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Short-term running exercise alters DNA methylation patterns in neuronal nitric oxide synthase and brain-derived neurotrophic factor genes in the mouse hippocampus and reduces anxiety-like behaviors.

FASEB J 2021 Aug;35(8):e21767

The Fukuoka University Institute for Physical Activity, Fukuoka University, Fukuoka, Japan.

Running exercise has beneficial effects on brain health. However, the effects of relatively short-term running exercise (STEx) on behavior, and its underlying signaling pathways, are poorly understood. In this study, we evaluated the possibility that the regulation by STEx of brain-derived neurotrophic factor (BDNF) and neuronal nitric oxide synthase (nNOS, encoded by NOS1), which are important molecules for anxiety regulation, might involve mechanisms of epigenetic modification, such as DNA methylation. Read More

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Parent-Child Agreement on Quality of Life in Children With Hearing Loss Using the HEAR-QL.

Otol Neurotol 2021 Jul 28. Epub 2021 Jul 28.

Washington University School of Medicine, Missouri Department of Otolaryngology - Head and Neck Surgery, Washington University School of Medicine, St. Louis, MO.

Objective: We explored whether children with hearing loss (HL) and their parents perceive the child's quality of life (QOL) similarly using the Hearing Environment And Reflections on Quality of Life (HEAR-QL) survey.

Study Design: Cross-sectional study.

Setting: Pediatric otolaryngology clinic. Read More

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NUDCD1 knockdown inhibits the proliferation, migration, and invasion of pancreatic cancer via the EMT process.

Aging (Albany NY) 2021 Jul 29;13(undefined). Epub 2021 Jul 29.

School of Stomatology, Wenzhou Medical University, Wenzhou 110013, China.

NudC domain containing 1 (NUDCD1) is an oncoprotein frequently activated or upregulated in various human cancers, but its role in pancreatic cancer (PC) remains unknown. Thus, we aimed to determine the function and mechanism of NUDCD1 in PC. We employed Western blot and quantitative real-time polymerase chain reaction to assess NUDCD1 expression in cells and PC tissues. Read More

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Simple monitoring of endocrine-disrupting chemicals using transgenic Arabidopsis plants expressing medaka estrogen receptor.

Chemosphere 2021 Jul 22;286(Pt 1):131633. Epub 2021 Jul 22.

Biosignal Research Center, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo, 657-8501, Japan; Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo, 657-8501, Japan. Electronic address:

Endocrine-disrupting chemicals (EDCs) are widespread contaminants that severely affect the endocrine systems of living organisms. In addition to the conventional instrument-based approaches for quantifying organic pollutants, a monitoring method using transgenic plants has also been proposed. Plants carrying a recombinant receptor gene combined with a reporter gene represent a system for the easy detection of ligands that specifically bind to the receptor molecule. Read More

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The impact of tyrosine kinase inhibitors on allogeneic hematopoietic stem cell transplantation for adult patients with Philadelphia chromosome-positive acute lymphoblastic leukemia.

Leuk Res 2021 Jun 18;109:106647. Epub 2021 Jun 18.

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 288 Nanjing Road, Heping District, Tianjin, 300020, China. Electronic address:

In the tyrosine kinase inhibitor (TKI) era, allogeneic hematopoietic stem cell transplantation (allo-HSCT) is still the most potential approach for cure of adult patients with Philadelphia chromosome-positive acute lymphocytic leukemia (Ph ALL). TKI plus chemotherapy has strikingly increased response rates and depth of response, and facilitated allo-HSCT, which decreases relapse and improves survival eventually. Meanwhile, for those with older age or comorbidities at diagnosis, TKI in combination with reduced-intensity chemotherapy or chemotherapy-free strategy reduces treatment-related mortality, deferred intensive chemotherapy increases molecular responses and reduced-intensity conditioning (RIC) allo-HSCT improves survival finally. Read More

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Evaluation of Psychometric Properties of the Persian Version of Brief Male Sexual Function Inventory: A Cross-Sectional Study.

Sex Med 2021 Jul 26;9(5):100409. Epub 2021 Jul 26.

Non-Communicable Diseases Research Center, Ilam University of Medical Sciences, Ilam, Iran.

Introduction: Male sexual dysfunction is a common problem, and there are many self-report questionnaires for measuring sexual function among men; however, the Brief Male Sexual Function Inventory (BSFI) is a tool that has 5 subscales, which is more complete than others. a validated self-report questionnaire, in the local language with modest expressions is required for men.

