28,498 results match your criteria down-regulated genes


Nutritional-status dependent effects of microplastics on activity and expression of alkaline phosphatase and alpha-amylase in Brachionus rotundiformis.

Sci Total Environ 2021 Sep 9;806(Pt 1):150213. Epub 2021 Sep 9.

College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, PR China.

Tissue-nonspecific alkaline phosphatase (ALPL) and alpha-amylase (AMY) are essential in the immune and digestive systems, respectively. Microplastics (MPs) pose a risk to zooplankton which may be in a state of feeding, starvation, or subsequent refeeding. However, molecular characterization of both enzymes and the regulated mechanisms affected by nutritional statuses and MPs remain unclear in zooplankton. Read More

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September 2021

Transcriptome analysis of host response to porcine epidemic diarrhea virus nsp15 in IPEC-J2 cells.

Microb Pathog 2021 Sep 24:105195. Epub 2021 Sep 24.

Institute of Pathogenic Microorganism and College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi, China. Electronic address:

Background: Porcine epidemic diarrhea virus (PEDV) is an enveloped positive-sense ssRNA virus which is highly lethal to piglets, causing enormous economic losses to swine industry worldwide. Nsp15 protein is an endoribonuclease of PEDV and plays an indispensable role in the viral proliferation. We reported the transcription files of nsp15 transfected IPEC-J2 cells for the first time to broaden our understanding of PEDV pathogenesis. Read More

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September 2021

Integrative small RNA and transcriptome analysis provides insight into key role of miR408 towards drought tolerance response in cowpea.

Plant Cell Rep 2021 Sep 27. Epub 2021 Sep 27.

Abiotic Stress and Agro-Biotechnology Lab, Institute of Life Sciences, Bhubaneswar, India.

Key Message: Drought stress response studies and overexpression of vun-miR408 proved it to be essential for abiotic stress tolerance in cowpea. Small RNA and transcriptome sequencing of an elite high-yielding drought-tolerant Indian cowpea cultivar, Pusa Komal revealed a differential expression of 198 highly conserved, 21 legume-specific, 14 less-conserved, and 10 novel drought-responsive microRNAs (miRNAs) along with 3391 (up-regulated) and 3799 (down-regulated) genes, respectively, in the leaf and root libraries. Among the differentially expressed miRNAs, vun-miR408-3p, showed an up-regulation of 3. Read More

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September 2021

Serum epithelial membrane protein 1 serves as a feasible biomarker in extrahepatic cholangiocarcinoma.

Int J Biol Markers 2021 Sep 27:17246008211035142. Epub 2021 Sep 27.

Department of Hepatobiliary Surgery, Chongqing University Three Gorges Hospital, China.

Background: Extrahepatic cholangiocarcinoma is a malignancy that originates from bile duct epithelium with an unfavorable prognosis. Epithelial membrane protein 1 was first discovered in 1995, functioning as an oncogene or anti-tumor gene in various cancers. However, the clinical role of epithelial membrane protein 1 extrahepatic cholangiocarcinoma remained unclear. Read More

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September 2021

The amino acid substitution affects cellular response to mistranslation.

G3 (Bethesda) 2021 Sep;11(10)

Department of Biochemistry, The University of Western Ontario, London, ON N6A 3K7, Canada.

Mistranslation, the misincorporation of an amino acid not specified by the "standard" genetic code, occurs in all organisms. tRNA variants that increase mistranslation arise spontaneously and engineered tRNAs can achieve mistranslation frequencies approaching 10% in yeast and bacteria. Interestingly, human genomes contain tRNA variants with the potential to mistranslate. Read More

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September 2021

Network Pharmacology and Experimental Validation Reveal the Effects of Chidamide Combined With Aspirin on Acute Myeloid Leukemia-Myelodysplastic Syndrome Cells Through PI3K/AKT Pathway.

Front Cell Dev Biol 2021 9;9:685954. Epub 2021 Sep 9.

