of EndMT Effectors using CRISPR/Cas9 Gene Editing.

J Vis Exp 2021 02 26(168). Epub 2021 Feb 26.

Dept. Cell Chemical Biology, Leiden University Medical Center; Oncode Institute, Leiden University Medical Center;

In response to specific external cues and the activation of certain transcription factors, endothelial cells can differentiate into a mesenchymal-like phenotype, a process that is termed endothelial to mesenchymal transition (EndMT). Emerging results have suggested that EndMT is causally linked to multiple human diseases, such as fibrosis and cancer. In addition, endothelial-derived mesenchymal cells may be applied in tissue regeneration procedures, as they can be further differentiated into various cell types (e. Read More

View Article and Full-Text PDF
February 2021

An innovative role for luteolin as a natural quorum sensing inhibitor in Pseudomonas aeruginosa.

Life Sci 2021 Jun 10;274:119325. Epub 2021 Mar 10.

Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China. Electronic address:

Aims: The emergence of antibiotic tolerance was a tricky problem in the treatment of chronic Pseudomonas aeruginosa-infected cystic fibrosis and burn victims. The quorum sensing (QS) inhibitor may serve as a new tactic for the bacterial resistance by inhibiting the biofilm formation and the production of virulence factors. This study explored the potential of luteolin as a QS inhibitor against P. Read More

View Article and Full-Text PDF

Exosomes from adipose-derived mesenchymal stem cells promote survival of fat grafts by regulating macrophage polarization via let-7c.

Acta Biochim Biophys Sin (Shanghai) 2021 Mar;53(4):501-510

Department of Plastic, Aesthetic and Maxillofacial Surgery, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.

The rate of fat graft survival is a critical aspect of successful surgery and has been a matter of concern for over 20 years. Owing to their anti-inflammatory effects and regenerative property, adipose-derived mesenchymal stem cells (AD-MSCs) have been adapted for clinical application in fat grafting, although the mechanism underlying their action remains unclear. Recently, exosomes derived from MSCs were suggested as a better alternative, and these exosomes have also been applied in diverse clinical therapies. Read More

View Article and Full-Text PDF

Oxidized low-density lipoprotein activates extracellular signal-regulated kinase signaling to downregulate sortilin expression in liver sinusoidal endothelial cells.

J Gastroenterol Hepatol 2021 Mar 10. Epub 2021 Mar 10.

Department of Endocrinology, Gansu Provincial Hospital, Lanzhou, China.

Background And Aim: Both type 2 diabetes mellitus and non-alcoholic fatty liver disease are closely associated with elevated levels of low-density lipoprotein cholesterol and its oxidized form (ox-LDL). This study aimed to investigate the regulation of sortilin in liver tissue and its potential implications for lipid metabolism.

Methods: Sixty male Wistar rats were randomly divided into four groups: control group (n = 15), ox-LDL group (n = 15), PD98059 group (n = 15), and ox-LDL + PD98059 group (n = 15). Read More

View Article and Full-Text PDF

Anti-androgenic effect of astaxanthin in LNCaP cells is mediated through the aryl hydrocarbon-androgen receptors cross talk.

J Food Biochem 2021 Apr 10;45(4):e13702. Epub 2021 Mar 10.

Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran.

The aim of this study was to investigate the anti-androgenic effects of astaxanthin (AST) on human prostatic cancer cell growth, and its impact on androgen receptor (AR) signaling using prostate cancer (PCa) cell line LNCaP. LNCaP cells were treated with AST alone and in combination with CH223191 and flutamide (Flu) in the presence and absence of testosterone. MTT assay, cellular prostate-specific antigen (PSA) and dihydrotestosterone (DHT) production, mRNA levels of CYP1A1, PSA, Kallikrein-Related Peptidase 2 (KLK2), Transmembrane Serine Protease 2 (TMPRSS2), and AR genes were measured as endpoints. Read More

View Article and Full-Text PDF

LncRNA, PLXDC2-OT promoted the osteogenesis potentials of MSCs by inhibiting the deacetylation function of RBM6/SIRT7 complex and OSX specific isoform.

Stem Cells 2021 Mar 8. Epub 2021 Mar 8.

Laboratory of Molecular Signaling and Stem Cells Therapy, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, People's Republic of China.

Bone regeneration and remodeling are complex physiological processes that are regulated by key transcription factors. Understanding the regulatory mechanism of key transcription factors on the osteogenic differentiation of mesenchymal stem cells (MSCs) is a key issue for successful bone regeneration and remodeling. In the present study, we investigated the regulatory mechanism of the histone deacetylase Sirtuin 7 (SIRT7) on the key transcription factor OSX and osteogenesis of MSCs. Read More

View Article and Full-Text PDF