Aim: To determine the validity and reliability of the Persian version of the BSFI among men. Read More

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Application of terahertz spectroscopy combined with density functional theory to analysis of intermolecular weak interactions for coumarin and 6-methylcoumarin.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Jul 21;263:120159. Epub 2021 Jul 21.

Guangxi Key Laboratory of Automatic Detecting Technology and Instruments, School of Electronic Engineering and Automation, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China. Electronic address:

The terahertz (THz) absorption spectra of coumarin and 6-methylcoumarin have been investigated by terahertz time-domain spectroscopy (THz-TDS) system in the frequency range from 0.4 to 2.8 THz. Read More

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Semi-supervised learning with progressive unlabeled data excavation for label-efficient surgical workflow recognition.

Med Image Anal 2021 Jul 8;73:102158. Epub 2021 Jul 8.

Department of Computer Science and Engineering, The Chinese University of Hong Kong, Hong Kong; T Stone Robotics Institute, The Chinese University of Hong Kong, Hong Kong.

Surgical workflow recognition is a fundamental task in computer-assisted surgery and a key component of various applications in operating rooms. Existing deep learning models have achieved promising results for surgical workflow recognition, heavily relying on a large amount of annotated videos. However, obtaining annotation is time-consuming and requires the domain knowledge of surgeons. Read More

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A non-RBM targeted RBD specific antibody neutralizes SARS-CoV-2 inducing S1 shedding.

Biochem Biophys Res Commun 2021 Jul 20;571:152-158. Epub 2021 Jul 20.

Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing, 400010, China; Chongqing Key Laboratory of Basic and Translational Research of Tumor Immunology, Chongqing Medical University, Chongqing, 400010, China. Electronic address:

Potent neutralizing antibodies (Abs) have been proven with therapeutic efficacy for the intervention against SARS-CoV-2. Majority of these Abs function by directly interfering with the virus entry to host cells. Here, we identified a receptor binding domain (RBD) specific monoclonal Ab (mAb) 82A6 with efficient neutralizing potency against authentic SARS-CoV-2 virus. Read More

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Dynamin 2 and BAR domain protein pacsin 2 cooperatively regulate formation and maturation of podosomes.

Biochem Biophys Res Commun 2021 Jul 26;571:145-151. Epub 2021 Jul 26.

Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8558, Japan. Electronic address:

Podosomes are actin-rich adhesion structures formed in a variety of cell types, such as monocytic cells or cancer cells, to facilitate attachment to and degradation of the extracellular matrix (ECM). Previous studies showed that dynamin 2, a large GTPase involved in membrane remodeling and actin organization, is required for podosome function. However, precise roles of dynamin 2 at the podosomes remain to be elucidated. Read More

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Variable length particle swarm optimization and multi-feature deep fusion for motor imagery EEG classification.

Biochem Biophys Res Commun 2021 Jul 26;571:131-136. Epub 2021 Jul 26.

School of Control Science and Engineering, Tiangong University, Tianjin, 300387, China. Electronic address:

Brain-computer interfaces are a new pathway for communication between human body and the external environment. High classification accuracy for motor imagery electroencephalogram (EEG) signals is desirable by improving the algorithm of feature extraction and classification. A novel algorithm (VLPSO-MFDF) based on the variable length particle swarm optimization (VLPSO) and multi-feature deep fusion (MFDF) is proposed. Read More

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Chitosan derivatives: A suggestive evaluation for novel inhibitor discovery against wild type and variants of SARS-CoV-2 virus.

Int J Biol Macromol 2021 Jul 26. Epub 2021 Jul 26.

Department of Biotechnology, National Institute of Technology (NIT), Raipur, Chhattisgarh, India. Electronic address:

With increasing global cases and mortality rates due to COVID-19 infection, finding effective therapeutic interventions has become a top priority. Marine resources are not explored much and to be taken into consideration for exploring antiviral potential. Chitosan (carbohydrate polymer) is one such bioactive glycan found ubiquitously in marine organisms. Read More

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Identification and function of an Arasin-like peptide from Litopenaeus vannamei.

Dev Comp Immunol 2021 Jul 26:104174. Epub 2021 Jul 26.