Department of Hematology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Chidamide (CDM), a novel histone deacetylase inhibitor, is currently used for patients with peripheral T-cell lymphoma. Aspirin (ASA), an anti-inflammatory drug, has been shown to exert anticancer activity. Herein, we investigated the effect of CDM combined with ASA on myelodysplastic syndromes-derived acute myeloid leukemia (AML-MDS) cells and explored the underlying mechanism. Read More

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September 2021

Profiling of MicroRNAs in Midguts of Provides Novel Insights Into the Resistance.

Front Genet 2021 8;12:739849. Epub 2021 Sep 8.

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, China.

The diamondback moth (DBM), , one of the most destructive lepidopteran pests worldwide, has developed field resistance to (Bt) Cry toxins. Although miRNAs have been reported to be involved in insect resistance to multiple insecticides, our understanding of their roles in mediating Bt resistance is limited. In this study, we constructed small RNA libraries from midguts of the Cry1Ac-resistant (Cry1S1000) strain and the Cry1Ac-susceptible strain (G88) using a high-throughput sequencing analysis. Read More

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September 2021

GH Overexpression Alters Spermatic Cells MicroRNAome Profile in Transgenic Zebrafish.

Front Genet 2021 8;12:704778. Epub 2021 Sep 8.

Laboratório de Genômica Estrutural, Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Brazil.

Overexpression of growth hormone (GH) in -transgenic zebrafish of a highly studied lineage F0104 has earlier been reported to cause increased muscle growth. In addition to this, GH affects a broad range of cellular processes in transgenic fish, such as morphology, physiology, and behavior. Reports show changes such as decreased sperm quality and reduced reproductive performance in transgenic males. Read More

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September 2021

Gibberellin Induced Transcriptome Profiles Reveal Gene Regulation of Loquat Flowering.

Front Genet 2021 10;12:703688. Epub 2021 Sep 10.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Horticulture, South China Agricultural University, Guangzhou, China.

Flowering is an integral part of the life cycle of flowering plants, which is essential for plant survival and crop production. Most woody fruit trees such as apples and pears bloom in spring, but loquat blooms in autumn and winter. Gibberellin (GA) plays a key role in the regulation of plant flower formation. Read More

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September 2021

ONAC066, A Stress-Responsive NAC Transcription Activator, Positively Contributes to Rice Immunity Against Through Modulating Expression of and Three Cytochrome P450 Genes.

Front Plant Sci 2021 9;12:749186. Epub 2021 Sep 9.

National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, China.

NAC transcriptional factors constitute a large family in rice and some of them have been demonstrated to play crucial roles in rice immunity. The present study investigated the function and mechanism of in rice immunity. ONAC066 shows transcription activator activity that depends on its C-terminal region in rice cells. Read More

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September 2021

Transcriptome-based analysis of blood samples reveals elevation of DNA damage response, neutrophil degranulation, cancer and neurodegenerative pathways in Plasmodium falciparum patients.

Malar J 2021 Sep 26;20(1):383. Epub 2021 Sep 26.

Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich-Heine University, 40225, Düsseldorf, Germany.

Background: Malaria caused by Plasmodium falciparum results in severe complications including cerebral malaria (CM) especially in children. While the majority of falciparum malaria survivors make a full recovery, there are reports of some patients ending up with neurological sequelae or cognitive deficit.

Methods: An analysis of pooled transcriptome data of whole blood samples derived from two studies involving various P. Read More

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September 2021

Identification of Important Genes Related to Ferroptosis and Hypoxia in Acute Myocardial Infarction Based on WGCNA.

Bioengineered 2021 Sep 26. Epub 2021 Sep 26.

Department of cardio-thoracis surgery, Zhejiang Hospital, Hangzhou, Zhejiang, China.

Acute myocardial infarction (AMI) tends to cause severe heart failure and the population suffering from AMI gradually become younger. This study aims to determine the key genes associated with AMI, ferroptosis and hypoxia that could serve as novel biomarkers or potential therapeutic targets for AMI. There were 522 up-regulated genes and 119 down-regulated genes in GSE4648. Read More

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September 2021

Depletion of β3-adrenergic receptor relieves pressure overload-induced cardiac hypertrophy and heart failure via enhancing innate immune response.

Biomed Pharmacother 2021 Sep 23;143:112194. Epub 2021 Sep 23.

Department of Cardiovascular medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address:

Cardiac pressure overload is a crucial risk factor for cardiac hypertrophy and heart failure. Our previous study showed that depletion of the β3-adrenergic receptor (ADRB3) induced left ventricular diastolic dysfunction via potential regulation of energy metabolism and cardiac contraction. However, the effects of ADRB3 on pressure overload-induced heart failure remain unclear. Read More

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September 2021

Baicalin inhibits biofilm formation by influencing primary adhesion and aggregation phases in Staphylococcus saprophyticus.

Vet Microbiol 2021 Sep 23;262:109242. Epub 2021 Sep 23.

MOE Joint International Research Laboratory of Animal Health and Food Safety and Traditional Chinese Veterinary Medicine Research Center, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address:

The ability to form biofilms on surfaces makes Staphylococcus saprophyticus (S. saprophyticus) becomes the main pathogenic factor in nosocomial infections. Previously, we demonstrated that baicalin (Bac) inhibited azithromycin-resistant S. Read More

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September 2021

Polysaccharides from Volvariella volvacea inhibit fat accumulation in C. elegans dependent on the aak-2/nhr-49-mediated pathway.

J Food Biochem 2021 Sep 25:e13912. Epub 2021 Sep 25.

Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun, China.

Volvariella volvacea has bioactivities in improving immunity, anti-oxidation, and alleviating obesity, which is an excellent functional food. Polysaccharide from Volvariella volvacea (VPS), one of the main bioactive components, exerts a potential fat-lowering effect, but its exact mechanism remains unclear. In this study, the effects and molecular pathways of VPS regulate the fat deposition of Caenorhabditis elegans. Read More

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September 2021

LINC00662 facilitates osteosarcoma progression via sponging miR-103a-3p and regulating SIK2 expression.

J Tissue Eng Regen Med 2021 Sep 24. Epub 2021 Sep 24.

Shengli Clinical Medical College of Fujian Medical University, Department of Orthopedics, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, 350001, Fujian, China, Fuzhou, 350001, Fujian, China.

Aims: Long non-coding RNA (lncRNA) involvement in regulating assorted cancers has been determined. Long intergenic non-protein coding RNA 662 (LINC00662) has been studied in gastric cancer. However, its function was not elucidated in osteosarcoma (OS). Read More

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September 2021

ZmCTLP1 is required for the maintenance of lipid homeostasis and the basal endosperm transfer layer in maize kernels.

New Phytol 2021 Sep 24. Epub 2021 Sep 24.

State Key Laboratory of Plant Physiology and Biochemistry and National Maize Improvement Center, Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, P. R. China.

Maize kernel weight is influenced by the unloading of nutrients from the maternal placenta and their passage through the transfer tissue of the basal endosperm transfer layer (BETL) and the basal intermediate zone (BIZ) to the upper part of the endosperm. Here, we show that Small kernel 10 (Smk10) encodes a choline transporter-like protein 1 (ZmCTLP1) that facilitates choline uptake and is located in the trans-Golgi network (TGN). Its loss-of-function results in reduced choline content, leading to smaller kernels with a lower starch content. Read More

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September 2021

Can only one physiological trait determinate the adverse effect of green fluorescent protein (GFP) incorporation on Vibrio virulence?

Appl Microbiol Biotechnol 2021 Sep 24. Epub 2021 Sep 24.

Laboratory of Aquaculture & Artemia Reference Center, Department of Animal Sciences and Aquatic Ecology, Faculty of Bioscience Engineering, Ghent University, 9000, Ghent, Belgium.

Green fluorescent protein (GFP) has been used extensively for in situ animal studies that follow up bacterial infection under epifluorescence microscopy. It is assumed that GFP is acting as a "neutral" protein with no influence on the bacterial physiology. To verify this hypothesis, the virulence of Vibrio splendidus ME9, Vibrio anguillarum NB10, and their respective GFP-tagged strains ME9-GFP and NB10-GFP (transconjugants) was compared in vitro and tested in vivo towards blue mussel (Mytilus edulis) larvae. Read More

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September 2021

RORα Enhances Lysosomal Acidification and Autophagic Flux in the Hepatocytes.