College of Fisheries, Guangdong Ocean University, Zhanjiang, PR China. Electronic address:

Antimicrobial peptides (AMPs) play an important role in the host defense system of shrimps. In this study, an Arasin-like peptide, named as LvArasin-like, was identified from the hemocytes of the pacific white shrimp, Litopenaeus vannamei. The complete open reading frame (ORF) of LvArasin-like was 213 bp, encoding 70 amino acid residues with a predicted molecular mass of 5. Read More

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Computational and experimental characterization of isomers of escin -induced renal cytotoxicity by inhibiting heat shock proteins.

Eur J Pharmacol 2021 Jul 26:174372. Epub 2021 Jul 26.

State Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China. Electronic address:

Escin is a natural mixture of triterpene saponins, exhibits anti-oedematous properties and promotes venous drainage by oral administration or injection. Upon clinical application of escin, adverse kidney reactions have been reported and the nephrotoxic mechanism responsible for this reaction remains elusive. In the present study, four isomeric escins (β-form: escin Ia and escin Ib; α-form: isoescin Ia and isoescin Ib) were found severely decreasing the cell viability of human kidney (HK-2) cells. Read More

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Two novel Mutations of the LPL Gene in two Chinese family cases with Familial Chylomicronemia Syndrome.

Clin Chim Acta 2021 Jul 26. Epub 2021 Jul 26.

Kunming Key Laboratory of Children Infection and Immunity, Yunnan Key Laboratory of Children's Major Disease Research, Yunnan Medical Center for Pediatric Diseases, Yunnan Institute of Pediatrics, Kunming Children's Hospital, Kunming 650228, Yunnan, China. Electronic address:

The aim of this study was to investigate the clinical features and genetic causes of two family cases with familial chylomicronemia syndrome (FCS). Clinical manifestations of proband 1 and her families, and also proband 2 showed severe hypertriglyceridemia, especially the triglycerides levels of two probands were extremely high. Gene sequencing results showed that the LPL genes in each of the two probands had a new mutation site. Read More

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Safety, tolerability, and immunogenicity of an aerosolised adenovirus type-5 vector-based COVID-19 vaccine (Ad5-nCoV) in adults: preliminary report of an open-label and randomised phase 1 clinical trial.

Lancet Infect Dis 2021 Jul 26. Epub 2021 Jul 26.

Beijing Institute of Biotechnology, Academy of Military Medical Sciences, Beijing, China.

Background: SARS-CoV-2 has caused millions of deaths, and, since Aug 11, 2020, 20 intramuscular COVID-19 vaccines have been approved for use. We aimed to evaluate the safety and immunogenicity of an aerosolised adenovirus type-5 vector-based COVID-19 vaccine (Ad5-nCoV) in adults without COVID-19 from China.

Method: This was a randomised, single-centre, open-label, phase 1 trial done in Zhongnan Hospital (Wuhan, China), to evaluate the safety and immunogenicity of the Ad5-nCoV vaccine by aerosol inhalation in adults (≥18 years) seronegative for SARS-CoV-2. Read More

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Phospholipid exchange shows insulin receptor activity is supported by both the propensity to form wide bilayers and ordered raft domains.

J Biol Chem 2021 Jul 26:101010. Epub 2021 Jul 26.

Dept. of Biochemistry and Cell Biology Stony Brook University, Stony Brook, NY, 11794, USA. Electronic address:

Insulin receptor (IR) is a membrane tyrosine kinase that mediates the response of cells to insulin. IR activity has been shown to be modulated by changes in plasma membrane lipid composition, but the properties and structural determinants of lipids mediating IR activity are poorly understood. Here, using efficient methyl-alpha-cyclodextrin mediated lipid exchange, we studied the effect of altering plasma membrane outer leaflet phospholipid composition upon the activity of IR in mammalian cells. Read More

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Preparation of the ubiquitination-triggered active form of SETDB1 in E. coli for biochemical and structural analyses.

J Biochem 2021 Jul 29. Epub 2021 Jul 29.

Structural Biology Laboratory, Graduate School of Medical Life Science, Yokohama City University, 1-7-29, Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.

Trimethylation of histone H3 at K9 by the lysine methyltransferase, SET domain bifurcated histone lysine methyltransferase 1 (SETDB1) plays a pivotal role in silencing tissue-specific genes and retrotransposable elements. In mammalian cells, SETDB1 undergoes monoubiquitination in the insertion region of the SET domain in an E3 ubiquitin ligase-independent manner. This ubiquitination has been shown to enhance the histone H3-K9 methyltransferase activity of SETDB1; however, the molecular mechanism underlying SETDB1 activation by ubiquitination is unknown. Read More

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Analog hardware trojan design and detection in OFDM based wireless cryptographic ICs.