Hepatol Commun 2021 Aug 24. Epub 2021 Aug 24.

College of Pharmacy, Seoul National University, Seoul, Korea.

Lysosomes are intracellular acidic organelles with catabolic functions that contribute to the activation of autophagy. Although autophagy abnormality is associated with defects in lysosomal acidification during the progression of nonalcoholic fatty liver disease (NAFLD), the mechanisms of control of lysosomal acidification are not well understood at the molecular level. Thus, we aimed to elucidate the role of the orphan nuclear receptor retinoic acid-related orphan receptor α (RORα) in lysosomal acidification and autophagic flux, particularly in nutrition-enriched hepatocytes. Read More

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Transcriptomic Cross-Species Analysis of Chronic Liver Disease Reveals Consistent Regulation Between Humans and Mice.

Hepatol Commun 2021 Aug 28. Epub 2021 Aug 28.

Systems Toxicology, Leibniz Research Centre for Working Environment and Human Factors at the Technical University Dortmund, Dortmund, Germany.

Mouse models are frequently used to study chronic liver diseases (CLDs). To assess their translational relevance, we quantified the similarity of commonly used mouse models to human CLDs based on transcriptome data. Gene-expression data from 372 patients were compared with data from acute and chronic mouse models consisting of 227 mice, and additionally to nine published gene sets of chronic mouse models. Read More

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Expression profiles of tRNA-derived small RNA and their potential roles in oral submucous fibrosis.

J Oral Pathol Med 2021 Sep 24. Epub 2021 Sep 24.

Centre of Stomatology, Xiangya Hospital, Central South University, Changsha, Hunan, China.

Background: Although transfer RNA (tRNA) has been found to be the main source of a rich class of non-coding RNA. Moreover, the tRNA-derived small RNA (tsRNA) has been proved to play an irreplaceable role in the human body, and its dynamic imbalance could affect the progress of the disease. However, the research on tsRNA in oral submucous fibrosis (OSF) is still scarce. Read More

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September 2021

Transcriptome and Oxylipin Profiling Joint Analysis Reveals Opposite Roles of 9-Oxylipins and Jasmonic Acid in Maize Resistance to Gibberella Stalk Rot.

Front Plant Sci 2021 7;12:699146. Epub 2021 Sep 7.

State Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.

Gibberella stalk rot caused by is one of the devastating diseases of maize that causes significant yield losses worldwide. The molecular mechanisms regulating defense against this pathogen remain poorly understood. According to recent studies, a major oxylipin hormone produced by 13-lipoxygenases (LOX) namely jasmonic acid (JA) has been associated with maize susceptibility to GSR. Read More

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September 2021

Identification and Characterization of a Germline Mutation in CARD11 From a Chinese Case of B Cell Expansion With NF-κB and T Cell Anergy.

Front Immunol 2021 7;12:676386. Epub 2021 Sep 7.

Precision Medical Center, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology, Wuhan, China.

B cell expansion with NF-κB and T cell anergy (BENTA) is a rare primary immunodeficiency disorder caused by gain-of-function (GOF) mutations in the gene. Affected patients present with persistent B cell lymphocytosis in early childhood paired with lymphadenopathy and splenomegaly. Until now only six activating mutations from 14 patients have been reported in . Read More

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September 2021

Identification of Prognosis-Associated Biomarkers in Thyroid Carcinoma by a Bioinformatics Analysis.

Authors:
Yong Qin

Int J Gen Med 2021 16;14:5737-5747. Epub 2021 Sep 16.

Department of Thyroid Surgery, Bishan Hospital of Chongqing, Chongqing, 402760, People's Republic of China.

Background: This study aimed to identify the key genes associated with prognosis in thyroid cancer (TC), and to explore potential pathways.

Methods: GSE66783, GSE58545, and GSE129562 datasets were used to identify differentially expressed genes (DEGs) between tumor and normal tissues, followed by KEGG analyses on DEGs. The protein-protein interaction (PPI) network of DEGs was subsequently constructed to find the top 10 hub genes and seed genes in the whole network. Read More

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September 2021

Dietary chromium modulates glucose homeostasis and induces oxidative stress in Pacific white shrimp (Litopenaeus vannamei).

Aquat Toxicol 2021 Sep 14;240:105967. Epub 2021 Sep 14.

Laboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo, 315211, China. Electronic address:

While chromium (Cr) has been recognized as an essential nutrient for all animals, and dietary supplementation can be beneficial, it can also be toxic. The present study aimed to investigate the contrasting effects of dietary chromium in Pacific white shrimp Litopenaeus vannamei. Five experimental diets were formulated to contain Cr at levels of 0. Read More

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September 2021

Bioinformatics analyses of gene expression profile identify key genes and functional pathways involved in cutaneous lupus erythematosus.

Clin Rheumatol 2021 Sep 23. Epub 2021 Sep 23.

Department of Rheumatology and Immunology, The Affiliated Hospital of Hubei Minzu University, Enshi, Hubei Province, China.

Background: Lupus erythematosus is an autoimmune disease that causes damage to multiple organs ranging from skin lesions to systemic manifestations. Cutaneous lupus erythematosus (CLE) is a common type of lupus erythematosus (LE), but its molecular mechanisms are currently unknown. The study aimed to explore changes in the gene expression profiles and identify key genes involved in CLE, hoping to uncover its molecular mechanism and identify new targets for CLE. Read More

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September 2021

Novel Insight Into Glycosaminoglycan Biosynthesis Based on Gene Expression Profiles.

Front Cell Dev Biol 2021 6;9:709018. Epub 2021 Sep 6.

Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.

Glycosaminoglycans (GAGs) including chondroitin sulfate, dermatan sulfate, heparan sulfate, and keratan sulfate, except for hyaluronan that is a free polysaccharide, are covalently attached to core proteins to form proteoglycans. More than 50 gene products are involved in the biosynthesis of GAGs. We recently developed a comprehensive glycosylation mapping tool, GlycoMaple, for visualization and estimation of glycan structures based on gene expression profiles. Read More

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September 2021

A small molecule II-6s inhibits biofilms.

J Oral Microbiol 2021 14;13(1):1978756. Epub 2021 Sep 14.

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

Background: Limitations of current intracanal irrigants such as sodium hypochlorite (NaOCl) and chlorhexidine (CHX) necessitate the development of novel antimicrobial agents to control endodontic infection.

Aim: This study investigated the antimicrobial activities of a small molecule II-6s against associated with endodontic diseases.

Methods: The susceptibility of to II-6s was evaluated by the microdilution method and time-kill assay. Read More

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September 2021

Transcriptome Analysis of Immune Receptor Activation and Energy Metabolism Reduction as the Underlying Mechanisms in Interleukin-6-Induced Skeletal Muscle Atrophy.

Front Immunol 2021 6;12:730070. Epub 2021 Sep 6.

Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-Innovation Center of Neuroregeneration, Nantong University, National Medical Products Administration Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair, Nantong, China.

Background: Inflammation may trigger skeletal muscle atrophy induced by cancer cachexia. As a pro-inflammatory factor, interleukin-6 may cause skeletal muscle atrophy, but the underlying molecular mechanisms have not been explored.

Methods: In this experimental study, we used adult male ICR mice, weighing 25 ± 2 g, and the continuous infusion of interleukin-6 into the tibialis anterior muscle to construct a skeletal muscle atrophy model (experimental group). Read More

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September 2021

Association of Pericardiac Adipose Tissue With Coronary Artery Disease.

Front Endocrinol (Lausanne) 2021 6;12:724859. Epub 2021 Sep 6.

Department of Cardiology, Huadong Hospital Affiliated to Fudan University, Shanghai, China.

Background And Aim: Coronary artery disease (CAD) poses a worldwide health threat. Compelling evidence shows that pericardial adipose tissue (PAT), a brown-like adipose adjacent to the external surface of the pericardium, is associated with CAD. However, the specific molecular mechanisms of PAT in CAD are elusive. Read More

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September 2021