PLoS One 2021 29;16(7):e0254903. Epub 2021 Jul 29.

Department of Information and Communication Technology (ICT), Institute of Information and Communication Technology (IICT), Bangladesh University of Engineering and Technology (BUET), Dhaka, Bangladesh.

Due to Hardware Trojan (HT), trustworthiness of Integrated Circuit (IC) supply chain is a burning issue in Semiconductor Industry nowadays. Over the last decade, extensive research has been carried on HT detection methods for digital circuits. However, the HT issue remains largely unexplored in the domain of Analog Mixed Signal (AMS)/ RF circuit where it is now an appealing target for the attackers. Read More

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RNA polymerases in strict endosymbiont bacteria with extreme genome reduction show distinct erosions that might result in limited and differential promoter recognition.

PLoS One 2021 29;16(7):e0239350. Epub 2021 Jul 29.

Biological Engineering Laboratory, Genetic Engineering Department, Center for Research and Advanced Studies of the National Polytechnic Institute, Irapuato Gto, México.

Strict endosymbiont bacteria present high degree genome reduction, retain smaller proteins, and in some instances, lack complete functional domains compared to free-living counterparts. Until now, the mechanisms underlying these genetic reductions are not well understood. In this study, the conservation of RNA polymerases, the essential machinery for gene expression, is analyzed in endosymbiont bacteria with extreme genome reductions. Read More

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MeCP2 is a microsatellite binding protein that protects CA repeats from nucleosome invasion.

Science 2021 Jun;372(6549)

Institute of Genetics and of Molecular and Cellular Biology (IGBMC), 67400 Illkirch, France.

The Rett syndrome protein MeCP2 was described as a methyl-CpG-binding protein, but its exact function remains unknown. Here we show that mouse MeCP2 is a microsatellite binding protein that specifically recognizes hydroxymethylated CA repeats. Depletion of MeCP2 alters chromatin organization of CA repeats and lamina-associated domains and results in nucleosome accumulation on CA repeats and genome-wide transcriptional dysregulation. Read More

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Brain tumor classification using fine-tuned GoogLeNet features and machine learning algorithms: IoMT enabled CAD system.

IEEE J Biomed Health Inform 2021 Jul 29;PP. Epub 2021 Jul 29.

In the healthcare research community, Internet of Medical Things (IoMT) is transforming the healthcare system into the world of the future internet. In IoMT enabled Computer aided diagnosis (CAD) system, the Health-related information is stored via the internet, and supportive data is provided to the patients. The development of various smart devices is interconnected via the internet, which helps the patient to communicate with a medical expert using IoMT based remote healthcare system for various life threatening diseases, e. Read More

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Urinary Bladder Volume Monitoring Using Magnetic Induction Tomography: A Rotational Simulation Model for Anatomical Slices within the Pelvic Region.

IEEE Trans Biomed Eng 2021 Jul 29;PP. Epub 2021 Jul 29.

Urinary bladder volume monitoring can benefit from contactless measurements, as alternative to the traditional medical methods of transurethral catheterization or ultrasound examination. The emerging modality of Magnetic Induction Tomography (MIT) offers the possibility for estimation of the intravesical volume in the physiological and pathological states using conductivity map reconstructions of the tissues present in the pelvic region. Within MIT, eddy currents originating from the conductive urine can produce their own magnetic field in response to an external magnetic source that is susceptible of being detected outside the body by means of a static ring of sensing coils. Read More

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Comparison of immunogenicity and reactogenicity of inactivated SARS-CoV-2 vaccine (CoronaVac) in previously SARS-CoV-2 infected and uninfected health care workers.

Hum Vaccin Immunother 2021 Jul 29:1-5. Epub 2021 Jul 29.

Memorial Ataşehir Hospital, Clinic of Pediatrics, İstanbul, Turkey.

The effects of inactivated SARS-CoV-2 vaccine (CoronaVac) on previously naturally infected individuals are unknown. This study compared immunogenicity and reactogenicity of CoronaVac in once naturally infected health-care workers (HCWs) and uninfected HCWs. All HCWs were immunized with two doses of CoronaVac (600 U/0. Read More

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Serial administration of rhBMP-2 and alendronate enhances the differentiation of osteoblasts.

Int J Rheum Dis 2021 Jul 29. Epub 2021 Jul 29.

Division of Rheumatology, Department of Internal Medicine, Keimyung University School of Medicine, Daegu, Korea.

Aim: The incorporation of growth factors is an effective strategy to accelerate bone induction. Bone morphogenetic protein-2 (BMP-2) promotes osteoblast differentiation and induces bone formation. Alendronate (ALN) is an osteoclast deactivation drug. Read More

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Protein oligomer modeling guided by predicted inter-chain contacts in CASP14.

Proteins 2021 Jul 29. Epub 2021 Jul 29.

Department of Biochemistry, University of Washington, Seattle, WA, USA.

For CASP14, we developed deep learning-based methods for predicting homo-oligomeric and hetero-oligomeric contacts and used them for oligomer modeling. To build structure models, we developed an oligomer structure generation method that utilizes predicted inter-chain contacts to guide iterative restrained minimization from random backbone structures. We supplemented this gradient-based fold-and-dock method with template-based and ab initio docking approaches using deep learning-based subunit predictions on 29 assembly targets. Read More

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BUD23-TRMT112 interacts with the L protein of Borna disease virus and mediates the chromosomal tethering of viral ribonucleoproteins.

Microbiol Immunol 2021 Jul 29. Epub 2021 Jul 29.

Laboratory of RNA Viruses, Institute for Frontier Life and Medical Sciences (inFRONT), Kyoto University, Kyoto.

Persistent intranuclear infection is an uncommon infection strategy among RNA viruses. However, Borna disease virus 1 (BoDV-1), a nonsegmented, negative-strand RNA virus, maintains viral infection in the cell nucleus by forming structured aggregates of viral ribonucleoproteins (vRNPs), and by tethering these vRNPs onto the host chromosomes. To better understand the nuclear infection strategy of BoDV-1, we determined the host protein interactors of the BoDV-1 large (L) protein. Read More

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SARS-CoV-2 pseudovirus infectivity and expression of viral entry-related factors ACE2, TMPRSS2, Kim-1 and NRP-1 in human cells from respiratory, urinary, digestive, reproductive and immune systems.

J Med Virol 2021 Jul 29. Epub 2021 Jul 29.

Cancer Virology Program, UPMC Hillman Cancer Center and Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

Infection by SARS-CoV-2 causes a wide spectrum of syndromes involving multiple organ systems, and is primarily mediated by viral spike (S) glycoprotein through the receptor binding domain (RBD) and numerous cellular proteins including ACE2, TMPRSS2, Kim-1 and NRP-1. In this study, we examined the entry tropism of SARS-CoV-2 and SARS-CoV using S protein-based pseudoviruses to infect 22 cell lines and 3 types of primary cells isolated from respiratory, urinary, digestive, reproductive and immune systems. At least 1 cell line or type of primary cells from each organ system was infected by both pseudoviruses. Read More

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A Novel Therapeutic Peptide Blocks SARS-CoV-2 Spike Protein Binding with Host Cell ACE2 Receptor.

Drugs R D 2021 Jul 29. Epub 2021 Jul 29.

Department of Biosciences and Biomedical Engineering (BSBE), Indian Institute of Technology Indore (IITI), Simrol, Indore, 453552, India.

Background And Objective: Coronavirus disease 2019 is a novel disease caused by the severe acute respiratory syndrome coronavirus (SARS-CoV)-2 virus. It was first detected in December 2019 and has since been declared a pandemic causing millions of deaths worldwide. Therefore, there is an urgent need to develop effective therapeutics against coronavirus disease 2019. Read More

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Agreement Between Spectral-Domain and Swept-Source Optical Coherence Tomography Retinal Thickness Measurements in Macular and Retinal Disease.

Ophthalmol Ther 2021 Jul 29. Epub 2021 Jul 29.

NIHR Biomedical Research Centre at Moorfields Eye Hospital NHS Foundation Trust, London, Institute of Ophthalmology, University College London, 162 City Road, London, EC1V 2PD, UK.

Introduction: To assess inter-device agreement in optical coherence tomography-derived retinal thickness measurements in patients with known macular conditions between spectral-domain and swept-source optical coherence tomography (OCT).

Methods: Two hundred seventy-two subjects were included in the study. They consisted of 91 male (33. Read More

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